Experimental Device for Fish Behavior Research and Its Usage Method

Abstract

The present invention relates to the technical field of experimental devices, and in particular provides an experimental device for fish behavior research and a usage method. The device includes a water storage pool, the output end of the water storage pool is connected to a sealed pressure device through a first water pump, and the output end of the sealed pressure device is connected to the input end of a water tank through a first valve; the output end of the water tank is connected to a test device through a second valve; the output end of the test device is connected to the water storage pool through a third valve; the device further includes: a temperature control device, arranged in the water storage pool, for keeping the water temperature in the water storage pool constant; a stirring device, installed in the water storage pool, for stirring the water in the water storage pool; a data acquisition device, for acquiring the water quality parameters of the water body in the water tank. The purpose is to achieve the research on fish behavior under the stress of various environmental factors alone or in combination, make the research on fish behavior more in line with reality, and provide necessary experimental data support for fishway design and reservoir operation.

Description

Technical Field

[0001] The present invention relates to the technical field of experimental devices, and more particularly, to an experimental device for fish behavior research and a using method thereof. Background Art

[0002] At present, with the large-scale development of hydropower projects, the construction of high dams has blocked the migration channels of fish, changed the hydrological regime of the downstream river channel, and seriously affected the migration, growth and reproduction of fish. Therefore, research on fish behavior, such as burst swimming speed, provides important basic data for fishway construction, fish avoiding enemies, etc.

[0003] Especially in recent years, with the large-scale emergence of cascade high dams, the total dissolved gas (TDG) supersaturation phenomenon appears in the downstream water body of the dam during flood discharge. The supersaturated water body with a high saturation of TDG can cause fish to suffer from bubble disease and even death, thus threatening the survival of fish; the construction of high dams has caused obvious water temperature stratification in the reservoir water body in front of the dam, and the water temperature of the discharged water body changes greatly; at the same time, the discharged water body often contains a large amount of sediment, which will also affect the survival of downstream aquatic organisms. On the other hand, at present, microplastics have become a new environmental pollution factor in water bodies. A relatively high concentration of microplastics has been detected in the water bodies of the Yangtze River Basin. When the supersaturation of TDG in the water body acts together with the above environmental factors such as sediment, microplastics, and temperature, it may exacerbate fish death.

[0004] At the present stage, the research on fish behavior mainly uses a single environmental factor as a stress factor, usually by exposing fish in a clear water environment; for example, in the prior art research on the impact of TDG supersaturation on fish swimming ability, the experimental fish are exposed to the TDG supersaturated water body and then transferred to a clear water environment to monitor the swimming ability of the fish. In actual engineering, fish may be continuously exposed to TDG supersaturation all the time. Therefore, there are certain limitations in the application of the above research results; there is a lack of experimental devices for the combined action of TDG supersaturation and other environmental factors, etc. For this reason, we propose an experimental device for fish behavior research and a using method thereof. Summary of the Invention

[0005] The purpose of the present invention is to provide an experimental device for fish behavior research and a using method thereof, so as to realize the research on fish behavior under the stress of various environmental factors alone or jointly, make the research on fish behavior more in line with actual needs, and provide necessary test data support for fishway design and reservoir operation.

[0006] The first aspect of the technical solution of the present invention provides an experimental device for fish behavior research, including a water storage pool. The output end of the water storage pool is connected to a sealed pressure device through a first water pump, and the output end of the sealed pressure device is connected to the input end of a water tank through a first valve; the output end of the water tank is connected to a test device through a second valve; the output end of the test device is connected to the water storage pool through a third valve; the device further includes: a temperature control device, arranged in the water storage pool, for keeping the water temperature in the water storage pool constant; a stirring device, installed in the water storage pool, for stirring the water in the water storage pool; a data acquisition device, for acquiring the water quality parameters of the water body in the water tank.

