A method for promoting seasonal conversion of tobacco organic matter by combining organic fertilizer with oxygenation

Abstract

The invention discloses a method for promoting the conversion of tobacco organic matter in the current season by combining organic fertilizer with oxygenation. After the tobacco seedlings are transplanted on the ridge, two deep holes are opened at a position 8-12 cm away from the root system of the tobacco seedlings on the ridge, the bottom of the deep hole contacts the base fertilizer on the ridge, and calcium peroxide is placed in one of the deep holes as a chemical oxygenation method, and the other deep hole is not treated as a physical oxygenation method, and the base fertilizer is an organic fertilizer. The present invention can significantly improve the mineralization rate of organic fertilizer by combining organic fertilizer with oxygenation, thereby accelerating the release of nutrient elements, promoting the production of beneficial metabolites in the soil, and significantly improving the effect of the middle tobacco leaves, and the upper tobacco leaves are improved.

Description

Technical Field

[0001] The invention relates to a method for promoting the conversion of tobacco organic matter in season by combining organic fertilizer with oxygenation, and belongs to the technical field of tobacco planting. Background Art

[0002] Organic fertilizer is a fundamental way to improve the efficiency of agricultural land and increase the utilization of agricultural waste resources. Guizhou Province, the second largest tobacco-producing area in the country, has been using organic fertilizer to maintain arable land, prevent degradation and improve quality in recent years.

[0003] Most of the nutrients in organic fertilizers are organic carbon and organic nitrogen, and organic carbon and nitrogen must be mineralized into inorganic carbon and nitrogen before they can be absorbed and utilized by crops. After organic fertilizers are applied to the soil, the mineralization of organic carbon and nitrogen is completed through a series of physical, chemical and biological processes. Therefore, all factors that affect the physical, chemical and biological aspects of the soil will inevitably affect the mineralization process and mineralization rate of organic carbon and nitrogen. In addition to the characteristics of the organic fertilizer itself, these factors also include temperature, moisture, and soil physical and chemical properties such as soil texture. Generally speaking, the annual mineralization rate of soil organic matter in northern China is 3.3-6.9% (Wang Wenshan, Wang Weimin, Cai Yadian, Zhang Meirong. Research on the mineralization rate of organic matter in farmland soil in Beijing. 1994-2022 China Academic Journal Electronic Publishing House, doi: 10.19336.). Different types of organic fertilizers have different mineralization rates of carbon and nitrogen, among which the mineralization rates of carbon and nitrogen in cow dung organic fertilizer are 22.24% and 19.61%, respectively, in chicken manure organic fertilizer, 51.04% and 41.46%, respectively, and in pig manure organic fertilizer, 37.03% and 22.16%, respectively (Zhou Bo, Gao Jiajia, Zhou Jianbin. Study on the mineralization characteristics of carbon and nitrogen in different types of organic fertilizers. Journal of Plant Nutrition and Fertilizer, 2012, 18(2): 366-373.). It can be seen that there are many factors affecting the mineralization of organic fertilizer nutrients, and the fertilizer effect is relatively lagging.

[0004] However, within 30 days after transplanting, the tobacco plant absorbs little nutrients, and then it rises sharply. The period of large-scale absorption of N and K is 31-70 days after planting. The N and K absorption in these 40 days account for 61.4% and 52.9% of the total absorption of the mature plant, respectively (Xie Yunbo, Feng Wenqiang, Zeng Xiangzhong, Qin Yusheng, Yu Hua, Cheng Shixi, Guo Shiping, Luo Dingqi. Research progress on the nutrient demand law and fertilization technology of flue-cured tobacco. Anhui Agricultural Science, 2010, 38(21): 11115-11119.). From the nutrient absorption curve of tobacco plants, it can be seen that with the cluster as the boundary, a large amount of nitrogen fertilizer should be applied in the early stage to prevent nitrogen deficiency (rich when less), and excessive nitrogen should be prevented in the later stage to prevent greed for green and late maturity (poor when old) (Zeng Rui, Wang Gang, Gui Kun. Discussion on the nutrient demand law and fertilization method of tobacco. Modern Agricultural Science and Technology, 2017, 19: 49-50.). It can be seen that after flue-cured tobacco is transplanted, the fertilizer demand is high in the short term. Therefore, in flue-cured tobacco cultivation, there are many factors affecting the mineralization of organic fertilizer nutrients, and the fertilizer effect is relatively lagging, which is inconsistent with the characteristics of tobacco production cycle being short and the high fertilizer demand in a short period of time. It has also become the main reason why organic fertilizers are "popular but not popular" in tobacco-growing areas in recent years. Therefore, accelerating the mineralization of nutrients after organic fertilizers are applied to the soil has important guiding significance for the efficient and reasonable application of organic fertilizers.

