A factory domestication method of litopenaeus vannamei "bohai No. 1" fry

Abstract

The application provides a factory domestication method of Litopenaeus vannamei 'Bohai No.1' fry, and is characterized in that the method comprises the following steps: pre-treating a culture pond, inoculating biological floc in the culture pond, feeding fish of the Perciformes into the culture pond to establish a biological floc culture system, temporarily culturing fry, and putting the fry into the culture pond. Through the pre-treatment of the culture pond, the establishment of the culture system and the domestication of the fry culture, the factory culture mode of the Litopenaeus vannamei can be realized, and a high Litopenaeus vannamei culture yield can be obtained. The average survival rate of the Litopenaeus vannamei 'Bohai No.1' fry cultured for 90 days is 85.8-93.3%, and the average weight of the individuals is 11.6-14.9g. The biological floc is successfully applied to the factory culture mode of the Litopenaeus vannamei, a high culture yield is achieved, and the diversification degree of the shrimp culture in China is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of prawn breeding, in particular to a factory domestication method of Litopenaeus vannamei 'Bohai No.1' seedlings. BACKGROUND

[0002] Litopenaeus vannamei, also known as White pacific shrimp, has a rapid growth in yield due to its many characteristics: (1) fast growth and short breeding cycle: it can be marketed in an average of 3 months; (2) strong adaptability: strong adaptability to environmental changes such as temperature, dissolved oxygen and salinity, especially wide salinity adaptation range, and can be bred in freshwater after gradual desalination; (3) high-density breeding tolerance: the highest yield per mu can reach 5000 kg; (4) diverse breeding modes: domestic breeding is carried out in Liaoning in the north, Hainan in the south, Xinjiang in the west, and southeast coastal areas in the east. In particular, the wide salinity adaptation range of the species has expanded its breeding range from seawater to inland freshwater, saline-alkali land and coastal high-salinity salt field areas. In recent years, with the upgrading and comprehensive development and utilization of the salt industry, the use of salted water areas for Litopenaeus vannamei breeding has developed rapidly.

[0003] According to statistics, the total salt field breeding area in China is 4 million mu, and the current utilization rate of salt field water surface is very low. The use of salt fields for Litopenaeus vannamei breeding has certain limitations for realizing the green industrial upgrading of enterprises and promoting the development of China's Litopenaeus vannamei breeding industry. For the factory breeding of Litopenaeus vannamei seedlings, the limitations of salt field water area breeding are reduced, and higher breeding yield is achieved, improving the diversification of China's prawn breeding. SUMMARY

[0004] Therefore, the present application aims to provide a factory domestication method of Litopenaeus vannamei 'Bohai No.1' seedlings, to realize the factory breeding mode of Litopenaeus vannamei and obtain higher breeding yield of Litopenaeus vannamei.

[0005] The technical scheme of the present application is as follows:

[0006] A factory domestication method of Litopenaeus vannamei 'Bohai No.1' seedlings, comprising the following steps:

[0007] S1: Pre-treatment of the breeding pond: disinfect the breeding pond and the water used for breeding, and lay the bottom mud at the bottom of the breeding pond;

[0008] S2 establishing a breeding system: inoculating biological floc in the breeding pond, the concentration of the biological floc is 42-48 mL / L, inoculating the biological floc for at least 2 days, and then putting the Perciformes fish into the breeding pond to establish a biological floc breeding system; the biological floc is obtained by cultivating purple non-sulfur bacteria and nitrosomonas, and the biological floc can provide natural bait for prawns, improve the utilization rate of bait, provide antibacterial activity for prawns, and purify water quality; the breeding process can be carried out without water change, effectively avoiding the introduction of exogenous pathogens, and reducing production cost;

[0009] S3 breeding and domestication of seedlings: temporarily breeding the "Bohai No. 1" seedlings, and putting them into the breeding pond at a density of 0.8-1 million / m 3 , the initial salinity of the water body is 30-33 salinity, the salinity of the water body is increased by 0.2-0.4 salinity per day, until the salinity of the water body is 62-65 salinity, and then the salinity of the water body is maintained at 62-65 salinity until the target prawns are harvested.

