A nematocide composition and its application

Abstract

The present invention discloses a nematocide composition and its application. The composition contains active ingredient A and active ingredient B, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 20:1; the active ingredient A is Cyclobutrifluram, and the active ingredient B is flupyradifurone. The nematocide composition of the present invention can be used to control plant parasitic nematodes, has low toxicity, is environmentally friendly, and can effectively reduce production costs and usage costs.

Description

[0001] This divisional application of the present invention has an application number of CN202210034388.1, a filing date of January 13, 2022, and an invention title of "A Nematicidal Composition and Its Application". Technical Field

[0002] The present invention belongs to the technical field of pesticide compounding, and particularly relates to a nematicidal composition and its application. Background Art

[0003] Plant parasitic nematodes are a type of worm that can parasitize various plant tissues such as plant roots, stems, and leaves. Currently, they have become the second largest category of pathogens harming agricultural production. Plant parasitic nematodes can not only infect hosts through secretions but also spread other plant pathogens, causing secondary plant diseases. There are more than 200 genera of plant parasitic nematodes, involving more than 5,000 species. The main plant parasitic nematodes that harm agricultural and forestry production include root-knot nematodes (Meloidogyne), cyst nematodes (Heterodera), aphelenchid nematodes (Aphelenchina), stem nematodes (Ditylenchus), root nodule nematodes (Nacobbus), root rot nematodes (Pratylenchus), pine wood nematodes (Bursa phelenchus xylophilus), etc., with cyst nematodes (Heterodera) and root-knot nematodes (Meloidogyne) being the main ones.

[0004] Cyclobutrifluram is a novel nicotinamide bactericidal and nematicidal agent developed by Syngenta. IUPAC name: N-[2-(2,4-dichlorophenyl)cyclobutyl]-2-(trifluoromethyl)nicotinamide, which contains 80%-100% of the (1S,2S)-enantiomer and 0-20% of the (1R,2R)-enantiomer; CAS registration number: 1460292-16-3; molecular formula: C 17 H 13 CI 2 F 3 N 2 O. Cyclobutrifluram has a broad control spectrum, high efficiency, low dosage, is easy to use, can effectively control various nematodes and major fungal diseases, is highly effective against root-knot nematodes, beet cyst nematodes, and corn lesion nematodes on crops such as cucumbers, tomatoes, corn, and beets, can be used for soil treatment or seed treatment, and can provide long-term control of nematodes and diseases in major crops and various environments, especially having excellent control effects against Fusarium.

[0005] By measuring the bioactivity of compounds against target organisms and designing different pesticide combinations, it is one of the effective methods to improve the control effect and delay drug resistance. The inventors of the present invention have conducted in-depth research on Cyclobutrifluram and flupyradifurone or acetoprole and their combinations, and found that when Cyclobutrifluram is compounded with any one of flupyradifurone or acetoprole, it has an obvious synergistic effect within a certain proportion range, and there are differences in the action mechanisms of Cyclobutrifluram and flupyradifurone or acetoprole. Compounding Cyclobutrifluram with any one of flupyradifurone or acetoprole is beneficial to overcome and delay the drug resistance of nematodes, reduce the dosage of pesticides, lower the cost, and improve the control effect. Summary of the Invention

[0006] The object of the present invention is to provide a nematicidal composition with high activity against nematodes, which can improve the actual control effect, effectively slow down the generation of drug resistance of target pests, and reduce the occurrence of soil-borne diseases.

[0007] To achieve the above object, the present invention is realized through the following technical solutions: a nematicidal composition and its application, the composition contains active ingredient A and active ingredient B, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 20:1; the active ingredient A is Cyclobutrifluram, and the active ingredient B is any one of flupyradifurone and acetoprole;

[0008] Further, the active ingredient B is flupyradifurone, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 5:1;

[0009] Furthermore, the mass ratio of Cyclobutrifluram to flupyradifurone is 1:20, 1:10, 1:5, 1:3, 1:2, 1:1, 2:1, 3:1, 5:1;

[0010] Further, the mass ratio of Cyclobutrifluram to flupyradifurone is 1:10 to 3:1;

[0011] Furthermore, the mass ratio of Cyclobutrifluram to flupyradifurone is 1:10, 1:5, 1:3, 1:2, 1:1, 2:1, 3:1;

[0012] Further, the mass ratio of Cyclobutrifluram to flupyradifurone is 1:3 to 2:1;

