Luminescence analysis device and method for adjusting sensitivity of luminescence analysis device

Abstract

The present invention provides a luminescence analysis device and a sensitivity adjustment method thereof that can accurately determine changes in the sensitivity of a photodetector without cost and can miniaturize the device. The luminescence analysis device obtains the concentration of a component to be measured in a sample solution based on the luminescence amount of a measurement solution (4) containing the sample solution and a luminescent reagent that emits light by reacting with the component to be measured in the sample solution and a calibration curve, and is characterized by comprising: a photodetector (20) that detects light from the measurement solution (4); and an arithmetic device (30) that is input with the luminescence amount detected by the photodetector (20). The arithmetic device (30) compares the luminescence amount detected by the photodetector (20) when a blank solution containing no component to be measured is used as the sample solution in the measurement solution 4 with the luminescence amount when the concentration of the component to be measured in the calibration curve is zero, and confirms the sensitivity of the photodetector (20).

Description

Technical Field

[0001] The present invention relates to a luminescence analysis device and a method for adjusting the sensitivity of a luminescence analysis device. More specifically, it relates to a luminescence analysis device applicable to luminescence analysis based on bioluminescence and chemiluminescence and a method for adjusting its sensitivity. Background Art

[0002] Since bioluminescence and chemiluminescence can quantify trace substances in a sample solution, they are used in various fields such as medicine, biochemistry, clinical examination, agriculture, and food-related fields.

[0003] For example, Patent Document 1 discloses a bioluminescence method for highly sensitive detection of endotoxin, in which a reagent activated by endotoxin releases a luminescent substrate from a synthetic substrate and the released luminescent substrate emits light.

[0004] Most of the luminescent reagents used in luminescence analysis, especially those used in luminescence analysis based on bioluminescence, are reagents derived from organisms, and the sensitivity often varies from batch to batch. Therefore, a calibration curve showing the relationship between the component to be measured and the luminescence amount is obtained for each batch of luminescent reagents.

[0005] Prior Art Documents

[0006] Patent Documents

[0007] Patent Document 1: WO 2009 / 063840 Summary of the Invention

[0008] Problems to be Solved by the Invention

[0009] However, the sensitivity of the photodetector used in the luminescence analysis device changes over time, and sometimes the luminescence amount cannot be accurately measured.

[0010] Therefore, it can be considered to provide a reference light source in the device, and by detecting the luminescence amount from this light source using the photodetector, the change in the sensitivity of the photodetector is confirmed, and the measured luminescence amount is corrected.

[0011] However, in this case, since it is necessary to provide a reference light source in the device, not only does the cost increase, but also a space for installing the light source needs to be prepared, making it difficult to miniaturize the device. Moreover, when the luminescence amount of the reference light source itself changes over time, an accurate judgment of the sensitivity of the photodetector cannot be made.

[0012] The present invention has been completed in view of the above circumstances, and an object thereof is to provide a luminescence analysis device and a sensitivity adjustment method thereof that can accurately determine a change in the sensitivity of a photodetector without incurring costs and can achieve miniaturization of the device.

[0013] Technical solutions for solving the problem

[0014] In order to achieve the above object, the present invention adopts the following configuration.

[0015] A first aspect of the present invention is a luminescence analysis device that obtains the concentration of an analyte in a sample solution based on the luminescence amount of a measurement solution containing the sample solution and a luminescent reagent that reacts with the analyte in the sample solution to emit light, and a calibration curve of the luminescent reagent, and is characterized by comprising:

[0016] A photodetector that detects light from the measurement solution; and

[0017] An arithmetic device that is input with the luminescence amount detected by the photodetector,

[0018] The arithmetic device compares the luminescence amount detected by the photodetector when using a blank solution containing no analyte as the sample solution in the measurement solution with the luminescence amount when the concentration of the analyte in the calibration curve is zero, and confirms the sensitivity of the photodetector.

[0019] A second aspect of the present invention is the luminescence analysis device according to the first aspect, and is characterized in that the calibration curve is a calibration curve determined for each batch of the luminescent reagent.