[0010] Further, in step S2, after inoculating the biological floc in the breeding pond, the pH value of the water body is adjusted to 8.6-9.0 and the C / N value is adjusted to 15-20 before the Perciformes fish is put into the breeding pond for breeding; after the Perciformes fish is put into the breeding pond for breeding, the pH value of the water body is adjusted to 7.4-7.6 and the C / N value is adjusted to 10-15; adjusting the pH value and C / N value of the water body helps to balance the dynamic competition process of algae, autotrophic bacteria and heterotrophic bacteria, and the Perciformes fish such as tilapia or sea bass is put into the breeding pond, and by feeding part of the biological floc combined with adjusting the C / N value, the biological floc recombines with inorganic matter, organic matter or suspended particles such as plankton in the water body of the breeding pond to continue to form new biological floc, continuously and stably establish a micro food web, and promote the nutrient cycle of the breeding water body.

[0011] Further, in step S2, the cultivation time for establishing the biological floc breeding system is at least 18 days.

[0012] Further, the density of the Perciformes fish is 100-150 tails / mu, and the weight is 200-300 g.

[0013] Further, the Perciformes fish is bred for at least 4 days, and further includes re-inoculating the biological floc, and the concentration of the biological floc is 20-25 mL / L.

[0014] Further, after re-inoculating the biological floc, the pH value of the water body is adjusted to 7.8-8.2 and the C / N value is adjusted to 8-10.

[0015] Further, the Perciformes fish is tilapia or sea bass.

[0016] Further, the preparation method of the bio-floc is: inoculating the complex bacterial inoculum and the complex algal inoculum in sterile water, adding glucose, mixing, aeration activation, adding clay, continuing aeration activation, and obtaining the bio-floc; the complex bacterial inoculum is purple non-sulfur bacteria and nitrosomonas, and the complex algal inoculum is chlorella marina and nannochloropsis; during the cultivation of the bio-floc, the complex algal inoculum and the clay are added, which can stimulate the formation of the floc and accelerate the occurrence of flocculation; the establishment of the stable bio-floc breeding system can realize the abilities of resisting escherichia coli and resisting environmental stress in the breeding pond mode.

[0017] Further, the preparation method of the bio-floc is: inoculating the complex bacterial inoculum and the complex algal inoculum in sterile water, adding glucose, mixing, aeration activation, adding clay, continuing aeration activation, and obtaining the bio-floc; the complex bacterial inoculum is purple non-sulfur bacteria and nitrosomonas, and the complex algal inoculum is chlorella marina and nannochloropsis; during the cultivation of the bio-floc, the complex algal inoculum and the clay are added, which can stimulate the formation of the floc and accelerate the occurrence of flocculation; the establishment of the stable bio-floc breeding system can realize the abilities of resisting escherichia coli and resisting environmental stress in the breeding pond mode.

[0018] Further, in step S3, after the fry is put, artificial compound bait is fed at least on the 3rd day, 3-5 times a day, and 2-3% of the weight of the shrimp fry each time.

[0019] Compared with the prior art, the beneficial effects of the present application are:

[0020] The present application can realize the factory breeding mode of litopenaeus vannamei, obtain high breeding yield of litopenaeus vannamei, and the survival rate of the litopenaeus vannamei fry "Bohai No.1" is 85.8-93.3% on average after 90 days of breeding, and the average weight of the individual is 11.6-14.9g.

[0021] The present application successfully applies the bio-floc to the factory breeding mode of litopenaeus vannamei, realizes high breeding yield, and improves the diversification of shrimp breeding in China.

[0022] In the establishment of the breeding system, the bio-floc cultivated by the purple non-sulfur bacteria and the nitrosomonas, combined with the breeding of the perciformes, regulates the pH value and the C / N value of the water body, can continuously and stably establish a micro food web, promote the nutrient cycle of the breeding water body, and provide natural bait for the shrimp, improve the feed utilization rate, provide antibacterial activity for the shrimp, and the bio-floc can also purify the water quality, so that the water can not be changed during the breeding process, effectively avoiding the introduction of exogenous pathogens, and reducing the production cost. DETAILED DESCRIPTION

[0023] In order to better understand the technical content of the present application, the following specific examples are provided to further illustrate the present application.

