Biological agent quantitative dispensing device and dispensing method

Abstract

The application relates to the technical field of biological pharmacy, and provides a biological agent quantitative dispensing device and a dispensing method.The biological agent quantitative dispensing device comprises a storage container, a dispensing container, a first non-contact sensor and a second non-contact sensor, the inside of the storage container is used for storing biological agents, the liquid inlet of the dispensing container is communicated with the liquid outlet of the storage container through a conveying pipe, the first non-contact sensor and the second non-contact sensor are arranged on the conveying pipe between the liquid inlet of the dispensing container and the liquid outlet of the storage container, and a preset distance is arranged between the first non-contact sensor and the second non-contact sensor.The non-contact sensor arranged on the conveying pipe between the liquid inlet of the dispensing container and the liquid outlet of the storage container can realize non-contact quantitative dispensing of the biological agents, and can effectively improve the dispensing efficiency and the dispensing precision of the biological agents.

Description

Technical Field

[0001] This invention relates to the field of biopharmaceutical technology, and in particular to a quantitative dispensing device and dispensing method for biological agents. Background Technology

[0002] Biologics repackaging is a crucial component of the biomedical field, vital for cell therapy and pharmaceutical preparation. During repackaging, to prevent contamination, electronic components such as sensors that directly contact the biologics are avoided for quantitative repackaging, making the process quite challenging.

[0003] In related technologies, the main method for dispensing biological agents is by weighing and manually recording the weight. Although this method can achieve quantitative dispensing of biological agents, the dispensing efficiency and accuracy are both low. Summary of the Invention

[0004] This invention provides a quantitative dispensing device for biological agents, which solves at least one technical defect in the prior art, enables contactless quantitative dispensing of biological agents, and can effectively improve the dispensing efficiency and accuracy of biological agents.

[0005] The present invention also provides a method for quantitative dispensing of biological agents.

[0006] To achieve the above objectives, the present invention provides a biological agent quantitative dispensing device, comprising:

[0007] Storage container containing biological agents;

[0008] The dispensing container has its inlet connected to the outlet of the storage container via a delivery pipe.

[0009] A first non-contact sensor and a second non-contact sensor are both disposed on the delivery pipe between the liquid inlet of the dispensing container and the liquid outlet of the storage container, and a preset distance is provided between the first non-contact sensor and the second non-contact sensor.

[0010] According to an embodiment of the present invention, a quantitative dispensing device for biological agents is provided on a delivery tube between the first non-contact sensor and the second non-contact sensor, and a metering device is provided.

[0011] According to an embodiment of the present invention, a biological agent quantitative dispensing device is provided, wherein the dispensing device includes a body, the body having an internal structure with a flow guide tube, the flow guide tube extending along a preset path, the length of the preset path being greater than the distance between the inlet and outlet of the flow guide tube.

[0012] According to an embodiment of the present invention, the biopharmaceutical quantitative dispensing device has a guide tube arranged around the center line of the body and connected to the delivery tube.

[0013] According to the embodiment of the present invention, the cross-sectional area of ​​the guide tube is equal to the cross-sectional area of ​​the delivery tube in the biological agent quantitative dispensing device.

[0014] According to the embodiment of the present invention, in the biological agent quantitative dispensing device, the delivery tube between the first non-contact sensor and the second non-contact sensor is arranged in a spiral shape, which is suitable for extending the flow path of the delivery tube.

[0015] The biological agent quantitative dispensing apparatus provided according to an embodiment of the present invention further includes a pumping component for providing conveying power to the delivery pipe, the pumping component being disposed on any one of the delivery pipe, the storage container, and the dispensing container.

[0016] According to the embodiment of the present invention, a biological agent quantitative dispensing device is provided in which multiple dispensing containers are arranged in parallel, and a switching valve is respectively provided on the delivery pipe between the liquid inlet of each dispensing container and the liquid outlet of the storage container.

