A device for preparing a polypeptide biological puffed compound feed

Abstract

The application provides a polypeptide biological puffed compound feed preparation device, which comprises a stirring bin, a first discharge port is arranged at the lower end of the stirring bin, the first discharge port is communicated with the lower part of an intermediate partition plate, the side wall of the stirring bin is made of water permeable material, a liquid outlet is arranged on the side wall of a tank body and close to the upper part of the intermediate partition plate, a first motor is arranged at the middle part of the upper end of the tank body, an output end of the first motor is connected with a stirring rod and a plurality of telescopic rods, and a pressing plate is fixed to the lower end of each telescopic rod. The device solves the problems of poor coordination between existing feed processing equipment, the need to transport materials between different equipment, and time and labor.

Description

[0001] This invention is a divisional application of the invention patent with application number "202210913310.7", entitled "A preparation device and preparation method for polypeptide bio-expanded compound feed". Technical Field

[0002] This invention belongs to the technical field of feed processing equipment, and specifically relates to a device for preparing polypeptide bio-expanded compound feed. Background Technology

[0003] Shrimp are among the highest-yielding and most economically valuable marine shrimp species. In recent years, commercial shrimp farming has been widespread, and production is rapidly increasing. Formulated feed is the material basis for healthy shrimp farming.

[0004] Feed processing is a crucial stage in feed production. Existing feed processing equipment mainly includes unloading, storage, crushing, mixing, and packaging equipment. However, the coordination between these devices is poor, requiring material transfer between different stages. This is especially problematic when extracting liquids from the feed mixture; the mixture must be transported to dedicated extrusion or separation equipment to extract the liquid before being moved to the next stage, which is time-consuming and labor-intensive. For example, Chinese patent CN201520281720.X provides a pneumatic feed processing unit, including a mixer, a pulverizer, a rotary feed valve, a Roots blower, a dust filter, a noise reducer, and a material filter. The pulverizer is connected above the mixer, and the pulverizer is connected to the feed hopper. The rotary feed valve is connected below the mixer. The material filter on the mixer is connected to an air volume regulator. One end of the air volume regulator is connected to the material hopper via the rotary feed valve, and the other end is connected to the Roots blower via the dust filter and noise reducer. This processing unit utilizes a large amount of piping and transportation equipment, resulting in significant time and labor costs. Summary of the Invention

[0005] The purpose of this invention is to solve the problem of poor coordination between existing feed processing equipment, requiring material transfer between different devices, especially when extracting liquid from feed mixtures. This necessitates transporting the feed mixture to dedicated extrusion or separation equipment to extract the liquid before proceeding to the next stage, which is time-consuming and labor-intensive. To solve the above technical problems, this invention discloses the following technical solution:

[0006] A preparation apparatus for polypeptide bio-expanded compound feed includes a tank and an extrusion conveyor, wherein the tank comprises:

[0007] A first feeding port is located at the upper end of the tank; the first feeding port is provided with...

[0008] A feeding gate, which can control the opening and closing of the first feeding port;

[0009] A middle partition is fixed to the middle part of the tank body;

[0010] A stirring chamber is disposed above the intermediate partition; the lower end of the stirring chamber has an opening.

[0011] The first discharge port connects the agitation chamber to the lower part of the intermediate partition.

[0012] A discharge valve is provided at the first discharge port;

[0013] The grinding chamber is located below the discharge port and is rotatably connected to the side wall of the tank.

[0014] The second motor, the output end of which is fixedly connected to the grinding chamber;

[0015] The second discharge port is located at the bottom of the tank.

[0016] The lower part of the second discharge port is connected to the inlet of the puffing conveyor;

[0017] The side wall of the stirring chamber is made of a water-permeable material. There is a gap between the side wall of the stirring chamber and the side wall of the tank. An outlet is provided on the side wall of the tank, close to the middle partition. An outlet valve is provided on the outlet.

[0018] A first motor is installed in the middle of the upper end of the tank. The output end of the first motor is connected to a stirring rod and multiple telescopic rods. A pressure plate is fixed to the lower end of each telescopic rod.

[0019] Furthermore, the preparation device also includes a blower, the inlet of which is connected to the upper part of the end of the puffing conveyor, and the outlet is connected to the side wall of the tank between the stirring chamber and the grinding chamber and the second discharge port.

[0020] Furthermore, an electric heater is provided at the output end of the blower.

[0021] Furthermore, there are two first feeding ports, located at the two ends of the top of the tank body respectively; the feeding gate includes a circular sliding ring, and the top of the tank body is provided with a groove that cooperates with the sliding ring, and the sliding ring slides in contact with the groove; two baffle plates are fixedly connected to the two ends of the sliding ring respectively.

[0022] Furthermore, a grinding disc is provided inside the grinding chamber, and a gravity sensor is provided below the grinding disc. When the gravity sensor detects that the weight has reached a set value, the first discharge port is closed.

[0023] Furthermore, a second feeding port is provided between the second discharge port and the puffing conveyor.

