Mouse intestinal tumor cell injector

Abstract

The mouse intestinal tumor cell injector comprises a handle, an injection device installed in the handle, and a radially telescopic support ring structure connected with the handle, tumor cells can be discharged from a first injection needle by operating a first push rod, and antibiotics can be discharged from a second injection needle by operating a second push rod; when the support ring structure is in a contracted state, the first injection needle and the second injection needle are located in the support ring structure; when the support ring structure is in an expanded support state, the needle heads of the first injection needle and the second injection needle can both extend to the outside of the outer edge of the support ring structure and are inserted into the mouse intestinal wall. The present application supports the mouse intestine by the support ring structure, supports the insertion of the first injection needle and the second injection needle into the mouse intestinal wall, ensures that only the mouse intestinal endothelium is punctured and the mouse intestinal canal is not punctured, ensures that tumor cells are injected into the mouse intestinal wall, and when the tumor cells are injected, antibiotics are also injected for anti-inflammatory treatment, which is beneficial to the recovery of the mouse intestinal puncture point.

Description

Technical Field

[0001] This invention belongs to the field of mouse intestinal experiments, specifically relating to a syringe for mouse intestinal tumor cells. Background Technology

[0002] To induce colon or rectal cancer in mice for subsequent research, a suspension of rectal cancer cells needs to be injected into the mouse's colon or rectum. The current method involves anesthetizing the mice, then surgically opening the abdominal cavity to inject tumor cells into the intestinal wall. This method is time-consuming, labor-intensive, and does not reflect the actual tumor growth patterns in clinical patients.

[0003] CN111214752A discloses an intestinal submucosal injection device, which includes a control catheter and an expandable component, facilitating the insertion of the injection device, located on the outside of the expandable component, into a designated location in the intestine as needed. When this injection device is used to inject intestinal tumor cells into mice, because it can only inject one drug, it cannot perform anti-inflammatory treatment on the mouse intestine while injecting tumor cells; that is, it cannot inject antibiotics simultaneously with tumor cell injection, which is detrimental to the recovery of the intestinal puncture site in mice. Summary of the Invention

[0004] The present invention aims to solve the technical problems existing in the prior art, and the purpose of the present invention is to provide a mouse intestinal tumor cell injector.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a mouse intestinal tumor cell injector, comprising a hollow handle, an injection device installed in the handle, and a radially retractable support ring structure connected to the front end of the handle. The injection device has a first injection needle, a second injection needle, a first push rod, and a second push rod. By operating the first push rod, tumor cells can be discharged from the first injection needle, and by operating the second push rod, antibiotics can be discharged from the second injection needle. When the support ring structure is in a contracted state, the first and second injection needles are located within the support ring structure. When the support ring structure is in an extended supported state, the tips of both the first and second injection needles can extend beyond the outer edge of the support ring structure and insert into the mouse intestinal wall.

[0006] In the above-described technical solution, the support ring structure is normally in a contracted state, facilitating the insertion of the syringe into the mouse intestine. The support ring structure expands the mouse intestine, serving as a support for the insertion of the first and second injection needles into the intestinal wall. This ensures that only the intestinal endothelium is punctured, without perforating the intestinal tract, guaranteeing the injection of tumor cells into the intestinal wall. Furthermore, antibiotics are injected simultaneously with the tumor cells for anti-inflammatory treatment, promoting recovery of the puncture site in the mouse intestine.

[0007] In a preferred embodiment of the present invention, the injection device further includes a first injection cylinder and a second injection cylinder, a first piston and a second piston are slidably connected in the first injection cylinder and the second injection cylinder respectively, a first injection needle and a second injection needle are respectively connected to the outlets at the front ends of the first injection cylinder and the second injection cylinder, a first push rod and a second push rod are respectively fixedly connected to the first piston and the second piston, and a handle is sleeved outside the first injection cylinder and the second injection cylinder, and the first injection cylinder and the second injection cylinder are slidably connected to the handle through a groove on the handle.

