A kind of cultivation method of scoparia seedlings

Abstract

The present invention provides a method for cultivating scopolamine seedlings, comprising land preparation, transplanting seedlings according to a spacing of (20-60) cm×50 cm, and field management, wherein the field management comprises fertilization, and the fertilization comprises the steps of applying base fertilizer in the soil for planting and fertilization after planting. The scopolamine medicinal material cultivated by the method of the present invention has good quality and high yield, and has excellent promotion and application value.

Description

Technical Field

[0001] The invention belongs to the field of plant cultivation, and particularly relates to a method for cultivating scoparia seedlings. Background Art

[0002] The Tibetan language of hyoscine is "Tangchuan Nabao", which is the dried root of Anisodus tanguticus (Maxim.) Pascher, a plant of the genus Anisodus Link et Otto of the Solanaceae family. Hyoscine is mainly distributed in plateau areas such as Qinghai and Tibet. It is a newly discovered medicinal resource in modern times. It is recorded in the Four Medical Classics, Jingzhu Materia Medica, Tibetan Medicine Jingjing Materia Medica, Chinese Tibetan Medicine Volume 1, and Chinese Tibetan Materia Medica. Jingzhu Materia Medica records: "The Illustrated Guide says: 'The root of black hyoscine is very large; the stem has nine branches, the leaves are black and thick, and are shaped like goose wings; the flowers are purple-black with a toxic smell, and the leaves and flowers are produced in the buds on the ground, so it is called 'Tang Zaohe'; the fruit pods are thick, hard, and bag-shaped; the seeds are flat, small, kidney-shaped, and black. It has the effect of killing insects and treating sores and skin anthrax'". Modern pharmacological research has also confirmed that its main ingredients, scopolamine and anisodine, have analgesic, antispasmodic, and detumescent functions. They are mainly used to treat pain and toothache caused by ulcers, acute and chronic gastroenteritis, gastrointestinal neurosis, etc., and are also used clinically to treat chronic bronchitis. Therefore, scopolamine is a Tibetan medicine with very high medicinal value.

[0003] The market for scopolamine is expanding and the demand is increasing. The yield of wild scopolamine is small and difficult to pick, which cannot meet people's demand for scopolamine. In addition, excessive harvesting of wild scopolamine plants will lead to damage to biological resources and ecological environment. In order to meet market demand, protect the plateau ecological environment, achieve sustainable utilization of biological resources and sustainable development of Tibetan medicinal materials, it is of great significance to explore and develop the introduction and cultivation technology of scopolamine based on the unique biological and ecological characteristics of the plateau plant scopolamine.

[0004] However, as a plateau plant, Scots dasyphylla has long adapted to the special environmental conditions of the plateau and has unique physiological and ecological characteristics. There are many difficulties in the research on the introduction, seedling raising and cultivation methods of this unique plant. Therefore, the current technologies for Scots dasyphylla seedling breeding, introduction, seedling raising and cultivation are still not mature enough, have not been systematized and are rarely reported. Summary of the invention

[0005] The purpose of the invention is to provide a method for cultivating scoparia seedlings.

[0006] The present invention provides a method for cultivating scoparia seedlings, comprising land preparation, transplanting and planting, and field management, wherein:

[0007] The transplanting and planting is as follows: taking the scopolamine seedlings and planting them in the field or greenhouse according to the plant spacing of (20-60) cm×50 cm;

[0008] The field management includes fertilization, and the fertilization includes applying basal fertilizer in the soil for planting and fertilization after planting; the fertilization after planting is at least one of the following methods: applying a single inorganic fertilizer, applying a mixed inorganic fertilizer, or applying an organic fertilizer;

[0009] The single inorganic fertilizer is nitrogen fertilizer, phosphate fertilizer or potash fertilizer, the nitrogen fertilizer application amount is 5-25 kg / mu, the phosphate fertilizer application amount is 25-45 kg / mu, and the potash fertilizer application amount is 2.5-7.5 kg / mu;

[0010] The mixed inorganic fertilizer is a combination of at least two of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer, wherein the nitrogen fertilizer application amount is 10-20 kg / mu, the phosphorus fertilizer application amount is 50-70 kg / mu, and the potassium fertilizer application amount is 7.5-12.5 kg / mu;

[0011] The organic fertilizer is cow dung-based fertilizer and / or sheep dung-based fertilizer, and the cow dung-based fertilizer is applied in an amount of 4 to 8 kg / m 2 , the amount of sheep manure base fertilizer applied is 0.75~3kg / m 2 .