[0007] Further, the test device includes an annular fishway, and the annular fishway includes:

[0008] An inlet, arranged on one side of the annular fishway and connected to the output end of the water tank;

[0009] An outlet, arranged on one side of the inlet of the annular fishway and connected to the water storage pool;

[0010] A driving device, arranged at the inlet, for driving the water body to flow;

[0011] A flow guiding device, arranged between the inlet and the outlet, for maintaining the water flow velocity stable and uniform.

[0012] Further, the flow guiding device includes:

[0013] At least two flow guiding plates, and the space between the two flow guiding plates serves as the experimental section of the test device. A number of through holes are provided on the flow guiding plates.

[0014] Further, the sealed pressure device includes:

[0015] An air compressor, for conveying air into the sealed pressure device;

[0016] A pressure gauge, for detecting the pressure in the sealed pressure device.

[0017] Further, the experimental device further includes:

[0018] A fourth valve, whose input end is connected to the output end of the sealed pressure device, and the output end is connected to the water storage pool;

[0019] A second water pump, whose input end is connected to the water storage pool, and the output end is connected to the water tank through a fifth valve;

[0020] A sixth valve, whose input end is connected to the output end of the water tank, and the output end is connected to the water storage pool;

[0021] A seventh valve, whose input end is connected to the output end of the first water pump, and the output end is connected to the sealed pressure device;

[0022] The eighth valve, whose input end is connected to the output end of the first water pump and whose output end is connected to the stirring device.

[0023] Furthermore, the data collected by the data collection device at least includes the TDG saturation value, temperature value, turbidity value, and microplastic content, and the data collection device can collect one or more types of data.

[0024] The second aspect of the technical solution of the present invention provides a usage method of an experimental device for fish behavior research, which is applied to the experimental device provided by any one of the technical solutions in the first aspect of the technical solution of the present invention, and includes:

[0025] Add water into the water storage tank, and input air and water into the sealed pressure device simultaneously to form a TDG supersaturated water body;

[0026] Input the TDG supersaturated water body into the water tank and the water storage tank respectively;

[0027] Adjust the water temperature in the water storage tank to be constant at the first preset value through the temperature control device;

[0028] Use the stirring device to stir the water body in the water storage tank so that the water body in the water storage tank is in a TDG unsaturated state;

[0029] Inject the TDG unsaturated water body in the water storage tank into the water tank to form a TDG supersaturated water body with a preset saturation value and input it into the test device;

[0030] Control the water flow rate in the test device to the second preset value;

[0031] Gradually increase the water flow rate according to the preset time interval and the preset water flow rate increment value until the water flow rate reaches the third preset value.

[0032] Furthermore, forming a TDG supersaturated water body with a preset saturation value in the water tank specifically includes: using the second water pump to transport the water body in the water storage tank to the water tank, and detecting the TDG saturation value through the data collection device until the water body in the water tank reaches the preset TDG saturation value.

[0033] Furthermore, the usage method further includes: turning off the air compressor, making the water body in the experimental device be in a TDG unsaturated state through the stirring device, and then adding sediment and / or microplastics into the water storage tank.

[0034] Furthermore, the preset water flow rate increment value is the same as the second preset value.

[0035] The usage method of the experimental device for fish behavior research provided by the second aspect of the present invention is applied to the experimental device for fish behavior research described in any one of the technical solutions of the first aspect of the present invention. Therefore, it has all the beneficial effects of the experimental device for fish behavior research described in the technical solutions of the first aspect of this application, which will not be elaborated here.

[0036] The beneficial effects of the present invention include:

[0037] 1. The experimental device provided by the present invention has strong functionality and a wide range of applications. Through the combined use of a water storage tank, a sealed pressure device, a water tank, a temperature control device, a stirring device, and a testing device, it realizes the research on the effects of environmental factors such as TDG supersaturation, sediment, microplastics, and temperature on fish behavior alone or in combination, providing necessary experimental equipment support for research on the effects of environmental factors such as gas supersaturation, sediment, and microplastics caused by dam sluicing on fish, fishway design, reservoir operation, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required to be used in the embodiments of the present invention. It should be understood that the following drawings only show some embodiments of the present invention, and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0039] Figure 1 It is a schematic diagram of the overall structure of the experimental device for fish behavior research provided by the embodiment of the present invention;