[0008] In the above technical solution, the first and second syringes are used to hold tumor cells and antibiotic solution, respectively. By sliding the first and second syringes in the groove of the handle, the first and second injection needles can be inserted into the intestinal wall of the mouse. By pushing the first and second push rods, the tumor cells and antibiotic solution can be injected into the intestinal wall of the mouse. The operation is simple.

[0009] In a preferred embodiment of the present invention, the slide includes a longitudinal slide extending along the length direction of the handle and a transverse slide extending along the width direction of the handle; when the first injection cylinder and the second injection cylinder are slidably connected to the longitudinal slide, the first injection needle and the second injection needle can extend to the outer edge of the support ring structure or retract into the support ring structure; when the first injection cylinder and the second injection cylinder are slidably connected to the transverse slide, the first injection needle and the second injection needle can be inserted into the intestinal wall of the mouse.

[0010] The above technical solution, by setting longitudinal and transverse slide grooves, allows the first and second injection cylinders to slide forward in the longitudinal slide groove, thus pushing the first and second injection needles out of the support ring structure. By sliding left and right in the transverse slide groove, the first and second injection needles can be inserted into the mouse intestinal wall.

[0011] In a preferred embodiment of the present invention, the first injection cylinder and the second injection cylinder are further connected by a locking buckle. The first injection cylinder is elastically connected to a first slider through a first elastic unit, and the second injection cylinder is elastically connected to a second slider through a second elastic unit. The first slider and the second slider are slidably connected in corresponding longitudinal grooves. When the first injection cylinder and the second injection cylinder are locked by the locking buckle for self-locking, the first injection cylinder and the second injection cylinder can slide in the longitudinal groove. After the locking buckle is released, under the elastic force of the first elastic unit and the second elastic unit, the first injection cylinder and the second injection cylinder can slide in the corresponding transverse groove and insert the first injection needle and the second injection needle into the intestinal wall of the mouse.

[0012] The above technical solution, by setting a latch, a first elastic unit and a second elastic unit, in normal condition, the latch locks the first and second syringes, and the first and second elastic units store energy; after the first and second injection needles are pushed out of the support ring structure, the latch is released, and under the elastic force of the first and second elastic units, the first and second injection needles automatically insert into the mouse intestinal wall.

[0013] In a preferred embodiment of the present invention, the front end of the longitudinal groove has a front limiting block.

[0014] In the above technical solution, the front limit block limits the termination position of the first and second injection cylinders as they slide forward, ensuring that the first and second injection needles are outside the support ring structure and close to the support ring structure.

[0015] In another preferred embodiment of the present invention, the first injection cylinder and the second injection cylinder are both arranged parallel to the length direction of the handle, and the first injection cylinder and the second injection cylinder are respectively located on the left and right sides inside the handle and are staggered in the vertical direction.

[0016] In the above technical solution, the first and second syringes are spatially offset, and their left and right movements in the handle do not interfere with each other.

[0017] In another preferred embodiment of the present invention, a push block is fixedly connected to the rear end of the first push rod. When the first injection needle and the second injection needle are inserted into the intestinal wall of the mouse, the push block abuts against the rear end of the second push rod.

[0018] The above technical solution connects the first push rod and the second push rod by setting a push block, thereby enabling the simultaneous injection of tumor cells and antibiotic solution by pushing the push block forward.

[0019] In another preferred embodiment of the present invention, the first syringe contains tumor cells and petroleum jelly located behind the tumor cells, and the tumor cells and petroleum jelly are separated by a first septum slidably connected to the first syringe. The front end of the inside of the first syringe has a spike capable of piercing the first septum. The second syringe contains antibiotic solution and petroleum jelly located behind the antibiotic solution, and the antibiotic solution and petroleum jelly are separated by a second septum slidably connected to the second syringe. The front end of the inside of the second syringe also has a spike capable of piercing the second septum.

[0020] The above technical solution divides the first syringe into two chambers by setting a first diaphragm and the second syringe into two chambers by setting a second diaphragm. After the tumor cells and antibiotic solution are injected, the first and second diaphragms are punctured by a thorn, and petroleum jelly can be injected to seal the two puncture points, preventing the tumor cells and antibiotic solution in the intestinal wall from spilling out.

