A kind of wakame seed line, a kind of wakame seed line manufacturing method and a kind of wakame breeding method using wakame seed line

Abstract

The present invention relates to a sea spaghetti seed line, a method for manufacturing the sea spaghetti seed line, and a method for culturing sea spaghetti using the sea spaghetti seed line. The present invention provides a sea spaghetti seed line that controls the growth and development density of sea spaghetti on the sea spaghetti seed line, and is suitable for a method (winding method) in which the sea spaghetti seed line is wound and installed on a main rope. A sea spaghetti seed line for sea spaghetti culture, characterized by having a coated portion in which a dry oil is attached to a line-shaped epiphytic substrate, and a non-coated portion in which no dry oil is attached.

Description

TECHNICAL FIELD

[0001] The present application relates to a sea spaghetti seed line, a method for manufacturing the sea spaghetti seed line, and a method for culturing sea spaghetti using the sea spaghetti seed line. BACKGROUND

[0002] Conventionally, about 95% of sea spaghetti production in Japan is performed by harvesting cultured sea spaghetti. In addition, general production of cultured sea spaghetti is performed by the following processes.

[0003] (1) A process of making sea spaghetti seed lines by attaching sea spaghetti spores to a thread-like periphytic substrate.

[0004] (2) A process of growing the sea spaghetti spores attached to the seed lines into young leaves by raising seedlings, temporary cultivation, and the like.

[0005] (3) A process of installing the seed lines to which young leaves are attached (hereinafter also referred to as "seedling lines") on a main rope.

[0006] (4) A process of suspending the main rope on which the seed lines to which young leaves are attached are installed on a raft or the like and growing the young leaves in the sea or the like to form sea spaghetti.

[0007] (5) A process of harvesting the grown sea spaghetti from the main rope.

[0008] In the above process (3), a method of cutting the seed lines to which young leaves are attached into a moderate length and inserting the obtained seed lines into the main rope at a moderate interval (insertion method) or a method of winding the seed lines to which young leaves are attached on the main rope (winding method) or the like is performed.

[0009] In this case, in the insertion method, there is a trouble of cutting the seed lines to which young leaves are attached (seedling lines) into a moderate length and further inserting them into the main rope at a moderate interval or the like. In addition, in the case where the inserted seedlings are detached due to the influence of waves or the seedling lines used in the insertion do not have a sufficient number of young leaves, a state of "abscission" that is not suitable for cultivation is caused, the work is troublesome, and there are problems such as a risk of failure in cultivation.

[0010] On the other hand, the entwining method is a useful method in which the risk of the detachment of the seed line like the above-mentioned pinching method, the insufficient number of young leaves is very low, and thus is adopted particularly in the cultivation site of Undaria pinnatifida where the influence of waves is significant, such as Iwate Prefecture and the like. However, on the Undaria pinnatifida seed line obtained in the above-mentioned step (1), Undaria pinnatifida spores are uniformly attached to the epiphytic substrate, and thus, in the above-mentioned step (4), the growth and development density of Undaria pinnatifida becomes high during the growth of Undaria pinnatifida, and sometimes, the growth of Undaria pinnatifida is hindered due to the poor photosynthesis, the insufficient nutrition, and the like, or the detachment due to the insufficient survival of the rhizoid portion on the epiphytic substrate occurs due to the excessively high growth and development density. Thus, there is a trouble of lifting the main rope from the sea and the like in the middle of the step (4) and thinning a part of Undaria pinnatifida grown. The thinning operation is most preferable from the experience that it is performed before the full length of Undaria pinnatifida reaches 1 m, that is, at the period when the full length is about 30 to 70 cm, but the situation that the operation cannot be performed in the early January of the previous year due to the influence of waves, cold current, and the like is more in the period, and becomes a large operation burden in a series of Undaria pinnatifida cultivation operation steps, and becomes a main factor of reducing the productivity.

[0011] Thus, whichever method is adopted, there is a problem that a large burden is generated for the person who engages in the cultivation of Undaria pinnatifida.

[0012] As the related art relating to the Undaria pinnatifida seed line for the cultivation of Undaria pinnatifida, there are disclosed: a culture tool which is a culture tool for culturing spores of algae and growing, characterized by being composed of a seed line in which spores of algae are attached and developed, and a frame body provided in a manner capable of mounting the seed line, the frame body having an upper frame material and a lower frame material disposed at a position apart by a predetermined distance and capable of hanging the seed line, and a pair of side frame materials detachably coupled with respect to the upper and lower frame materials (Patent Literature 1); a method for producing seaweed seedlings, characterized by disposing a collective support bar body in which a plurality of support bar bodies composed of a rope cut to a predetermined length are integrated in a flat plate shape in parallel in seawater, and causing reproductive cells and the like of seaweed to attach to the surface thereof, growing the epiphytic seaweed seedlings on the collective support bar body to an appropriate size, and separating the collective support bar body into each support bar body to which the seaweed seedlings are attached (Patent Literature 2); an Undaria pinnatifida seed line in which a Cremona line of a synthetic fiber is wound on a 30 cm square seedling frame of a wire as a seed frame (Non-Patent Literature 1); an Undaria pinnatifida seed line in which a Cremona line of a synthetic fiber is wound on a frame of PVC after growing and developing spores into a gametophyte (Non-Patent Literature 2, Non-Patent Literature 3), and the like.