[0040] Figure 2 It is a schematic diagram of the structure of the water storage tank provided by the embodiment of the present invention;

[0041] Figure 3 It is a schematic diagram of the structure of the testing device provided by the embodiment of the present invention;

[0042] Figure 4 It is a schematic diagram of the steps of the usage method of the experimental device for fish behavior research provided by the embodiment of the present invention;

[0043] Figure 5 It is a bar chart showing the relationship between the burst swimming speed and the TDG saturation and sediment concentration provided by the present invention;

[0044] Figure 6 It is a linear relationship diagram between the TDG saturation and the burst swimming speed under different sediment concentrations provided by the present invention;

[0045] Figure 7 It is a linear relationship diagram between the sediment concentration and the burst swimming speed under different TDG saturations provided by the present invention;

[0046] Icons: 100 - water storage tank, 110 - first water pump, 111 - seventh valve, 120 - second water pump, 200 - sealed pressure device, 210 - first valve, 220 - air compressor, 230 - pressure gauge, 240 - fourth valve, 300 - water tank, 310 - second valve, 320 - fifth valve, 330 - sixth valve, 400 - test device, 410 - third valve, 420 - annular fishway, 421 - water inlet, 422 - water outlet, 423 - driving device, 424 - guide vane, 425 - experimental section, 500 - temperature control device, 600 - stirring device, 610 - eighth valve. Specific embodiments

[0047] The technical solutions in the embodiments of the present invention will be described below with reference to the accompanying drawings in the embodiments of the present invention.

[0048] It should be noted that: similar reference numerals and letters denote similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings. At the same time, in the description of the present invention, the terms "first", "second", etc. are only used for distinguishing descriptions and cannot be understood as indicating or implying relative importance.

[0049] Please refer to Figure 1 As shown, the first aspect of the technical solution of the present invention provides an experimental device for fish behavior research, including:

[0050] A water storage tank 100, the output end of the water storage tank 100 is connected to the sealed pressure device 200 through the first water pump 110; before the experiment, the water storage tank 100 should be filled with the amount of water required for the experiment;

[0051] The output end of the sealed pressure device 200 is connected to the input end of the water tank 300 through the first valve 210; the output end of the sealed pressure device 200 is also connected to the water storage tank 100 through the fourth valve 240, and its purpose is to control the amount of water in the sealed pressure device 200 during use and discharge the excess water in the sealed pressure device 200 into the water storage tank 100; the sealed pressure device 200 includes: an air compressor 220 for delivering air into the sealed pressure device 200; a pressure gauge 230 for detecting the pressure in the sealed pressure device 200; the main function of the sealed pressure device 200 is to form a TDG supersaturated water body in a sealed and ventilated environment. In actual application, the water in the water storage tank is injected into the sealed pressure device 200 through the first water pump 110, and at the same time, the compressed air is also injected into the sealed pressure device 200 through the air compressor 220, so that the water body in the sealed pressure device 200 is in a supersaturated state to form the water body required for the experiment;

[0052] The output end of the water tank 300 is connected to the testing device 400 through the second valve 310; the output end of the water tank 300 is also connected to the storage pool 100 through the sixth valve 330, and its purpose is to control the water volume in the water tank 300 during use and drain the excess water in the water tank 300 into the storage pool 100;

[0053] Please refer to Figure 3 As shown, the output end of the testing device 400 is connected to the storage pool 100 through the third valve 410; the testing device 400 includes an annular fishway 420, and the annular fishway 420 includes: a water inlet 421, which is arranged on one side of the annular fishway 420 and is connected to the output end of the water tank 300; a water outlet 422, which is arranged on one side of the water inlet 421 of the annular fishway 420 and is connected to the storage pool 100; a driving device 423, which is arranged at the water inlet 421 and is used to drive the water body to flow;

