A multi-station assembly line for immunohistochemistry and HE atomization staining and sealing

Abstract

The present invention discloses a multi-station assembly line for immunohistochemistry and HE atomization staining and sealing, comprising a housing, an incubation module, a cover template cleaning module, a mobile liquid adding module, a tray, a staining module, a centrifugal liquid throwing module, a glue sealing module and a high-temperature repair water bath module are arranged in the housing, the staining module and the glue sealing module are both located above the tray, the centrifugal liquid throwing module is located below the tray, and can drive the tray to rotate horizontally, an incubation module and a high-temperature repair water bath module are arranged outside the staining module, the tray can be moved to the incubation module and the high-temperature repair water bath module under the drive of a sliding conveying mechanism, and the staining module is isolated from the incubation module and the high-temperature repair water bath module by a movable isolation component, a cover template cleaning module that can move up and down is provided below the incubation module, and a mobile liquid adding module is provided above the incubation module. The equipment has a high degree of automation, good processing effect, high work efficiency, and saves a lot of labor costs.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a multi-station assembly line for immunohistochemistry and HE atomization staining and sealing. Background Art

[0002] There are three main types of pathological cell immunohistochemistry and in situ hybridization staining equipment at home and abroad, which are imported, including Leica immunohistochemistry stainer, Agilent immunohistochemistry stainer and Roche immunohistochemistry stainer; there are two main types of pathological cell HE staining equipment, including immersion HE staining equipment and drop HE staining equipment.

[0003] Both Leica and Agilent immunohistochemical stainers place a plastic cover over the sample slide for incubation. Staining reagent is dripped in from the tilted cover, which is higher than the sample slide, and excess is removed from the lower side. A small gap is left between the plastic cover and the slide, allowing the dripping reagent to form a siphon effect. A robotic arm is used to move the sample needle for sample addition, and a heating and temperature control device is located under the sample slide. Most domestically produced machines are similar to the Leica immunohistochemical stainer. Roche immunohistochemical stainers drip reagent directly onto the sample slide, mixing it with air through a nozzle. A liquid cover film reagent replaces the plastic cover film, and after incubation, the liquid cover film is rinsed off with a cleaning solution. A heating and temperature control device is located under the sample slide. In HE stainers, immersion staining uses a robotic arm to replace traditional manual labor, immersing sample slides placed in a hanging basket into multiple staining vats for staining. Drip staining uses a pump to pump reagent onto the sample slide, and a nozzle to remove the waste liquid.

[0004] The above-mentioned immunohistochemical staining machine has the following defects when in use: (1) The number of slides on the machine is small and the speed is slow (generally only 30 slides can be processed at a time, and it takes several hours to complete); (2) high temperature and low temperature, contaminated area and clean area, open area and closed area are all in the same workstation area. High temperature repair is carried out in a small plastic cover at nearly 100°C, and the repair liquid is only 50 to 100 microliters. This repair effect is poor and it is easy to dry the slides. The temperature of different steps changes repeatedly from room temperature 25°C to 100°C, and the temperature is difficult to control; DAB staining solution has serious corrosive pollution, and hematoxylin staining solution is also contaminated. Antibody reagents need to be carried out in a clean area. Immunohistochemical staining machines such as Leica and Agilent only operate in one workstation in a closed plastic cover. The contaminated area and the clean area are mixed together, causing cross contamination and background interference; cleaning, dewaxing and other operations need to be done in an open space for cleaning and dewaxing to be thorough, so the steps that should be completed in multiple workstations are The process is completed in a station under a sealed plastic cover, which makes the cleaning and dewaxing not clean, and interferes with each other and cannot be taken into account, which greatly affects the staining quality. The liquid cover film technology in the Roche immunohistochemical staining machine solves the problem that only the antibody is enclosed in the liquid film during incubation, and the sample slides are open in other steps. However, the liquid cover film needs to be repeatedly rinsed before it is removed, which makes it easy for the slides to fall off. The equipment also has the problem of only one station. The method of mixing the reagents on a single sample slide by blowing air through the nozzle is slow and inefficient. Therefore, hospitals generally report that the Roche immunohistochemical staining machine is easy to fall off when used, and the Leica immunohistochemical staining machine has background interference when used; (3) It is impossible to combine immunohistochemistry and HE staining into one machine, and it cannot be multi-purpose or modularized for low-cost production; (4) The staining reagent is easily condensed into liquid beads when dropped on the smooth slide and cannot be evenly dispersed; (5) The loading and unloading process of the sample slides requires several manual transfers, which is labor-intensive.

[0005] The aforementioned HE staining machine immersion dyeing method has the following drawbacks: The immersion dyeing equipment (hanging basket + dye vat) has the following drawbacks: Because the dye vat containing the reagent is open, the reagent evaporates and the concentration slowly decreases. Furthermore, if the dye vat is left in the dye vat for a while, tissue sample fragments will fall off, resulting in reduced dyeing quality, increased cross-contamination, decreased yield, and reduced stability in later dyeing batches. When the reagent concentration decreases, technicians are completely required to manually add a small amount of new reagent, which is highly uncertain. Furthermore, the reagent update relies entirely on technicians operating in a contaminated environment, which is harmful to their health. The immersion dyeing sealant: After the slides are stained, the hanging basket is removed from the dye vat by a robot and transferred to the sealant. During the sealant process, the slides must be removed from the hanging basket one by one, sealed with glue, and finally placed on a slide reading board. The loading and unloading process of the sample slides requires several manual transfers, which is labor-intensive. Furthermore, because the sealant is a single sheet, it is impossible to achieve the optimal wet seal state on the sample slide before the xylene dries, seriously affecting the quality of the slide. HE dyeing machine drip dyeing equipment: Drip dyeing is a new generation of technological innovation that can avoid the shortcomings of immersion dyeing. However, the Roche drip dyeing method currently on the market has the following problems: high reagent consumption, large amount of waste liquid, and time-consuming. Because the surface of the glass slide is smooth, the dye will slide away when dripped on it. At the same time, the droplets are cohesive, forming large liquid beads or several small liquid beads, resulting in uneven dyeing. To solve this problem, the drip dyeing method adopts the method of dripping a large amount of reagent, placing the glass slide in a clamp to allow the reagent to soak the surface of the glass slide, so the reagent consumption is high. At the same time, it is time-consuming because the liquid must be dripped after alignment of each slide. Both immersion dyeing and drip dyeing equipment have the problems of complex, difficult, and time-consuming cleaning. Immersion dyeing equipment uses multiple cylinders of cleaning liquid for cleaning, and the water is allowed to slowly flow down and dry when cooling the slide. Drip dyeing equipment uses slides one by one to absorb the waste liquid. In addition, the equipment has a complex mechanical structure, a high failure rate, is time-consuming, and the equipment is expensive. Summary of the Invention

