A feeding method for obtaining selenium-rich mutton

Abstract

The application relates to the technical field of breeding, and discloses a feeding method for obtaining selenium-rich mutton, which comprises roughage and concentrate supplement, the roughage comprises selenium-rich alfalfa and tea stems, the concentrate supplement comprises selenium-rich corn, soybean meal, salt, selenium-rich additives and compound premix feed, the roughage is treated by cellulase and probiotics, and the compound premix feed comprises compound vitamins and trace elements. The above technical scheme solves the problems of low safety of selenium-rich sheep feed and low selenium content in selenium-rich mutton in the prior art.

Description

Technical Field

[0001] This invention relates to the field of aquaculture technology, specifically to a feeding method for obtaining selenium-enriched mutton. Background Technology

[0002] Selenium is an essential component of glutathione peroxidase and is recognized by the World Health Organization as an essential trace element for animal life. The physiological functions of selenium, its relationship with cardiovascular disease and immune function, and its anti-cancer mechanisms have been emphasized and studied in depth by researchers, and selenium-enriched foods are very popular among the general public.

[0003] Due to the lack of selenium in the soil, the food chain is generally low in selenium, leading to selenium deficiency or severe selenium deficiency in the human body. As a result, the development and utilization of selenium resources and the production of selenium-enriched agricultural and livestock products and health foods have gradually become new hot spots in related industries in my country.

[0004] Lamb is one of the main meats consumed by the general public. Lamb is more tender than pork and contains less fat and cholesterol than both pork and beef. Compared to pork, lamb has a higher protein content, lower fat content, and is rich in vitamins B1, B2, and B6, as well as iron and zinc. Furthermore, lamb is tender, easily digested and absorbed, and consuming more lamb helps improve the body's immunity. Generally, lamb is low in selenium. Researching selenium-enriched lamb products can greatly improve lamb quality (delicious flavor, lingering aftertaste, etc.), increase its added value, and enhance the economic value of sheep. It also helps people supplement their selenium intake through lamb, thus addressing insufficient selenium intake. This contributes to balanced nutrition, strong physique, and improved national health. Therefore, researching the production of selenium-enriched lamb not only creates high added value and improves breeding efficiency but also benefits people's livelihoods and generates positive social benefits, possessing significant research value and industrialization and promotion significance.

[0005] Currently, in order to increase the selenium content in mutton and obtain selenium-enriched mutton, there is an urgent need to develop a safe selenium-enriched sheep feed with high selenium utilization rate. Summary of the Invention

[0006] This invention proposes a feeding method for obtaining selenium-enriched mutton, which solves the problems of low safety of selenium-enriched sheep feed and low selenium content in selenium-enriched mutton in related technologies.

[0007] A selenium-enriched sheep feed includes roughage and concentrate supplements. The roughage includes selenium-enriched alfalfa and tea stalks. The concentrate supplements include selenium-enriched corn, soybean meal, salt, selenium-enriched additives, and compound premixed feed.

[0008] The roughage is treated with cellulase and probiotics;

[0009] The compound premixed feed includes compound vitamins and trace elements.

[0010] As a further technical solution, the probiotics are Clostridium butyricum and Leuconostoc mesenteroides.

[0011] As a further technical solution, the mass ratio of Clostridium butyricum and Leuconostoc mesenteroides is 1:2.

[0012] As a further technical solution, the concentrate supplement is 45-60% of the feed mass.

[0013] As a further technical solution, the feed comprises the following components by weight percentage: 30-35% selenium-enriched alfalfa, 10-15% tea stems, 33.2-38.2% selenium-enriched corn, 12.3-17.3% soybean meal, 0.2-0.3% salt, 0.3-0.4% selenium-enriched additives, and 4-5% compound premixed feed.

[0014] A method for preparing selenium-enriched sheep feed includes the following steps:

[0015] S1. Separately crush selenium-rich alfalfa and tea stems, then mix them to obtain mixture A;

[0016] S2. Add water to mixture A and stir to obtain mixture B;

[0017] S3. Add cellulase to mixture B, hydrolyze at 45-55℃ for 30 minutes, then sterilize to obtain the hydrolysate;

[0018] S4. Add probiotics to the enzymatic hydrolysate, and dry it after fermentation to obtain mixture C;

[0019] S5. After crushing selenium-enriched corn and soybean meal, mix them evenly with salt, selenium-enriched additives, and compound premixed feed to obtain mixture D;

[0020] S6, mixture C, and mixture D are mixed evenly, then stirred for another 6-9 minutes to obtain the feed;

[0021] In step S2, the amount of water added is 10-14% of the mass of mixture A.