[0054] Among them, the driving device 423 is preferably configured to drive the propeller to rotate through a variable-frequency motor to drive the water body to accelerate the flow, and control the rotation speed of the propeller through the change of the frequency of the variable-frequency motor to control the flow rate of the water body on the annular fishway 420. The driving device 423 can also adopt a water body acceleration device in the prior art, such as the water body acceleration device in the self-circulating high-density suspended sludge filtration and sedimentation device with the publication number CN215479956U, as long as it can realize driving the water body to accelerate the flow on the annular fishway 420;

[0055] A flow guiding device is arranged between the water inlet 421 and the water outlet 422 and is used to maintain the stable and uniform flow rate of the water flow. The flow guiding device includes: at least two flow guiding plates 424, and the space between the two flow guiding plates 424 serves as the experimental section 425 of the testing device 400. A number of through holes are provided on the flow guiding plate 424, and the diameter of the through holes should be configured to be a size that cannot allow the experimental fish to pass through, so as to ensure that the experimental fish is always in the experimental section 425 during the experiment. The size of the through holes is not limited here;

[0056] The following is a further description of the testing device 400:

[0057] As Figure 3As shown, before studying fish behavior using the testing device 400, the water in the water tank 300 is put into the annular fishway 420 through the water inlet 421 to fill the annular fishway 420 with water. Subsequently, the propeller is rotated by a variable-frequency motor to drive the water flow on the annular runway. Two flow guiding plates 424 are arranged at intervals on the annular fishway 420. The area (experimental section) between the two flow guiding plates 424 can be understood as the experimental area on the annular fishway 420. It can be understood that after the water is accelerated, it passes through the first straight section, the first bend, the second straight section, and the second bend in sequence and circulates in this way. One flow guiding plate 424 is arranged at the outlet of the first bend, and the other flow guiding plate 424 is arranged at the inlet of the second bend. The flow guiding plates 424 are used to make the water flow in the experimental area stable and uniform, avoiding the influence of turbulent and unstable water flow on the experimental process.

[0058] The temperature control device 500 is arranged in the storage pool 100 and is used to keep the water temperature in the storage pool 100 constant. By keeping the water temperature at different temperatures, various experimental conditions can be met. The temperature control device 500 in the present invention is based on being able to keep the water temperature in the storage pool 100 constant. For example, the constant-temperature water tank 300 in the invention patent with the publication number CN110260516B, the water heater, and the constant-temperature water tank 300 in the water temperature control method of the constant-temperature water tank 300 can be used. For example, the invention patent cold and hot water mixing constant-temperature controller with the publication number CN103498949B. The temperature control device 500 is not limited here.

[0059] The stirring device 600 is installed in the storage pool 100 and is used to stir the water in the storage pool 100. An eighth valve 610 is also provided at the input end of the stirring device 600. The purpose is that if environmental factors such as sediment and microplastics need to be added to the storage pool 100, the water is directly input into the storage pool 100 through the eighth valve 610 and stirred by the stirring device 600 to keep the environmental factors such as sediment and microplastics evenly distributed. On the other hand, the stirring device 600 can also be used to accelerate the dissipation of dissolved gas in the storage pool 100 and keep the water in the storage pool 100 in a supersaturated state to adjust the saturation value in the water tank 300. As Figure 2 shown, the stirring device 600 is arranged in the storage pool 100 and includes a main input pipeline and several U-shaped pipelines that can swing left and right. The water flows out through the main input pipe and is discharged into the storage pool 100 through several U-shaped pipelines. The purpose is to uniformly stir the water body in the storage pool 100 and make the distribution of environmental factors in the water body in the storage pool as uniform as possible. The driving mode of the stirring device 600 can be selected in a manual and / or automatic way. If manual driving is adopted, a handle that can control the swing of the stirring device 600 can be added to the storage pool 100. If electric driving is adopted, an existing motor can be used and will not be elaborated here.

