Gordonia fibrolytica and its applications

Abstract

The present invention discloses Gordonia cellulolyticum and its applications. The Gordonia cellulolyticum G22706 provided by the present invention has the registration number of CGMCC No. 25246 in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms. The Gordonia cellulolyticum G22706 provided by the present invention is a new species of the genus Gordonia, and at the same time, through the detection of cellulose degradation ability, it is also proved that this strain has cellulose degradation activity. This bacterium has broad application prospects in food processing, garbage treatment, soil remediation, soil improvement, etc.

Description

Technical Field

[0001] The present invention relates to the field of microorganisms, and particularly to Gordonia fibrolytica and its applications. Background Art

[0002] The genus Gordonia belongs to the domain Bacteria - phylum Actinobacteria - class Actinomycetia - order Corynebacteriales - family Gordoniaceae. The genus Gordonia was initially proposed by Tsukamura in 1971 and named Gordon (Tsukamura M. Proposal of a new genus, Gordona, for slightly acid-fast organisms occurring in sputa of patients with pulmonary disease and in soil. J Gen Microbiol. 1971 Sep;68(1):15 - 26. doi:10.1099 / 00221287 - 68 - 1 - 15. PMID:4109926.), and was renamed Gordonia in 1997 (Stackebrandt E, Rainey F A, Ward - Rainey N L. Proposal for a New Hierarchic Classification System, Actinobacteria classis nov[J]. International Journal of Systematic Bacteriology, 1997, 47(2):479 - 491.). Strains of the genus Gordonia have a wide range of sources. The initial reports were isolates from clinical samples. Subsequently, there have been studies on isolates from environments such as soil, ocean, biofiltration devices, industrial wastewater, sewage treatment reactors, rocks, mangrove ecosystems, soil, deserts, rhizosphere of plants, fresh water, and human body fluids. Currently, there are 47 validly described species in this genus, and the type strain is Gordonia amicalis HS - 11 T .

[0003] Previous studies have shown that multiple members of the genus Gordonia have the ability to produce secondary metabolites, enzymes, surfactants, etc., and play important roles in the discovery of bioactive secondary metabolites, plant growth promotion, and ecological environment protection. The strain Gordonia sp. 647W.R.1a.05 isolated from trypanosomes can secrete various substances with neuroactive or antibacterial activities, such as cyclomycin A, dimycin, 1,4-diphenyl-2,3-butanediol, etc., which have similar effects to the trypanotoxin secreted by its host (Lin Z, et al. Neuroactive diol and acyloin metabolites from cone snail-associated bacteria. Bioorg Med Chem Lett. 2013 Sep 1; 23(17): 4867-9. doi: 10.1016 / j.bmcl.2013.06.088. Epub 2013 Jul 8. PMID: 23880542.). The strain Gordonia sp. S2RP-17 isolated from the rhizosphere of the plant Equisetum arvense planted in petroleum-contaminated soil can produce siderophores and deaminated-1-aminocyclopropane-1-carboxylate. Siderophores can convert the extremely insoluble iron element in the soil into a soluble state, promote the absorption of iron by plants, and play a role in promoting plant growth (Hong SH, et al. Rhizoremediation of diesel-contaminated soil using the plant growth-promoting rhizobacterium Gordonia sp. S2RP-17. Biodegradation. 2011 Jun; 22(3): 593-601. doi: 10.1007 / s10532-010-9432-2. Epub 2010 Nov 17. PMID: 21082332.). Moreover, some Gordonia can directly synthesize auxin to promote plant growth.Gordonia sp. JPA2, isolated from the rhizosphere of Chenopodium murale growing in salt pans in Haryana, India, can not only tolerate up to 6% NaCl but also produce PGP (plant growth-promoting) substances such as indole-3-acetic acid, ammonia, and siderophores. Therefore, this strain is also considered a potential candidate for developing bioinoculants suitable for saline-alkali soils (Hong SH, et al. Rhizoremediation of diesel-contaminated soil using the plant growth-promoting rhizobacterium Gordonia sp. S2RP-17. Biodegradation. 2011 Jun;22(3):593-601. doi:10.1007 / s10532-010-9432-2. Epub 2010 Nov 17. PMID:21082332.). A strain of Gordonia isolated from a forest has the potential to degrade lignin and alkanes and exhibits high phenol oxidase and peroxidase activities (Woo HL, et al. Enzyme activities of aerobic lignocellulolytic bacteria isolated from wet tropical forest soils. Syst Appl Microbiol. 2014 Feb;37(1):60-7. doi:10.1016 / j.syapm.2013.10.001. Epub 2013 Nov 14. PMID:24238986.). A new strain of Gordonia, G. namibiensis NAM-BN063A, was isolated from a sandy soil sample in the Waterberg Kalahari Desert in central Namibia and can degrade a variety of nitrile compounds. In recent years, microbial oil recovery and microbial remediation technologies for pollutants, which have the significant advantages of high efficiency, environmental protection, and economy with microbial degradation as the core, have received extensive attention from scholars. Therefore, Gordonia strains have potential application value in the field of microbial remediation. Summary of the Invention

