Dilution method for a sample analyzer and sample analyzer

Abstract

The application discloses a dilution method of a sample analyzer and the sample analyzer, the sample analyzer comprising a control module, a sampling module and a reagent module, the control module being connected with the sampling module and the reagent module respectively, the dilution method comprising the following steps: the control module controls the sample analyzer to enter a dilution mode based on the concentration of a sample; the control module acquires the sample amount collected by the sampling module and judges whether the sample amount is less than a preset value; if yes, the control module controls the reagent module to add a diluent and / or a first reagent into a first reaction cup and controls the sampling module to add the sample into the first reaction cup; if no, the control module controls the sampling module to add the sample into the first reaction cup and controls the reagent module to add the diluent and / or the first reagent into the first reaction cup. In the above manner, the requirement of high dilution ratio of a high-concentration sample to be tested can be met, and the reagent consumption and the reaction solution amount in the reaction cup required by the test process are reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the medical technology field, in particular to a dilution method of a sample analyzer and the sample analyzer. BACKGROUND

[0002] Generally, as people's various demands for medical technology increase, when using medical equipment for detection and analysis, users often hope to maintain the convenience of automatic medical equipment and the detection accuracy of medical equipment.

[0003] Generally, to realize sample detection of medical equipment, a medical equipment sample needle is often used to suck a certain amount of sample outside the machine, the sample is added to a clean reaction cup, a reagent needle is used to add a specific reaction reagent solution to the sample, and after the sample and the required reagent solution are fully reacted, the sample is detected.

[0004] Currently, because the concentration of some samples is too high, exceeding the linear detection range of the instrument, the general processing method is to add a sample diluent to the reaction cup, dilute the high-concentration sample in the reaction cup to a suitable reaction concentration according to a certain proportion, and then perform the subsequent test process. However, different immunological test items correspond to different samples with different concentrations, and when the concentration of the sample needs to be diluted too much, the same dilution process cannot reasonably arrange the dilution of high-concentration samples. SUMMARY

[0005] The first aspect of the embodiment of the present application provides a dilution method of a sample analyzer, the sample analyzer comprising a control module, a sampling module and a reagent module, the control module being connected with the sampling module and the reagent module, the dilution method comprising: the control module controls the sample analyzer to enter a dilution mode based on the concentration of the sample; the control module acquires the sample amount collected by the sampling module, and determines whether the sample amount is less than a preset amount value; if yes, the control module controls the reagent module to add a diluent and / or a first reagent to a first reaction cup, and controls the sampling module to add the sample to the first reaction cup; if no, the control module controls the sampling module to add the sample to the first reaction cup, and controls the reagent module to add the diluent and / or the first reagent to the first reaction cup.

[0006] The second aspect of the embodiment of the application provides a sample analyzer, which comprises a control module, a sampling module and a reagent module connected with the control module; the control module is used for controlling the sample analyzer to enter a dilution mode based on the concentration of a sample; the control module is also used for acquiring a sample amount collected by the sampling module, judging whether the sample amount is less than a preset value; if yes, the control module is further used for controlling the reagent module to add a diluent and / or a first reagent to a first reaction cup and controlling the sampling module to add the sample to the first reaction cup; if no, the control module is further used for controlling the sampling module to add the sample to the first reaction cup and controlling the reagent module to add the diluent and / or the first reagent to the first reaction cup.

[0007] The application has the beneficial effect that: the control module of the sample analyzer controls and schedules different dilution modes, the reagent module is controlled to add a diluent and / or a first reagent when the sample amount is less than a preset value, and the sampling module is controlled to add the sample; the sampling module is controlled to add the sample when the sample amount is greater than the preset value, and then the reagent module is controlled to add the diluent and / or the first reagent, so that the requirement of a high dilution ratio of a high-concentration sample to be tested is met, and because the sample amount that the reagent module absorbs is certain, the reagent consumption and the reaction solution amount in the reaction cup required by the test process are reduced. BRIEF DESCRIPTION OF DRAWINGS

[0008] In order to more clearly illustrate the technical solutions in the embodiments of the application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the application, and other drawings can be obtained by those skilled in the art without any creative effort on the basis of these drawings.

