A microwell plate pipetting device

Abstract

The application discloses a microwell plate pipetting device, which comprises a box body, a microwell plate arranged at the bottom end of the inside of the box body, culture solution boxes arranged on both sides of the microwell plate, and a reciprocating pipetting mechanism arranged at the top of the box body, wherein the reciprocating pipetting mechanism is used for pipetting and adding culture solutions in the culture solution boxes on both sides into microwells on the microwell plate, an opening end of the box body is provided with a sealing door, and one side of the outer wall of the box body is provided with a controller; the reciprocating pipetting mechanism can pipette and add different cell culture solutions in the two culture solution boxes into the microwells on the microwell plate at the same time, and is used in cooperation with a driving mechanism; after one-time pipetting and adding is completed, the driving mechanism drives the microwell plate to move one station backward, and then pipetting and adding is performed again, until all the microwells on the microwell plate are completely pipetted and added, so that the working efficiency of pipetting and adding is greatly improved.

Description

Technical Field

[0001] This invention relates to the field of biochemical analysis technology, specifically to a microplate pipetting device. Background Technology

[0002] Microplates of various sizes are widely used in laboratories as carriers for various experimental purposes such as sample collection for analytical instruments and cell culture. During experiments, it is necessary to add culture medium to the cell culture in the wells of the microplate. The most common pipetting method is to use a pipetting arm to dispense a small amount of liquid onto the microplate placed on an automated pipetting platform. However, in the existing technology, when adding two different culture media to the same microplate at the same time, the addition of one culture media must be completed before the addition of the other culture media can continue, resulting in low pipetting efficiency. Therefore, it is necessary to design a microplate pipetting device to improve the above problems. Summary of the Invention

[0003] 1. The technical problem that the invention aims to solve

[0004] To address the problem of low efficiency in pipetting in existing technologies, this invention designs a microplate pipetting device. Through the designed reciprocating pipetting mechanism, different cell culture media from two culture media boxes can be simultaneously pipetted into the microwells of the microplate, greatly improving the efficiency of pipetting.

[0005] 2. Technical Solution

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A microplate pipetting device includes a housing, a microplate disposed at the bottom of the housing, culture medium boxes disposed on both sides of the microplate, and a reciprocating pipetting mechanism disposed on the top of the housing. The reciprocating pipetting mechanism is used to pipe culture medium from the culture medium boxes on both sides into the microwells of the microplate. The housing has a sealing door at the opening end and a controller is disposed on one side of the outer wall of the housing.

[0008] The bottom of the microporous plate is provided with a movable plate, the top of the movable plate is provided with a microporous plate limiting frame, the microporous plate is placed in the microporous plate limiting frame, and the inside of the box is provided with a driving mechanism that drives the movable plate to move longitudinally.

[0009] The bottom of the culture medium box is provided with a receiving plate, and the top of the receiving plate is provided with a culture medium box limiting frame, and the culture medium box is placed inside the culture medium box limiting frame;

[0010] The reciprocating pipetting mechanism includes a lifting plate, a first motor, a driving toothed pulley, a rotating shaft, a driven toothed pulley, a toothed belt, pipetting tip modules, and a driving component. The first motor is installed at one end of the bottom of the lifting plate, and the output end of the first motor is fixedly connected to the driving toothed pulley. The rotating shaft is rotatably connected to the other end of the bottom of the lifting plate, and the driven toothed pulley is fixedly connected to the bottom end of the rotating shaft. A toothed belt drives between the driving toothed pulley and the driven toothed pulley. Two sets of pipetting tip modules are fixed at the bottom end of the toothed belt, and the two sets of pipetting tip modules are respectively fixed on toothed belt segments with opposite transmission directions. A driving component is provided between the top center of the lifting plate and the housing, and stabilizing components are provided between the top two sides of the lifting plate and the housing.

[0011] Preferably, the drive mechanism includes a second motor, a lead screw, a bearing seat, and a threaded sleeve. The second motor is installed on the rear side of the bottom of the housing. The output end of the second motor is fixedly connected to the lead screw, which is arranged longitudinally. Both ends of the lead screw are fixed to the housing through the bearing seat. The threaded sleeve is threaded onto the lead screw and is installed at the bottom of the movable plate.

