SSR molecular marker primer combination and method for identifying morchella sexta strain ZJYDJ001

By developing an SSR molecular marker primer combination and PCR amplification and fluorescence capillary electrophoresis detection method for the *Morchella esculenta* strain ZJYDJ001, the problem of inaccurate identification of *Morchella esculenta* strains was solved, enabling rapid and accurate strain identification and intellectual property protection, and improving cultivation stability and yield.

CN115927728BActive Publication Date: 2026-06-09KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
Filing Date
2022-11-22
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

In the existing technology, the names of the morel mushroom strains are confusing and the quality is inconsistent, resulting in unstable fruiting, low yield, poor repeatability, and a lack of effective molecular markers for variety identification and intellectual property protection.

Method used

A primer set of SSR molecular markers for the morel strain ZJYDJ001 was developed, including four pairs of primers: MSSR029, MSSR036, MSSR041, and MSSR092. Combined with PCR amplification and fluorescent capillary electrophoresis detection, the specific identification of the morel strain was achieved.

Benefits of technology

This method enables rapid and accurate identification of the morel strain ZJYDJ001, avoiding contamination with other strains, protecting intellectual property rights, and improving cultivation stability and yield.

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Abstract

The application discloses a SSR molecular marker primer combination and method for identifying Morchella sexta ZJYDJ001 strain, and the SSR molecular marker primer combination comprises four pairs of SSR molecular marker primers, namely MSSR029, MSSR036, MSSR041 and MSSR092. The method for identifying the Morchella sexta ZJYDJ001 strain based on the SSR molecular marker primer combination is realized according to the following steps: firstly, extracting mycelium genomic DNA of the Morchella sexta strain to be detected; then, performing PCR amplification on the DNA by using the SSR molecular marker primer combination; performing fluorescent capillary electrophoresis detection on the amplified PCR product; and comparing the fluorescent peak diagram, so that the Morchella sexta ZJYDJ001 strain can be identified. The method provided by the application can be used for rapidly identifying the Morchella sexta strain at the mycelium stage, avoids mixing the fine strain selected through breeding with other Morchella sexta strains in the production and operation and market flow process, thereby effectively protecting intellectual property rights, and has important significance for identifying the authenticity of the Morchella sexta strain in the production process.
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