[0006] In order to solve the problems existing in the above-mentioned background technology, the present invention provides a multi-station production line for immunohistochemistry and HE atomization staining and sealing, which has a convenient and fast loading and unloading process, can process sample slides in large quantities, reduces the phenomenon of falling and drying slices during use, reduces background interference, realizes real wet sealing, and achieves the best production effect. Atomization staining and centrifugal liquid removal are realized, the high temperature zone and the low temperature zone are separated to form an independent closed working area, and the contaminated area and the clean area are separated to form an independent closed working area without affecting each other, and staining, dewaxing, cleaning and repair are more thorough. Staining, sealing and immunohistochemistry are all independent modules, which can be freely combined into different functional systems to realize multi-functional and standardized production. The equipment has a high degree of automation and high efficiency, saving a lot of labor costs.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] The present invention provides a multi-station production line for immunohistochemistry and HE atomization staining and sealing, comprising a housing, wherein an incubation module, a cover template cleaning module, a mobile liquid adding module, a tray, a staining module, a centrifugal liquid throwing module, a glue dripping and sealing module, and a high-temperature repair water bath module are arranged in the housing;

[0009] A plurality of reading plates are evenly inserted on the top of the tray, and the ends of the reading plates are fixed to the top of the tray. The top of the reading plates is evenly provided with rows of receiving grooves for mounting sample slides, and the ends of the sample slides are fixed in the receiving grooves. The tray and the reading plates are hollow structures. The cooperation of the reading plates and the tray facilitates loading and unloading, and 120-1200 sample slides can be loaded on the machine at a time. In addition, the tray and the reading plates are hollow structures, which facilitates the removal of waste liquid during staining.

[0010] The staining module and the glue sealing module are both located above the tray, and the centrifugal liquid-spinning module is located below the tray, and can drive the tray to rotate in the horizontal direction to centrifugally remove the staining reagent on the sample slide or to spin the liquid while spraying the cleaning liquid. The incubation module and the high-temperature repair water bath module are provided on the outside of the staining module. The tray can be moved to the incubation module and the high-temperature repair water bath module under the drive of the sliding conveying mechanism, and the staining module is isolated from the incubation module and the high-temperature repair water bath module by a movable isolation component;

[0011] The incubation module can accommodate the tray, and a cover template assembly and a driving device are provided on the top of the incubation module. The cover template assembly includes a fixed plate and multiple cover templates. The fixed plate is provided with multiple long slots for sample addition, and the sample addition long slots are alternately distributed corresponding to the multiple cover templates. The fixed plate can move along the Y-axis and Z-axis directions under the drive of the driving device, and the fixed plate is located above the tray, and multiple cover templates are installed at the bottom of the fixed plate. A cover template cleaning module that can move up and down is provided under the incubation module, and the fixed plate moves in the Y-axis direction under the drive of the driving device, so that each sample addition long slot on the fixed plate is directly opposite to the sample slides coated with cells below. The center of the area is then provided above the incubation module, and the mobile liquid adding module is used to add reagents to the sample glass slide on the tray through the mobile liquid adding module. Then the fixed plate is retracted and moved in the opposite direction of the Y axis under the drive of the driving device, so that the cover template is facing the center of the cell area on the sample glass slide, and then the cover template bottom plate is controlled to move along the Z axis, so that the cover template is gently pressed downward to seal the antibody reagent between the cover template and the sample glass slide. A cover template cleaning module is provided below the incubation module. After incubation, the cover template rises. After the tray is pulled away, the cover template is lowered into the cleaning liquid tank of the cover template cleaning module to immerse it and repeatedly move up and down to oscillate and clean the reagent on the cover template.

[0012] The cover plate comprises an upper cover plate and a lower cover plate. A heating plate is located in a cavity in the center of the lower cover plate. One of the two pillars on the upper cover plate has a through hole for routing wiring connected to the heating plate. The bottom surface of the lower cover plate is a smooth, flat surface with a liquid-blocking border, and an elastic buffer assembly is provided between the lower cover plate and the fixed plate. When the cover plate moves downward, it contacts the sample slide, generating an elastic contact force to prevent damage to the sample slide.

[0013] A further improvement is that the top of the tray is provided with baffles on both the front and rear sides, and a plurality of isolation plates arranged side by side with the baffles are provided on the top of the tray. The baffles and isolation plates divide the top of the tray into multiple rows of independent cavities for mounting reading boards. The top of the tray is provided with blocks and elastic stoppers at both ends of the independent cavities for limiting and fixing the ends of the reading boards, and the bottom of the tray is provided with pulleys. During use, the above structure can fix and limit the position of the reading boards placed on the tray.

[0014] A further improvement is that the two opposite sides of the reading board are respectively provided with a hanging buckle and a hook groove, and the two adjacent reading boards are fixed together by the hanging buckle and the hook groove. When in use, the hanging buckle of the front reading board is matched with the hook groove of the rear reading board, so that multiple reading boards can be connected to form a whole, which is convenient for installing it on the tray or taking it out from the tray. The connection between the four adjacent receiving grooves on the reading board is provided with an upper baffle for limiting the top of the sample glass slide in the receiving groove. The two ends of the reading board along the length direction are rotatably provided with a pressing plate, and the pressing plate is rotatably provided at both ends along the length direction. The ends are respectively provided with elastic handle heads, and the reading board is provided with handle slots which are engaged with the elastic handle heads. By engaging the elastic handle heads into the handle slots, the pressing plate can be pressed down to above the sample glass in the receiving slot. When in use, each sample glass is inserted into the receiving slot on the reading board until the end of the sample glass is inserted under the upper baffle. After all the sample glass slides are inserted, the pressing plate is rotated until the elastic handle head on the pressing plate is engaged with the handle slot. At this time, the pressing plate is pressed on the other end of the sample glass, so that the sample glass can be fixed in the receiving slot on the reading board.

[0015] A further improvement is that support foot fixing grooves are formed at the four corners of the top of the reading board, and support feet are provided at the four corners of the bottom of the reading board. Through this arrangement, multiple reading boards can be stacked together for transportation, making it convenient to move multiple reading boards.