[0012] Preferably, the lead screw is symmetrically provided with first slide rails on both sides, the first slide rails are installed at the bottom of the housing, and a first slider is slidably connected on the first slide rails, the first slider being installed at the bottom of the movable plate.

[0013] Preferably, the stabilizing component includes a fixed plate and a telescopic plate. The fixed plate has a cavity inside, and limit grooves are vertically provided on both sides of the cavity. The telescopic plate is placed inside the cavity of the fixed plate, and limit blocks that are slidably connected to the limit grooves are provided on both sides of its outer wall. The top of the fixed plate is fixed to the top of the box body, and the bottom of the telescopic plate is fixed to the top of the lifting plate.

[0014] Preferably, the microporous plate limiting frame has through slots on both sides, and the movable plate has grooves corresponding to the through slots.

[0015] Preferably, a second slide rail is longitudinally arranged on both sides below the receiving plate. The second slide rail is installed at the bottom of the box. A second slider is slidably connected to the second slide rail and is installed at the bottom of the receiving plate.

[0016] Preferably, the receiving plate has a receiving cavity inside, and the top of the receiving plate is provided with a plurality of positioning members within the limiting frame of the culture medium box. The positioning members pass through the top of the receiving plate and communicate with the receiving cavity. A liquid collection box is provided inside the receiving cavity, and a handle is provided on the outer wall of the liquid collection box.

[0017] Preferably, a positioning plate is provided on the rear side of the receiving plate, the positioning plate is fixed to the bottom of the box, and the receiving plate and the positioning plate are tightly fitted together.

[0018] 3. Beneficial effects

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] (1) The microplate pipetting device in this embodiment of the invention can simultaneously pipette different cell culture media from two culture media into the microwells of the microplate through the reciprocating pipetting mechanism. It is used in conjunction with the driving mechanism. After one pipetting operation is completed, the driving mechanism drives the microplate to move backward one position and then pipette again until all microwells on the microplate are pipetted, which greatly improves the efficiency of pipetting.

[0021] (2) In the microplate pipetting device of this embodiment, through grooves are provided on both sides of the microplate limiting frame, and grooves are provided on the moving plate corresponding to the through grooves. When removing the microplate, the operator inserts his finger into the groove, and the finger passes through the through groove to lift the microplate upward, which facilitates the removal and replacement of the microplate.

[0022] (3) In the microplate pipetting device of the present invention, when the microplate pipetting device is not working, the culture medium box on the receiving plate is taken out by the positioning member, and then the two sets of pipetting tip modules are moved to the top of the receiving plate so that the pipetting tip is vertically aligned with the positioning member. The lifting plate and the pipetting tip module are driven to descend by the driving member to store the pipetting tip in the positioning member, so as to avoid contamination of the pipetting tip. The culture medium remaining on the pipetting tip flows into the collection box for collection, which will not cause contamination to the inside of the box and avoid the possibility of cross-contamination. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the overall structure of a microplate pipetting device according to the present invention;

[0024] Figure 2 This is an internal top view of a microplate pipetting device according to the present invention;

[0025] Figure 3 This is a schematic diagram of the reciprocating pipetting mechanism of a microplate pipetting device according to the present invention;

[0026] Figure 4 This is a schematic diagram of the stabilizing component structure of a microplate pipetting device according to the present invention;

[0027] Figure 5 This is a schematic diagram of the moving plate and microplate limiting frame structure of a microplate pipetting device according to the present invention;

[0028] Figure 6 This is a schematic diagram of the receiving plate structure of a microplate pipetting device according to the present invention;

[0029] Figure 7This is a cross-sectional view of the positioning element of a microplate pipetting device according to the present invention.

[0030] In the diagram: 1. Housing; 2. Sealed door; 3. Controller; 4. Moving plate; 41. Groove; 5. Microplate limiting frame; 51. Through groove; 6. Microplate; 7. Reciprocating pipetting mechanism; 71. Lifting plate; 72. First motor; 73. Driving toothed pulley; 74. Rotating shaft; 75. Driven toothed pulley; 76. Toothed belt; 77. Pipette tip module; 78. Drive component; 79. Stabilizing component; 791. Fixing plate; 792. Telescopic plate; 793. Limiting groove; 794. Limiting block; 8. Receiving plate; 81. Receiving cavity; 82. Positioning component; 83. Liquid collection box; 84. Handle; 9. Culture medium box limiting frame; 10. Culture medium box; 11. Threaded sleeve; 12. First slide rail; 13. First slider; 14. Second motor; 15. Lead screw; 16. Bearing seat; 17. Second slide rail; 18. Second slider; 19. Positioning plate. Detailed Implementation