[0005] At present, the research on improving the oxygen environment of crop rhizosphere by using oxygenation technology is still in its infancy. Previous studies have focused on optimizing the effects of oxygenation technology parameters on crops and fish farming under water mode. Given the complexity of soil, there are few reports on the application of organic fertilizer combined with oxygenation to promote the transformation of soil organic matter in the season. Rhizosphere oxygenation technology is an emerging agricultural technology, and the rational application of organic fertilizer is the trend of tobacco development. The combination of the two on tobacco promotion, soil nutrient transformation, and the mechanism of action of related soil microorganisms needs to be studied and solved. Summary of the invention

[0006] Based on the above, the present invention provides a method for promoting the conversion of tobacco organic matter in season by combining organic fertilizer with oxygenation, which can effectively promote the rapid conversion of organic matter and meet the fertilizer needs of tobacco growth, thereby improving the quality indicators such as agronomic traits of flue-cured tobacco.

[0007] The technical scheme of the present invention is: a method for promoting the conversion of tobacco organic matter in season by combining organic fertilizer with oxygenation. After the tobacco seedlings are transplanted on the ridge, two deep holes are opened on the ridge at a position 8-12 cm away from the roots of the tobacco seedlings, and the bottom of the deep holes is in contact with the base fertilizer on the ridge. Calcium peroxide is placed in one of the deep holes as a chemical oxygenation method, and the other deep hole is not treated as a physical oxygenation method. The base fertilizer is organic fertilizer.

[0008] Preferably, deep holes are opened during the vigorous growth period after the tobacco seedlings are transplanted to carry out oxygenation treatment.

[0009] Preferably, for the ridge body on which organic fertilizer is applied as base fertilizer before transplanting, two deep holes are symmetrically opened on the ridge surface on both sides of the tobacco seedlings.

[0010] Preferably, for the ridge body where organic fertilizer is applied in holes as base fertilizer after transplanting, two deep holes are opened on the ridge surface on one side of the tobacco seedlings and are respectively located on both sides of the organic fertilizer application hole.

[0011] Preferably, the calcium peroxide is 50% calcium peroxide.

[0012] Preferably, the depth of the two deep holes is 8 cm, and the holes are punched using a conventional transplanting puncher. The amount of 50% calcium peroxide added is 35-45 g.

[0013] Preferably, the organic fertilizer includes cow dung organic fertilizer, oil cake organic fertilizer, distiller's grains organic fertilizer or biological organic fertilizer.

[0014] The beneficial effect of the present invention is that the organic fertilizer combined with the perforation aeration + calcium peroxide aeration measures of the present invention has different degrees of improvement on the yield, quality, soil organic matter mineralization rate and soil microbial Shannon index of tobacco leaves, especially the aroma quality, aroma volume and aftertaste of the upper tobacco leaves are significantly increased, and the impurities are significantly reduced. Among them, the perforation + 50% calcium peroxide calcium peroxide mixed aeration measures have the best effect on improving the yield of tobacco leaves and the quality of middle tobacco leaves, and have a significant effect on increasing the aroma quality of middle tobacco leaves, with moderate strength and smoke concentration, reduced irritation and increased aftertaste. And it can significantly promote the mineralization of soil organic matter and soil microbial diversity. The perforation aeration has the most obvious effect on the quality promotion of upper tobacco leaves. In general, organic fertilizer combined with aeration can significantly increase the mineralization rate of organic fertilizer, thereby accelerating the release of nutrients, promoting the production of beneficial metabolites in the soil, and significantly improving the middle tobacco leaves, and the upper tobacco leaves are improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Schematic diagram of punching holes for strip application of organic fertilizer;