[0009] Figure 1 is a dilution method flowchart of a sample analyzer in an embodiment of the application;

[0010] Figure 2 is a flowchart of a specific embodiment of step S11 in the application; Figure 1

[0011] Figure 3 is a dilution flowchart of the sample analyzer entering a standard dilution mode in the application;

[0012] Figure 4 is a dilution flowchart of the sample analyzer entering a single-cup dilution mode in the application;

[0013] Figure 5 is a dilution flowchart of the sample analyzer entering a multiple-cup dilution mode in the application;

[0014] Figure 6 is a dilution flowchart of the sample analyzer entering a non-release mode in the application;​

[0015] Figure 7 is a liquid path diagram of a cleaning module of a sample analyzer of the present application;

[0016] Figure 8 is a schematic block diagram of a hardware architecture of a sample analyzer of the present application. DETAILED DESCRIPTION

[0017] In the following description, for purposes of explanation and not limitation, specific details are set forth such as particular architectures, techniques, etc. in order to provide a thorough understanding of the embodiments of the present application. However, it will be apparent to those skilled in the art that the present application can be practiced in other embodiments that depart from these specific details. In other instances, detailed descriptions of well-known methods, devices, circuits, and

[0018] It will be understood that the term "includes," "including," "has," "having," "comprises," "comprising" or "contains," "containing" when used in this specification and in the following claims, specifies the presence of stated features, integers, steps, operations, elements, or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or groups thereof.

[0019] It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting. As used in this specification and the appended claims, the singular forms "a," "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

[0020] It will be further understood that the terms "and / or," "comprises," "comprising," "has," "having," "includes" and / or "including" when used in this specification and in the following claims, are taken to be open-ended terms. That is, the term "comprises" and / or "comprising" is intended to mean one or more of the listed items "comprise" at least one of these items and "include" one or more of these items.

[0021] As used in this specification and the appended claims, the term "if" can be construed to mean "when" or "once" or "in response to determining" or "in response to detecting" depending on the context. Similarly, the phrase "if it is determined" or "if [a described condition or event] is detected" can be construed to mean "once it is determined" or "in response to determining" or "once [the described condition or event] is detected" or "in response to detecting [the described condition or event]," depending on the context.

[0022] In order to illustrate the technical solutions of the present application, the following specific embodiments are described. Please refer to Figure 1 , Figure 1is a dilution method flowchart of an embodiment of a sample analyzer of the present application. The sample analyzer comprises a control module, a sampling module and a reagent module. The control module is connected to the sampling module and the reagent module. The dilution method comprises the following steps:

[0023] S11: The control module controls the sample analyzer to enter a dilution mode based on the concentration of the sample.

[0024] The sample analyzer is provided with multiple dilution modes for different sample concentrations. When the sample concentration is in different concentration ranges, the control module calls the dilution mode corresponding to the sample concentration to dilute the sample.

[0025] Specifically, the control module controls the sampling module to collect the sample and can obtain the sample concentration. The control module controls the sample analyzer to enter the dilution mode based on the concentration of the sample.

[0026] S12: The control module obtains the sample amount collected by the sampling module and determines whether the sample amount is less than a preset value.

[0027] While the control module controls the sampling module to collect the sample, the control module also obtains the sample amount collected by the sampling module so as to know how much the obtained sample amount is.

[0028] The sample analyzer is provided with a preset value for the collected sample, which is used to determine the sample amount obtained by the control module.

[0029] If yes, indicating that the sample amount is less than the preset value, then step S13 is entered, that is, the control module controls the reagent module to add diluent and / or first reagent to the first reaction cup, and controls the sampling module to add the sample to the first reaction cup.

[0030] Specifically, whether to add diluent or first reagent to the first reaction cup needs to detect the sample concentration. Generally, a concentration range value is preset. If the sample concentration is greater than the concentration range value, it indicates that the sample concentration is too high, and the first reaction cup adds diluent. If the sample concentration is less than the concentration range value, it indicates that the sample concentration is very low and does not need to be diluted, and the first reaction cup can add the first reagent.

[0031] Of course, those skilled in the art can also determine in other ways to reasonably select and process the operation, which is not limited here.

[0032] If no, indicating that the sample amount is greater than or equal to the preset value, then step S14 is entered, that is, the control module controls the sampling module to add the sample to the first reaction cup, and controls the reagent module to add diluent and / or first reagent to the first reaction cup.

[0033] Therefore, the application controls different dilution modes by the control module of the sample analyzer. When the sample amount is less than the preset amount, the control module controls the reagent module to add diluent and / or first reagent, and then controls the sampling module to add the sample. When the sample amount is greater than the preset amount, the control module controls the sampling module to add the sample, and then controls the reagent module to add the diluent and / or the first reagent, so as to meet the requirement of high dilution ratio of high-concentration sample to be tested, and because the sample amount controlled by the reagent module is certain, the reagent consumption and the reaction solution amount in the reaction cup in the test process are reduced.

[0034] Further, based on the sample concentration, it is determined whether dilution is needed. If yes, different dilution modes are entered according to the sample concentration.

[0035] The dilution modes in the sample analyzer of the embodiment of the application include at least one of a standard dilution mode, a single-cup dilution mode and a multiple-cup dilution mode. Please refer to Figure 2 , Figure 2 is a flowchart of a specific embodiment of step S11 in the application Figure 1 . The control module controls the sample analyzer to enter the dilution mode based on the sample concentration, and specifically includes the following steps:

[0036] S21: The control module determines whether the sample concentration is within a first preset range.