[0012] Furthermore, the above-mentioned planting row spacing is 30cm×50cm.

[0013] Furthermore, the above-mentioned basal fertilizer is an inorganic fertilizer, and the inorganic fertilizer is a phosphorus fertilizer and a nitrogen fertilizer.

[0014] Furthermore, the above-mentioned phosphate fertilizer is diammonium phosphate, and the above-mentioned nitrogen fertilizer is urea, and the dosage is 25 kg / mu of diammonium phosphate and 10 kg / mu of urea.

[0015] Furthermore, the single inorganic fertilizer is nitrogen fertilizer, and the application amount is 20 kg / mu;

[0016] Or, the single inorganic fertilizer is phosphate fertilizer, and the application amount is 40kg / mu;

[0017] Alternatively, the single inorganic fertilizer is potassium fertilizer, and the application amount is 5 kg / mu.

[0018] Furthermore, the above-mentioned mixed inorganic fertilizer is a combination of nitrogen fertilizer and potassium fertilizer, the nitrogen fertilizer application amount is 10-15 kg / mu, and the potassium fertilizer application amount is 10 kg / mu; or, the mixed inorganic fertilizer is a combination of phosphorus fertilizer and potassium fertilizer, the phosphorus fertilizer application amount is 50-60 kg / mu, and the potassium fertilizer application amount is 10 kg / mu.

[0019] Furthermore, the above-mentioned mixed inorganic fertilizer is a combination of nitrogen fertilizer and potassium fertilizer, the nitrogen fertilizer application amount is 15kg / mu, and the potassium fertilizer application amount is 10kg / mu; or, the mixed inorganic fertilizer is a combination of phosphorus fertilizer and potassium fertilizer, the phosphorus fertilizer application amount is 60kg / mu, and the potassium fertilizer application amount is 10kg / mu.

[0020] Furthermore, the above-mentioned mixed inorganic fertilizer is a combination of nitrogen fertilizer and potassium fertilizer, and the cultivation site is Huzhu, Qinghai; the mixed inorganic fertilizer is a combination of phosphorus fertilizer and potassium fertilizer, and the cultivation site is Huangzhong, Qinghai.

[0021] Furthermore, the organic fertilizer is cow dung-based fertilizer and / or sheep dung-based fertilizer, and the cow dung-based fertilizer is used in an amount of 6 kg / m 2 , the amount of sheep manure base fertilizer is 1.5kg / m 2 .

[0022] The cultivation of medicinal plants is closely related to environmental conditions, soil, etc. Environmental conditions are composed of many ecological factors, including climate, soil, fertilizer, etc. These factors promote or restrict each other, and act on the growth and development process of medicinal plants in a comprehensive manner, and determine the yield and quality of medicinal materials to a certain extent. The method of the present invention cultivates scopolamine seedlings, which can make scopolamine medicinal materials have excellent quality and high yield, and has very good promotion and application value.

[0023] Obviously, according to the above contents of the present invention, in accordance with common technical knowledge and customary means in the art, without departing from the above basic technical ideas of the present invention, other various forms of modification, replacement or change may be made.

[0024] The above contents of the present invention are further described in detail below through specific implementation methods in the form of embodiments. However, this should not be understood as the scope of the above subject matter of the present invention being limited to the following examples. All technologies realized based on the above contents of the present invention belong to the scope of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is the average value of biological indicators in the scopolamine cultivation density test.