[0016] Figure 2 Field test of cow dung organic fertilizer combined with oxygenation (basal fertilizer strip application);

[0017] Figure 3 Field test of cow dung organic fertilizer combined with oxygenation (hole fertilizer strip application);

[0018] Figure 4 Cow dung organic fertilizer combined with different oxygenation methods for tobacco plant growth;

[0019] Figure 5 Soil microbial activity of cow dung organic fertilizer combined with different aeration methods;

[0020] Figure 6Growth of cow dung organic fertilizer under different oxygenation times (40 days - left; 80 days - right);

[0021] Figure 7 Agronomic traits of cow dung organic fertilizer under different oxygenation times (40 days - left; 80 days - right);

[0022] Figure 8 Cow dung organic fertilizer combined with pump oxygenation optimal oxygenation time tobacco growth;

[0023] Fig. 9 Optimal aeration time for cow dung organic fertilizer combined with pump aeration, soil nutrition-related enzyme activities;

[0024] Fig.10 Optimal aeration time for cow dung organic fertilizer combined with pump aeration, soil redox-related enzyme activities;

[0025] Fig.11 The optimal aeration time of cow dung organic fertilizer combined with pump aeration; Enzyme activities related to tobacco leaf quality metabolites in the soil; DETAILED DESCRIPTION

[0026] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.

[0027] The field test of organic fertilizer combined with oxygenation was conducted in 2022 in a typical tobacco-growing area in Bijie City, the largest tobacco-growing area in Guizhou Province. A relatively sticky tobacco-growing soil was selected to evaluate the oxygenation effect. The organic fertilizers used are cow dung organic fertilizer and distiller's grains organic fertilizer, which are the most commonly used in flue-cured tobacco producing areas. A randomized block experiment design was adopted, as shown in Table 1. Punching + chemical oxygenation Ca2O2 was used as the treatment, the conventional production method (no oxygenation) was used as the control (CK), and single punching and chemical oxygenation were used as oxygenation controls. Oxygenation treatments were carried out in strip application and hole application, respectively. The effects of different oxygenation methods were compared and verified by investigating, counting and analyzing indicators such as soil organic matter mineralization rate, microbial activity, tobacco leaf output value and yield.

[0028] (1) The conversion rate of organic fertilizer in the current season is expressed by the mineralization rate of organic matter. The mineralization rate of organic fertilizer refers to the speed at which organic compounds in the soil are decomposed into inorganic salts by soil microorganisms. The determination method is as follows:

[0029] Organic matter mineralization rate Rm (mg.g -1 .d -1 )=(OMB-OMA) / D

[0030] Among them, OMB is the soil organic matter content before oxygenation, OMA is the soil organic matter content after oxygenation, and D is the number of days of oxygenation.

[0031] (2) The activity of soil microbial communities is represented by the Shannon diversity index, which is determined as follows:

[0032] H=-∑PilnPi

[0033] Where Pi is the ratio of the relative absorbance value of the ith well to the total relative absorbance value of the entire plate.

[0034] (3)Methods for measuring tobacco leaf output value and yield:

[0035] About 60 to 70 days after tobacco transplanting, the leaves begin to mature gradually from bottom to top. According to the maturity of the tobacco leaves, they are harvested in batches from bottom to top and placed in the drying room for drying. After baking, the yield is calculated; output value = average price × yield.

[0036] (4) Tobacco leaf sensory quality score:

[0037] The evaluation of the organoleptic quality is based on YC / T 138-1998. The evaluation indicators include aroma quality (10), aroma quantity (10), taste (12), miscellaneous smell (10), and irritation (10).

