Nucleic acid detection device

Abstract

The present application provides a nucleic acid detection device, comprising a detection device main body and a reagent tube assembly, the reagent tube assembly comprises a sample tube for containing a sample and a reagent and a reaction hole capable of controllable communication with the sample tube, the detection device main body is provided with a heating assembly, the heating assembly has a heating groove, and the reagent tube assembly can be placed on the heating assembly so that the reaction hole is partially in the heating groove. The reaction hole of the present application is a component of the reagent tube assembly, which is no longer in the reaction device of the detection device, does not pollute the detection device main body, and the detection device can be reused. During use, the user only needs to replace the reagent tube assembly, which greatly reduces the detection and use cost of the user.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of nucleic acid detection device design, and particularly relates to a nucleic acid detection device. BACKGROUND

[0002] With the improvement of people's living standards and the development of biotechnology, people's pursuit and concern for health are increasingly prominent. At the same time, with the acceleration of people's life rhythm, time, cost, privacy and the like have become aspects of concern in work and life. In particular, in medical detection, cumbersome examinations in hospitals, long waiting time for results, high detection costs, and even some private detection projects have plagued people. In order to overcome the foregoing deficiencies, a portable nucleic acid detection device appears in the prior art, which is small in size, compact in structure, and can be purchased by users for self-detection at home. After the corresponding reagent tube is added with sample and completed, the sample flows into each reaction well in turn through the flow channel, and the result is read after the reaction is completed. Since each reaction well is in the reaction device of the detection device, the reaction device is contaminated after completing the nucleic acid detection of the user at this time, so that the detection device cannot be reused, which increases the detection use cost of the user. SUMMARY

[0003] Therefore, the technical problem to be solved by the present application is to provide a nucleic acid detection device to overcome the deficiency that the reaction well in the prior art is in the reaction device of the detection device, which is contaminated after completing the nucleic acid detection once, so that the detection device cannot be reused, thereby increasing the detection use cost of the user.

[0004] In order to solve the foregoing problem, the present application provides a nucleic acid detection device, which comprises a detection device main body and a reagent tube assembly. The reagent tube assembly comprises a sample tube for containing a sample and a reagent, and a reaction well capable of controllable communication with the sample tube. The detection device main body is provided with a heating assembly, and the heating assembly is provided with a heating groove. The reagent tube assembly can be placed on the heating assembly so that the reaction well is partially in the heating groove.

[0005] In some embodiments, the heating assembly comprises a heating mounting seat, and the heating groove is configured on the heating mounting seat.

[0006] In some embodiments, the reaction well has a plurality of reaction wells, the number of the heating grooves is the same as and one-to-one corresponds to the number of the reaction wells, and each of the heating grooves is provided with a first input optical fiber mounting hole and a first output optical fiber mounting hole.

[0007] In some embodiments, the first end of the sample tube has a sealing cover, and the second end is a closed end, the reagent tube assembly further comprises a base having a positioning hole for inserting the second end of the sample tube, and a piercing head with a hollow flow channel is arranged in the positioning hole, when the sample tube is inserted into the positioning hole, the piercing head can pierce the closed end to make the content contained in the sample tube flow into the reaction hole through the hollow flow channel.

[0008] In some embodiments, the base comprises a fixing disc and a reaction seat, the fixing disc and the reaction seat are connected with each other by interlocking, the positioning hole is arranged on the fixing disc, and the base further comprises a distribution disc, the piercing head is connected to the distribution disc, the distribution disc has a distribution flow channel, one end of the distribution flow channel communicates with the hollow flow channel, and the other end of the distribution flow channel communicates with the reaction hole.

[0009] In some embodiments, the distribution flow channel is an open slot arranged on the bottom end surface of the distribution disc, and the base further comprises a sealing film, the sealing film is clamped between the reaction seat and the bottom end surface of the distribution disc to seal the opening of the open slot.

[0010] In some embodiments, the sealing film is a silica gel film, the distribution flow channel has a plurality of distribution flow channels, the first ends of the plurality of distribution flow channels communicate with the hollow flow channel, and the second ends of the plurality of distribution flow channels each communicate with one reaction hole, and the first ends of the distribution flow channels can controllably block or pass the silica gel film.