[0017] To achieve the above objectives, the present invention provides a method for quantitative dispensing of biological agents, comprising the following steps:

[0018] Acquire the first bubble detection signal of the delivery pipe detected by the first non-contact sensor;

[0019] Acquire the second bubble detection signal of the delivery pipe detected by the second non-contact sensor;

[0020] Calculate the time difference between the first bubble detection signal and the second bubble detection signal;

[0021] The actual volumetric flow rate of the biological agent in the delivery tube is obtained by relating the time difference to the volume or capacity of the delivery tube between the first non-contact sensor and the second non-contact sensor.

[0022] Controlling the flow time of biological agents in delivery tubes to achieve quantitative dispensing of biological agents.

[0023] According to the embodiment of the present invention, the method for quantitative dispensing of biological agents, wherein controlling the flow time of the biological agent in the delivery tube to achieve quantitative dispensing of the biological agent, is to control the opening and closing time of the switching valve at the inlet of each dispensing container under the preset flow rate of the peristaltic pump.

[0024] According to the embodiments of the present invention, a biological agent quantitative dispensing device is provided by setting a first non-contact sensor and a second non-contact sensor on the delivery pipe between the inlet of the dispensing container and the outlet of the storage container. After knowing the volume or capacity of the delivery pipe between the first non-contact sensor and the second non-contact sensor, the volumetric flow rate of the biological agent in the delivery pipe between the first non-contact sensor and the second non-contact sensor can be obtained by detecting and calculating the time difference between the biological agent and the first non-contact sensor when the biological agent flows in the delivery pipe. That is, the actual flow rate in the delivery pipe, thereby controlling the flow time of the biological agent in the delivery pipe to achieve fixed-volume dispensing of the biological agent, that is, to achieve quantitative dispensing of the biological agent. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0026] Figure 1 This is a schematic diagram of the structure of the biological agent quantitative dispensing device provided by the present invention;

[0027] Figure 2 This is a schematic diagram of the metering device in the biological agent quantitative dispensing device provided by the present invention;

[0028] Figure 3 This is a cross-sectional view of the metering device in the biological agent quantitative dispensing apparatus provided by the present invention;

[0029] Figure 4 This is a flowchart of the quantitative dispensing method for biological agents provided by the present invention.

[0030] Figure label:

[0031] 10. Storage container; 20. Dispensing container; 30. First non-contact sensor; 40. Second non-contact sensor; 50. Meter; 51. Body; 52. Flow guide tube; 53. Inlet; 54. Outlet; 60. Delivery tube; 70. Peristaltic pump; 80. Switch valve. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0033] In the description of the embodiments of the present invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of the present invention based on the specific circumstances.

[0034] In embodiments of the present invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0035] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0036] The following is combined with Figures 1 to 4 The embodiments of the present invention will be described below. It should be understood that the following description is merely an illustrative embodiment of the present invention and does not constitute any limitation on the present invention.

[0037] See Figures 1 to 3The first aspect of the present invention provides a biological agent quantitative dispensing device, wherein the biological agent mainly includes cell preparations, as well as biological products processed from microorganisms and their metabolites, effective antigenic components, animal toxins, human or animal blood or tissues, etc., for the prevention, treatment, or diagnosis of corresponding infectious diseases or other related diseases.

[0038] The biological agent quantitative dispensing device includes a storage container 10, a dispensing container 20, a first non-contact sensor 30, and a second non-contact sensor 40. The storage container 10 contains the biological agent to be dispensed. The inlet of the dispensing container 20 is connected to the outlet of the storage container 10 through a delivery pipe 60. The first non-contact sensor 30 and the second non-contact sensor 40 are both installed on the delivery pipe 60 between the inlet of the dispensing container 20 and the outlet of the storage container 10, and a preset distance is provided between the first non-contact sensor 30 and the second non-contact sensor 40.

[0039] The preset distance can be understood as a distance between the first non-contact sensor 30 and the second non-contact sensor 40, which are positioned in the delivery pipe 60. The volume or capacity of the delivery pipe 60 between the first non-contact sensor 30 and the second non-contact sensor 40 is known. Therefore, by detecting and calculating the time difference between the biological agent flowing through the first non-contact sensor 30 and the second non-contact sensor 40 in the delivery pipe 60, and by using the volumetric flow rate relationship between the volume or capacity of the delivery pipe 60 between the first non-contact sensor 30 and the second non-contact sensor 40 and the time difference, the actual volumetric flow rate of the biological agent in the delivery pipe 60 between the first non-contact sensor 30 and the second non-contact sensor 40, i.e., the actual flow rate of the biological agent in the delivery pipe 60, can be achieved by controlling the flow time of the biological agent in the delivery pipe 60.

