In-vitro analytical diagnostic test device and PCR module

Abstract

The present application relates to a kind of in-vitro analysis diagnostic detection device and PCR module, PCR module includes support, temperature rising and falling component and window component.Support is used to install reagent cassette.Temperature rising and falling component includes the substrate on support, the semiconductor refrigerator on substrate, and the heat conduction plate on semiconductor refrigerator.Heat conduction plate is used to be closely contacted with one side of the PCR chamber of reagent cassette.Another side of the PCR chamber is closely contacted with the transparent plate of window component.Optical window is provided with at least one transparent plate.Because the PCR chamber of reagent cassette is slightly inflated under certain pressure, after being compressed by optical window, another side of the PCR chamber is in good contact with heat conduction plate, so that the heat conduction loss between material interface is greatly reduced, the heat conduction efficiency of thermal cycle control of PCR chamber is greatly increased, the temperature of PCR chamber can be accurately controlled, the temperature rising and falling effect is obvious, in addition, the overall structure of PCR module is relatively simple.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of in vitro analysis and diagnosis, in particular to an in vitro analysis and diagnosis detection device and a PCR module. BACKGROUND

[0002] The POCT (point-of-care testing) integrated instrument system based on the rapid detection of the PCR (polymerase chain reaction) technology is suitable for analyzing sample types such as nasopharyngeal swabs, sputum, urine, feces, cervical / vaginal swabs, blood, cerebrospinal fluid, skin, and wound swabs. The instrument system is capable of automatically analyzing the entire process of adding a body fluid sample into a disposable reagent cartridge, placing the cartridge into the instrument system, performing nucleic acid amplification, detection, calculation, and result printing output. Untrained personnel can easily use the instrument system and obtain reliable and accurate detection results. The instrument and reagent are suitable for single or multiple detection of pathogen DNA or RNA that infects the human body.

[0003] In the field of biotechnology, nucleic acid extraction using a centrifugal column method or a magnetic bead method generally requires four steps of lysis, binding, washing, and elution, plus subsequent steps of nucleic acid molecule hybridization, polymerase chain reaction, and molecular detection. Molecular detection technology is developing in the direction of accuracy, convenience, sensitivity, automation, and integration. However, due to the technical complexity of molecular detection, there are few fully automated instrument platforms from sample to result. The structure for raising and lowering the temperature of the sample liquid in the PCR chamber is relatively complex, and the temperature raising and lowering effect is not ideal. SUMMARY

[0004] Therefore, it is necessary to overcome the defects of the prior art and provide an in vitro analysis and diagnosis detection device and a PCR module, which can simplify the structure and improve the temperature raising and lowering effect.

[0005] The technical scheme is as follows: a PCR module, comprising: a support for installing a reagent cartridge; a temperature raising and lowering assembly comprising a substrate provided on the support, a semiconductor refrigerator provided on the substrate, and a heat-conducting plate provided on the semiconductor refrigerator, the heat-conducting plate being used to tightly contact one side of a PCR chamber of the reagent cartridge; and a window assembly comprising an optical window, the optical window being provided with at least one transparent plate, the transparent plate being used to tightly contact the other side of the PCR chamber.

[0006] The PCR module is used, the reagent cartridge is arranged on the support, the heat conduction plate is tightly contacted with one side of the PCR chamber, the transparent plate is tightly contacted with the other side of the PCR chamber, then the semiconductor refrigerator performs the cyclic heating operation and the cooling operation, when heating, the heat is transmitted from the PCR chamber to the base plate through the heat conduction plate, and then transmitted to the heat dissipation fin, the heat is transmitted to the external environment through the heat dissipation fin, so that the PCR chamber is cooled, meanwhile, the excitation light of the optical detection module is transmitted to the sample liquid in the PCR chamber through the transparent plate of the optical window, the sample liquid generates fluorescent reflection to the optical detection module for optical detection, and the sample amplification quantity in each thermal cycle can be quantitatively detected. Since the PCR chamber of the reagent cartridge is slightly inflated under a certain pressure and is compressed by the optical window, the other side of the PCR chamber is well contacted with the heat conduction plate, so that the heat conduction loss between the material interfaces is greatly reduced, the heat conduction efficiency of the thermal cycle control of the PCR chamber is greatly increased, the temperature of the PCR chamber can be accurately controlled, the heating and cooling effect is obvious, and in addition, the overall structure of the PCR module is relatively simple.

[0007] In one of the embodiments, the temperature rising and falling assembly further comprises a heat dissipation member connected with the base plate, and the heat dissipation member comprises a heat dissipation plate stacked on the base plate and a plurality of heat dissipation fins connected with the heat dissipation plate.

[0008] In one of the embodiments, the PCR module further comprises a first pressing assembly, the first pressing assembly comprises a first driving mechanism, a first mounting plate and at least one first pressing member, the first driving mechanism is arranged on the support, the first driving mechanism is connected with the first mounting plate and used to drive the first mounting plate to move towards or away from the reagent cartridge, and the first pressing member is arranged on the first mounting plate and used to press or release the first valve of the reagent cartridge.

[0009] In one of the embodiments, the first pressing assembly further comprises a first air pipe and a second air pipe, the first air pipe and the second air pipe are arranged on the first mounting plate, one end of the first air pipe is provided with a first connector, the other end of the first air pipe is provided with a second connector, one end of the second air pipe is provided with a third connector, and the other end of the second air pipe is provided with a fourth connector, when the first driving mechanism drives the first mounting plate to move to the first position, the first connector and the third connector are respectively used to connect and communicate with the first air hole and the second air hole of the reagent cartridge, and the transparent plate is used to tightly contact with the other side of the PCR chamber.

[0010] The PCR module further comprises a gas path control assembly and a pressure control mechanism, the gas path control assembly is in communication with the second joint, the fourth joint and the pressure control mechanism respectively; the gas path control assembly is provided with a first working state and a second working state; when the gas path control assembly works in the first working state, the second joint is in communication with the pressure control mechanism, and the fourth joint is in communication with the atmosphere; when the gas path control assembly works in the second working state, the second joint is in communication with the atmosphere, and the fourth joint is in communication with the pressure control mechanism.

[0011] In one of the embodiments, the PCR module further comprises a second pressing assembly; the second pressing assembly comprises a second driving mechanism and a second mounting plate; the second driving mechanism is arranged on the support, and the second driving mechanism is connected with the second mounting plate and used to drive the second mounting plate to move towards or away from the reagent cartridge; the window assembly is arranged on the second mounting plate.

[0012] In one of the embodiments, the window assembly further comprises a first elastic member; the optical window is connected with the second mounting plate through the first elastic member.

[0013] In one of the embodiments, the window assembly further comprises a first carrier plate arranged between the first elastic member and the optical window, the optical window is arranged on the first carrier plate, and the first carrier plate is connected with the second mounting plate through the first elastic member.

