A solid phase method detector and detection method

Abstract

The present invention relates to the field of biological experiments, and in particular to a solid phase method detector and a detection method, comprising a housing, a sample loading mechanism and a sample loading platform are arranged in the housing, and the sample loading mechanism is arranged above the sample loading platform, and a card compartment for providing and recovering a reagent card is arranged on one side of the housing; and the card compartment is connected to the housing; the sample loading mechanism is used to transfer the reagent card from the card compartment to the sample loading platform or transfer the reagent card from the sample loading platform to the card compartment, and complete the liquid transfer of the sample to the dry chemical reagent card; the sample loading platform is used to place test tubes and reagent cards. The present invention can complete the recording of relevant data in the solid phase method experiment process, and the results can be traced back; and reduce the workload of operators.

Description

Technical Field

[0001] The invention relates to the field of biological experiments, in particular to a solid phase method detector and a detection method. Background Art

[0002] Solid phase method, also known as solid phase chemistry or dry chemistry, uses multi-layer thin film solid phase reagent technology. Simply add the liquid sample directly to the reagent carrier that has been solidified in a special structure, that is, the dry chemical reagent, use the water in the sample as the solvent, dissolve the reagent solidified on the carrier, and then react chemically with the components to be tested in the sample, so as to perform analysis and determination, and finally obtain the concentration or activity of the object to be tested.

[0003] At present, solid-phase testing is mainly performed manually in hospitals or physical examination centers, with a low degree of automation and no process control. The experimental results are difficult to trace and have a low degree of standardization. Summary of the invention

[0004] In view of the above problems, the present invention proposes a solid phase method detector and a detection method. The present invention can complete the recording of relevant data during the solid phase method experiment, and the results can be traced back; the workload of operators is reduced.

[0005] The present invention provides the following technical solution: a solid phase method detector, comprising a shell, a sample loading mechanism and a sample loading platform are arranged inside the shell, and the sample loading mechanism is arranged above the sample loading platform, and a card magazine for providing and recovering reagent cards is arranged on one side of the shell; and the card magazine is connected to the shell.

[0006] The loading mechanism is used to transfer the reagent card from the card compartment to the loading platform or to transfer the reagent card from the loading platform to the card compartment to complete the transfer of samples to the dry chemical reagent card; the loading platform is used to place test tubes, reagent cards, reagents, etc.

[0007] The card warehouse includes a reagent card placement rack, a card pushing mechanism is arranged below the reagent card placement rack, a reagent card transfer mechanism is arranged at the exit of the card pushing mechanism, and the reagent card transfer mechanism extends above the sample loading platform. The card pushing mechanism pushes the reagent card to the transfer mechanism, and the transfer mechanism transports the reagent card into the housing, which is convenient for the sample loading mechanism to transport. The transfer mechanism can operate in both directions. After the experiment is completed, the sample loading mechanism transfers the reagent card after the experiment to the transfer mechanism, and the transfer mechanism then transports the reagent card after the experiment out. A photoelectric sensor for counting the reagent cards and a humidity sensor for measuring the humidity in the card warehouse are arranged in the card warehouse.

[0008] The reagent card placement rack and the card pushing mechanism are fixed to the card compartment front cover, the card compartment front cover is connected to the card compartment shell through the card compartment slide rail, and a first electromagnet for adsorbing the card compartment shell is arranged on the top of the card compartment front cover. With the card compartment slide rail, the front cover and the shell form a drawer-like structure, which is convenient for inserting the reagent card, and the electromagnet realizes the fixation of the front cover and the card compartment, so as to prevent the front cover from being pulled out during the use of the detector, which affects the experiment.

[0009] A card discard port is provided on a side of the card bin away from the shell, and the card discard port corresponds to the reagent card transport mechanism. After the experiment is completed, the sample loading mechanism transports the reagent card after the experiment to the transport mechanism, and the transport mechanism puts the reagent card into the card discard port. The staff can set a collection basket at the card discard port to collect the reagent card after the experiment.

[0010] A scanning device for identifying the QR code of the reagent card is arranged above the reagent card transport mechanism, and a positioning device for positioning the reagent card is arranged on the side of the reagent card transport mechanism close to the sample loading platform. In this way, the reagent card can be recorded. After the experiment is over, the reagent card is put back on the transport platform by the gripper, and then the scanning device recognizes and records the QR code on the reagent card again. At the same time, the result of the reagent card after color development is interpreted, and the upper computer of the equipment records the barcode content in the experimental data. The scanning device can use a camera.