[0024] The experimental methods used in the embodiments of the present application are conventional methods unless otherwise specified.

[0025] The materials, reagents and the like used in the embodiments of the present application can be obtained from commercial channels unless otherwise specified.

[0026] Example 1 - A factory domestication method of Litopenaeus vannamei "Bohai No. 1" fry

[0027] S1 Pre-treatment of the culture pond: the water body of the culture pond is set to 35 m 3 , the bottom mud is laid on the bottom of the culture pond, 28 air stones with a mesh size of 120 are placed on the bottom mud, the culture pond is disinfected with potassium permanganate before use, the culture water is filtered through a sand filter and then enters a sedimentation tank for sedimentation, after being disinfected with bleaching powder for 24 hours, aeration and sedimentation are carried out, and the residual chlorine is naturally disappeared, the water is filtered and injected into the culture pond, and the water quality of the water source should meet the requirements of GB 11607-89;

[0028] S2 Establishment of the culture system:

[0029] Method one: inoculating biological floc in the culture pond, the concentration of the biological floc is 45 mL / L, the pH value of the water body is adjusted to 8.6-9.0, and the C / N value is 15-20;

[0030] After inoculating the biological floc for 2 days, tilapia is put into the culture pond for breeding, the density of the tilapia is 120 tails per mu, the average weight of each tilapia is about 200-250 g, the pH value of the water body is adjusted to 7.4-7.6, and the C / N value is 10-15;

[0031] Through inoculation of the biological floc and breeding of the tilapia, a stable biological floc culture system is established after 18 days of cultivation.

[0032] Method two: inoculating biological floc in the culture pond, the concentration of the biological floc is 45 mL / L, after inoculating the biological floc for 2 days, the density of the tilapia is 120 tails per mu, the tilapia is put into the culture pond for breeding, the average weight of each tilapia is about 200-250 g, the pH value of the water body is adjusted to 8.6-9.0, and the C / N value is 15-20;

[0033] Through inoculation of the biological floc and breeding of the tilapia, a stable biological floc culture system is established after 18 days of cultivation.

[0034] Method three: inoculating biological floc in the culture pond, the concentration of the biological floc is 45 mL / L, after inoculating the biological floc for 2 days, the density of the tilapia is 120 tails per mu, the tilapia is put into the culture pond for breeding, and the average weight of each tilapia is about 200-250 g;

[0035] Through inoculation of the biological floc and breeding of the tilapia, a stable biological floc culture system is established after 18 days of cultivation.

[0036] The bio-floc cultivation of the above-mentioned method one, method two and method three is as follows: before use, the bio-floc cultivation tank is disinfected with potassium permanganate, 7 mL of complex bacterial inoculum and 1.2 mL of complex algal inoculum are injected into 1 L of sterile water, 2.5 g of glucose is added, after thorough mixing, aeration activation is performed for 10 min, 21 g of clay is added, and aeration activation is continuously performed until the bio-floc is formed;

[0037] The complex bacterial inoculum is 1 x 10 6 CFU / L of purple non-sulfur bacteria and 2 x 10 6 CFU / L of nitrosomonas, and the complex algal inoculum is 5 x 10 5 individuals of seawater chlorella and 1 x 10 5 individuals of nannochloropsis.

[0038] S3 domestication of seedling culture: the "Bohai No. 1" seedling is temporarily cultured, and is put into the culture tank at 0.8 ten thousand individuals / m 3 , and at least on the third day after the seedlings are put, artificial feed is fed, 3 times a day, 2-3% of the weight of the seedlings each time; during the period of thickening of the shell, the feed is fed 5 times a day, 2-3% of the weight of the seedlings each time;

[0039] The initial salinity of the water body is 30-33 salinity, the salinity of the water body is increased by 0.2-0.4 salinity per day until the salinity of the water body is 62-65 salinity, and the salinity of the water body is maintained at 62-65 salinity until the target prawns are harvested;

[0040] During the culture period, the water environment indexes are controlled: the transparency is 40-60 cm, the dissolved oxygen is more than 5 mg / L, and there is no nitrite.

