Automatic quantitative sampling device and method

Abstract

The present invention relates to the field of mass spectrometry technology, and specifically to an automated quantitative sampling device and method, comprising a support structure, a quantitative needle, a buffer capsule, a liquid level detection sensor, a micro-cleaning pump, a micro-air pump, and a micro-waste liquid pump. The quantitative needle structure is a barb-shaped structure, and its inner cavity volume is a quantitative value, wherein the inner diameter of the barb portion is controlled at 0.3 mm-1 mm, which can effectively control the natural flow of the liquid. The micro-cleaning pump, the micro-air pump, and the micro-waste liquid pump structure form a closed structure, the quantitative needle enters the liquid, and the micro-air pump extracts the gas and liquid in the buffer capsule along the quantitative needle and enters the quantitative needle structure. When the quantitative needle is filled, the overflow portion enters the bottom of the buffer capsule. When the liquid level detection sensor detects the liquid, the quantitative sampling ends and the operation of the micro-air pump is stopped. By optimizing the sampling structure of the quantitative device pipeline, the work efficiency of the operator is effectively improved and the waste of resources is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of mass spectrometry analysis, and in particular to an automated quantitative sampling device and method. Background Art

[0002] With the continuous development of society and the continuous improvement of people's needs, the specifications of laboratory analysis and testing have become more and more standardized, and people's requirements for analysis and testing have become higher and higher. They have higher requirements for the number of samples, analysis cycle, data accuracy, work cost and efficiency in the analysis process. When analytical instruments test liquid samples, they mostly use injectors. Traditional quantitative sampling and detection devices are mostly pipeline sampling structures. However, the operation process of traditional quantitative sampling and detection devices is complicated. It is not easy for operators to absorb samples during operation. Traditional manual sampling and injection operations often cause various errors due to human factors, low work efficiency and waste of resources. Summary of the Invention

[0003] The present invention mainly provides an automated quantitative sampling device and method. The main purpose of the present invention is to solve the problems of complex operation procedures of traditional quantitative sampling detection devices in the prior art, difficulty in aspirating samples during operation by operators, and traditional manual sampling and injection operations often causing various errors due to human factors, low work efficiency and waste of resources.

[0004] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0005] A quantitative sampling device for automated use includes: a supporting structure, a quantitative needle, a buffer capsule, a liquid level detection sensor, a micro-cleaning pump, a micro-air pump, and a micro-waste liquid pump. The supporting structure is provided with a vertical conduit and a transverse conduit, one end of the transverse conduit is fixed to the vertical conduit in a horizontal direction, the transverse conduit is provided with a first hole from the upper wall to the lower wall away from the vertical tube, the quantitative needle is fixed to the transverse tube through the first hole, one end of the quantitative needle is a closed end, and the other end is an open end, the vertical conduit is provided with a second hole and a third hole, the micro-cleaning pump is fixed to the vertical conduit through the second hole by bolts, the micro-air pump is fixed to the vertical conduit through the third hole by bolts, the micro-cleaning pump is fixed to the vertical conduit through the second hole by bolts and is parallel to the micro-cleaning pump and the transverse conduit, the transverse conduit is provided with a fourth hole between the first hole and the vertical conduit, the buffer capsule is fixed to the transverse conduit through the fourth hole, The lower end of the buffer capsule is conical, and the upper end of the buffer capsule is provided with a first connecting pipe and a second connecting pipe, the first connecting pipe is connected to the micro-cleaning pump, and the second connecting pipe is connected to the second connecting pipe, the quantitative needle is facing the buffer capsule and the buffer capsule is facing the quantitative needle. The hole is respectively provided, and the pipe is connected obliquely downward from the hole facing the buffer capsule with the hole facing the quantitative needle. The conical end of the buffer capsule is connected to the liquid level detection sensor, and the lower end of the liquid level detection sensor is provided with a third connecting pipe, and the third connecting pipe is connected to the liquid level detection sensor. The third connecting pipe extends vertically downward, and then extends horizontally from the direction of the liquid level detection sensor to the direction of the vertical pipe. The third connecting pipe extends vertically upward away from one end of the liquid level detection sensor to connect to the micro waste liquid pump. The direction of the micro waste liquid pump connected to the third connecting pipe is an open end, and the direction away from the third connecting pipe is a closed end.

[0006] Furthermore, the opening end of the quantitative needle is a bevel incision, and the inner cavity of the quantitative needle is provided with a U-shaped catheter to control the natural flow of liquid.

[0007] Furthermore, an air guide groove is provided on the outer wall of the quantitative needle for balancing the pressure inside the bottle and the atmosphere after puncture.