[0011] In this scheme, the card bin is a drawer structure, and the card bin can be pulled out to store and load reagent cards. The top of the card bin front cover can be fastened to the frame by an electromagnetic lock, and the bottom is connected to the frame by two tri-fold slide rails. The reagent card placement position can store the reagent cards required for one experiment, and the card pushing structure at the bottom of the placement position can push the reagent card at the bottom of the reagent card placement position to the reagent card transport mechanism during the experiment; the reagent card transport mechanism includes a synchronous belt conveying structure that can rotate left and right and a positioning device. After the card bin pushing mechanism pushes the reagent card to the transport platform, the conveying structure transports the reagent card to the left, that is, in the direction inside the equipment. After it is transported to the designated position, the positioning device will The reagent card is positioned, and then the gripper in the sample loading structure transfers the reagent card to the sample loading platform for experiment; after the experiment, the gripper in the sample loading mechanism grabs the reagent card back to the reagent card transfer mechanism, and the reagent card transfer mechanism transports the reagent card to the right and transfers it to the waste card collection basket outside the equipment; the code scanning device can use a CCD camera, and before the experiment starts, the reagent card is positioned by the positioning structure, and the camera recognizes and records the QR code on the reagent card; after the experiment, the reagent card is put back into the reagent card transfer mechanism by the gripper, and then the camera recognizes and records the QR code on the reagent card again, and at the same time, the result of the reagent card after color development is interpreted.

[0012] The loading platform includes a drawer-type base, which is connected to the shell through a platform slide rail. An inner rotating platform, an outer rotating platform and a fixed platform are arranged on the base. The outer edge of the outer rotating platform clamps the test tube and drives the test tube to rotate, and the test tube is clamped on the periphery of the outer rotating platform. The barcode label on the test tube faces away from the outer rotating platform. A plurality of clamping positions for holding reagent cards are arranged on the inner rotating platform; a disposable suction head placement area for the loading gun and a reagent compartment are arranged on the fixed platform, the inner rotating platform is arranged outside the fixed platform, and the outer rotating platform is arranged outside the inner rotating platform. Both the inner rotating platform and the outer rotating platform are annular, and the fixed platform is circular; the inner rotating platform and the outer rotating platform can rotate in different directions, the central axis of the inner rotating platform and the outer rotating platform coincide, and the fixed platform does not rotate.

[0013] The base is provided with an opening toward the rear of the shell, and a barcode scanner for scanning the barcode label of the test tube is provided in the corresponding opening in the shell, and a second electromagnet for adsorbing and fixing the base is provided in the shell. During the experiment, the reagent or sample in the test tube is placed on the loading platform, the loading position of the test tube is fixed, and the test tube is transferred to the loading position by rotating the external rotating platform; the internal rotating platform of the loading platform drives the reagent card to rotate: the reagent card is transferred from the card compartment to here, the transfer position of the reagent card is fixed, and the loading platform will transfer the reagent card to be transferred to the transfer position by rotation; the disposable suction head used by the loading gun is placed on the fixed platform, and an additional reagent compartment is placed on the fixed platform for other liquids in the experiment; before loading the liquid in the test tube, the device will first rotate the test tube to the barcode scanner, and the barcode on the test tube will be scanned by the barcode scanner, and the barcode content will be recorded in the experimental data by the upper computer of the device. A second electromagnet is placed on the outer shell behind the base. During the experiment, the second electromagnet is used to adsorb the sample loading platform into the outer shell to prevent the sample loading platform from being accidentally pulled out during the experiment. The inner and outer rotating platforms are both controlled by closed loop, that is, an encoder is set on the output shaft of the stepper motor driving the inner and outer rotating platforms. The encoder is connected to the industrial control computer, and the industrial control computer controls the operation of the stepper motor. In this way, feedback of the stepper motor movement can be obtained in time to avoid accidents caused by step loss of the stepper motor driving the inner and outer rotating platforms.

[0014] The loading mechanism comprises an X-direction guide rail, a Y-direction guide rail and a Z-direction guide rail with a driving mechanism, the X-direction guide rail is fixed to the housing, the Y-direction guide rail is installed on the guide slider of the X-direction guide rail through a connecting piece, the Z-direction guide rail is installed on the guide slider of the Y-direction guide rail through a connecting piece, the loading gun is installed on the guide slider of the Z-direction guide rail through an adapter, and the transfer gripper is fixed on the Z-direction guide rail;

[0015] The X-direction guide rail is used for the sample loading gun and the transfer gripper to move in the X-direction; the Y-direction guide rail is used for the sample loading gun and the transfer gripper to move in the Y-direction; the Z-direction guide rail is used for the sample loading gun to move in the Z-direction, and the transfer gripper is fixed together with the Z-direction guide rail.

