A reagent aspiration control method, apparatus, system and storage medium

Abstract

This invention provides a reagent aspiration control method, device, system, and storage medium, relating to the field of medical testing device technology. The reagent aspiration control method includes: receiving a reagent addition command; determining whether the preceding reagent has been aspirated completely; if not, returning to determine whether the preceding reagent has been aspirated completely; if complete, determining that the threshold variable meets the reagent addition conditions, and determining whether the target reagent needs to be added; if addition is needed, executing the target reagent addition process; if not, setting the threshold variable to target reagent aspiration complete. This invention determines whether to execute the target reagent addition process by sequentially judging the reagent addition conditions and whether reagent addition is needed, thereby enabling orderly simultaneous or separate reagent addition or transfer operations on reaction tubes during in vitro diagnostic experiments, avoiding the timing chaos and interference caused by simultaneous operation of multiple sampling arms.

Description

Technical Field

[0001] This invention relates to the field of medical testing device technology, and more specifically, to a reagent aspiration control method, apparatus, system, and storage medium. Background Technology

[0002] In vitro diagnostics (IVD) refers to products and services that obtain clinical diagnostic information by testing human samples (blood, body fluids, tissues, etc.) outside the human body, thereby determining diseases or bodily functions. IVD products mainly consist of diagnostic equipment and diagnostic reagents.

[0003] In the in vitro diagnostics industry, different diagnostic methods require different testing equipment. To save space, existing testing equipment often integrates different diagnostic methods into the same instrument. Furthermore, when aspirating in vitro diagnostic reagents, multiple liquids need to be drawn from the reagent tray, and reagents need to be added and transferred to reaction tubes and reagent tubes separately or simultaneously, which can easily lead to timing issues. Additionally, the space available for in vitro diagnostic equipment is usually limited, and multiple sampling arms operating simultaneously can easily cause interference. Summary of the Invention

[0004] In view of the above, and to address the aforementioned technical problems, the present invention provides a reagent aspiration control method, comprising:

[0005] Receive a reagent addition instruction, the reagent addition instruction including the target reagent to be added and the reagent addition order;

[0006] Based on the order of reagent addition, determine whether the preceding reagents of the target reagent have been completely aspirated;

[0007] If the preceding reagents of the target reagent have not been completely aspirated, return to determine whether the preceding reagents of the target reagent have been completely aspirated;

[0008] If the preceding reagents for the target reagent have been aspirated, then the admission variable is determined to meet the reagent addition conditions.

[0009] If the admission variable is determined to meet the reagent addition conditions, then it is determined whether the target reagent needs to be added.

[0010] If the target reagent needs to be added, then the target reagent addition procedure shall be executed;

[0011] If the target reagent does not need to be added, then the admission variable is set to "target reagent aspiration complete".

[0012] Preferably, the reagent label corresponding to the target reagent kit is obtained, and the theoretical liquid level height of each well in the target reagent kit is determined based on the reagent label;

[0013] Obtain the actual liquid level height at the target well in the target kit;

[0014] Determine whether the height difference between the actual liquid level and the theoretical liquid level is within a preset threshold range; if it is within the preset threshold range, then the actual liquid level is taken as the liquid level of the target well in the target kit.

[0015] If the liquid level is not within the preset threshold range, the probe height is adjusted to detect the actual liquid level in the target well of the target kit.

[0016] Preferably, the step of using the actual liquid level as the liquid level of the target well in the target kit if the actual liquid level is within the preset threshold range includes:

[0017] If the height difference between the actual liquid level and the theoretical liquid level is within a preset threshold range for a set number of consecutive times, then the actual liquid level will be used as the liquid level of the target well in the target kit.

[0018] Preferably, the step of adjusting the probe detection height to detect the actual liquid level height of the target well in the target kit if the liquid level is not within the preset threshold range includes:

[0019] If the height difference between the actual liquid level and the theoretical liquid level is not within the preset threshold range for any of the consecutive set number of times, the probe detection height will be adjusted to the preset height value, and the actual liquid level height of the target well in the target kit will be detected again.

[0020] Preferably, a liquid level height determination counter is provided; the liquid level height determination counter is used to count the number of times the step of determining whether the height difference between the actual liquid level height and the theoretical liquid level height is within a preset threshold range is executed.