[0022] As a further technical solution, the particle size of the pulverized selenium-enriched alfalfa and the pulverized tea stems are both 5.0-12.0 mm.

[0023] As a further technical solution, the amount of cellulase added in step S3 is 0.1%-0.2% of the mass of mixture B.

[0024] As a further technical solution, the amount of probiotics added in step S4 is 0.03-0.06 g / L.

[0025] As a further technical solution, the fermentation temperature in step S4 is 33-36℃, and the fermentation time is 48-52h.

[0026] An application of a selenium-enriched sheep feed, wherein the application is for feeding sheep to obtain selenium-enriched mutton.

[0027] As a further technical solution, the feeding method for obtaining selenium-enriched mutton includes the following steps:

[0028] SS1. Reasonable grouping: Divide the sheep into groups, with each sheep occupying 1m of sheepfold. 2 Each sheep occupies 3.5-4m of exercise space. 2 ;

[0029] SS2. Feeding: Before feeding, the amount of leftover feed in the trough should be 3±0.1%. Feed twice a day, once in the morning and once in the evening, with an interval of more than 8 hours between feedings. The amount of feed given each time should be half of the total amount of feed given that day, and the feeding time in the trough should be less than 3 hours each time.

[0030] SS3, Sheepfold Management: The relative humidity of the sheepfold should be 50-75%, and the temperature should be controlled between 14-22℃; vaccinate according to the disease prevention procedures and administer deworming drugs regularly; clean the sheepfold daily; disinfect and sterilize the sheepfold with disinfectant weekly, alternating disinfectants; disinfect every three days when there is an outbreak in the surrounding area.

[0031] The breeds of sheep in step SS1 include lambs and feeder sheep.

[0032] As a further technical solution, the disinfectant includes povidone-iodine, glutaraldehyde, benzalkonium chloride, and quicklime; the anthelmintic includes albendazole and ivermectin.

[0033] As a further technical solution, the relative humidity of the sheepfold in step SS3 is 50-60%.

[0034] The working principle and beneficial effects of this invention are as follows:

[0035] 1. This invention establishes a feed formula and feeding method for producing selenium-enriched mutton that is accurate, convenient, low-cost, safe to eat, and scientifically standardized. The selenium content in the selenium-enriched sheep feed is 0.41-0.45 mg / kg, and after feeding, the selenium content in the mutton is 0.27-0.31 mg / kg, meeting the "Classification Standard for Selenium Content in Selenium-Enriched Foods". It improves the brightness of the meat color, increases the content of flavor amino acids such as glutamic acid in the mutton, and improves the taste of the meat. Each selenium-enriched sheep generates 80-100 yuan more profit than ordinary sheep, thus improving economic efficiency.

[0036] 2. This invention pretreats selenium-enriched alfalfa and tea stalks in roughage by adding cellulase and probiotics, which can improve the absorption of various nutrients and elements in selenium-enriched alfalfa and tea stalks by sheep, and increase the selenium content in mutton. The probiotics are Clostridium butyricum and Leuconostoc mesenteroides. Clostridium butyricum promotes the proliferation of beneficial intestinal bacteria, regulates the balance of intestinal flora, promotes sheep growth, and has the ability to tolerate adverse environments. Leuconostoc mesenteroides can secrete bacteriocins to inhibit the growth of pathogenic bacteria, enabling sheep to grow healthily. Leuconostoc mesenteroides grows and reproduces rapidly with a short metabolic cycle, and can be quickly started in the early stage of fermentation, shortening the time for pretreating roughage and reducing production costs. Detailed Implementation

[0037] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0038] Examples 1-5 show that the selenium-enriched additives were prepared by uniformly mixing additive A (selenium-enriched yeast, selenate polysaccharide, nano-selenium), additive B (sodium selenite, sodium selenate) and carrier starch. The net selenium content in additive A was 0.003%, and the net selenium content in additive B was 0.003%. The mass ratio of additive A, additive B, and starch was 1:1:98.