[0040] It is understood that the present invention, by setting a first non-contact sensor 30 and a second non-contact sensor 40 on the delivery pipe 60 between the liquid inlet of the dispensing container 20 and the liquid outlet of the storage container 10, and after knowing the volume or capacity of the delivery pipe 60 between the first non-contact sensor 30 and the second non-contact sensor 40, can obtain the actual volumetric flow rate of the biological agent in the delivery pipe 60 by detecting and calculating the time difference between the biological agent flowing through the first non-contact sensor 30 and the second non-contact sensor 40 when it flows in the delivery pipe 60. Thus, by controlling the flow time of the biological agent in the delivery pipe 60, the biological agent can be dispensed at a fixed volume, that is, quantitative dispensing of the biological agent can be achieved.

[0041] like Figures 1 to 3As shown, it can be understood that, in order to further improve the dispensing accuracy of the biological agent quantitative dispensing device, based on the above embodiments, the difference is that a metering device 50 is provided on the delivery pipe 60 between the first non-contact sensor 30 and the second non-contact sensor 40. The metering device 50, as an independent component, has a certain structural strength and stability, which facilitates the flow of biological agents inside the metering device 50 and also facilitates detection by the non-contact sensor.

[0042] like Figure 2 and Figure 3 As shown, the metering device 50 can be made of resin material. The structure of the metering device 50 can be set according to actual usage requirements. For example, the shape of the metering device 50 can be a cylinder, cube, cuboid, sphere, etc. When the structure of the metering device 50 is cylindrical, a through hole can be opened in the middle of the cylinder so that the radial cross-section of the cylinder is annular, which facilitates structural design.

[0043] Specifically, the metering device 50 includes a body 51, and the internal structure of the body 51 includes a flow guide tube 52. The flow guide tube 52 extends along a preset path. In order to facilitate the connection between the flow guide tube 52 and the delivery tube 6, an inlet 53 and an outlet 54 are provided on the flow guide tube 52. The length of the preset path is greater than the straight-line distance between the inlet 53 and the outlet 54. It can be understood that the preset path is the path of pipe flow between the inlet 53 and the outlet 54. The preset path can be determined according to the requirements or the shape of the metering device. The preset path is to extend the distance between the inlet 53 and the outlet 54 of the flow guide tube 52 in order to achieve the effect of accurately measuring the actual volumetric flow rate.

[0044] like Figure 3 As shown, to facilitate the processing of the guide tube 52, the guide tube 52 can be arranged around the center line of the body 51. That is, a guide tube 52 is constructed inside the body 51 and arranged around the center line or central axis of the body 51. The guide tube 52 is connected to the delivery tube 60. Since the guide tube 52 is constructed inside the body 51, the guide tube 52 can be understood as a rigid tube and will not deform with the pressure of the flowing medium inside the guide tube 52. Therefore, it can avoid the delivery tube 60 from deforming due to excessive liquid pressure in the delivery tube 60, which would lead to changes in the flow rate of the biological agent in the delivery tube 60 and result in poor quantitative dispensing accuracy of the biological agent.

[0045] like Figure 3 As shown, in order to facilitate accurate calculation of the volume of the guide pipe 52 and the delivery pipe 60, the cross-sectional area of ​​the guide pipe 52 can be set to be equal to the cross-sectional area of ​​the delivery pipe 60.

[0046] Of course, the cross-sectional area of ​​the guide pipe 52 and the cross-sectional area of ​​the delivery pipe 60 can also be set to be different. The specific settings can be selected according to the actual use.