[0013] Furthermore, the mass ratio of Cyclobutrifluram to flupyradifurone is 1:3, 1:2, 1:1, 2:1;

[0014] Furthermore, the mass ratio of Cyclobutrifluram to flupyradifurone is 1:3 to 1:1;

[0015] More specifically, the mass ratio of Cyclobutrifluram to flupyradifurone is 1:3, 1:2, 1:1;

[0016] Furthermore, the active ingredient B is acetoprole, and the mass ratio of active ingredient A to active ingredient B is 1:10 to 10:1;

[0017] More specifically, the mass ratio of Cyclobutrifluram to acetoprole is 1:10, 1:5, 1:3, 1:2, 1:1, 2:1, 3:1, 5:1, 10:1;

[0018] Furthermore, the mass ratio of Cyclobutrifluram to acetoprole is 1:5 to 5:1;

[0019] More specifically, the mass ratio of Cyclobutrifluram to acetoprole is 1:5, 1:3, 1:2, 1:1, 2:1, 3:1, 5:1;

[0020] Furthermore, the mass ratio of Cyclobutrifluram to acetoprole is 1:3 to 3:1;

[0021] More specifically, the mass ratio of Cyclobutrifluram to acetoprole is 1:3, 1:2, 1:1, 2:1, 3:1;

[0022] The composition contains active ingredients and also includes agriculturally acceptable auxiliary ingredients; the total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the composition;

[0023] Furthermore, the total weight of active ingredient A and active ingredient B accounts for 1% to 50% of the composition;

[0024] Furthermore, the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, preservatives, stabilizers, synergists, carriers;

[0025] Furthermore, the wetting agent is selected from one or more of sodium dodecylbenzene sulfonate, sodium dodecylbenzenesulfonate, saponin powder, alkyl sulfates, Nekal BX, silkworm excrement, alkylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkanolamide polyoxyethylene ether and its phosphate or sulfate salt, alkyl polyoxyethylene ether sulfosuccinate;

[0026] The dispersant is selected from one or more of polycarboxylates, lignosulfonates, naphthalene or alkylnaphthalene formaldehyde condensate sulfonates, calcium alkylbenzenesulfonate, alkylphenol polyoxyethylene phosphate, fatty alcohol polyoxyethylene polyoxypropylene ether, fatty alcohol polyoxypropylene polyoxypropylene ether, and polymeric alkylaryl sulfonates;

[0027] The emulsifier is selected from one or more of fatty alcohol polyoxyethylene ethers, fatty alcohol ethylene oxide - propylene oxide copolymers, phenethylphenol polyoxyethylene polyoxypropylene ethers, alkylphenol polyoxyethylene ethers, fatty amine polyoxyethylene ethers, alkylbenzenesulfonates, styrylphenol polyoxyethylene ethers, and fatty acid polyoxyethylene esters;

[0028] The thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, and carboxymethyl cellulose;

[0029] The disintegrant is selected from one or more of aluminum chloride, bentonite, sucrose, modified starch, cellulose, urea, sodium carbonate, sodium bicarbonate, sodium chloride, sodium sulfate, citric acid, and tartaric acid;

[0030] The antifreeze is selected from one or more of alcohols, alcohol ethers, and inorganic salts;

[0031] The defoamer is selected from one or more of silicone oil, C 10 ~C 20 saturated fatty acid compounds, C 8 ~C 10 fatty alcohol compounds, or silicone compounds;

[0032] The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n - butanol, diesel, N,N - dimethylformamide, cyclohexanone, ethyl acetate, N - methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives, and deionized water;

[0033] The preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p - hydroxybenzoate, and methyl p - hydroxybenzoate;

[0034] The stabilizer is selected from one or more of oxalic acid, succinic acid, adipic acid, borax, and epoxidized vegetable oil;

[0035] The carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomite, and silica white;

[0036] Furthermore, the composition can be prepared into any preparation dosage form permitted in agriculture;

[0037] Furthermore, the preparation dosage form is a granule, a suspending agent, or an emulsifiable concentrate;

[0038] Application of the nematocide composition and / or its preparation as described above in controlling plant pests.