[0013] However, in the technologies described in Patent Documents 1, 2 and Non-Patent Documents 1 to 3, since spores adhere to the entire surface of the thread portion of the Undaria pinnatifida seed line, there is a disadvantage that the growth density is too high when the Undaria pinnatifida grows, and an Undaria pinnatifida seed line suitable for the winding method has not been proposed.

[0014] Prior Art Documents

[0015] Patent Documents

[0016] Patent Document 1: Japanese Patent Application Laid-Open No. 2001-231387

[0017] Patent Document 2: Japanese Patent Application Laid-Open No. 2006-42687

[0018] Non-Patent Documents

[0019] Non-Patent Document 1: Dejima Prefecture Fisheries Research Report No. 10

[0020] Non-Patent Document 2: JAT AFF JOURNAL Vol. 9, No. 3 (March 1, 2021)

[0021] Non-Patent Document 3: JOURNAL OF APPLIED PHYCOLOGY 29(3) 1429-1436 (June 2017) SUMMARY

[0022] Problems to be Solved by the Invention

[0023] An object of the present application is to provide an Undaria pinnatifida seed line, a manufacturing method of the Undaria pinnatifida seed line, and a cultivation method of Undaria pinnatifida using the Undaria pinnatifida seed line, which controls the growth density of Undaria pinnatifida on the Undaria pinnatifida seed line and is suitable for a method (winding method) in which the Undaria pinnatifida seed line is wound and mounted on a main rope.

[0024] Method for Solving the Problem

[0025] The present inventors have conducted intensive research in order to solve the above problem, and as a result, have found that the above problem is solved by providing a coated portion formed of a drying oil to a certain portion of a thread-like epiphytic substrate used in the Undaria pinnatifida seed line. The present inventors have further conducted research based on these insights, and thus completed the present application.

[0026] That is, the present application includes the following configuration.

[0027] [1] An Undaria pinnatifida seed line, which is an Undaria pinnatifida seed line for Undaria pinnatifida cultivation, characterized by having a coated portion in which a drying oil is attached and a non-coated portion in which no drying oil is attached on a thread-like epiphytic substrate.

[0028] [2] A method for manufacturing a sea spaghetti seed line, which is a method for manufacturing a sea spaghetti seed line used for sea spaghetti cultivation, characterized by providing a coated portion formed by providing a dry oil on a thread-like substratum and a non-coated portion to which no dry oil is provided, and by bringing the thread-like substratum into contact with a solution containing sea spaghetti spores, so that the sea spaghetti spores are mainly attached to the non-coated portion.

[0029] [3] A method for cultivating sea spaghetti, characterized by installing the sea spaghetti seed line described in [1] on a main rope in a roll-in manner, and growing young leaves in seawater to form sea spaghetti, and harvesting the grown sea spaghetti from the main rope.

[0030] The seawater is not particularly limited, and can be seawater present in the sea, seawater directly collected from the sea, underground seawater collected from the ground or the seabed (e.g., continental shelf) near the coast, ocean deep water collected from the deep sea, or the like. In addition, water having the same composition as seawater, which is formed by adding necessary mineral components (e.g., salts such as calcium, potassium, sodium, magnesium, and the like) to distilled water, tap water, or the like, can also be used. Note that the salt concentration of general seawater is usually 2.8 to 3.5 mass%, and the mineral component concentration is usually 1.0 to 1.6 mass%.

[0031] The nutrient conditions of the seawater are not particularly limited, but with respect to the concentration of nitrate nitrogen, which is a main nutrient salt component for seaweed such as sea spaghetti, for example, it is preferable to contain 0.1 to 50 μmol / L, more preferable to contain 0.2 to 10 μmol / L, and further preferable to contain 0.5 to 10 μmol / L. In addition, with respect to the concentration of phosphate, which is another nutrient salt component for seaweed such as sea spaghetti, for example, it is preferable to contain 0.01 to 5 μmol / L, more preferable to contain 0.02 to 1 μmol / L, and further preferable to contain 0.05 to 1 μmol / L.

[0032] Effects of the Invention

[0033] The sea spaghetti seed line of the present application exerts the following effects: sea spaghetti spores are difficult to attach to the coated portion of the thread-like substratum formed by the dry oil, and are attached to the non-coated portion to which no dry oil is provided to grow and develop.