[0020] A third aspect of the present invention is a luminescence analysis device that obtains the concentration of an analyte in a sample solution based on the luminescence amount of a measurement solution containing the sample solution, a luminescent reagent that reacts with the analyte in the sample solution to emit light, and a luminescent substance, and a calibration curve of the luminescent reagent and the luminescent substance, and is characterized by comprising:

[0021] A photodetector that detects light from the measurement solution; and

[0022] An arithmetic device that is input with the luminescence amount detected by the photodetector,

[0023] The arithmetic device compares the luminescence amount detected by the photodetector when using a blank solution containing no analyte as the sample solution in the measurement solution with the luminescence amount when the concentration of the analyte in the calibration curve is zero, and confirms a change in the sensitivity of the photodetector.

[0024] The fourth solution of the present invention is that, for the luminescence analysis device according to the third solution, it is characterized in that the calibration curve is a calibration curve determined for each batch of the luminescence reagent and the luminescence substance.

[0025] The fifth solution of the present invention is that, for the luminescence analysis device according to any one of the first to fourth solutions, it is characterized in that the light detector is a photomultiplier tube.

[0026] The sixth solution of the present invention is a method for adjusting the sensitivity of a luminescence analysis device, which is a method for adjusting the sensitivity of the luminescence analysis device according to the fifth solution, and is characterized in that when the arithmetic device determines that the sensitivity of the light detector has changed and exceeded the specified allowable range, the sensitivity of the light detector is adjusted by changing the voltage applied to the light detector.

[0027] The seventh solution of the present invention is that, for the method for adjusting the sensitivity of a luminescence analysis device according to the sixth solution, it is characterized in that a reagent that emits light through a bioluminescence phenomenon is used as the luminescence reagent.

[0028] Advantages of the Invention

[0029] According to the luminescence analysis device and its sensitivity adjustment method of the present invention, it is not only possible to accurately judge the change in the sensitivity of the light detector without cost, but also to miniaturize the device. Brief Description of the Drawings

[0030] Figure 1 It is a schematic structural diagram of a luminescence analysis device according to an embodiment of the present invention. Detailed Description of the Embodiment

[0031] [First Embodiment]

[0032] Figure 1 It is a luminescence analysis device according to an embodiment of the present invention. The luminescence analysis device of this embodiment includes: a measurement chamber 10 that can store a measurement container 1; a light detector 20 that detects light from inside the measurement container 1 accommodated in the measurement chamber 10; and an arithmetic device 30 that is input with the luminescence amount detected by the light detector 20.

[0033] The measurement chamber 10 is composed of a bottomed cylindrical measurement chamber main body 11 with an opening 11a at the upper end and a door 12 that covers the opening 11a in an openable and closable manner.

[0034] Except for the part of the light-transmitting detection window 11b provided on the side surface, the other parts of the measurement chamber main body 11 are light-shielded. Moreover, the door 12 is also light-shielded.

[0035] When the door 12 is in the open state ( Figure 1When the door 12 is in the state indicated by the dashed line (in the figure), the measurement container 1 can be taken out from / put into the measurement chamber main body 11 through the opening 11a. In addition, when the door 12 is in the closed state ( Figure 1 the state of the door 12 indicated by the solid line in the figure), the measurement chamber 10 can shield external light from entering the interior of the measurement chamber 10.

[0036] As the light detector 20, a photomultiplier tube, a photodiode, a phototransistor, an avalanche photodiode, etc. can be appropriately used.

[0037] In the present embodiment, the light detector 20 detects light only when the door 12 is in the closed state. For example, when the light detector 20 is a photomultiplier tube, a voltage is applied to the light detector 20 only when the door 12 is in the closed state.

[0038] There is no particular limitation on the method of detecting light by the light detector 20 only when the door 12 is in the closed state. For example, it can be a method of using the door 12 itself as a switch and closing the circuit for applying a voltage to the light detector 20 by the top end of the door 12 contacting the periphery of the opening 11a of the measurement chamber main body 11. In addition, a contact sensor that senses the top end of the door 12 contacting the periphery of the opening 11a of the measurement chamber main body 11 can also be used.