[0041] Example 2 - a factory domestication method of white shrimp "Bohai No. 1" seedlings

[0042] S1 pre-treatment of culture tank: the water body of the culture tank is set to 35 m 3 , the bottom mud is laid on the bottom of the culture tank, 28 air stones with a mesh size of 120 are placed on the bottom mud, before use, the culture tank is disinfected with potassium permanganate, the culture water is filtered through a sand filter and then enters a sedimentation tank for sedimentation, after being disinfected with bleaching powder for 24 hours, aeration and sedimentation are performed, and the residual chlorine is naturally disappeared, the water is filtered and injected into the culture tank, and the water quality should meet the requirements of GB 11607-89;

[0043] S2 establishment of culture system: the culture tank is inoculated with bio-floc, the concentration of the bio-floc is 45 mL / L, the pH value of the water body is adjusted to 8.6-9.0, and the C / N value is 15-20;

[0044] After inoculating the bio-floc for 2 days, tilapia is put into the culture, the density is 120 per mu, the weight of each tilapia is about 200-250 g, the pH value of the water body is adjusted to 7.4-7.6, and the C / N value is 10-15;

[0045] After the culture of the perciformes fish for 4 days, the bio-floc is inoculated again, the concentration of the bio-floc is 22 mL / L, the pH value of the water body is adjusted to 7.8-8.2, and the C / N value is 8-10;

[0046] Through the inoculation of the bio-floc twice and the putting of the tilapia into the culture, the stable bio-floc culture system is established after 20 days of cultivation;

[0047] The bio-floc cultivation is as follows: before the use of the bio-floc cultivation pool, the pool is disinfected by potassium permanganate, sterile water is injected, 7 mL of compound bacterial seed liquid and 1.2 mL of compound algal liquid are inoculated in each 1 L of the sterile water, 2.5 g of glucose is added, after the thorough mixing, aeration activation is carried out for 10 min, 21 g of clay is added, and the aeration activation is continued until the bio-floc is formed;

[0048] The compound bacterial seed liquid is 1×10 6 CFU / L of purple non-sulfur bacteria and 2×10 6 CFU / L of nitrosomonas, the compound algal liquid is 5×10 5 individuals of marine chlorella and 1×10 5 individuals of nannochloropsis.

[0049] S3 fry culture domestication: the “Bohai No. 1” fry is temporarily cultured, and is put into the culture pond at 0.8 ten thousand per mu 3 , and after the fry is put, artificial feed is fed at least on the third day, 2-3% of the weight of the fry is fed 3 times a day; during the period of marking the body, 2-3% of the weight of the fry is fed 5 times a day.

[0050] The initial salinity of the water body is 30-33 salinity, the salinity of the water body is increased by 0.2-0.4 salinity per day until the salinity of the water body is 62-65 salinity, and the salinity of the water body is maintained at 62-65 salinity until the target prawns are harvested.

[0051] During the culture, the water environment indexes are controlled: the transparency is 40-60 cm, the dissolved oxygen is more than 5 mg / L, and there is no nitrite.

[0052] Example 3 - A factory domestication method of the Litopenaeus vannamei “Bohai No. 1” fry

[0053] S1 culture pond pretreatment: the water body of the culture pond is set to 35 m 3The bottom of the breeding pond is paved with bottom mud, 28 air stones with 120 meshes are placed on the bottom mud, the breeding pond is disinfected with potassium permanganate before use, the breeding water is filtered through a sand filter and then is deposited in a sedimentation tank, after being disinfected with bleaching powder for 24 hours, the water is aerated and deposited, and then is filtered and injected into the breeding pond after the residual chlorine naturally disappears, and the water quality should meet the requirements of GB 11607-89;

[0054] S2: Establishing a breeding system: inoculating biological flocs into the breeding pond, the concentration of the biological flocs is 48 mL / L, the pH value of the water body is adjusted to 8.6-9.0, and the C / N value is 15-20;

[0055] After 3 days of inoculation of the biological flocs, the perch is bred by being put into the breeding pond, the density of the perch is 150 per mu, the average weight of each tilapia is about 250-300 g, the pH value of the water body is adjusted to 7.4-7.6, and the C / N value is 10-15;