[0034] The sea spaghetti seed line of the present application can control the sea spaghetti growth and development density because it has such a coated portion and a non-coated portion. Therefore, in the case where the sea spaghetti seed line of the present application is installed on a main rope in a roll-in manner to cultivate sea spaghetti, the sea spaghetti growth and development density can be appropriately maintained, and thus it is expected that there is no need for or reduction in the trouble of thinning and the like.

[0035] Further, since seaweed other than wakame, diatoms, and the like also hardly adhere to the coated portion, wakame that is attached to the uncoated portion does not compete for environmental resources such as light and nutrient salts with seaweed other than wakame, diatoms, and the like, and growth can be expected to be promoted. DETAILED DESCRIPTION

[0036] The wakame seed line of the present application is a wakame seed line in which a coated portion in which a dry oil is attached and an uncoated portion in which a dry oil is not attached exist on the surface portion of a line-shaped attached substrate, and wakame spores mainly adhere to the uncoated portion. In addition, the wakame seed line of the present application also includes a wakame seed line (seedling line) in which young leaves that have grown from the above-mentioned wakame spores are attached.

[0037] The line-shaped attached substrate used in the present application is not particularly limited as long as it is a line-shaped material and shape to which wakame spores can adhere, and is a line-shaped attached substrate used in seed lines for wakame cultivation in general. As a specific material, for example, palm materials, natural fibers such as kapok, Cremona (registered trademark), chemical fibers such as polyester, and the like can be listed. As a specific shape, for example, a shape having a diameter of about 1 to 3 mm, a line-shaped shape in which a plurality of fibers are twisted, and the like can be listed.

[0038] The dry oil used in the present application is not particularly limited as long as it is a dry oil that is cured by oxidation in air, and for example, an oil and fat having an iodine value of 130 or more is preferred. As a specific dry oil, for example, linseed oil, tung oil, mustard oil, perilla oil, walnut oil, sesame oil, soybean oil, safflower oil, sunflower oil, castor oil, grape seed oil, and the like can be listed, and linseed oil is preferred. One or two or more kinds of these dry oils can be used.

[0039] In the dry oil used in the present application, an iron salt having an effect of promoting curing of the dry oil is preferably used in combination. As the above-mentioned iron salt, for example, ferrous chloride, ferric chloride, and the like can be listed. In the case where an iron salt is used in combination, as the amount of addition of the iron salt, the amount of iron ions in the iron salt is preferably 0.001 to 0.1 parts by mass, and more preferably 0.005 to 0.03 parts by mass, with respect to 100 parts by mass of the dry oil.

[0040] In order to easily control the attachment state of wakame spores on the surface portion of the line-shaped attached substrate and the range (length) of the coated portion in which the dry oil is attached, the dry oil used in the present application is preferably a dry oil to which tackiness is imparted. As a method of imparting tackiness to the dry oil, for example, a method of lowering the temperature of the dry oil; a method of using a solid particle network type oil and fat curing agent (hereinafter referred to as "solid particle type base material"); a method of using a molecular aggregate network type oil and fat curing agent (hereinafter referred to as "molecular aggregate type base material"); and the like can be listed.

[0041] As the solid particle type base material, polyglycerin fatty acid ester and the like can be exemplified, and a method in which about 1 mass% of polyglycerin fatty acid ester is mixed in the drying oil to impart viscosity can be exemplified.

[0042] As the molecular aggregate type base material, lecithin, sorbitan fatty acid ester and the like can be exemplified, and a method in which about 1 mass% of lecithin, sorbitan fatty acid ester, respectively, is mixed in the drying oil to impart viscosity can be exemplified.

[0043] The Undaria pinnatifida seed line used in the present application has a coated portion in which the drying oil is attached to the linear periphyton substrate, and a non-coated portion in which the drying oil is not attached, as described above. The coated portion refers to a state in which a cured product of the drying oil covers at least a surface portion of the linear periphyton substrate in a film shape. The coated portion has a property that Undaria pinnatifida spores are difficult to attach.

[0044] In the non-coated portion, the drying oil is not attached, and thus a cured product of the drying oil does not cover the surface portion of the linear periphyton substrate in a film shape. The non-coated portion is a portion in which Undaria pinnatifida spores attach and grow and develop Undaria pinnatifida.

[0045] The method of producing the coated portion is not particularly limited, and a method in which the drying oil is attached by contact treatment of at least a surface of the linear periphyton substrate and then the drying oil is cured can be exemplified. The method of curing the drying oil is not particularly limited, and a method in which the drying oil is left in the air to cure can be exemplified. As the time of leaving in the air, 48 hours or more, preferably 72 hours or more, can be exemplified, for example, in terms of the time of irradiation with direct sunlight. As the method of contact treatment of the drying oil, a method such as immersion, coating, spraying, and the like can be exemplified.

[0046] The balance between the coated portion in which the drying oil is attached to the surface portion of the linear periphyton substrate and the non-coated portion in which the drying oil is not attached is not particularly limited, and in terms of controlling the growth and development density of Undaria pinnatifida, it is effective that the coated portion and the non-coated portion exist alternately.