[0037] If the control module determines that the sample concentration is within the first preset range, step S22 is entered, that is, the control module controls the sample analyzer to enter the standard dilution mode based on the sample concentration.

[0038] If it is out of the first preset range, step S23 is entered, that is, the control module determines whether the sample concentration is within a second preset range.

[0039] If the control module determines that the sample concentration is within the second preset range, step S24 is entered, that is, the control module controls the sample analyzer to enter the single-cup dilution mode based on the sample concentration.

[0040] If it is out of the second preset range, step S25 is entered, that is, the control module determines whether the sample concentration is within a third preset range.

[0041] If the control module determines that the sample concentration is within the third preset range, step S26 is entered, that is, the control module controls the sample analyzer to enter the multiple-cup dilution mode based on the sample concentration.

[0042] Therefore, for the sample concentration, by setting the first preset range value, the second preset range value and the third preset range value, different dilution modes are scheduled respectively, which is beneficial for different sample concentrations to adopt the most suitable dilution mode for sample dilution.

[0043] Further, when the sample analyzer enters the standard dilution mode, please refer to Figure 3 , Figure 3 is a dilution process schematic diagram of the sample analyzer entering the standard dilution mode of the present application; the control module controls the reagent module to add diluent and / or the first reagent to the first reaction cup, and the step of controlling the sampling module to add the sample to the first reaction cup includes:

[0044] S31: The control module controls the sample analyzer to enter the standard dilution mode based on the concentration of the sample;

[0045] S32: The control module acquires the sample amount collected by the sampling module, and judges whether the sample amount is less than a preset amount value; usually, the preset amount value can be 65uL.

[0046] If yes, step S33 is entered, that is, the control module controls the reagent module to add diluent to the first reaction cup, controls the sampling module to add the sample below the liquid level of the diluent to the first reaction cup, and controls the reagent module to add the first reagent to the first reaction cup; specifically, because the sample amount is too small, it is often impossible to drop when dropping on the liquid level in the reaction cup, and the sample is often easy to hang on the sampling needle, so the needle tip of the sampling needle needs to be immersed below the liquid level of the reaction cup, and when the sample amount is small, the accuracy of the sampling needle can be improved by immersing the needle tip below the liquid level for sampling.

[0047] If no, step S34 is entered, that is, the control module controls the sampling module to add the sample to the first reaction cup, and controls the reagent module to add the diluent and the first reagent to the first reaction cup; further, the control module can also control the reagent module to add the second reagent to the first reaction cup, and whether to add the second reagent to the first reaction cup can be operated according to actual needs, which is not limited here.

[0048] Further, the sample analyzer further comprises a cup adding module and a second reaction cup connected with the control module; when the sample analyzer enters the single split cup dilution mode, please refer to Figure 4 , Figure 4 is a dilution process schematic diagram of the sample analyzer entering the single split cup dilution mode of the present application; the control module is used for controlling the cup adding module to add the second reaction cup, the control module controls the reagent module to add diluent and / or the first reagent to the first reaction cup, and the step of controlling the sampling module to add the sample to the first reaction cup includes:

[0049] S41: The control module controls the sample analyzer to enter single-dilution mode based on the sample concentration;

[0050] S42: The control module obtains the sample quantity collected by the sampling module and determines whether the sample quantity is less than the preset value;

[0051] If so, proceed to step S43, that is, the control module controls the reagent module to add diluent to the first reaction cup, and controls the sampling module to add the sample to the first reaction cup below the surface of the diluent;

[0052] If not, proceed to step S44, whereby the control module controls the sampling module to add the sample to the first reaction cup, controls the reagent module to add diluent to the first reaction cup, controls the reagent module to add the first reagent to the second reaction cup, and controls the sampling module to aspirate the diluted sample from the first reaction cup and add it to the second reaction cup.

[0053] Furthermore, the sample analyzer also includes a cup-adding module connected to the control module and a second reaction cup;

[0054] When the sample analyzer enters the multiple dilution mode, please refer to [link / reference]. Figure 5 , Figure 5 This is a schematic diagram of the dilution process of the sample analyzer entering the multiple dilution mode in this application; the control module is used to control the addition module to add a second reaction cup, the control module controls the reagent module to add diluent and / or first reagent to the first reaction cup, and the control module controls the sampling module to add the sample to the first reaction cup. The steps include:

[0055] S51: The control module controls the sample analyzer to enter the multiple dilution mode based on the sample concentration;

[0056] S52: The control module obtains the sample quantity collected by the sampling module and determines whether the sample quantity is less than the preset value;

[0057] If so, proceed to step S53, that is, the control module controls the reagent module to add diluent to the first reaction cup and the second reaction cup respectively, controls the sampling module to add the sample to the first reaction cup below the surface of the diluent, and controls the sampling module to aspirate the diluted sample from the first reaction cup and add it to the second reaction cup.