[0047] like Figure 3 As shown, in order to dispense biological agents more accurately, the inlet 53 of the guide tube 52 is located below the body 51, and the outlet 54 of the guide tube 52 is located above the body 51, so that the biological agent in the delivery tube 6 enters from below the metering device 50 and flows out from above the metering device 50, ensuring that the biological agent can fill the entire guide tube 52 and improving the accuracy of the volume or capacity calculation of the guide tube 52.

[0048] Additionally, a connecting section can be provided at the end of the conveying pipe 6. The inner diameter of the channels of inlet 53 and outlet 54 is set to be the same as the inner diameter of the conveying pipe 60, and the inner diameter of the connecting section is set to be larger than the inner diameter of the channels of inlet 53 and outlet 54. Therefore, the conveying pipe 6 can be interference-fitted to the outside of inlet 53 and outlet 54 through the connecting section provided at the end. When calculating the volume or capacity of the conveying pipe 60 between the first non-contact sensor 30 and the second non-contact sensor 40, it can be directly determined by the length and inner diameter of the conveying pipe 60 between the first non-contact sensor 30 and the second non-contact sensor 40.

[0049] In addition, in order to improve the sealing between the inlet 53 and outlet 54 of the delivery pipe 6 and the guide pipe 52, the delivery pipe 6 can be threaded onto the inlet 53 and outlet 54 of the guide pipe 52.

[0050] Once the metering device 50 is manufactured, the volume or capacity of the guide tube 52 is known and fixed. Therefore, when calculating the volume or capacity of the delivery tube 60 between the first non-contact sensor 30 and the second non-contact sensor 40, it is only necessary to calculate the volume or capacity of the delivery tube 60 between the metering device 50 inlet 53 and the first non-contact sensor 30, as well as the volume or capacity of the delivery tube 60 between the metering device 50 outlet 54 and the second non-contact sensor 40, making it more convenient to use.

[0051] In this embodiment, during the quantitative dispensing process, the storage container 10 can be positioned above the dispensing container 20, meaning the height of the outlet of the storage container 10 is greater than the height of the inlet of the dispensing container 20. This allows the biological agent stored in the storage container 10 to automatically enter the delivery pipe 60 under gravity. When the biological agent moves along the delivery pipe 60 and passes the location of the first non-contact sensor 30, the first non-contact sensor 30 detects the biological agent and records the first time of the first bubble detection signal. As the cell preparation continues to move along the delivery pipe 60 and passes the location of the second non-contact sensor 40, the second non-contact sensor 40 detects the biological agent and records the second time of the second bubble detection signal. The difference between the recorded second time and the first time is the time difference between the cell preparation flowing through the delivery pipe 60 and passing the first non-contact sensor 30 and the second non-contact sensor 40.

[0052] Based on the aforementioned time difference and the volume or capacity of the delivery pipe 60 located between the first non-contact sensor 30 and the second non-contact sensor 40 (including the volume or capacity of the guide pipe 52 and the volume or capacity of a portion of the delivery pipe 60), the actual flow rate of the biological agent in the delivery pipe 60 is obtained through the volumetric flow rate calculation formula. Therefore, the actual volume of the biological agent in the dispensing container 20 can be obtained by calculating the time it takes for the biological agent to flow through the first non-contact sensor 30 or the second non-contact sensor 40, thus achieving quantitative dispensing of the biological agent.

[0053] In some embodiments of the present invention, unlike the embodiments described above, in order to avoid the biological agent flowing too fast in the delivery tube 60 and passing through the first non-contact sensor 30 and the second non-contact sensor 40 too quickly, making accurate detection impossible, the delivery tube 60 between the first non-contact sensor 30 and the second non-contact sensor 40 can be arranged in a spiral shape to prolong the flow path of the delivery tube 60 between the first non-contact sensor 30 and the second non-contact sensor 40. This is equivalent to prolonging the time the biological agent flows in the delivery tube 60 between the first non-contact sensor 30 and the second non-contact sensor 40, thereby enabling more accurate detection of the time difference between the biological agent and the first non-contact sensor 30 and the second non-contact sensor 40 as it flows in the delivery tube 60.