[0047] The length of the coated portion in which the drying oil is attached to the surface portion of the linear periphyton substrate and the non-coated portion in which the drying oil is not attached is not particularly limited, and in the case where the coated portion and the non-coated portion exist alternately, the coated portion is preferably 5 to 50 cm, more preferably 10 to 20 cm, and the non-coated portion is preferably 3 to 50 cm, more preferably 3 to 10 cm. If the above length, it is possible to control the growth and development density of Undaria pinnatifida, and thus it is preferable.

[0048] As the Undaria pinnatifida spores attached to the Undaria pinnatifida seed line, only Undaria pinnatifida spores used in existing Undaria pinnatifida cultivation can be used, and Undaria pinnatifida gametophytes, Undaria pinnatifida zoospores, and the like can be exemplified.

[0049] The method of attaching Undaria pinnatifida spores to the linear attachment substrate is not particularly limited, and examples that can be given include: a method in which the linear attachment substrate is immersed in a solution containing Undaria pinnatifida spores; a method in which the spores are attached by applying or spraying a solution containing Undaria pinnatifida spores to the linear attachment substrate; and the like. The number of Undaria pinnatifida spores attached to the linear attachment substrate is not particularly limited, provided that the number of spores attached is sufficient to enable cultivation of Undaria pinnatifida, and is preferably, for example, 5 to 20 per 10 cm, more preferably 8 to 12 per 10 cm, taking into account the harvest amount of Undaria pinnatifida, the quality of Undaria pinnatifida (the shape of the algal body, the presence or absence of folds on the surface of the leaf body, and the like), and the like. If the number of spores is within the above range, Undaria pinnatifida is prevented from covering each other, growth in the longitudinal direction is promoted, and Undaria pinnatifida grows longer, and furthermore, Undaria pinnatifida grown in this way has a shape in which the surface of the leaf body has few folds, and is therefore preferred.

[0050] Next, the method of producing the Undaria pinnatifida seed line according to the present application will be described, but the method of producing the Undaria pinnatifida seed line is also one embodiment of the present application. The method of producing the Undaria pinnatifida seed line is obtained by at least going through the following processes 1 and 2.

[0051] [Process 1]

[0052] A process of producing the "coated portion in which at least the surface portion of the linear attachment substrate is immersed in drying oil, the surface portion of the linear attachment substrate is coated or sprayed with drying oil, or the like, and the drying oil attached or impregnated is cured, and the drying oil is attached", and the "non-coated portion in which the contact treatment is not performed and the drying oil is not attached", and obtaining the above linear attachment substrate having the coated portion and the non-coated portion.

[0053] [Process 2]

[0054] A process of bringing the linear attachment substrate having the coated portion in which the drying oil is attached and the non-coated portion in which the drying oil is not attached, obtained in Process 1, into contact with a solution containing Undaria pinnatifida spores, and performing the contact treatment by, for example, immersion, coating, spraying, or the like, and causing the Undaria pinnatifida spores to mainly attach to the non-coated portion, thereby obtaining the Undaria pinnatifida seed line.

[0055] From the viewpoint of easily controlling the range of the coated portion of the drying oil on the attachment substrate, the drying oil used in the above Process 1 is preferably a drying oil to which a viscosity is imparted. Specifically, a method in which about 1 mass% of the above solid particle type base material or molecular aggregate type base material is mixed in advance in the drying oil and stirred, and direct sunlight is irradiated in the air for about 24 hours, thereby imparting a viscosity to the drying oil, can be given.

[0056] The wakame seed line obtained in the above-mentioned process 2 is preferably further subjected to seedling raising, temporary cultivation, and the like to grow the wakame spores attached to the seed line into young leaves. As the above-mentioned seedling raising and temporary cultivation, the method of seedling raising and the method of temporary cultivation that have been conventionally performed using the wakame seed line for wakame cultivation can be employed.

[0057] The wakame seed line thus obtained is wound and mounted on a main rope for wakame cultivation. A method of cultivating wakame in which the wakame seed line of the present application is wound and mounted on a main rope is also one of the modes of the present application.

[0058] The method of mounting the wakame seed line on the main rope is not particularly limited and the conventional method performed in wakame cultivation can be employed. For example, the following method can be cited: the wakame seed line is wound in advance on a cylindrical winding board and the wakame seed line is wound from the winding board on the main rope, and every 5 m or so, the wakame seed line is fixed with a binding tape or a raw material of a thread. At this time, the length of the wakame seed line wound on the main rope is not particularly limited and, for example, the wakame seed line is preferably 100 to 250 m per 100 m of the main rope, more preferably 150 to 200 m per 100 m of the main rope.

[0059] As the main rope on which the wakame seed line of the present application is wound, a main rope generally used in wakame cultivation can be used and the material and the shape are not particularly limited. As a specific material, for example, a chemical fiber such as polyester, vinylon, or the like can be cited. As a specific shape, for example, a rope-like shape having a diameter of about 20 to 50 mm can be cited, and the length varies depending on the cultivation fishing ground but generally a length of about 50 m to about 200 m can be cited. In addition, a main rope equipped with a steel wire in the center of the above-mentioned rope-like main rope can also be used.