[0011] In some embodiments, a first hemispherical groove is arranged at a central position of the distribution disc, the first ends of the plurality of distribution flow channels are on the groove wall of the first hemispherical groove, the silica gel film has a second hemispherical groove, the second hemispherical groove is arranged opposite to the first hemispherical groove, and the reaction seat has a through hole at a position corresponding to the second hemispherical groove.

[0012] In some embodiments, the nucleic acid detection device further comprises a mounting top plate, the heating assembly is fixedly connected to the top surface of the mounting top plate, the mounting top plate is provided with a top rod, and the top rod can be controlled to pass through the through hole to apply force to or release force from the bottom surface of the second hemispherical groove.

[0013] In some embodiments, the nucleic acid detection device further comprises a motion scanning assembly, the motion scanning assembly comprises an optical assembly and a driving assembly, and the driving assembly comprises a synchronous wheel sleeved on the top rod to be able to rotate around the top rod.

[0014] The present invention provides a nucleic acid detection device in which the reaction well is an integral part of the reagent tube assembly and is no longer located within the reaction device of the detection device. This prevents contamination of the main body of the detection device and allows the detection device to be reused. During use, the user only needs to replace the reagent tube assembly, which greatly reduces the user's detection cost. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the nucleic acid detection device according to an embodiment of the present invention (excluding components such as the outer shell);

[0016] Figure 2 This is a three-dimensional structural diagram of the nucleic acid detection device according to another perspective of an embodiment of the present invention (excluding components such as the outer shell);

[0017] Figure 3 for Figure 1 A schematic diagram of the heating component in the diagram;

[0018] Figure 4 for Figure 1 A schematic diagram showing the assembly positions of the reagent tube assembly, heating assembly, and push rod.

[0019] Figure 5 for Figure 1 A schematic diagram of the internal structure of the reagent tube assembly;

[0020] Figure 6 for Figure 5 A schematic diagram of the sample tube structure in the image;

[0021] Figure 7 for Figure 5 A three-dimensional structural diagram of the fixed disk in the image;

[0022] Figure 8 for Figure 5 A three-dimensional structural diagram of the reaction seat in the middle;

[0023] Figure 9 for Figure 5 A schematic diagram of the internal structure of the distribution disk.

[0024] The reference numerals in the attached figures are as follows:

[0025] 10, reagent tube assembly; 1, sample tube; 11, sealing cover; 12, first clamping groove; 13, insertion hole; 21, piercing head; 22, fixing disc; 221, first elastic buckle; 222, second elastic buckle; 223, air passage hole; 23, reaction seat; 231, reaction hole; 232, through hole; 233, second clamping groove; 234, positioning pin; 235, foolproof structure; 24, liquid separation disc; 241, flow guide column; 242, first half-spherical groove; 243, insertion column; 244, air hole; 25, sealing film; 251, second half-spherical groove; 20, heating assembly; 202, heating mounting seat; 203, first input optical fiber mounting hole; 204, first output optical fiber mounting hole; 205, heating control board; 206, heating element; 207, heating sheet; 301, mounting top plate; 302, top rod; 303, bottom plate; 304, support rod; 305, second input optical fiber mounting hole; 401, optical assembly; 4021, driving motor; 4022, synchronous wheel; 4023, synchronous belt; 4024, linear guide rail; 501, signal acquisition module. DETAILED DESCRIPTION

[0026] BRIEF DESCRIPTION OF DRAWINGS Figures 1 to 9 According to the embodiment of the present application, please refer to Figure 1 and Figure 2 According to the embodiment of the present application, please refer to and

[0027] In some embodiments, the heating assembly 20 comprises a heating mounting base 202, which is sequentially stacked with a heating element 206 and a heating control board 205, wherein the heating control board 205 is used to control the operation of the heating element 206, and the heating element 206 is specifically, for example, a PI heating film or a ceramic heating sheet, a Peltier element or other heating elements. The heating groove is configured on the heating mounting base 202, so as to realize efficient heating and heat preservation of the reaction hole 231. In an embodiment, the heating mounting base 202 is further provided with a heating sheet 207 between the heating element 206 and the heating mounting base 202, which can cover the heating element 206 below and play a role of aesthetics and protection of the heating element 206.