[0031] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0032] To facilitate understanding of the present invention, a more complete description will be given below with reference to the accompanying drawings. Several embodiments of the invention are illustrated in the drawings. However, the invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

[0033] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or there may be an intervening component. When a component is said to be "connected to" another component, it can be directly connected to the other component or there may be an intervening component. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the specification of this invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0035] Example:

[0036] This invention provides a microplate pipetting device for pipetting cell culture media into microplates. The device can simultaneously pipette different cell culture media from two culture media boxes into the microwells of the microplate, greatly improving the efficiency of pipetting.

[0037] Please see Figure 1-7 A microplate pipetting device includes a housing 1, a microplate 6 disposed at the bottom of the housing 1, culture medium boxes 10 disposed on both sides of the microplate 6, and a reciprocating pipetting mechanism 7 disposed on the top of the housing 1. The reciprocating pipetting mechanism 7 is used to pipette culture medium from the culture medium boxes 10 on both sides into the microwells of the microplate 6. A sealing door 2 is provided at the opening end of the housing 1, and a controller 3 is provided on one side of the outer wall of the housing 1. When in use, the microplate pipetting device can simultaneously pipette different cell culture media from the two culture medium boxes 10 into the microwells of the microplate 6 through the reciprocating pipetting mechanism 7, which greatly improves the efficiency of pipetting.

[0038] Reference Figure 1 , Figure 2 and Figure 5 As shown, a movable plate 4 is provided at the bottom of the microporous plate 6, and a microporous plate limiting frame 5 is provided at the top of the movable plate 4. The microporous plate 6 is placed inside the microporous plate limiting frame 5, and a driving mechanism that drives the movable plate 4 to move longitudinally is provided inside the housing 1.

[0039] Among them, reference Figure 1 , Figure 2 and Figure 6 As shown, a receiving plate 8 is provided at the bottom of the culture medium box 10, and a culture medium box limiting frame 9 is provided at the top of the receiving plate 8. The culture medium box 10 is placed inside the culture medium box limiting frame 9, and the position of the culture medium box 10 corresponds to the position of the pipette tip module 77.

[0040] Additionally, refer to Figure 1 and Figure 3As shown, the reciprocating pipetting mechanism 7 includes a lifting plate 71, a first motor 72, a driving toothed pulley 73, a rotating shaft 74, a driven toothed pulley 75, a toothed belt 76, a pipetting tip module 77, and a drive unit 78. The first motor 72 is mounted on one bottom end of the lifting plate 71. The first motor 72 is a forward and reverse rotating motor. The output end of the first motor 72 is fixedly connected to the driving toothed pulley 73. The other bottom end of the lifting plate 71 is rotatably connected to the rotating shaft 74. The bottom end of the rotating shaft 74 is fixedly connected to the driven toothed pulley 75. A toothed belt 76 is connected between the driving toothed pulley 75, the driving toothed pulley 73, and the driven toothed pulley 75. Two sets of pipette tip modules 77 are fixed at the bottom of the toothed belt 76. The two sets of pipette tip modules 77 are respectively fixed on the toothed belt segments with opposite transmission directions. A drive component 78 is provided between the top center of the lifting plate 71 and the housing 1. The controller 3 is electrically connected to the drive component 78 and the first motor 72. Stabilizing components 79 are provided between the top two sides of the lifting plate 71 and the housing 1.

[0041] It should be noted that, in this embodiment, the driving component 78 is either an electric telescopic rod or a hydraulic push rod.

[0042] It should be noted that, in this embodiment, the number of pipette tips on the pipette tip module 77 is the same as the number of micro-holes in a horizontal row on the microwell plate 6, and their positions correspond one-to-one. That is, the distance between two adjacent pipette tips is the same as the distance between two adjacent horizontal micro-holes, and the distance between the two sets of pipette tip modules 77 is the same as the distance between two vertical micro-holes on the microwell plate 6.

