Tobacco extract and its application in cigarette flavor enhancement

Abstract

The present application provides a tobacco extract, which is prepared by combining 50% and 70% alcohol extracts with tobacco leaf fragments as raw materials. The tobacco extract of the present application retains the relevant style characteristics of Dali tobacco, which will be of great benefit in making up for the lack of raw materials and reducing costs.

Description

Field of the invention:

[0001] The present application belongs to the field of food, and specifically, provides tobacco extracts and their application in cigarette flavoring. Background technology:

[0002] In order to improve the taste and mouthfeel of cigarettes, the traditional method of the cigarette industry is to apply tobacco flavorings to tobacco by adding materials or flavoring. Among tobacco flavorings, natural plant-derived tobacco flavorings account for a large proportion. At present, the research and development of natural tobacco flavorings is generally based on two aspects of research. One is to enrich the specific flavor components of natural extracts through various extraction technologies, including the application of some new technologies, and to obtain satisfactory results. The other is to pre-treat the raw materials through some technologies such as biotechnology to remove the components that have adverse effects on the taste, so as to ensure that the extracts contain more components that are beneficial to the taste. As far as the natural plant raw materials themselves are concerned, if the extracts that have certain benefits for the aroma and taste of cigarettes or can give cigarettes a certain aroma style can be prepared through the classic traditional extraction process, in principle, there is no need to pursue excessive refining using new technologies, which is beneficial to the operability of the industrial extraction process and the reduction of production costs. Sometimes it is not worth the loss to significantly increase the cost of spices through overly complicated refining operations and only bring a slight improvement in the texture of the spices.

[0003] The preparation of certain functional modules by compounding flavors for use in cigarette flavoring is a common practice in the process of cigarette flavoring, but most of them are modules based on specific functions, and compounding research based on a single source of plant raw materials is rare. The process of tobacco leaf threshing and re-baking will produce a large amount of tobacco leaf fragments that cannot be used in the formulation, and can only be transferred to related production enterprises as reconstituted tobacco raw materials. If these tobacco leaf fragments (especially high-end tobacco leaf fragments) can be fully utilized, and the relevant style characteristics of tobacco leaves from specific origins can be retained by making different fractions, and then the compounding process can be used to obtain tobacco flavor modules with a complete tobacco style from a specific origin, it will be of great benefit to make up for the lack of relevant raw materials in the unit. Summary of the invention:

[0004] This study selected Dali tobacco leaf crushing as raw material, retained the relevant style characteristics of Dali tobacco by making different fractions, and then used the compounding process to obtain a tobacco flavor module with a complete Dali tobacco style to supplement the demand for Dali tobacco fragrance style in the development of related products. It is helpful to inherit and sublimate the connotation and expression of Guangxi-style Chinese cigarettes; it is helpful to improve the technical content of Guangxi China Tobacco's tobacco products, and develop the vitality and new vitality of Zhenlong brand products; and make due contributions to the green, ecological and environmentally friendly scientific research guiding ideology of Guangxi China Tobacco and its product development.

[0005] In one aspect, the present application provides a tobacco extract, wherein the tobacco extract is obtained by ethanol extraction from tobacco leaf fragments.

[0006] On the other hand, the present application provides a method for preparing a tobacco extract, comprising the step of extracting tobacco leaf fragments with ethanol.

[0007] Furthermore, the tobacco leaf fragments are Dali tobacco leaf fragments after threshing.

[0008] Furthermore, the alcohol extraction includes 50% alcohol extraction and 70% alcohol extraction.

[0009] Furthermore, the 50% alcohol extraction comprises adding 5-15 times, preferably 10 times, the weight of the tobacco leaf fragments in 50% ethanol, heating to boiling, and extracting for 1-3 hours, preferably 2 hours.

[0010] Furthermore, the 70% alcohol extraction comprises adding 5-15 times, preferably 10 times, the weight of the tobacco leaf fragments with 70% ethanol, heating to boiling, and extracting for 1-3 hours, preferably 2 hours.