[0039] Compound premixed feed: purchased from Rongda Feed Mill in Gaocheng City

[0040] Example 1

[0041] Sixty-two healthy, well-developed, and similarly aged hybrid lambs (from the first generation of crossbreeding between Hanbo sheep and Xiaowei Han sheep) of Yichen Livestock Co., Ltd. in Gaocheng District, Shijiazhuang City, Hebei Province, around 4 months old, were selected for the experiment. Before the experiment, the lambs were given a medicated bath, fed albendazole and ivermectin powder for deworming, and the sheepfold, feed troughs, and water troughs were disinfected to ensure that the lambs had free access to water.

[0042] The preparation method of selenium-enriched sheep feed includes the following steps:

[0043] S1. Weigh out the selenium-enriched sheep feed according to Table 1 and set aside.

[0044] S2. Selenium-enriched alfalfa and tea stems are crushed separately and then mixed to obtain mixture A, with a particle size of 7 mm.

[0045] S3. Add water to mixture A and stir to obtain mixture B; the amount of water added is 12% of the total amount of mixture A.

[0046] S4. Add cellulase to mixture B, hydrolyze at 50°C for 30 minutes, then sterilize to obtain the hydrolysate; the amount of cellulase added is 0.1% of the mass of mixture B.

[0047] S5. Add 0.01 g / L Clostridium butyricum and 0.02 g / L Leuconostoc mesenteroides to the enzymatic hydrolysate, ferment at 35°C for 50 h, and then dry to obtain mixture C;

[0048] S6. After crushing selenium-enriched corn and soybean meal to 2mm, mix them evenly with salt, selenium-enriched additives, and compound premixed feed to obtain mixture D.

[0049] S7, mixture C, and mixture D are mixed evenly, then stirred for another 6 minutes to obtain the feed;

[0050] The feeding method for obtaining selenium-enriched mutton includes the following steps:

[0051] SS1. Reasonable grouping: Divide the sheep into groups, with each sheep occupying 1m of sheepfold. 2 Each sheep occupies 4m of exercise space. 2 ;

[0052] SS2. Feeding: Clean up any leftover feed before feeding. The amount of leftover feed in the feed trough before feeding should be 3±0.1%. Feed twice a day, once in the morning and once in the evening. Feed at 7:30 am and 4:30 pm every day. The amount of feed given each time is half of the total amount of feed given that day. Each feeding time in the trough is 3 hours.

[0053] SS3, Sheepfold Management: Control the relative humidity of the sheepfold to 55±0.5% and the temperature to 20±0.2℃; vaccinate according to the disease prevention procedures and administer deworming drugs regularly; clean and tidy up the fences, walls, ceilings, feed troughs, water troughs, manure storage tanks, and other breeding facilities and equipment daily; disinfect and sterilize the entire area with disinfectant weekly, alternating disinfectants; disinfect every three days when there is an outbreak in the surrounding area; disinfectants include povidone-iodine, glutaraldehyde, benzalkonium chloride, and quicklime; deworming drugs include albendazole and ivermectin.

[0054] Table 1. Proportion of each component in selenium-enriched sheep feed (%)

[0055]

[0056] The selenium content in the selenium-enriched sheep feed shown in Table 1 is 0.45 mg / kg.

[0057] The sheep were fed a diet for 73 days. Their health and weight were observed and recorded daily, and the selenium content in the mutton was measured.

[0058] Determination of selenium content in mutton: Refer to GB 5009.93-2017 "National Food Safety Standard - Determination of Selenium in Food".

[0059] Table 2 Measurement Results

[0060]

[0061]

[0062] Example 2: Experiment on the effect of selenium-enriched feed ingredients on the production of selenium-enriched mutton from lambs.

[0063] Sixty-two healthy, well-developed, and similarly aged crossbred Hanbo sheep and Xiaowei Han sheep (generation 1) from Haishengwo Livestock Co., Ltd., Gaocheng District, Shijiazhuang City, Hebei Province, were selected and weaned at 2.5 months of age. They were divided into two groups of 32 sheep each: an experimental group and a control group. Before the experiment, the experimental sheep were given a medicated bath and dewormed, and the sheepfold, feed troughs, and water troughs were disinfected. All 62 experimental sheep were housed together in the same trough, with free access to water.