[0043] During microplate pipetting, the microplate 6 is fixed within the microplate limiting frame 5, and the culture medium container 10 is fixed within the culture medium container limiting frame 9. The first motor 72 drives the active toothed belt pulley 73, which, in conjunction with the driven toothed belt pulley 75, drives the toothed belt 76 to rotate. The first motor 72 rotates forward, causing the toothed belt 76 to rotate forward, moving the two sets of pipette tip modules 77 in a direction away from each other. This moves the two sets of pipette tip modules 77 above the two culture medium containers 10. Then, the drive unit 78 drives the lifting plate 71 and the pipette tip modules 77 to descend, placing the pipette tip modules 77 into the culture medium container 10 to aspirate the cell culture medium. After the pipette tip modules 77 have aspirated the cell culture medium, the drive unit 78 resets. Then, the first motor 72 reverses, driving the toothed belt 76 to reverse, causing the two sets of pipette tips to rotate. The pipette tip modules 77 move towards each other. When the two sets of pipette tip modules 77 reach above the microplate 6, their positions overlap when viewed from the front. At this time, the pipette tip modules 77 are respectively placed above two adjacent sets of horizontally arranged microwells on the microplate 6. Then, the driving component 78 drives the lifting plate 71 and the pipette tip modules 77 to descend, placing the pipette tips on the pipette tip modules 77 into the microwells to add cell culture medium. After the addition is completed, the driving component 78 drives the lifting plate 71 and the pipette tip modules 77 to reset again, repeating the above operation to aspirate cell culture medium. During this process, the driving mechanism drives the moving plate 4 to move the microplate 6 backward by one station (one station is the distance between two longitudinal microwells), thereby realizing the simultaneous addition of two different cell culture media to the microplate 6, greatly improving the efficiency of pipetting and adding samples.

[0044] It should be noted that during the process of the reciprocating pipetting mechanism 7 pipetting and adding samples to the microplate 6, when liquid is added to the microwells in the horizontal row at the front and rear ends of the microplate 6, one set of pipetting tip modules 77 is placed outside the microplate 6. At this time, this set of pipetting tip modules 77 is in a non-dispensing state.

[0045] Reference Figure 1 and Figure 2 The drive mechanism includes a second motor 14, a lead screw 15, a bearing seat 16, and a threaded sleeve 11. The second motor 14 is installed on the rear side of the bottom of the housing 1. The second motor 14 is a forward and reverse motor. The second motor 14 is electrically connected to the controller 3. The output end of the second motor 14 is fixedly connected to the lead screw 15. The lead screw 15 is arranged longitudinally. Both ends of the lead screw 15 are fixed to the housing 1 through the bearing seat 16. The threaded sleeve 11 is threaded onto the lead screw 15 and is installed at the bottom of the moving plate 4.

[0046] During the process of pipetting and adding liquid to the microplate 6 by the reciprocating pipetting mechanism 7, after the two sets of pipetting head modules 77 have added liquid to the microplate 6, the second motor 14 drives the lead screw 15 to rotate, which drives the threaded sleeve 11 and the moving plate 4 to move backward, and then drives the microplate 6 to move backward one station, so as to cooperate with the reciprocating pipetting mechanism 7 to perform pipetting and adding liquid to all the microwells on the microplate 6.

[0047] Furthermore, first slide rails 12 are symmetrically arranged on both sides of the lead screw 15. The first slide rails 12 are parallel to the lead screw 15 and are installed at the bottom of the housing 1. A first slider 13 is slidably connected to the first slide rail 12 and is installed at the bottom of the moving plate 4. The moving plate 4 and the housing 1 are slidably connected by the first slide rail 12 and the first slider 13. The first slide rail 12 and the first slider 13 guide and stabilize the moving plate 4, ensuring smoother movement of the moving plate 4.

[0048] During the up-and-down movement of the lifting plate 71, in order to improve the stability of the movement of the lifting plate 71, refer to Figure 4 The stabilizing component 79 includes a fixed plate 791 and a telescopic plate 792. The fixed plate 791 has an internal cavity, and limit grooves 793 are vertically provided on both sides of the cavity. The telescopic plate 792 is placed inside the cavity of the fixed plate 791, and limit blocks 794 that slide with the limit grooves 793 are provided on both sides of its outer wall. The top of the fixed plate 791 is fixed to the top of the inside of the housing 1, and the bottom of the telescopic plate 792 is fixed to the top of the lifting plate 71. With the telescopic plate 792 and the fixed plate 791, the telescopic length can be adjusted in conjunction with the driving component 78, and the support for the lifting plate 71 can be improved, greatly enhancing the stability of the movement of the lifting plate 71.