[0054] like Figure 1 As shown, as an embodiment of the present invention, based on the above embodiments, the difference is that, in order to make the flow rate in the delivery pipe 60 more stable and uniform, the biological agent quantitative dispensing device further includes a pumping component for providing delivery power to the delivery pipe 60. The pumping component can be installed on any one of the delivery pipe 60, the storage container 10 and the dispensing container 20.

[0055] For example, when the pumping element is a peristaltic pump 70, it's like squeezing a delivery tube 60 filled with biological agent with your finger; as the finger slides forward, the biological agent inside the delivery tube 60 moves forward. In the peristaltic pump 70, rollers replace the finger, pumping the biological agent by alternately squeezing and releasing the delivery tube 60 fixed in the peristaltic pump 70.

[0056] The peristaltic pump 70 can be installed on the delivery pipe 60 between the storage container 10 and the first non-contact sensor 30; the peristaltic pump 70 can also be installed on the delivery pipe 60 between the dispensing container 20 and the second non-contact sensor 40.

[0057] To make the peristaltic pump 70 more precisely controlled, the peristaltic pump 70 is positioned before the inlet 53 of the metering device 50, that is, the peristaltic pump 70 is positioned on the delivery pipe 60 between the storage container 10 and the first non-contact sensor 30.

[0058] For example, when the pumping component is an air pump, the air pump can be installed on the storage container 10 to increase the pressure inside the storage container 10 so that the biological agent in the storage container 10 can enter the delivery pipe 60.

[0059] For example, when the pumping component is a vacuum pump, the vacuum pump can be installed in the dispensing container 20. The vacuum pump evacuates the air inside the dispensing container 20, putting the dispensing container 20 under negative pressure, so that the biological agent in the adsorbed storage container 10 can enter the delivery pipe 60.

[0060] like Figure 1 As shown, as an embodiment of the present invention, unlike the above embodiments, one dispensing container 20 can be set. After one dispensing container 20 is quantitatively dispensed, the next dispensing container 20 can be replaced to continue quantitative dispensing.

[0061] Multiple dispensing containers 20 can be connected in parallel, and each dispensing container 20 has a switching valve 80 installed on the delivery pipe 60 between the liquid inlet of the container 20 and the liquid outlet of the storage container 10.

[0062] That is, the dispensing container 20 is sequentially provided with a first dispensing container, a second dispensing container, a third dispensing container, ... a Nth dispensing container, etc., and a switch valve 80 is provided on the conveying pipe 60 at the inlet of each dispensing container 20, that is, the first dispensing container is equipped with a first switch valve, and the Nth dispensing container is equipped with an Nth switch valve.

[0063] Essentially, the inlet of the first dispensing container and the outlet of the storage container 10 are connected by a main conveying pipe, while the inlets of the second, third, ... Nth dispensing containers are connected by a separate branch conveying pipe, with the switching valve installed on the branch conveying pipe.

[0064] Among them, the switching valve 80 is an electromagnetic pinch valve (referred to as pinch valve), which is driven by an electromagnetic solenoid. It controls the opening and closing of the delivery pipe 60 by squeezing or releasing the delivery pipe 60. The biological agent only passes through the delivery pipe 60, and other parts of the pinch valve do not come into contact with the biological agent, which facilitates contactless quantitative dispensing of the biological agent.

[0065] In this embodiment, after obtaining the actual flow rate of the biological agent in the delivery pipe 60 through the first non-contact sensor 30 and the second non-contact sensor 40, the flow rate of the peristaltic pump 70 can be calibrated to stabilize the flow rate of the biological agent in the delivery pipe 60. Thus, by controlling the opening time of the corresponding switch valve 80 on the dispensing container 20, the actual volume or capacity of the biological agent contained in the dispensing container 20 can be indirectly known, so as to realize the quantitative dispensing of the biological agent.

[0066] In this embodiment of the invention, the first non-contact sensor 30 and the second non-contact sensor 40 are both bubble sensors, used to monitor the bubbles in the delivery pipe 60 in real time, so as to provide feedback on the time it takes for the bubbles in the delivery pipe 60 to pass through.

[0067] The bubble sensor can be installed in a snap-in manner on the delivery tube 60 without cutting it, thus avoiding contamination of the dispensed biological agents and meeting the requirements for contactless dispensing of biological agents. Furthermore, the bubble sensor does not require an external power supply; it can utilize the internal 12V power supply of the peristaltic pump 70, making operation more convenient when used with the pump.