[0056] After 5 days of breeding of the perch, the biological flocs are inoculated again, the concentration of the biological flocs is 22 mL / L, the pH value of the water body is adjusted to 7.8-8.2, and the C / N value is 8-10;

[0057] Through the inoculation of the biological flocs twice and the breeding by putting in the tilapia, the stable biological floc breeding system is established after 20 days of cultivation;

[0058] The biological floc cultivation is as follows: the biological floc cultivation tank is disinfected with potassium permanganate before use, sterile water is injected, 5 mL of compound bacterial seed liquid and 0.5 mL of compound algal liquid are inoculated into each 1 L of the sterile water, 2 g of glucose is added, and after being mixed thoroughly, aeration is carried out for 10 min, 20 g of clay is added, and aeration is continuously carried out until the biological flocs are formed;

[0059] The compound bacterial seed liquid is 1×10 6 CFU / L of purple non-sulfur bacteria and 2×10 6 CFU / L of nitrosomonas, and the compound algal liquid is 5×10 5 individuals of marine chlorella and 1×10 5 individuals of parachlorella.

[0060] S3: Larvae breeding and domestication: the "Bohai No. 1" larvae are temporarily bred, and are put into the breeding pond at the density of 10,000 per mu 3 , and at least on the 3rd day after the larvae are put, artificial compound feed is fed, 2-3% of the weight of the larvae is fed 3 times a day; during the period of marking the body, 2-3% of the weight of the larvae is fed 5 times a day.

[0061] The initial salinity of the water body is 30-33 salinity, the salinity of the water body is increased by 0.2-0.4 salinity every day until the salinity of the water body is 62-65 salinity, and then the salinity of the water body is maintained at 62-65 salinity until the target prawns are harvested;

[0062] During the cultivation period, the water environment indicators are controlled as follows: the transparency is 40-60 cm, the dissolved oxygen is more than 5 mg / L, and there is no nitrite.

[0063] Comparative Example 1 - A factory domestication method of Litopenaeus vannamei "Bohai No. 1" seedlings

[0064] S1 Pretreatment of the cultivation pond: the water body of the cultivation pond is set to 35 m 3 Before use, the cultivation pond is disinfected with potassium permanganate, and the water for cultivation is filtered through a sand filter and then deposited in a sedimentation tank. After being disinfected with bleaching powder for 24 hours, the water is aerated and deposited, and then filtered and injected into the cultivation pond after the residual chlorine naturally disappears. The water quality should meet the requirements of GB 11607-89;

[0065] S2 Establishment of the cultivation system: the cultivation pond is inoculated with biological floc, and the concentration of the biological floc is 45 mL / L. The pH value of the water body is adjusted to 8.6-9.0, and the C / N value is 15-20. The biological floc is cultivated for 18 days to establish a stable biological floc cultivation system.

[0066] The biological floc cultivation is as follows: before use, the biological floc cultivation pond is disinfected with potassium permanganate, and sterile water is injected. 7 mL of compound bacterial seed liquid and 1.2 mL of compound algal liquid are inoculated into every 1 L of sterile water, 2.5 g of glucose is added, and then mixed thoroughly. After being aerated and activated for 10 minutes, 21 g of clay is added and continuously aerated and activated until the biological floc is formed.

[0067] The compound bacterial seed liquid is 1 × 10 6 CFU / L of purple non-sulfur bacteria and 2 × 10 6 CFU / L of nitrosomonas, and the compound algal liquid is 5 × 10 5 individuals of Chlorella marina and 1 × 10 5 individuals of Nannochloropsis.

[0068] S3 Seedling cultivation domestication: the "Bohai No. 1" seedlings are temporarily cultivated and then put into the cultivation pond at a rate of 0.8 ten thousand individuals / m 3 . At least on the third day after the seedlings are put in, artificial compound feed is fed, 3 times a day, at a rate of 2-3% of the weight of the seedlings. During the period of thickening the shell, the feed is fed 5 times a day, at a rate of 2-3% of the weight of the seedlings.