[0004] The object of the present invention is to provide a new species of Gordonia and its applications.

[0005] In the first aspect, the present invention claims protection for a new species of Gordonia.

[0006] The novel species of the genus Gordonia protected by the present invention is specifically Gordonia cellulolyticum G22706, and its registration number in the China General Microbiological Culture Collection Center is CGMCC No. 25246.

[0007] The Gordonia cellulolyticum G22706 is a Gram-positive aerobic heterotrophic bacterium. The cells are short rods with a size of (0.2 - 0.3) × (0.5 - 1) μm, without spore formation and non-motile. After culturing on PYG medium at 28 °C for 5 days, circular, raised, and dry-surfaced light orange colonies with a diameter of approximately (1.5 - 3) mm are formed, and no soluble pigment is produced. The growth tolerance range of the strain is 15 - 37 °C, 0 - 10% NaCl, and pH 4.0 - 10.0, and the optimal growth conditions are 28 - 37 °C, 0% NaCl, and pH 7.0 - 8.0. It is catalase-positive, gelatin liquefaction-negative, and does not produce indole and H2S. The production of alkaline phosphatase, esterase C4, lipase C14, leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, chymotrypsin, α-galactosidase, β-galactosidase, β-glucuronidase, α-glucosidase, and β-glucosidase is positive. The 16S rRNA sequence of this strain is as shown in SEQ ID No. 1.

[0008] In the second aspect, the present invention claims to protect a culture.

[0009] The culture protected by the present invention is the culture of Gordonia cellulolyticum G22706 described in the first aspect above, specifically the substance obtained by culturing the Gordonia cellulolyticum G22706 in a bacterial medium.

[0010] In the above culture, the substance includes the Gordonia cellulolyticum G22706 (the cells themselves) and the metabolites of the Gordonia cellulolyticum G22706.

[0011] In the above culture, the bacterial medium can be a solid medium or a liquid medium.

[0012] The term "culture" generally refers to a liquid or solid medium with a microbial population after artificial inoculation and cultivation. That is, a product obtained by growing and / or amplifying microorganisms, which can be a pure biological culture of microorganisms or can contain a certain amount of medium, metabolites, or other components produced during the cultivation process. The term "culture" also includes subcultures obtained by subculturing microorganisms, which can be a culture of a certain generation or a mixture of several generations.

[0013] In a specific embodiment of the present invention, the bacterial medium is specifically a PYG solid medium.

[0014] In a third aspect, the present invention claims to protect a metabolite.

[0015] The metabolite claimed to be protected by the present invention is the metabolite of Gordonia cellulolyticum G22706 described in the first aspect above.