[0021] The reagent aspiration control method further includes:

[0022] The current count of the liquid level determination counter is obtained. If the current count reaches the execution count threshold, the target reagent kit is determined to be an invalid reagent kit, and the label discarding process for the target reagent kit is executed.

[0023] Preferably, the actual liquid level height of each well in the target kit is obtained, the actual number of portions used in each well is calculated, and the minimum value of the actual number of portions used in each well in the target kit is taken as the first actual number of portions used in the target kit.

[0024] Determine whether the first actual number of doses used is greater than the preset standard number of doses; if it is greater than the preset standard number of doses, then the preset standard number of doses is taken as the actual number of doses used for the target kit.

[0025] If the number of actual uses is not greater than the preset standard number of uses, then the first actual number of uses of the target kit shall be taken as the actual number of uses.

[0026] Preferably, the reagent aspiration control method further includes: after each well in the kit performs a reagent aspiration action, the actual number of reagents used for the corresponding well is decremented by 1; when the actual number of reagents used for a well is 0, the well is marked as an invalid well; after all wells on the kit are marked as invalid wells, the kit is marked as an invalid kit.

[0027] Furthermore, to address the aforementioned problems, the present invention also provides a reagent aspiration control device, comprising:

[0028] The receiving module is used to receive reagent addition instructions and determine whether the admission variables meet the reagent addition conditions.

[0029] The judgment module is used to determine whether the target reagent needs to be added when the reagent addition conditions are met.

[0030] An execution module is used to execute the target reagent addition process when the target reagent needs to be added;

[0031] The setting module is used to set the admission variable to "target reagent aspiration complete" when the target reagent does not need to be added.

[0032] In addition, to solve the above problems, the present invention also provides a reagent aspiration control system, including a memory and a processor, wherein the memory stores a reagent aspiration control program, and the processor runs the reagent aspiration control program to enable the reagent aspiration control system to perform the reagent aspiration control method as described above.

[0033] In addition, to solve the above problems, the present invention also provides a computer-readable storage medium storing a reagent aspiration control program, which, when executed by a processor, implements the reagent aspiration control method as described above.

[0034] This invention provides a reagent aspiration control method, apparatus, system, and storage medium. The method includes: receiving a reagent addition instruction; determining whether an access variable meets the reagent addition conditions; if the reagent addition conditions are met, determining whether a target reagent needs to be added; if the target reagent needs to be added, executing the target reagent addition process; if the target reagent does not need to be added, setting the access variable to "target reagent aspiration completed".

[0035] In the reagent aspiration control method of this invention, the reagent aspiration process is first determined based on the reagent aspiration command to see if the reagent aspiration conditions are met. If the conditions are met, the determination is made as to whether the target reagent needs to be added. The aspiration process can be executed separately or the threshold variable can be set to complete the aspiration of the target reagent based on the determination result. By sequentially determining the reagent aspiration conditions and whether the reagent needs to be added, this invention determines whether to execute the target reagent aspiration process. This enables the orderly and simultaneous or separate addition or transfer of reagents to the reaction tubes during in vitro diagnostic experiments, avoiding the timing disorder and interference caused by the simultaneous operation of multiple sampling arms. Attached Figure Description

[0036] Figure 1 This is a schematic diagram of the hardware operating environment involved in an embodiment of the reagent aspiration control method of the present invention;

[0037] Figure 2 This is a schematic flowchart of the first embodiment of the reagent aspiration control method of the present invention;

[0038] Figure 3 This is a first schematic diagram showing the positional relationship and execution sequence of reagent holes in the reagent tray in one embodiment of the reagent aspiration control method of the present invention.

[0039] Figure 4 This is a second schematic diagram showing the positional relationship and execution sequence of reagent holes in the reagent tray in one embodiment of the reagent aspiration control method of the present invention;

[0040] Figure 5 This is a flowchart illustrating steps S700 to S900a / S900b in the second embodiment of the reagent aspiration control method of the present invention.

[0041] Figure 6 This is a flowchart illustrating steps S1000 to S1100 in the second embodiment of the reagent aspiration control method of the present invention.

[0042] Figure 7 This is a flowchart illustrating steps S10 to S30 / S40 in the third embodiment of the reagent aspiration control method of the present invention.