[0011] Furthermore, the alcohol extraction step further includes a concentration step; preferably includes two concentration steps; preferably includes reduced pressure concentration and rotary evaporation steps.

[0012] Furthermore, the ratio of 50% alcohol extract to 70% alcohol extract in the extract is 1-3:1; preferably 2:1.

[0013] On the other hand, the present application provides the use of the above tobacco extract in cigarette flavoring.

[0014] Furthermore, in the application, 0.5‰ of the mass of the shredded tobacco is added to the cigarette paper. Description of the drawings:

[0015] Figure 1 This is the total ion current diagram of the volatile and semi-volatile components of Dali tobacco leaf essence;

[0016] Figure 2 Evaluation record sheet for selecting the flavorings of this application for high-priced Zhenlong cigarettes.

[0017] Figure 3 Flowchart of this application study. Specific implementation method:

[0018] The present invention is described in detail below in conjunction with specific embodiments, but the protection scope of the present invention is not limited to the following embodiments.

[0019] The basic research process of this application is as follows Figure 3 shown.

[0020] Example 1 Finalization and optimization of extraction process parameters for different fractions

[0021] The crushed Dali tobacco leaves were selected as raw materials, and the following four conventional preparation methods were used to prepare the spice samples for use:

[0022] Water extraction and concentration: Take 50g of the plant, grind it, add ten times the amount of water, heat and extract twice, combine the extracts, filter them, and concentrate them under reduced pressure to a thick paste, then add double the amount of propylene glycol for later use.

[0023] Extraction and concentration with different concentrations of ethanol: 50g of the plant was crushed, and 95%, 70%, and 50% ethanol were added ten times the amount, respectively, and heated and extracted twice. The combined extracts were filtered and concentrated under reduced pressure to a thick paste, and propylene glycol was added twice for later use. A total of 3 samples were obtained.

[0024] Select the reference cigarettes for the test, dissolve the Dali tobacco leaf powder extracts prepared by the four processes into water or a certain concentration of ethanol. Use the test paper to evenly apply the four extracts on the cigarette paper at 0.5‰ of the tobacco mass, and evaluate their sensory quality. The results are shown in Table 1, which are all compared with the control cigarettes:

[0025] Table 1 Sensory evaluation table compared with control smoke

[0026]

[0027] The results showed that several Dali tobacco leaf fragment extracts can improve the aroma quality to a certain extent, increase the aroma volume, reduce impurities and irritation. Improve the aftertaste. Each extraction part has its own characteristics. Among them, the water extraction and the lower concentration ethanol extraction part mainly show a significant increase in sweetness, while the higher concentration ethanol extraction part is more manifested as an increase in the richness of tobacco aroma. Among them, the 50% ethanol extraction part and the 70% ethanol extraction part have a better comprehensive effect. In addition to the advantages of all parts, the 50% ethanol extraction part significantly increases the sweetness, and the characteristic aroma of Dali tobacco is obvious; the 70% ethanol extraction part has the advantages of increasing the richness of tobacco aroma of the high concentration alcohol extraction part. , It can also increase the sweetness and can increase the smoke concentration.

[0028] Based on the above results and the requirement that compound spices must be completely co-soluble in the same solvent system (for example, the water extract part and the 95% ethanol extract part are difficult to co-soluble in the same solvent system), we finally selected the 50% ethanol extract part and the 70% ethanol extract part, which are highly representative of the advantages and style characteristics of Dali tobacco leaves, as raw materials for subsequent compounding research.

[0029] Example 2 Study on the composition ratio of the fragrance module

[0030] The crushed Dali tobacco leaves were selected as raw materials, and the following three conventional preparation methods were used to prepare the spice samples for use:

[0031] Take 50g of the plant and grind it. Add ten times the amount of 50% and 70% ethanol respectively and heat and extract twice. After filtering the combined extracts, concentrate under reduced pressure to a thick paste. Add one times the amount of propylene glycol for later use. A total of 3 samples are obtained.