[0021] In another preferred embodiment of the present invention, a marking block is fixedly attached to the side wall of the first push rod / second push rod. When the first diaphragm / second diaphragm advances to the spike, the marking block is aligned with the rear end of the first syringe / second syringe. A locking block that can intermittently abut against the outer wall of the first syringe and the second syringe is movably connected to the inner wall of the handle.

[0022] The above technical solution uses marker blocks to remind operators that tumor cells and antibiotic solutions have been injected; and uses locking blocks to fix the left and right positions of the first and second syringes, facilitating subsequent injection of petroleum jelly.

[0023] In another preferred embodiment of the present invention, the support ring structure includes a ring body fixedly connected to the front end of the handle, and an airbag fixedly connected to the ring body and disposed around it. The airbag has an inflated support state and a deflated contracted state, and the airbag is connected to an inflation / deflation device through a pipeline.

[0024] The above technical solution uses the radial expansion and contraction of the support ring structure by inflating or deflating the air bladder. It is simple to operate, and the air bladder is soft and will not damage the mouse intestine.

[0025] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0026] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0027] Figure 1 This is a schematic diagram of the mouse intestinal tumor cell injector in its normal contracted state, as described in the embodiment.

[0028] Figure 2 It is Figure 1 The diagram shows the support ring structure in an unfolded support state.

[0029] Figure 3 It is Figure 2 A schematic diagram showing the first and second injection cylinders being pushed outside the support ring structure.

[0030] Figure 4 This is a schematic diagram showing the injection device in the injection state after the lock is released.

[0031] Figure 5 This is a schematic diagram showing the state of Vaseline being injected using the first injection device.

[0032] The reference numerals in the accompanying drawings include: handle 10, longitudinal slide groove 11, transverse slide groove 12, front limit stop 13, locking block 14, support ring structure 20, ring body 21, airbag 22, pipeline 23, first injection device 30, first injection needle 31, first push rod 32, first injection cylinder 33, first piston 34, first slider 35, first elastic unit 36, first diaphragm 37, push block 38, lock 39, second injection device 40, second injection needle 41, second push rod 42, second injection cylinder 43, second piston 44, second slider 45, second elastic unit 46, second diaphragm 47, spike 48, and marking block 49. Detailed Implementation

[0033] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0034] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "vertical", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0035] In the description of this invention, unless otherwise specified and limited, it should be noted that the terms "installation", "connection" and "linking" should be interpreted broadly. For example, they can refer to mechanical or electrical connections, or internal connections between two components. They can be direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0036] This invention provides a mouse intestinal tumor cell injector, such as... Figure 1 and Figure 4 As shown, in a preferred embodiment of the present invention, the syringe includes a hollow handle 10, an injection device mounted in the handle 10, and a radially retractable support ring structure 20 connected to the front end of the handle 10. The injection device has a first injection needle 31, a second injection needle 41, a first plunger 32, and a second plunger 42. By operating the first plunger 32, tumor cells can be discharged from the first injection needle 31, and by operating the second plunger 42, antibiotics can be discharged from the second injection needle 41.

[0037] like Figure 1 As shown, when the support ring structure 20 is in the contracted state, the first injection needle 31 and the second injection needle 41 are located within the support ring structure 20. Figure 4 As shown, when the support ring structure 20 is in the unfolded support state, the needles of the first injection needle 31 and the second injection needle 41 can both extend to the outer edge of the support ring structure 20 and insert into the mouse intestinal wall.

[0038] In this embodiment, the support ring structure 20 includes a ring body 21 fixedly connected to the front end of the handle 10, and an airbag 22 fixedly connected to and surrounding the ring body 21. The airbag 22 has an inflated support state and a deflated contracted state. The airbag 22 is connected to an inflation / deflation device (not shown in the figure) via a pipe 23. The inflation / deflation device is a miniature air pump or an inflatable airbag. The pipe 23 is connected to the inner wall of the ring body 21 in close contact with the airbag 22, and the pipe 23 extends through the inside of the handle 10 to the outer rear end of the handle 10.