[0043] Figure 8 This is a schematic diagram of the module connections of the reagent aspiration control device of the present invention.

[0044] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0045] The embodiments of the present invention are described in detail below, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout.

[0046] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0047] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0048] It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0049] like Figure 1 The diagram shown is a structural schematic of the hardware operating environment of the terminal involved in an embodiment of the present invention.

[0050] The reagent aspiration control system of this invention can be a PC, or a mobile terminal device such as a smartphone, tablet, or portable computer. The reagent aspiration control system may include: a processor 1001 (e.g., a CPU), a network interface 1004, a user interface 1003, a memory 1005, and a communication bus 1002. The communication bus 1002 is used to enable communication between these components. The user interface 1003 may include a display screen, an input unit such as a keyboard, or a remote control; optionally, the user interface 1003 may also include a standard wired interface or a wireless interface. The network interface 1004 may optionally include a standard wired interface or a wireless interface (e.g., a Wi-Fi interface). The memory 1005 may be a high-speed RAM memory or a stable memory, such as a disk storage device. Optionally, the memory 1005 may also be a storage device independent of the aforementioned processor 1001. Optionally, the reagent aspiration control system may also include RF (Radio Frequency) circuitry, audio circuitry, a Wi-Fi module, etc. Furthermore, the reagent aspiration control system may be configured with other sensors such as a gyroscope, barometer, hygrometer, thermometer, and infrared sensor, which will not be described in detail here.

[0051] Those skilled in the art will understand that Figure 1The reagent aspiration control system shown is not intended to limit it and may include more or fewer components than illustrated, or combine certain components, or have different component arrangements. Figure 1 As shown, the memory 1005, which is a computer-readable storage medium, may include an operating system, a data interface control program, a network connection program, and a reagent aspiration control program.

[0052] In summary, this invention determines whether to execute the target reagent addition process by sequentially judging the reagent addition conditions and whether reagent addition is required. This enables the orderly simultaneous or separate addition or transfer of reagents to the reaction tubes during in vitro diagnostic experiments using the detection equipment, avoiding the timing chaos and interference caused by the simultaneous operation of multiple sampling arms.

[0053] Example 1:

[0054] Reference Figure 2 The first embodiment of the present invention provides a reagent aspiration control method, comprising:

[0055] Step S100: Receive a reagent addition instruction, which includes the target reagent to be added and the order in which the reagents are added.

[0056] Step S200: Based on the reagent addition order, determine whether the preceding reagents of the target reagent have been completely aspirated.

[0057] The above-described embodiment pertains to a detection device capable of performing in vitro diagnostic tests; for example, it may include, but is not limited to, a multiplex luminescence detection device.

[0058] The reagent aspiration control method provided in this embodiment can be used to detect various samples, including but not limited to blood and body fluids; and can be used with various detection methods, such as chemiluminescence immunoassay and chemiluminescence immunoassay-multiplexed detection.

[0059] The aforementioned multiluminescent device can be an integrated unit comprising a sample tray, a washing tray, a reagent tray, and an incubation shaking tray; wherein the incubation shaking tray and the reagent tray rotate independently; the reagent tray may contain a reagent kit; the reagent kit may include multiple reagent wells, for example, it may include three reagent wells, namely reagent wells 1, 2, and 3.

[0060] refer to Figure 3 (First schematic diagram) Figure 4(Second schematic diagram) As shown, when the sample needs to be processed, the sample needs to be transferred from the sample tray to the incubation shaking tray. When multiple reagents need to be added to the reaction tube of the sample tray or washing tray, the sampling arm rotates to position A to draw reagents from reagent hole 1 or reagent hole 3, and rotates to position B to draw reagents from reagent hole 2. In order to avoid the sampling arm colliding and interfering at positions A and B, a control method is set for the process of simultaneously or separately drawing two or more reagents.

[0061] In this embodiment, the addition of reagents requires the execution of a corresponding addition process based on the reagent addition instruction. The reagent addition instruction can be received in real time or at scheduled intervals.

[0062] The aforementioned admission variables are variables that characterize whether the current execution action has been completed. For example, they may include: reagent A has been completely drawn up, reagent B has been completely drawn up, and reagent C has been completely drawn up. Based on the information in the admission variables, a judgment is made as to whether the corresponding reagent addition conditions are met, thereby further determining whether to add the target reagent.