[0058] If not, proceed to step S54, whereby the control module controls the sampling module to add the sample to the first reaction cup, controls the reagent module to add diluent to the first reaction cup, controls the reagent module to add diluent to the second reaction cup, controls the sampling module to aspirate the diluted sample from the first reaction cup and add it to the second reaction cup, and controls the reagent module to add the first reagent to the second reaction cup.

[0059] Furthermore, please refer to Figure 6 , Figure 6is a dilution process schematic diagram of the sample analyzer of the present application entering a non-release mode; the dilution mode of the dilution method includes a non-dilution mode, specifically including the following steps:

[0060] S61: The control module determines that the concentration of the sample is within the fourth preset range value, and then the control module controls the sample analyzer to enter a non-dilution mode based on the concentration of the sample;

[0061] S62: The control module acquires the sample amount collected by the sampling module, and determines whether the sample amount is less than a preset amount value;

[0062] If yes, proceed to step S63, i.e., the control module controls the reagent module to add a first reagent to the first reaction cup, and controls the sampling module to add the sample below the liquid level of the first reagent into the first reaction cup;

[0063] If no, proceed to step S64, i.e., the control module controls the sampling module to add the sample into the first reaction cup, and controls the reagent module to add the first reagent into the first reaction cup.

[0064] Further, the sample analyzer further includes a mixing module connected to the control module, specifically, in different dilution modes, the mixing module performs different mixing operations, which can specifically include the following three mixing process operations:

[0065] When the sample analyzer is in a standard dilution mode, the mixing module is used to mix the liquid in the first reaction cup after the control module controls the sampling module to add the sample into the first reaction cup, and the reagent module to add the diluent and the first reagent into the first reaction cup.

[0066] When the sample analyzer is in a single split cup dilution mode, the mixing module is used to mix the liquid in the first reaction cup after the control module controls the sampling module to add the sample into the first reaction cup, and the reagent module to add the diluent into the first reaction cup.

[0067] When the sample analyzer is in a multiple split cup dilution mode, the mixing module is used to mix the liquid in the first reaction cup after the control module controls the sampling module to add the sample into the first reaction cup, and the reagent module to add the diluent into the first reaction cup; the mixing module is also used to mix the liquid in the second reaction cup after the control module controls the sampling module to add the diluted sample, the diluent and the first reagent in the first reaction cup into the second reaction cup.

[0068] Further, the mixing module can specifically be a stirring module, which is used to stir the liquid in the first reaction cup or the second reaction cup in response to the control of the control module.

[0069] In addition, the stirrer can also stir when there are multiple liquids in the first reaction cup or the second reaction cup, such as sample + first reagent, sample + diluent, sample + diluent + multiple reaction reagents, where "multiple" means a positive integer greater than or equal to 2, and the multiple reaction reagents at least include the first reagent and the second reagent.

[0070] Further, the sample analyzer further comprises a cleaning module, please refer to Figure 7 , Figure 7 is a liquid path diagram of the cleaning module of the sample analyzer of the present application; the cleaning module is connected with the sampling module, the reagent module and the control module; wherein the control module is used to control the cleaning module to clean the sampling module and the reagent module.

[0071] Wherein, the sample analyzer further comprises a first controllable valve 711 and a second controllable valve 721, the first controllable valve 711 is arranged between the sampling module and the cleaning module, and the second controllable valve 721 is arranged between the reagent module and the cleaning module.

[0072] Generally, the cleaning module comprises a cleaning pump 701, an in-machine buffer storage tank 702 and an out-machine buffer solution storage box 703, the cleaning pump 701 can draw the buffer solution in the out-machine buffer solution storage box 703 to the in-machine buffer storage tank 702, wherein the buffer solution in the out-machine buffer solution storage box 703 can clean the inner and outer walls of the sampling needle, and can also clean the inner and outer walls of the reagent needle.

[0073] The first controllable valve 711 and the second controllable valve 721 respectively have a first port, a second port and a third port. The sampling module comprises a sampling needle 712 and a sampling syringe 713. The reagent module comprises a reagent needle 724, a first reagent syringe 722 and a second reagent syringe 723.

[0074] Specifically, the sampling needle cleaning process is as follows:

[0075] 1. The controller controls the first port 1 of the first controllable valve 711 to be connected with the first port 2 of the first controllable valve 711, and controls the sampling syringe 713 to suck the buffer solution from the in-machine buffer storage tank 702.

[0076] 2. The third port 3 of the first controllable valve 711 is connected with the second port 2 of the first controllable valve 711, and the controller controls the sampling syringe 713 to push the buffer solution into the sampling needle 712, so that the buffer solution cleans the inner wall of the sampling needle 712.