[0026] Figure 2 It is the average value of biological indexes in the nitrogen fertilizer experiment of Scots scoparia.

[0027] Figure 3 It is the average value of biological indicators of scopolamine phosphate fertilizer experiment.

[0028] Figure 4 It is the average value of biological indicators of scopolamine potassium fertilizer experiment.

[0029] Figure 5 These are the growth indicators of Scots daphne under different mixed fertilization gradients.

[0030] Figure 6 The yield of Scots daphne per mu under different mixed fertilization gradients. DETAILED DESCRIPTION

[0031] The scopolamine seedlings used in the present invention are: scopolamine seed sources from Golog Tibetan Autonomous Prefecture, and seedling sources obtained by breeding seedlings in Nianbo Town, Ledu County, Haidong City, Qinghai Province in 2017.

[0032] Example 1: Cultivation of Anisodactyla by the method of the present invention

[0033] In the fields or greenhouses in Huangzhong, Qinghai, land preparation and basal fertilizer application: diammonium phosphate 25kg / mu, urea 10kg / mu;

[0034] Take the seedlings of Scoparia scoparia and plant them in rows with a spacing of 30 cm × 50 cm;

[0035] After planting, first apply a single inorganic fertilizer: 20 kg / mu of urea, and then apply mixed inorganic fertilizers. The amount of the mixed inorganic fertilizers is: 15 kg / mu of urea and 10 kg / mu of potassium sulfate.

[0036] Other field management measures are carried out by those skilled in the art according to common knowledge and means in the art (for example, according to agricultural cultivation manuals), and thereafter, topdressing is performed once every 4 years.

[0037] Example 2: Cultivation of Anisodactyla by the method of the present invention

[0038] The single inorganic fertilizer applied was 40 kg / mu of diammonium phosphate, and the rest was cultivated according to the method of Example 1.

[0039] Example 3: Cultivation of Anisodactyla by the method of the present invention

[0040] The single inorganic fertilizer applied was 5 kg / mu of potassium sulfate, and the rest was cultivated according to the method of Example 1.

[0041] Example 4: Cultivation of Anisodactyla by the method of the present invention

[0042] In the fields or greenhouses of Huzhu, Qinghai, land preparation and basal fertilizer application: diammonium phosphate 25kg / mu, urea 10kg / mu;

[0043] Take the seedlings of Scoparia scoparia and plant them in rows with a spacing of 30 cm × 50 cm;

[0044] After planting, first apply a single inorganic fertilizer: 20 kg / mu of urea, and then apply mixed inorganic fertilizers. The amount of the mixed inorganic fertilizers is: 50 kg / mu of diammonium phosphate and 10 kg / mu of potassium sulfate.

[0045] Other field management measures are carried out by those skilled in the art according to common knowledge and means in the art (for example, according to agricultural cultivation manuals), and thereafter, topdressing is performed once every 4 years.

[0046] Example 5: Cultivation of Anisodactyla by the method of the present invention

[0047] The single inorganic fertilizer applied was 40 kg / mu of diammonium phosphate, and the rest was cultivated according to the method of Example 4.

[0048] Example 6: Cultivation of Anisodactyla by the method of the present invention

[0049] The single inorganic fertilizer applied was 5 kg / mu of potassium sulfate, and the rest was cultivated according to the method of Example 4.

[0050] Example 7: Cultivation of Anisodactyla by the method of the present invention

[0051] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 6kg / m2 of decomposed cow dung base fertilizer was applied. 2 , and the rest is cultivated according to the scheme of Example 1.

[0052] Example 8: Cultivation of Anisodactyla by the method of the present invention

[0053] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 1.5 kg / m2 of decomposed sheep manure was also applied. 2 , and the rest is cultivated according to the scheme of Example 1.

[0054] Example 9: Cultivation of Anisodactyla by the method of the present invention

[0055] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 6kg / m2 of decomposed cow dung base fertilizer was applied. 2 , and the rest is cultivated according to the scheme of Example 2.