[0024] A method for preparing polypeptide bio-expanded compound feed using the aforementioned apparatus includes the following steps:

[0025] Open the feeding door and add a measured amount of fermentation raw materials and liquid inoculum into the stirring chamber through the first feeding port, then close the feeding door;

[0026] Turn on the first motor to drive the stirring rod to stir and mix the materials in the stirring chamber for fermentation; after fermentation is complete, open the feeding door, add enzymes and water, then close the feeding door, start the first motor again to stir and carry out enzymatic hydrolysis; after enzymatic hydrolysis is completed, open the liquid outlet valve, start each telescopic rod, and push each pressure plate to squeeze the materials in the stirring chamber from top to bottom, so that some liquid passes through the side wall of the stirring chamber and flows out from the liquid outlet;

[0027] Open the feeding door and add the ingredients for the first batching mixture; close the feeding door and start the first motor to stir and mix; open the discharge valve, and part of the polypeptide mixture falls from the stirring chamber into the grinding chamber; close the discharge valve, and the grinding chamber performs ultra-fine pulverization on the material inside; after pulverization is completed, start the second motor to drive the grinding chamber to rotate, and the ground material falls to the bottom of the tank; start the puffing conveyor to continuously carry away the material from the bottom of the tank, while simultaneously adding the liquid ingredients for the second batching mixture from the second feeding port, and the puffing conveyor outputs puffed granules.

[0028] Furthermore, the liquid bacterial strain consists of: Bifidobacterium 10⁸ CFU / g, Lactobacillus dryis 2×10⁸ CFU / g, Bacillus subtilis 10⁸ CFU / g, and Bacillus licheniformis 2×10⁸ CFU / g.

[0029] Furthermore, the secondary batching and mixing includes adding 20-60 kg of phospholipid oil, 40-80 kg of fish oil, 1-2 kg of bile acids, 0.2-0.3 kg of glutathione, and 30-50 kg of vitamins to each ton of the primary batching mixture after ultrafine grinding.

[0030] Beneficial effects:

[0031] 1. The preparation device of the present invention integrates mixing, fermentation, enzymatic hydrolysis, liquid extraction, pulverization, and puffing granulation into one unit. It can perform multiple operations simultaneously, and the multiple operations are interconnected, reducing the handling of materials between different equipment. It has a high degree of automation and saves manpower.

[0032] 2. This invention can automatically extract liquid from feed mixtures without the need for material handling, saving time and labor, and ensuring thorough extraction. It can also control the extraction volume, thereby controlling the moisture content of the mixture after liquid extraction.

[0033] 3. The preparation device of the present invention utilizes the excess heat of the puffing conveyor to provide heat for fermentation, enzymatic hydrolysis and preheating before puffing, thereby improving energy efficiency.

[0034] 4. By combining four common fermentation strains and comparing them experimentally, the optimal strain ratio was selected, enabling more proteins to be fermented and converted into peptides.

[0035] 5. The secondary ingredient mixing is set after the ultrafine grinding to prevent the ultrafine grinding from damaging unstable components. Attached Figure Description

[0036] Figure 1 This is a structural diagram of a polypeptide bio-expanded compound feed preparation device according to an embodiment of the present invention;

[0037] Figure 2 This is a schematic diagram of the internal structure of the tank in an embodiment of the present invention;

[0038] Figure 3 This is a structural diagram of the stirring rod, telescopic rod, and pressure plate according to an embodiment of the present invention;

[0039] Figure 4 This is a structural diagram of the heating system according to an embodiment of the present invention;

[0040] Figure 5 This is a structural diagram of the feeding gate in an embodiment of the present invention.

[0041] In the diagram: 1-Tank body, 2-First feeding port, 3-Feeding door, 301-Sliding ring, 302-Baffle plate, 4-Intermediate partition plate, 5-Agitating chamber, 6-First discharge port, 7-Discharge valve, 8-Grinding chamber, 9-Second motor, 10-Second discharge port, 11-Expanding conveyor, 12-Blower, 13-Electric heater, 14-Liquid outlet, 15-Liquid valve, 16-First motor, 17-Agitating rod, 18-Telescopic rod, 19-Pressure plate, 20-Grinding disc, 21-Second feeding port, 22-Three-way regulating valve. Detailed Implementation

[0042] The present invention will now be described in detail and clearly with reference to the embodiments.