[0016] The transfer gripper includes a linear motor fixed on a Z-direction guide rail, a suction cup for adsorbing the reagent card is arranged at the front end of the linear motor, an air source for providing negative pressure to the suction cup is arranged in the card compartment, and a vacuum pressure switch is arranged on the pipeline connecting the suction cup and the air source, which can detect whether negative pressure is generated and timely determine whether the reagent card has fallen.

[0017] A detection method of a solid phase detector comprises the following steps:

[0018] S1. Pull out the sample loading platform and the card compartment, and put the samples, reagents and reagent cards required for the experiment into them respectively;

[0019] S2, the card pushing mechanism in the card magazine structure pushes the reagent card to the reagent card transport mechanism. After the reagent card is positioned by the reagent card transport mechanism and the positioning structure, the code scanning device in the card magazine recognizes the QR code on the reagent card and records it in the experimental data. After that, the transport gripper of the loading mechanism transfers the reagent card to the card position on the rotating platform of the loading platform;

[0020] S3, the barcode of the test tube on the loading platform is identified and recorded in the experimental data, and then the loading platform rotates the test tube and the reagent card to the specified position, and the loading mechanism on the top is loaded with a gun head, and the sample / reagent in the test tube and the reagent compartment are respectively aspirated, and the liquid is dispensed at the corresponding position of the reagent card in turn;

[0021] S4. After the reaction time is over, the transfer gripper in the sample loading mechanism transfers the reagent card to the reagent card transfer mechanism in the card compartment. The code scanning device in the card compartment recognizes and enters the QR code on the reagent card, and interprets the result of the color display area of ​​the reagent card. After the interpretation is completed, the reagent card transfer mechanism transports the reagent card to the outside of the device through the discard port.

[0022] From the above description, it can be seen that the detector of this scheme includes: 1. Sample loading part: complete the pipetting of samples to dry chemical reagent cards and the transportation of reagent cards; 2. Platform part: complete the placement of reagent tubes and reagent cards, and complete the scanning of reagent tubes; 3. Card warehouse part: complete the storage of reagent cards, the scanning of reagent cards, and the interpretation of experimental results; the experimental process is: 1. The card warehouse part scans and records the reagent card, and then the sample loading part transfers the reagent card to the platform part; 2. The platform part scans and records the reagent tube, and then the sample loading part absorbs a certain amount of reagent and loads the sample in the reaction area on the reagent card; 3. After a period of reaction time, the sample loading part transfers the reagent card to the interpretation part in the card warehouse part for result interpretation. At the same time, the card warehouse part The reagent card is scanned and recorded again, and finally, the card warehouse part transfers the reagent card to the waste card area, and the experiment ends; in this instrument, the photoelectric sensor, the humidity sensor, the card pushing mechanism, the first electromagnet, the reagent card transfer mechanism, the scanning device, the inner rotating platform, the outer rotating platform, the scanner, the second electromagnet, the X-direction guide rail, the Y-direction guide rail, the Z-direction guide rail, the air source, the vacuum pressure switch, the sample adding gun, the transfer gripper, the display screen, etc. can be connected to the host computer, and the host computer can use an industrial control computer for control work, wherein the photoelectric sensor is used to count the reagent cards in the card warehouse and transmit them to the industrial control computer, and the industrial control computer displays the number of reagent cards on the display screen; the humidity sensor is used to measure the humidity in the card warehouse and transmit the humidity information to the industrial control computer for display on the display screen. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural schematic diagram of a specific implementation mode of the present invention.

[0024] Figure 2 This is a schematic diagram of the detector structure with the right side panel removed.

[0025] Figure 3 This is a schematic diagram of the card warehouse structure without an air source.

[0026] Figure 4 Schematic diagram of the internal structure of the shell.

[0027] Figure 5 Schematic diagram of the sample loading platform structure.

[0028] Figure 6 It is a structural schematic diagram of the sample adding mechanism.