[0056] Example 10: Cultivation of Anisodactyla by the method of the present invention

[0057] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 1.5 kg / m2 of decomposed sheep manure was also applied. 2 , and the rest is cultivated according to the scheme of Example 2.

[0058] Example 11: Cultivation of Anisodactyla by the method of the present invention

[0059] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 6kg / m2 of decomposed cow dung base fertilizer was applied. 2 , and the rest is cultivated according to the scheme of Example 3.

[0060] Example 12: Cultivation of Anisodactyla by the method of the present invention

[0061] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 1.5 kg / m2 of decomposed sheep manure was also applied. 2 , and the rest is cultivated according to the scheme of Example 3.

[0062] Example 13: Cultivation of Anisodactyla by the method of the present invention

[0063] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 6kg / m2 of decomposed cow dung base fertilizer was applied. 2 , and the rest is cultivated according to the scheme of Example 4.

[0064] Example 14: Cultivation of Anisodactyla by the method of the present invention

[0065] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 1.5 kg / m2 of decomposed sheep manure was also applied. 2 , and the rest is cultivated according to the scheme of Example 4.

[0066] Example 15: Cultivation of Anisodactyla by the method of the present invention

[0067] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 6kg / m2 of decomposed cow dung base fertilizer was applied. 2 , and the rest is cultivated according to the scheme of Example 5.

[0068] Example 16: Cultivation of Anisodactyla by the method of the present invention

[0069] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 1.5 kg / m2 of decomposed sheep manure was also applied. 2 , and the rest is cultivated according to the scheme of Example 5.

[0070] Example 17: Cultivation of Anisodactyla by the method of the present invention

[0071] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 6kg / m2 of decomposed cow dung base fertilizer was applied. 2 , and the rest is cultivated according to the scheme of Example 6.

[0072] Example 18: Cultivation of Anisodactyla by the method of the present invention

[0073] During the growth of seedlings, in addition to the mixed inorganic fertilizer, 1.5 kg / m2 of decomposed sheep manure was also applied. 2 , and the rest is cultivated according to the scheme of Example 6.

[0074] Comparative Example 1

[0075] No topdressing was performed during the field management process. The rest of the cultivation was carried out in accordance with the scheme of Example 1.

[0076] The beneficial effects of the method of the present invention are demonstrated by experimental examples below.

[0077] Experimental Example 1: Screening of the cultivation method of the present invention

[0078] 1.1 Effects of planting density on yield and quality of Scots daphne

[0079] 1.1.1 Experimental methods

[0080] The same method as in Example 1 was used to design the planting density, i.e. the spacing between rows and plants, as follows:

[0081] M1: 20cm×50cm; M2: 30cm×50cm; M3: 40cm×50cm; M4: 50cm×50cm; M5: 60cm×50cm. The seedlings of Hyoscyamus scoparia were cultivated, and the root length, root dry weight, root diameter and root fresh weight of the medicinal material Hyoscyamus scoparia were measured in the first year of cultivation.

[0082] 1.1.2 Experimental Results

[0083] The results of root length, root diameter, root fresh weight and root dry weight of Scots dasyphylla under different planting densities are shown in Figure 1 ,As can be seen from the figure, in the first year of planting Scotia ,the density was M4 (50 cm × 50 cm), and the average values ​​of root length, root dry weight, root diameter and root fresh weight were the largest.

[0084] The fresh weight of scopolamine per unit planting density was converted into scopolamine yield per mu, and the results are shown in Table 1:

[0085] Table 1 Yield per mu of the scopolamine density test

[0086] Planting density Yield per mu (jin / mu) M1 757.7 M2 721.8 M3 377.5 M4 437.5 M5 239.3

[0087] It can be seen that when the density is M1 and M2, the per mu output value is the largest. As the density decreases, the per mu output decreases. The preferred cultivation density is M4.