[0064] 1) Experimental group 2

[0065] The preparation method of selenium-enriched sheep feed includes the following steps:

[0066] S1. Weigh out the selenium-enriched sheep feed according to Table 3 and set aside.

[0067] S2. Selenium-enriched alfalfa and tea stems are crushed separately and then mixed to obtain mixture A, with a particle size of 5 mm.

[0068] S3. Add water to mixture A and stir to obtain mixture B; the amount of water added is 10% of the total amount of mixture added.

[0069] S4. Add cellulase to mixture B, enzymatically hydrolyze at 45℃ for 30 min, then sterilize to obtain the enzymatic hydrolysate; the amount of cellulase added is 0.2% of the mass of mixture B.

[0070] S5. Add 0.02 g / L Clostridium butyricum and 0.04 g / L Leuconostoc mesenteroides to the enzymatic hydrolysate, ferment at 33°C for 52 h, and then dry to obtain mixture C;

[0071] S6. After crushing selenium-enriched corn and soybean meal to 1mm, mix them evenly with salt, selenium-enriched additives, and compound premixed feed to obtain mixture D.

[0072] S7, mixture C, and mixture D are mixed evenly, and then stirred for another 9 minutes to obtain the feed.

[0073] The feeding method for obtaining selenium-enriched mutton includes the following steps:

[0074] SS1. Reasonable grouping: Divide the sheep into groups, with each sheep occupying 1m of sheepfold. 2 Each sheep occupies 3.5m of exercise area. 2 ;

[0075] SS2. Feeding: Clean up any leftover feed before feeding. The amount of leftover feed in the feed trough before feeding should be 3±0.1%. Feed twice a day, once in the morning and once in the evening. Feed at 7:00 am and 5:00 pm every day. The amount of feed given each time is half of the total amount of feed given that day. Each feeding time is 2.5 hours.

[0076] SS3, Sheepfold Management: Control the relative humidity of the sheepfold to 50±0.5% and the temperature to 14±0.2℃; vaccinate according to the disease prevention procedures and administer deworming drugs regularly; clean and tidy up the fences, walls, ceilings, feed troughs, water troughs, manure storage tanks, and other breeding facilities and equipment daily; disinfect and sterilize the entire area with disinfectant weekly, alternating disinfectants; disinfect every three days when there is an outbreak in the surrounding area; disinfectants include povidone-iodine, glutaraldehyde, benzalkonium chloride, and quicklime; deworming drugs include albendazole and ivermectin.

[0077] 2) Control group 2

[0078] The method for preparing sheep feed includes the following steps:

[0079] S1. Weigh each component from control group 2 according to Table 3 and set aside.

[0080] S2. After crushing ordinary hay, silage corn stalks, and ordinary corn to 1mm, a mixture is obtained;

[0081] S3. Mix the mixture with the remaining components to obtain sheep feed.

[0082] The sheep were fed according to the feeding method used to obtain selenium-enriched mutton in experimental group 2.

[0083] Table 3. Proportions (%) of each component in the selenium-enriched sheep feed for experimental group 2 and control group 2

[0084]

[0085] In Table 2, the selenium content in the selenium-enriched sheep feed of experimental group 2 was 0.41 mg / kg; the selenium content in the sheep feed of control group 2 was 0.16 mg / kg.

[0086] The pre-feeding period is 3 days, and the formal feeding period is 36 days. The health of the sheep is observed and recorded every day, and the selenium content in the mutton is measured.

[0087] Table 4. Selenium content in mutton in experimental group 2 and control group 2

[0088]

[0089] Control group 2 was fed a standard diet. As shown in Table 4, the selenium content in the mutton of experimental group 2 was higher than that of control group 2. Compared with Example 1, experimental group 2 replaced 5% by weight of selenium-enriched corn with 5% by weight of selenium-enriched alfalfa. The selenium content in the feed of experimental group 2 was lower than that of Example 1, but the selenium content in the mutton of experimental group 2 was higher than that of Example 1. This indicates that although selenium-enriched corn has a high selenium content, the absorption and utilization rate of selenium is lower than that of selenium-enriched alfalfa.

[0090] Example 3: Experiment on the effect of selenium-enriched feed ingredients on the production of selenium-enriched mutton from feeder sheep.