[0077] The reagent needle cleaning process is as follows:

[0078] 1. The controller controls the second controllable valve 721 to connect the third port 3 of the second controllable valve 721 to the second port 2 of the second controllable valve 721, and controls the first reagent injector 722 to draw the buffer from the in-machine buffer storage tank 702;

[0079] 2. The controller controls the first port 1 of the second controllable valve 721 to connect to the third port 3 of the second controllable valve 721, and controls the first reagent injector 722 to push the buffer to the second reagent injector 723, and then controls the reagent needle 724 to be pushed again, so that the buffer washes the inner wall of the reagent needle 724.

[0080] In addition, the present application also provides a sample analyzer, please refer to Figure 8 , Figure 8 is a schematic block diagram of the hardware architecture of the sample analyzer of the present application, the sample analyzer 8 comprises a control module 81, a sampling module 82 and a reagent module 83 connected to the control module 81;

[0081] The control module 81 is used to control the sample analyzer 8 to enter a dilution mode based on the concentration of the sample;

[0082] The control module 81 is also used to obtain the sample amount collected by the sampling module 82, and determine whether the sample amount is less than a preset amount value;

[0083] If yes, the control module 81 is also used to control the reagent module 83 to add a diluent and / or a first reagent to a first reaction cup, and control the sampling module 82 to add the sample to the first reaction cup;

[0084] If no, the control module 81 is also used to control the sampling module 82 to add the sample to the first reaction cup, and control the reagent module 83 to add the diluent and / or the first reagent to the first reaction cup.

[0085] Therefore, the control module 81 of the sample analyzer 8 of the present application controls and schedules different dilution modes, when the sample amount is less than the preset amount value, the reagent module 83 is controlled to add the diluent and / or the first reagent, and then the sampling module 82 is controlled to add the sample; when the sample amount is greater than the preset amount value, the sampling module 82 is controlled to add the sample, and then the reagent module 83 is controlled to add the diluent and / or the first reagent, so as to meet the requirement of high dilution ratio of high-concentration sample to be tested, and because the sample amount sucked by the reagent module 83 is certain, the reagent consumption and the reaction solution amount in the reaction cup required by the test process are reduced.

[0086] Further, the sample analyzer 8 further comprises an incubation module, a cup adding module 84 and a mixing module 85.

[0087] The cup adding module 84 comprises a reaction cup warehouse, a reaction cup upper cup assembly, and a reaction cup adding slide. The control module 81 controls the cup adding module 84 to add the reaction cup to the reaction cup upper cup assembly and the reaction cup adding slide.

[0088] The incubation module comprises a disc structure with a rotation center, also referred to as an incubation disc. The disc structure is provided with a first position for placing a first reaction cup and a second position for placing a second reaction cup. The control module 81 controls the cup adding module 84 to add the first reaction cup to the first position and the second reaction cup to the second position.

[0089] The sampling module 82 comprises a sampling motor, a sampling needle 712, and a sampling syringe. The sampling syringe is connected to the sampling motor, and the sampling needle 712 is connected to the sampling syringe through a pipeline. The control module 81 controls the sampling motor to enable the sampling syringe to suck or spit liquid through the pipeline, and the liquid at least comprises a sample.

[0090] The reagent module 83 comprises a reagent motor, a reagent needle 724, and a reagent syringe. The reagent syringe is connected to the reagent motor, and the reagent needle 724 is connected to the reagent syringe through a pipeline. The control module 81 controls the reagent motor to enable the reagent syringe to suck or spit liquid through the pipeline, and the liquid at least comprises a diluent, a first reagent, and other reagents.

[0091] The mixing module 85 can be a stirring module, which can comprise a stirring motor and a stirring rod. After the reagent module 83 adds the diluent, the first reagent, or the sample to the first reaction cup or the second reaction cup, the control module 81 controls the stirring module to enable the stirring motor to drive the stirring rod to stir the liquid in the first reaction cup or the second reaction cup.

[0092] Further, the sample analyzer 8 can be a full-automatic immune analyzer, and can also be a blood detection analyzer, which is selected according to requirements, and is not limited here.

[0093] Generally, the sample analyzer 8 can determine the concentration of the sample and the project category. The project category has a corresponding concentration index. For example, if the sample concentration given for a project is 3-30 ug / L, dilution is not required, and if the sample concentration is greater than this range, dilution is required.