[0016] The term "metabolite" refers to primary metabolites and / or secondary metabolites produced during the metabolism of microorganisms. Primary metabolism refers to the process by which microorganisms absorb various nutrients from the outside world and generate substances and energy for maintaining life activities through catabolism and anabolism. The products of primary metabolism are primary metabolites, such as monomers of monosaccharides or monosaccharide derivatives, nucleotides, vitamins, amino acids, fatty acids, etc., and various macromolecular polymers composed of them, such as proteins, nucleic acids, polysaccharides, lipids, etc. Secondary metabolism refers to the process by which microorganisms synthesize some substances with no clear function for the life activities of microorganisms using primary metabolites as precursors during a certain growth period. The products of secondary metabolism are secondary metabolites, most of which are compounds with relatively complex molecular structures. According to their functions, they can be classified into types such as antibiotics, hormones, alkaloids, toxins, etc.

[0017] In a fourth aspect, the present invention claims to protect a bacterial agent.

[0018] The bacterial agent claimed to be protected by the present invention contains Gordonia cellulolyticum G22706 described in the first aspect above, the culture described in the second aspect above, and / or the metabolite described in the third aspect above.

[0019] The bacterial agent is a bacterial agent for hydrolyzing cellulose.

[0020] In the above-mentioned bacterial agent, in addition to the active ingredient, the bacterial agent also contains a carrier. The carrier can be a carrier commonly used in the pesticide field and biologically inert. The carrier can be a solid carrier or a liquid carrier; the solid carrier can be a mineral material, a plant material or a polymer compound; the mineral material can be at least one of clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica and diatomaceous earth; the plant material can be at least one of corn flour, bean flour and starch; the polymer compound can be polyvinyl alcohol and / or polyglycol; the liquid carrier can be an organic solvent, vegetable oil, mineral oil or water; the organic solvent can be decane and / or dodecane.

[0021] In the above-mentioned bacterial agent, the dosage form of the bacterial agent can be various dosage forms, such as liquid agent, emulsion, suspension, powder, granule, wettable powder or water dispersible granule.

[0022] According to needs, surfactants (such as Tween 20, Tween 80, etc.), binders, stabilizers (such as antioxidants), pH regulators, etc. can also be added to the bacterial agent.

[0023] In the fifth aspect, the present invention claims the application of the Gordonia cellulolyticum G22706 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above in any of the following:

[0024] (A1) Hydrolyzing cellulose;

[0025] (A2) Preparing a product for hydrolyzing cellulose;

[0026] (A3) Preparing cellulase;

[0027] (A4) Preparing a product with cellulase activity.

[0028] In the sixth aspect, the present invention claims the application of the Gordonia cellulolyticum G22706 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above in any of the following:

[0029] (B1) Food processing;

[0030] (B2) Garbage treatment;

[0031] (B3) Soil remediation;

[0032] (B4) Soil quality improvement.

[0033] In the seventh aspect, the present invention claims a product for hydrolyzing cellulose.

[0034] The product for hydrolyzing cellulose claimed by the present invention has an active ingredient which is Gordonia cellulolyticum G22706 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above.

[0035] In the eighth aspect, the present invention claims a product having cellulase activity.

[0036] The product having cellulase activity claimed by the present invention has an active ingredient which is Gordonia cellulolyticum G22706 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above.

[0037] In the ninth aspect, the present invention claims a method for hydrolyzing cellulose.

[0038] The method for hydrolyzing cellulose claimed by the present invention may include the following steps: treating a sample to be processed with Gordonia cellulolyticum G22706 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above.

[0039] In the tenth aspect, the present invention claims the use of Gordonia cellulolyticum G22706 described in the first aspect above in the preparation of the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above.