[0016] A further improvement is that the dyeing module includes multiple atomizing head assemblies, an atomizing control box, an installation frame, a spray assembly mist shield and a driving mechanism, multiple atomizing head assemblies are installed side by side at the lower part of the installation frame, the atomizing control box is provided on the upper part of the installation frame, and the installation frame can move along the X-axis and Y-axis directions under the drive of the driving mechanism, and the spray assembly mist shield is correspondingly installed at the bottom end of each of the atomizing head assemblies, and the spray assembly mist shield can block the mist liquid in the area of ​​several sample slices currently spraying liquid to prevent the mist liquid from spreading and contaminating other components. The atomizing head assembly includes multiple liquid inlet pipes, a connecting body, a liquid inlet fixed adjusting column, an air nozzle threaded column, a tightening nut, a mist diffuser, and a mist crushing ball. Multiple liquid inlet pipes are arranged side by side on the connecting body, and the outer side of the upper part of the liquid inlet pipe is threadedly connected to the connecting body through the liquid inlet fixed adjusting column. A mist diffuser is provided at the position corresponding to the liquid inlet pipe at the bottom of the connecting body, and the upper part of the mist diffuser is threadedly connected with the air nozzle threaded column, and the upper part of the air nozzle threaded column is arranged on the connecting body The lower part of the liquid inlet pipe passes through the air nozzle thread column and extends into the middle hole of the air storage ring pad in the air nozzle thread column. The outlet end of the mist diffuser is connected with a mist ball. A gas channel is opened inside the connecting body. The gas channel is connected to the interior of the mist diffuser through branch channels. The ends of the gas channel are connected to the atomization control box through air valve channel components. The atomization control box is connected to the external air intake component. An acceleration ring and an air storage ring pad are provided inside the air nozzle thread column. The acceleration ring is a cylindrical structure with a through hole in the middle and multiple pores or tapered grooves around the through hole. The air storage ring pad is a cylindrical structure with a through hole in the middle. The diameter of the through hole in the air storage ring pad is larger than the diameter of the liquid inlet pipe. Preferably, the diameter of the through hole in the air storage ring pad is 0.2-0.5mm larger than the diameter of the liquid inlet pipe. The liquid inlet pipe is a corrosion-resistant stainless steel pipe or other metal pipe, and a hard plastic pipe can also be used. Preferably, the outer diameter of the liquid inlet pipe is 1.0-1.5mm and the inner diameter is 0.5-1.1mm. By setting the atomizing head assembly, multiple atomizers can be connected in series to achieve simultaneous spraying of multiple sprayers. The combination of the air nozzle threaded column, the acceleration ring and the air storage ring pad can accelerate the outflow of air and form a vacuum at the end of the liquid inlet pipe to carry out the reagent liquid spray. The diffuser and the mist crusher further disperse and refine the mist flow. The fixed liquid inlet adjustment column on the liquid pipe can be screwed into the liquid pipe to adjust the distance between the liquid inlet pipe and the spray nozzle, thereby achieving the function of adjusting the amount of mist from the atomizer.

[0017] A further improvement is that the glue-dropping sealing module includes a first drive component, a second drive component, a mounting seat, a glue-dropping component, a cover sheet picking and placing component, and a cover sheet storage box. The mounting seat is equipped with a glue-dropping component for dripping glue onto the sample glass slide and a cover sheet picking and placing component for taking out the cover sheet from the cover sheet storage box and placing it on the sample glass slide. The mounting seat can be moved along the X, Y, and Z axis directions under the drive of the first drive component, and the cover sheet storage box can be moved to directly below the cover sheet picking and placing component under the drive of the second drive component.

[0018] A further improvement is that a plurality of isolating members are arranged side by side in the cover sheet storage box, and the isolating members divide the interior of the cover sheet storage box into a plurality of storage chambers for storing cover sheets along the length direction. Elastic side panels are provided on the sides of the interior of the cover sheet storage box along the length direction, and the outer sides of the elastic side panels are connected to the sides of the adjacent cover sheet storage boxes through elastic members.

[0019] A further improvement is that the cover sheet picking and placing assembly includes multiple groups of suction cup assemblies arranged side by side, the suction cup assembly includes two suction cups of different heights, and the number and position of the suction cup assemblies correspond one-to-one to the number and position of the storage chambers in the cover sheet storage box.

[0020] During use, the cover sheet storage box is pushed to the bottom of the suction cup assembly under the action of the second drive assembly, and the suction cup assembly extends downward into the cover sheet storage box to absorb the cover sheet. When extracting it upward, the elastic side panels at the sides of the cover sheet storage box scrape the cover sheet. At the same time, the two suction cups in the suction cup assembly, one high and one low, exert a cover sheet scraping force to tear apart the cover sheets that are stuck together. This structure ensures that overlapping cover sheets will not be absorbed at the same time. After the suction cup absorbs the cover sheet and lifts it upward, the cover sheet storage box retracts under the drive of the second drive assembly, and the suction cup presses the absorbed cover sheet downward toward the sample slide. When the cover sheet is pressed downward, the suction cup absorbs the cover sheet with one side high and the other side low, so the air will be expelled when the cover sheet is slowly pressed from one end to the other. In this way, there will be no bubbles caused by air in the sealing glue, and the covering is fast, uniform, bubble-free and of good quality. Glue dispensing and cover slips are completed in one action: the glue dispensing needles and suction cups are distributed front and back on an X-axis drive component. That is, when multiple glue dispensing needles pass over the sample glass slide, glue is dispensed. At this time, the suction cups suck up multiple cover slips and press down just above the sample glass slide to complete the sealing. Multiple sample slides can be completed in one action, and glue dispensing and sealing are completed at the same time, with extremely fast sealing speed.