[0069] The initial salinity of the water body is 30-33 salinity, and the salinity of the water body is increased by 0.2-0.4 salinity every day until the salinity of the water body is 62-65 salinity. The salinity of the water body is then maintained at 62-65 salinity until the target prawns are harvested.

[0070] During the cultivation period, the water environment indicators are controlled as follows: the transparency is 40-60 cm, the dissolved oxygen is more than 5 mg / L, and there is no nitrite.

[0071] Comparative Example 2 - A factory domestication method of Penaeus vannamei "Bohai No. 1" fry

[0072] S1 Pre-treatment of the culture pond: The water body of the culture pond was set to 35 m 3 , The bottom of the culture pond was paved with mud, and 28 air stones with a mesh size of 120 were placed on the mud. Before use, the culture pond was disinfected with potassium permanganate. The water for culture was filtered through a sand filter and then deposited in a sedimentation tank. After 24 hours of bleaching powder disinfection treatment, aeration and sedimentation were performed. After the residual chlorine naturally disappeared, the water was filtered and injected into the culture pond. The water quality should meet the requirements of GB 11607-89;

[0073] S2 Establishment of the culture system: The culture pond was inoculated with biological floc, and the concentration of the biological floc was 45 mL / L. The pH value of the water body was adjusted to 8.6-9.0, and the C / N value was 15-20. The inoculation of the biological floc was cultured for 18 days to establish a stable biological floc culture system.

[0074] The biological floc culture was as follows: The biological floc culture pond was disinfected with potassium permanganate before use. Sterile water was injected, 7 mL of complex bacterial seed liquid was inoculated into every 1 L of sterile water, 2.5 g of glucose was added, and then it was mixed thoroughly. After 10 minutes of aeration and activation, 21 g of clay was added and the aeration and activation were continued until the biological floc was formed.

[0075] The complex bacterial seed liquid was 1 × 10 6 CFU / L of lactic acid bacteria and 2 × 10 6 CFU / L of Bacillus.

[0076] S3 Domestication of fry culture: The "Bohai No. 1" fry was temporarily cultured, and 0.8 million fry / m 3 were released into the culture pond. Artificial feed was fed at least 3 days after the release of the fry, 3 times a day, and 2-3% of the weight of the fry per time. During the period of marking the shell, the feed was fed 5 times a day, and 2-3% of the weight of the fry per time.

[0077] The initial salinity of the water body was 30-33 salinity. The salinity of the water body was increased by 0.2-0.4 salinity per day until the salinity of the water body was 62-65 salinity. The salinity of the water body was then maintained at 62-65 salinity until the target prawns were harvested.

[0078] During the culture period, the water environment indicators were controlled as follows: the transparency was 40-60 cm, the dissolved oxygen was more than 5 mg / L, and there was no nitrite.

[0079] In 2020-2021, a culture pond in Wenchang City, Wengtian Town, Hainan Province was used for prawn culture test starting from June to July, and the results are shown in Table 1 below.

[0080] Table 1 survival rate and body weight determination results of Litopenaeus vannamei "Bohai No. 1" fry culture for 90d

[0081] Item Method 1 Method 2 Method 3 Example 2 Comparative Example 1 Comparative Example 2 Survival % 92.6 88.7 85.8 93.3 80.1 72.5 Body weight g / fish 12.4 12.0 11.6 14.9 11.3 10.6

[0082] The determination results show that in the culture mode of the culture pond, the survival rate of Litopenaeus vannamei "Bohai No. 1" is 85.8-93.3%, and the average individual weight at harvest is 11.6-14.9g, and the establishment of a stable biological floc culture system can realize a higher survival rate and weight in the culture mode of the culture pond.

[0083] Example 2, compared with Comparative Examples 1 and 2, in the establishment of the culture system, the biological floc cultivated from purple non-sulfur bacteria and nitrosomonas, combined with the culture of Perciformes fish, regulates the pH value and C / N value of the water body, can continuously and stably establish a micro food web, promote the nutrient cycle of the culture water body, help to improve the survival rate of Litopenaeus vannamei, and promote its growth and development.

[0084] Determination of antibacterial activity: on the 60th and 90th day of culture, 100 shrimps were randomly selected from each pond, starved for 24h, and the heart blood was drawn and placed in a 4℃ refrigerator until the serum was blue.