[0014] In one of the embodiments, the second mounting plate is provided with a first recess, the first elastic member and the first carrier plate are arranged in the first recess; the second mounting plate is provided with a first limiting plate on the plate surface, the first limiting plate is in abutting fit with the first carrier plate, and the first limiting plate is arranged in the circumferential direction around the outer edge of the optical window.

[0015] In one of the embodiments, the second pressing assembly further comprises at least one second pressing member; at least one second pressing member is arranged on the second mounting plate, and the second pressing member is used to press or release the second valve of the reagent cartridge.

[0016] In one of the embodiments, the first pressing assembly further comprises at least one second elastic member arranged on the first mounting plate, at least one first pressing member and at least one second elastic member are arranged one by one, and the first pressing member is connected with the first mounting plate through the second elastic member;

[0017] The second pressing assembly further comprises at least one third elastic member arranged on the second mounting plate, and at least one second pressing member is arranged in one-to-one correspondence with at least one third elastic member, and the second pressing member is connected with the second mounting plate through the third elastic member.

[0018] In one of the embodiments, the first mounting plate is internally provided with a first accommodating chamber and a first guide through hole in communication with the first accommodating chamber, the second elastic member is arranged in the first accommodating chamber, one end of the first pressing member is located in the interior of the first accommodating chamber and connected with the second elastic member, the first pressing member is movably arranged in the first guide through hole, and the other end of the first pressing member extends to the exterior of the first guide through hole and is used for pressing or releasing the first valve of the reagent cartridge.

[0019] The second mounting plate is internally provided with a second accommodating chamber and a second guide through hole in communication with the second accommodating chamber, the third elastic member is arranged in the second accommodating chamber, one end of the second pressing member is located in the interior of the second accommodating chamber and connected with the third elastic member, the second pressing member is movably arranged in the second guide through hole, and the other end of the second pressing member extends to the exterior of the second guide through hole and is used for pressing or releasing the second valve of the reagent cartridge.

[0020] In one of the embodiments, the PCR module further comprises a slide rail assembly, the slide rail assembly comprises a fixing frame arranged on the support, a first slide rail and a second slide rail arranged on the fixing frame, a first sliding block movably arranged on the first slide rail, a second sliding block movably arranged on the second slide rail, a first connecting plate connected with the first sliding block, and a second connecting plate connected with the second sliding block, the first connecting plate is connected with the first mounting plate, and the second connecting plate is connected with the second mounting plate.

[0021] In one of the embodiments, the PCR module further comprises a first sensor and a first trigger member in sensing cooperation with the first sensor, the first sensor is arranged on the support, and the first trigger member is arranged on the first mounting plate or the first connecting plate; the PCR module further comprises a second sensor and a second trigger member in sensing cooperation with the second sensor, the second sensor is arranged on the support, and the second trigger member is arranged on the second mounting plate or the second connecting plate.

[0022] An in-vitro analysis and diagnosis detection device, the in-vitro analysis and diagnosis detection device comprises the PCR module.

[0023] The in-vitro analysis and diagnosis detection device works as follows: the reagent card is installed on the support, so that the heat-conducting plate is in close contact with one side of the PCR chamber, and the transparent plate is in close contact with the other side of the PCR chamber, then the semiconductor refrigerator performs cyclic heating and cooling actions, when heating, heat is transferred to the PCR chamber through the heat-conducting plate, when cooling, heat is transferred from the PCR chamber to the base plate through the heat-conducting plate, and then to the heat sink, so that the heat is transferred to the external environment through the heat sink, thereby cooling the PCR chamber, at the same time, the excitation light of the optical detection module is emitted into the sample liquid in the PCR chamber through the transparent plate of the optical window, the sample liquid generates fluorescence and reflects back to the optical detection module for optical detection, so that the sample amplification quantity in each thermal cycle can be quantitatively detected. Since the PCR chamber of the reagent card is slightly inflated due to a certain pressure, and is compressed by the optical window, the other side of the PCR chamber is in good contact with the heat-conducting plate, thereby greatly reducing the heat conduction loss between the material interfaces, greatly increasing the heat conduction efficiency of the thermal cycle control of the PCR chamber, and accurately controlling the temperature of the PCR chamber, and the heating and cooling effect is obvious, in addition, the overall structure of the PCR module is relatively simple. BRIEF DESCRIPTION OF DRAWINGS

[0024] The accompanying drawings, which form a part of this application, are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification. The drawings illustrate embodiments of the present application and, together with the description, serve to explain the principles of the present application.

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the accompanying drawings needed in the embodiment description will be briefly introduced. Obviously, the accompanying drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without any creative effort.

[0026] Figure 1 It is an embodiment of the PCR module of the present application.

[0027] Figure 2 It is an embodiment of the PCR module of the present application. Figure 1 It is an embodiment of the PCR module of the present application.

[0028] Figure 3 It is an embodiment of the PCR module of the present application. Figure 1 It is an embodiment of the PCR module of the present application.

[0029] Figure 4 It is an embodiment of the reagent card of the present application.

[0030] Figure 5 It is an embodiment of the reagent card of the present application.

[0031] Figure 6 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0032] Figure 7 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application; Figure 6 A cross-sectional view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0033] Figure 8 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0034] Figure 9 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0035] Figure 10 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application; Figure 9 An enlarged view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0036] Figure 11 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application; Figure 10 A cross-sectional view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0037] Figure 12 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0038] Figure 13 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0039] Figure 14 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0040] Figure 15 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application; Figure 14 A cross-sectional view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0041] Figure 16 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0042] Figure 17 Another perspective view of the temperature raising and lowering assembly of the PCR module according to an embodiment of the present application;

[0043] 10, support; 11, support plate; 111, second recess; 112, inlet and outlet; 12, guide plate; 20, temperature rising and falling assembly; 21, base plate; 22, semiconductor refrigerator; 23, heat conduction plate; 24, heat dissipation piece; 25, first positioning plate; 30, window assembly; 31, optical window; 311, transparent plate; 312, PC plate; 32, first elastic piece; 33, first carrier plate; 34, hollowed-out opening; 40, reagent cartridge; 41, first air hole; 42, second air hole; 44, first valve; 45, second valve; 46, sample chamber; 47, pretreatment chamber; 48, mixing chamber; 49, PCR chamber; 50, first pressing assembly; 51, first driving mechanism; 52, first air pipe; 521, first joint; 522, second joint; 523, second step; 53, second air pipe; 531, third joint; 532, fourth joint; 54, first mounting plate; 541, first containing chamber; 542, first guide through hole; 543, third guide through hole; 544, first step; 55, fourth elastic piece; 56, first pressing piece; 57, second elastic piece; 60, second pressing assembly; 61, second driving mechanism; 62, second pressing piece; 63, second mounting plate; 631, first recess; 64, first limiting plate; 70, slide rail assembly; 71, fixing frame; 72, first slide rail; 73, second slide rail; 74, first sliding block; 75, second sliding block; 76, first connecting plate; 77, second connecting plate; 81, first sensor; 82, first trigger piece; 83, second sensor; 84, second trigger piece; 91, thermal cracking assembly; 92, magnetic mixing assembly; 100, optical detection module. DETAILED DESCRIPTION

[0044] In order to make the above objectives, features and advantages of the present application more obvious and easy to understand, the specific embodiments of the present application are described in detail below with reference to the drawings. In the following description, a large number of specific details are set forth in order to fully understand the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the concept of the present application, so the present application is not limited to the specific embodiments disclosed below.