[0021] A further improvement is that the three-dimensional stacked model: the following modules are stacked on the left and right sides respectively: centrifugal liquid-spinning module, dyeing module, sealing module, high-temperature repair water bath module, cover cleaning module, incubation cover film module, pipetting module, etc., and the middle is a feeding module; the feeding module includes a material rack, a tray support, a lifting mechanism and a push-pull mechanism, and a plurality of tray supports for placing trays are provided inside the material rack from top to bottom. The tray support can move up and down under the drive of the lifting mechanism, and the push-pull mechanism is used in conjunction with the tray on the tray support to push the tray on the tray support to the incubation module, the dyeing module and other modules, and the tray at the incubation module, the dyeing module and other modules can be pulled back to the tray support. Through the setting, the tray is transported to the corresponding module height through the lifting mechanism, and then pushed out from the material rack to each module through the push-pull mechanism. After the work is completed, the tray is pulled back to the material rack. Multiple trays can circulate and work at each station at the same time. For example, the tray on the first layer of the material rack is sealing, the tray on the second layer is staining, the tray on the third layer is incubating antibodies, the tray on the fourth layer is repaired at high temperature, and the tray on the fifth layer is baking. That is, multiple trays are processing different work steps at the same time, and the work steps do not affect each other and can work simultaneously.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The device of the present invention has the following features in immunohistochemistry:

[0024] (1) Multiple stations on the assembly line work simultaneously, with 120-1200 sample slides and more than 200 bottles of antibody reagents loaded on the machine at one time;

[0025] (2) The reading board can be loaded and unloaded quickly and conveniently;

[0026] (3) The high temperature zone and the low temperature zone are independent and sealed working areas, and immersion high temperature repair is adopted at the same time, without the trouble of dry pieces, and the temperature is easy to control;

[0027] (4) The contaminated area and the clean area are independent and closed working areas, which do not affect each other; at the same time, the staining adopts the atomization method, which ensures uniform staining and good cleaning effect; the centrifugal method is used to remove the excess waste liquid from the stained sample slides at one time, which is highly efficient, leaves no dead corners, and is thoroughly cleaned; after the staining waste liquid is removed from the contaminated area, it is cleaned before entering the clean area for antibody incubation. The incubation adopts multiple elastic small cover templates, the whole cover film, and the whole automatic immersion and cleaning cover template to reduce background interference, make the cell layer of the sample preparation clearer, and increase work efficiency;

[0028] (5) The sample slides are sealed with a cover template only during antibody incubation. They are left open during staining, dewaxing, washing, and repair, allowing for more thorough staining, dewaxing, washing, and repair.

[0029] (6) HE staining, sealing, and immunohistochemistry are all independent modules that can be freely combined into different functional systems to achieve multifunctional and standardized production;

[0030] In terms of HE staining and sealing:

[0031] (1) The vacuum negative pressure ultrafine atomizer assembly realizes ultrafine and soft atomization dyeing and cleaning of multiple sample slides with one gas source, avoiding the cross contamination and poor stability problems of the immersion dyeing method (hanging basket + dye vat), and avoiding the problems of the smooth surface of the glass slide flowing away and the uneven condensation of the reagent into liquid beads in the drop dyeing method;

[0032] (2) One-time centrifugal removal of waste liquid avoids cell damage caused by suction or blowing away waste liquid, and is highly efficient;

[0033] (3) The glue-drip sealing module is used to wet-seal the coverslips in batches, so as to achieve wet sealing in the best state when xylene dries quickly, with good sealing quality, good effect and high work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0035] Figure 1 Schematic diagram of the overall structure of the multi-station assembly line for flat-type immunohistochemistry and HE atomization staining and sealing in Example 1;

[0036] Figure 2 Schematic diagram of the internal structure of the multi-station assembly line for flat-type immunohistochemistry and HE atomization staining and sealing in Example 1;

[0037] Figure 3 Schematic diagram of the structure of the film reading board in Example 1;

[0038] Figure 4 This is a schematic structural diagram of the tray in Example 1;

[0039] Figure 5 This is a schematic structural diagram of the dyeing module in Example 1;

[0040] Figure 6 This is a schematic diagram of the structure of the atomizer head assembly in the assembled state in Example 1;

[0041] Figure 7 Schematic diagram of the structure of the atomizing head assembly in Example 1;

[0042] Figure 8 This is an exploded view of the atomizing head assembly in Example 1;

[0043] Figure 9 This is a schematic structural diagram of the glue sealing module in Example 1;

[0044] Figure 10 This is a structural diagram of the cover sheet storage box and the second drive assembly in the connected state in Example 1;

[0045] Figure 11 This is a schematic structural diagram of the cover sheet storage box in Example 1;

[0046] Figure 12 This is a schematic diagram of the structure of the incubation module in Example 1;

[0047] Figure 13 This is a schematic structural diagram of the connection between the fixed plate and the cover mold bottom plate in Example 1;

[0048] Figure 14 This is a schematic structural diagram of the mist shield of the spray assembly in Example 1;

[0049] Figure 15 This is a schematic diagram of the structure of the acceleration ring, air storage ring pad and liquid inlet pipe when used together in Example 1.

[0050] Figure 16 Schematic diagram of the overall structure of the multi-station assembly line for stereoscopic immunohistochemistry and HE atomization staining and sealing in Example 2;

[0051] Figure 17 This is a structural diagram of the feeding module in Example 2;

[0052] Among them, the specific figures are marked as: shell 1, film reading plate 2, accommodating slot 3, hanging buckle 4, hook slot 5, upper baffle 6, film pressing plate 7, elastic handle head 8, handle slot 9, support foot fixing slot 10, tray 11, baffle 12, isolation plate 13, block 14, elastic blocking member 15, pulley 16, sliding conveying mechanism 17, staining module 18, driving mechanism 19, mounting frame 20, atomization control box 21, atomization head assembly 22, liquid inlet pipe 23, connecting body 24, gas channel 25, liquid inlet fixed adjustment column 26, air nozzle threaded column 27, tightening nut 28, mist expander 29, mist breaking ball 30, spray assembly mist shield 31 , centrifugal liquid disposal module 32, glue sealing module 33, second drive assembly 35, mounting seat 36, glue dripping assembly 37, cover sheet picking and placing assembly 38, suction cup assembly 39, suction cup 40, cover sheet storage box 41, isolation member 42, elastic side plate 44, mobile liquid adding module 45, incubation module 46, cover template assembly 47, drive device 48, fixing plate 49, cover template 50, cover template cleaning module 51, high temperature repair water bath module 52, feeding module 53, material rack 54, tray support 55, lifting mechanism 56, push-pull mechanism 57, movable isolation assembly 58, elastic buffer assembly 59, acceleration ring 60, air storage ring pad 61. DETAILED DESCRIPTION

[0053] Example 1

[0054] Example 1 of the present invention discloses a multi-station assembly line for flat-type immunohistochemistry and HE atomization staining and sealing, such as Figure 1 and Figure 2 As shown, it includes a housing 1, in which an incubation module 46, a cover template cleaning module 51, a mobile liquid adding module 45, a tray 11, a dyeing module 18, a centrifugal liquid removal module 32, a glue sealing module 33 and a high-temperature repair water bath module 52 are arranged;

[0055] like Figure 3 and Figure 4 As shown, a plurality of reading plates 2 are evenly inserted on the top of the tray 11, and the ends of the reading plates 2 are fixed to the top of the tray 11. The top of the reading plates 2 is evenly provided with rows of receiving grooves 3 for mounting sample slides, and the ends of the sample slides are fixed in the receiving grooves 3. The tray 11 and the reading plates 2 are hollow structures. The cooperation of the reading plates 2 and the tray 11 facilitates loading and unloading, and 120-1200 sample slides can be loaded on the machine at a time. In addition, the tray 11 and the reading plates 2 are hollow structures, which facilitates the removal of waste liquid during staining.