[0049] To facilitate the removal and replacement of microplate 6, refer to... Figure 5 The microporous plate limiting frame 5 has through slots 51 on both sides, and the movable plate 4 has grooves 41 corresponding to the through slots 51. When removing the microporous plate 6, the operator inserts his finger into the groove 41, and the finger passes through the through slot 51 to lift the microporous plate 6 upward, which facilitates the removal and replacement of the microporous plate 6.

[0050] To facilitate the removal of culture medium box 10, refer to... Figure 1 and Figure 2 The container plate 8 has two longitudinally arranged second slide rails 17 on both sides below it. The second slide rails 17 are installed at the bottom of the box body 1. A second slider 18 is slidably connected to the second slide rails 17 and is installed at the bottom of the container plate 8. The container plate 8 and the box body 1 are slidably connected by the second slide rails 17 and the second slider 18, which facilitates moving the container plate 8 to the opening of the box body 1 to replace or remove the culture medium box 10.

[0051] When the microplate pipetting device is not in operation, for easy storage of the pipette tip module 77, refer to... Figure 5 and Figure 6 The receiving plate 8 has a receiving cavity 81 inside. The top of the receiving plate 8 is located within the culture medium box limiting frame 9 and has several positioning members 82. The number of positioning members 82 is the same as the number of pipette tips on the pipette tip module 77 and their positions are one-to-one. That is, the distance between two adjacent pipette tips is equal to the distance between two adjacent positioning members 82. The positioning members 82 pass through the top of the receiving plate 8 and are connected to the receiving cavity 81. In this embodiment, the positioning member 82 is a through conical cylinder and the width of the top end of the positioning member 82 is greater than the width of the bottom end. The receiving cavity 81 has a collection box 83 and a handle 84 is provided on the outer wall of the collection box 83. When the microplate pipetting device is not in operation, the culture medium box 10 on the receiving plate 8 is removed, and then the two sets of pipette tip modules 77 are moved above the receiving plate 8 so that the pipette tips are vertically aligned with the positioning member 82. The lifting plate 71 and the pipette tip module 77 are driven to descend by the driving member 78 to store the pipette tips in the positioning member 82, thus avoiding contamination of the pipette tips. The culture medium remaining on the pipette tips flows into the collection box 83 for collection, which will not cause contamination to the inside of the box 1 and avoid the possibility of cross-contamination.

[0052] Reference Figure 2 A positioning plate 19 is provided on the rear side of the receiving plate 8. The positioning plate 19 is fixed to the bottom of the inside of the box 1, and the receiving plate 8 and the positioning plate 19 are in close contact. The positioning plate 19 ensures that the receiving plate 8 moves into place, ensuring that the pipette tip module 77 can enter the culture medium box 10 to pick up liquid, and also ensuring that the pipette tip can be stored in the positioning component 82.

[0053] In this embodiment, to improve the tight fit between the receiving plate 8 and the positioning plate 19, an electromagnet is embedded at one end of the positioning plate 19 near the receiving plate 8. The electromagnet is electrically connected to the controller 3, and its outer surface is flush with the outer surface of the positioning plate 19. An iron sheet is embedded on one side of the receiving plate 8 near the positioning plate 19, and its outer surface is flush with the outer surface of the receiving plate 8. This allows the iron sheet to be attracted when the receiving plate 8 is positioned and fixed, thereby fixing the position of the receiving plate 8 and ensuring consistency in position each time. When the receiving plate 8 is moved, the electromagnet is de-energized, thus disengaging the fixing of the receiving plate 8 and facilitating its movement.