[0045] Referring to Figures 1 to 8 , Figure 1 shows a structure diagram of a PCR module according to an embodiment of the present application, Figure 2 and Figure 3 shows Figure 1 two different structure diagrams of the PCR module after the window assembly 30 and the optical detection module 100 are hidden, Figure 4 shows a structure diagram of a reagent cartridge 40 according to an embodiment of the present application, Figure 5 and Figure 6respectively show two different perspective structural diagrams of the temperature rising and falling assembly 20 of an embodiment of the present application, Figure 7 showed Figure 6 a cross-sectional structural schematic diagram at A-A, Figure 8 A perspective structural schematic diagram of a window assembly 30 of a PCR module of an embodiment is shown installed on one of the second mounting plates 63. An embodiment of the present application provides a PCR module, which includes a support 10, a temperature rising and falling assembly 20, and a window assembly 30. The support 10 is used to install a reagent cartridge 40. The temperature rising and falling assembly 20 includes a substrate 21 arranged on the support 10, a semiconductor refrigerator 22 arranged on the substrate 21, and a heat-conducting plate 23 arranged on the semiconductor refrigerator 22. The heat-conducting plate 23 is used to tightly contact one side of a PCR chamber 49 of the reagent cartridge 40. The window assembly 30 includes an optical window 31. The optical window 31 is provided with at least one transparent plate 311. The transparent plate 311 is used to tightly contact the other side of the PCR chamber 49.

[0046] The PCR module described above is used as follows. The reagent cartridge 40 is installed on the support 10, so that the heat-conducting plate 23 tightly contacts one side of the PCR chamber 49, and the transparent plate 311 tightly contacts the other side of the PCR chamber 49. Then, the semiconductor refrigerator 22 performs cyclic temperature rising and falling actions. When the temperature rises, heat is transferred to the PCR chamber 49 through the heat-conducting plate 23. When the temperature falls, heat is transferred from the PCR chamber 49 to the substrate 21 through the heat-conducting plate 23, and then to the heat dissipation fins, so that the heat is transferred to the external environment by the heat dissipation fins, thereby cooling the PCR chamber 49. At the same time, the excitation light of the optical detection module 100 is emitted into the sample liquid of the PCR chamber 49 through the transparent plate 311 of the optical window 31, the sample liquid generates fluorescent light which is reflected back to the optical detection module 100 for optical detection, so that the sample amplification quantity of each thermal cycle can be quantitatively detected. Since the PCR chamber 49 of the reagent cartridge 40 is slightly inflated due to a certain pressure, and is compressed by the optical window 31, the other side of the PCR chamber 49 has good contact with the heat-conducting plate 23, thereby greatly reducing the heat conduction loss between the material interfaces, greatly increasing the heat conduction efficiency of the thermal cycle control of the PCR chamber 49, accurately controlling the temperature of the PCR chamber 49, and obviously improving the temperature rising and falling effect. In addition, the overall structure of the PCR module is relatively simple.

[0047] It should be noted that the reagent cartridge 40 in the embodiment is provided with at least two chambers, a flow-through passage, a first air hole 41, and a second air hole 42. The at least two chambers are communicated through the flow-through passage. The first air hole 41 and the second air hole 42 are both communicated with the flow-through passage. The specific structural form of the flow-through passage is not limited here, and can be set according to actual needs.

[0048] Please refer to Figure 4In particular, the at least two chambers, for example, include a sample chamber 46, a pretreatment chamber 47, a mixing chamber 48, and a PCR chamber 49, and of course, other functional chambers. The specific number of the sample chamber 46, the pretreatment chamber 47, the mixing chamber 48, and the PCR chamber 49 is not limited and can be set according to actual needs. In the embodiment, the sample chamber 46 is one for accommodating a sample liquid; the pretreatment chamber 47 is one for performing a heating and lysis treatment on the sample liquid; the mixing chamber 48 is, for example, two for mixing the sample liquid with a freeze-dried reagent; and the PCR chamber 49 is, for example, two for performing a polymerase chain reaction and molecular detection treatment on the sample liquid.

[0049] It should be further noted that the number of the transparent plates 311 of the optical window 31 can be one, two, three, or other numbers, which are not limited herein and can be set according to the PCR chamber 49 of the reagent cartridge 40 and set in one-to-one correspondence with the PCR chamber 49.

[0050] Referring to Figures 5 to 7 In addition, the optical window 31 includes a PC plate 312, and the transparent plate 311 is fixed on the PC plate 312 by, for example, screwing, gluing, ultrasonic welding, or laser welding. It should be noted that the transparent plate 311 is specifically selected from an optical material and can be the same as or different from the material of the PC plate 312, which is not limited herein.

[0051] Referring to Figures 8 to 11 , Figure 9 FIG. 6 shows another perspective structural schematic view of the window assembly 30 of the PCR module in an embodiment of the present application installed on the second mounting plate 63, Figure 10 FIG. 7 shows a perspective structural schematic view of the window assembly 30 of the PCR module in an embodiment of the present application installed on the second mounting plate 63, Figure 9 FIG. 8 shows an enlarged structural schematic view of B, Figure 11 FIG. 9 shows an enlarged structural schematic view of C, Figure 10 FIG. 10 shows a sectional structural schematic view of C-C. In an embodiment, the temperature rising and falling assembly 20 further includes a heat dissipation member 24. The heat dissipation member 24 is connected to the base plate 21. The heat dissipation member 24 includes a heat dissipation plate stacked on the base plate 21 and a plurality of heat dissipation fins connected to the heat dissipation plate. In this way, when the semiconductor refrigerator 22 is used to heat and rise the temperature of the PCR chamber 49, the heat dissipation member 24 can better dissipate cold outward through the base plate 21; and when the semiconductor refrigerator 22 is used to cool the PCR chamber 49, the heat dissipation member 24 can better dissipate heat outward through the base plate 21.

[0052] In addition, in order to improve the heat dissipation efficiency of the heat dissipation member 24, the PCR module can also be provided with a fan or a water cooling structure. The fan blows air to the heat dissipation member 24 to quickly take away the heat of the heat dissipation member 24, or the water cooling structure contacts the heat dissipation member 24 to quickly take away the heat of the heat dissipation member 24. In addition, in order to stably arrange the semiconductor refrigerator 22 and the heat conduction plate 23 on the base plate 21, the temperature rising and falling assembly 20 further comprises a first positioning plate 25 fixedly arranged on the base plate 21 by at least one mounting member. The first positioning plate 25 is provided with a first opening, and the semiconductor refrigerator 22 and the heat conduction plate 23 are arranged in the first opening. The edge of the first opening is connected with the outer edge of the heat conduction plate 23 by clamping connection, adhesive connection, or fixed connection by mounting members.