[0039] The measurement container 1 accommodated in the measurement chamber 10 is composed of a bottomed cylindrical container body 2 with an upper opening and a lid 3 that liquid-tightly seals the upper end of the container body 2.

[0040] The container body 2 is made of a transparent material such as glass to ensure sufficient light transmission at least in the wavelength range of the light that should be detected by the light detector 20.

[0041] In the first embodiment, the measurement liquid 4 accommodated in the measurement container 1 contains a sample liquid and a luminescent reagent that reacts with the component to be measured in the sample liquid to emit light.

[0042] In the present invention, the luminescent reagent means the entire reagent or reagent group required to generate luminescence corresponding to the concentration of the component to be measured. Usually, a reagent group composed of multiple reagents is equivalent to the luminescent reagent.

[0043] For example, when the component to be measured is a microbial impurity such as endotoxin or β-glucan and bioluminescence is used for analysis, a luminescent reagent containing a reagent activated by the microbial impurity, a luminescent synthesis matrix that releases a luminescent matrix by the activation reagent activated by the microbial impurity, a luminescent enzyme that causes the released luminescent matrix to emit light, and other compounds required for the luminescence reaction is used.

[0044] Next, the luminescent reagent when the component to be measured is endotoxin will be described in detail.

[0045] As a reagent activated by endotoxin, a reagent containing factor C activated by binding to endotoxin is preferred, and a reagent further containing factor B activated by active factor C and pre-prothrombin activated by active factor B and generating coagulase is more preferred. As a reagent containing factor C, factor B, and pre-prothrombin, a component extracted from horseshoe crab blood cells (lysis reagent) can be preferably used.

[0046] As a luminescence synthesis matrix, a luminescence synthesis matrix formed by binding a luminescence matrix and a peptide can be used. As a luminescence synthesis matrix when the component to be measured is endotoxin, a substance having the following structure can be used, that is: a structure in which the binding of the luminescence matrix and the peptide is cleaved by the action (protease activity) of at least any one of active factor C, active factor B, and coagulase.

[0047] As the luminescence matrix, aminofluorescein can be preferably used. As the peptide bound to the luminescence matrix, any one composed of the following amino acid sequence can be used, that is: an amino acid sequence in which the amide bond of aminofluorescein at the C-terminus of the peptide is cleaved by the protease activity of at least any one of active factor C, active factor B, and coagulase.

[0048] The luminescent enzyme is an enzyme that catalyzes the bioluminescence of the luminescence matrix released from the luminescence synthesis matrix to generate light. The luminescent enzyme when the luminescence matrix is aminofluorescein is luciferase, and the other compounds required for the luminescence reaction are ATP and divalent metal ions.

[0049] It should be noted that when the sample solution contains salts, in order to eliminate the error caused by the salt concentration, the luminescence reagent may further contain NaCl.

[0050] Preferably, before injecting the sample solution into the measurement container 1, the luminescence reagent is previously accommodated in the measurement container 1. For example, the luminescence reagent can be attached to the bottom of the container body 2 in a freeze-dried state in advance.

[0051] As long as the luminescence reagent is previously accommodated in the measurement container 1, only by injecting the sample solution into the measurement container 1, it can be used in the analysis using the luminescence analysis device of the present embodiment.

[0052] When an unknown sample with an unknown concentration of the component to be measured is used as the sample solution in the measurement solution 4, the arithmetic device 30 obtains the concentration of the component to be measured in the sample solution based on the luminescence amount detected by the light detector 20.

[0053] Specifically, the concentration of the component to be measured in the sample solution is obtained by comparing the luminescence amount detected by the light detector 20 when the measurement container 1 containing the measurement solution 4 is placed in the measurement chamber 10 with the calibration curve stored in advance.