[0008] Furthermore, the inner diameter of the quantitative needle cavity is set to 0.3mm-1mm, which can effectively control the natural flow of the liquid.

[0009] Furthermore, the first conduit extends vertically upward to form a first extension section, the first extension section extends horizontally toward the vertical tube to form a second extension section, the second extension section extends vertically downward to form a third extension section, the third extension section passes through the upper wall of the micro-cleaning pump close to the first conduit and is fixedly connected to the vertical conduit, and the end of the third extension section is conical, the second conduit extends vertically upward to form a first extension section, the first extension section extends horizontally toward the vertical tube to form a second extension section, the second extension section extends vertically downward to form a third extension section, the third extension section passes through the upper wall of the micro-air pump close to the first conduit and is fixedly connected to the vertical conduit, and the end of the third extension section is conical.

[0010] Furthermore, the quantitative device structure is used to quantify 100uL-2mL of liquid, and the volume of the buffer capsule 3 is 100uL;

[0011] Furthermore, the flow rate of the micro air pump is 10 mL / min;

[0012] Furthermore, the flow rate of the micro waste liquid pump is 30 mL / min;

[0013] Furthermore, the quantitative device structure is used to quantitatively measure 100uL-2mL of liquid.

[0014] An automated quantitative sampling method comprising:

[0015] The micro cleaning pump, micro air pump, and micro waste liquid pump structure form a closed structure, and the quantitative needle enters the liquid;

[0016] The micro air pump draws the gas and liquid in the buffer bag along the quantitative needle into the quantitative needle structure;

[0017] When the quantitative needle is filled, the overflow portion enters the bottom of the buffer bag;

[0018] When the liquid level detection sensor detects liquid, the quantitative injection is completed and the operation of the micro air pump is stopped.

[0019] Furthermore, the quantitative needle is cleaned, the operation of the micro waste liquid pump is stopped, and the micro cleaning pump is used to perfuse cleaning liquid into the buffer bag and the quantitative needle to clean the quantitative needle.

[0020] Clean the buffer capsule. When the quantitative needle is cleaned, open the micro waste liquid pump to discharge waste, and simultaneously start the micro cleaning pump to clean;

[0021] Determining whether the flow rate of the micro waste liquid pump is more than twice the flow rate of the micro cleaning pump;

[0022] When the flow rate of the micro waste liquid pump is more than twice the flow rate of the micro cleaning pump, the operation of the micro waste liquid pump is stopped, and the micro air pump is started to reverse to remove the residual cleaning liquid;

[0023] Complete the cleaning work.

[0024] The main technical principle of this structure is that the quantitative needle structure is a barbed hook structure, and its inner cavity volume is a quantitative value. The inner diameter of the barb part is controlled at 0.3mm-1mm, which can effectively control the natural flow of liquid. The micro cleaning pump, micro air pump, and micro waste liquid pump structure form a closed structure. The quantitative needle enters the liquid, and the micro air pump draws the gas and liquid in the buffer capsule along the quantitative needle into the quantitative needle structure. When the quantitative needle is filled, the overflow enters the bottom of the buffer capsule. When the liquid level detection sensor detects liquid, the quantitative injection ends and the micro air pump stops working.

[0025] Beneficial effect: By optimizing the sampling structure of the quantitative device pipeline, the operator's work efficiency is effectively improved and resource waste is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Schematic diagram of the structure of a quantitative sampling device for automated use;

[0027] Figure 2 A schematic diagram of the planar structure of a quantitative sampling device for automated use;

[0028] Figure 3 Flow chart of the method of using the quantitative injection device for automated use;

[0029] Figure 4 Flow chart of the cleaning method for the quantitative injection device for automated use.

[0030] Figure numerals: 1. Support structure; 2. Quantitative needle; 3. Buffer capsule; 4. Liquid level detection sensor; 5. Micro cleaning pump; 6. Micro air pump; 7. Micro waste liquid pump. DETAILED DESCRIPTION

[0031] The following is a detailed description of an automated quantitative sampling device and method according to the present invention in conjunction with embodiments.