[0060] The obtained main rope on which the wakame seed line is mounted can be used by the method employed in the conventional wakame cultivation. For example, one end of the main rope on which the wakame seed line is mounted is tied to a rope extending from a fixed device provided on the sea floor of a wakame cultivation fishing ground to the water surface, and the other end is tied to another rope extending from the fixed device to the water surface, so that the main rope is horizontally laid on the sea surface with the length of the main rope. At this time, a method of arranging the main rope on which the wakame seed line is mounted with a float so as to be arranged at a water depth of 1 to 2 m from the water surface or the like can be employed.

[0061] As described above, the main rope on which the wakame seed line is mounted, which is arranged in a wakame cultivation fishing ground, can cultivate wakame by the method employed in the conventional wakame cultivation. For example, a method in which wakame is generally cultivated during a period when the water temperature is lower than about 18°C, and if it is the Sanriku coast, the cultivation is generally started from the middle of November to the beginning of December, and from the end of March to the beginning of April after about 3 months or more, wakame is harvested by hand work by harvesting the wakame.

[0062] In the case of the existing cultivation method of wakame using a main rope on which wakame seed lines are installed, at the time when the full length of wakame reaches about 70 cm, which is generally in the first half of January if it is the Sanriku coast, short individuals with a full length of less than about 30 cm are harvested one by one, and the number of wakame per 1 m of the main rope is adjusted in density to 80 to 120, which is the optimum condition for maximizing the amount of harvest thereafter. In addition, at this time, for the purpose of securing a portion where the main rope is held by hand in the harvest thereafter, or for the purpose of securing a portion where a hook hand portion for hoisting the main rope using a crane installed on a boat is provided, and for the purpose of improving the flow of seawater with respect to the wakame algal body, an operation of leaving a portion to which wakame is attached as a stock of about 3 cm to about 30 cm and removing the rest is performed. However, for this period, the period of poor weather in previous years is many, and it becomes a great operation load for fishermen due to waves and cold currents. In addition, this operation requires long-term skilled techniques, and becomes a great hindrance to the improvement of the operability of new entrants into wakame cultivation. By using the wakame seed line of the present application, this density adjustment operation can be omitted, and a great improvement in operability can be achieved compared to the past.

[0063] Wakame cultivated by the wakame cultivation method using the wakame seed line of the present application can be harvested by the existing method, and further can be processed by the existing method. The harvested raw wakame can be made into processed products such as boiled frozen wakame, boiled salted wakame, and the like by performing existing processes such as blanching, freezing, salting, and the like, and further, by performing processes such as washing, cutting, drying, and the like on the boiled salted wakame and the like, processed products such as cut dried wakame and the like can be made.

[0064] As described above, by cultivating wakame using the wakame seed line of the present application, the density adjustment operation performed when cultivating using the wakame seed line in the past is not required, and even if this operation is not performed, wakame is uniformly affected by waves and growth is promoted, and further, leafy quality with a thick flesh is formed, and further, a homogeneous shape can be expected. The growth and the homogeneous shape can also contribute to improving the operability at the time of harvesting.

[0065] Hereinafter, the present application will be described using examples, but this is simply to illustrate the present application and is not to limit the present application.

[0066] Example

[0067] 1. Production of wakame seed line and evaluation of attachment condition of wakame gametophytes

[0068] (1) Production of wakame seed line

[0069] The Cremora thread (trade name: manufactured by the First Net Co., Ltd., diameter 2 mm, length 10 m) as the linear adherend substrate was dipped in the oil such as drying oil shown in the following [coating material] every 50 cm for 10 minutes, and then, left for 72 hours under direct sunlight irradiation in a room at normal temperature (about 25°C) in terms of the time of direct sunlight irradiation to produce the Cremora threads 1 to 6 having a coated portion and a non-coated portion.

[0070] The Cremora threads 1 to 6 obtained having the coated portion and the non-coated portion were uniformly coated with the solution containing the Undaria pinnatifida spores obtained in the following [production 1 of solution containing Undaria pinnatifida spores] using a brush to produce the prototypes 1 to 6 as the Undaria pinnatifida seed threads.

[0071] In addition, without performing the dipping in the oil described above, the Cremora thread alone as the Cremora thread not having the coated portion was uniformly coated with the solution containing the Undaria pinnatifida spores obtained in the following [production 1 of solution containing Undaria pinnatifida spores] using a brush to produce the prototype 7 as the Undaria pinnatifida seed thread.

[0072] Here, the prototypes 1 to 3 correspond to the examples having the coated portion in which the drying oil is attached, the prototypes 4 to 6 correspond to the comparative examples having the coated portion in which the oil other than the drying oil is attached, and the prototype 7 corresponds to the reference example not having the coated portion.