[0039] Furthermore, the plant pests are nematodes, and the nematodes are any one or more of root-knot nematodes (Meloidogyne), cyst nematodes (Heterodera), aphelenchid nematodes (Aphelenchina), stem nematodes (Ditylenchus), root nodule nematodes (Nacobbus), root rot nematodes (Pratylenchus), and pine wood nematodes (Bursaphelenchus xylophilus);

[0040] Even further, the nematodes are root-knot nematodes (Meloidogyne);

[0041] Furthermore, the plants include but are not limited to crops cultivated in protected fields and field crops, such as cucumber, towel gourd, balsam pear, watermelon, strawberry, tomato, celery, peanut, tobacco, sweet potato, ginger, rice, soybean, banana, sugarcane, potato, citrus, kiwifruit, and wheat.

[0042] The present invention has the following advantages compared with the prior art:

[0043] 1) The composition of the present invention is a compound mixture with different action mechanisms, which can delay the generation of nematode drug resistance, reduce environmental pressure, and extend the service life of the compound;

[0044] 2) The composition of the present invention has good nematocidal activity;

[0045] 3) The composition of the present invention has low toxicity, little residue in crops, can greatly reduce the dosage of pesticides in the field, reduce production and use costs, and effectively reduce environmental pollution and pesticide residues. Detailed implementation manners

[0046] In order to better illustrate the effective control effect of the present invention, the above-mentioned example agents are used to make the technical solutions, purposes, and advantages of the present invention clearer. The present invention is illustrated by the following specific examples, but the present invention is not limited to these examples. The effect experiments of the present invention adopt a combination of indoor bioassays and field trials.

[0047] Formulation preparation examples:

[0048] Preparation process of the preparation examples:

[0049] 1. Suspending agent formulation processing technology: According to the formula ratio, the technical material, surfactant and other functional additives are successively placed in a reaction kettle, mixed evenly with water, and then subjected to high-speed shearing and wet grinding, and finally homogenized and filtered to obtain the required suspending agent.

[0050] 2. Emulsifiable concentrate formulation processing technology: According to the formula ratio, the metered technical material, solvent and cosolvent are added to a blending kettle and stirred to dissolve, then the emulsifier is added, and the remaining solvent is used to make up the balance. After stirring evenly in the stirring kettle and filtering, the required emulsifiable concentrate is obtained.

[0051] 3. Granule formulation processing technology: Using the coating granulation method, the technical material, dispersant and additive are mixed evenly to make mother powder, and the mother powder and carrier are added to a coating granulator and stirred. While stirring, the prepared binder aqueous solution is added. After fully mixing evenly, the material is discharged, and after drying and sieving, the granule described in the present invention can be obtained.

[0052] Preparation Example 1: 15% Cyclobutrifluram·flufenoxystrobin suspending agent (1:2)

[0053] Component Content Cyclobutrifluram 5％ Fluxapyroxad 10％ Fatty alcohol polyoxyethylene ether 1％ Styrylphenol polyoxyethylene ether phosphate 3％ Sodium polycarboxylate 1％ Xanthan gum 1％ Alkylaryl polyoxyethylene polyoxypropylene ether 2％ Ethylene glycol 5％ Magnesium aluminum silicate 1％ Xanthan gum 0.3％ Sodium benzoate 0.5％ Organic silicon defoamer 0.5％ Deionized water Make up the balance

[0054] Preparation Example 2: 30% Cyclobutrifluram·flufenoxystrobin emulsifiable concentrate (1:1)

[0055]

[0056]

[0057] Preparation Example 3: 16% Cyclobutrifluram·flufenoxystrobin granule (1:3)

[0058] Component Content Cyclobutrifluram 4％ Fluxapyroxad 12％ Sodium polycarboxylate 1％ Polyvinyl alcohol 2％ Sodium lignosulfonate 3％ Silica white 8％ Ceramsite particles Make up the balance

[0059] Preparation Example 4: 18% Cyclobutrifluram·acetoprole suspending agent (1:2)

[0060] Component Content Cyclobutrifluram 6％ Acetoprole 12％ Fatty alcohol polyoxyethylene ether 1％ Alkylphenol polyoxyethylene ether phosphate 3％ Sodium lignosulfonate 1％ Xanthan gum 0.25％ Magnesium aluminum silicate 1％ Glycerol 5％ Sodium sorbate 1％ Silicone oil 0.5％ Deionized water Make up the balance

[0061] Preparation Example 5: 20% Cyclobutrifluram·acetoprole emulsifiable concentrate (1:1)

[0062] Component Content Cyclobutrifluram 10％ Acetoprole 10％ Propylene glycol methyl ether 20％ Alkylphenol polyoxyethylene ether 10％ Calcium dodecylbenzenesulfonate 3％ Cyclohexanone 20％ Trimethylbenzene Make up the balance