[0053] Please refer to Figures 1 to 4 、 Figures 12 to 15 , Figures 12 to 14 three different perspective structural diagrams of the first mounting plate 54 are shown, Figure 15 Figure 14 a sectional view at D-D. In one embodiment, the PCR module further comprises a first pressing assembly 50. The first pressing assembly 50 comprises a first driving mechanism 51, a first mounting plate 54 and at least one first pressing member 56. The first driving mechanism 51 is arranged on the support 10, and the first driving mechanism 51 is connected with the first mounting plate 54, for driving the first mounting plate 54 to move towards or away from the direction of the reagent cartridge 40. The at least one first pressing member 56 is arranged on the first mounting plate 54, and the first pressing member 56 is used to press or release the first valve 44 of the reagent cartridge 40. Specifically, the first pressing member 56 is, for example, a thimble, a jacking rod, etc., as long as it can push the first valve 44 to make the first valve 44 close, or release the first valve 44 to make the first valve 44 open. The size of the contact end surface of the first pressing member 56 is correspondingly arranged with the size of the first valve 44.

[0054] Please refer to Figure 4 、 Figure 14 and Figure 15 In one embodiment, the first pressing assembly 50 further comprises a first air pipe 52 and a second air pipe 53. The first air pipe 52 and the second air pipe 53 are arranged on the first mounting plate 54. One end of the first air pipe 52 is provided with a first connector 521, the other end of the first air pipe 52 is provided with a second connector 522, one end of the second air pipe 53 is provided with a third connector 531, and the other end of the second air pipe 53 is provided with a fourth connector 532. When the first driving mechanism 51 drives the first mounting plate 54 to move to the first position, the first connector 521 and the third connector 531 are respectively used to connect and communicate with the first air hole 41 and the second air hole 42 of the reagent cartridge 40.

[0055] ​In addition, the PCR module further comprises an air path control assembly (not shown in the figure) and a pressure control mechanism (not shown in the figure). The air path control assembly is in communication with the second joint 522, the fourth joint 532 and the pressure control mechanism respectively. The air path control assembly is provided with a first working state and a second working state. When the air path control assembly works in the first working state, the second joint 522 is controlled to be in communication with the pressure control mechanism, and the fourth joint 532 is controlled to be in communication with the atmosphere. When the air path control assembly works in the second working state, the second joint 522 is controlled to be in communication with the atmosphere, and the fourth joint 532 is controlled to be in communication with the pressure control mechanism.

[0056] Therefore, when the sample liquid in the reagent cartridge 40 needs to be detected, the reagent cartridge 40 is loaded into the support 10, the first driving mechanism 51 moves the first air pipe 52 and the second air pipe 53 to the first position, so that the first joint 521 is in butt joint communication with the first air hole 41, and the third joint 531 is in butt joint communication with the second air hole 42. When the air path control assembly works in the first working state, the first air hole 41 is in butt joint communication with the pressure control mechanism through the first air pipe 52, the second air hole 42 is in communication with the atmosphere through the second air pipe 53, and the suction force provided by the pressure control mechanism acts on the reagent cartridge 40, so that the sample liquid in the reagent cartridge 40 is transferred between the chambers. Similarly, when the air path control assembly works in the second working state, the second air hole 42 is in butt joint communication with the pressure control mechanism through the second air pipe 53, and the first air hole 41 is in communication with the atmosphere through the first air pipe 52. The suction force provided by the pressure control mechanism acts on the reagent cartridge 40, so that the sample liquid in the reagent cartridge 40 is transferred between the chambers. Therefore, by switching the air path control assembly, the first air hole 41 of the reagent cartridge 40 can be in communication with the pressure control mechanism through the first air pipe 52, and the second air hole 42 of the reagent cartridge 40 can be in communication with the pressure control mechanism through the second air pipe 53. The power required by the process of the reagent cartridge 40 can be provided to drive the sample liquid to be transferred between the chambers, and the overall structure of the PCR module is relatively simple.

[0057] Please refer to Figure 4 and Figure 15 In one embodiment, the first joint 521 and the third joint 531 are both vacuum suction cups. Therefore, when the first joint 521 is in butt joint communication with the first air hole 41, the vacuum suction cup completely covers the outside of the hole wall of the first air hole 41, so as to ensure the butt joint sealing between the first joint 521 and the first air hole 41. Similarly, when the third joint 531 is in butt joint communication with the second air hole 42, the vacuum suction cup can completely cover the outside of the hole wall of the second air hole 42, so as to ensure the butt joint sealing between the third joint 531 and the second air hole 42.

[0058] Further, the air path control assembly comprises, for example, electromagnetic control valves and pipelines. The electromagnetic control valves are connected to the second joint 522, the fourth joint 532 and the pressure control mechanism through the pipelines. The pressure control mechanism is specifically a vacuum pump, an air extractor, a plunger pump and the like. The electromagnetic control valves have a first working state and a second working state. When the electromagnetic control valves switch the working state, the first air hole 41 of the reagent card 40 can be communicated with the pressure control mechanism through the first air pipe 52, and the second air hole 42 of the reagent card 40 can be communicated with the pressure control mechanism through the second air pipe 53. It should be noted that the number of electromagnetic control valves, the specific structure of the pipeline and the setting position of the electromagnetic control valve on the pipeline are not limited here, and can be set according to actual needs as long as at least the first working state and the second working state can be realized. For example, there are two electromagnetic control valves. When one of the electromagnetic control valves acts, the second joint 522 can be communicated with the air pump through the pipeline or with the atmosphere through the pipeline, and the other electromagnetic control valve acts synchronously, so that the fourth joint 532 can be communicated with the atmosphere through the pipeline or with the air pump through the pipeline.

[0059] Please refer to Figures 1 to 2 、 Figures 8 to 10 In one embodiment, the PCR module further comprises a second pressing assembly 60. The second pressing assembly 60 comprises a second driving mechanism 61 and a second mounting plate 63. The second driving mechanism 61 is arranged on the support 10, and the second driving mechanism 61 is connected to the second mounting plate 63 and used to drive the second mounting plate 63 to move towards or away from the reagent card 40. The window assembly 30 is arranged on the second mounting plate 63. In this way, the second driving mechanism 61 drives the second mounting plate 63 to move towards or away from the reagent card 40, and the second mounting plate 63 drives the window assembly 30 to move when the second mounting plate 63 moves, so that the transparent plate 311 of the window assembly 30 can tightly abut against the other side of the PCR chamber 49 or release the other side of the PCR chamber 49. That is, when the reagent card 40 is disassembled on the support 10, the transparent plate 311 is driven by the second driving mechanism 61 to move away from the other side of the PCR chamber 49, so as to avoid interference with the reagent card 40; when the reagent card 40 is assembled in place on the support 10, the transparent plate 311 is driven by the second driving mechanism 61 to tightly abut against the other side of the PCR chamber 49.