[0038] Table 1 Treatment of organic fertilizer combined with oxygenation test

[0039]

[0040]

[0041] In the test, strip application of organic fertilizer refers to strip application of fertilizer at the bottom of the tobacco planting row before ridge formation. For strip application of perforated aeration, a hole 8 cm deep is punched at one side of the ridge 10 cm away from the root of the tobacco plant using a flue-cured tobacco transplanting perforator. For strip application of 50% calcium peroxide for aeration, a hole 8 cm deep is punched at one side of the ridge 10 cm away from the root of the tobacco plant using a flue-cured tobacco transplanting perforator, and 40 g of 50% calcium peroxide is placed in the hole. For strip application of perforated aeration + 50% calcium peroxide for aeration, such as Figure 1 , On both sides of the ridge where the tobacco plants are planted, two holes with a depth of 8 cm are symmetrically made on the ridge platform 10 cm away from the roots of the tobacco plants. One hole is not treated as a hole for oxygenation, and 40 g of 50% calcium peroxide is placed in the other hole. ,

[0042] Organic fertilizer hole application refers to making a hole near the roots of tobacco seedlings after planting, putting organic fertilizer into the hole and covering it with a small amount of soil. For hole-application aeration, use a flue-cured tobacco transplanting puncher to make an 8 cm deep hole at one side of the ridge 10 cm away from the roots of the tobacco plants (on the side of the hole application location). For hole-application 50% calcium peroxide aeration, use a flue-cured tobacco transplanting puncher to make an 8 cm deep hole at one side of the ridge 10 cm away from the roots of the tobacco plants (on the side of the hole application location), and put 40g of 50% calcium peroxide into the hole. For hole-application aeration + 50% calcium peroxide aeration, on the ridge 10 cm away from the roots of the tobacco plants on one side of the ridge where the tobacco plants are planted, make two 8 cm deep holes on both sides of the organic fertilizer hole application location. One hole is not treated as a hole for aeration, and 40g of 50% calcium peroxide is put into the other hole. The deep holes are drilled by a puncher, which is a flue-cured tobacco transplanting puncher model RH-YZJ produced by Qufu Runhua Machinery Co., Ltd. The chemical oxygenator used is produced by Zhengzhou Huize Biochemical Technology Co., Ltd.

[0043] 1. Cow dung organic fertilizer

[0044] Judging from the field growth, whether it is applied in strips or holes, the growth of tobacco leaves under oxygenated conditions is significantly better than that of the control, and the field growth under the treatment of mixed oxygenation with punching + calcium peroxide is the best.

[0045] Cow dung organic fertilizer combined with different oxygenation measures has different degrees of improvement on tobacco yield, output value, quality, soil organic matter mineralization rate and soil microbial Shannon index, especially the aroma quality, aroma volume and aftertaste of the upper tobacco leaves have increased significantly, and the impurities have decreased significantly. Among them, the mixed oxygenation measures of punching + calcium peroxide have the best effect on improving the yield of tobacco leaves and the quality of middle tobacco leaves. It has a significant effect on increasing the aroma quality of middle tobacco leaves, with moderate strength and smoke concentration, reduced irritation and increased aftertaste. It can also significantly promote soil organic matter mineralization and soil microbial diversity. Punching and oxygenation have the most obvious effect on the quality of upper tobacco leaves. Overall, organic fertilizer combined with oxygenation has a significant effect on improving the middle tobacco leaves, and the upper tobacco leaves have improved.

[0046] Table 2 Evaluation of the field test effect of cow dung organic fertilizer combined with different oxygenation measures

[0047]

[0048] 2. Wine lees organic fertilizer

[0049] The trend of combined oxygenation of distiller's grains organic fertilizer and cow dung organic fertilizer was consistent. Regardless of strip application or hole application, the growth of tobacco leaves under oxygenated conditions was significantly better than that of the control. The field growth was best in the treatment of mixed oxygenation with aeration by drilling + calcium peroxide.

[0050] The combination of distiller's grains organic fertilizer and different oxygenation measures has improved tobacco yield, output value, quality, soil organic matter mineralization rate and soil microbial Shannon index to varying degrees. Among them, the mixed oxygenation measures of drilling + calcium peroxide have the best effect on improving tobacco yield, output value, middle tobacco leaves and upper tobacco leaves quality, and can significantly promote soil organic matter mineralization and soil microbial diversity.