[0063] The reagent addition order described above can be a sequence that has a certain order and includes the reagent names. According to this reagent addition order, the reagent addition actions can be performed in an orderly manner when executing the addition process.

[0064] By observing the order in which reagents are added, it is possible to determine whether the absorption process of the preceding reagents in the current sequence has been completed.

[0065] If the preceding reagents of the target reagent have not been completely aspirated, return to step S200 to determine whether the preceding reagents of the target reagent have been completely aspirated.

[0066] Step S300: If the preceding reagents for the target reagent have been aspirated, then the admission variable is determined to meet the reagent addition conditions.

[0067] Step S400: If the admission variable meets the reagent addition conditions, then determine whether the target reagent needs to be added.

[0068] Step S500: If the target reagent needs to be added, then the target reagent addition process is executed;

[0069] In step S600, if the target reagent does not need to be added, the admission variable is set to "target reagent aspiration complete".

[0070] As described above, once it is determined that the preceding reagent aspiration is complete, i.e., the reagent addition conditions are met, the target reagent aspiration action can be performed, transferring the target reagent into the reaction tube. If the preceding reagent aspiration is not complete, i.e., the aspiration process is not yet finished and needs to continue, then it is necessary to return to step S110 and re-determine whether the preceding reagent aspiration is complete, until the washing result is obtained.

[0071] In this embodiment, the reagent addition conditions are determined by whether the preceding reagents of the target reagent have been completely aspirated. Then, the target reagent is aspirated into the reaction tube. Otherwise, the judgment of the aspiration process of the preceding reagents is back-executed, which avoids the conflict between the aspiration process of the current target reagent and the preceding reagents, and realizes the orderly aspiration of the target reagent.

[0072] The following is a specific embodiment of the present invention. It should be noted that although the embodiment of the present invention uses three reagents and two robotic arms to absorb the reagents as an example, other reagents can also be absorbed using the method described in the present invention, which will not be repeated here.

[0073] Step 101: After the instrument is started, it receives the information that reagent needs to be added for the first time, and sets the initial access variable a to "Reagent 3 has been aspirated".

[0074] Step 102: Determine whether reagent 1 needs to be aspirated. If reagent 1 needs to be aspirated, proceed to step 103; otherwise, proceed to step 106.

[0075] Step 103: Determine whether the entry variable a indicates that reagent 3 has been completely aspirated. If so, proceed to step 104; otherwise, repeat step 103.

[0076] Step 104: Pipette reagent 1 into the reaction tube;

[0077] Step 105: Determine whether reagent 1 has been completely aspirated. If it has been completely aspirated, proceed to step 106; otherwise, repeat the process of checking step 105.

[0078] Step 106: Set the admission variable 'a' to 'Reagent 1 has been completely aspirated';

[0079] Step 107: Determine whether reagent 2 needs to be aspirated. If reagent 2 needs to be aspirated, proceed to step 108; otherwise, proceed to step 111.

[0080] Step 108: Determine whether the entry variable a indicates that reagent 1 has been completely aspirated. If so, proceed to step 109; otherwise, repeat step 108.

[0081] Step 109: Pipette reagent 2 into the reaction tube;

[0082] Step 110: Determine whether reagent 2 has been completely aspirated. If it has been completely aspirated, proceed to step 111; otherwise, repeat step 110.

[0083] Step 111: Set the admission variable 'a' to indicate that reagent 2 has been completely aspirated;

[0084] Step 112: Determine whether reagent 3 needs to be aspirated. If reagent 3 needs to be aspirated, proceed to step 113; otherwise, proceed to step 116.

[0085] Step 113: Determine whether the entry variable a indicates that reagent 2 has been completely aspirated. If so, proceed to step 114; otherwise, repeat step 113.

[0086] Step 114: Pipette reagent 3 into the reaction tube;

[0087] Step 115: Determine whether reagent 3 has been completely aspirated. If it has been completely aspirated, proceed to step 116; otherwise, repeat step 115.

[0088] Step 116: Set the admission variable 'a' to 'Reagent 3 has been completely aspirated';

[0089] Step 117: Determine if the process has ended. If yes, end the process; otherwise, proceed to step 102.