[0029] In the figure, 1 is a shell, 2 is a card compartment, 3 is a display screen, 4 is a front cover of the sample loading platform, 5 is a discard card port, 6 is a reagent card placement rack, 7 is a reagent card transport mechanism, 8 is an air source, 9 is a card pushing mechanism, 10 is a card compartment front cover, 11 is a card compartment slide rail, 12 is a code scanning device, 13 is a first electromagnet, 14 is a sample loading mechanism, 15 is a sample loading platform, 16 is a code scanner, 17 is a test tube compartment, 18 is a card position, 19 is a disposable tip placement area, 20 is a reagent compartment, 21 is a second electromagnet, 22 is an X-direction guide rail, 23 is a Y-direction guide rail, 24 is a Z-direction guide rail, 25 is a transport gripper, 26 is a linear motor, 27 is a suction cup, 28 is a sample loading gun, 29 is an external rotating platform, 30 is an internal rotating platform, 31 is a fixed platform, and 32 is a platform slide rail. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the specific implementation of the present invention. Obviously, the specific implementation described is only one specific implementation of the present invention, not all specific implementations. Based on the specific implementation of the present invention, all other specific implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] As can be seen from the accompanying drawings, the solid phase method detector of the present invention includes a shell 1, in which a sample loading mechanism 14 and a sample loading platform 15 are arranged, and the sample loading mechanism 14 is arranged above the sample loading platform 15, and a card magazine 2 for providing and recovering reagent cards is arranged on one side of the shell 1; and the card magazine 2 is connected to the shell 1, so that the reagent card in the card magazine 2 can enter the sample loading platform 15 for experiment.

[0032] The sample loading mechanism 14 is used to transfer the reagent card from the card compartment 2 to the sample loading platform 15 or to transfer the reagent card from the sample loading platform 15 to the card compartment 2; the sample loading platform 15 is used to place test tubes and reagent cards to complete solid phase method experiments.

[0033] The card warehouse 2 comprises a reagent card placement rack 6, a card pushing mechanism 9 is arranged below the reagent card placement rack 6, a reagent card transport mechanism 7 is arranged at the exit of the card pushing mechanism 9, and the reagent card transport mechanism 7 extends above the sample loading platform 15. A photoelectric sensor for counting reagent cards and a humidity sensor for measuring the humidity in the card warehouse 2 are arranged in the card warehouse 2. The reagent card placement rack 6 and the card pushing mechanism 9 are fixed to the card warehouse front cover 10, and the card warehouse front cover 10 is connected to the housing of the card warehouse 2 through the card warehouse slide rail 11, and a first electromagnet 13 for adsorbing the card warehouse housing is arranged on the top of the card warehouse front cover 10. A discarding port 5 is arranged on the side of the card warehouse 2 away from the shell, and the discarding port 5 corresponds to the reagent card transport mechanism 7. A barcode scanning device 12 for identifying the two-dimensional code of the reagent card is arranged above the reagent card transport mechanism 7. The barcode scanning device 12 interprets the result of the reagent card after color development, and the equipment host computer records the barcode content in the experimental data, which can be displayed through the display screen 3. A positioning device for positioning the reagent card is arranged on the side of the reagent card transport mechanism 7 close to the sample loading platform 15. The positioning device can be an L-shaped card plate for blocking and positioning the reagent card.

[0034] The sample loading platform 15 includes a drawer-type base, and a sample loading platform front cover 4 is arranged at the front end of the base. The sample loading platform front cover 4 is convenient for pulling out the sample loading platform 15. The base is connected to the shell 1 through a platform slide rail 32. An inner rotating platform 30, an outer rotating platform 29 and a fixed platform 31 are arranged on the base. The inner rotating platform 30 is arranged outside the fixed platform 31, and the outer rotating platform 29 is arranged outside the inner rotating platform 30. The outer edge of the outer rotating platform 29 has a test tube compartment 17 for clamping the test tube and driving the test tube to rotate, and the test tube is clamped on the periphery of the outer rotating platform. The barcode label on the test tube Facing the direction away from the outer rotating platform, a plurality of card positions 18 for holding reagent cards are arranged on the inner rotating platform 30; a disposable pipette tip placement area 19 and a reagent compartment 20 are arranged on the fixed platform 31; the base is provided with an opening toward the rear of the outer shell 1; a barcode scanner 16 for scanning the barcode label of the test tube is arranged at the corresponding opening in the outer shell 1; the information scanned by the barcode scanner 16 is recorded in the experimental data by the upper computer of the equipment, and can be displayed on the display screen 3; a second electromagnet 21 for adsorbing the fixed base is arranged in the outer shell 1.