[0091] Sixty-eight healthy, well-developed, and similarly aged hybrid lambs (F1 generation) of Hanbo and Xiaowei Han sheep from Haishengwo Livestock Co., Ltd., Gaocheng District, Shijiazhuang City, Hebei Province, were selected and divided into two groups of 34 lambs each: an experimental group and a control group. Before the experiment, all 68 experimental lambs underwent medicated baths and were given deworming medication. The sheepfold, feed troughs, and water troughs were disinfected. The experimental lambs were housed together in the same troughs, and free access to water was ensured for all lambs.

[0092] 1) Experimental group 3

[0093] The preparation method of selenium-enriched sheep feed includes the following steps:

[0094] S1. Weigh out the selenium-enriched sheep feed according to Table 3 and set aside.

[0095] S2. Selenium-enriched alfalfa and tea stems are crushed separately and then mixed to obtain mixture A, with a particle size of 9 mm.

[0096] S3. Add water to mixture A and stir to obtain mixture B; the amount of water added is 14% of the total amount of mixture added.

[0097] S4. Add cellulase to mixture B, enzymatically hydrolyze at 55℃ for 30 min, then sterilize to obtain the enzymatic hydrolysate; the amount of cellulase added is 0.15% of the mass of mixture B.

[0098] S5. Add 0.015 g / L Clostridium butyricum and 0.03 g / L Leuconostoc mesenteroides to the enzymatic hydrolysate, ferment at 36°C for 48 h, and then dry to obtain mixture C;

[0099] S6. After crushing selenium-enriched corn and soybean meal to 3mm, mix them evenly with salt, selenium-enriched additives, and compound premixed feed to obtain mixture D.

[0100] S7, mixture C, and mixture D are mixed evenly, then stirred for another 8 minutes to obtain the feed.

[0101] The feeding method for obtaining selenium-enriched mutton includes the following steps:

[0102] SS1. Reasonable grouping: Divide the sheep into groups, with each sheep occupying 1m of sheepfold. 2 Each sheep occupies 4m of exercise space. 2 ;

[0103] SS2. Feeding: Clean up any leftover feed before feeding. The amount of leftover feed in the feed trough before feeding should be 3±0.1%. Feed twice a day, once in the morning and once in the evening. Feed at 7:00 am and 5:00 pm every day. The amount of feed given each time is half of the total amount of feed given that day. Each feeding time in the trough is 3 hours.

[0104] SS3, Sheepfold Management: Control the relative humidity of the sheepfold to 60±0.5% and the temperature to 22±0.2℃; vaccinate according to the disease prevention procedures and administer deworming drugs regularly; clean and tidy up the fences, walls, ceilings, feed troughs, water troughs, manure storage tanks, and other breeding facilities and equipment daily; thoroughly disinfect and sterilize with disinfectant weekly, alternating disinfectants; disinfect every three days when there is an outbreak in the surrounding area; disinfectants include povidone-iodine, glutaraldehyde, benzalkonium chloride, and quicklime; deworming drugs include albendazole and ivermectin.

[0105] 2) Control group 3

[0106] Feed preparation method: Compared with control group 2 in Example 2, control group 3 weighed each component of the feed according to Table 5 and set aside. Other aspects were the same as control group 2.

[0107] Feeding method: The feeding method is the same as that of experimental group 3 above.

[0108] Table 5. Proportions of each component in selenium-enriched sheep feed (%)

[0109]

[0110] In Table 5, the selenium content in the selenium-enriched sheep feed of experimental group 3 was 0.45 mg / kg; the selenium content in the sheep feed of control group 3 was 0.15 mg / kg.

[0111] The pre-feeding period is 5 days, and the formal feeding period is 76 days. The health of the sheep is observed and recorded every day, and the selenium content in the mutton is measured.

[0112] Table 6 Selenium content in mutton

[0113]

[0114] The control group 3 was fed with ordinary feed. As shown in Table 6, the selenium content of the mutton in the experimental group 3 was higher than that in the control group 3.