[0060] It should be noted that the first driving mechanism 51 is, for example, a motor-screw driving structure, a cylinder driving structure, a hydraulic cylinder driving structure, an electric cylinder driving structure, a cam driving structure and the like, which is not limited here and can be set according to actual needs. The second driving mechanism 61 is similar to the first driving mechanism 51, which will not be described here.

[0061] Please refer toFigure 4 、 Figure 8 With Figure 11 In one embodiment, the window assembly 30 further comprises a first elastic member 32. The optical window 31 is connected to the second mounting plate 63 through the first elastic member 32. In this way, the first elastic member 32 plays a buffering role in the process that the transparent plate 311 of the optical window 31 contacts the side of the PCR chamber 49, ensuring that the transparent plate 311 tightly contacts the side of the PCR chamber 49 while avoiding damage to the PCR chamber 49.

[0062] Referring to Figure 4 、 Figure 8 With Figure 11 In one embodiment, the window assembly 30 further comprises a first carrier plate 33 arranged between the first elastic member 32 and the optical window 31. The optical window 31 is arranged on the first carrier plate 33, and the first carrier plate 33 is connected to the second mounting plate 63 through the first elastic member 32. Specifically, the first carrier plate 33 is made of a hard material such as aluminum alloy, which can strengthen the structural strength of the optical window 31 and is not prone to damage. In addition, the number of the first elastic member 32 is not limited here, for example, it can be one, two, three, four or other numbers, which can be set according to actual needs. In addition, it should be noted that in order to let the fluorescence of the optical detection module 100 pass through the transparent plate 311 of the optical window 31, the PC board 312 of the second mounting plate 63, the first carrier plate 33 and the optical window 31 are provided with a hollow opening 34 corresponding to the position of the transparent plate 311, which can let the fluorescence of the optical detection module 100 pass through.

[0063] Referring to Figure 4 、 Figure 8 With Figure 11 In one embodiment, the second mounting plate 63 is provided with a first recess 631, and the first elastic member 32 and the first carrier plate 33 are arranged in the first recess 631. The surface of the second mounting plate 63 is provided with a first limiting plate 64, the first limiting plate 64 is in abutting fit with the first carrier plate 33, and the first limiting plate 64 is arranged circumferentially around the outer edge of the optical window 31. In this way, on the one hand, in the process that the transparent plate 311 abuts against the side of the PCR chamber 49, the first carrier plate 33 will move in the first recess 631 under the thrust, and the running stability is good; on the other hand, the first limiting plate 64 plays a limiting role on the first carrier plate 33, which can avoid the first carrier plate 33 and the first elastic member 32 from being separated from the first recess 631.

[0064] In one embodiment, the second pressing assembly 60 further comprises at least one second pressing member 62. The at least one second pressing member 62 is arranged on the second mounting plate 63, and the second pressing member 62 is used to press or loosen the second valve 45 of the reagent cartridge.

[0065] Referring to Figure 4 ,Figure 8 With Figure 12 In one embodiment, the number of the first pressing members 56 mounted on the first mounting plate 54 is not less than the number of the first valves 44 of the reagent cartridge 40, and the number of the second pressing members 62 mounted on the second mounting plate 63 is not less than the number of the second valves 45 of the reagent cartridge 40. In this way, when the first driving mechanism 51 drives the first mounting plate 54 to move, all the first valves 44 of the reagent cartridge 40 can be opened or closed. Similarly, when the second driving mechanism 61 drives the second mounting plate 63 to move, all the second valves 45 of the reagent cartridge 40 can be opened or closed. For example, the number of the first pressing members 56 in the embodiment is four, three of which are arranged one-to-one with the three first valves 44 of the reagent cartridge 40, and the other one is also used to press or release the reagent cartridge 40, but not at the position where the first valve 44 is arranged, so as to ensure better pressing effect on the side surface of the reagent cartridge 40. In addition, the number of the second pressing members 62 in the embodiment is four, for example, which are arranged one-to-one with the four second valves 45 of the reagent cartridge 40.

[0066] As an optional solution, the first pressing members 56 of the first pressing assembly 50 and the second pressing members 62 of the second pressing assembly 60 can be arranged on the same mounting plate, i.e. without arranging two mounting plates, and the mounting plate on which the first pressing members 56 and the second pressing members 62 are arranged can be driven to move by one driving mechanism. In addition, a third pressing assembly or a fourth pressing assembly, etc. can also be arranged, which is not limited herein.

[0067] In one embodiment, the first pressing assembly 50 further comprises at least one second elastic member 57 mounted on the first mounting plate 54. The at least one first pressing member 56 and the at least one second elastic member 57 are arranged one-to-one, and the first pressing member 56 is connected to the first mounting plate 54 through the second elastic member 57.

[0068] In addition, the second pressing assembly 60 further comprises at least one third elastic member (not shown in the figure) mounted on the second mounting plate 63, and the at least one second pressing member 62 and the at least one third elastic member are arranged one-to-one, and the second pressing member 62 is connected to the second mounting plate 63 through the third elastic member.

[0069] Referring to Figure 15In one embodiment, the first mounting plate 54 is internally provided with a first accommodating chamber 541 and a first guide through hole 542 in communication with the first accommodating chamber 541, the second elastic member 57 is arranged in the first accommodating chamber 541, one end of the first pressing member 56 is located in the interior of the first accommodating chamber 541 and connected with the second elastic member 57, the first pressing member 56 is movably arranged in the first guide through hole 542, and the other end of the first pressing member 56 extends to the outside of the first guide through hole 542 and is used for pressing or releasing the first valve 44 of the reagent card box 40.

[0070] In addition, the second mounting plate 63 is internally provided with a second accommodating chamber and a second guide through hole (not shown in the figure) in communication with the second accommodating chamber, a third elastic member is arranged in the second accommodating chamber, one end of the second pressing member 62 is located in the interior of the second accommodating chamber and connected with the third elastic member, the second pressing member 62 is movably arranged in the second guide through hole, and the other end of the second pressing member 62 extends to the outside of the second guide through hole and is used for pressing or releasing the second valve 45 of the reagent card box 40.