[0090] As mentioned above, after determining that the admission variables meet the reagent addition conditions, it is necessary to further determine whether the target reagent needs to be added. This includes two cases: if the target reagent needs to be added, the addition process is executed; if it does not need to be added, it is determined that the target reagent has been added, and the admission variable is then assigned the value that the target reagent has been absorbed.

[0091] This embodiment determines whether to execute the target reagent addition process by sequentially judging the reagent addition conditions and whether reagent addition is required. This enables the orderly simultaneous or separate addition or transfer of reagents to the reaction tubes during in vitro diagnostic experiments, avoiding the timing chaos and interference caused by the simultaneous operation of multiple sampling arms.

[0092] Example 2:

[0093] Reference Figure 5 The second embodiment of the present invention provides a reagent aspiration control method. Based on the above embodiment 1, after the instrument is started or after the instrument has stopped working and restarted, the reagent aspiration control method further includes:

[0094] Step S700: Obtain the reagent label corresponding to the target reagent kit, and determine the theoretical liquid level height of each well in the target reagent kit based on the reagent label;

[0095] It should be noted that the theoretical liquid level height of each well in the target kit is determined based on the usage of the kit. If the target kit is being used for the first time, the liquid level height of each well recorded on the target kit label is the theoretical liquid level height of each well in the target kit. If the target kit has been partially used, after the instrument is restarted, the actual liquid level height of each well measured last time by the target kit will be used as the theoretical liquid level height of the target kit.

[0096] Step S800: Obtain the actual liquid level height of the target well in the target kit;

[0097] In this embodiment, in order to improve the detection equipment for in vitro diagnostic testing when performing actions such as adding, aspirating, cleaning, discarding, and replacing reagents, a redundancy and fault-tolerant method is added for adding reagents.

[0098] The target reagent kit described above is labeled with a reagent kit label, which is used to determine the theoretical liquid level height for each well.

[0099] The theoretical liquid level mentioned above refers to the theoretical height to which reagent can be added to the well corresponding to the reagent label. This theoretical liquid level can be calculated by dividing the liquid capacity indicated on the well by the bottom area of ​​that well, using the following formula:

[0100]

[0101] For example, if well 1 of the target kit is labeled as storing 6000 μL of reagent A, then according to the formula, the theoretical liquid level height of well 1 is 6000 μL / the bottom area of ​​well 1.

[0102] The above-mentioned actual liquid level height refers to the actual height reached by the liquid at the current orifice position.

[0103] The two steps, S500 and S600, concerning the theoretical liquid level height and the actual liquid level height, can be executed either first or simultaneously; the execution order is not limited here.

[0104] Step S900: Determine whether the height difference between the actual liquid level and the theoretical liquid level is within a preset threshold range;

[0105] Step S900a: If the actual liquid level is within the preset threshold range, then the actual liquid level height is taken as the liquid level height of the target well in the target kit.

[0106] The aforementioned preset threshold range is an evaluation index for whether the liquid level in a well is within the standard range. If the height difference is still within the preset threshold range, it can be determined that the liquid volume in that well is consistent with the liquid volume indicated on the kit label.

[0107] Further, in step S900a, if the actual liquid level is within the preset threshold range, the liquid level is used as the liquid level of the target well in the target kit, including:

[0108] Step S910a: If the height difference between the actual liquid level and the theoretical liquid level for a set number of consecutive times is within a preset threshold range, then the actual liquid level is taken as the liquid level of the target well in the target kit.

[0109] In order to further improve the accuracy of detection and avoid detection errors, this embodiment can set multiple height differences between the actual liquid level and the theoretical liquid level.

[0110] The above-mentioned number of consecutive times refers to the number of times the liquid level detection is performed consecutively, such as 2 times, or 3-4 times, etc.

[0111] For example, if the number of consecutive counts is set to 2, then the following judgment is made:

[0112] Get the current number of judgments;

[0113] If the current number of judgments is 2, then proceed to step S900a;

[0114] If the current number of judgments is not 2, then wait for the preset delay time before returning to the execution step S700, or directly return to the execution step S700.

[0115] In step S900b, if the liquid level is not within the preset threshold range, the probe detection height is adjusted to detect the actual liquid level height of the target well in the target kit.