[0056] The two opposite sides of the reading board 2 are respectively provided with a hanging buckle 4 and a hook groove 5, and the adjacent two reading boards 2 are fixed together by the hanging buckle 4 and the hook groove 5. When in use, the hanging buckle 4 of the front reading board 2 is matched with the hook groove 5 of the rear reading board 2, so that multiple reading boards 2 can be connected to form a whole, which is convenient for installing it on the tray 11 or taking it out from the tray 11. The connection between the four adjacent receiving grooves 3 on the reading board 2 is provided with an upper baffle 6 for limiting the top of the sample glass slide in the receiving groove 3. The reading board 2 is rotatably provided with a pressing plate 7 at both ends along the length direction. The pressing plate 7 is provided with an elastic handle head 8 at both ends along the length direction. The reading board 2 is provided with a The elastic handle head 8 is engaged with the handle slot 9. By engaging the elastic handle head 8 into the handle slot 9, the pressing plate 7 can be pressed down to the top of the sample glass in the receiving slot 3. When in use, each sample glass is inserted into the receiving slot 3 on the reading plate 2 until the end of the sample glass is inserted under the upper baffle 6. After all the sample glass slides are inserted, the pressing plate 7 is rotated until the elastic handle head 8 on the pressing plate 7 is engaged with the handle slot 9. At this time, the pressing plate 7 is pressed on the other end of the sample glass, so that the sample glass can be fixed in the receiving slot 3 on the reading plate 2. Support foot fixing grooves 10 are formed at the four corners of the top of the reading plate 2, and support feet are provided at the four corners of the bottom of the reading plate 2. Through the setting, multiple reading plates 2 can be stacked together for transportation, which is convenient for moving multiple reading plates 2. The receiving slot 3 and the pressing plate 7 are both provided with multiple small cylindrical pillars, which are convenient for pressing and fixing the sample glass by point contact, and convenient for removing waste liquid during subsequent staining.

[0057] Among them, the top of the tray 11 is provided with baffles 12 on the front and back sides, and a plurality of isolation plates 13 arranged side by side with the baffles 12 are provided on the top of the tray 11. The baffles 12 and isolation plates 13 divide the top of the tray 11 into multiple rows of independent cavities for installing the reading boards 2. The top of the tray 11 is provided with a stopper 14 and an elastic stopper 15 at both ends of the independent cavity for limiting and fixing the ends of the reading boards 2. The bottom of the tray 11 is provided with a pulley 16 to reduce friction resistance when the tray 11 moves between modules. When in use, the above structure can fix the reading boards 2 placed on the tray 11.

[0058] The staining module 18 and the glue sealing module 33 are both located above the tray 11, and the centrifugal liquid-spinning module 32 is located below the tray 11, and it can drive the tray 11 to rotate in the horizontal direction, centrifugally remove the staining reagent on the sample slide or spin the liquid while spraying the cleaning liquid. The outside of the staining module 18 is provided with an incubation module 46 and a high-temperature repair water bath module 52. The tray 11 can be moved to the incubation module 46 and the high-temperature repair water bath module 52 under the drive of the sliding conveying mechanism 17, and the staining module 18 and the incubation module 4 6. The high-temperature repair water bath modules 52 are isolated from each other by movable isolation components 58; four cylinder lifting mechanisms equipped with guide bars and push rods are provided at the four corners of the bottom plate of the centrifugal liquid rejection module 32. The cylinder lifting mechanism includes guide bars and push rods. When the tray 11 moves in and out, the guide bars and push rods rise, and the tray 11 can slide from the guide bars to other modules. The guide bars act as a temporary bridge, and the push rods push the elastic seesaw block to the lower end. At this time, the two ends of the elastic seesaw block are flat on the turntable surface, so that the tray 11 can move in and out of the turntable. The process of moving the tray 11 to other modules is as follows: a silicon steel iron sheet is provided in the middle of both ends of the tray 11, and two electromagnets are provided on the outside of the atomization control box 21 in the middle of the sliding conveying mechanism 17. The electromagnets are fixed on the sliding rod of the lifting cylinder. When the tray 11 is to be moved, the cylinder extends to make the electromagnet contact with the silicon steel iron sheet on the tray 11 and energize and attract it. Then, under the push of the sliding conveying mechanism 17, the tray 11 is pushed to other modules, and then the tray 11 is fixed to the bracket by the elastic clip on the bracket. Then, the rotating motor in the centrifugal liquid-spinning module 32 drives the tray 11 to rotate horizontally, and the excess staining reagent on the sample slide is centrifugally discarded. A group of atomizing heads among the multiple atomizing head assemblies 22 in the staining module 18 can also spray cleaning liquid onto the sample slide for cleaning.