[0071] In one embodiment, please refer to Figures 1 to 3 , Figure 16 and Figure 17 , Figure 16 and Figure 17 respectively show the structural schematic diagrams of two different views of the slide rail assembly 70. In one embodiment, the PCR module further comprises a slide rail assembly 70. The slide rail assembly 70 comprises a fixed frame 71 arranged on the support 10, a first slide rail 72 and a second slide rail 73 arranged on the fixed frame 71, a first sliding block 74 movably arranged on the first slide rail 72, a second sliding block 75 movably arranged on the second slide rail 73, a first connecting plate 76 connected with the first sliding block 74, and a second connecting plate 77 connected with the second sliding block 75. The first connecting plate 76 is connected with the first mounting plate 54, and the second connecting plate 77 is connected with the second mounting plate 63. In this way, when the first driving mechanism 51 drives the first mounting plate 54 to move, the first connecting plate 76 drives the first sliding block 74 to move along the first slide rail 72, so that the operation stability of the first mounting plate 54 is better, and the movement stroke of the first mounting plate 54 can be more accurately controlled, that is, the movement stroke accuracy of the first pressing member 56 reaches 0.05mm. In addition, since the movement stroke accuracy of the first pressing member 56 is higher, when the first valve 44 is opened, the first pressing member 56 moves away from the first valve 44 by a distance of, for example, 0.1mm or 0.2mm under the power of the first driving mechanism 51 to release the first valve 44 of the reagent card box 40, at this time, the first pressing member 56 still abuts against the side surface of the reagent card box 40 to play a positioning role, so that the reagent card box 40 is stably arranged on the support 10.

[0072] When the second driving mechanism 61 drives the second mounting plate 63 to move, the second connecting plate 77 drives the second sliding block 75 to move along the second sliding rail 73, so that the operation stability of the second mounting plate 63 is better, and the movement stroke of the second mounting plate 63, i.e. the movement stroke of the second pressing piece 62, is more accurately controlled, and the movement stroke accuracy of the second pressing piece 62 reaches 0.05 mm. Because the movement stroke accuracy of the second pressing piece 62 is higher, when the second valve 45 is opened, the second pressing piece 62 moves away from the second valve 45 by a distance of, for example, 0.1 mm or 0.2 mm under the power of the second driving mechanism 61 to loosen the second valve 45 of the reagent card box 40, at this time, the second pressing piece 62 still abuts against the side of the reagent card box 40 to play a positioning role, so that the reagent card box 40 is stably arranged on the support 10, and in addition, the first connector 521 and the third connector 531 still respectively maintain the butt joint communication with the first gas hole 41 and the second gas hole 42 of the reagent card box 40, and the transparent plate 311 still abuts against the other side of the PCR chamber 49.

[0073] Specifically, the first mounting plate 54, the second mounting plate 63, the first sliding block 74, the second sliding block 75, the first connecting plate 76 and the second connecting plate 77 are specifically, for example, aluminum, copper, iron, stainless steel, wood, plastic and the like, which are not limited here and can be set according to actual needs.

[0074] It should be noted that the “first connecting plate 76” can be “a part of the first sliding block 74”, that is, the “first connecting plate 76” is integrally formed with “other parts of the first sliding block 74”; or it can be a separate component that can be separated from “other parts of the first sliding block 74”, that is, the “first connecting plate 76” can be independently manufactured and then combined with “other parts of the first sliding block 74” to form a whole.

[0075] Referring to Figure 2 And Figure 3 In an embodiment, the PCR module further comprises a first sensor 81 and a first trigger 82 that cooperates with the first sensor 81 for sensing. The first sensor 81 is arranged on the support 10, and the first trigger 82 is arranged on the first mounting plate 54 or the first connecting plate 76. In this way, the first sensor 81 can sense the moving position of the first trigger 82, and according to the sensing signal of the first trigger 82, the moving position information of the first mounting plate 54 can be obtained, and then the movement stroke of the first pressing piece 56 can be correspondingly controlled within a preset range.

[0076] In addition, the PCR module further comprises a second sensor 83 and a second trigger 84 that cooperates with the second sensor 83 for sensing. The second sensor 83 is arranged on the support plate 11, and the second trigger 84 is arranged on the second mounting plate 63 or the second connecting plate 77.

[0077] Specifically, the first sensor 81 is a photoelectric switch, a proximity switch, a reflection sensor or other types of sensors, as long as it can sense the moving position of the first trigger 82 and timely feedback the moving position signal of the first trigger 82. The second sensor 83 is similar to the first sensor 81, and will not be described here.

[0078] In one embodiment, the first pressing assembly 50 further comprises at least one second elastic member 57 arranged on the first mounting plate 54. The at least one first pressing member 56 is arranged in one-to-one correspondence with the at least one second elastic member 57, and the first pressing member 56 is connected to the first mounting plate 54 through the second elastic member 57. In this way, under the buffering action of the second elastic member 57, the first pressing member 56 can avoid causing damage to the reagent cartridge 40 during contact with the first valve 44.

[0079] Similarly, the second pressing assembly 60 further comprises at least one third elastic member arranged on the second mounting plate 63, and the at least one second pressing member 62 is arranged in one-to-one correspondence with the at least one third elastic member. The second pressing member 62 is connected to the second mounting plate 63 through the third elastic member. Similarly, under the buffering action of the third elastic member, the second pressing member 62 can avoid causing damage to the reagent cartridge 40 during contact with the second valve 45.

[0080] Please refer to Figure 14 With Figure 15 In one embodiment, the first mounting plate 54 is internally provided with a first accommodating chamber 541 and a first guide through hole 542 in communication with the first accommodating chamber 541. The second elastic member 57 is arranged in the first accommodating chamber 541, one end of the first pressing member 56 is located in the interior of the first accommodating chamber 541 and connected to the second elastic member 57, the first pressing member 56 is movably arranged in the first guide through hole 542, and the other end of the first pressing member 56 extends to the outside of the first guide through hole 542 and is used for pressing or loosening the first valve 44 of the reagent cartridge 40.

[0081] The second mounting plate 63 is internally provided with a second accommodating chamber (not shown in the figure) and a second guide through hole (not shown in the figure) in communication with the second accommodating chamber. The third elastic member is arranged in the second accommodating chamber. One end of the second pressing member 62 is located in the interior of the second accommodating chamber and connected to the third elastic member, the second pressing member 62 is movably arranged in the second guide through hole, and the other end of the second pressing member 62 extends to the outside of the second guide through hole and is used for pressing or loosening the second valve 45 of the reagent cartridge 40.

[0082] In one embodiment, the first pressing assembly 50 further comprises a fourth elastic member 55. The first air pipe 52 is connected with the first mounting plate 54 through the fourth elastic member 55. In this way, during the abutting connection of the first joint 521 of the first air pipe 52 with the first air hole 41 of the reagent card 40 under the pushing of the first driving mechanism 51, the fourth elastic member 55 plays a buffering role to avoid damage to the reagent card 40 by the first joint 521.

[0083] In one embodiment, the first mounting plate 54 is provided with a third guide through hole 543, the first air pipe 52 is movably arranged in the third guide through hole 543, the hole wall of the third guide through hole 543 is provided with a first step 544, and the outer wall of the first air pipe 52 is provided with a second step 523. The fourth elastic member 55 has two ends respectively corresponding to the first step 544 and the second step 523.

[0084] Specifically, the fourth elastic member 55 is a spring sleeved on the outer wall of the air pipe, and the two ends of the spring correspond to the first step 544 and the second step 523 respectively.