[0054] The arithmetic device 30 pre-stores a calibration curve showing the relationship between the light emission amount and the concentration of the component to be measured. This calibration curve is determined for each batch of luminescent reagents. When using a new batch of luminescent reagents, it is updated by inputting the information of the calibration curve provided by the manufacturer or the like.

[0055] Preferably, the concentration of the component to be measured obtained can be displayed on a display device built into the luminescence analysis device of the present embodiment or an independent display device, and can be output to a built-in or independent printer and an external computer.

[0056] Preferably, when the arithmetic device 30 determines that the measurement container 1 has been placed in the measurement chamber 10, it immediately performs the operation of obtaining the concentration of the component to be measured in the above-mentioned sample solution.

[0057] Accordingly, only by placing the measurement container 1 in the measurement chamber 10, the arithmetic device 30 can be automatically made to start the operation of obtaining the concentration of the component to be measured in the sample solution.

[0058] For example, when the door 12 is in the open state and then becomes closed, the arithmetic device 30 can determine whether the measurement container 1 has been placed in the measurement chamber 10 based on whether the light detector 20 can detect the background light emission of the luminescent reagent itself.

[0059] Specifically, it is determined whether the background light emission can be detected based on whether the light emission amount detected by the light detector 20 is above a specified threshold value.

[0060] The threshold value is the light emission amount that can clearly determine that the background light emission of the luminescent reagent itself has occurred.

[0061] It should be noted that the measurement container 1 itself may also generate extremely weak natural light emission, but the light emission amount of such natural light emission or a light emission amount close to it should not be used as the threshold value. This is because if the extremely weak light emission amount of the natural light emission from the measurement container 1 itself is used as the threshold value, a light detector 20 with extremely high sensitivity has to be prepared, and it is difficult to accurately judge the presence or absence of background light emission.

[0062] The light emission amount of the background light emission of the luminescent reagent itself can be confirmed by using a sample solution that does not contain the component to be measured as a blank solution and measuring the light emission amount of the measurement solution 4 containing the blank solution and the luminescent reagent.

[0063] The threshold value is preferably 10%-100% of the light emission amount of the background light emission of the luminescent reagent itself, more preferably 30%-70%, and can be about 50% for example.

[0064] When confirming the sensitivity of the photodetector 20, a blank solution that does not contain the component to be measured is used as the sample solution in the measurement solution 4. Moreover, the operator stores the measurement container 1 containing the measurement solution 4 including the blank solution in the measurement chamber 10 and performs an input operation for which the confirmation of sensitivity is required.

[0065] Then, the arithmetic unit 30 compares the amount of light emission detected by the photodetector 20 with the amount of light emission when the concentration of the component to be measured in the calibration curve stored in advance is zero, and confirms the change in the sensitivity of the photodetector 20.

[0066] Here, the amount of light emission when the concentration of the component to be measured in the calibration curve stored in advance is zero (hereinafter sometimes referred to as "zero reference light emission amount") corresponds to the background light emission of the light-emitting reagent itself. For example, the background light emission of the light-emitting reagent itself is generated by the free light-emitting matrix contained in the light-emitting synthesis matrix emitting light due to the light-emitting enzyme.

[0067] The arithmetic unit 30 compares the amount of light emission detected by the photodetector 20 with the zero reference light emission amount. For example, the comparison is made by obtaining the ratio of the amount of light emission detected by the photodetector 20 to the zero reference light emission amount or by obtaining the difference between the two.

[0068] When obtaining the ratio of the amount of light emission detected by the photodetector 20 to the zero reference light emission amount, if the obtained ratio is less than or exceeds the ratio within a preset specified range, the arithmetic unit 30 determines that the sensitivity of the photodetector 20 has exceeded the specified allowable range and has changed.

[0069] In addition, when obtaining the difference between the two, if the obtained difference exceeds the difference within a preset specified range, the arithmetic unit 30 determines that the sensitivity of the photodetector 20 has exceeded the specified allowable range and has changed.

[0070] The specified range can be appropriately set according to the required measurement accuracy, etc. and stored in the arithmetic unit 30.