[0028] In some embodiments, the reaction hole 231 has a plurality of reaction holes, and the number of heating grooves is the same as and one-to-one corresponds to the number of reaction holes 231, so as to realize synchronous detection of multiple indexes and improve the detection efficiency. The first input optical fiber mounting hole 203 and the first output optical fiber mounting hole 204 are arranged at each heating groove. Specifically, the first input optical fiber mounting hole 203 and the first output optical fiber mounting hole 204 are respectively connected with a transmission optical fiber, so that the excitation light and the fluorescence can be flexibly transmitted to the corresponding components, for example, the excitation light is transmitted from the optical assembly 401 to the reaction hole 231 through the input optical fiber, so as to excite the sample solution in the reaction hole 231, and the fluorescence signal excited in the reaction hole 231 is transmitted to the signal acquisition module 501 through the output optical fiber, so as to obtain the final detection result. Referring to Figure 1 As shown, the second input optical fiber mounting hole 305 is configured on the mounting top plate 301, which is connected with the input optical fiber between the first input optical fiber mounting hole 203. Similarly, the output optical fiber is connected between the first output optical fiber mounting hole 204 and the second output optical fiber mounting hole (not shown in the figure) of the signal acquisition module 501. The signal acquisition module 501 can be MPPC acquisition, or PD acquisition, or photographing acquisition, or color recognition. The signal acquisition module 501 and the optical assembly 401 can be combined into one body, or can be separated and use optical fiber light guide. Figure 1 The signal acquisition module 501 and the optical assembly 401 can be combined into one body, or can be separated and use optical fiber light guide.

[0029] In some embodiments, the first end of the sample tube 1 has a sealing cover 11, and the second end is a closed end. The reagent tube assembly 10 further comprises a base having a positioning hole into which the second end of the sample tube 1 is inserted, and a piercing head 21 with a hollow flow channel is arranged in the positioning hole. When the sample tube 1 is inserted into the positioning hole, the piercing head 21 can pierce the closed end to allow the content (i.e. the mixed liquid of the sample and the reagent) in the sample tube 1 to flow into the reaction hole 231 through the hollow flow channel. At this time, the reagent tube assembly specifically forms a plug-in type reagent tube assembly. In this technical solution, the sample tube 1 can be connected to the base in a plug-in manner. During the plug-in process of the two, the piercing head 21 can pierce the closed end of the sample tube 1 to allow the content in the sample tube 1 to flow into the reaction hole 231 with a certain volume. In specific applications, only the user needs to sample and then place the sample into the sample tube 1, and then insert and connect the sample tube 1 with the base. The quantitative transfer of the sample into the reaction hole 231 can be realized. Finally, the reagent tube assembly is placed on the detection device main body to perform subsequent heating amplification, optical detection and other steps without the need for special pipetting and quantitative processes. The operation is very simple and convenient, especially suitable for home self-testing scenarios, thereby meeting the user's privacy protection purpose and family sharing, and saving a lot of waiting time. It should be noted that the sealing cover 11 is provided with a gas passage hole, and a breathable film is covered on the gas passage hole by ultrasonic or hot melting method to ensure that the content in the sample tube 1 can smoothly enter the reaction hole 231 through the piercing head 21 in a sealed state (i.e. the sealing cover 11 is in a sealed state).

[0030] Referring to Figure 5 As shown, the base comprises a fixed disc 22 and a reaction seat 23. The fixed disc 22 and the reaction seat 23 are connected to each other by mutual buckling, that is, they are assembled in a stack to realize the assembly and corresponding operation of the components (such as a distribution disc 24) arranged inside. The positioning hole is arranged on the fixed disc 22, and the base further comprises the distribution disc 24. The piercing head 21 is connected to the distribution disc 24. The distribution disc 24 has a distribution flow channel. One end of the distribution flow channel is in communication with the hollow flow channel, and the other end of the distribution flow channel is in communication with the reaction hole 231, thereby realizing smooth transfer and guidance of the content in the sample tube 1 into the reaction hole 231. Further, the distribution disc 24 further has a flow guide column 241 extending into the reaction hole 231. The distribution flow channel communicates with the reaction hole 231 through the flow guide column 241. In this technical solution, the flow guide column 241 extends into the reaction hole 231, preferably into the bottom of the reaction hole 231, which can prevent the sample solution droplets from forming a pressure difference between the inlet and the bottom of the reaction hole 231, so that the liquid cannot fill the entire reaction hole 231 and cannot achieve the purpose of quantification.