[0060] A data acquisition device is used to acquire water quality parameters of the water body in the water tank 300. The data acquired by the data acquisition device at least includes TDG saturation value, temperature value, turbidity value, and microplastic content. The data acquisition device can acquire one or more types of data. The data acquisition device can use instruments in the prior art, such as Point Four Tracker total air pressure detector, YSI6600V2 multi-parameter water quality detector, etc., to realize the acquisition of water quality parameters.

[0061] In summary, the experimental device for studying fish behavior provided by the present invention has a simple structure, strong functionality, and a wide range of applications. Through the combined use of a storage pool, a sealed pressure device, a water tank, a temperature control device, a stirring device, and a testing device, it realizes the research on the effects of environmental factors such as TDG supersaturation, sediment, microplastics, and temperature acting alone or in combination on fish behavior. It provides necessary experimental equipment support for studying the effects of environmental factors such as gas supersaturation, sediment, and microplastics caused by dam sluicing, etc. on fish, as well as in fishway design, reservoir operation, etc.

[0062] Swimming is an important physiological activity of fish and is the main way to realize behaviors such as foraging, escaping from enemies, upstream migration, and reproduction of fish, which is of great significance to the growth, development, and survival of fish. Among them, the burst swimming speed (Uburst) refers to the maximum swimming speed that fish can reach in a short time and is one of the important indicators to evaluate fish swimming ability. In some special structures and high-speed water flow areas of fishways, fish usually rely on burst swimming speed to pass through. The following of the present invention takes the study of the effects of TDG supersaturated water containing sediment on fish behavior as an example. It should be noted that if it is necessary to carry out the study on the effects of environmental factors such as microplastics and temperature acting alone on fish behavior, only the air compressor 220 in the above embodiments needs to be turned off; if it is necessary to carry out the study on the effects of the combined action of TDG supersaturation and the above environmental factors on fish behavior, the environmental factors to be studied can be added to the storage pool 100, and its usage method is the same as the principle of studying the effects of TDG supersaturated water on fish.

[0063] As Figure 4 shown, the second aspect of the technical solution of the present invention provides a usage method of an experimental device for studying fish behavior, which is applied to the experimental device provided by any one of the technical solutions in the first aspect of the technical solution of the present invention, including:

[0064] Step S1: Add water to the storage pool 100, turn on the first water pump 110 and the air compressor 220, and input air and water into the sealed pressure device 200 at the same time to form TDG supersaturated water.

[0065] Step S2: Input the TDG supersaturated water body into the water tank 300 and the storage pool 100 respectively;

[0066] Specifically, it includes: inputting the TDG supersaturated water body into the water tank 300, and according to the experimental needs, the excess TDG supersaturated water body is discharged into the storage pool 100 through the fourth valve 240;

[0067] Step S3: Adjust the water temperature in the storage pool 100 to a first preset value through the temperature control device 500;

[0068] Among them, step S3 specifically includes that the first preset value is defined according to the actual experimental requirements. The temperature in the storage pool 100 is adjusted through the temperature control device 500 to study the influence of temperature as a single environmental factor or in combination with other environmental factors on fish behavior. For example, when studying the combined effect of three environmental factors of temperature, sediment concentration, and TDG supersaturation on fish behavior, the temperature control device 500 and the stirring device 600 need to be used simultaneously; if temperature is not used as an environmental factor for research, only the water temperature in the storage pool 100 needs to be kept constant at a water temperature suitable for fish survival through the temperature control device 500;

[0069] Step S4: Use the stirring device 600 to stir the water body in the storage pool 100 so that the water body in the storage pool 100 is in a TDG unsaturated state;

[0070] Step S5: Inject the TDG unsaturated water body in the storage pool 100 into the water tank 300 to form a TDG supersaturated water body with a preset saturation value and input it into the test device 400;

[0071] Specifically, it includes: using the second water pump 120 to transport the water body in the storage pool 100 to the water tank 300, and detecting the TDG saturation value through the data acquisition device until the water body in the water tank 300 reaches the preset TDG saturation value.

[0072] Step S6: Control the water body flow rate in the test device 400 to a second preset value;

[0073] Step S7: Gradually increase the water body flow rate according to the preset time interval and the preset water body flow rate increment value until the water body flow rate reaches a third preset value.