[0071] When the arithmetic unit 30 confirms that the sensitivity of the photodetector 20 has exceeded the specified allowable range and has changed, the operator can be made to take measures such as adjusting the sensitivity of the photodetector 20 or replacing the photodetector 20 by outputting an alarm or the like.

[0072] If the photodetector 20 is a photomultiplier tube, the sensitivity of the photodetector 20 can be adjusted by changing the voltage applied to the photodetector 20.

[0073] [Second Embodiment]

[0074] The device configuration of the luminescence analysis device according to the second embodiment is the same as that of the first embodiment, as Figure 1It is provided with: a measurement chamber 10 that can store a measurement container 1; a light detector 20 that detects light from inside the measurement container 1 accommodated in the measurement chamber 10; and an arithmetic unit 30 that is input with the amount of light emission detected by the light detector 20.

[0075] In the luminescence analysis device of the second embodiment, the measurement liquid 4 accommodated in the measurement container 1 is different. In addition, corresponding to the difference in the measurement liquid 4, the operation of the arithmetic unit 30 is also different. Other matters are the same as those of the luminescence analysis device of the first embodiment, and thus the description thereof is omitted.

[0076] In the second embodiment, the measurement liquid 4 accommodated in the measurement container 1 contains a sample liquid, a luminescent reagent that emits light by reacting with the component to be measured in the sample liquid, and a luminescent substance.

[0077] The same as the description given in the first embodiment, the luminescent reagent means the entire reagent or reagent group required to generate luminescence corresponding to the concentration of the component to be measured, and a reagent group usually composed of multiple reagents corresponds to the luminescent reagent.

[0078] In the present invention, the luminescent substance is a substance that emits light due to the luminescent reagent in the absence of the component to be measured, or a substance that emits light by itself, and may also be a reagent group composed of multiple reagents.

[0079] The wavelength range of the light emitted by the luminescent substance is preferably equal to, more preferably the same as, the wavelength range of the light emitted when the component to be measured acts on the luminescent reagent. Accordingly, when using a blank solution as the sample liquid, the amount of light emission detected by the light detector 20 can be increased.

[0080] In the absence of the component to be measured, as the substance that emits light due to the luminescent enzyme contained in the luminescent reagent, it may be a luminescent substrate that emits light due to the luminescent enzyme contained in the luminescent reagent. For example, when the luminescent enzyme is firefly luciferase, it is luciferin, etc.

[0081] As the substance that emits light by itself, it may be a combination of other luminescent enzymes different from the luminescent enzyme contained in the luminescent reagent and a luminescent substrate that emits light due to the other luminescent enzyme. For example, a combination of pyrophorus luciferase and luciferin, a combination of Renilla luciferase and coelenterazine, etc.

[0082] When the luminescent substance is a luminescent substrate that emits light due to the luminescent enzyme contained in the luminescent reagent, it is preferably a luminescent substrate that emits light in a wavelength range equal to that of the luminescent substrate released from the luminescent synthetic substrate contained in the luminescent reagent, and particularly preferably the same as the luminescent substrate released from the luminescent synthetic substrate contained in the luminescent reagent.

[0083] For example, as the luminescent reagent, when it contains a luminescent synthetic substrate that liberates aminofluorescein and the luminescent enzyme contains luciferase, aminofluorescein is preferably used as the luminescent substance.

[0084] When the luminescent substance is a combination of a luminescent enzyme different from the luminescent enzyme contained in the luminescent reagent and a luminescent substrate that luminesces due to this other luminescent enzyme, it is preferable that the light emitted by this combination has a wavelength range equal to that of the luminescent substrate liberated from the luminescent synthetic substrate contained in the luminescent reagent.

[0085] For example, as the luminescent reagent, when it contains a luminescent synthetic substrate that liberates aminofluorescein and luciferase is used as the luminescent enzyme, a combination of pyrophyllite luciferase and luciferin is preferably used as the luminescent substance.