[0085] Specifically, the first elastic member 32, the second elastic member 57, the third elastic member and the fourth elastic member 55 are, for example, springs, elastic blocks and the like, which are not limited here and can be set according to actual needs.

[0086] It should be noted that the installation mode of the second air pipe 53 on the first mounting plate 54 is similar to that of the first air pipe 52, which will not be described here.

[0087] Referring to Figures 1 to 3 Further, the support 10 comprises a support plate 11 and a guide plate 12. One side surface of the support plate 11 is provided with a second recess 111 for adapting to the reagent card 40. The guide plate 12 is arranged on one side surface of the support plate 11 and is used to abut against the side surface of the reagent card 40 loaded into the second recess 111. One side wall of the second recess 111 is provided with an entrance and exit 112. In this way, when the reagent card 40 needs to be tested, the reagent card 40 enters the second recess 111 along the guide plate 12 through the entrance and exit 112, that is, the guide plate 12 plays a guiding role for the reagent card 40, and the position of the reagent card 40 is relatively stable under the action of the guide plate 12 and the support plate 11 after entering the second recess 111.

[0088] Please refer to Figure 2In one embodiment, the PCR module further comprises a thermal lysis assembly 91. The thermal lysis assembly 91 is arranged on the support plate 11 and is used to perform thermal lysis treatment on the liquid in the pre-treatment chamber 47 of the reagent cartridge 40. In addition, the PCR module further comprises a magnetic mixing assembly 92. The magnetic mixing assembly 92 is arranged on the support plate 11 and is used to make the magnetic beads in the mixing chamber 48 of the reagent cartridge 40 move up and down, so that the liquid in the mixing chamber 48 is mixed sufficiently to obtain the required mixed liquid.

[0089] Referring to Figure 3 , Figure 8 and Figure 12 In one embodiment, in order to avoid the first pressing member 56 or the air tube from interfering with the guide plate 12 during the process of pressing against the side surface of the reagent cartridge 40, at least one first through hole (not shown in the figure) and at least one second through hole (not shown in the figure) are arranged on the guide plate 12. The at least one first through hole is arranged in one-to-one correspondence with the at least one first pressing member 56 and can allow the first pressing member 56 to pass through. The at least one second through hole is arranged in correspondence with the first joint 521 of the first air tube 52 and also in correspondence with the third joint 531 of the second air tube 53, and can allow the first joint 521 to pass through and the third joint 531 to pass through.

[0090] In one embodiment, the PCR module further comprises a first sensor 81 and a first trigger member 82 that is in sensing cooperation with the first sensor 81. The first sensor 81 is arranged on the bracket 10, and the first trigger member 82 is arranged on the first mounting plate 54 or the first connecting plate 76. In this way, the first sensor 81 can sense the moving position of the first trigger member 82, and according to the sensing signal of the first trigger member 82, the moving position information of the first mounting plate 54 can be obtained, and then the movement stroke of the first pressing member 56 can be controlled within a preset range.

[0091] In addition, the PCR module further comprises a second sensor 83 and a second trigger member 84 that is in sensing cooperation with the second sensor 83. The second sensor 83 is arranged on the support plate 11, and the second trigger member 84 is arranged on the second mounting plate 63 or the second connecting plate 77.

[0092] Specifically, the first sensor 81 is an optical switch, a proximity switch, a reflection sensor or other types of sensors, as long as it can sense the moving position of the first trigger member 82 and timely feedback the moving position signal of the first trigger member 82. The second sensor 83 is similar to the first sensor 81, and will not be described here.

[0093] Referring to Figures 1 to 8 In one embodiment, an in-vitro analysis and diagnosis detection device comprises the PCR module of any of the above embodiments.

[0094] In the working process of the in-vitro analysis and diagnosis detection device, the reagent cartridge 40 is installed on the support 10, so that the heat-conducting plate 23 tightly contacts one side of the PCR chamber 49, and the transparent plate 311 tightly contacts the other side of the PCR chamber 49. Then, the semiconductor refrigerator 22 performs cyclic temperature rising and falling actions. In the temperature rising process, heat is transferred to the PCR chamber 49 through the heat-conducting plate 23. In the temperature falling process, heat is transferred from the PCR chamber 49 to the base plate 21 through the heat-conducting plate 23, and then to the heat dissipation fins, so that the heat is transferred to the external environment through the heat dissipation fins, thereby cooling the PCR chamber 49. At the same time, the excitation light of the optical detection module 100 is emitted into the sample liquid in the PCR chamber 49 through the transparent plate 311 of the optical window 31, the sample liquid generates fluorescence and reflects back to the optical detection module 100 for optical detection, so that the sample amplification quantity in each thermal cycle can be quantitatively detected. Since the PCR chamber 49 of the reagent cartridge 40 is slightly inflated due to a certain pressure, and the other side of the PCR chamber 49 is compressed by the optical window 31, the other side of the PCR chamber 49 has good contact with the heat-conducting plate 23, thereby greatly reducing the heat conduction loss between the material interfaces, greatly increasing the heat conduction efficiency of the thermal cycle control of the PCR chamber 49, accurately controlling the temperature of the PCR chamber 49, and obviously improving the temperature rising and falling effect. In addition, the overall structure of the PCR module is relatively simple.

[0095] In one embodiment, the PCR module further comprises a temperature sensor (not shown in the figure). The temperature sensor is used to obtain the temperature information of the heat-conducting plate 23. In this way, the temperature information of the heat-conducting plate 23 is sensed in real time through the temperature sensor, so that the temperature of the heat-conducting plate 23 can be accurately grasped, and the semiconductor refrigerator 22 can be controlled to work according to the detected temperature information.

[0096] Generally, a temperature sensor is installed on a heating body to monitor the temperature of the heating body. Due to the reliability of the performance of the temperature sensor and the reliability of the production and installation process, there is a certain temperature difference between the actual temperature and the set temperature, which affects the reliability of the test results of the sample liquid.

[0097] Further, the temperature sensor is two, and the two temperature sensors are connected with the heat-conducting plate 23. The wires of the two temperature sensors are also used to be electrically connected with a controller (not shown in the figure). In this way, the semiconductor refrigerator 22 synchronously compares the temperature values returned by the two temperature sensors during the temperature control and adjustment of the heat-conducting plate 23. If the two detected temperature values are out of tolerance, an error is reported, so that it can be ensured that the temperature sensor is not failed. In addition, for example, the temperature of the heat-conducting plate 23 is controlled through a PID algorithm, so as to ensure the accuracy and reliability of the temperature of the heat-conducting plate 23.

[0098] Any technical features in the above-described embodiments can be combined in any manner, and for the sake of brevity, not all possible combinations are described, but it is understood that the scope of the present disclosure encompasses all possible combinations.

[0099] The above-described embodiments are merely exemplary and do not limit the scope of the present disclosure. It should be understood that various modifications and alterations can be made by those skilled in the art without departing from the spirit of the present disclosure. Accordingly, the scope of the present disclosure should be determined by reasonable interpretation of the appended claims and the technical scope of the present disclosure.