[0068] In this embodiment of the invention, the bubble sensor can be used for detection by infrared detection, capacitance detection and ultrasonic detection. That is, the first non-contact sensor 30 and the second non-contact sensor 40 can be capacitive sensors, ultrasonic sensors, and separate photoelectric sensors.

[0069] In this embodiment of the invention, both the storage container 10 and the dispensing container 20 can be tubular or bag-type containers, such as liquid storage bags, liquid storage tubes, dispensing bags, and dispensing tubes. The delivery tube 60 is a flexible hose, which facilitates the clamping of the peristaltic pump 70 and the non-contact sensor.

[0070] See Figure 1 and Figure 4 A second aspect of the present invention provides a method for quantitative dispensing of biological agents, using the following biological agent quantitative dispensing apparatus:

[0071] Two identical bubble sensors (i.e., the first bubble sensor and the second bubble sensor), a peristaltic pump 70, a metering device 50 (the volume of the metering device 50 is known), several delivery pipes 60, and several dispensing bags are selected, along with several clamp valves corresponding to the dispensing belt. After connecting multiple dispensing bags in parallel, they are connected to the outlet of the storage bag via the delivery pipes 60. The metering device 50 is connected to the delivery pipe 60 between the inlet of the dispensing bag closest to the storage bag and the outlet of the storage bag. The two identical bubble sensors are clamped at both ends of the metering device 50, at the inlet 53 and outlet 54. The peristaltic pump 70 is placed on the delivery pipe 60 between the first bubble sensor and the storage bag. Clamp valves are clamped on each delivery pipe 60 of the dispensing bags.

[0072] It should be noted that before dispensing the biological agent into the storage bag, a peristaltic pump 70 is used to draw air from the dispensing bag and delivery tube 60 into the storage bag to prevent excess gas from occupying the volume of the dispensing bag or delivery tube, thereby affecting the dispensing accuracy of the dispensing bag.

[0073] The main steps of the quantitative dispensing method for biological agents are as follows:

[0074] Step S10: Obtain the first bubble detection signal of the biological agent flowing in the delivery tube 60 detected by the first non-contact sensor 30.

[0075] After calibrating the flow rate of the peristaltic pump 70, the flow rate of the biological agent in the delivery pipe 60 is ensured to be stable. Under the pumping action of the peristaltic pump 70, the biological agent in the storage bag flows from the storage bag into the delivery pipe 60. The biological agent flows along the delivery pipe 60 to the location of the first bubble sensor. The first bubble sensor transmits the detected first bubble detection signal to the controller for storage.

[0076] Step S20: Acquire the second bubble detection signal of the biological agent flowing in the delivery tube 60 detected by the second non-contact sensor 40;

[0077] That is, the biological agent flows along the delivery pipe 60 to pass through the first bubble sensor and enter the metering device 50. After the biological agent fills the guide pipe 52 of the metering device 50, the biological agent flows out of the metering device 50 and enters another delivery pipe 60, and passes through the location of the second bubble sensor. The second bubble sensor transmits the detected second bubble detection signal to the controller for storage.

[0078] Step S30: Calculate the time difference t between the detected first bubble detection signal and the second bubble detection signal;

[0079] That is, by storing the time taken by the controller to pass through the first bubble sensor and the second bubble sensor when the biological agent flows in the delivery tube 60, the time difference t between the biological agent passing through the entire metering device 50 and part of the delivery tube 60 when it flows in the delivery tube 60 is calculated.

[0080] Step S40: By using the relationship between the time difference t and the volume or capacity of the delivery pipe 60 (including the guide pipe 52 and part of the delivery pipe 60) between the first non-contact sensor 30 and the second non-contact sensor 40, the actual flow rate of the biological agent in the delivery pipe 60 is obtained.