[0032] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be internal communication between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. In the accompanying drawings, the same or similar numbers from beginning to end represent the same or similar elements or elements with the same or similar functions. The described embodiments are part of the embodiments of the present invention, not all of the embodiments. In the absence of conflict, the embodiments in this application and the features in the embodiments can be combined with each other. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0033] refer to Figure 1 and Figure 2, an automated quantitative sampling device includes a supporting structure 1, a quantitative needle 2, a buffer capsule 3, a liquid level detection sensor 4, a micro-cleaning pump 5, a micro-air pump 6, and a micro-waste liquid pump 7. The supporting structure 1 is provided with a vertical conduit and a transverse conduit, one end of the transverse conduit is fixed to the vertical conduit in the horizontal direction, and the transverse conduit is provided with a first hole from the upper wall to the lower wall away from the vertical tube, the quantitative needle 2 is fixed to the transverse tube through the first hole, one end of the quantitative needle 2 is a closed end, and the other end is an open end, the vertical conduit is provided with a second hole and a third hole, the micro-cleaning pump 5 is fixed to the vertical conduit through the second hole by bolts, the micro-air pump 6 is fixed to the vertical conduit through the third hole by bolts, the micro-cleaning pump 5 and the transverse conduit are fixed to the vertical conduit through the second hole by bolts, and the transverse conduit is provided with a fourth hole between the first hole and the vertical conduit, the buffer capsule 3 is fixed to the transverse tube through the fourth hole, The lower end of the buffer capsule 3 is conical, and the upper end of the buffer capsule 3 is provided with a first connecting pipe and a second connecting pipe. The first connecting pipe is connected to the micro-cleaning pump 5, and the second connecting pipe is connected to the second connecting pipe. The quantitative needle 2 is provided with holes facing the buffer capsule 3 and the buffer capsule 3 is provided with holes facing the quantitative needle 2. The pipe is connected obliquely downward from the hole of the quantitative needle 2 facing the buffer capsule 3 to the hole of the buffer capsule 3 facing the quantitative needle 2. The conical end of the buffer capsule 3 is connected to the liquid level detection sensor 4. The lower end of the liquid level detection sensor 4 is provided with a third connecting pipe. The third connecting pipe is connected to the liquid level detection sensor 4. The third connecting pipe extends vertically downward and then extends horizontally from the direction of the liquid level detection sensor 4 to the direction of the vertical pipe. The third connecting pipe extends vertically upward away from the end of the liquid level detection sensor 4 to connect to the micro-waste liquid pump 7. The direction of the micro-waste liquid pump 7 connected to the third connecting pipe is an open end, and the direction away from the third connecting pipe is a closed end.

[0034] In one possible embodiment, the open end of the quantitative needle 2 is a bevel incision, the inner cavity of the quantitative needle 2 is provided with a U-shaped catheter to control the natural flow of liquid, and the outer wall of the quantitative needle 2 is provided with an air guide groove for balancing the pressure in the bottle and the atmosphere after puncture, making it easier to absorb the sample. When a quantitative sample needs to be injected, the micro air pump 6 can be reversed. At this time, the sample injected is only the sample in the quantitative needle, meeting the quantitative requirements.

[0035] The inner diameter of the inner cavity of the quantitative needle 2 is set to 0.3mm-1mm.

[0036] In one possible embodiment, the first conduit extends vertically upward to form a first extension section, the first extension section extends horizontally toward the vertical tube to form a second extension section, the second extension section extends vertically downward to form a third extension section, the third extension section passes through the upper wall of the micro-cleaning pump 5 close to the first conduit of the vertical conduit and is fixedly connected, and the end of the third extension section is conical, the second conduit extends vertically upward to form a first extension section, the first extension section extends horizontally toward the vertical tube to form a second extension section, the second extension section extends vertically downward to form a third extension section, the third extension section passes through the upper wall of the micro-air pump 6 close to the first conduit of the vertical conduit and is fixedly connected, and the end of the third extension section is conical.

[0037] In one possible embodiment, the quantitative device structure is used to quantify 100uL-2mL of liquid, the volume of the buffer capsule 3 is 100uL, the flow rate of the micro air pump 6 is 10mL / min, and the flow rate of the micro waste liquid pump 7 is 30mL / min.

[0038] See also Figure 3 , a quantitative sampling method for automated use,

[0039] S100: The micro-cleaning pump, micro-air pump, and micro-waste pump form a closed structure, and the quantitative needle enters the liquid;

[0040] S200: the micro air pump extracts the gas and liquid in the buffer bag along the quantitative needle and enters the quantitative needle structure;

[0041] S300: When the quantitative needle is filled, the overflowed portion enters the bottom of the buffer bag;

[0042] S400 When the liquid level detection sensor detects liquid, the quantitative injection is completed and the operation of the micro air pump is stopped.

[0043] See also Figure 4 , the cleaning method of the device is,

[0044] S500: cleaning the quantitative needle, stopping the operation of the micro waste liquid pump, and using the micro cleaning pump to perfuse cleaning liquid into the buffer bag and the quantitative needle to clean the quantitative needle;

[0045] S600 cleans the buffer capsule. When the quantitative needle is cleaned, the micro waste liquid pump is turned on to discharge waste, and the micro cleaning pump is simultaneously started to clean;

[0046] S700 determines whether the flow rate of the micro waste liquid pump is more than twice the flow rate of the micro cleaning pump. This step can prevent the cleaning liquid from being discharged from the quantitative needle at this time.