[0115] Example 4

[0116] Sixty healthy, well-developed, and similarly aged crossbred Hanbo sheep and Xiaowei Han sheep (generation 1) from Haishengwo Livestock Co., Ltd., Gaocheng District, Shijiazhuang City, Hebei Province, were selected and weaned at 2.5 months of age. They were divided into two groups of 30 sheep each: an experimental group and a control group. Before the experiment, the experimental sheep underwent medicated baths and were given deworming medication. The sheepfold, feed troughs, and water troughs were disinfected. The experimental sheep were housed in the same trough within the same group, and the young sheep had free access to water.

[0117] Compared with Example 1, in the preparation method of selenium-enriched sheep feed in Experiment Group 4, step S1 is to weigh the selenium-enriched sheep feed according to Table 7, and the rest is the same as in Example 1;

[0118] Experimental group 4 was fed according to the feeding method for the selenium-enriched mutton obtained in Example 1.

[0119] Table 7. Proportion of each component in selenium-enriched sheep feed (%)

[0120]

[0121] In Table 7, the selenium content in the selenium-enriched sheep feed of experimental group 4 was 0.41 mg / kg. The sheep were fed for 33 days, and their health was observed and recorded daily. The selenium content in the mutton was also measured.

[0122] Table 8 Selenium content in mutton

[0123]

[0124] Example 5: Experiment on the effect of feeding duration on the production of selenium-enriched mutton from feeder sheep

[0125] Thirty healthy, well-developed, and similarly aged crossbred Hanbo sheep and Xiaowei Han sheep (generation 1) from Haishengwo Livestock Co., Ltd., Gaocheng District, Shijiazhuang City, Hebei Province, were selected to determine the selenium content in the lamb meat at different feeding durations. Before the experiment, the sheep were given medicated baths and deworming medication, and the sheepfold, feed troughs, and water troughs were disinfected. The sheep were housed together in the same trough, and free access to water was ensured for all lambs.

[0126] Compared with Example 1, in the preparation method of selenium-enriched sheep feed in Example 5, step S1 is to weigh the selenium-enriched sheep feed according to Table 9 and set it aside, while the rest is the same as in Example 1.

[0127] The sheep were fed according to the feeding method for obtaining selenium-enriched mutton in Example 1.

[0128] Table 9. Proportion of each component in selenium-enriched sheep feed (%)

[0129]

[0130] The selenium content in the selenium-enriched sheep feed shown in Table 9 is 0.41 mg / kg.

[0131] The sheep were fed for 43 days. The health of the sheep was observed and recorded every day. The selenium content of the mutton was measured on the 5th, 10th, 15th, 20th, 30th and 35th days of feeding. The results are shown in Table 10.

[0132] Table 10 Selenium content in mutton

[0133]

[0134] As shown in Table 10, the highest level of selenium enrichment in mutton was achieved after 20 days of feeding.

[0135] Comparative Example 1

[0136] Compared with Example 1, the roughage in Comparative Example 1 was not pretreated with cellulase, Clostridium butyricum, and Leuconostoc mesenteroides, but otherwise it was the same as in Example 1.

[0137] Comparative Example 2

[0138] Compared with Example 1, the roughage in Comparative Example 2 was not treated with cellulase, but otherwise it was the same as in Example 1.

[0139] Comparative Example 3

[0140] Compared with Example 1, the roughage of Comparative Example 3 was not treated with Clostridium butyricum and Leuconostoc mesenteroides, but otherwise it was the same as that of Example 1.

[0141] Comparative Example 4

[0142] Compared with Example 1, Comparative Example 4 replaced Clostridium butyricum with an equal amount of Leuconostoc mesenteroides, and was otherwise the same as Example 1.

[0143] Comparative Example 5

[0144] Compared with Example 1, Comparative Example 5 replaced Leuconostoc mesenteroides with an equal amount of Clostridium butyricum, and was otherwise the same as Example 1.

[0145] Comparative Example 6

[0146] Compared with Example 1, Comparative Example 6 replaced "0.01g / L Clostridium butyricum and 0.02g / L Leuconostoc mesenteroides" in step S2 with "0.02g / L Clostridium butyricum and 0.01g / L Leuconostoc mesenteroides", and the rest was the same as in Example 1.

[0147] Comparative Example 7

[0148] Compared with Example 1, Comparative Example 7 replaced Clostridium butyricum with an equal amount of Bacillus subtilis, and was otherwise the same as Example 1.