[0074] Furthermore, this usage method further includes: turning off the air compressor 220, using the stirring device 600 to make the water body in the experimental device in a TDG unsaturated state, and then adding sediment and / or microplastics into the storage pool 100.

[0075] Furthermore, the preset water body flow rate increment value is the same as the second preset value.

[0076] Taking the impact of TDG supersaturated sediment-laden water with different sediment concentrations and TDG saturations on the burst swimming speed of Procypris rabaudi as an example, the beneficial effects brought by the experimental device and usage method for fish behavior research will be described in detail as follows:

[0077] In this embodiment, the specific configurations of the relevant parameters of the test device are as follows: the size of the storage pool 100 is 300 cm * 300 cm * 200 cm (length * width * height); the size of the experimental section 425 between the guide plates of the test device 400 is 60 cm * 20 cm * 20 cm (length * width * height); the size of the guide plate 424 is 20 mm * 20 mm (length * width); the size of the through holes on the guide plate 424 is 3 mm * 3 mm (length * width). Among them, for the sizes of the storage pool 100, the experimental section 425, the guide plate 424, and the through holes on the guide plate 424, in actual operation, the relevant sizes can be adjusted according to actual experimental requirements to meet the experimental needs. This embodiment does not limit their sizes.

[0078] First, connect the relevant equipment and instruments according to the above experimental device layout. Select sediment with a median particle size of 7 u μm as the test sand. As shown in Table 1, set 5 TDG saturation groups of 100%, 125%, 130%, 135%, and 140%, and 4 sediment concentration groups of 0 mg / L, 50 mg / L, 100 mg / L, and 150 mg / L. There are a total of 20 experimental conditions by combining them in pairs. Specifically, when using this experimental device, through the above step S5 (forming the above 5 TDG saturations) and the agitation device 600 (forming the water bodies with the above 4 sediment concentrations), through repeated adjustment and multiple experiments, the experimental conditions under the combination of different TDG saturations and different sediment concentrations in pairs are realized.

[0079] Table 1 Experimental situation table of working conditions

[0080] Operating condition Sediment concentration + TDG saturation Operating condition Sediment concentration + TDG saturation Operating condition 1 0 mg / L + 100% TDG Operating condition 11 100 mg / L + 100% TDG Operating condition 2 0 mg / L + 125% TDG Operating condition 12 100 mg / L + 125% TDG Operating condition 3 0 mg / L + 130% TDG Operating condition 13 100 mg / L + 130% TDG Operating condition 4 0 mg / L + 135% TDG Operating condition 14 100 mg / L + 135% TDG Operating condition 5 0 mg / L + 140% TDG Operating condition 15 100 mg / L + 140% TDG Operating condition 6 50 mg / L + 100% TDG Operating condition 16 150 mg / L + 100% TDG Operating condition 7 50 mg / L + 125% TDG Operating condition 17 150 mg / L + 125% TDG Operating condition 8 50 mg / L + 130% TDG Operating condition 18 150 mg / L + 130% TDG Operating condition 9 50 mg / L + 135% TDG Operating condition 19 150 mg / L + 135% TDG Operating condition 10 50 mg / L + 140% TDG Operating condition 20 150 mg / L + 140% TDG

[0081] Table 1 records the 20 experimental conditions after combining the above 5 TDG saturations and 4 sediment concentrations in pairs.