[0032] Compounding and evaluation: experimental reference tobacco was selected, and the Dali tobacco leaf powder and flavor samples prepared by three processes were dissolved in water according to the proportions shown in Table 6. They were evenly spread on cigarette paper with test flavor paper at 0.5‰ of the tobacco mass to evaluate their sensory quality. According to the comprehensive evaluation results, the best effect was achieved in the ratio of 50% ethanol extraction part: 70% ethanol extraction part (2:1), which was manifested as: better aroma quality, improved aroma volume, increased richness of tobacco aroma, increased concentration, reduced impurities, reduced irritation, clean and long aftertaste, and a sweet aftertaste. The laboratory technology was finalized and used as the basis for the ratio of industrial trial production.

[0033] Table 2 Evaluation table of different extraction parts of Dali tobacco leaves

[0034]

[0035] Example 3 Industrial production and technical finalization of functional tobacco flavoring

[0036] We went to Zhenlong Industrial Co., Ltd. to transfer the finalized laboratory production process to the industrialized production process in the workshop. After repeated tests, we established the industrialized production technology process route and achieved technical finalization. It was initially named YYJH01. The specific preparation process list is as follows:

[0037] Raw materials: Dali tobacco leaf fragments after threshing; anhydrous ethanol (or 95% edible ethanol), propylene glycol

[0038] 1.A Sample preparation:

[0039] 1.1 Raw materials: tobacco leaf fragments X kg, 50% (mass ratio) ethanol 20*X Kg (depending on the volume of the extraction tank, X≤28)

[0040] Preparation method of 50% ethanol: add 10X kg of anhydrous ethanol (or 95% edible ethanol) to the same mass of water, mix well, divide into two parts, and set aside.

[0041] 1.2 Extraction: Take X kg of shredded tobacco leaves, add 10X kg of 50% ethanol, heat to boiling (about 85-90℃), start timing from the boiling moment, and maintain for 2 hours. Open the drain port at the bottom of the extraction tank to drain the drainable liquid, add 10X kg of 50% ethanol to the extraction tank, boil and extract for 2 hours, drain the drainable extract, combine the extracts from the two extractions, and pass through a 200-mesh sieve for use.

[0042] 1.3 Initial concentration: Suck the extract into the single-effect concentration tank and start to concentrate under reduced pressure. Keep the temperature at 40°C and keep the system stable. If the concentration is slow in the later stage, the temperature can be slightly increased. Concentrate and extract to about 1.2X kg. Discharge the primary concentrate and pass it through a 200-mesh sieve for later use.

[0043] 1.4 Reconcentration: Take the filtrate and use a large rotary evaporation system. Start the temperature from 60°C until the system is stable, then slowly increase the temperature, control the maximum temperature not to exceed 80°C, and make the vacuum degree as high as possible, and concentrate to about 0.6X kg (yield 60%) extract.

[0044] 2.B Sample preparation:

[0045] 2.1 Raw materials: Y kg of tobacco leaf fragments, 20*Y kg of 70% (mass ratio) ethanol. (Based on the volume of the extraction tank, Y≤28)

[0046] Preparation method of 70% ethanol: 14Y kg ​​of anhydrous ethanol (or 95% edible ethanol), add 6Y kg of water, mix well, divide into two parts, and set aside.

[0047] 2.2 Extraction: Take Y kg of shredded tobacco leaves and add them to the extraction tank, add 10Y kg of 70% ethanol, heat to boiling (about 85-90℃), start timing from the boiling moment, and maintain for 2 hours. Open the drain port at the bottom of the extraction tank to drain the drainable liquid, add 10Y kg of 70% ethanol to the extraction tank, boil and extract for 2 hours, drain the drainable extract, combine the extracts from the two extractions, and pass through a 200-mesh sieve for later use.