[0073] [Coating material]

[0074] Coating material 1: Linseed oil (trade name: Linseed oil; manufactured by Nisshin Oil Mills, Ltd.) 500 g

[0075] Coating material 2: a solution obtained by mixing linseed oil (trade name: Linseed oil; manufactured by Nisshin Oil Mills, Ltd.) 500 g and ferrous chloride (trade name: Iron (II) chloride anhydrous; manufactured by FUJIFILM Wako Pure Chemical Corporation) 0.1 g

[0076] Coating material 3: Linseed oil (trade name: Linseed oil; manufactured by Horie Industrial Co., Ltd.) 500 g

[0077] Coating material 4: Rapeseed oil (trade name: Rapeseed oil; manufactured by Boso Oil & Fat Co., Ltd.) 500 g

[0078] Coating material 5: a substance obtained by heating and dissolving palm hardened oil (trade name: Palm super-hardened oil; manufactured by Yokokawa Oil & Fat Co., Ltd.) 500 g

[0079] Coating material 6: a substance obtained by heating and dissolving candelilla wax (trade name: Purified candelilla wax MK-2; manufactured by Yokokawa Oil & Fat Co., Ltd.) 500 g

[0080] [Production 1 of solution containing Undaria pinnatifida spores]

[0081] The female 0.0236 g and the male 0.0133 g of Undaria pinnatifida gametophytes (Iwate Prefecture stock) were added to sterilized seawater 20 g, and after a crushing treatment was performed using a homogenizer (Model: Pro 200 Homogenizer; manufactured by Pro Scientific) at 5000 rpm for 2 minutes, sterilized seawater 180 g was added to obtain about 200 g of a solution containing Undaria pinnatifida spores. The obtained solution containing spores was confirmed using an inverted microscope (magnification 40 times), and as a result, the number of cells of the crushed female and male spores was 5 to 10 cells.

[0082] (2) Culturing of Undaria pinnatifida seed lines

[0083] The Undaria pinnatifida seed lines (Prototype 1 to 7) were cultured in 1 / 4 PESI medium using an incubator (Model: CLE303; manufactured by TOMY KOGAKU) at 20°C, 2000 lux, 12 hours of light period, 12 hours of dark period, for 12 days.

[0084] (3) Evaluation of the attachment state of Undaria pinnatifida gametophytes to Undaria pinnatifida seed lines

[0085] Three portions each of the coated portion and the uncoated portion of Prototype 1 to 7, for which the culturing of the Undaria pinnatifida seed lines was completed, were cut out, and the number of Undaria pinnatifida spores that grew per 1 cm in each cut portion was counted in a field of view of an inverted microscope set to 8 times magnification, and the average of the obtained values was calculated as the number of Undaria pinnatifida gametophytes per 1 cm of the line, and the results were summarized in Table 1.

[0086] [Table 1]

[0087]

[0088] As is clear from the results shown in Table 1, for Prototype 1 to 3, which have a coated portion of linseed oil as a drying oil, the attachment of Undaria pinnatifida gametophytes to the coated portion is less, and the attachment of Undaria pinnatifida gametophytes to the uncoated portion is more. On the other hand, for Prototype 4 to 6, which have a coated portion of oil other than a drying oil, the attachment of Undaria pinnatifida gametophytes to the coated portion is more, and is equivalent to Prototype 7, which has no coated portion and only an uncoated portion.

[0089] Thus, in Undaria pinnatifida seed lines (Prototype 1 to 3) having a coated portion to which a drying oil is attached and an uncoated portion to which a drying oil is not attached, the portion to which Undaria pinnatifida gametophytes attach and the portion to which Undaria pinnatifida gametophytes do not easily attach can be clearly distinguished. By using Undaria pinnatifida seed lines obtained in this way wound around a main line, the portion in which Undaria pinnatifida spores attach and grow Undaria pinnatifida and the portion in which Undaria pinnatifida spores do not attach and do not grow Undaria pinnatifida exert the effect of having good results in the development of Undaria pinnatifida during Undaria pinnatifida cultivation, and the effect of not requiring thinning work during the middle of Undaria pinnatifida cultivation.

[0090] 2. Production of Undaria pinnatifida seed line and cultivation of Undaria pinnatifida and evaluation thereof

[0091] (1) Production of Undaria pinnatifida seed line

[0092] On a frame of 20 cm in length and 30 cm in width made of a polyvinyl chloride-made pipe (outer diameter 18 mm, inner diameter 13 mm), 80 m of Cremora thread (trade name: manufactured by the first net-making company, diameter 2 mm) as a thread-like periphyton substrate was wound at intervals of 3 mm, and a coating material obtained by mixing 0.1 g of ferrous chloride (trade name: anhydrous iron (II) chloride; manufactured by Fuji Photo Film and Otsuka Pharmaceutical Co., Ltd.) in 500 g of linseed oil (trade name: linseed oil; manufactured by Nippon Flour Mills Co., Ltd.) was applied to the Cremora thread using a brush in such a manner that 10 cm of the coated portion and 10 cm of the uncoated portion were alternated, and then the Cremora thread A having the coated and uncoated portions was left to stand in a room under direct sunlight at normal temperature (about 25°C) for 1 week to produce the Cremora thread A having the coated and uncoated portions.