[0082] Then, make the water in the annular fishway 420 flow through the test device 400, place the experimental fish in the experimental area (i.e., the above experimental section 425). As shown in Table 2, record the relevant data of the experimental fish in the above experimental area, and calculate the burst swimming speed of the experimental fish according to the following calculation formula:

[0083] U burst =U max +(t / ΔT)×ΔU;

[0084] Among them, U burst$V_{max}$ is the maximum swimming speed (BL / s) that the experimental fish can reach after completing the duration ($\Delta T$). BL represents the body length of the experimental fish, and $t$ is the swimming time (min) of the experimental fish at the highest flow rate ($t \lt \Delta T$); $\Delta T$ is the time interval (1 min) for the flow rate to increase; $\Delta U$ is the speed increment (1 BL / s) when the flow rate increases;

[0085] Table 2 Record table of the burst swimming speed of experimental fish under each working condition

[0086] Operating condition Sediment concentration + TDG saturation <![CDATA[U burst (BL / s)]]> Operating condition Sediment concentration + TDG saturation <![CDATA[U burst (BL / s)]]> Operating condition 1 0 mg / L + 100% 7.36 Operating condition 11 100 mg / L + 100% 7.05 Operating condition 2 0 mg / L + 125% 5.4 Operating condition 12 100 mg / L + 125% 5.3 Operating condition 3 0 mg / L + 130% 5.12 Operating condition 13 100 mg / L + 130% 4.76 Operating condition 4 0 mg / L + 135% 4.72 Operating condition 14 100 mg / L + 135% 4.53 Operating condition 5 0 mg / L + 140% 4.51 Operating condition 15 100 mg / L + 140% 4.49 Operating condition 6 50 mg / L + 100% 7.13 Operating condition 16 150 mg / L + 100% 7.04 Operating condition 7 50 mg / L + 125% 5.34 Operating condition 17 150 mg / L + 125% 5.23 Operating condition 8 50 mg / L + 130% 5.08 Operating condition 18 150 mg / L + 130% 4.6 Operating condition 9 50 mg / L + 135% 4.57 Operating condition 19 150 mg / L + 135% 4.4 Operating condition 10 50 mg / L + 140% 4.5 Operating condition 20 150 mg / L + 140% 4.23

[0087] Table 2 is the record table of the burst swimming speed corresponding to an experimental fish under 20 working conditions;

[0088] Furthermore, for each experimental working condition, 10 experimental fish were selected to repeat the above experimental process to obtain the burst swimming speed of each experimental fish, and finally the average value was taken as the burst swimming speed of the final experimental fish;

[0089] Please refer to Figure 5 as shown. It can be understood that when TDG is supersaturated and sediment acts alone, the burst swimming speed of the experimental fish decreases with the increase of saturation and sediment concentration respectively. Compared with the situation where TDG is supersaturated and sediment acts alone, after the experimental fish is affected by the combined action of the two, the burst swimming speed decreases, that is, the combined action of TDG supersaturation and sediment has a greater impact on the burst swimming speed of fish than the individual action of the two;

[0090] Please refer to Figure 6 and Figure 7 as shown. It can be understood that under different sediment concentration conditions, there is an obvious negative correlation between TDG saturation and the burst swimming speed of the experimental fish; under different TDG saturation conditions, there is also an obvious negative correlation between sediment concentration and the burst swimming speed of the experimental fish; at the same TDG saturation (or sediment concentration), the burst swimming speed of the experimental fish decreases with the increase of sediment concentration (or TDG saturation);

[0091] In summary, it can be understood that through the experimental device, the effects of different TDG supersaturations, different concentrations of sediment acting alone or in combination on the burst swimming speed of Procypris rabaudi can be studied;

[0092] In this embodiment, Procypris rabaudi is taken as an example for illustration. The device and its usage method can also be applicable to the research of freshwater fish such as sturgeon and crucian carp, and only the size parameters of relevant equipment in the experimental device need to be changed. It can also be understood that in this embodiment, the TDG supersaturated water body with TDG supersaturation and sediment as interference factors is used for illustration. The device and its usage method can also be applicable to the research of fish behavior under interference factors such as microplastics and temperature. It can also be understood that in this embodiment, the burst swimming speed for evaluating the swimming ability of fish is taken as an example for illustration. The device and its usage method can also be applicable to the research of aspects such as the critical swimming speed, induced swimming speed, and fish tolerance of fish, which will not be elaborated here.