[0054] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A microplate pipetting device, characterized in that: The device includes a housing (1), a microplate (6) located at the bottom of the housing (1), culture medium boxes (10) located on both sides of the microplate (6), and a reciprocating pipetting mechanism (7) located on the top of the housing (1). The reciprocating pipetting mechanism (7) is used to pipe culture medium from the culture medium boxes (10) on both sides into the micropores on the microplate (6). The housing (1) has a sealing door (2) at its opening end, and a controller (3) is located on one side of the outer wall of the housing (1). The microporous plate (6) is provided with a movable plate (4) at the bottom and a microporous plate limiting frame (5) at the top of the movable plate (4). The microporous plate (6) is placed inside the microporous plate limiting frame (5). The box (1) is provided with a driving mechanism that drives the movable plate (4) to move longitudinally. The bottom of the culture medium box (10) is provided with a receiving plate (8), and the top of the receiving plate (8) is provided with a culture medium box limiting frame (9). The culture medium box (10) is placed inside the culture medium box limiting frame (9). The reciprocating pipetting mechanism (7) includes a lifting plate (71), a first motor (72), a driving toothed pulley (73), a rotating shaft (74), a driven toothed pulley (75), a toothed belt (76), a pipetting tip module (77), and a drive component (78). The first motor (72) is mounted on one end of the bottom of the lifting plate (71), and the output end of the first motor (72) is fixedly connected to the driving toothed pulley (73). The rotating shaft (74) is rotatably connected to the other end of the bottom of the lifting plate (71), and the bottom end of the rotating shaft (74) is fixedly connected to the drive component (73). A driven toothed pulley (75) is fixedly connected to the driven toothed pulley (73), and a toothed belt (76) is connected between the driven toothed pulley (75). Two sets of pipette tip modules (77) are fixed at the bottom end of the toothed belt (76). The two sets of pipette tip modules (77) are respectively fixed on the toothed belt segments with opposite transmission directions of the toothed belt (76). A driving component (78) is provided between the top center of the lifting plate (71) and the housing (1). Stabilizing components (79) are provided between the top two sides of the lifting plate (71) and the housing (1).

2. The microplate pipetting device according to claim 1, characterized in that: The drive mechanism includes a second motor (14), a lead screw (15), a bearing seat (16), and a threaded sleeve (11). The second motor (14) is installed on the rear side of the bottom of the housing (1). The output end of the second motor (14) is fixedly connected to the lead screw (15). The lead screw (15) is arranged longitudinally. Both ends of the lead screw (15) are fixed to the housing (1) through the bearing seat (16). The threaded sleeve (11) is threaded onto the lead screw (15) and is installed at the bottom of the moving plate (4).

3. The microplate pipetting device according to claim 2, characterized in that: The lead screw (15) is symmetrically provided with first slide rails (12) on both sides. The first slide rails (12) are installed at the bottom of the box (1). A first slider (13) is slidably connected on the first slide rails (12). The first slider (13) is installed at the bottom of the moving plate (4).

4. The microplate pipetting device according to claim 1, characterized in that: The stabilizing component (79) includes a fixed plate (791) and a telescopic plate (792). The fixed plate (791) has a cavity inside, and limit grooves (793) are vertically provided on both sides of the cavity. The telescopic plate (792) is placed inside the cavity of the fixed plate (791), and limit blocks (794) that are slidably connected to the limit grooves (793) are provided on both sides of its outer wall. The top of the fixed plate (791) is fixed to the top of the box (1), and the bottom of the telescopic plate (792) is fixed to the top of the lifting plate (71).

5. A microplate pipetting device according to claim 1, characterized in that: The microporous plate limiting frame (5) has through slots (51) on both sides, and the movable plate (4) has grooves (41) corresponding to the through slots (51).

6. The microplate pipetting device according to claim 1, characterized in that: The receiving plate (8) is provided with a second slide rail (17) on both sides below it. The second slide rail (17) is installed at the bottom of the box (1). A second slider (18) is slidably connected on the second slide rail (17). The second slider (18) is installed at the bottom of the receiving plate (8).

7. A microplate pipetting device according to claim 6, characterized in that: The receiving plate (8) has a receiving cavity (81) inside. The top of the receiving plate (8) is located inside the culture medium box limiting frame (9) and has several positioning parts (82). The positioning parts (82) pass through the top of the receiving plate (8) and communicate with the receiving cavity (81). The receiving cavity (81) has a liquid collection box (83) inside. The outer wall of the liquid collection box (83) has a handle (84).

8. A microplate pipetting device according to claim 6, characterized in that: A positioning plate (19) is provided on the rear side of the receiving plate (8). The positioning plate (19) is fixed to the bottom of the box (1) and the receiving plate (8) and the positioning plate (19) are closely fitted together.

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