[0088] 1.2 Effect of nitrogen fertilizer (urea) application on the yield and quality of Scots scopolamine

[0089] The same method as in Example 1 was used, except that after planting, the following single inorganic fertilizer was applied for screening, and no mixed fertilizer was applied.

[0090] 1.2.1 Experimental methods

[0091] Designed nitrogen fertilizer application rate:

[0092] N1: 10 jin / mu; N2: 20 jin / mu; N3: 30 jin / mu; N4: 40 jin / mu; N5: 50 jin / mu; The seedlings of Hyoscyamus scoparia were cultivated, and the root length, root dry weight, root diameter and root fresh weight of the medicinal material Hyoscyamus scoparia were measured in the first year of cultivation.

[0093] 1.2.2 Experimental results

[0094] from Figure 2 It can be seen that when the amount of N fertilizer is increased, the biomass first decreases and then increases. When the amount of fertilizer is 40 jin / mu (20kg / mu), it reaches the maximum value and then decreases.

[0095] The fresh weight of scopolamine per unit planting density was converted into scopolamine yield per mu, and the results are shown in Table 2:

[0096] Table 2 Yield per mu of Scots daphne with different N fertilizers

[0097] N fertilizer application amount Yield per mu (jin / mu) N1 723.7 N2 634.0 N3 1110.3 N4 1314.8 N5 829.9

[0098] It can be seen that the yield per mu of scopolamine first decreases and then increases with the increase of N fertilizer. When the N fertilizer is 40 catties / mu, it reaches the maximum value. Therefore, when applying a single N fertilizer, it is preferred to use an application amount of 40 catties / mu (20 kg / mu).

[0099] 1.3 Effect of phosphate fertilizer (diammonium phosphate) application on the yield and quality of scopolamine

[0100] The same method as in Example 1 was used, except that after planting, the following single inorganic fertilizer was applied for screening, and no mixed fertilizer was applied.

[0101] 1.3.1 Experimental methods

[0102] Designed phosphorus fertilizer application rate:

[0103] P1: 50 jin / mu; P2: 60 jin / mu; P3: 70 jin / mu; P4: 80 jin / mu; P5: 90 jin / mu; The seedlings of Hyacinthus angustifolia were cultivated, and the root length, root dry weight, root diameter and root fresh weight of the medicinal material Hyacinthus angustifolia were measured in the first year of cultivation.

[0104] 1.3.2 Experimental Results

[0105] from Figure 3 It can be seen that the root length increases with the increase of P fertilizer application, and when the P fertilizer application reaches (80 jin / mu, 40 kg / mu), the root length reaches the maximum value. The root diameter and root fresh weight do not change much with the P fertilizer application, and when the P fertilizer application reaches (80 jin / mu), the root diameter and root fresh weight reach the maximum value.

[0106] The fresh weight of scopolamine per unit planting density was converted into scopolamine yield per mu, as shown in Table 3.

[0107] Table 3 Effects of different phosphate fertilizer application rates on the yield of Scots scoparia

[0108] P fertilizer application amount Yield per mu (jin / mu) P1 644.5 P2 601.3 P3 627.5 P4 765.7 P5 596.7

[0109] It can be seen that the yield per mu of scopolamine first decreases and then increases with the increase of P fertilizer. When the P fertilizer is 80 catties / mu, the yield per mu reaches the maximum. Therefore, when applying a single P fertilizer, it is preferred to use an application amount of 80 catties / mu (40 kg / mu).

[0110] 1.4 Effect of potassium fertilizer (potassium sulfate) application on the yield and quality of scopolamine

[0111] The same method as in Example 1 was used, except that after planting, the following single inorganic fertilizer was applied for screening, and no mixed fertilizer was applied.

[0112] 1.4.1 Experimental methods

[0113] Designed potassium fertilizer application rate:

[0114] K1: 10 jin / mu; K2: 15 jin / mu; K3: 20 jin / mu; K4: 25 jin / mu; K5: 30 jin / mu; The seedlings of Hyoscyamus scoparia were cultivated, and the root length, root dry weight, root diameter and root fresh weight of the medicinal material Hyoscyamus scoparia were measured in the first year of cultivation.