[0043] Example 1

[0044] This embodiment provides a polypeptide bio-expanded compound feed preparation device, including a tank 1. A first feeding port 2 is provided at the upper end of the tank 1, and a feeding gate 3 is provided on the first feeding port 2. Exemplarily, there are two first feeding ports 2, located at opposite ends of the top of the tank 1. The feeding gate 3 includes a circular sliding ring 301, and a groove is provided on the top of the tank 1 to mate with the sliding ring 301, allowing the sliding ring 301 to slide in contact with the groove. Two baffle plates 302 are fixedly connected to the sliding ring 301. By rotating the sliding ring 301, the two baffle plates 302 can simultaneously cover or simultaneously open the first feeding port 2. A middle partition 4 is provided in the middle of the tank 1, and an agitation chamber 5 is located above the middle partition 4. A temperature sensor is installed inside the agitation chamber 5. The sidewalls of the agitation chamber 5 are made of a permeable material; exemplarily, permeable concrete can be used. A gap is left between the side wall of the stirring chamber 5 and the side wall of the tank body 1. A liquid outlet 14 is opened on the side wall of the tank body 1, close to the upper part of the intermediate partition 4, and a liquid outlet valve 15 is installed on the liquid outlet 14. A first motor 16 is installed in the middle of the upper end of the tank body 1. The output end of the first motor 16 is connected to a stirring rod 17 for stirring the material in the stirring chamber 5. The output end of the first motor 16 is also connected to multiple telescopic rods 18, and a pressure plate 19 is fixed to the lower end of each telescopic rod 18. A first discharge port 6 is opened at the lower end of the stirring chamber 5, and a discharge valve 7 is installed on the first discharge port 6. A grinding chamber 8 is installed below the stirring chamber 5. The grinding chamber 8 is rotatably connected to the side wall of the tank body 1. A second motor 9 is fixed to the side wall of the tank body 1. The output end of the second motor 9 is fixedly connected to the grinding chamber 8 and can control the grinding chamber 8 to rotate. A grinding disc 20 is installed inside the grinding chamber 8. A gravity sensor is installed below the grinding disc 20 to detect the magnitude of the gravity borne by the grinding disc 20. The grinding disc 20 is existing technology and will not be described in detail here. Furthermore, the side wall of the grinding chamber 8 is folded inward at the top to prevent material from flying out during the grinding process. The bottom of the tank body 1 has a conical structure, and a second discharge port 10 is opened at the center of the conical structure.

[0045] The lower part of the second discharge port 10 is connected to the inlet of the puffing conveyor 11. The puffing conveyor 11 is prior art and will not be described in detail here. A second feeding port 21 is also provided between the second discharge port 10 and the puffing conveyor 11. The second feeding port 21 is used to convey liquid materials, and the feeding amount is controlled by a flow controller, which is prior art.

[0046] The feed preparation device is also equipped with a heating system, including a blower 12. The inlet of the blower 12 is connected to the upper part of the end of the extrusion conveyor 11, and the outlet is connected to the side wall of the tank 1 between the stirring chamber 5 and the grinding chamber 8 and the second discharge port 10. The blower 12 extracts the excess heat generated by the extrusion conveyor 11 and transports it into the tank 1. An electric heater 13 is installed at the output end of the blower 12 to supplement the heat. A three-way regulating valve 22 is installed at the rear end of the electric heater 13 to regulate the airflow of the two heating pipes entering the tank 1.

[0047] Example 2

[0048] This embodiment provides a method for preparing polypeptide bio-expanded compound feed using the apparatus in Example 1, including the following steps:

[0049] Open the feeding door 3 and add a measured amount of fermentation raw materials and liquid inoculum into the stirring chamber 5 through the first feeding port 2, then close the feeding door 3. Preferably, the liquid inoculum consists of: Bifidobacterium 10⁸ CFU / g, Lactobacillus dryis 2×10⁸ CFU / g, Bacillus subtilis 10⁸ CFU / g, and Bacillus licheniformis 2×10⁸ CFU / g.

[0050] The first motor 16 is turned on, driving the stirring rod 17 to agitate and mix the materials in the stirring chamber 5. A temperature sensor detects the temperature inside the stirring chamber 5 and controls the blower 12, electric heater 13, and three-way regulating valve 22 to provide heat to the stirring chamber 5 and maintain the fermentation temperature. After fermentation is complete, the feeding door 3 is opened, enzymes and water are added, and then the feeding door 3 is closed. The first motor 16 is started again to agitate, and the temperature inside the stirring chamber 5 is controlled in the same way for enzymatic hydrolysis. After enzymatic hydrolysis, the liquid outlet valve 15 is opened, and each telescopic rod 18 is activated, pushing each pressure plate 19 to squeeze the materials inside the stirring chamber 5 from top to bottom, allowing most of the liquid to pass through the side wall of the stirring chamber 5 and flow out from the liquid outlet 14. To extract as much polypeptide liquid as possible, after one squeezing, the pressure plate 19 can be lifted upwards, the stirring rod 17 can be used to agitate the materials appropriately, and then squeezing can be performed again to extract the polypeptide liquid. By controlling the squeezing force, the amount of liquid extracted can be controlled, thereby controlling the moisture content in the mixture after liquid extraction. The remaining polypeptide mixture with low water content in agitation chamber 5 is used to prepare shrimp feed.