[0039] Adopting such a technical solution, such as Figure 1 As shown, under normal conditions, the air sac 22 is in a deflated, contracted state, thus the support ring structure 20 is also in a contracted state. The first injection needle 31 and the second injection needle 41 are located within the support ring structure 20, facilitating the insertion of the syringe into the mouse intestine. The operator holds the handle 10 and inserts the support ring structure 20 into the mouse intestine (either the colon or rectum, depending on the situation) through the mouse anus. Next, the air sac 22 is inflated using the inflation / deflation device, placing it in an inflated, supported state. This causes the support ring structure 20 to unfold and become supported, opening the mouse intestine and fixing its position within the intestine, facilitating subsequent injection of tumor drugs. Next, as... Figure 4 As shown, the operator extends the first injection needle 31 and the second injection needle 41 outside the support ring structure 20 and close to the support ring structure 20. The tip of the first injection needle 31 is inserted into the mouse intestinal wall, and the second injection needle 41 is inserted into the mouse intestinal wall on the opposite side. Then, the tumor cells are injected into the mouse intestinal wall by operating the first push rod 32, and the antibiotics are injected into the mouse intestinal wall by operating the second push rod 42.

[0040] This invention uses a support ring structure 20 to open the mouse intestine. When the first injection needle 31 and the second injection needle 41 are inserted into the mouse intestinal wall, the support ring structure 20 plays a positioning role, ensuring that the first injection needle 31 and the second injection needle 41 only puncture the mouse intestinal endothelium and do not puncture the mouse intestinal tract. This ensures that tumor cells are injected into the mouse intestinal wall, which is more in line with the actual tumor growth situation of clinical patients.

[0041] In this embodiment, to prevent the extravasation of injected tumor cells and antibiotic solution, the first injection needle 31 and the second injection needle 41 can be retracted into the support ring structure 20 or the injection device can be removed from the handle 10. The support ring structure 20 can then be advanced to the puncture points of the first injection needle 31 and the second injection needle 41 and the two puncture points can be sealed. The support ring structure 20 is retained in the mouse intestine for 1-2 hours and then contracted and removed.

[0042] In this embodiment, to facilitate the operator in knowing the depth of the support ring structure 20 inserted into the mouse intestine, a scale line is provided on the outer wall of the handle 10.

[0043] In this invention, the injection device includes a first injection device 30 and a second injection device 40. Tumor cells are injected by the first injection device 30, and antibiotic solution is injected by the second injection device 40. The first injection device 30 also includes a first injection cylinder 33 located in the handle 10. A first piston 34 is slidably connected in the first injection cylinder 33. A first injection needle 31 is connected to the outlet at the front end of the first injection cylinder 33, and a first push rod 32 is fixedly connected to the rear end of the first piston 34. The second injection device 40 also includes a second injection cylinder 43 located in the handle 10. A second piston 44 is slidably connected in the second injection cylinder 43. A second injection needle 41 is connected to the outlet at the front end of the second injection cylinder 43, and a second push rod 42 is fixedly connected to the rear end of the second piston 44. Both the first injection cylinder 33 and the second injection cylinder 43 are slidably connected to the handle 10 via a groove on the handle 10.

[0044] Preferably, the first syringe 33 and the second syringe 43 are both arranged parallel to the length direction of the handle 10, and the first syringe 33 and the second syringe 43 are located on the left and right sides of the handle 10 respectively and are staggered in the vertical direction, for example... Figure 1 The second syringe 43 shown is located above and to the right of the first syringe 33, so that the left and right movements of the first injection device 30 and the second injection device 40 in the handle 10 do not interfere with each other. The first injection needle 31 bends to the right from the front end of the first syringe 33, and the second injection needle 41 bends to the left from the front end of the second syringe 43.