[0031] In a specific embodiment, referring to Figure 9As shown, the liquid distribution flow channel is an open groove formed on the bottom end surface of the liquid distribution disc 24, and the base further comprises a sealing film 25 clamped between the reaction base 23 and the bottom end surface of the liquid distribution disc 24 to seal the opening of the open groove. The open groove is used to form the liquid distribution flow channel, which can reduce the processing difficulty of the liquid distribution flow channel, and the sealing film 25 can ensure the sealing of the liquid distribution flow channel.

[0032] In some embodiments, the sealing film 25 is a silica gel film, so that the sealing film 25 has elastic deformation capability. The liquid distribution flow channel has a plurality of first ends in communication with the hollow flow channel and a plurality of second ends each in communication with a reaction hole 231. The first end of the liquid distribution flow channel can be controllably blocked or conducted by the silica gel film, that is, the blocking and conducting of the liquid distribution flow channel can be realized by controlling the deformation of the silica gel film, so as to maintain the internal sealed environment during the amplification or detection of the sample liquid in the reaction hole 231 and prevent pollution to the external environment. In a specific embodiment, a first hemispherical groove 242 is formed at the central position of the liquid distribution disc 24, the opening of the first hemispherical groove 242 faces downward, the first end of the plurality of liquid distribution flow channels is located on the groove wall of the first hemispherical groove 242, the silica gel film has a second hemispherical groove 251 with an upward opening, the second hemispherical groove 251 is oppositely arranged with the first hemispherical groove 242, and the reaction base 23 is provided with a through hole 232 corresponding to the second hemispherical groove 251. In this technical solution, a substantially spherical accommodation space is formed between the first hemispherical groove 242 and the second hemispherical groove 251, and a freeze-dried reagent can be preloaded in the accommodation space. When nucleic acid amplification or other subsequent operations are required, a top rod or other ejection component on the main body of the corresponding detection device can be used to apply force to the bottom surface of the second hemispherical groove 251 to make it deform upward and finally fit with the groove wall of the first hemispherical groove 242, so as to block the liquid distribution flow channel. It can be understood that when the liquid distribution flow channel does not need to be blocked, that is, needs to be conducted, the ejection component should be removed from the second hemispherical groove 251, and under the action of the elastic force of the silica gel film, it will be concave downward, thereby realizing the conducting of the liquid distribution flow channel. Specifically, the nucleic acid detection device further comprises a mounting top plate 301, and the heating assembly 20 is fixedly connected to the top surface of the mounting top plate 301. The mounting top plate 301 is provided with a top rod 302 which can be controlled to pass through the through hole 232 to apply force to or remove the force from the bottom surface of the second hemispherical groove 251. Specifically, the top rod 302 can be lifted or lowered, and when it is lifted, the top thereof applies force to the bottom surface of the second hemispherical groove 251, and when it is lowered, the force is removed.

[0033] In some embodiments, the nucleic acid detection device further comprises a motion scanning assembly for generating corresponding excitation light. Specifically, referring to Figure 2As shown, the motion scanning assembly includes an optical assembly 401 and a driving assembly, the driving assembly includes a driving motor 4021 and a synchronous wheel 4022 sleeved on the top rod 302 to be able to rotate around the top rod 302, a synchronous belt 4023 is in tension connection between the output shaft of the driving motor 4021 and the synchronous wheel 4022, the optical assembly 401 is fixedly connected with the synchronous belt 4023 to realize the purpose that the rotation of the synchronous belt 4023 drives the position switching of the optical assembly 401, and in order to ensure the smoothness and reliability of the position switching of the optical assembly 401, a linear guide rail 4024 is further arranged between the mounting top plate 301 and the optical assembly 401. It needs to be particularly pointed out that in this technical solution, the top rod 302 serves as a control component for the conduction of the liquid distribution channel on one hand, and serves as a mounting and positioning component of the synchronous wheel 4022 and a rotating shaft on the other hand, which simplifies the structure of the nucleic acid detection device and is conducive to the compactness and miniaturization design of the device, thereby improving the portability of the device.