[0093] Thus, it can be seen that the fish behavior research device and its usage method provided by the present invention can realize the research of fish behavior under the stress of various environmental factors alone or in combination, making the research on fish behavior more in line with reality and providing important references for the proposal of solutions such as fishway construction and reservoir operation.

[0094] The usage method of the fish behavior research experimental device provided by the second aspect technical solution of the present invention is applied to the fish behavior research experimental device described in any item of the first aspect technical solution of the present invention. Therefore, it has all the beneficial effects of the fish behavior research experimental device described in the first aspect technical solution of this application, which will not be elaborated here.

[0095] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

1. Experimental device for fish behavior research, characterized in that, it includes a water storage tank, the output end of the water storage tank is connected to a sealing pressure device through a first water pump, and the output end of the sealing pressure device is connected to the input end of a water tank through a first valve; the output end of the water tank is connected to a testing device through a second valve; the output end of the testing device is connected to the water storage tank through a third valve; the testing device includes an annular fishway, and the annular fishway includes: a water inlet, arranged on one side of the annular fishway and connected to the output end of the water tank; a water outlet, arranged on one side of the water inlet of the annular fishway and connected to the water storage tank; a driving device, arranged at the water inlet, for driving the water body to flow; a flow guiding device, arranged between the water inlet and the water outlet, for maintaining the stable and uniform flow velocity of the water flow; the flow guiding device includes: at least two flow guiding plates, and the space between the two flow guiding plates serves as the experimental section of the testing device, and a plurality of through holes are provided on the flow guiding plates; the sealing pressure device includes: an air compressor, for conveying air into the sealing pressure device; a pressure gauge, for detecting the pressure inside the sealing pressure device; the device further includes: a temperature control device, arranged in the water storage tank, for keeping the water temperature in the water storage tank constant; a stirring device, installed in the water storage tank, for stirring the water in the water storage tank; a data acquisition device, for acquiring the water quality parameters of the water body in the water tank; a fourth valve, its input end is connected to the output end of the sealing pressure device, and its output end is connected to the water storage tank; a second water pump, its input end is connected to the water storage tank, and its output end is connected to the water tank through a fifth valve; a sixth valve, its input end is connected to the output end of the water tank, and its output end is connected to the water storage tank; a seventh valve, its input end is connected to the output end of the first water pump, and its output end is connected to the sealing pressure device; an eighth valve, its input end is connected to the output end of the first water pump, and its output end is connected to the stirring device.

2. The experimental device according to claim 1, characterized in that, the data collected by the data acquisition device at least includes TDG saturation value, temperature value, turbidity value, microplastic content, and the data acquisition device can collect one or more kinds of data.

3. A method for using an experimental device for fish behavior research, applied to the experimental device according to any one of claims 1 to 2, characterized in that, it includes: adding water into the water storage tank, and simultaneously inputting air and water into the sealing pressure device to form a TDG supersaturated water body; inputting the TDG supersaturated water body into the water tank and the water storage tank respectively; adjusting the water temperature in the water storage tank to a first preset value through the temperature control device; using the stirring device to stir the water body in the water storage tank so that the water body in the water storage tank is in a TDG unsaturated state; injecting the TDG unsaturated water body in the water storage tank into the water tank to form a TDG supersaturated water body with a preset saturation value and input it into the testing device; controlling the water flow velocity in the testing device to a second preset value; gradually increasing the water flow velocity according to a preset time interval and a preset increment value of the water flow velocity until the water flow velocity reaches a third preset value.

4. The use method according to claim 3, characterized in that, Forming a TDG supersaturated water body with a preset saturation value in the water tank specifically includes: using a second water pump to transport the water body in the reservoir to the water tank, and detecting the TDG saturation value through a data acquisition device until the water body in the water tank reaches the preset TDG saturation value.

5. The usage method according to claim 3, characterized in that, the usage method further includes: closing the air compressor, making the water bodies in the experimental device in a TDG unsaturated state through a stirring device, and then adding sediment and / or microplastics into the reservoir.

6. The usage method according to claim 3, characterized in that, the preset water flow rate increasing increment value is consistent with the second preset value.

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