[0094] Specifically, the full-automatic immune analyzer has a conventional test process, which can be divided into four modes according to the sample concentration: a non-dilution mode, a standard dilution mode, a single-cup dilution mode, and a multi-cup dilution mode. Through the operation of the modules scheduled by the control module 81, the sample analyzer 8 can perform full-automatic dilution mode operation. The dilution mode operation process of a specific embodiment is as follows:

[0095] (1) Control module 81 controls the full-automatic immune analyzer to enter the non-dilution mode:

[0096] The sampling needle 712 moves to the sample collection position;

[0097] The sampling syringe 713 sucks a quantitative sample through the sampling needle 712;

[0098] The cup adding assembly adds a reaction cup in the incubation disc;

[0099] When the control module 81 obtains that the sample amount of the reagent module 83 is less than or equal to 65uL: the incubation disc transfers the reaction cup to the reagent adding position, the reagent needle 724 sucks a quantitative first reagent and adds it to the reaction cup, the incubation disc transfers the reaction cup to the sample adding position, the sampling needle 712 moves to the sample adding position, the sampling needle 712 immerses the needle tip under the liquid surface of the reaction cup and discharges the sample;

[0100] When the control module 81 obtains that the sample amount of the reagent module 83 is greater than 65uL: the incubation disc transfers the reaction cup to the sample adding position, the sampling needle 712 discharges the sample into the reaction cup, the incubation disc transfers the reaction cup to the reagent adding position, and the reagent needle 724 sucks a quantitative first reagent and adds it to the reaction cup;

[0101] After the sample in the reaction cup completes the first reagent addition, the sample is fully stirred and uniformly mixed by the stirring rod;

[0102] After the addition of all the reaction reagents is completed, the sample to be measured is subjected to a qualitative or quantitative measurement process by the instrument after the sample and the reagent are fully reacted.

[0103] (2) Control module 81 controls the full-automatic immune analyzer to enter the standard dilution mode:

[0104] The sampling needle 712 moves to the sample collection position;

[0105] The sampling syringe 713 sucks a quantitative sample through the sampling needle 712;

[0106] The cup adding assembly adds a reaction cup in the incubation disc;

[0107] When the control module 81 obtains that the sample amount of the reagent module 83 is less than or equal to 65uL: the incubation disc transfers the reaction cup to the reagent adding position, the reagent needle 724 sucks a quantitative diluent and adds it to the reaction cup, the incubation disc transfers the reaction cup to the sample adding position, the sampling needle 712 moves to the sample adding position, the sampling needle 712 immerses the needle tip under the liquid surface of the reaction cup and discharges the sample, and then the incubation disc transfers the reaction cup to the reagent adding position, and the reagent needle 724 sucks a quantitative first reagent and adds it to the reaction cup;

[0108] When the control module 81 obtains the sample amount of the reagent module 83≥65uL: the incubation disc transfers the reaction cup to the sample adding position, the sampling needle 712 discharges the sample into the reaction cup, the incubation disc transfers the reaction cup to the reagent adding position, and the reagent needle 724 sequentially adds the quantitative diluent and the first reagent into the reaction cup;

[0109] After the sample in the reaction cup completes the addition of the first reagent, the sample is fully stirred by the stirring rod;

[0110] After the addition of all the reaction reagents is completed, the instrument performs a qualitative or quantitative measurement process on the sample to be measured after the sample and the reagent are fully reacted.

[0111] The sampling needle 712 divides the sample into two cups for dilution, and the scheduling method is suitable for the case that the full-automatic immune analyzer enters the standard dilution mode and one reaction cup cannot meet the sample dilution requirement. The specific scheduling method is as follows:

[0112] (3) The control module 81 controls the full-automatic immune analyzer to enter the single-time cup-dilution mode:

[0113] The sampling needle 712 moves to the sample collection position;

[0114] The sampling syringe 713 sucks the quantitative sample through the sampling needle 712;

[0115] The cup adding assembly sequentially puts the first reaction cup and the second reaction cup into the incubation disc;

[0116] When the control module 81 obtains the sample amount of the reagent module 83≤65uL: the reagent needle 724 sucks the diluent and adds it into the first reaction cup, the sampling needle 712 immerses the sample below the liquid level of the first reaction cup, and the first reaction cup is transferred to the stirring and mixing position for full stirring;

[0117] When the control module 81 obtains the sample amount of the reagent module 83≥65uL: the sampling needle 712 adds the sample into the first reaction cup, the reagent needle 724 sucks the diluent and adds it into the first reaction cup, and the first reaction cup is transferred to the stirring and mixing position for full stirring;

[0118] The reagent needle 724 sucks the first reagent and adds it into the second reaction cup;

[0119] The sampling needle 712 sucks the quantitative sample that has been diluted from the first reaction cup and adds it into the second reaction cup;

[0120] After the sample in the reaction cup completes the addition of the first reagent, the sample is fully stirred by the stirring rod;

[0121] After the addition of all the reaction reagents is completed, the instrument performs a qualitative or quantitative measurement process on the sample to be measured after the sample and the reagent are fully reacted.