[0034] The number of each optical fiber mounting hole can be designed according to the number of detection items, in a specific embodiment, see Figure 1 As shown, the second input optical fiber mounting hole 305 has six, the corresponding first input optical fiber mounting hole 203, the second output optical fiber mounting hole 204 and the second input optical fiber mounting hole all have six, and the corresponding reaction hole 231 also has six, and the synchronous output excitation light of the optical assembly 401 is only provided with two groups, and the position of the optical assembly 401 is moved, for example, front, middle and rear, to realize the transmission of six groups of excitation light and the detection of the contents in the six reaction holes 231.

[0035] Specifically, see Figure 7 As shown, the top surface of the fixed disc 22 is provided with a first elastic buckle 221, and at least two first elastic buckles 221 are arranged at intervals around the positioning hole, and the outer circumferential wall of the second end of the sample tube 1 has a first clamping groove 12 matched with the first elastic buckle 221, and the first clamping groove 12 can be, for example, a ring groove arranged around the tube body of the sample tube 1, or it can be a single plurality of grooves, and the present application does not make special limitation, and the quick, convenient and reliable connection between the sample tube 1 and the base is realized through the clamping connection relationship between the first elastic buckle 221 and the first clamping groove 12.

[0036] The bottom surface of the fixed disc 22 is provided with a second elastic buckle 222, and at least two second elastic buckles 222 are arranged at intervals around the fixed disc 22, and the outer circumferential wall of the reaction seat 23 has a second clamping groove 233 matched with the second elastic buckle 222, so as to realize the quick and reliable assembly between the reaction seat 23 and the fixed disc 22.

[0037] See Figure 9As shown, the distribution disc 24 is configured with air holes 244, the number of air holes 244 is the same as the number of reaction holes 231 and one-to-one correspondence, the fixed disc 22 is configured with air holes 223, the air holes 223 are provided with air plugs (not shown in the figure), the air plugs are air-permeable and water-impermeable structure, so as to realize the smooth discharge of the gas in the reaction hole 231, and further ensure that the contents in the sample tube 1 smoothly enter the reaction hole 231.

[0038] The fixed disc 22 and the reaction seat 23 have positioning pins 234, as shown in Figure 8 The positioning pins 234 have two, the two positioning pins 234 are integrally formed with the reaction seat 23, at this time the bottom surface of the corresponding distribution disc 24 is configured with corresponding positioning holes, the positioning pins 234 and the positioning holes are inserted and matched, the position of the distribution disc 24 and the reaction hole 231 on the reaction seat 23 is determined.

[0039] In some embodiments, the reaction seat 23 also has a foolproof structure 235, for example, a protrusion protruding from the outer circumferential wall of the reaction seat 23, which can cooperate with the corresponding parts such as the heating module on the detection device body to achieve the positioning purpose.

[0040] As shown in Figure 9 The fixed disc 22 has an upwardly protruding plug-in column 243, the plug-in column 243 is fixedly connected with the puncture head 21, the second end of the sample tube 1 has a plug-in hole 13 matched with the plug-in column 243, through which the puncture head 21 can be precisely and quickly positioned, and the connection of the sample tube 1 can be more reliable. As shown in Figure 5 The reaction hole 231 protrudes downward from the seat body plane of the reaction seat 23, so as to be placed in the corresponding heating groove of the heating module to realize multi-face wrapping and ensure the temperature control and heat preservation effect.

[0041] It should be noted that the various components involved in the reagent tube assembly 10 of the present application are formed by injection molding, that is, formed by injection molding, which has low manufacturing cost and can be discarded after being used as conventional consumables.