[0051] Open the feeding door 3 and add the ingredients for the first batching. Preferably, the ingredients for the first batching include 250-400 kg of astaxanthin per ton of mixture. Close the feeding door 3 and start the first motor 16 to stir and mix. Open the discharge valve 7, and part of the peptide mixture falls from the stirring chamber 5 into the grinding chamber 8. When the gravity sensor in the grinding chamber 8 detects that the set value has been reached, close the discharge valve 7 to control the weight of the ground material. Start the grinding disc 20 to perform ultrafine grinding of the material. After grinding is completed, start the second motor 9 to rotate the grinding chamber 8 180 degrees, and the ground material falls to the bottom of the tank 1. Start the puffing conveyor 11 to continuously remove the material from the bottom of the tank 1, while simultaneously adding the liquid ingredients for the second batching through the second feeding port 21. Preferably, the secondary batching mixture includes: 20-60 kg of phospholipid oil, 40-80 kg of fish oil, 1-2 kg of bile acids, 0.2-0.3 kg of glutathione, and 30-50 kg of vitamins added to each ton of the primary batching mixture after ultrafine grinding. After the extruded granules are output from the extrusion conveyor 11, they are then dried and cooled. Furthermore, vacuum spraying and constant-temperature anaerobic fermentation technology can be used to anaerobic ferment the dried extruded feed to kill molds and harmful bacteria.

[0052] In this embodiment, the stirring chamber 5, grinding chamber 8, and puffing conveyor 11 of the feed preparation device can operate simultaneously. The blower 12 extracts excess heat from the end of the puffing conveyor 11 and transports it to the stirring chamber 5 to provide heat for fermentation and enzymatic hydrolysis. At the same time, it can transport heat to the bottom area of ​​the tank 1 to preheat the material to be puffed, thereby reducing energy consumption.

[0053] Example 3

[0054] This embodiment provides detailed preparation steps and specific preparation conditions for polypeptide bio-expanded compound feed:

[0055] 1. Raw material screening and proportioning: Crush the fish and shrimp raw materials to 40 mesh and filter to obtain fish and shrimp paste; take 20-40 parts of fish and shrimp paste and 60-80 parts of peanut meal, mix and stir evenly to form a raw material mixture.

[0056] 2. Microbial fermentation:

[0057] (1) Screening and identification of functional lactic acid bacteria

[0058] After research, four of the most commonly used feed fermentation bacteria strains were selected, including: Bifidobacterium, Lactobacillus simethicone, Bacillus subtilis, and Bacillus licheniformis.

[0059] (2) Comparison Experiment of Microbial Strains and Formulas

[0060] Each group used the same raw material composition (30 parts fish and shrimp paste, 70 parts peanut meal) and the same total mass of microbial strains. The fermentation experiments were conducted using the 11 microbial strain ratios shown in Table 1 (Bifidobacterium: Lactobacillus dry form: Bacillus subtilis: Bacillus licheniformis) and the same operating methods. After reaching the fermentation endpoint, the following indicators were measured: moisture, small peptides, pH, and crude protein. Regarding fermentation time and endpoint: the fermentation completion time was controlled between 72-96 hours depending on the ambient temperature. The optimal fermentation temperature was 34℃-39℃. The criteria for successful fermentation were: a fermented, slightly acidic aroma and a pH value around 5. Based on the evaluation of small peptide content and pH, strain #10 (Bifidobacterium: Lactobacillus dry form: Bacillus subtilis: Bacillus licheniformis = 1:2:1:2) showed advantages and was selected as the main microbial strain formula for subsequent bio-fermented extruded feed.

[0061]

[0062]

[0063] Table 1 Evaluation of the fermentation effect of different fermentation inoculants on bio-fermented extruded feed

[0064] (3) Preparation of liquid culture and fermentation

[0065] Content of each bacterial species: Bifidobacterium 10⁸ CFU / g, Lactobacillus dry acid 2×10⁸ CFU / g, Bacillus subtilis 10⁸ CFU / g, Bacillus licheniformis 2×10⁸ CFU / g.

[0066] The raw material mixture and liquid inoculum are mixed at a ratio of 1:0.3 to form a microbial-raw material mixture, which is then fermented at a temperature of 34-39℃ for 72-96 hours to obtain the fermented product.

[0067] 3. Enzymatic hydrolysis: Add 1 kg of exogenous alkaline protease and 0.4 tons of water to every 1 ton of fermented mixture, mix well, and hydrolyze at 50-60℃ for 8-12 hours.

[0068] 4. Extraction of polypeptide solution: The enzymatically hydrolyzed mixture is dehydrated to obtain a polypeptide mixture with low water content, as well as a polypeptide solution.

[0069] 5. Primary ingredient mixing: Add 250-400 kg of astaxanthin to each ton of dehydrated polypeptide mixture to obtain the primary ingredient mixture.

[0070] 6. Ultrafine grinding: Ultrafine grinding is used to pulverize the mixture of ingredients in a single batch.

[0071] 7. Secondary ingredient mixing: Add 20-60 kg of phospholipid oil, 40-80 kg of fish oil, 1-2 kg of bile acids, 0.2-0.3 kg of glutathione, and 30-50 kg of vitamins to each ton of the primary ingredient mixture after ultrafine grinding, and mix to obtain the secondary ingredient mixture; the secondary ingredient mixing is set after ultrafine grinding to prevent ultrafine grinding from damaging unstable components.