[0093] The Cremora thread A having the coated and uncoated portions obtained was immersed in the solution containing Undaria pinnatifida spores obtained in [2. Production of solution containing Undaria pinnatifida spores] described below for 10 minutes, and then exposed to air for 1 minute to produce the Undaria pinnatifida seed line A in which the spores were promoted to survive on the thread.

[0094] In addition, the Cremora thread having no coated portion was treated in the same manner as the Undaria pinnatifida seed line A described above except that the application of the drying oil was not performed, and then immersed in the solution containing Undaria pinnatifida spores obtained in [2. Production of solution containing Undaria pinnatifida spores] described below for 10 minutes, and then exposed to air for 1 minute to produce the Undaria pinnatifida seed line B in which the spores were promoted to survive on the thread.

[0095] [2. Production of solution containing Undaria pinnatifida spores]

[0096] About 0.2 g of female and about 0.05 g of male Undaria pinnatifida gametophytes (Iwate Prefecture) were added to 20 g of sterilized seawater, and subjected to a crushing treatment using a homogenizer (model: Pro200 homogenizer; manufactured by Pro Scientific) at 5000 rpm for 2 minutes, and 1000 g of sterilized seawater was added to obtain about 1020 g of a solution containing Undaria pinnatifida spores. The solution containing Undaria pinnatifida spores obtained was confirmed using an inverted microscope (magnification 40 times), and as a result, the number of cells of the crushed spores of the female and the male was 5 to 10 cells.

[0097] (2) Cultivation of Undaria pinnatifida seed line

[0098] The Undaria pinnatifida seed lines A and B were immersed in the UV-sterilized seawater at 18°C and aerated under 100 lux for 45 days. Note that the seawater was replaced every 10 days, and 20 mL of Nori Seed (trade name: manufactured by the first net company) was added as a nutrient component per 100 L of seawater at the time of the replacement.

[0099] For the Undaria pinnatifida seed line A, which had completed the cultivation, it was confirmed that about 5 mm of the thalli, which had germinated from the Undaria pinnatifida spores, were attached to the non-coated portion of the dry oil at 30 to 100 per 1 cm of the seed line. For the Undaria pinnatifida seed line B, it was confirmed that about 5 mm of the thalli, which had germinated from the Undaria pinnatifida spores, were attached to the entire length at 30 to 100 per 1 cm of the seed line.

[0100] (3) Seawater cultivation of Undaria pinnatifida seed lines

[0101] The Undaria pinnatifida seed lines A and B were transported to the Undaria pinnatifida cultivation fishery in Osaki City, Miyagi Prefecture, and were vertically lowered at a water depth of 2 m for seawater cultivation. The frame was lifted every 3 to 5 days, and the operation of washing the seawater to remove the attached matter was performed, and seawater cultivation was performed for two weeks, and seed lines (seedling lines) in which the young leaves had grown to a total length of about 3 cm were obtained.

[0102] (4) Official cultivation of Undaria pinnatifida seed lines based on the roll-in method

[0103] The obtained seed lines (seedling lines) in which the young leaves had grown to a total length of about 3 cm were used to start official cultivation in the Kesennuma Bay in Iwate Prefecture from November.

[0104] The method of the official cultivation is generally to wind the Undaria pinnatifida seed lines (seedling lines) taken out from the frame around the cylindrical member made of polyurethane with a handle, which is used when the fisherman winds the Undaria pinnatifida seed lines around the main rope, and to install the Undaria pinnatifida seed lines (seedling lines) around the circumference of the main rope (100 m) for Undaria pinnatifida cultivation at a distance of about 1 km from the fishing port. At the time of the installation, the main rope and the Undaria pinnatifida seed lines (seedling lines) are fixed with a plastic tie at intervals of about 1 m. The winding portions of the Undaria pinnatifida seedling line A and the Undaria pinnatifida seedling line B are 80 m (80 m / main rope 50 m) with respect to the main rope 50 m, respectively. The main rope on which the Undaria pinnatifida seed lines are wound is disposed at a water depth of 1.5 m.

[0105] (5) Evaluation of the growth and development state of Undaria pinnatifida

[0106] In May of the second year, the main rope with the wakame seed line A wound thereon and the main rope with the wakame seed line B wound thereon were lifted up, and the growth and development of the wakame was evaluated by visual observation. As a result, in the case of the main rope with the wakame seed line A wound thereon, the wakame hardly grew and developed from the coated portion of the wakame seed line A, and the wakame grew and developed from the uncoated portion. On the other hand, in the case of the main rope with the wakame seed line B wound thereon, the wakame grew and developed from the entire wakame seed line B, and the wakame was in a dense state, and the length was short, the width was wide, and the individual weight (average weight per 1 plant) was light, and the wakame was in a poor growth and development state, as described later.