[0047] S800: When the flow rate of the micro waste liquid pump is more than twice the flow rate of the micro cleaning pump, the operation of the micro waste liquid pump is stopped, and the micro air pump is started to reverse to remove the residual cleaning liquid;

[0048] S900 completes the cleaning process.

[0049] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An automated quantitative sampling device, characterized in that: The pump is mounted on a pneumatic tube and has a top end mounted on a pneumatic tube, and the pneumatic tube has a bottom end and a bottom end mounted on a pneumatic tube. The pump is mounted on a pneumatic tube and has a bottom end. The pneumatic tube is mounted on a pneumatic tube and has a bottom end. The pneumatic tube is mounted on a pneumatic tube and has a bottom end. Two connecting pipes are connected to the micro air pump, and the quantitative needle is respectively provided with holes in the direction of the buffer capsule and the buffer capsule is facing the quantitative needle. The pipe is inclined downward from the hole in the direction of the quantitative needle facing the buffer capsule to connect to the hole in the direction of the quantitative needle in the buffer capsule, and the pipe makes the quantitative needle form a barb-type structure; the inner diameter of the barb-type structure is set to 0.3mm-1mm to control the natural flow of the liquid; the tapered end of the buffer capsule is connected to the liquid level detection sensor, and the lower end of the liquid level detection sensor is provided with a third connecting pipe, and the third connecting pipe is connected to the liquid level detection sensor. The third connecting pipe extends vertically downward, and then extends horizontally from the direction of the liquid level detection sensor to the direction of the vertical tube. The third connecting pipe extends vertically upward away from the end of the liquid level detection sensor to connect to the micro waste liquid pump, the direction of the micro waste liquid pump connected to the third connecting pipe is an open end, and the direction away from the third connecting pipe is a closed end; the open end of the quantitative needle is a bevel incision; the outer wall of the quantitative needle is provided with an air guide groove, which is used to balance the pressure in the bottle and the atmosphere after puncture.

2. The automated quantitative sampling device according to claim 1, characterized in that: The first connecting pipe extends vertically upward to form a first extension section, the first extension section extends horizontally toward the vertical pipe to form a second extension section, the second extension section extends vertically downward to form a third extension section, the third extension section passes through the upper wall of the micro-cleaning pump close to the first connecting pipe of the vertical pipe and is fixedly connected, and the end of the third extension section is conical, the second connecting pipe extends vertically upward to form a first extension section, the first extension section extends horizontally toward the vertical pipe to form a second extension section, the second extension section extends vertically downward to form a third extension section, the third extension section passes through the upper wall of the micro-air pump close to the first connecting pipe of the vertical pipe and is fixedly connected, and the end of the third extension section is conical.

3. The automated quantitative sampling device according to claim 1, characterized in that: Including, the quantitative needle is used to quantify 100uL-2mL of liquid, and the volume of the buffer capsule is 100uL.

4. The automated quantitative sampling device according to claim 1, characterized in that: Including, the flow rate of the micro air pump is 10mL / min.

5. The automated quantitative sampling device according to claim 1, characterized in that: Including, the flow rate of the micro waste liquid pump is 30mL / min.

6. A method for automated quantitative sampling, characterized in that: comprising a quantitative sampling device for automated use according to any one of claims 1 to 5, The micro cleaning pump, micro air pump, and micro waste liquid pump structure form a closed structure, and the quantitative needle enters the liquid; The micro air pump draws the gas and liquid in the buffer bag along the quantitative needle into the quantitative needle structure; When the quantitative needle is filled, the overflow portion enters the bottom of the buffer bag; When the liquid level detection sensor detects liquid, the quantitative injection is completed and the operation of the micro air pump is stopped.

7. The automated quantitative sampling method according to claim 6, wherein: Also includes, Cleaning the quantitative needle, stopping the operation of the micro waste liquid pump, and using the micro cleaning pump to perfuse cleaning liquid into the buffer bag and the quantitative needle to achieve cleaning of the quantitative needle; Clean the buffer capsule. After cleaning the quantitative needle, open the micro waste liquid pump to discharge waste, and simultaneously start the micro cleaning pump to clean; Determining whether the flow rate of the micro waste liquid pump is more than twice the flow rate of the micro cleaning pump; When the flow rate of the micro waste liquid pump is more than twice the flow rate of the micro cleaning pump, the operation of the micro waste liquid pump is stopped, and the micro air pump is started to reverse to remove the residual cleaning liquid; Complete the cleaning work.

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