[0051] Table 3 Evaluation of the field test effect of distiller's grains organic fertilizer combined with different oxygenation measures

[0052]

[0053]

[0054] The research method of the present invention is described in detail below:

[0055] 1. Determine the best oxygenation method for different organic fertilizers

[0056] For the types of organic fertilizers commonly used in flue-cured tobacco: cow dung organic fertilizer, oil cake organic fertilizer, distiller's grains organic fertilizer and biological organic fertilizer, safe and environmentally friendly oxygenation methods were selected: physical oxygenation (drilling oxygenation, pump oxygenation) and chemical oxygenation (calcium peroxide oxygenation, hydrogen peroxide oxygenation and sodium percarbonate oxygenation). The mineralization rate of soil organic matter and the agronomic traits of tobacco plants were evaluated for 100 days respectively, so as to determine the best oxygenation method for different organic fertilizers.

[0057] (1) Oxygenator packaging and pretreatment

[0058] The chemical oxygenator is wrapped in the blended gauze to achieve the purpose of slowly releasing the chemical oxygenator.

[0059] (2) Screening of oxygenation methods

[0060] Taking cow dung organic fertilizer as an example, the following five oxygenation methods are used (Table 4), which are mainly divided into physical oxygenation and chemical oxygenation. Physical oxygenation includes drilling and pumping oxygenation, and chemical oxygenation uses reagents such as calcium peroxide, hydrogen peroxide and sodium percarbonate.

[0061] Table 4 Cow dung organic fertilizer combined with oxygenation to promote the transformation of organic matter in the season

[0062]

[0063] 60 days after transplanting, Figure 4 The growth of tobacco plants treated with all oxygenation methods was better than that of the control. Among them, the agronomic traits of tobacco plants treated with hole aeration and calcium peroxide aeration were the best among all oxygenation methods.

[0064] Soil microbial activity varies under different oxygenation methods, see Figure 5Among them, the soil microbial diversity index and uniformity index under calcium peroxide aeration method were significantly higher than those under conventional fertilization and other aeration methods.

[0065] 2. Determine the optimal time / concentration of oxygenation

[0066] There is no problem of concentration setting for physical aeration by drilling holes. Once the holes are drilled, it is effective throughout the growth period. The time of pump aeration is set to: 0h / d, 4h / d, 8h / d and 12h / d. The concentrations of chemical aeration are set as shown in the following table.

[0067] Table 5 Chemical oxygenation concentration treatment

[0068]

[0069] Figure 6 The growth of cow dung organic fertilizer under different oxygenation times. Figure 7 The agronomic traits of cow dung organic fertilizer under different oxygenation times. As can be seen from the figure, under physical pump oxygenation, the oxygenation time of cow dung organic fertilizer is optimal at 12h / d, which is mainly reflected in promoting the stem girth and root growth of tobacco plants. Similarly, the oxygenation time of oil cake organic fertilizer is optimal at 8h / d and 12h / d. Considering the cost comprehensively, the recommended optimal time concentration of oil cake organic fertilizer is 8h / d. For wine lees organic fertilizer, the oxygenation time of 12h / d is optimal, which is mainly reflected in increasing the number of tobacco leaves and root growth. The effect of biological organic fertilizer oxygenation time of 8h / d is the best, and there is no significant difference in the above-ground and underground indicators compared with the 12h / d treatment. Some indicators such as leaf width are even better than 12h / d.

[0070] As shown in Table 6, under chemical oxygenation, from the perspective of agronomic traits, the agronomic traits of each oxygenating substance have a trend of change: medium concentration > low concentration > high concentration. In general, the agronomic traits of the medium concentration calcium peroxide treatment are significantly higher than those of other treatments. From the perspective of the changes in soil microbial abundance after oxygenation, except for the high concentration treatments of hydrogen peroxide and sodium percarbonate, the Shannon index of soil microorganisms in other treatments is significantly higher than that of the conventional control. Among them, the soil diversity index of the medium concentration of calcium peroxide and hydrogen peroxide is the highest and significantly higher than that of other treatments. In summary, the medium concentration of the three chemical oxygenators has the best effect.