[0081] The total volume of the delivery pipe 60 between the first bubble sensor and the outlet 54 of the metering device 50 is obtained by adding the volume of the metering device 50, the volume of the delivery pipe 60 between the first bubble sensor and the outlet 54 of the metering device 50, and the total volume of the delivery pipe 60 between the first bubble sensor and the second bubble sensor. The actual volumetric flow rate Q of the biological agent in the delivery pipe 60 is obtained by dividing the total volume by the time difference t.

[0082] The volumetric flow rate of this invention is expressed by the formula: Qv=V / t=u×A (1-1)

[0083] In the formula: V represents the volume per unit flow rate;

[0084] t represents bit time;

[0085] u represents the average flow velocity inside the pipe;

[0086] A represents the cross-sectional area of ​​the pipe.

[0087] Step S50: Control the flow time of the biological agent in the delivery tube 60 to achieve quantitative dispensing of the biological agent.

[0088] After calibrating the flow rate of the peristaltic pump 70, i.e., determining the flow rate of the biological agent in the delivery pipe 60, the actual volume of the biological agent entering the corresponding dispensing bag is controlled by adjusting the opening and closing time of the clamp valve on each dispensing bag, thereby achieving quantitative dispensing of the biological agent. The dispensing process is carried out sequentially from the first dispensing bag to the Nth dispensing bag.

[0089] In some embodiments of the present invention, in step S50 above, controlling the flow time of the biological agent in the delivery pipe 60 to achieve quantitative dispensing of the biological agent is achieved by controlling the opening and closing time of the switching valve 80 at the inlet of each dispensing container 20 at a preset flow rate of the peristaltic pump 70, which is also the stable flow rate of the peristaltic pump 70 after calibration.

[0090] For example, the volume of the biological agent stored in the storage bag is 304 ml, which needs to be dispensed into 6 dispensing bags. It is known that the volume of the metering device 50 is 5 ml, the flow rate of the peristaltic pump 70 is 24 ml / min, the volume of the delivery tube 60 between the second bubble sensor and the inlet of the sixth dispensing bag is 1.5 ml, and the volume of the branch delivery tube between the inlet of the remaining dispensing bags and the main delivery tube is 0.5 ml.

[0091] When the dispensing of the biological agent begins, the biological agent flows along the delivery tube 60, passes through the first bubble sensor, and enters the metering device 50. After the biological agent fills the guide tube 52 of the metering device 50, it flows out of the metering device 50 and into another delivery tube 60, passing through the location of the second bubble sensor. The second bubble sensor transmits the detected second bubble detection signal to the controller for storage. The time difference between the second bubble detection signal and the first bubble detection signal is calculated to be 12s. The actual flow rate of the biological agent in the delivery tube 60 at this time is calculated using the volumetric flow rate formula as 5ml / 12s = 25ml / min.

[0092] If each dispensing bag has a volume of 50ml, then the time required to dispense the last dispensing bag, i.e., the sixth dispensing bag, is (50+1.5) / 25 = 123.6s, while the time required to dispense the remaining 5 dispensing bags is (50+0.5) / 25 = 121.2s.

[0093] This invention uses two identical bubble sensors to measure the relationship between the time it takes for a biological agent to flow through a metering device 50 and the volume of the metering device 50, calculating the actual inflow rate of the biological agent in the delivery tube 60. The rotation speed of the peristaltic pump 70 is calibrated using the actual inflow rate of the biological agent in the delivery tube 60. Then, under the set flow rate of the peristaltic pump 70, that is, under the stable flow rate of the delivery tube 60, the true volume or capacity of the biological agent in the dispensing bag is determined by controlling the time when the biological agent enters the dispensing bag, so as to achieve contactless dispensing of the biological agent.

[0094] It should be noted that the flow rate of the peristaltic pump 70 is the volume of biological agent that flows through the peristaltic pump 70 per unit time, and the flow rate of the peristaltic pump 70 is directly proportional to its rotational speed.

[0095] This invention uses a combination of two identical bubble sensors and a metering device 50 to measure the actual flow rate of the biological agent in the delivery pipe 60, thereby calibrating the rotation speed of the peristaltic pump 70 and improving the dispensing accuracy and efficiency of the biological agent.