[0149] Table 13 Selenium content of lamb in Example 1 and Comparative Examples 1-7

[0150] Selenium content in mutton (mg / kg) Example 1 0.27 Comparative Example 1 0.1 Comparative Example 2 0.19 Comparative Example 3 0.16 Comparative Example 4 0.20 Comparative Example 5 0.22 Comparative Example 6 0.25 Comparative Example 7 0.19

[0151] Compared with Example 1, the roughage in Comparative Example 1 was not pretreated with cellulase and Clostridium butyricum and Leuconostoc mesenteroides, Comparative Example 2 was not treated with cellulase, and Comparative Example 3 was not treated with Clostridium butyricum and Leuconostoc mesenteroides. The selenium content of mutton in Comparative Examples 1-3 was lower than that in Example 1. Therefore, pretreatment with cellulase and Clostridium butyricum and Leuconostoc mesenteroides can promote the absorption of selenium by sheep, thereby increasing the selenium content in mutton.

[0152] Compared with Example 1, Comparative Example 4 replaced Clostridium butyricum with an equal amount of Leuconostoc mesenteroides, Comparative Example 5 replaced Leuconostoc mesenteroides with an equal amount of Clostridium butyricum, Comparative Example 6 changed the addition ratio of Clostridium butyricum and Leuconostoc mesenteroides, and Comparative Example 7 replaced Clostridium butyricum with an equal amount of Bacillus subtilis. As shown in Table 13, the selenium content of mutton in Comparative Examples 4-6 was lower than that in Example 1. Therefore, the selenium content of mutton was reduced by adding Clostridium butyricum alone or by adding Leuconostoc mesenteroides alone. Moreover, changing the addition ratio of Clostridium butyricum and Leuconostoc mesenteroides and replacing Clostridium butyricum with Bacillus subtilis also resulted in a lower selenium content in mutton than in Example 1.

[0153] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A selenium-enriched sheep feed, characterized in that, It includes roughage and concentrate supplements. The roughage includes selenium-enriched alfalfa and tea stalks. The concentrate supplements include selenium-enriched corn, soybean meal, salt, selenium-enriched additives, and compound premixed feed. The roughage is treated with cellulase and probiotics; The compound premixed feed includes compound vitamins and trace elements; The probiotics consist of Clostridium butyricum and Leuconostoc mesenteroides in a mass ratio of 1:

2.

2. The selenium-enriched sheep feed according to claim 1, characterized in that, The concentrate supplement constitutes 45-60% of the feed weight.

3. The selenium-enriched sheep feed according to claim 1, characterized in that, By weight percentage, the feed comprises the following components: 30-35% selenium-enriched alfalfa, 10-15% tea stalks, 33.2-38.2% selenium-enriched corn, 12.3-17.3% soybean meal, 0.2-0.3% salt, 0.3-0.4% selenium-enriched additives, and 4-5% compound premixed feed.

4. The method for preparing selenium-enriched sheep feed according to claim 1, characterized in that, Includes the following steps: S1. Separately crush selenium-rich alfalfa and tea stems, then mix them to obtain mixture A; S2. Add water to mixture A and stir to obtain mixture B; S3. Add cellulase to mixture B, hydrolyze at 45-55℃ for 30 minutes, then sterilize to obtain the hydrolysate; S4. Add probiotics to the enzymatic hydrolysate, and dry it after fermentation to obtain mixture C; S5. After crushing selenium-enriched corn and soybean meal, mix them evenly with salt, selenium-enriched additives, and compound premixed feed to obtain mixture D; S6, mixture C, and mixture D are mixed evenly, then stirred for another 6-9 minutes to obtain the feed; In step S2, the amount of water added is 10-14% of the mass of mixture A.

5. The method for preparing selenium-enriched sheep feed according to claim 4, characterized in that, In step S3, the amount of cellulase added is 0.1%-0.2% of the mass of mixture B.

6. The method for preparing selenium-enriched sheep feed according to claim 4, characterized in that, The amount of probiotics added in step S4 is 0.03-0.06 g / L.

7. The method for preparing selenium-enriched sheep feed according to claim 4, characterized in that, In step S4, the fermentation temperature is 33-36℃ and the fermentation time is 48-52h.

8. The application of a selenium-enriched sheep feed according to any one of claims 1-3, characterized in that, The application is for feeding sheep to obtain selenium-enriched mutton.

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