[0059] like Figure 5-8 As shown, the dyeing module 18 includes a plurality of atomizing head assemblies 22, an atomizing control box 21, a mounting frame 20, a spray assembly mist shield 31 and a driving mechanism 19. A plurality of atomizing head assemblies 22 are mounted side by side at the lower portion of the mounting frame 20, and an atomizing control box 21 is provided at the upper portion of the mounting frame 20. The mounting frame 20 can be moved along the X-axis and Y-axis directions under the drive of the driving mechanism 19, and the spray assembly mist shield 31 is correspondingly mounted at the bottom end of each atomizing head assembly 22 (as shown in FIG. Figure 14As shown), the spray assembly mist shield 31 can block the mist liquid in the area of ​​several sample sheets of the current spray to prevent the mist liquid from spreading and contaminating other components. The atomizing head assembly 22 includes multiple liquid inlet pipes 23, a connecting body 24, a liquid inlet fixed adjustment column 26, an air nozzle threaded column 27, a tightening nut 28, a mist diffuser 29 and a mist crushing ball 30. Multiple liquid inlet pipes 23 are arranged side by side on the connecting body 24. The outer side of the upper part of the liquid inlet pipe 23 is threadedly connected to the connecting body 24 through the liquid inlet fixed adjustment column 26. A mist diffuser 29 is provided at the bottom of the connecting body 24 corresponding to the liquid inlet pipe 23. The upper thread of the mist diffuser 29 An air nozzle threaded column 27 is connected, the upper part of the air nozzle threaded column 27 is arranged inside the connecting body 24, the lower part of the liquid inlet pipe 23 passes through the air nozzle threaded column 27 and extends into the interior of the mist diffuser 29, the outlet end of the mist diffuser 29 is connected with a mist crushing ball 30, a gas channel 25 is opened inside the connecting body 24, the gas channel 25 is connected to the interior of the mist diffuser 29 through branch channels, the ends of the gas channel 25 are connected to the atomization control box 21 through the air valve channel assembly, the atomization control box 21 is connected to the external air intake assembly, the air nozzle threaded column 27 is provided with an acceleration ring 60 and a gas storage ring pad 61, as shown Figure 15 As shown, the acceleration ring 60 is a cylindrical structure with a through hole in the middle, and a plurality of pores or conical inclined grooves are provided around the through hole. The air storage ring pad 61 is a cylindrical structure with a through hole in the middle. The diameter of the through hole in the air storage ring pad 61 is larger than the diameter of the liquid inlet pipe. Preferably, the diameter of the through hole in the air storage ring pad 61 is 0.2-0.5mm larger than the diameter of the liquid inlet pipe. The liquid inlet pipe 23 is a corrosion-resistant stainless steel pipe or other metal pipe, and a hard plastic pipe can also be selected. Preferably, the outer diameter of the liquid inlet pipe 23 is 1.0-1.5mm, and the inner diameter is 0.5-1.1mm. By setting up the atomizing head assembly 22, multiple atomizers can be connected in series to achieve simultaneous spraying of multiple sprayers. The combined use of the air nozzle threaded column 27, the acceleration ring and the air storage ring pad can accelerate the outflow of air to form a vacuum at the end of the liquid inlet pipe 23 and carry out the reagent liquid for spraying. The mist expander 29 and the mist crushing ball 30 further disperse and refine the mist flow. The liquid inlet fixed adjustment column 26 on the liquid pipe 23 can be screwed into the liquid pipe 23 to adjust the distance between the liquid inlet pipe 23 and the spray nozzle 31, thereby realizing the function of adjusting the amount of mist of the atomizer.

[0060] like Figure 9-11As shown, the glue sealing module 33 includes a first driving component, a second driving component 35, a mounting base 36, a glue dripping component 37, a cover sheet taking and placing component 38, and a cover sheet storage box 41. The mounting base 36 is equipped with a glue dripping component 37 for dripping glue onto the sample glass slide and a cover sheet taking and placing component 38 for taking out the cover sheet in the cover sheet storage box 41 and placing it on the sample glass slide. The mounting base 36 can be moved along the X, Y, and Z axis directions under the drive of the first driving component, and the cover sheet storage box 41 can be moved to the bottom of the cover sheet taking and placing component 38 under the drive of the second driving component 35; the cover sheet storage box 41 1 is provided with a plurality of isolators 42 side by side, and the isolators 42 divide the interior of the cover sheet storage box 41 into a plurality of storage chambers for storing cover sheets along the length direction. The side surfaces of the cover sheet storage box 41 along the length direction are provided with elastic side panels 44, and the outer sides of the elastic side panels 44 are connected to the side surfaces of the adjacent cover sheet storage boxes 41 through elastic members; the cover sheet picking and placing assembly 38 includes a plurality of groups of suction cup assemblies 39 arranged side by side, and the suction cup assembly 39 includes two suction cups 40 of different heights. The number and positions of the suction cup assemblies 39 correspond one to one with the number and positions of the storage chambers in the cover sheet storage box 41. During use, the cover storage box 41 is pushed to the bottom of the suction cup assembly 39 under the action of the second drive assembly 35, and the suction cup assembly 39 extends downward into the cover storage box 41 to absorb the cover. When extracting upward, the elastic side panels 44 at the sides of the cover storage box 41 scrape the cover. At the same time, the two suction cups 40 in the suction cup assembly 39, one high and one low, apply a cover scraping force to tear apart the cover sheets that are stuck together. This structure ensures that overlapping cover sheets are not absorbed at the same time. After the suction cup 40 absorbs the cover sheet and lifts it up, the cover storage box 41 retracts under the drive of the second drive assembly 35, and the suction cup 40 presses the absorbed cover sheet downward toward the sample slide. When the cover sheet is pressed downward, the suction cup 40 absorbs the cover sheet with one side high and the other side low, so the cover sheet is slowly pressed from one end to the other end to drive out the air, so that there will be no bubbles caused by air in the sealing glue, and the covering is fast, uniform, bubble-free and of good quality. Glue dispensing and cover slips are completed in one action: the glue dispensing needles and suction cups are distributed front and back on an X-axis drive assembly. That is, when multiple glue dispensing needles pass over the sample glass slide, glue is dispensed. At this time, the suction cups suck up multiple cover slips and press down just above the sample glass slide to complete the sealing. This allows multiple sample slides to be dispensed and sealed simultaneously in one action, with extremely fast sealing speed.

[0061] like Figure 12 and Figure 13As shown, the incubation module 46 can accommodate the tray 11, and a cover template assembly 47 and a driving device 48 are provided on the top of the incubation module 46. The cover template assembly 47 includes a fixed plate 49 and a plurality of cover templates 50. The fixed plate 49 can move along the Y-axis and Z-axis directions under the drive of the driving device 48, and the fixed plate 49 is located above the tray 11. The plurality of cover templates 50 are installed at the bottom of the fixed plate 49. A long sample addition slot for the sample liquid to pass through is formed on the fixed plate 49. The sample addition slot is alternately distributed corresponding to the plurality of cover templates 50. The cover template 50 includes an upper cover plate and a lower cover plate. A heating plate is provided in the cavity in the middle of the lower cover plate. One of the two pillars on the upper cover plate is provided with a through hole for passing a circuit connected to the heating plate. The bottom surface of the lower cover plate is a flat and smooth surface with a liquid-blocking frame, and an elastic buffer assembly is provided between the lower cover plate and the fixed plate, so that the cover template 50 moves downward and contacts the sample. The contact of the glass slides forms an elastic contact force to avoid damage to the sample slides. A cover template cleaning module 51 that can move up and down is provided below the incubation module 46, and a mobile liquid adding module 45 is provided above the incubation module 46. The driving device 48 drives the fixed plate 49 to move the sample adding slit to the area where the cells are coated on the sample slide. The reagent is added to the sample slide on the tray 11 by moving the liquid adding module 45, and then the cover template 50 is controlled to move along the Y axis so that the bottom plate is facing the area where the cells are coated on the sample slide. The cover template 50 is then controlled to move along the Z axis so that the cover template 50 is gently pressed downward to seal the antibody reagent between the cover template 50 and the sample slide. After incubation, the cover template 50 rises, and after the tray 11 is pulled away, the cover template 50 is lowered into the cleaning liquid tank of the cover template cleaning module 51 to immerse and repeatedly move up and down to oscillate and clean the reagent on the cover template 50.