[0115] 1.4.2 Experimental Results

[0116] from Figure 3 It can be seen that when the application rate of potassium fertilizer is 10 jin / mu (5kg / mu), the overall growth of scopolamine is the best.

[0117] The fresh weight of the unit planting density of scopolamine was converted into the yield per mu of scopolamine, as shown in Table 4:

[0118] Table 4 Effects of different potassium fertilizer usage on the yield of Scots scopolamine

[0119] K fertilizer application amount Yield per mu (jin / mu) K1 736.8 K2 694.7 K3 545.3 K4 637.7 K5 608.8

[0120] It can be seen that the yield per mu of scopolamine first decreases and then increases with the increase of K fertilizer. When the K fertilizer is 10 catties / mu, the yield per mu reaches the maximum. Therefore, when applying a single K fertilizer, it is preferred to use an application amount of 10 catties / mu (5 kg / mu).

[0121] 1.5 Effect of mixed inorganic fertilizer application on the growth of Scots daphne

[0122] The same method as in Example 1 was used, except that after planting, the following mixed inorganic fertilizers were applied for dosage screening, and no single inorganic fertilizer was applied.

[0123] The experimental plots were set up in Huzhu and Huangzhong, Qinghai, and three fertilization factors were set, namely N fertilizer, P fertilizer, and K fertilizer. Four levels were set for each influencing factor, namely N0: no N fertilizer, N1: 10kg / mu, N2: 15kg / mu, N3: 20kg / mu, K0: no K fertilizer, K1: 7.5kg / mu, K2: 10kg / mu, K3: 12.5kg / mu, P0: no P fertilizer, P1: 50kg / mu, P2: 60kg / mu, P3: 70kg / mu. A randomized block experimental design was used, with 3 replicates for each gradient, a total of 42 experimental plots, and each plot size was 10m*6.5m.

[0124] The fertilization scheme is shown in Table 5:

[0125] Table 5 Fertilization scheme

[0126] serial number deal with Nitrogen fertilizer (N) kg / mu Phosphate fertilizer (P) kg / mu Potassium fertilizer (K) kg / mu 1 (Comparative Example 1) <![CDATA[N0P0K0]]> 0 0 0 2 <![CDATA[N0P2K2]]> 0 50 10 3 <![CDATA[N1P2K2]]> 10 60 10 4 <![CDATA[N2P0K2]]> 15 0 10 5 <![CDATA[N2P1K2]]> 15 50 10 6 <![CDATA[N2P2K2]]> 15 60 10 7 <![CDATA[N2P3K2]]> 15 70 10 8 <![CDATA[N2P2K0]]> 15 60 0 9 <![CDATA[N2P2K1]]> 15 60 7.5 10 <![CDATA[N2P2K3]]> 15 60 12.5 11 <![CDATA[N3P2K2]]> 20 60 10 12 <![CDATA[N1P1K2]]> 10 50 10 13 <![CDATA[N1P2K1]]> 10 60 7.5 14 <![CDATA[N2P1K1]]> 15 50 7.5

[0127] Test indicators:

[0128] Five samples were collected from each plot. After washing, the root length, root diameter, root fresh weight and leaf fresh weight were measured. After drying in the shade, the root dry weight and leaf dry weight were measured.

[0129] result:

[0130] The data of root length, root diameter, root fresh weight, leaf fresh weight, root dry weight, and leaf dry weight of samples from different planting locations are shown in Table 6. Figure 5 .

[0131] In the Huzhu plot, root length, root diameter, root fresh weight, leaf fresh weight, root dry weight, and leaf dry weight were not significantly affected by different graded gradients. In the Huangzhong District plot, root length, root diameter, leaf fresh weight, and leaf dry weight were significantly affected by different fertilization gradients, while root fresh weight and root dry weight were not significantly affected by different graded gradients.