[0116] If the amount of reagent in the well is not detected, the detection height of the detection probe needs to be adjusted, and the actual liquid level of the target well needs to be detected again after the height is adjusted.

[0117] Further, in step S900b, if the liquid level is not within the preset threshold range, the probe detection height is adjusted to detect the actual liquid level height of the target well in the target kit, including:

[0118] In step S910b, if the height difference between the actual liquid level and the theoretical liquid level is not within the preset threshold range for any of the consecutive set number of times, the probe detection height is adjusted to the preset height value, and the actual liquid level of the target well in the target kit is detected again.

[0119] If any test fails to reach the preset threshold range after a set number of consecutive tests, it can be determined that the amount of reagent in the well cannot be detected. The test needs to be adjusted to the preset height value and repeated.

[0120] In this embodiment, the method is used to determine the actual liquid level height of each well in the target reagent kit, as well as each reagent kit on the reagent tray. During the determination, by setting a continuous set number of times, the actual and preset liquid level heights of the wells are repeatedly checked, improving the accuracy of the detection results. This avoids prematurely discarding reagents from each well before they are fully used, thus preventing reagent waste and improving reagent utilization efficiency.

[0121] Furthermore, a liquid level height determination counter is provided; the liquid level height determination counter is used to count the number of times the step of determining whether the height difference between the actual liquid level height and the theoretical liquid level height is within a preset threshold range is executed.

[0122] As mentioned above, the number of consecutive steps can be counted using a counter. Each time a judgment is made, the counter increments by 1.

[0123] Further reference Figure 6 The reagent aspiration control method further includes:

[0124] Step S1000: Obtain the current count of the liquid level height determination counter;

[0125] Step S1100: If the current count reaches the execution count threshold, the target reagent kit is determined to be an invalid reagent kit, and the label discarding process for the target reagent kit is executed.

[0126] When performing the test, the reagents in the kit may be in liquid or solid form. If they are solid and contain unprepared dry powder in the wells, it may be necessary to manually or automatically add the corresponding solution to dilute and prepare the reagents.

[0127] If the operator forgets (or for other reasons) and fails to add the required dry powder to the empty slot of the reagent kit for dilution, and instead directly places the dry powder into the device for testing, the liquid level of the target well (containing the dry powder) will not be detected during the test. If the detection is performed according to step S700, it will fall into an error loop of repeatedly performing the test, affecting the efficiency of the device operation and testing, or it may be judged as having run out of reagents and directly enter the discard process, thus wasting the reagents.

[0128] To address the aforementioned issues, in this embodiment, if the current count reaches the execution count threshold after multiple tests, it can be determined that the reagents in the kit have not been diluted and prepared. This kit is considered an invalid kit, and the test for the reagent quantity of the kit will no longer be performed repeatedly. Instead, the kit will be discarded with a label, allowing the operator to reconfigure the kit, add the appropriate solution, restore the label, and retest.

[0129] Example 3:

[0130] Reference Figure 7 The third embodiment of the present invention provides a reagent aspiration control method, which, based on the above embodiment 1, further includes:

[0131] Step S10: Obtain the actual liquid level height of each well in the target kit, calculate the actual number of uses for each well, and take the minimum value of the actual number of uses for each well in the target kit as the first actual number of uses for the target kit.

[0132] The actual number of reagents used in a well can be calculated from the actual liquid level in that well. For example, if reagent A is placed in well 1 of the target kit, and the actual liquid level is A1, the calculation can be performed using the following formula:

[0133] Actual liquid capacity Q1 = Actual liquid level height × Bottom area of ​​orifice 1;

[0134] Let q1 be the volume of reagent A drawn each time. Then, the following formula can be used to calculate:

[0135]

[0136] In the above formula, the actual number of portions used is a positive integer.

[0137] Step S20: Determine whether the first actual number of portions used is greater than the preset standard number of portions;

[0138] Step S30: If the number of kits exceeds the preset standard number, then the preset standard number will be used as the actual number of kits to be used in the target kit.

[0139] Step S40: If the number of actual uses is not greater than the preset standard number of uses, then the first actual number of uses of the target kit shall be taken as the actual number of uses.