[0035] The loading mechanism 14 includes an X-direction guide rail 22, a Y-direction guide rail 23 and a Z-direction guide rail 24 with a driving mechanism, the X-direction guide rail 22 is fixed to the housing 1, the Y-direction guide rail 23 is installed on the guide slider of the X-direction guide rail 22 through a connecting piece, the Z-direction guide rail 24 is installed on the guide slider of the Y-direction guide rail 23 through a connecting piece, the loading gun 28 is installed on the guide slider of the Z-direction guide rail 24 through an adapter, and the transfer gripper 25 is fixed on the Z-direction guide rail 24 and moves synchronously with the Z-direction guide rail 24; the X-direction guide rail 22 is used for the loading gun 28 and the transfer gripper 25 to move in the X direction; the Y-direction guide rail 23 is used for the loading gun 28 and the transfer gripper 25 to move in the Y direction; the Z-direction guide rail 24 is used for the loading gun 28 to move in the Z direction, and the transfer gripper 25 is fixed to the Z-direction guide rail 24. The transfer gripper 25 includes a linear motor 26 fixed on the Z-direction guide rail 24, a suction cup 27 for adsorbing the reagent card is arranged at the front end of the linear motor 26, an air source 8 for providing negative pressure to the suction cup 27 is arranged in the card bin 2, and a vacuum pressure switch is arranged on the pipeline connecting the suction cup 27 and the air source 8.

[0036] A detection method based on the above-mentioned solid phase detection instrument comprises the following steps:

[0037] S1. Pull out the sample loading platform and the card compartment, and put the samples, reagents and reagent cards required for the experiment into them respectively;

[0038] S2, the card pushing mechanism in the card magazine structure pushes the reagent card to the reagent card transport mechanism. After the reagent card is positioned by the reagent card transport mechanism and the positioning device, the code scanning device in the card magazine identifies the QR code on the reagent card and records it in the experimental data. After that, the transport gripper of the loading mechanism transfers the reagent card to the card position on the rotating platform of the loading platform;

[0039] S3, the barcode of the test tube on the loading platform is identified and recorded in the experimental data, and then the loading platform rotates the test tube and the reagent card to the specified position, and the loading mechanism on the top is loaded with a gun head, and the sample / reagent in the test tube and the reagent compartment are respectively aspirated, and the liquid is dispensed at the corresponding position of the reagent card in turn;

[0040] S4. After the reaction time is over, the transfer gripper in the sample loading mechanism transfers the reagent card to the reagent card transfer mechanism in the card compartment. The code scanning device in the card compartment recognizes and enters the QR code on the reagent card, and interprets the result of the color display area of ​​the reagent card. After the interpretation is completed, the reagent card transfer mechanism transports the reagent card to the outside of the device through the discard port.

[0041] Although specific embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the specific embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A solid phase method detector, characterized in that: The invention comprises a housing (1), a sample loading mechanism (14) and a sample loading platform (15) are arranged inside the housing (1), the sample loading mechanism (14) is arranged above the sample loading platform (15), and a card compartment (2) for providing and recovering a reagent card is arranged on one side of the housing (1); and the card compartment (2) is in communication with the housing (1); The sample loading mechanism (14) is used to transfer the reagent card from the card compartment (2) to the sample loading platform (15) and to transfer the reagent card from the sample loading platform (15) to the card compartment (2), thereby completing the work of transferring the sample to the dry chemical reagent card; The sample loading platform (15) is used to place test tubes and reagent cards; The card bin (2) comprises a reagent card placement rack (6), a card pushing mechanism (9) is arranged below the reagent card placement rack (6), a reagent card transport mechanism (7) is arranged at the exit of the card pushing mechanism (9), and the reagent card transport mechanism (7) extends above the sample loading platform (15); The sample loading platform (15) comprises a drawer-type base, the base being connected to the housing (1) via a platform slide rail (32); an inner rotating platform (30), an outer rotating platform (29) and a fixed platform (31) are arranged on the base; the inner rotating platform (30) is arranged outside the fixed platform (31), and the outer rotating platform (29) is arranged outside the inner rotating platform (30); the outer edge of the outer rotating platform (29) clamps the test tube and drives the test tube to rotate, and the test tube is clamped on the periphery of the outer rotating platform (29), and the barcode label on the test tube faces away from the outer rotating platform (29); a plurality of clamping positions (18) for holding reagent cards are arranged on the inner rotating platform (30); and a disposable pipette tip placement area (19) for a sample loading gun and a reagent compartment (20) are arranged on the fixed platform (31).