[0045] Adopting such a technical solution, such as Figure 2 As shown, when the support ring structure 20 is in an expanded support state and the mouse intestine is stretched open, the first injection needle 31 and the second injection needle 41 remain within the support ring structure 20. Then, as... Figure 4 As shown, the first injection cylinder 33 and the second injection cylinder 43 are slid in the groove of the handle 10 so that the needles of the first injection needle 31 and the second injection needle 41 extend to the outer edge of the support ring structure 20 and are inserted into the mouse intestinal wall.

[0046] In another preferred embodiment of the invention, such as Figure 1 , Figure 3 and Figure 4 As shown, the slide includes a longitudinal slide 11 extending along the length of the handle 10 and a transverse slide 12 extending along the width of the handle 10. Preferably, the longitudinal slide 11 extends forward to the ring body 21, and the front end of the longitudinal slide 11 has a front limiting block 13 that serves as a limiting stop. When the first injection cylinder 33 and the second injection cylinder 43 are slidably connected to the longitudinal slide 11, the first injection needle 31 and the second injection needle 41 can extend out to the outer edge of the support ring structure 20 or retract into the support ring structure 20.

[0047] like Figure 1 As shown, the first syringe 33 is slidably connected to the longitudinal groove 11 on the left side of the handle 10, and the second syringe 43 is slidably connected to the longitudinal groove 11 on the right side of the handle 10; the bottom of the handle 10 is provided with two parallel transverse grooves 12, and the top of the handle 10 is also provided with two parallel transverse grooves (not shown in the figure). The lower slide column of the first syringe 33 can slide left and right in the two transverse grooves 12 at the bottom, and the upper slide column of the second syringe 43 can slide left and right in the two transverse grooves at the top.

[0048] Combination Figure 4 As shown, when the first syringe 33 slides to the front limit block 13 and abuts against it, the downward slide column of the first syringe 33 just gets into the horizontal slide groove 12 at the bottom, so that the first syringe 33 can slide left and right in the handle 10. Specifically, by sliding the first syringe 33 to the right in the handle 10, the needle of the first injection needle 31 can be inserted into the right intestinal wall of the mouse. Similarly, when the second syringe 43 slides to the front limit block 13 and abuts against it, the upward slide column of the second syringe 43 just gets into the horizontal slide groove at the top, so that the second syringe 43 can slide left and right in the handle 10. Specifically, by sliding the second syringe 43 to the left in the handle 10, the needle of the second injection needle 41 can be inserted into the left intestinal wall of the mouse.

[0049] like Figure 4 As shown, in another preferred embodiment, a push block 38 is fixedly connected to the rear end of the first push rod 32. When the first injection needle 31 moves to the right and inserts into the right intestinal wall of the mouse, and the second injection needle 41 moves to the left and inserts into the left intestinal wall of the mouse, the push block 38 moves to the right and abuts against the rear end of the second push rod 42. Thus, by pushing the push block 38 forward, tumor cells and antibiotic solution can be injected simultaneously.

[0050] like Figures 1-3As shown, in another preferred embodiment, the first syringe 33 and the second syringe 43 are also connected to a latch 39. The left side of the first syringe 33 is elastically connected to a first slider 35 via a first elastic unit 36, and the first slider 35 is slidably connected to the longitudinal groove 11 on the left side of the handle 10. The right side of the second syringe 43 is elastically connected to a second slider 45 via a second elastic unit 46, and the second slider 45 is slidably connected to the longitudinal groove 11 on the right side of the handle 10. The first elastic unit 36 ​​and the second elastic unit 46 are both multiple columnar springs spaced apart along the length of the handle 10.

[0051] like Figures 1-3 As shown, under normal conditions, the first syringe 33 and the second syringe 43 are locked together by the latch 39 for self-locking. At this time, the first elastic unit 36 ​​and the second elastic unit 46 are compressed and stored energy, allowing the first syringe 33 and the second syringe 43 to slide in the longitudinal groove 11. Specifically, the first syringe 33 can slide back and forth in the handle 10 by sliding the first slider 35 back and forth in the longitudinal groove 11 on the left; the second syringe 43 can slide back and forth in the handle 10 by sliding the second slider 45 in the longitudinal groove 11 on the right.