[0140] Comparing the first actual number of uses with the preset standard number of uses, and evaluating each well of the current target kit using the preset standard number of uses, the following two scenarios emerge:

[0141] (1) If the first actual number of uses is greater than the preset standard number of uses, it can be determined that the actual liquid volume in the well is more than the liquid volume indicated on the label. Then the preset standard number of uses is taken as the actual number of uses for each well of the current kit.

[0142] (2) If the first actual number of used portions is less than or equal to the preset standard number of portions, it can be determined that the actual liquid volume in the current hole position is equal to or less than the liquid volume indicated by the label. Then the first actual number of used portions is taken as the current actual number of used portions.

[0143] Furthermore, the reagent aspiration control method also includes:

[0144] Step S50: After performing a reagent aspiration operation once for each well in the kit, decrement the actual number of reagents used for the corresponding well by 1.

[0145] Step S60: When the actual number of times a hole is used is 0, the hole is marked as an invalid hole.

[0146] In step S70, after all wells on the kit are marked as invalid wells, the kit is marked as an invalid kit.

[0147] It should be noted that after each well in the kit is aspirated once, the actual number of reagents used in that well is reduced by 1. When the actual number of reagents used in a well is 0, in order to protect the detection equipment and avoid invalid monitoring samples, the well is marked as invalid, regardless of whether there is any reagent remaining. Once all wells on the kit are marked as invalid, the kit is marked as invalid and can be replaced.

[0148] In addition, refer to Figure 8 This embodiment also provides a reagent aspiration control device, including:

[0149] The receiving module 10 is used to receive a reagent adding instruction, which includes the target reagent to be added and the order in which the reagents are added.

[0150] The judgment module 20 is used to determine, according to the order of reagent addition, whether the preceding reagents of the target reagent have been completely aspirated;

[0151] The judgment module 20 is also used to return to the judgment whether the preceding reagent of the target reagent has been completely aspirated when the preceding reagent of the target reagent has not been completely aspirated.

[0152] The judgment module 20 is also used to determine that the admission variable meets the reagent addition conditions when the preceding reagent of the target reagent is aspirated.

[0153] The judgment module 20 is also used to determine whether the target reagent needs to be added if the admission variable meets the reagent addition conditions.

[0154] The execution module 30 is used to execute the target reagent addition process when the target reagent needs to be added.

[0155] The setting module 40 is used to set the admission variable to "target reagent absorption complete" when the target reagent does not need to be added.

[0156] In addition, this embodiment also provides a reagent aspiration control system, including a memory and a processor. The memory stores a reagent aspiration control program, and the processor runs the reagent aspiration control program to enable the reagent aspiration control system to perform the reagent aspiration control method as described above.

[0157] In addition, this embodiment also provides a computer-readable storage medium storing a reagent aspiration control program, which, when executed by a processor, implements the reagent aspiration control method as described above.

[0158] The sequence numbers of the above embodiments of the present invention are for descriptive purposes only and do not represent the superiority or inferiority of the embodiments.

[0159] Through the above description of the embodiments, those skilled in the art can clearly understand that the methods of the above embodiments can be implemented by means of software plus necessary general-purpose hardware platforms. Of course, they can also be implemented by hardware, but in many cases the former is a better implementation method. Based on this understanding, the technical solution of the present invention, or the part that contributes to the prior art, can be embodied in the form of a software product. This computer software product is stored in a storage medium (such as ROM / RAM, magnetic disk, optical disk) as described above, and includes several instructions to cause a terminal device (which may be a mobile phone, computer, server, or network device, etc.) to execute the methods described in the various embodiments of the present invention. The above are only preferred embodiments of the present invention and do not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A reagent suction control method characterized by comprising: The control method comprises: receiving reagent adding instructions, the reagent adding instructions comprising target reagents that need to be added, and reagent adding sequences; determining whether the preceding reagent of the target reagent is completed according to the reagent adding sequences; if the preceding reagent of the target reagent is not completed, returning to determine whether the preceding reagent of the target reagent is completed; if the preceding reagent of the target reagent is completed, determining whether an admission variable meets a reagent adding condition; if the admission variable meets the reagent adding condition, determining whether the target reagent needs to be added; if the target reagent needs to be added, executing an adding process of the target reagent; if the target reagent does not need to be added, setting the admission variable as the target reagent being completed; The reagent suction control method further comprises: obtaining a reagent box label corresponding to a target reagent box, and determining a theoretical liquid level of each hole position in the target reagent box according to the reagent box label; obtaining an actual liquid level of a target hole position in the target reagent box; determining whether a height difference between the actual liquid level and the theoretical liquid level is within a preset threshold range; if the height difference is within the preset threshold range, taking the actual liquid level as the liquid level of the target hole position in the target reagent box; if the height difference is not within the preset threshold range, adjusting a probe detection height to detect the actual liquid level of the target hole position in the target reagent box; The reagent suction control method further comprises: obtaining actual liquid levels of each hole position of a target reagent box, calculating actual usage amounts of each hole position, and taking a minimum value of the actual usage amounts of each hole position in the target reagent box as a first actual usage amount of the target reagent box.