[0063] Preparation Example 6: 30% Cyclobutrifluram·flufenoxystrobin granule (2:1)

[0064] Component Content Cyclobutrifluram 20％ Fluxapyroxad 10％ Sodium lignosulfonate 3％ Polyvinyl acetate 3％ Silica white 8％ Diatomite particles Make up the balance

[0065] Indoor Bioassay Test

[0066] Example 1: Joint Toxicity Determination Test of Cyclobutrifluram and Flupyradifurone or Acephate on Meloidogyne incognita

[0067] Test Basis: The test refers to NY / T 1833.1-2009 "Pesticide Indoor Bioassay Test Standard Nematicide Part 1: Test for Inhibiting Phytopathogenic Nematodes - Insect Immersion Method".

[0068] Test Object: Meloidogyne incognita (Kofold & White) Chitwood, isolated from the root knot samples of greenhouse cucumbers in Yifengdian, Jimo, Qingdao, and cultured and propagated on living cucumber plants in the laboratory.

[0069] Test Agents: 90% cyclobutrifluram technical, 95% flupyradifurone technical, 96% acephate technical, all provided by the Group R & D Center.

[0070] Agent Preparation: First dissolve each of the above technical agents in acetone, and then dilute with 0.1% Tween - 80 aqueous solution. Set 5 series of concentration gradients for each agent, and use an aqueous solution without the agent and with the same solvent content as the control.

[0071] Preparation of Nematode Suspension: Pick root knot nematode egg masses from severely diseased cucumber root knots, wash them with clean water, place them on filter paper in a petri dish, add an appropriate amount of distilled water, and incubate in an incubator at 25 ± 1°C. After 24h, collect second - instar larvae with consistent instars to prepare a root knot nematode suspension, with no less than 100 nematodes per 1 mL of suspension for standby.

[0072] Test Method: Use a pipette to successively suck 3 mL of the liquid medicine from low concentration to high concentration and add them into test tubes, then suck 3 mL of the prepared equal - volume nematode suspension and add it into the test tubes to mix the liquid medicine and the nematode suspension evenly. Use a pipette gun to transfer a certain volume of the mixed liquid into the wells of a multi - well biochemical test plate, cover it, and repeat each treatment 4 times. Use an aqueous solution without the agent and with the same solvent content as the control. Incubate at 25°C for 24h, check the activity of the nematodes, and calculate the relative mortality rate of each agent treatment on the nematodes.

[0073] Test Investigation: Take 1 mL of the mixed liquid from each treatment and observe the death of nematodes under a dissecting microscope. The number of nematodes observed for each repeat is not less than 100. Record the total number of nematodes investigated and the number of dead nematodes. The dead nematodes are often stiff and cannot bend or move when touched with a hairpin or bamboo needle.

[0074] Calculation Method:

[0075] According to the survey data, calculate the mortality rate of each treatment, and calculate according to the following formula. The calculation results are all rounded to two decimal places:

[0076]

[0077]

[0078] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 10%, correction should be made according to the corrected mortality rate formula; if the control mortality rate is greater than 15%, the test needs to be redone.

[0079] Statistical analysis: According to the logarithmic values of each pesticide concentration and the corresponding mortality rates, analyze with the IBM SPSS Statistics 20 statistical analysis system to obtain the toxicity regression line, LC 50 value and the correlation coefficient R 2 , and evaluate the activity of the tested pesticides on the biological test materials.

[0080] Calculate the co-toxicity coefficient (CTC) of the mixture according to the Sun Yunpei method to evaluate the type of joint action.

[0081] CTC≥120 indicates a synergistic effect, CTC≤80 indicates an antagonistic effect, and CTC between 80 and 120 indicates an additive effect.

[0082] The co-toxicity coefficient (CTC value) of the mixture is calculated by the following formula:

[0083]

[0084] In the formula:

[0085] ATI——Actual toxicity index of the mixture;

[0086] S——LC of the standard insecticide 50 , in milligrams per liter (mg / L);

[0087] M——LC of the mixture 50 , in milligrams per liter (mg / L).