[0052] When tumor cells and antibiotic solution need to be injected, the lock 39 is released. Under the elastic force of the first elastic unit 36, the sliding column of the first syringe 33 slides to the right in the transverse groove 12 at the bottom, so that the first injection needle 31 is inserted into the right intestinal wall of the mouse; under the elastic force of the second elastic unit 46, the sliding column of the second syringe 43 slides to the left in the transverse groove at the top, so that the second injection needle 41 is inserted into the left intestinal wall of the mouse.

[0053] like Figure 4 and Figure 5 As shown, in another preferred embodiment, the first syringe 33 contains tumor cells and petroleum jelly located behind the tumor cells. The tumor cells and petroleum jelly are separated by a first septum 37 slidably connected to the first syringe 33. The front end of the first syringe 33 has a spike 48 capable of piercing the first septum 37. The second syringe 43 contains antibiotic solution and petroleum jelly located behind the antibiotic solution. The antibiotic solution and petroleum jelly are separated by a second septum 47 slidably connected to the second syringe 43. The front end of the second syringe 43 also has a spike 48 capable of piercing the second septum 47.

[0054] The operator pushes the push block 38 forward, causing the first push rod 32 to push the first piston 34, and the second push rod 42 to push the second piston 44 forward. Tumor cells in the first syringe 33 are injected into the right intestinal wall of the mouse through the first injection needle 31, and antibiotic solution in the second syringe 43 is injected into the left intestinal wall of the mouse through the second injection needle 41. After the injection of tumor cells and antibiotic solution is completed, the operator moves the first syringe 33 to the left to withdraw the needle of the first injection needle 31 from the right intestinal wall of the mouse, and simultaneously moves the second syringe 43 to the right to withdraw the needle of the second injection needle 41 from the left intestinal wall of the mouse.

[0055] Next, push the pusher 38 forward to move the first piston 34 and the second piston 44 forward. The first diaphragm 37 and the second diaphragm 47 also move forward and are punctured by the corresponding spikes 48. The petroleum jelly in the first syringe 33 is discharged from the first injection needle 31 to seal the puncture point on the right intestinal wall of the mouse, preventing the antibiotic solution from leaking out of the intestinal wall. At the same time, the petroleum jelly in the second syringe 43 is discharged from the second injection needle 41 to seal the puncture point on the left intestinal wall of the mouse, preventing tumor cells from leaking out of the intestinal wall. After injecting petroleum jelly, return the first injection device 30 and the second injection device 40 to their initial state, then deflate the balloon 22 to a contracted state, and then remove the syringe from the mouse's anus by holding the handle 10.

[0056] like Figure 4 and Figure 5 As shown, in another preferred embodiment, a marker block 49 is fixed to the side wall of the first push rod 32 / second push rod 42. For example, the marker block 49 is located on the first push rod 32. When the first diaphragm 37 / second diaphragm 47 advances to the spike 48, the marker block 49 is aligned with the rear end of the first syringe 33 to remind people that the tumor cells and antibiotic solution have been injected.

[0057] like Figure 4 and Figure 5 As shown, more preferably, the inner wall of the handle 10 is movably connected to a locking block 14 that can intermittently abut against the outer walls of the first syringe 33 and the second syringe 43. For example, a locking block 14 is movably connected to the left and right walls at the rear end of the handle 10. Normally, the locking blocks 14 are folded up, not affecting the left and right movement of the first syringe 33 and the second syringe 43. After the first injection needle 31 and the second injection needle 41 exit the mouse intestinal wall, the right locking block 14 is unfolded. The right locking block 14 overcomes the elastic force of the first elastic unit 36 ​​and abuts against the right wall of the first syringe 33, fixing the positions of the first syringe 33 and the first injection needle 31, facilitating subsequent injection of Vaseline; the left locking block 14 is unfolded. The left locking block 14 overcomes the elastic force of the second elastic unit 46 and abuts against the left wall of the second syringe 43, fixing the positions of the second syringe 43 and the second injection needle 41, facilitating subsequent injection of Vaseline.