[0040] Experimental results have shown that there are many significant differences between Gordonia cellulolyticum G22706 provided by the present invention and existing Gordonia strains in terms of phenotypic, physiological and biochemical, and cytochemical characteristics. Meanwhile, phylogenetic analysis at the gene level further illustrates the differences between this strain and the existing published Gordonia species, fully demonstrating that Gordonia cellulolyticum G22706 provided by the present invention represents a new species of the genus Gordonia. At the same time, through the detection of cellulose degradation ability, it is also proved that the strain of the present invention, Gordonia cellulolyticum G22706, has cellulose degradation activity. This bacterium has broad application prospects in food processing, garbage treatment, soil remediation, soil improvement and other aspects.

[0041] Depository Instructions

[0042] Classification name: Gordonia cellulolyticum;

[0043] Biological material referred to: G22706;

[0044] Depositary institution: China General Microbiological Culture Collection Center;

[0045] Abbreviation of the depositary institution: CGMCC;

[0046] Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing;

[0047] Date of deposit: July 7, 2022;

[0048] Registration number in the deposit center: CGMCC No. 25246. Description of the drawings

[0049] Figure 1 It is a colony photograph of strain G22706 cultured on PYG medium at 28°C for 5 days.

[0050] Figure 2 It is a screening diagram of the cellulose degradation ability of strain G22706.

[0051] Figure 3 It is an N-J phylogenetic tree constructed based on the 16S rRNA gene sequences of strain G22706 and related strains of the genus Gordonia, showing the phylogenetic status of strain G22706. Detailed implementation manners

[0052] The present invention will be further described in detail below in combination with specific implementation manners. The examples given are only for clarifying the present invention, rather than limiting the scope of the present invention. The following examples can be used as a guide for those of ordinary skill in the art to make further improvements, and do not limit the present invention in any way.

[0053] The experimental methods in the following examples are all conventional methods unless otherwise specified, and are carried out according to the techniques or conditions described in the literature in this field or according to the product instructions. The materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

[0054] Example 1 Isolation and identification of Gordonia cellulolyticum G22706 I. Isolation of strain G22706

[0055] The strain G22706 of the present invention was isolated from the soil of Kubuqi Desert in Inner Mongolia Autonomous Region. The specific isolation operation is as follows:

[0056] 1. Preparation of soil suspension

[0057] Take a small amount of soil sample and air-dry it for 1 week. Then, heat-treat it in an oven at 80 °C for 15 min. After that, add 2 g of the soil sample to 18 mL of a sterile mixed solution containing 0.1% sodium pyrophosphate and 0.85% sodium chloride. Place it in a shaker at 28 °C and shake at 180 rpm for 40 min to fully suspend the soil sample. Gradient-dilute the formed soil suspension with a mixed solution containing 0.1% sodium pyrophosphate and 0.85% sodium chloride to 10 -4 for subsequent sub-culturing of bacteria.

[0058] 2. Strain isolation medium

[0059] Use M2 isolation medium, and the nutrient components are as follows: sodium propionate 2 g / L, NH4NO3 0.1 g / L, KCl 0.1 g / L, MgSO4·7H2O 0.05 g / L, FeSO4·7H2O 0.05 g / L, marine trace salts 0.38 g / L, agar 15 g / L, and make up to 1 L with deionized water; pH 7 - 8.

[0060] 3. Isolation method

[0061] Spread the above-diluted soil suspension on the M2 isolation medium plate and culture it at 28 °C for 3 weeks. Pick out single colonies with good growth, complete bacterial lawns, and different morphological characteristics, and culture them on the PYG slant medium (formula: peptone 3 g·L -1 , yeast extract 5 g·L -1 , glycerol 10 g·L -1 , betaine 1.25 g·L -1 , sodium pyruvate 1.25 g·L -1 , agar 15 g·L -1 ; pH 7) to obtain pure cultures of different strains for subsequent research. At the same time, the obtained pure strains are preserved in liquid nitrogen at ultra-low temperature and frozen at -80 °C with 20% (v / v) glycerol as a cryoprotectant. After amplifying and sequencing the 16S rRNA gene of the pure cultures after morphological duplicate elimination, use EZBioCloud (https: / / www.ezbiocloud.net / ) for comparison to obtain a strain with a similarity of 98.4% to the closest reference strain Gordonia bronchialis DSM 43247 T . The strain number is G22706, and it is preliminarily determined to be a potential new species.