[0062] The movable isolation assembly 58 includes a frame, a lower baffle is provided at the lower part of the frame, a passage for the tray 11 to pass through is formed at the upper part of the frame, and a movable baffle is provided at the upper part of the frame. The movable baffle can be moved up and down by the cylinder to open or block the passage.

[0063] The high-temperature repair water bath module 52 includes a repair pool, a basket guide rail and a lifting device. The lifting device drives the basket guide rail upward, and the tray 11 enters the basket guide rail. The lifting device then drives the basket guide rail downward, and the sample slides on the tray 11 are immersed in the repair liquid in the repair pool. The repair liquid is heated to the set temperature. After the repair is completed, the basket guide rail is lifted to move the sample slides.

[0064] Example 2

[0065] Example 2 of the present invention discloses a multi-station assembly line for three-dimensional immunohistochemistry and HE atomization staining and sealing, which has the same basic features as the multi-station assembly line for flat-type immunohistochemistry and HE atomization staining and sealing in Example 1, and the two have the following distinguishing technical features: Figure 16 and Figure 17As shown, the right side is divided from bottom to top into a centrifugal liquid-spinning module, a staining module, and a sealing module; the middle is a feeding module 53; the left side is divided from bottom to top into a high-temperature repair water bath module, a cover cleaning module, an incubation and film-covering module, and a pipetting module, with each module layered and isolated into independent spaces. The feeding module 53 includes a material rack 54, a tray support 55, a lifting mechanism 56, and a push-pull mechanism 57. The material rack 54 is provided with a plurality of tray supports 55 for placing trays 11 from top to bottom. The tray supports 55 can move up and down under the drive of the lifting mechanism 56. The push-pull mechanism 57 cooperates with the tray 11 on the tray support 55 to push the tray 11 on the tray support 55 to the incubation module 46, the staining module 18, and other modules, and can pull the tray 11 at the incubation module 46, the staining module 18, and other modules back to the tray support 55. Through the setting, the tray 11 is transported to the corresponding module height through the lifting mechanism 56, and then the tray 11 is pushed out from the material rack 54 to each module through the push-pull mechanism 57. After the work is completed, the tray 11 is pulled back to the material rack 54. Multiple trays 11 can circulate and work at each work station at the same time. For example, the tray 11 on the first layer of the material rack 54 is sealing, the tray 11 on the second layer is staining, the tray 11 on the third layer is incubating with antibodies, the tray 11 on the fourth layer is repaired at high temperature, and the tray 11 on the fifth layer is baking. That is, multiple trays 11 are processing different work steps at the same time, and the work steps do not affect each other and can work simultaneously.

[0066] The device of the present invention has the following features in immunohistochemistry:

[0067] (1) Multiple stations on the assembly line work simultaneously, with 120-1200 sample slides and more than 200 bottles of antibody reagents loaded on the machine at one time;

[0068] (2) The loading and unloading of the film reading board 2 is convenient and fast;

[0069] (3) The high temperature zone and the low temperature zone are independent and sealed working areas, and immersion high temperature repair is adopted to reduce the trouble of dry slices and make the temperature easy to control;

[0070] (4) The contaminated area and the clean area are independent and closed working areas, which do not affect each other; at the same time, the staining adopts the atomization method, which ensures uniform staining and good cleaning effect; the centrifugal method is used to remove the excess waste liquid from the stained sample slides at one time, which is highly efficient, leaves no dead corners, and is thoroughly cleaned; after the staining waste liquid is removed from the contaminated area, it is cleaned before entering the clean area for antibody incubation. The incubation adopts multiple elastic small cover templates, the whole cover film, and the whole automatic immersion and cleaning cover template, without background interference, the cell layer of the sample preparation is clearer, and the work efficiency is high;

[0071] (5) The sample slides are sealed with a cover template only during antibody incubation. They are left open during staining, dewaxing, washing, and repair, allowing for more thorough staining, dewaxing, washing, and repair.

[0072] (6) HE staining, sealing, and immunohistochemistry are all independent modules that can be freely combined into different functional systems, and freely combined into flat and three-dimensional types to achieve multifunctional and standardized production;

[0073] In terms of HE staining and sealing:

[0074] (1) The vacuum negative pressure ultrafine atomizer assembly realizes ultrafine and soft atomization dyeing and cleaning of multiple sample slides with one gas source, avoiding the cross contamination and poor stability problems of the immersion dyeing method (hanging basket + dye vat), and avoiding the problems of the smooth surface of the glass slide flowing away and the uneven condensation of the reagent into liquid beads in the drop dyeing method;

[0075] (2) One-time centrifugal removal of waste liquid avoids cell damage caused by suction or blowing away waste liquid, and is highly efficient;

[0076] (3) The glue sealing module 33 is used to wet-seal the coverslips in batches, so that the wet sealing can be performed in the optimal state and time when xylene is drying quickly. More than 10 coverslips can be sealed at the same time by glue-drip sealing in one action, with good sealing quality, good effect and high work efficiency.

[0077] The above examples are used to illustrate the present invention, which are only used to help understand the present invention and are not intended to limit the present invention. Those skilled in the art can make several simple deductions, modifications or substitutions based on the concept of the present invention.