[0072] 8. Extrusion and granulation: The secondary batching mixture is put into an extruder for extrusion and granulation to obtain extruded granules.

[0073] 9. Drying and Cooling: After the extruded granules are dried in the dryer, the moisture content is controlled to be <10%, and the drying temperature is controlled between 50-60℃ to avoid damage to easily pyrolytic components such as vitamins; then they are cooled by a cooler.

[0074] 10. Vacuum Spraying: Vacuum spraying technology is used to uniformly spray active substances such as the bacterial-enzyme composite preparation liquid onto the surface of the expanded granules and penetrate into the interior of the granules. The bacterial-enzyme composite preparation includes a composite enzyme preparation, a fermentation culture medium, and a composite bacterial preparation. The compound microbial preparation comprises one or more of the following: *Lactobacillus plantarum*, *Enterococcus faecalis*, *Saccharomyces cerevisiae*, *Bacillus subtilis*, *Bacillus licheniformis*, *Bacillus megaterium*, *Bacillus coagulans*, and *Clostridium butyricum*. The fermentation medium comprises the following components in parts by weight: molasses 10-20 parts, corn steep liquor powder 3-5 parts, glucose 6-10 parts, sodium chloride 0.3-0.5 parts, potassium dihydrogen phosphate 0.2-0.25 parts, ammonium dihydrogen phosphate 0.2-0.25 parts, magnesium sulfate 0.15-0.2 parts, ferrous sulfate 0.004-0.005 parts, Tween 80 0.5-1 parts, and water 800-1000 parts. The compound enzyme preparation comprises one or more of the following: phytase, neutral protease, amylase, pectinase, galactosidase, cellulase, glucanase, lipase, maltase, and mannanase.

[0075] 11. Constant Temperature Anaerobic Fermentation: Under sealed conditions, maintaining a temperature between 25-40℃, fermentation for 10-15 days yields fermented soft pellet feed. Numerous experiments have demonstrated that during anaerobic fermentation, molds (aerobic bacteria) are completely killed. Meanwhile, bacteria in the feed ingredients, such as E. coli and Salmonella, which can survive under anaerobic conditions, work in conjunction with Bifidobacterium, Lactobacillus dry acidophilus, Bacillus subtilis, and Bacillus licheniformis, rapidly consuming oxygen and creating an acidic environment. Under anaerobic and acidic conditions, the growth and reproduction of harmful bacteria are inhibited until they are killed, thus blocking the risk of pathogenic bacteria from entering the feed. This allows for a complete replacement of antibiotics, achieving antibiotic-free feeding.

[0076] 12. Screening, grading, and packaging to form finished feed.

[0077] Example 4

[0078] This embodiment provides a preparation process for polypeptide bio-expanded compound feed.

[0079] Raw material screening and proportioning: Crush the fish and shrimp raw materials to 40 mesh and filter to obtain fish and shrimp paste; take 20 parts of fish and shrimp paste and 80 parts of peanut meal, mix and stir evenly to form a raw material mixture.

[0080] Microbial fermentation: A liquid inoculum was prepared, containing: Bifidobacterium 10⁸ CFU / g, Lactobacillus dryis 2 × 10⁸ CFU / g, Bacillus subtilis 10⁸ CFU / g, and Bacillus licheniformis 2 × 10⁸ CFU / g. The raw material mixture and the liquid inoculum were mixed at a ratio of 1:0.3 to form a microbial-raw material mixture, which was fermented at 36℃ for 82 hours to obtain the fermented product. The fermentation product contained the following indicators: moisture 41.63%, small peptides 10.21%, pH 5.10, and crude protein 26.26%.

[0081] Enzymatic hydrolysis: Add 1 kg of exogenous alkaline protease and 0.4 tons of water to every 1 ton of fermented mixture, mix well, and hydrolyze at 50°C for 12 hours.

[0082] Extracting peptide solution: The enzymatically hydrolyzed mixture is dehydrated to obtain a peptide mixture with low water content, as well as a peptide solution.

[0083] Primary ingredient mixing: Add 250 kg of astaxanthin to each ton of peptide mixture to obtain the primary ingredient mixture.

[0084] Ultrafine grinding: Ultrafine grinding is used to pulverize a mixture of ingredients in a single batch.

[0085] Secondary ingredient mixing: Add 40 kg of phospholipid oil, 60 kg of fish oil, 1 kg of bile acid, 0.2 kg of glutathione, and 40 kg of vitamins to each ton of the primary ingredient mixture after ultrafine grinding, mix, and obtain the secondary ingredient mixture.

[0086] Extrusion granulation: The secondary batching mixture is put into an extruder for extrusion granulation to obtain extruded granules.

[0087] Drying and cooling: After the extruded granules are dried in the dryer and the moisture content is controlled to be less than 10%, they are then cooled by a cooler.