[0062] II. Identification of strain G22706

[0063] The strain G22706 was grown on PYG medium at 28 °C, and morphological, physiological and biochemical, cytochemical, and genetic level studies were carried out. Other special circumstances will be described.

[0064] 1. Detection of morphological, physiological and biochemical characteristics of strain G22706

[0065] After the strain G22706 was cultured on PYG solid medium at 28 °C for 5 days, the colony morphology was observed using an automatic colony counter (Xunshu) and a stereomicroscope (OLYMPUS SZX7). The growth temperature detection range was 4, 10, 15, 20, 25, 28, 30, 37, 42, and 45 °C; the growth salt concentration (NaCl) detection range was 12 concentration gradients of 0-10% and 15% (0-10 g / 100 mL and 15 g / 100 mL) (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 15); the growth pH detection range was 7 gradients (4, 5, 6, 7, 8, 9, 10) between 4 and 10 (Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol 2005; 55: 1149-1153). The physiological and biochemical functions of the strain were completed using the detection kits API 50CH and API ZYM produced by bioMérieux France, and the detection system GEN III produced by BiOLOG USA. Other physiological characteristics of the strain, including Gram staining property, motility, oxygen requirement, catalase activity, gelatin liquefaction, indole production, H2S production, etc., were mainly referred to "Actinomycete System Identification Manual" (Xu LH. Actinomycete systematics: principles, methods and practices [M]. Beijing: Science Press, 2007, 93-108.).

[0066] The identification results showed that the strain G22706 was a Gram-positive aerobic heterotrophic bacterium. The cells were short rod-shaped with a size of (0.2-0.3) × (0.5-1) μm, without spore formation and non-motile. After the strain G22706 was cultured on PYG medium at 28 °C for 5 days, circular, convex, and light orange colonies with a diameter of about (1.5-3) mm were formed, and no soluble pigment was produced. See Figure 1。The growth tolerance range of the strain is 15 - 37 °C, 0 - 10% NaCl, pH 4.0 - 10.0, and the optimal growth conditions are 28 - 37 °C, 0% NaCl, pH 7.0 - 8.0. It is catalase - positive, gelatin - liquefaction - negative, and does not produce indole and H2S. The production of alkaline phosphatase, esterase C4, lipase C14, leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, chymotrypsin, α - galactosidase, β - galactosidase, β - glucuronidase, α - glucosidase and β - glucosidase is positive. The strain G22706 and its closest relative strain Gordonia bronchialis DSM 43247 T have the differences in physiological and biochemical characteristics shown in Table 1.

[0067] Table 1. Physiological and biochemical characteristics differences between strain G22706 and its relative strain DSM 43247 T Table of differences in physiological and biochemical characteristics

[0068]

[0069]

[0070]

[0071]

[0072] Note: +, positive / growth; -, negative / non - growth; W, weakly positive.

[0073] As can be seen from the results shown in Table 1, the strain G22706 of the present invention has differences in physiological and biochemical characteristics from the reported relative strains.

[0074] 2. Detection of cytochemical characteristics of strain G22706

[0075] The respiratory quinone types, polar lipids, fatty acids and other cytochemical components of strain G22706 were detected by HPLC liquid chromatography, TLC thin layer chromatography and GC gas chromatography techniques (Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233-241. Sasser M. Identification of bacteria by gas ghromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI inc; 1990.).