[0132] Table 6 Effects of different mixed fertilization on the growth indexes of Scots daphne

[0133]

[0134]

[0135] According to the growth index of Scots dasyphylla, the yield per mu of Scots dasyphylla is converted. The yield per mu of Scots dasyphylla is shown in Table 7. Figure 6 It can be seen that in the Huangzhong plot, the per-acre yield of the above-ground and underground parts of Anisodus was the highest when the fertilization gradient was N2P0K2. In the Huzhu plot, the per-acre yield of the above-ground and underground parts of Anisodus was the highest when the fertilization gradient was N0P2K2.

[0136] Table 7 Yield per mu of Scots scopolamine

[0137] Mixed fertilization mode Fresh root weight (kg) Fresh leaf weight (kg) Root dry weight (kg) Leaf dry weight (kg) Mutual Aid 1 315.49 320.83 82.04 48.69 Mutual Aid 2 357.32 393.05 128.64 92.90 Mutual Aid 3 271.14 226.78 95.71 62.36 Mutual Aid 4 269.02 251.24 73.37 51.14 Mutual Aid 5 266.80 271.56 88.14 69.08 Mutual Aid 6 306.82 315.71 97.83 57.81 Mutual Aid 7 309.68 319.21 131.02 73.85 Mutual Aid 8 264.58 269.02 93.38 62.25 Mutual Aid 9 314.44 323.97 92.90 52.41 Mutual Aid 10 307.85 328.37 107.75 59.00 Mutual Aid 11 349.06 346.84 106.72 84.49 Mutual Aid 12 344.62 371.30 97.83 68.92 Mutual Aid 13 307.30 288.24 109.58 54.79 Mutual Aid 14 309.68 312.06 95.29 50.03 Huangzhong 1 1295.98 956.81 493.25 249.46 Huangzhong 2 1519.76 1451.73 546.27 334.50 Huangzhong 3 1446.72 1869.93 499.58 526.60 Huangzhong 4 1865.60 2176.09 610.64 526.26 Huangzhong 5 1377.69 1593.80 470.24 394.53 Huangzhong 6 1462.40 1344.01 498.92 340.84 Huangzhong 7 1397.37 1473.07 490.91 387.53 Huangzhong 8 1435.05 1429.38 511.59 328.83 Huangzhong 9 1624.15 1574.12 583.96 413.21 Huangzhong 10 1658.83 1821.24 571.62 441.89 Huangzhong 11 1717.53 1777.89 589.96 468.23 Huangzhong 12 1517.43 1662.83 565.28 459.90 Huangzhong 13 1605.14 1711.86 607.30 424.55 Huangzhong 14 1719.86 1468.40 589.29 365.85

[0138] 1.6 Effect of increasing the application of biological fertilizer on the growth of Scots daphne

[0139] The same method as in Example 1 was used, except that the following organic fertilizers were applied for screening after planting, and no inorganic fertilizer was applied. The growth and biomass of the cultivated Anisodactyla were tested and evaluated, and the results are shown in Tables 8 to 9.

[0140] Table 8 Effect of adding cow dung as base fertilizer on the growth of Scots scopolamine

[0141]

[0142]

[0143] Table 9 Effect of adding sheep manure as base fertilizer on the growth of Scots scopolamine

[0144]

[0145] As shown in Table 8, the application of cow dung as a basal fertilizer had a significant effect on the growth and biomass accumulation of Anisodus scoparia (P < 0.05). With the increase of the application amount, the biomass of Anisodus scoparia showed an upward trend. 2 When , the root biomass and total biomass increased significantly, about twice that of the control treatment, and the benefit was the best.

[0146] As shown in Table 9, the application of sheep manure as basal fertilizer had a significant effect on the growth and biomass accumulation of Anisodus scoparia (P < 0.05). With the increase of application rate, the biomass of Anisodus scoparia showed an upward trend. 2 When , the root and total biomass increased significantly, with increases of 206% and 188% respectively compared with the control, and the benefit was the best.