[0107] In addition, the main rope with the wakame seed line A wound thereon was divided into the coated portion in which the wakame hardly grew and developed and the uncoated portion in which the wakame grew and developed, and thus it was possible to ensure the portion to be gripped when the wakame was harvested by manual work or the portion of the crane provided on the harvester boat.

[0108] (6) Evaluation 1 of the harvested wakame

[0109] In order to find the total weight of the wakame per unit length of the main rope, the wakame grown and developed was harvested from the uncoated portion and the coated portion of the main rope with the wakame seed line A wound thereon, each 5 places (1 m each), and the false root portion as the attachment base was removed to obtain wakame A-1 (uncoated portion) and wakame A-2 (coated portion). The weight of the obtained wakame A-1, A-2 was measured, respectively, and the average weight of the wakame grown and developed per 1 m of the main rope was calculated. In addition, the wakame grown and developed was harvested from the main rope with the wakame seed line B wound thereon, each 5 places (1 m each), and the false root portion as the attachment base was removed to obtain wakame B-1. The weight of the obtained wakame B-1 was measured, and the average weight of the wakame grown and developed per 1 m of the main rope was calculated. The results are shown in Table 3.

[0110] (7) Evaluation 2 of the harvested wakame

[0111] In order to clarify the deviation among the individuals of the wakame, the wakame grown and developed per 1 m of the uncoated portion of the wakame seed line A and the wakame seed line B, respectively, was harvested, and the false root portion as the attachment base was removed to obtain 30 individuals (30 plants) of wakame A-3 (uncoated portion) and wakame B-2, which were the longest. The weight of the obtained 30 plants of wakame A-3, B-2 was measured, and the average weight per 1 plant was calculated. The results are shown in Table 3.

[0112] (8) Evaluation 3 of the harvested wakame

[0113] The obtained wakame leaf portion was boiled and salted by the following method, and the taste was evaluated by sensory evaluation.

[0114] [Production of boiled salted wakame]

[0115] After boiling the leafy part of the cultivated wakame in seawater heated to 90°C for 30 seconds, the boiled wakame was poured onto a colander, weighed after draining the water, and salted with about 40 mass% of salt based on the weight of the wakame for 24 hours. Then, the wakame was dehydrated to produce boiled salted wakame.

[0116] [Method of sensory evaluation]

[0117] The method of sensory evaluation was to evaluate the obtained boiled salted wakame after being soaked in 10 times the amount of tap water for 5 minutes to make it edible. The evaluation was performed by 10 people using the evaluation criteria of Table 2 below, and the average of the evaluation scores was symbolized according to the symbolization criteria below. The results are shown in Table 3.

[0118] [Symbolization criteria]

[0119] ◎: Evaluation score of 3.5 or more

[0120] O: Evaluation score of 2.5 or more and less than 3.5

[0121] Δ: Evaluation score of 1.5 or more and less than 2.5

[0122] X: Evaluation score of less than 1.5

[0123] [Table 2]

[0124]

[0125] [Table 3]

[0126]

[0127] As shown by the results in Table 3, the total mass of wakame obtained from the main rope into which the wakame seed line A was incorporated (the total amount of wakame A-1 and A-2) was less than that of wakame B-1 obtained from the main rope into which the wakame seed line B was incorporated, but the average mass of wakame A-3 per 1 plant was greater than that of wakame B-2, the deviation was small, and the homogeneity was increased. In addition, the taste of wakame A-3 was improved compared to that of wakame B-2. It can be said that the quality of wakame obtained by the method of the present application is improved.

Claims

1. A wakame seed line for wakame cultivation, characterized by, A coated portion having a dry oil attached thereto and an uncoated portion having no dry oil attached thereto are provided on a linear substratum, wherein the dry oil contains an iron salt.

2. A method for manufacturing a seed line of Undaria pinnatifida, which is a method for manufacturing a seed line of Undaria pinnatifida for cultivation of Undaria pinnatifida, characterized by A coated portion having a dry oil attached thereto and an uncoated portion having no dry oil attached thereto are provided on a linear substratum, and the linear substratum is contacted with a solution containing Undaria pinnatifida spores, so that the Undaria pinnatifida spores are mainly attached to the uncoated portion, wherein the dry oil contains an iron salt.

3. A method for culturing Undaria pinnatifida, characterized by, An Undaria pinnatifida seed line having a coated portion having a dry oil attached thereto and an uncoated portion having no dry oil attached thereto is installed on a main rope in a roll-in manner, and Undaria pinnatifida is formed by growing fronds in seawater, and the grown Undaria pinnatifida is harvested from the main rope, wherein the dry oil contains an iron salt.

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