Claims

1. A multi-station production line for immunohistochemistry and HE atomization staining and sealing, characterized in that: The system comprises a housing, in which an incubation module, a cover template cleaning module, a mobile liquid adding module, a tray, a dyeing module, a centrifugal liquid-spinning module, a glue-drip sealing module and a high-temperature repair water bath module are arranged; A plurality of reading plates are evenly inserted on the top of the tray, and the ends of the reading plates are fixed to the top of the tray. Rows of receiving grooves for mounting sample slides are evenly arranged on the top of the reading plates, and the ends of the sample slides are fixed in the receiving grooves. The tray and the reading plates are hollow structures. The dyeing module and the glue sealing module are both located above the tray, the centrifugal liquid-spinning module is located below the tray, and can drive the tray to rotate in the horizontal direction. The incubation module and the high-temperature repair water bath module are provided on the outside of the dyeing module. The tray can be moved to the incubation module and the high-temperature repair water bath module under the drive of the sliding conveying mechanism, and the dyeing module is isolated from the incubation module and the high-temperature repair water bath module by a movable isolation component. The tray can be accommodated inside the incubation module, and a cover template assembly and a driving device are provided on the top of the incubation module, and the cover template assembly includes a fixed plate and multiple cover templates, and the fixed plate can be moved along the Y-axis and Z-axis directions under the drive of the driving device, and a plurality of liquid-adding long slots are opened on the fixed plate, and the liquid-adding long slots and the cover templates are arranged alternately and symmetrically, the fixed plate is located above the tray, and multiple cover templates are installed at the bottom of the fixed plate, and the cover template includes an upper cover plate and a lower cover plate, and a heating plate is provided in the cavity in the middle of the lower cover plate, and one of the two pillars on the upper cover plate is provided with a through hole for passing a circuit connected to the heating plate, and the bottom surface of the lower cover plate is a flat and smooth surface with a liquid-blocking frame, and an elastic buffer assembly is provided between the lower cover plate and the fixed plate; A cover template cleaning module that can move up and down is provided below the incubation module, and the movable liquid adding module is provided above the incubation module.

2. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 1, characterized in that: Baffles are provided on the front and rear sides of the top of the tray, and a plurality of isolation plates are provided on the top of the tray in parallel with the baffles. The baffles and the isolation plates divide the top of the tray into multiple rows of independent cavities for installing reading boards. Baffles and elastic blocking members for limiting and fixing the ends of the reading boards are respectively provided at both ends of the independent cavities on the top of the tray, and a pulley is provided at the bottom of the tray.

3. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 1 or 2, characterized in that: The reading board is provided with a hanging buckle and a hook groove on the opposite sides respectively, and the two adjacent reading boards are fixed together by the hanging buckle and the hook groove, and the connection between the four adjacent receiving grooves on the reading board is provided with an upper baffle for limiting the top of the sample glass slide in the receiving groove, and the reading board is rotatably provided with a pressing plate at both ends along the length direction, and the pressing plate is provided with an elastic handle head at both ends along the length direction respectively, and the reading board is provided with a handle groove engaged with the elastic handle head. By engaging the elastic handle head into the handle groove, the pressing plate can be pressed down to the top of the sample glass slide in the receiving groove.

4. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 3, characterized in that: Support foot fixing grooves are formed at the four corners of the top of the reading board, and support feet are provided at the four corners of the bottom of the reading board.

5. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 1, characterized in that: The dyeing module includes a plurality of atomizing head assemblies, an atomizing control box, a mounting frame, a spray assembly mist shield and a driving mechanism, a plurality of the atomizing head assemblies are installed side by side at the lower part of the mounting frame, the atomizing control box is provided on the upper part of the mounting frame, the mounting frame can move along the X-axis and Y-axis directions under the drive of the driving mechanism, the spray assembly mist shield is correspondingly installed at the bottom end of each of the atomizing head assemblies, the atomizing head assembly includes a plurality of liquid inlet pipes, a connecting body, a liquid inlet fixed adjustment column, an air nozzle threaded column, a tightening nut, a mist diffuser and a mist crushing ball, a plurality of the liquid inlet pipes are arranged side by side on the connecting body, the outer side of the upper part of the liquid inlet pipe is threadedly connected to the connecting body through the liquid inlet fixed adjustment column, a mist diffuser is provided at the position corresponding to the liquid inlet pipe at the bottom of the connecting body, and the upper threaded connection of the mist diffuser The air nozzle threaded column is connected, the upper part of the air nozzle threaded column is arranged inside the connecting body, the lower part of the liquid inlet pipe passes through the air nozzle threaded column and extends into the interior of the mist diffuser, the outlet end of the mist diffuser is connected and is provided with a mist ball, a gas channel is opened inside the connecting body, the gas channel is connected to the interior of the mist diffuser through branch channels respectively, the ends of the gas channel are connected to the atomization control box through air valve channel assemblies respectively, the atomization control box is connected to the external air intake assembly, an acceleration ring and an air storage ring pad are provided inside the air nozzle threaded column, the acceleration ring is a cylindrical structure with a through hole in the middle, a plurality of pores or conical inclined grooves are provided around the through hole, the air storage ring pad is a cylindrical structure with a through hole in the middle, and the diameter of the through hole in the air storage ring pad is larger than the diameter of the liquid inlet pipe.

6. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 1, characterized in that: The glue-dropping sealing module includes a first drive component, a second drive component, a mounting seat, a glue-dropping component, a cover sheet picking and placing component, and a cover sheet storage box. The mounting seat is equipped with a glue-dropping component for dripping glue onto the sample glass slide and a cover sheet picking and placing component for taking out the cover sheet from the cover sheet storage box and placing it on the sample glass slide. The mounting seat can be moved along the X, Y, and Z axis directions under the drive of the first drive component, and the cover sheet storage box can be moved to directly below the cover sheet picking and placing component under the drive of the second drive component.

7. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 6, characterized in that: A plurality of isolating members are arranged side by side in the cover sheet storage box, and the isolating members divide the interior of the cover sheet storage box into a plurality of storage chambers for storing cover sheets along the length direction. Elastic side panels are provided on the sides of the interior of the cover sheet storage box along the length direction, and the outer sides of the elastic side panels are connected to the sides of the adjacent cover sheet storage boxes through elastic members.

8. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 7, characterized in that: The cover sheet picking and placing assembly includes multiple groups of suction cup assemblies arranged side by side, and the suction cup assembly includes two suction cups of different heights. The number and position of the suction cup assemblies correspond one-to-one to the number and position of the storage chambers in the cover sheet storage box.

9. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 1, characterized in that: A feeding module is provided between the dyeing module and the incubation module. The feeding module includes a material rack, a tray support, a lifting mechanism and a push-pull mechanism. A plurality of tray supports for placing trays are provided inside the material rack from top to bottom. The tray support can move up and down driven by the lifting mechanism. The push-pull mechanism cooperates with the tray on the tray support to push the tray on the tray support to the incubation module and the dyeing module, and can pull the tray at the incubation module and the dyeing module back to the tray support.

10. The multi-station assembly line for immunohistochemistry and HE atomization staining and sealing according to claim 1, characterized in that: Each module can be freely assembled into a HE stainer, a coverslip machine, an all-in-one HE staining and coverslip machine, a slice baking machine, an immunohistochemistry stainer, and an all-in-one immunohistochemistry and HE atomization staining and coverslip machine.

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