[0058] In the description of this specification, references to terms such as "preferred embodiment," "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0059] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A mouse intestinal tumor cell injector, characterized in that, It includes a hollow handle, an injection device installed in the handle, and a radially expandable support ring structure connected to the front end of the handle; The injection device includes a first injection needle, a second injection needle, a first push rod, a second push rod, a first injection cylinder, and a second injection cylinder. A first piston and a second piston are slidably connected to the first and second injection cylinders, respectively. The first injection needle and the second injection needle are respectively connected to the outlets at the front ends of the first and second injection cylinders. The first push rod and the second push rod are respectively fixed to the first piston and the second piston. The handle is sleeved over the first and second injection cylinders. The first and second injection cylinders are slidably connected to the handle through grooves on the handle. The first and second injection cylinders are both arranged parallel to the length direction of the handle. The first and second injection cylinders are located on the left and right sides inside the handle and are staggered vertically. The first injection needle bends to the right from the front end of the first injection cylinder, and the second injection needle bends to the left from the front end of the second injection cylinder. By operating the first plunger, tumor cells can be expelled from the first injection needle, and by operating the second plunger, antibiotics can be expelled from the second injection needle. When the support ring structure is in a contracted state, the first injection needle and the second injection needle are located inside the support ring structure. When the support ring structure is in the unfolded support state, the tips of both the first and second injection needles can extend to the outer edge of the support ring structure and insert into the mouse intestinal wall. The slide includes a longitudinal slide extending along the length of the handle and a transverse slide extending along the width of the handle. When the first and second injection cylinders are slidably connected to the longitudinal groove, the first and second injection needles can extend out to the outer edge of the support ring structure or retract into the support ring structure. When the first and second syringes are slidably connected in the transverse groove, the first and second injection needles can be inserted into the intestinal wall of the mouse.

2. The mouse intestinal tumor cell injector according to claim 1, characterized in that, The first and second syringes are also connected to a locking buckle. The first syringe is elastically connected to a first slider through a first elastic unit, and the second syringe is elastically connected to a second slider through a second elastic unit. The first slider and the second slider are slidably connected in corresponding longitudinal grooves. When the first and second injection cylinders are locked by the latch for self-locking, the first and second injection cylinders can slide in the longitudinal groove; After the lock is released, under the elastic force of the first elastic unit and the second elastic unit, the first syringe and the second syringe can slide in the corresponding transverse grooves and insert the first injection needle and the second injection needle into the mouse intestinal wall.

3. The mouse intestinal tumor cell injector according to claim 1, characterized in that, The front end of the longitudinal slide has a front limit stop.

4. The mouse intestinal tumor cell injector according to claim 1, characterized in that, A push block is fixedly connected to the rear end of the first push rod. When the first injection needle and the second injection needle are inserted into the intestinal wall of the mouse, the push block abuts against the rear end of the second push rod.

5. The mouse intestinal tumor cell injector according to any one of claims 1-4, characterized in that, The first syringe contains tumor cells and petroleum jelly located behind the tumor cells. The tumor cells and petroleum jelly are separated by a first septum that is slidably connected to the first syringe. The front end of the first syringe has a spike that can puncture the first septum. The second syringe contains an antibiotic solution and petroleum jelly located behind the antibiotic solution. The antibiotic solution and petroleum jelly are separated by a second diaphragm that is slidably connected to the second syringe. The front end of the second syringe also has a spike that can puncture the second diaphragm.

6. The mouse intestinal tumor cell injector according to claim 5, characterized in that... A marking block is fixed to the side wall of the first push rod / second push rod. When the first diaphragm / second diaphragm advances to the spike, the marking block is aligned with the rear end of the first syringe / second syringe. The inner wall of the handle is movably connected to a locking block that can intermittently abut against the outer walls of the first and second syringes.

7. The mouse intestinal tumor cell injector according to any one of claims 1-4, characterized in that, The support ring structure includes a ring body fixedly connected to the front end of the handle, and an airbag fixedly connected to the ring body and surrounding it. The airbag has an inflated support state and a deflated contracted state. The airbag is connected to an inflation / deflation device through a pipeline.

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