[0122] (4) The control module 81 controls the full-automatic immune analyzer to enter the multiple cup dilution mode.

[0123] The sampling needle 712 moves to the sample collection position;

[0124] The sampling syringe 713 sucks the quantitative sample through the sampling needle 712;

[0125] The cup adding assembly sequentially puts the first reaction cup and the second reaction cup into the incubation tray;

[0126] When the control module 81 obtains that the sample amount of the reagent module 83 is less than or equal to 65uL: the reagent needle 724 sucks the diluent and adds it into the first reaction cup, the sampling needle 712 immerses the first reaction cup below the liquid level to add the sample, and the first reaction cup is transferred to the stirring and mixing position for sufficient stirring;

[0127] When the control module 81 obtains that the sample amount of the reagent module 83 is greater than 65uL: the sampling needle 712 adds the sample into the first reaction cup, the reagent needle 724 sucks the diluent and adds it into the first reaction cup, and the first reaction cup is transferred to the stirring and mixing position for sufficient stirring;

[0128] The reagent needle 724 sucks the diluent and adds it into the second reaction cup;

[0129] The sampling needle 712 sucks the quantitative dilution sample from the first reaction cup and adds it into the second reaction cup;

[0130] The reagent needle 724 sucks the first reagent and adds it into the second reaction cup;

[0131] After the sample in the reaction cup completes the addition of the first reagent, the sample is sufficiently stirred through the stirring rod;

[0132] After the addition of all the reagents is completed, the sample is subjected to the qualitative or quantitative measurement process through the instrument after the sample and the reagents are sufficiently reacted.

[0133] Therefore, the application provides a sample dilution method, which can schedule the sampling needle 712 to dilute the sample in cups, and has the following technical advantages:

[0134] 1) The sample dilution process does not need manual operation, the high-concentration sample can automatically complete the dilution process in the instrument, the error caused by manual operation is reduced, and the detection efficiency and detection result accuracy of the instrument are improved;

[0135] 2) The diluent consumption in the sample dilution process is reduced, and the reagent consumption in the sample and reagent reaction process is reduced;

[0136] 3) The dilution ratio of the sample dilution in cups is adjusted to ensure that the sampling needle 712 and the reagent needle 724 suck and spit the liquid in a suitable range.

[0137] The above merely provides part of embodiments of the present application, and does not limit the protection scope of the present application, and any equivalent device or equivalent flow transformation, or direct or indirect application in other related technical fields, which are made by using the content of the present application specification and drawings, are also included in the patent protection scope of the present application.

Claims

1. A dilution method for a sample analyzer, characterized by, The sample analyzer comprises a control module, a sampling module and a reagent module, the control module is connected with the sampling module and the reagent module respectively, the dilution method comprises: The control module controls the sample analyzer to enter a dilution mode based on the concentration of the sample; The control module acquires the sample amount collected by the sampling module and judges whether the sample amount is less than a preset value; If yes, the control module controls the reagent module to add a diluent and / or a first reagent to a first reaction cup and controls the sampling module to add the sample to the first reaction cup; If no, the control module controls the sampling module to add the sample to the first reaction cup and controls the reagent module to add a diluent and / or a first reagent to the first reaction cup; The dilution mode comprises at least one of a standard dilution mode, a single cup dilution mode and a multiple cup dilution mode; The control module controls the sample analyzer to enter a dilution mode based on the concentration of the sample, comprising: If the control module judges that the concentration of the sample is within a first preset range, the control module controls the sample analyzer to enter a standard dilution mode based on the concentration of the sample; If the control module judges that the concentration of the sample is within a second preset range, the control module controls the sample analyzer to enter a single cup dilution mode based on the concentration of the sample; If the control module judges that the concentration of the sample is within a third preset range, the control module controls the sample analyzer to enter a multiple cup dilution mode based on the concentration of the sample; When the sample analyzer enters the standard dilution mode, the step of the control module acquiring the sample amount collected by the sampling module and judging whether the sample amount is less than the preset value comprises: If yes, the control module controls the reagent module to add the diluent to the first reaction cup, controls the sampling module to add the sample below the liquid level of the diluent to the first reaction cup and controls the reagent module to add the first reagent to the first reaction cup; If no, the control module controls the sampling module to add the sample to the first reaction cup and controls the reagent module to add the diluent and the first reagent to the first reaction cup.

2. The dilution method according to claim 1, characterized in that, The sample analyzer further comprises a cup adding module and a second reaction cup connected with the control module; When the sample analyzer enters the single cup dilution mode, the control module is used for controlling the cup adding module to add the second reaction cup, and the step of the control module acquiring the sample amount collected by the sampling module and judging whether the sample amount is less than the preset value comprises: If yes, the control module controls the reagent module to add the diluent to the first reaction cup and controls the sampling module to add the sample below the liquid level of the diluent to the first reaction cup; If not, the control module controls the sampling module to add the sample into the first reaction cup, controls the reagent module to add diluent into the first reaction cup, controls the reagent module to add the first reagent into the second reaction cup, and controls the sampling module to suck the diluted sample in the first reaction cup and add it into the second reaction cup.