[0071] Table 6 Agronomic traits and soil microbial abundance of different concentrations of cow dung organic fertilizer combined with chemical oxygenation

[0072] deal with Plant height / cm Stem circumference / cm Aboveground dry weight / g Root dry weight / g Soil Shannon Index CK 12.73b 9.37de 4.75def 0.71b 3.03d Ca1 17.03a 12.30a 7.58abc 1.36a 3.08c Ca2 19.03a 11.54ab 8.46a 1.80a 3.16a Ca3 16.30a 10.67bc 6.17cde 1.38a 3.05c H1 14.07b 8.92e 4.46f 0.48b 3.06c H2 16.30a 10.15cd 7.90a 1.65a 3.12a H3 13.91b 8.89e 6.25bcd 0.71b 3.04d Na1 14.73b 9.03e 5.37def 0.57b 3.02d Na2 16.31a 10.68bc 7.84ab 1.57a 3.10b Na3 13.59b 8.77e 4.57ef 0.86b 3.01d

[0073] 3. Determine the best oxygenation period

[0074] For organic fertilizer, oxygenation was carried out at 0d after transplanting, 20d after transplanting, 40d after transplanting and 60d after transplanting, and the oxygenation effect was evaluated to determine the optimal period of oxygenation.

[0075] Table 7 Optimal oxygenation time for cow dung organic fertilizer combined with pump oxygenation

[0076]

[0077] Figure 8 The best oxygenation time for cow dung organic fertilizer combined with pump oxygenation is the growth trend of tobacco. Fig. 9 , Fig.10 and Fig.11 This is a diagram of tobacco enzyme activity at the optimal oxygenation time for cow dung organic fertilizer combined with pump oxygenation. As can be seen from the figure, under the cow dung organic fertilizer combined with oxygenation measures, the acid phosphatase, sucrase, urease related to nutrient metabolism, the peroxidase and catalase related to stress resistance, and the polyphenol oxidase related to tobacco leaf quality in the soil have all been greatly improved compared with the non-oxygenated control, among which the level of improvement during the vigorous growth period is higher than the level of oxygenation during the transplanting period.

[0078] 4. Field trials to evaluate the effect of oxygenation

[0079] Physical oxygenation and chemical oxygenation were selected as one method, and the oxygenation effect was evaluated on relatively sticky tobacco-growing soils in four typical tobacco-growing areas, namely Bozhou District of Zunyi City, Guizhou Province, Dafang County of Bijie City, Xingren City of Qianxinan Prefecture, and Longgang Town of Guiyang City.

[0080] Taking into account the field yield, output value and rate of high-quality tobacco, the oxygenation method using calcium peroxide in the four regions had the best effect. Calcium peroxide oxygenation can significantly increase the yield, output value and rate of high-quality tobacco leaves.

[0081] Table 8 Field evaluation of cow dung organic fertilizer combined with oxygenation

[0082]

[0083]

[0084] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for promoting the seasonal conversion of tobacco organic matter by combining organic fertilizer with oxygenation, characterized in that: After the tobacco seedlings are transplanted on the ridge, two deep holes are opened on the ridge at a position 8-12 cm away from the roots of the tobacco seedlings, the bottom of the deep holes is in contact with the basal fertilizer on the ridge, calcium peroxide is put into one of the deep holes as a chemical oxygenation method, and the other deep hole is not treated as a physical oxygenation method, and the basal fertilizer is organic fertilizer; wherein, the deep holes are opened for oxygenation treatment during the vigorous growth period after the tobacco seedlings are transplanted; for the ridge body where organic fertilizer is applied as basal fertilizer in strips before transplanting, the two deep holes are symmetrically opened on the ridge surface on both sides of the tobacco seedlings; for the ridge body where organic fertilizer is applied as basal fertilizer in holes after transplanting, the two deep holes are opened on the ridge surface on one side of the tobacco seedlings, and are respectively located on both sides of the organic fertilizer hole application place; the calcium peroxide is 50% calcium peroxide; the depth of the two deep holes is 8 cm, and the holes are punched by a conventional transplanting puncher, and the amount of 50% calcium peroxide added is 35-45 g.

2. The method for promoting seasonal conversion of tobacco organic matter by combining organic fertilizer with oxygenation according to claim 1, characterized in that: The organic fertilizer includes cow dung organic fertilizer, oil cake organic fertilizer, distiller's grains organic fertilizer or biological organic fertilizer.

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