2. The reagent suction control method according to claim 1, wherein The if the height difference is within the preset threshold range, taking the actual liquid level as the liquid level of the target hole position in the target reagent box comprises: if the height difference between the actual liquid level and the theoretical liquid level is within the preset threshold range for a continuous preset number of times, taking the actual liquid level as the liquid level of the target hole position in the target reagent box.

3. The reagent suction control method according to claim 1, wherein The if the height difference is not within the preset threshold range, adjusting a probe detection height to detect the actual liquid level of the target hole position in the target reagent box comprises: if the height difference between the actual liquid level and the theoretical liquid level is not within the preset threshold range at any time for a continuous preset number of times, adjusting the probe detection height by a preset height value, and detecting the actual liquid level of the target hole position in the target reagent box again.

4. The reagent suction control method according to claim 2, wherein The method further comprises: a liquid level judgment counter is provided; and the liquid level judgment counter is used for counting the number of times of execution of the step of determining whether the height difference between the actual liquid level and the theoretical liquid level is within the preset threshold range. The reagent suction control method further comprises: obtaining a current count of the liquid level judgment counter, and if the current count reaches an execution number threshold, determining that the target reagent box is an invalid reagent box, and executing a label discarding process for the target reagent box.

5. The reagent suction control method according to claim 1, wherein The reagent suction control method further comprises: determining whether the first actual usage amount is greater than a preset standard amount; If the number of the target reagent kits is greater than the preset standard number, the preset standard number is taken as the actual use number of the target reagent kits; If the number of the target reagent kits is not greater than the preset standard number, the first actual use number of the target reagent kits is taken as the actual use number.

6. The reagent suction control method according to claim 5, wherein The reagent suction control method further comprises: After the reagent suction action is performed once for each hole position in the reagent kit, the actual use number of the corresponding hole position is reduced by 1, and when the actual use number of the hole position is 0, the hole position is marked as an invalid hole position; after all the hole positions in the reagent kit are marked as invalid hole positions, the reagent kit is marked as an invalid reagent kit.

7. A reagent suction control device for application to addition of a reagent in a multiple light emitting system, characterized by, The reagent suction control method further comprises: The receiving module is configured to receive a reagent adding instruction and determine whether an admission variable meets a reagent adding condition; The determining module is configured to determine whether the target reagent needs to be added when the reagent adding condition is met; The execution module is configured to execute an adding process of the target reagent when the target reagent needs to be added; The setting module is configured to set the admission variable as the target reagent suction completion when the target reagent does not need to be added. The reagent suction control method further comprises: The reagent suction control method further comprises: The reagent suction control method further comprises: The reagent suction control method further comprises: The reagent suction control method further comprises: acquiring the actual liquid level of each hole position of the target reagent kit, calculating the actual use number of each hole position, and taking the minimum value of the actual use number of each hole position in the target reagent kit as the first actual use number of the target reagent kit. The reagent suction control system comprises a memory and a processor, the memory stores a reagent suction control program, and the processor runs the reagent suction control program to enable the reagent suction control system to perform the reagent suction control method according to any one of claims 1-6. The computer readable storage medium stores a reagent suction control program, and the reagent suction control program is executed by a processor to implement the reagent suction control method according to any one of claims 1-6.

8. A reagent suction control system characterized by comprising: ​ 9. A computer-readable storage medium, characterized in that, ​

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