[0085] The E. coli was activated and cultured to the logarithmic phase, and the bacterial number was adjusted to 1×10 6 CFU / L, 3mL of bacterial solution was taken, ice bath, 0.2mL of shrimp serum was added, vortexed, 37℃ reaction for 30min, placed on ice bath for 1min, shrimp serum was blank, and the absorbance value was measured at 570nm.

[0086] Calculation formula: antibacterial activity U = [(A0-A1) / A1] 1 / 2 , wherein A0 is the blank absorbance value, and A1 is the test absorbance value, and the determination results are as shown in Table 2.

[0087] Table 2 determination results of antibacterial activity of Litopenaeus vannamei "Bohai No. 1" fry culture for 60d and 90d

[0088] Item Method 1 Example 2 Comparative Example 2 60d 0.37 0.40 0.35 90d 0.41 0.48 0.37

[0089] The determination results show that in the culture mode of the culture pond, the antibacterial activity of Litopenaeus vannamei "Bohai No. 1" is improved to varying degrees with the extension of the culture time, and the establishment of a stable biological floc culture system can realize the ability to resist E. coli and adversity stress in the culture mode of the culture pond.

[0090] The above only describes the preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A method for factory domestication of Litopenaeus vannamei "Bohai No. 1" fry, characterized in that, The method comprises the following steps: S1: Pre-treatment of the culture pond: disinfect the culture pond and the water used for culture, and lay the bottom mud on the bottom of the culture pond; S2: Establishing the culture system: inoculate the biological floc into the culture pond, the concentration of the biological floc is 42-48 mL / L, inoculate the biological floc for at least 2 days, and then put the perciform fish into the culture pond to establish the biological floc culture system; the biological floc is cultivated by purple non-sulfur bacteria and nitrosomonas; the perciform fish is tilapia or sea bass; the density of the perciform fish is 100-150 per mu, and the weight is 200-300 g; In step S2, after the culture pond is inoculated with the biological floc, the pH value of the water body is adjusted to 8.6-9.0, and the C / N value is adjusted to 15-20 before the perciform fish is put into the culture pond for culture; after the perciform fish is put into the culture pond for culture, the pH value of the water body is adjusted to 7.4-7.6, and the C / N value is adjusted to 10-15; The preparation method of the biological floc is as follows: 5-8 mL of the compound bacterial inoculum and 0.5-2 mL of the compound algal inoculum are inoculated into 1 L of sterile water, 2-3 g of glucose is added, mixed, aerated and activated, 20-22 g of clay is added, and the aeration and activation are continued to obtain the biological floc; the compound bacterial inoculum is purple non-sulfur bacteria and nitrosomonas, and the compound algal inoculum is marine chlorella and nannochloropsis; In step S2, the cultivation time for establishing the biological floc culture system is at least 18 days; S3 seed cultivation domestication: "Bohai No. 1" seedling temporary breeding, 0.8-1 million tail / m 3 , put into the breeding pond; the initial salinity of the water body is 30-33 salinity, the salinity of the water body is increased by 0.2-0.4 salinity per day, until the salinity of the water body is 62-65 salinity, and then the salinity of the water body is maintained at 62-65 salinity until the target prawns are harvested.

2. The factory domestication method of the Litopenaeus vannamei "Bohai No. 1" seed according to claim 1, characterized in that, The perciform fish is cultured for at least 4 days, and the method further comprises re-inoculating the biological floc, and the concentration of the biological floc is 20-25 mL / L.

3. The factory domestication method of the Litopenaeus vannamei "Bohai No. 1" seed according to claim 2, characterized in that, After the biological floc is re-inoculated, the pH value of the water body is adjusted to 7.8-8.2, and the C / N value is adjusted to 8-10.

4. The factory domestication method of the Litopenaeus vannamei "Bohai No. 1" seed according to claim 1, characterized in that, In step S3, the artificial compound bait is fed to the perciform fish at least on the third day after the fry is put into the culture pond, the artificial compound bait is fed 3-5 times a day, and the weight of the fry is 2-3% of the weight of the artificial compound bait.