[0042] It is easy for those skilled in the art to understand that the above advantageous modes can be freely combined and superimposed without conflict.

[0043] The above is only the preferred embodiment of the present application, and does not limit the present application, any modification, equivalent replacement and improvement within the spirit and principle of the present application shall be included in the protection scope of the present application. The above is only the preferred embodiment of the present application, it should be pointed out that for ordinary skilled in the art, without departing from the technical principles of the present application, a number of improvements and modifications can be made, which should be regarded as the protection scope of the present application.

Claims

1. A nucleic acid detection device, characterized by, The detection device comprises a detection device body and a reagent tube assembly (10), the reagent tube assembly (10) comprises a sample tube (1) for containing samples and reagents and a reaction hole (231) capable of controllable communication with the sample tube (1), the detection device body is provided with a heating assembly (20) having a heating groove, and the reagent tube assembly (10) can be placed on the heating assembly (20) so that the reaction hole (231) is partially in the heating groove. The first end of the sample tube (1) is provided with a sealing cover (11), and the second end is a closed end. The reagent tube assembly (10) further comprises a base provided with a positioning hole for inserting the second end of the sample tube (1), and a puncture head (21) with a hollow flow channel is arranged in the positioning hole. When the sample tube (1) is inserted into the positioning hole, the puncture head (21) can pierce the closed end so that the contents contained in the sample tube (1) flow into the reaction hole (231) through the hollow flow channel. The base comprises a fixing disc (22) and a reaction seat (23), the fixing disc (22) and the reaction seat (23) are connected by mutual buckling, the positioning hole is arranged on the fixing disc (22), and a distribution disc (24) is further arranged, the puncture head (21) is connected to the distribution disc (24), the distribution disc (24) is provided with a distribution flow channel, one end of the distribution flow channel communicates with the hollow flow channel, and the other end of the distribution flow channel communicates with the reaction hole (231). The distribution flow channel is an open groove arranged on the bottom end face of the distribution disc (24), and the base further comprises a sealing film (25), the sealing film (25) is clamped between the reaction seat (23) and the bottom end face of the distribution disc (24) to seal the opening of the open groove.

2. The nucleic acid detection device of claim 1, wherein, The heating assembly (20) comprises a heating mounting seat (202), the heating groove is arranged on the heating mounting seat (202), and the reaction hole (231) has a plurality of.

3. The nucleic acid detection device of claim 2, wherein, The number of the heating grooves is the same as and corresponds to the number of the reaction holes (231), and a first input optical fiber mounting hole (203) and a first output optical fiber mounting hole (204) are arranged at each of the heating grooves.

4. The nucleic acid detection device of claim 1, wherein The sealing film (25) is a silica gel film, the distribution flow channel has a plurality of, the first ends of the plurality of distribution flow channels communicate with the hollow flow channel, and the second ends of the plurality of distribution flow channels each communicate with one of the reaction holes (231), and the first ends of the distribution flow channels can be controllably blocked or conducted by the silica gel film.

5. The nucleic acid detection device of claim 4, wherein, A first hemispherical groove (242) is arranged at the central position of the distribution disc (24), the first ends of the plurality of distribution flow channels are located on the groove wall of the first hemispherical groove (242), the silica gel film is provided with a second hemispherical groove (251), the second hemispherical groove (251) is arranged opposite to the first hemispherical groove (242), and the reaction seat (23) is provided with a through hole (232) at a position corresponding to the second hemispherical groove (251).

6. The nucleic acid detection device of claim 5, wherein, Further comprising a mounting top plate (301), the heating assembly (20) is fixedly connected to the top surface of the mounting top plate (301), the mounting top plate (301) is provided with a top rod (302), the top rod (302) can be controlled to pass through the through hole (232) to apply force to or release the force applied to the bottom surface of the second half-spherical groove (251).

7. The nucleic acid detection device of claim 6, wherein, Further comprising a motion scanning assembly, the motion scanning assembly comprises an optical assembly (401) and a driving assembly, the driving assembly comprises a synchronous wheel (4022) sleeved on the top rod (302) to enable rotation around the top rod (302).

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