[0096] This invention indirectly measures the actual flow rate in the delivery tube 60 by relating the volume of the biological agent in the delivery tube 60 to the flow time of the biological agent in the delivery tube 60 of a preset length, thereby calibrating the flow rate of the peristaltic pump 70. Given the actual flow rate in the delivery tube 60, quantitative dispensing of the biological agent is achieved by controlling the flow time of the biological agent in the delivery tube 60.

[0097] In addition, the quantitative dispensing method for biological agents provided by this invention can avoid errors caused by vibration or shaking of the quantitative dispensing device during the dispensing process, so as to achieve precise control of the dispensing volume of biological agents. The entire dispensing process has no direct contact with the biological agents, and will not damage the integrity and activity of the biological agents, making it highly practical.

[0098] It should be noted that the technical solutions in the various embodiments of the present invention can be combined with each other, but the basis for such combination is that they can be implemented by those skilled in the art; when the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist, that is, it is not within the protection scope of the present invention.

[0099] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for quantitative dispensing of biological agents, based on a quantitative dispensing device for biological agents, characterized in that, The biological agent quantitative dispensing device includes: Storage container containing biological agents; The dispensing container has its inlet connected to the outlet of the storage container via a delivery pipe. The first non-contact sensor and the second non-contact sensor are both installed on the delivery pipe between the liquid inlet of the dispensing container and the liquid outlet of the storage container, and a preset distance is provided between the first non-contact sensor and the second non-contact sensor. A metering device is provided on the delivery tube between the first non-contact sensor and the second non-contact sensor; the metering device includes a body, the interior of which has a guide tube extending along a preset path, the length of which is greater than the distance between the inlet and outlet of the guide tube; the guide tube is arranged around the centerline of the body and communicates with the delivery tube, the cross-sectional area of ​​the guide tube is equal to that of the delivery tube; the inlet of the guide tube is located below the body, and the outlet of the guide tube is located above the body, so that the biological agent enters from below and flows out from above the metering device, ensuring that the biological agent can fill the entire guide tube; it also includes a pumping component for providing delivery power to the delivery tube; a switching valve is provided on the delivery tube at the liquid inlet of the dispensing container; The dispensing method involves using two bubble sensors to measure the relationship between the time it takes for the biological agent to flow through a metering device and the volume of the metering device to calculate the actual inflow rate of the biological agent in the delivery tube. The speed of the peristaltic pump is calibrated using the actual inflow rate of the biological agent in the delivery tube. Then, under the set flow rate of the peristaltic pump, the true volume or capacity of the biological agent in the dispensing bag is determined by controlling the time it takes for the biological agent to enter the dispensing bag.

2. The method for quantitative dispensing of biological agents according to claim 1, characterized in that, The delivery tube between the first non-contact sensor and the second non-contact sensor is arranged in a spiral shape, which is suitable for extending the flow path of the delivery tube.

3. The method for quantitative dispensing of biological agents according to claim 1, characterized in that, The pumping component is disposed on any one of the delivery pipe, the storage container, and the dispensing container.

4. The method for quantitative dispensing of biological agents according to claim 1, characterized in that, Multiple dispensing containers are connected in parallel, and a switching valve is installed on the delivery pipe between the liquid inlet of each dispensing container and the liquid outlet of the storage container.

5. The method for quantitative dispensing of biological agents according to claim 1 specifically includes the following steps: Acquire the first bubble detection signal of the delivery pipe detected by the first non-contact sensor; Acquire the second bubble detection signal of the delivery pipe detected by the second non-contact sensor; Calculate the time difference between the first bubble detection signal and the second bubble detection signal; The actual volumetric flow rate of the biological agent in the delivery tube is obtained by relating the time difference to the volume or capacity of the delivery tube between the first non-contact sensor and the second non-contact sensor. Controlling the flow time of biological agents in delivery tubes to achieve quantitative dispensing of biological agents.

6. The method for quantitative dispensing of biological agents according to claim 5, characterized in that, The control of the flow time of the biological agent in the delivery tube to achieve quantitative dispensing of the biological agent is achieved by controlling the opening and closing time of the valve at the inlet of each dispensing container under the preset flow rate of the peristaltic pump.

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