[0088] Vacuum spraying: Using vacuum spraying technology, the liquid of the bacterial-enzyme composite preparation in Example 3 is uniformly sprayed onto the surface of the expanded granules and penetrates into the interior of the granules.

[0089] Constant temperature anaerobic fermentation: Under sealed conditions, the temperature is maintained at 30℃, and fermentation is carried out for 15 days to obtain fermented soft pellet feed.

[0090] The feed is then screened, graded, and packaged to produce finished products.

[0091] Example 5

[0092] This embodiment provides a preparation process for polypeptide bio-expanded compound feed.

[0093] Raw material screening and proportioning: Crush the fish and shrimp raw materials to 40 mesh and filter to obtain fish and shrimp paste; take 40 parts of fish and shrimp paste and 60 parts of peanut meal, mix and stir evenly to form a raw material mixture.

[0094] Microbial fermentation: A liquid inoculum was prepared, containing: Bifidobacterium 10⁸ CFU / g, Lactobacillus dryis 2 × 10⁸ CFU / g, Bacillus subtilis 10⁸ CFU / g, and Bacillus licheniformis 2 × 10⁸ CFU / g. The raw material mixture and the liquid inoculum were mixed at a ratio of 1:0.3 to form a microbial-raw material mixture, which was fermented at 39℃ for 96 hours to obtain the fermented product. The fermentation product contained the following indicators: moisture 41.78%, small peptides 10.42%, pH 5.03, and crude protein 26.07%.

[0095] Enzymatic hydrolysis: Add 1 kg of exogenous alkaline protease and 0.4 tons of water to every 1 ton of fermented mixture, mix well, and hydrolyze at 60°C for 8 hours.

[0096] Extracting peptide solution: The enzymatically hydrolyzed mixture is dehydrated to obtain a peptide mixture with low water content, as well as a peptide solution.

[0097] Primary ingredient mixing: Add 400 kg of astaxanthin to each ton of peptide mixture to obtain the primary ingredient mixture.

[0098] Ultrafine grinding: Ultrafine grinding is used to pulverize a mixture of ingredients in a single batch.

[0099] Secondary ingredient mixing: Add 60 kg of phospholipid oil, 80 kg of fish oil, 2 kg of bile acid, 0.3 kg of glutathione, and 50 kg of vitamins to each ton of the primary ingredient mixture after ultrafine grinding, and mix to obtain the secondary ingredient mixture.

[0100] Extrusion granulation: The secondary batching mixture is put into an extruder for extrusion granulation to obtain extruded granules.

[0101] Drying and cooling: After the extruded granules are dried in the dryer and the moisture content is controlled to be less than 10%, they are then cooled by a cooler.

[0102] Vacuum spraying: Using vacuum spraying technology, the liquid of the bacterial-enzyme composite preparation in Example 3 is uniformly sprayed onto the surface of the expanded granules and penetrates into the interior of the granules.

[0103] Constant temperature anaerobic fermentation: Under sealed conditions, the temperature is maintained at 25℃, and fermented soft pellet feed is obtained after 13 days of fermentation.

[0104] The feed is then screened, graded, and packaged to produce finished products.

[0105] Example 6

[0106] This embodiment provides a preparation process for polypeptide bio-expanded compound feed.

[0107] Raw material screening and proportioning: Crush the fish and shrimp raw materials to 40 mesh and filter to obtain fish and shrimp paste; take 30 parts of fish and shrimp paste and 70 parts of peanut meal, mix and stir evenly to form a raw material mixture.

[0108] Microbial fermentation: A liquid inoculum was prepared, containing: Bifidobacterium 10⁸ CFU / g, Lactobacillus dryis 2 × 10⁸ CFU / g, Bacillus subtilis 10⁸ CFU / g, and Bacillus licheniformis 2 × 10⁸ CFU / g. The raw material mixture and the liquid inoculum were mixed at a ratio of 1:0.3 to form a microbial-raw material mixture, which was fermented at 34℃ for 84 hours to obtain the fermented product. The fermentation product contained the following indicators: moisture 41.72%, small peptides 10.36%, pH 5.03, and crude protein 26.13%.

[0109] Enzymatic hydrolysis: Add 1 kg of exogenous alkaline protease and 0.4 tons of water to every 1 ton of fermented mixture, mix well, and hydrolyze at 55°C for 10 hours.

[0110] Extracting peptide solution: The enzymatically hydrolyzed mixture is dehydrated to obtain a peptide mixture with low water content, as well as a peptide solution.

[0111] Primary ingredient mixing: Add 350 kg of astaxanthin to each ton of peptide mixture to obtain primary ingredient mixture.

[0112] Ultrafine grinding: Ultrafine grinding is used to pulverize a mixture of ingredients in a single batch.

[0113] Secondary ingredient mixing: Add 20 kg of phospholipid oil, 40 kg of fish oil, 2 kg of bile acid, 0.3 kg of glutathione, and 40 kg of vitamins to each ton of the primary ingredient mixture after ultrafine grinding, and mix to obtain the secondary ingredient mixture.