[0086] Preferably, before injecting the sample solution into the measurement container 1, the luminescent substance and the luminescent reagent are previously accommodated in the measurement container 1. For example, the luminescent reagent and the luminescent substance can be attached to the bottom of the container body 2 in a freeze-dried state in advance.

[0087] As long as the luminescent reagent and the luminescent substance are previously accommodated in the measurement container 1, only by injecting the sample solution into the measurement container 1, it can be used in the analysis using the luminescence analysis device of this embodiment.

[0088] Similarly, in this embodiment, when an unknown sample with an unknown concentration of the component to be measured is used as the sample solution in the measurement solution 4, the arithmetic unit 30 obtains the concentration of the component to be measured in the sample solution based on the luminescence amount detected by the light detector 20.

[0089] Specifically, the concentration of the component to be measured in the sample solution is obtained by comparing the luminescence amount detected by the light detector 20 when the measurement container 1 containing the measurement solution 4 is placed in the measurement chamber 10 with a calibration curve stored in advance.

[0090] The arithmetic unit 30 stores in advance a calibration curve showing the relationship between the luminescence amount and the concentration of the component to be measured. This calibration curve is determined for each combination of the luminescent reagent and the luminescent substance. When using a new batch of the luminescent reagent and the luminescent substance, it is updated by inputting the information of the calibration curve provided by the manufacturer or the like.

[0091] Preferably, the obtained concentration of the component to be measured can be displayed on a display device built into the luminescence analysis device of this embodiment or an independent display device, and can be output to a built-in or independent printer and an external computer.

[0092] Similarly, in this embodiment, preferably, when the arithmetic unit 30 determines that the measurement container 1 has been placed in the measurement chamber 10, the operation of obtaining the concentration of the component to be measured in the above-mentioned sample solution is performed.

[0093] Accordingly, only by storing the measurement container 1 in the measurement chamber 10, the operation device 30 can be automatically made to start the operation of obtaining the concentration of the component to be measured in the sample solution.

[0094] For example, when the door 12 is in the open state and then changes to the closed state, the operation device 30 can determine whether the measurement container 1 has been stored in the measurement chamber 10 based on whether the light detector 20 can detect the light that combines the background light of the luminescent reagent itself and the light of the luminescent substance.

[0095] The method by which the operation device 30 determines the open / closed state of the door 12 is the same as that in the first embodiment.

[0096] Compared with the case where there is only the background light of the luminescent reagent itself, the amount of light that combines the background light of the luminescent reagent itself and the light of the luminescent substance is larger. Therefore, it is possible to more easily determine whether the measurement container 1 has been stored in the measurement chamber 10.

[0097] Specifically, based on whether the amount of light detected by the light detector 20 is above a specified threshold value, it is determined whether the light that combines the background light and the light of the luminescent substance can be detected.

[0098] The threshold value is the amount of light that can clearly be determined to have generated the light that combines the background light of the luminescent reagent itself and the light of the luminescent substance.

[0099] It should be noted that in this embodiment, similarly, the amount of natural light emitted from the measurement container 1 or an amount of light close to this amount of light should not be used as the threshold value.

[0100] By using a sample solution that does not contain the component to be measured as a blank solution and measuring the amount of light of the measurement solution 4 that contains this blank solution, the luminescent reagent, and the luminescent substance, it is possible to confirm the amount of light that combines the background light of the luminescent reagent itself and the light of the luminescent substance.

[0101] The threshold value is preferably 10% - 100% of the amount of light that combines the background light of the luminescent reagent itself and the light of the luminescent substance, more preferably 30% - 70%, and can be about 50% for example.

[0102] When confirming the sensitivity of the light detector 20, a blank solution that does not contain the component to be measured is used as the sample solution in the measurement solution 4. Moreover, the operator stores the measurement container 1 that contains the measurement solution 4 that contains this blank solution in the measurement chamber 10 and performs the input operation required to confirm the sensitivity.

[0103] Then, the operation device 30 compares the amount of light detected by the light detector 20 with the amount of light when the concentration of the component to be measured in the pre-stored calibration curve is zero, and confirms the change in the sensitivity of the light detector 20.