[0147] In summary, the present invention provides a cultivation method for Anisodactyla scoparia, and the obtained Anisodactyla scoparia medicinal material has good quality and high yield, and has excellent promotion and application value.

Claims

1. A method for cultivating scoparia seedlings, characterized in that: Including land preparation, transplanting and field management, including: The transplanting and planting is as follows: taking the scopolamine seedlings and planting them in the field or greenhouse according to the plant spacing of 50 cm×50 cm; The field management includes fertilization, and the fertilization includes applying basal fertilizer in the soil for planting and fertilization after planting; the basal fertilizer is inorganic fertilizer, the inorganic fertilizer is phosphate fertilizer and nitrogen fertilizer, the phosphate fertilizer is diammonium phosphate, and the nitrogen fertilizer is urea, and the dosage is 25kg / mu of diammonium phosphate and 10kg / mu of urea; the method of fertilization after planting is any one of the following: applying a single inorganic fertilizer, applying a mixed inorganic fertilizer or applying an organic fertilizer; The single inorganic fertilizer is nitrogen fertilizer, phosphate fertilizer or potash fertilizer, the nitrogen fertilizer application amount is 5-25 kg / mu, the phosphate fertilizer application amount is 25-45 kg / mu, and the potash fertilizer application amount is 2.5-7.5 kg / mu; The mixed inorganic fertilizer is a combination of at least two of nitrogen fertilizer, phosphate fertilizer and potash fertilizer, wherein the nitrogen fertilizer is applied in an amount of 10-20 kg / mu, the phosphate fertilizer is applied in an amount of 50-70 kg / mu, and the potash fertilizer is applied in an amount of 7.5-12.5 kg / mu; The organic fertilizer is cow dung-based fertilizer and / or sheep dung-based fertilizer, and the cow dung-based fertilizer is applied in an amount of 4-8 kg / m 2 , the amount of sheep manure base fertilizer applied is 0.75~3kg / m 2 .

2. The cultivation method according to claim 1, characterized in that The single inorganic fertilizer is nitrogen fertilizer, and the application amount is 20kg / mu; Or, the single inorganic fertilizer is phosphate fertilizer, and the application amount is 40kg / mu; Alternatively, the single inorganic fertilizer is potassium fertilizer, and the application amount is 5 kg / mu.

3. The cultivation method according to claim 1, characterized in that The mixed inorganic fertilizer is a combination of nitrogen fertilizer and potassium fertilizer, the nitrogen fertilizer application amount is 10-15 kg / mu, and the potassium fertilizer application amount is 10 kg / mu; or, the mixed inorganic fertilizer is a combination of phosphate fertilizer and potassium fertilizer, the phosphate fertilizer application amount is 50-60 kg / mu, and the potassium fertilizer application amount is 10 kg / mu.

4. The cultivation method according to claim 3, characterized in that The mixed inorganic fertilizer is a combination of nitrogen fertilizer and potassium fertilizer, the nitrogen fertilizer application amount is 15kg / mu, and the potassium fertilizer application amount is 10kg / mu; or, the mixed inorganic fertilizer is a combination of phosphorus fertilizer and potassium fertilizer, the phosphorus fertilizer application amount is 60kg / mu, and the potassium fertilizer application amount is 10kg / mu.

5. The cultivation method according to claim 3 or 4, characterized in that The mixed inorganic fertilizer is a combination of nitrogen fertilizer and potassium fertilizer, and the cultivation site is Huzhu, Qinghai; the mixed inorganic fertilizer is a combination of phosphorus fertilizer and potassium fertilizer, and the cultivation site is Huangzhong, Qinghai.

6. The cultivation method according to claim 1, characterized in that The organic fertilizer is cow dung-based fertilizer, and the application amount is 6kg / m 2 , or the organic fertilizer is sheep manure-based fertilizer, and the application amount is 1.5kg / m 2 .

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