[0088] TTI = TI A *P A +TI B *P B

[0089] In the formula:

[0090] TTI——Theoretical toxicity index of the mixture;

[0091] TI A ——Toxicity index of pesticide A;

[0092] PA —— Percentage content of Agent A in the mixture, in percentage (%)

[0093] TI B —— Toxicity index of Agent B

[0094] P B —— Percentage content of Agent B in the mixture, in percentage (%)

[0095]

[0096] Wherein:

[0097] CTC - Co - toxicity coefficient

[0098] ATI - Measured toxicity index of the mixture

[0099] TTI - Theoretical toxicity index of the mixture

[0100] The test results are shown in the following table.

[0101] Table 1 Test results of joint toxicity determination of the mixture of Cyclobutrifluram and flufenoxystrobin against Meloidogyne incognita

[0102] Test agents and ratios Regression equation <![CDATA[Coefficient of correlation R 2 > <![CDATA[LC 50 (mg / L)]]> Coefficient of co-toxicity Clclobutrifluram(A) y = 1.380x - 0.531 0.986 2.426 - Fluxapyroxad(B) y = 1.693x - 1.482 0.982 7.505 - A:B = 1:20 y = 1.673x - 1.201 0.993 5.223 130.665 A:B = 1:10 y = 1.620x - 1.035 0.993 4.355 144.776 A:B = 1:5 y = 1.796x - 0.984 0.998 3.532 157.522 A:B = 1:3 y = 1.785x - 0.749 0.994 2.628 187.462 A:B = 1:2 y = 1.467x - 0.446 0.989 2.014 219.478 A:B = 1:1 y = 1.516x - 0.412 0.999 1.870 196.082 A:B = 2:1 y = 1.565x - 0.367 0.989 1.716 182.557 A:B = 3:1 y = 1.569x - 0.386 0.992 1.762 165.723 A:B = 5:1 y = 1.267x - 0.374 0.982 1.973 138.592 A:B = 10:1 y = 1.228x - 0.393 0.992 2.089 123.745 A:B = 20:1 y = 1.899x - 0.604 0.992 2.080 120.518

[0103] It can be seen from Table 1 that when the mass ratio of Cyclobutrifluram to flufenoxystrobin is 1:20 - 20:1, it has a good control effect on Meloidogyne incognita, and the combined effect shows synergism; when the mass ratio of Cyclobutrifluram to flufenoxystrobin is 1:20 - 5:1, its co - toxicity coefficient is greater than 130, and the synergistic effect is significant; when the mass ratio of Cyclobutrifluram to flufenoxystrobin is 1:5 - 3:1, its co - toxicity coefficient is greater than 150, and the synergistic effect is prominent; when the mass ratio of Cyclobutrifluram to flufenoxystrobin is 1:2, the co - toxicity coefficient is the largest at 219.478, indicating that the mixture of Cyclobutrifluram and flufenoxystrobin has the best activity against Meloidogyne incognita at this mass ratio.

[0104] Table 2 Test results of joint toxicity determination of the mixture of Cyclobutrifluram and acetoprole against Meloidogyne incognita

[0105] Test agents and ratios Regression equation <![CDATA[Coefficient of correlation R 2 > <![CDATA[LC 50 (mg / L)]]> Coefficient of co-toxicity Clclobutrifluram(A) y = 2.014x - 0.801 0.997 2.499 - Acetoprole(B) y = 1.477x - 1.473 0.989 9.940 - A:B = 1:20 y = 1.915x - 1.614 0.996 6.965 124.991 A:B = 1:10 y = 1.609x - 1.235 0.999 5.858 133.536 A:B = 1:5 y = 1.487x - 0.927 0.995 4.203 158.059 A:B = 1:3 y = 1.478x - 0.809 0.986 3.528 161.515 A:B = 1:2 y = 1.395x - 0.596 0.992 2.675 186.491 A:B = 1:1 y = 1.438x - 0.438 0.995 2.016 198.110 A:B = 2:1 y = 1.505x - 0.336 0.985 1.672 199.158 A:B = 3:1 y = 1.469x - 0.374 0.988 1.797 171.083 A:B = 5:1 y = 1.443x - 0.412 0.996 1.930 147.940 A:B = 10:1 y = 1.316x - 0.404 0.995 2.028 132.223 A:B = 20:1 y = 1.393x - 0.455 0.998 2.122 122.119

[0106] As can be seen from Table 2, when the mass ratio of Cyclobutrifluram to clothianidin is from 1:20 to 20:1, it has a good control effect on Meloidogyne incognita, and the combined effect shows synergism; when the mass ratio of Cyclobutrifluram to clothianidin is from 1:10 to 10:1, its co-toxicity coefficient is greater than 130, and the synergistic effect is significant; when the mass ratio of Cyclobutrifluram to clothianidin is from 1:5 to 5:1, its co-toxicity coefficient is greater than 140, and the synergistic effect is prominent; when the mass ratio of Cyclobutrifluram to clothianidin is 2:1, the co-toxicity coefficient is the largest at 199.158, indicating that the combination of Cyclobutrifluram and clothianidin has the best activity against Meloidogyne incognita at this mass ratio.