2. The solid phase method detector according to claim 1, characterized in that: A photoelectric sensor for counting reagent cards and a humidity sensor for measuring the humidity in the card compartment (2) are arranged in the card compartment (2).

3. The solid phase method detector according to claim 2, characterized in that: The reagent card placement rack (6) and the card pushing mechanism (9) are fixed to the card compartment front cover (10), the card compartment front cover (10) is connected to the shell of the card compartment (2) via a card compartment slide rail (11), and a first electromagnet (13) for adsorbing the card compartment shell is arranged on the top of the card compartment front cover (10).

4. The solid phase method detector according to claim 2, characterized in that: A card discarding port (5) is provided on a side of the card compartment (2) away from the housing (1), and the card discarding port (5) corresponds to the reagent card transport mechanism (7).

5. The solid phase method detector according to claim 2, characterized in that: A code scanning device (12) is arranged above the reagent card transport mechanism (7), and the code scanning device (12) is used to identify the two-dimensional code of the reagent card and to interpret the result of the reagent card after color development; and a positioning device for positioning the reagent card is arranged on a side of the reagent card transport mechanism (7) close to the sample loading platform (15).

6. The solid phase method detector according to claim 1, characterized in that: The base is provided with an opening in a direction toward the rear of the housing (1), a barcode scanner (16) for scanning a barcode label of a test tube is provided in the housing (1) at a position corresponding to the opening, and a second electromagnet (21) for adsorbing and fixing the base is provided in the housing (1).

7. The solid phase method detector according to claim 1, characterized in that: The loading mechanism (14) comprises an X-direction guide rail (22), a Y-direction guide rail (23) and a Z-direction guide rail (24) with a driving mechanism, the X-direction guide rail (22) being fixed to the housing (1), the Y-direction guide rail (23) being mounted on a guide slider of the X-direction guide rail (22) via a connecting piece, the Z-direction guide rail (24) being mounted on a guide slider of the Y-direction guide rail (23) via a connecting piece, the loading gun (28) being mounted on a guide slider of the Z-direction guide rail (24) via an adapter, and the transfer gripper (25) being fixed on the Z-direction guide rail (24); The X-direction guide rail (22) is used for the sample loading gun (28) and the transfer gripper (25) to move in the X-direction; the Y-direction guide rail (23) is used for the sample loading gun (28) and the transfer gripper (25) to move in the Y-direction; the Z-direction guide rail (24) is used for the sample loading gun (28) to move in the Z-direction, and the transfer gripper (25) is fixed to the Z-direction guide rail.

8. The solid phase method detector according to claim 7, characterized in that: The transfer gripper (25) comprises a linear motor (26) fixed on a Z-direction guide rail (24); a suction cup (27) for adsorbing a reagent card is arranged at the front end of the linear motor (26); an air source (8) for providing negative pressure to the suction cup (27) is arranged in the card compartment (2); and a vacuum pressure switch is arranged on a pipeline connecting the suction cup (27) and the air source (8).

9. A detection method based on the solid phase detector according to any one of claims 1 to 8, characterized in that The steps include: S1. Pull out the sample loading platform and the card compartment, and put the samples, reagents and reagent cards required for the experiment into them respectively; S2, the card pushing mechanism in the card magazine structure pushes the reagent card to the reagent card transport mechanism. After the reagent card is positioned by the reagent card transport mechanism and the positioning device, the code scanning device in the card magazine identifies the QR code on the reagent card and records it in the experimental data. After that, the transport gripper of the loading mechanism transfers the reagent card to the card position on the rotating platform of the loading platform; S3, the barcode of the test tube on the loading platform is identified and recorded in the experimental data, and then the loading platform rotates the test tube and the reagent card to the specified position, and the loading mechanism on the top is loaded with a gun head, and the sample / reagent in the test tube and the reagent compartment are respectively aspirated, and the liquid is dispensed at the corresponding position of the reagent card in turn; S4. After the reaction time is over, the gripper in the sample adding mechanism transfers the reagent card to the reagent card transport mechanism in the card compartment. The code scanning device in the card compartment recognizes and enters the QR code on the reagent card, and interprets the result of the color display area of ​​the reagent card. After the interpretation is completed, the reagent card transport mechanism transports the reagent card to the outside of the device through the discard port.

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