[0104] Here, the luminescence amount when the concentration of the component to be measured in the pre-stored calibration curve is zero is equivalent to the luminescence that combines the background luminescence of the luminescence reagent itself and the luminescence of the luminescent substance.

[0105] The method of comparison and the method of using the comparison result are the same as those in the first embodiment.

[0106] According to the luminescence analysis device of each of the above embodiments, it is not necessary to provide a light source in the device to accurately judge the sensitivity change of the photodetector 20. Therefore, miniaturization and cost reduction of the device can be achieved.

[0107] Explanation of reference numerals

[0108] 1 Measuring container

[0109] 2 Container main body

[0110] 3 Lid

[0111] 4 Measuring solution

[0112] 10 Measuring chamber

[0113] 11 Measuring chamber main body

[0114] 11a Opening

[0115] 11b Detection window

[0116] 12 Door

[0117] 20 Photodetector

[0118] 30 Arithmetic device.

Claims

1. A luminescence analysis device that obtains the concentration of a component to be measured in a sample solution based on the luminescence amount of a measurement solution containing the sample solution and a luminescent reagent that reacts with the component to be measured in the sample solution and the calibration curve of the luminescent reagent, characterized in that, Comprising: a photodetector that detects light from the assay solution; and an arithmetic unit that is input with the amount of light emission detected by the photodetector, when the assay solution includes the sample solution not containing the analyte and the luminescent reagent, the arithmetic unit compares the amount of light emission detected by the photodetector with the amount of light emission when the concentration of the analyte in the calibration curve is zero, and confirms the sensitivity of the photodetector.

2. The luminescence analysis device according to claim 1, characterized in that, The calibration curve is a calibration curve determined for each batch of the luminescent reagent.

3. The luminescence analysis device according to claim 1 or 2, characterized in that, The photodetector is a photomultiplier tube.

4. A luminescence analysis device that obtains the concentration of a component to be measured in a sample solution based on the luminescence amount of a measurement solution containing the sample solution, a luminescent reagent that reacts with the component to be measured in the sample solution, and a luminescent substance, and the calibration curve of the combination of the luminescent reagent and the luminescent substance, characterized in that, Comprising: a photodetector that detects light from the assay solution; and an arithmetic unit that is input with the amount of light emission detected by the photodetector, when the assay solution includes the sample solution not containing the analyte, the luminescent reagent, and the luminescent substance, the arithmetic unit compares the amount of light emission detected by the photodetector with the amount of light emission when the concentration of the analyte in the calibration curve is zero, and confirms the change in the sensitivity of the photodetector.

5. The luminescence analysis device according to claim 4, characterized in that, The calibration curve is a calibration curve determined for each combination of the luminescent reagent and the luminescent substance in each batch.

6. The luminescence analysis device according to claim 4 or 5, characterized in that, The photodetector is a photomultiplier tube.

7. A method for adjusting the sensitivity of a luminescence analysis device, which is a method for adjusting the sensitivity of the luminescence analysis device according to claim 3, characterized in that, When the arithmetic unit determines that the sensitivity of the photodetector has changed and exceeded the specified allowable range, the sensitivity of the photodetector is adjusted by changing the voltage applied to the photodetector.

8. The method for adjusting the sensitivity of a luminescence analysis device according to claim 7, characterized in that, A reagent that emits light through a bioluminescence phenomenon is used as the luminescent reagent.

9. A method for adjusting the sensitivity of a luminescence analysis device, which is a method for adjusting the sensitivity of the luminescence analysis device according to claim 6, characterized in that, When the arithmetic unit determines that the sensitivity of the photodetector has changed and exceeded the specified allowable range, the sensitivity of the photodetector is adjusted by changing the voltage applied to the photodetector.

10. The method for adjusting the sensitivity of a luminescence analysis device according to claim 9, characterized in that, A reagent that emits light through a bioluminescence phenomenon is used as the luminescent reagent.

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