3. The dilution method of claim 1, wherein, The sample analyzer further comprises a cup adding module and a second reaction cup connected with the control module; When the sample analyzer enters the multiple-time split-cup dilution mode, the control module controls the cup adding module to add the second reaction cup, and the control module acquires the sample amount collected by the sampling module and judges whether the sample amount is less than the preset amount value, which comprises: If yes, the control module controls the reagent module to add the diluent into the first reaction cup and the second reaction cup respectively, controls the sampling module to add the sample under the liquid surface of the diluent into the first reaction cup, and controls the sampling module to suck the diluted sample in the first reaction cup and add it into the second reaction cup; If not, the control module controls the sampling module to add the sample into the first reaction cup, controls the reagent module to add the diluent into the first reaction cup, controls the reagent module to add the diluent into the second reaction cup, controls the reagent module to suck the diluted sample in the first reaction cup and add it into the second reaction cup, and controls the reagent module to add the first reagent into the second reaction cup.

4. The dilution method of claim 1, wherein, The sample analyzer further comprises a mixing module connected with the control module; When the sample analyzer is in the standard dilution mode, the mixing module is used to mix the liquid in the first reaction cup after the control module controls the sampling module to add the sample into the first reaction cup and the reagent module to add the diluent and the first reagent into the first reaction cup; When the sample analyzer is in the single-time split-cup dilution mode, the mixing module is used to mix the liquid in the first reaction cup after the control module controls the sampling module to add the sample into the first reaction cup and the reagent module to add the diluent into the first reaction cup; When the sample analyzer is in the multiple-time split-cup dilution mode, the mixing module is used to mix the liquid in the first reaction cup after the control module controls the sampling module to add the sample into the first reaction cup and the reagent module to add the diluent into the first reaction cup, and the mixing module is further used to stir the liquid in the second reaction cup after the control module controls the sampling module to add the diluted sample, the diluent and the first reagent in the first reaction cup into the second reaction cup.

5. The dilution method of claim 1, wherein, The dilution mode comprises a non-dilution mode; When the control module judges that the concentration of the sample is in a fourth preset range value, the control module controls the sample analyzer to enter the non-dilution mode based on the concentration of the sample.

6. The dilution method according to any one of claims 1 to 4, characterized in that, The sample analyzer further comprises a cleaning module connected with the sampling module, the reagent module and the control module; the dilution method further comprises: The control module controls the cleaning module to clean the sampling module and the reagent module.

7. The dilution method according to claim 6, characterized in that, The sample analyzer further comprises a first controllable valve arranged between the sampling module and the cleaning module, and a second controllable valve arranged between the reagent module and the cleaning module.

8. A sample analyzer characterized by, The sample analyzer comprises a control module, a sampling module and a reagent module connected with the control module, and is applied to the dilution method according to any one of claims 1-7; The control module is configured to control the sample analyzer to enter a dilution mode based on the concentration of the sample; The control module is further configured to acquire the sample amount collected by the sampling module, and determine whether the sample amount is less than a preset amount value; If yes, the control module is further configured to control the reagent module to add a diluent and / or a first reagent to a first reaction cup, and control the sampling module to add the sample to the first reaction cup; If no, the control module is further configured to control the sampling module to add the sample to the first reaction cup, and control the reagent module to add the diluent and / or the first reagent to the first reaction cup; The dilution mode comprises at least one of a standard dilution mode, a single-cup dilution mode and a multiple-cup dilution mode; In response to the concentration of the sample being within a first preset range value, the control module is configured to control the sample analyzer to enter the standard dilution mode based on the concentration of the sample; In response to the concentration of the sample being within a second preset range value, the control module is configured to control the sample analyzer to enter the single-cup dilution mode based on the concentration of the sample; In response to the concentration of the sample being within a third preset range value, the control module is configured to control the sample analyzer to enter the multiple-cup dilution mode based on the concentration of the sample; When the sample analyzer enters the standard dilution mode, the control module is further configured to acquire the sample amount collected by the sampling module, and in response to the sample amount being less than the preset amount value, control the reagent module to add the diluent to the first reaction cup, control the sampling module to add the sample below the liquid level of the diluent to the first reaction cup, and control the reagent module to add the first reagent to the first reaction cup; In response to the sample amount being greater than or equal to the preset amount value, control the sampling module to add the sample to the first reaction cup, and control the reagent module to add the diluent and the first reagent to the first reaction cup.

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