[0076] The results showed that in the strain G22706 of the present invention, MK-9(H2) was the main respiratory quinone type in the respiratory chain; diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylinositol were the main polar lipid components. Comparing the fatty acid components of the strain G22706 of the present invention with those of the related strain Gordonia bronchialis DSM 43247 T is shown in Table 2. It can be seen that the main fatty acid of the strain G22706 of the present invention is Summed Feature 4 (C 15:0 iso 2-OH / trans-C 16:1 ω9c), accounting for 33.3% of the total content, and the main fatty acid component of the closest related strain Gordonia bronchialis DSM43247 T is C 16:0 , accounting for 20.6% of the total content. At the same time, as shown in Table 2, the contents of many other fatty acid components in the strain G22706 are also different from those of its related strains. Therefore, the strain G22706 of the present invention is a new species of the genus Gordonia.

[0077] Table 2. Fatty acid composition table (%) of strain G22706 and its related reference strains

[0078]

[0079]

[0080] Note: Summed Feature 4,C 15:0 iso 2-OH / trans-C 16:1 ω9c; Summed Feature 8,cis-C 19:1 ω10c; -, not detected.

[0081] 3. Detection of cellulase activity of strain G22706

[0082] Using sodium carboxymethyl cellulose (CMC-Na) as the sole C source in the plate for plate screening to detect the cellulase activity of strain G22706 (Reinhold-Hurek, B., Hurek. T., Claeyssens, M., van Montagu, M. (1993). Cloning, expression in Escherichia coli, and characterization of cellulolytic enzymes of Azoarcus sp., a root-invading diazotroph. J Bacteriol 175, 7056 - 7065.). The specific steps are as follows: Prepare the CMC-Na screening medium (formula: (NH4)2SO4 4 g, NaCl 0.1 g, MgSO4·7H20 0.1 g, CaCl2 0.1 g, yeast extract 0.5 g, Fe(Ⅲ)EDTA 0.033 g, CMC-Na 2 g, agar 15 g, 1 L ddH2O, pH 7.0). Pick a single colony of strain G22706 in the logarithmic growth phase and inoculate it on the CMC-Na screening medium. After culturing for 3 days, use Congo red staining solution to detect the cellulose activity of the strain. The results are as Figure 2 shown. An obvious transparent circle was produced around strain G22706, indicating that strain G22706 has cellulolytic activity. The same experimental method was used for rescreening verification and the same experimental results as the primary screening were found (Teather, R.M., Wood, P.J. (1982). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43, 777 - 780.).

[0083] 4. Determination of the phylogenetic position of strain G22706

[0084] The genomic DNA of the strain G22706 of the present invention was extracted for sequencing, and the 16S rRNA gene sequence (SEQ ID No.1) was aligned online in the internationally authoritative bacterial taxonomy analysis database (http: / / www.ezbiocloud.net / ) (Kim OS, Cho YJ, Lee K, et al. 2012, Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol, 62:716-721). The results showed that the 16S rRNA gene sequence of the strain G22706 of the present invention had the highest similarity with the species of the genus Gordonia, and the representative strain of the species with the highest pairwise sequence similarity was Gordonia bronchialis DSM 43247 T , with a similarity of 98.4%, which is lower than the defined threshold of 98.7% for different bacterial species. It was preliminarily inferred that this bacterium was a new species of the genus Gordonia. The 16S rRNA gene sequences of the representative strains of the validly published species of the genus Gordonia were selected to construct a phylogenetic tree ( Figure 3 ).

[0085] To further clarify the phylogenetic status of the strain G22706, the average nucleotide identity (ANI value) of the whole genome sequence of the strain G22706 and the whole genome sequence of the closely related control bacterium was calculated and compared on EZbiocloud. The results showed that the ANI value of the strain G22706 and the closely related control bacterium Gordonia bronchialis DSM 43247 T was 76.9%, which was significantly lower than the ANI defined value (95%) for dividing two gene species (Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281-1286.). Combining the physiological and biochemical as well as chemical characteristics, the strain G22706 of the present invention was determined to be a new species of the genus Gordonia.