[0107] Field efficacy test

[0108] Example 2: Field efficacy test of the combination of Cyclobutrifluram and flufenoxuron or clothianidin against Meloidogyne incognita on cucumber roots

[0109] Test object: Meloidogyne incognita Chitwood on cucumber roots; after isolation and identification, it is mainly Meloidogyne incongnita (Kofold & White) Chitwood.

[0110] Test site: Cucumber greenhouse in Liujia Village, Ershilidian Town, Haiyang City, Yantai City, Shandong Province. The test field is for protected cultivation. Meloidogyne incognita has occurred severely and evenly over the years. The cultivation conditions in each plot are uniform and consistent, meeting the local conventional cultivation practices.

[0111] Test crop and variety: Cucumber (Improved Cuiyu).

[0112] Test agents: The test agents and the application rates of their preparations are shown in the following table.

[0113] Test design: The test plots are arranged in a randomized block design, with each plot having an area of 20 m 2 , and each treatment is replicated 4 times. After the cucumber seedlings were transplanted and established (on July 6, 2020), the medicine was applied once by the method of irrigation / topdressing.

[0114] Investigation method: The efficacy was investigated once before pulling out the cucumber plants (on November 13, 2020); suspicious diseased plants in the plot were found, and by the diagonal five-point sampling method, two plants were investigated at each point to calculate the disease index and control effect.

[0115] Diseased plant grading method:

[0116] Grade 0: No galls on the roots;

[0117] Level 1: A small number of galls on the root system;

[0118] Level 3: Two-thirds of the root system is covered with small galls;

[0119] Level 5: The root system is covered with small galls and has secondary galls;

[0120] Level 7: The root system forms a fibrous root mass.

[0121] Method for calculating drug efficacy:

[0122]

[0123]

[0124] The test results are shown in the following table:

[0125] Table 3

[0126]

[0127] It can be seen from the above table that the nematicidal compositions with different ratios have good control effects on Meloidogyne incognita of cucumber. The control effects of each preparation example on Meloidogyne incognita of cucumber are all higher than 81%. Among them, 20% Cyclobutrifluram·acetoprole emulsifiable concentrate (1:1) and 15%

[0128] Cyclobutrifluram·flufenoxuron suspension concentrate (1:2) have the most significant control effects on Meloidogyne incognita of cucumber, and their control effects are 87.02% and 85.58% respectively.

[0129] To sum up, through indoor toxicity determination and field efficacy tests, the nematicidal composition of the present invention has good control effects on nematodes, is safe for target crops, has significant control effects, is superior to single agents in delaying the generation of drug resistance and prolonging the persistence, and can effectively reduce the production cost and use cost.

Claims

1. A nematocide composition, characterized in that the composition contains active ingredient A and active ingredient B, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 20:1; the active ingredient A is Cyclobutrifluram, and the active ingredient B is flupyradifurone.

2. The nematocide composition according to claim 1, characterized in that the mass ratio of active ingredient A to active ingredient B is 1:10 to 10:

1.

3. The nematocide composition according to claim 1, characterized in that the mass ratio of active ingredient A to active ingredient B is 1:5 to 5:

1.

4. The nematocide composition according to claim 1, characterized in that the total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the composition.

5. The nematocide composition according to claim 4, characterized in that the total weight of active ingredient A and active ingredient B accounts for 1% to 50% of the composition.

6. The nematocide composition according to claim 1, characterized in that in addition to the active ingredient, the composition further includes auxiliary ingredients permitted in agriculture.

7. The nematocide composition according to claim 6, characterized in that the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, preservatives, stabilizers, synergists, carriers.

8. The nematocide composition according to claim 1, characterized in that the composition can be prepared into any preparation dosage form permitted in agriculture.

9. The nematocide composition according to claim 8, characterized in that the preparation dosage form is a granule, a suspension or an emulsifiable concentrate.

10. The application of the nematocide composition and / or its preparation according to any one of claims 1-9 in controlling plant pests, characterized in that the plant pests are Meloidogyne incognita.

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