[0114] Extrusion granulation: The secondary batching mixture is put into an extruder for extrusion granulation to obtain extruded granules.

[0115] Drying and cooling: After the extruded granules are dried in the dryer and the moisture content is controlled to be less than 10%, they are then cooled by a cooler.

[0116] Vacuum spraying: Using vacuum spraying technology, the liquid of the bacterial-enzyme composite preparation in Example 3 is uniformly sprayed onto the surface of the expanded granules and penetrates into the interior of the granules.

[0117] Constant temperature anaerobic fermentation: Under sealed conditions, the temperature is maintained at 40℃, and fermented soft pellet feed is obtained after 10 days of fermentation.

[0118] The feed is then screened, graded, and packaged to produce finished products.

[0119] Example 7

[0120] This embodiment provides a preparation process for polypeptide bio-expanded compound feed.

[0121] Raw material screening and proportioning: Crush the fish and shrimp raw materials to 40 mesh and filter to obtain fish and shrimp paste; take 30 parts of fish and shrimp paste and 70 parts of peanut meal, mix and stir evenly to form a raw material mixture.

[0122] Microbial fermentation: A liquid inoculum was prepared, containing: Bifidobacterium 10⁸ CFU / g, Lactobacillus dryis 2 × 10⁸ CFU / g, Bacillus subtilis 10⁸ CFU / g, and Bacillus licheniformis 2 × 10⁸ CFU / g. The raw material mixture and the liquid inoculum were mixed at a ratio of 1:0.3 to form a microbial-raw material mixture, which was fermented at 37℃ for 72 hours to obtain the fermented product. The fermentation product contained the following indicators: moisture 41.75%, small peptides 10.44%, pH 5.04, and crude protein 26.02%.

[0123] Enzymatic hydrolysis: Add 1 kg of exogenous alkaline protease and 0.4 tons of water to every 1 ton of fermented mixture, mix well, and hydrolyze at 55°C for 9 hours.

[0124] Extracting peptide solution: The enzymatically hydrolyzed mixture is dehydrated to obtain a peptide mixture with low water content, as well as a peptide solution.

[0125] Primary ingredient mixing: Add 350 kg of astaxanthin to each ton of peptide mixture to obtain primary ingredient mixture.

[0126] Ultrafine grinding: Ultrafine grinding is used to pulverize a mixture of ingredients in a single batch.

[0127] Secondary ingredient mixing: Add 40 kg of phospholipid oil, 60 kg of fish oil, 2 kg of bile acid, 0.3 kg of glutathione, and 40 kg of vitamins to each ton of the primary ingredient mixture after ultrafine grinding, mix, and obtain the secondary ingredient mixture.

[0128] Extrusion granulation: The secondary batching mixture is put into an extruder for extrusion granulation to obtain extruded granules.

[0129] Drying and cooling: After the extruded granules are dried in the dryer and the moisture content is controlled to be less than 10%, they are then cooled by a cooler.

[0130] Vacuum spraying: Using vacuum spraying technology, the liquid of the bacterial-enzyme composite preparation in Example 3 is uniformly sprayed onto the surface of the expanded granules and penetrates into the interior of the granules.

[0131] Constant temperature anaerobic fermentation: Under sealed conditions, the temperature is maintained at 35℃, and fermented soft pellet feed is obtained after 13 days of fermentation.

[0132] The feed is then screened, graded, and packaged to produce finished products.

[0133] Results Survey:

[0134] Survey 1:

[0135] To investigate the effect of the bio-fermented extruded feed of this invention as a substitute for feed in factory-farmed Litopenaeus vannamei, this study conducted a growth comparison experiment between it and ordinary commercial feed. Three experimental groups (A, B, and C) were set up at a factory-farmed shrimp farming base. Group A used low-protein commercial feed, Group B used the bio-fermented extruded feed of this invention, and Group C used high-protein commercial feed. Nine farming ponds were randomly divided into three groups, with three replicates per group. Each pond was stocked with 8000-10000 healthy Litopenaeus vannamei. During the experimental period, the shrimp were fed four times a day at 06:00, 10:00, 16:00, and 20:00, at a rate of 4% of their body weight. Water quality parameters such as feed amount, water temperature, pH, salinity, and dissolved oxygen were recorded. Feeding platforms were placed in the ponds, and the shrimp's feeding behavior was observed one hour after feeding. Twenty Litopenaeus vannamei shrimp were randomly collected from each experimental pond at the factory-scale shrimp farming base twice, with a 30-day interval. Their body length and weight were measured, and the average values ​​were calculated. At the end of the shrimp farming season, the total yield of each pond at the factory-scale shrimp farming base was tallied.