[0100] In the description of the present disclosure, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like are used to indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are merely used for the convenience of describing the present disclosure and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present disclosure.

[0101] In addition, the terms "first", "second", "third", etc. are used only for descriptive purposes and should not be construed as indicating or implying relative importance or a specific number of the technical features indicated. Therefore, the features defined with "first", "second", etc. can explicitly or implicitly include at least one of the features. In the description of the present disclosure, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise explicitly specified.

[0102] In the present disclosure, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting", "fixing" and the like should be understood in a broad sense, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present disclosure can be understood according to the specific circumstances.

[0103] In the present application, unless explicitly stated and limited otherwise, a first feature "on", "above", or "under" a second feature can be directly in contact with the second feature, or indirectly in contact with the second feature through an intermediate medium. Also, a first feature "over", "above", and "on top of" a second feature can be directly above or diagonally above the second feature, or simply mean that the first feature is horizontally higher than the second feature. A first feature "under", "below", and "underneath" a second feature can be directly below or diagonally below the second feature, or simply mean that the first feature is horizontally lower than the second feature.

[0104] It is to be understood that when an element is referred to as being "on" or "connected to" another element, it can be directly on or connected to the other element or intervening elements can be present. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items. Other than in the operating examples or where otherwise indicated, as used herein, the terms "comprising", "comprises" and "comprised of" are to be construed as meaning "including, but not limited to".

Claims

1. A PCR module, characterized in that, The PCR module comprises: a support for mounting a reagent cartridge; a temperature raising and lowering assembly comprising a base plate arranged on the support, a semiconductor refrigerator arranged on the base plate, and a heat conduction plate arranged on the semiconductor refrigerator, the heat conduction plate being arranged to tightly contact one side of a PCR chamber of the reagent cartridge; a window assembly comprising an optical window, the optical window being provided with at least one transparent plate arranged to tightly contact the other side of the PCR chamber; a first pressing assembly comprising a first driving mechanism arranged on the support, a first mounting plate connected to the first driving mechanism and arranged to move towards or away from the reagent cartridge, and at least one first pressing member arranged on the first mounting plate and arranged to press or release a first valve of the reagent cartridge; and a second pressing assembly comprising a second driving mechanism arranged on the support, a second mounting plate connected to the second driving mechanism and arranged to move towards or away from the reagent cartridge, and the window assembly arranged on the second mounting plate.

2. The PCR module of claim 1, wherein, The temperature raising and lowering assembly further comprises a heat dissipation member connected to the base plate, the heat dissipation member comprising a heat dissipation plate arranged on the base plate, and a plurality of heat dissipation fins connected to the heat dissipation plate.

3. The PCR module of claim 1, wherein, The first pressing assembly further comprises a first air pipe and a second air pipe, both of which are arranged on the first mounting plate, one end of the first air pipe being provided with a first connector, the other end of the first air pipe being provided with a second connector, one end of the second air pipe being provided with a third connector, and the other end of the second air pipe being provided with a fourth connector, the first connector and the third connector being arranged to be connected to a first air hole and a second air hole of the reagent cartridge, respectively, when the first driving mechanism drives the first mounting plate to move to a first position. The PCR module further comprises an air path control assembly and a pressure control mechanism, the air path control assembly being connected to the second connector, the fourth connector and the pressure control mechanism, respectively, the air path control assembly being provided with a first working state and a second working state, the second connector being connected to the atmosphere and the fourth connector being connected to the pressure control mechanism when the air path control assembly works in the first working state, and the second connector being connected to the atmosphere and the fourth connector being connected to the pressure control mechanism when the air path control assembly works in the second working state.

4. The PCR module of claim 1, wherein, The window assembly further comprises a first elastic member, the optical window being connected to the second mounting plate through the first elastic member.

5. The PCR module of claim 4, wherein, The window assembly further comprises a first carrier plate arranged between the first elastic member and the optical window, the optical window being arranged on the first carrier plate, and the first carrier plate being connected to the second mounting plate through the first elastic member.

6. The PCR module of claim 5, wherein, The second mounting plate is provided with a first recess, and the first elastic member and the first carrier plate are arranged in the first recess; a first limiting plate is arranged on the surface of the second mounting plate, the first limiting plate is in abutting fit with the first carrier plate, and the first limiting plate is arranged circumferentially around the outer edge of the optical window.

7. The PCR module of claim 1, wherein, The second pressing assembly further comprises at least one second pressing member, and the at least one second pressing member is arranged on the second mounting plate, and the second pressing member is used for pressing or releasing the second valve of the reagent cartridge.

8. The PCR module of claim 7, wherein, The first pressing assembly further comprises at least one second elastic member arranged on the first mounting plate, and the first pressing member and the second elastic member are arranged one by one, and the first pressing member is connected to the first mounting plate through the second elastic member. The second pressing assembly further comprises at least one third elastic member arranged on the second mounting plate, and the second pressing member and the third elastic member are arranged one by one, and the second pressing member is connected to the second mounting plate through the third elastic member.

9. The PCR module of claim 8, wherein, The first mounting plate is internally provided with a first accommodating chamber and a first guide through hole in communication with the first accommodating chamber, the second elastic member is arranged in the first accommodating chamber, one end of the first pressing member is located in the interior of the first accommodating chamber and connected to the second elastic member, the first pressing member is movably arranged in the first guide through hole, and the other end of the first pressing member extends to the exterior of the first guide through hole and is used for pressing or releasing the first valve of the reagent cartridge. The second mounting plate is internally provided with a second accommodating chamber and a second guide through hole in communication with the second accommodating chamber, the third elastic member is arranged in the second accommodating chamber, one end of the second pressing member is located in the interior of the second accommodating chamber and connected to the third elastic member, the second pressing member is movably arranged in the second guide through hole, and the other end of the second pressing member extends to the exterior of the second guide through hole and is used for pressing or releasing the second valve of the reagent cartridge.

10. The PCR module of claim 1, wherein, The PCR module further comprises a slide rail assembly, the slide rail assembly comprises a fixing frame arranged on the bracket, a first slide rail and a second slide rail arranged on the fixing frame, a first sliding block movably arranged on the first slide rail, a second sliding block movably arranged on the second slide rail, a first connecting plate connected to the first sliding block, and a second connecting plate connected to the second sliding block; the first connecting plate is connected to the first mounting plate, and the second connecting plate is connected to the second mounting plate.

11. The PCR module of claim 10, wherein, The PCR module further comprises a first sensor and a first trigger matched with the first sensor; the first sensor is arranged on the support, and the first trigger is arranged on the first mounting plate or the first connecting plate; the PCR module further comprises a second sensor and a second trigger matched with the second sensor; the second sensor is arranged on the support, and the second trigger is arranged on the second mounting plate or the second connecting plate.

12. An in vitro analytical diagnostic test device, characterized in that The in-vitro analysis and diagnosis detection device comprises the PCR module according to any one of claims 1 to 11.

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