[0048] 2.3 Initial concentration: Suck the extract into the single-effect concentration tank and start to concentrate under reduced pressure. Keep the temperature stable at 40°C and keep the system stable. If the concentration is slow in the later stage, the temperature can be slightly increased. Concentrate and extract to about Y kg, discharge the primary concentrate, pass it through a 200-mesh sieve and set it aside.

[0049] 2.4 Reconcentration: Take the filtrate and use a large rotary evaporation system. Start the temperature from 60°C until the system is stable, then slowly increase the temperature. Control the maximum temperature not to exceed 80°C. Keep the vacuum as high as possible and concentrate to about 0.5Y kg (yield 50%) of extract.

[0050] 3. Spices compounding:

[0051] 3.1 Raw materials: Sample A; Sample B; Propylene glycol

[0052] 3.2 Preparation: Add 2 parts of sample A to 1 part of sample B, mix well, then add 3 parts of propylene glycol and mix well.

[0053] Example 4 Ingredient Study

[0054] YYJH01 was subjected to simultaneous distillation and extraction, and its volatile and semi-volatile components were detected by GC / MS. The mass spectrum of each chromatographic peak was searched against the NIST spectral library and the WILEY spectral library, and the one with the highest matching degree was selected as its chemical component. The relative content of each component was calculated by the peak area normalization method. The results are shown in Table 3 and the ion flow diagram is shown in Figure 1 .

[0055] Table 3. Volatile and semi-volatile components of Dali tobacco leaf essence

[0056]

[0057]

[0058]

[0059]

[0060] According to Table 3, a total of 75 volatile and semi-volatile substances were detected in the YYJH01 flavor. Among them, there are representative ketone aroma components in tobacco, such as giant stigmatrienone, 4-(3-hydroxybutyl)-3,5,5-trimethyl-2-cyclohexene-1-one, aldehyde aroma components such as myristic aldehyde, 3-hydroxy-7,8-dihydro-ionol and other alcohol aroma components; ester aroma components such as methyl palmitate, palmitic acid, linoleic acid and other organic acid aroma components. There are also various non-tobacco-specific aroma components such as limonene, p-methylacetophenone, and triethyl phosphate. These aroma components have obvious effects on increasing sweetness and richness of tobacco aroma, and are of great significance for supplementing the original aroma of tobacco, especially the more complete Dali tobacco leaf style tobacco aroma. These refined and enriched aroma components should be the main material basis for YYJH01 to increase tobacco aroma and improve taste.

[0061] Example 5 Evaluation and Verification

[0062] After evaluation by 7 provincial judges (see the results Figure 2 ), YYJH01 is not much different from the laboratory trial product. This flavor can increase the original flavor of Yunnan tobacco, and the smoke is soft, sweet and mellow, with a clean aftertaste. It is suitable for use as a flavoring module flavor in Zhenlong high-priced cigarettes. At present, this achievement has entered the formula list of high-priced Zhenlong market research sample flavors and fragrances.

Claims

1. A tobacco extract, characterized in that The tobacco extract is prepared by the following method: (1) Adding 50 w / w% ethanol in an amount of 10 times the weight of the tobacco leaf fragments to the Dali tobacco leaf fragments after threshing, heating to boiling, and extracting for 2 hours; extracting twice; and combining the extracts; The extract is concentrated by reduced pressure concentration and rotary evaporation to 0.6 times the mass of tobacco leaf fragments; (2) adding 70 w / w% ethanol in an amount of 10 times the weight of the tobacco leaf fragments to the Dali tobacco leaf fragments after threshing, heating to boiling, and extracting for 2 hours; extracting twice; and combining the extracts; The extract is concentrated by reduced pressure concentration and rotary evaporation to 0.5 times the mass of tobacco leaf fragments; (3) The extract obtained in step (1) and step (2) and propylene glycol are mixed in a mass ratio of 2:1:3 to obtain a tobacco extract.

2. Use of the tobacco extract according to claim 1 in cigarette flavoring.

3. The use according to claim 2, wherein in the application, the tobacco extract is added to the cigarette paper at 0.5‰ of the weight of the shredded tobacco.

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