[0086] In summary, there are many significant differences between the strain G22706 of the present invention and the existing Gordonia strains in terms of phenotypic, physiological and biochemical, and cytochemical characteristics. At the same time, the phylogenetic analysis at the gene level further illustrates the differences between this strain and the existing published Gordonia species, fully demonstrating that the strain G22706 of the present invention represents a new species of the genus Gordonia. Meanwhile, through the detection of cellulose degradation ability, it is also proved that the strain of the present invention has cellulose degradation activity and is a functional new species of Gordonia, named Gordonia cellulolyticum. This bacterium has broad application prospects in food processing, garbage treatment, soil remediation, soil improvement, etc.

[0087] Gordonia cellulolyticum G22706 has been deposited with the China General Microbiological Culture Collection Center on July 7, 2022, and its deposit number is CGMCC No. 25246.

[0088] The present invention has been described in detail above. For those skilled in the art, without departing from the gist and scope of the present invention and without unnecessary experiments, the present invention can be implemented within a relatively wide range under equivalent parameters, concentrations and conditions. Although specific embodiments of the present invention are given, it should be understood that the present invention can be further improved. In short, according to the principle of the present invention, this application intends to cover any modifications, uses or improvements of the present invention, including those that depart from the scope disclosed in this application and are made by conventional techniques known in the art. Some basic features can be applied according to the scope of the appended claims below.

Claims

1. Gordonia fibrolytica ( Gordonia cellulolyticum ) G22706, with the registration number of CGMCC No. 25246 at the China General Microbiological Culture Collection Center.

2. A culture of Gordonia fibrolytica ([ Gordonia cellulolyticum Gordonia cellulolyticum ) G22706, which is a substance obtained by culturing Gordonia fibrolytica ([ Gordonia cellulolyticum Gordonia cellulolyticum ) G22706 in a bacterial culture medium.

3. Bacterial agent, characterized in that: The microbial agent contains the Gordonia fibrolytica ( Gordonia cellulolyticum ) G22706 described in claim 1 and / or the culture described in claim 2.

4. The microbial agent according to claim 3, characterized in that: The bacterial agent is a bacterial agent for hydrolyzing cellulose.

5. Use of Gordonia fibriata Gordonia cellulolyticum G22706 as claimed in claim 1, or the culture as claimed in claim 2, or the bacterial agent as claimed in claim 3 or 4, in any of the following: Gordonia cellulolyticum ​ (A1) Hydrolyze cellulose; (A2) Prepare a product for hydrolyzing cellulose; (A3) Prepare cellulase; (A4) Prepare a product with cellulase activity.

6. Use of Gordonia fibriata Gordonia cellulolyticum G22706 according to claim 1, or the culture according to claim 2, or the bacterial agent according to claim 3 or 4, in any of the following: Gordonia cellulolyticum ​ (B1) Food processing; (B2) Garbage treatment; (B3) Soil remediation; (B4) Soil improvement.

7. A product for hydrolyzing cellulose, the active ingredient of which is Gordonia fibrolytica ( Gordonia cellulolyticum ) G22706 described in claim 1, or the culture described in claim 2, or the bacterial agent described in claim 3 or 4.

8. A product with cellulase activity, the active ingredient of which is Gordonia fibrolytica ( Gordonia cellulolyticum ) G22706 described in claim 1, or the culture described in claim 2, or the bacterial agent described in claim 3 or 4.

9. A method for hydrolyzing cellulose, comprising the following steps: treating a sample to be treated with the Gordonia fibrolytica Gordonia cellulolyticum G22706 described in claim 1, or the culture described in claim 2, or the bacterial agent described in claim 3 or 4.

10. Use of Gordonia fibriata Gordonia cellulolyticum G22706 as claimed in claim 1 for preparing the culture as claimed in claim 2 or the microbial agent as claimed in claim 3 or 4. Gordonia cellulolyticum ​

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