[0136] The experimental results showed no significant differences between group B (using the bio-fermented extruded feed of this invention) and group C (high-protein feed) in terms of final body weight, weight gain rate, specific growth rate, body composition, and TOR gene expression level in Litopenaeus vannamei. Group B significantly outperformed group A (low-protein feed) in all growth indicators, while group C's indicators fell between groups A and B. The bio-fermented extruded feed group exhibited similar growth effects to the high-protein feed group, but significantly better than the low-protein feed group. Therefore, bio-fermented extruded feed can replace the high-protein feed for the entire Litopenaeus vannamei farming process. Field technicians reported that after consistently feeding bio-fermented extruded feed for 2-3 weeks, shrimp showed increased appetite, significantly higher feed intake, significantly faster growth rate, significantly enhanced vitality, brighter shrimp bodies, firmer flesh, and clearer hepatopancreatic edges. Practice has proven that feeding bio-fermented extruded feed throughout the entire farming process, combined with water quality control techniques advocated in the ecological shrimp farming model, resulted in significantly stronger shrimp bodies and a marked improvement in shrimp immunity, stress resistance, and anti-stress ability.

[0137] Survey 2:

[0138] To investigate the effect of the bio-fermented extruded feed of this invention as a substitute for factory-farmed Litopenaeus vannamei feed, a comparative farming experiment was conducted at a shrimp demonstration base. After two years of production comparison, the experimental group (using the bio-fermented extruded feed of this invention) had an average shrimp weight of 17.1g and an average body length of 11.8cm, while the control group (using a commercially available high-protein feed) had an average shrimp weight of 15.2g and an average body length of 11.1cm. The yield per unit area of ​​the experimental group was 5.69kg / m², while that of the control group was 5.12kg / m², representing an 11.13% increase in yield compared to the control group.

[0139] Obviously, the specific implementation of this invention is not limited to the above-described manner. Any non-substantial improvements made using the inventive concept and technical solution of this invention, or the direct application of the inventive concept and technical solution to other situations without modification, are all within the scope of protection of this invention.

Claims

1. A preparation apparatus for polypeptide bio-expanded compound feed, comprising a tank (1) and an extrusion conveyor (11), characterized in that, The tank (1) includes: a first feeding port (2) located at the upper end of the tank (1); a feeding gate (3) provided on the first feeding port (2), the feeding gate (3) being able to control the opening and closing of the first feeding port (2); a middle partition (4) fixed in the middle of the tank (1); a stirring chamber (5) located above the middle partition (4); a first discharge port (6) opened at the lower end of the stirring chamber (5), the first discharge port (6) connecting the stirring chamber (5) to the lower part of the middle partition (4); and a discharge valve (7) located at the first... The first discharge port (6) is located below the first discharge port (6) and is rotatably connected to the side wall of the tank (1). The second motor (9) is fixedly connected to the grinding chamber (8). The second discharge port (10) is located at the bottom of the tank (1). The lower part of the second discharge port (10) is connected to the inlet of the puffing conveyor (11). The side wall of the stirring chamber (5) is made of permeable material. A gap is left between the side wall of the stirring chamber (5) and the side wall of the tank (1), which is close to the upper part of the middle partition (4). A liquid outlet (14) is provided on the side wall of the tank (1), and a liquid outlet valve (15) is provided on the liquid outlet (14); a first motor (16) is provided in the middle of the upper end of the tank (1), and the output end of the first motor (16) is connected to a stirring rod (17) and a plurality of telescopic rods (18), and a pressure plate (19) is fixed at the lower end of each telescopic rod (18); the preparation device also includes a blower (12), the inlet of the blower (12) is connected to the upper part of the end of the puffing conveyor (11), and the outlet is connected to the stirring chamber (5) and the grinding chamber (8) respectively. The side wall of the tank (1) is connected to the second discharge port (10); an electric heater (13) is provided at the output end of the blower (12); a three-way regulating valve (22) is provided at the rear end of the blower (12) to regulate the air flow of the two heating pipes entering the tank (1); a temperature sensor is provided in the stirring chamber (5) to detect the temperature in the stirring chamber (5) and control the blower (12), the electric heater (13) and the three-way regulating valve (22) to provide heat to the stirring chamber (5) to maintain the fermentation temperature.

2. The apparatus for preparing polypeptide bio-expanded compound feed according to claim 1, characterized in that, There are two first feeding ports (2), located at the two ends of the top of the tank body (1); the feeding gate (3) includes a circular sliding ring (301), and the top of the tank body (1) is provided with a groove that cooperates with the sliding ring (301), and the sliding ring (301) slides in contact with the groove; two baffle plates (302) are fixedly connected to the two ends of the sliding ring (301).

3. The apparatus for preparing polypeptide bio-expanded compound feed according to claim 1, characterized in that, The grinding chamber (8) is equipped with a grinding disc (20), and a gravity sensor is installed below the grinding disc (20). When the gravity sensor detects that the weight reaches the set value, the first discharge port (6) is closed.

4. The apparatus for preparing polypeptide bio-expanded compound feed according to claim 1, characterized in that, A second feeding port (21) is provided between the second discharge port (10) and the puffing conveyor (11).

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