A system and method for collecting crude urokinase

Abstract

The application discloses a urokinase crude extract collecting system and method. In the system, a buffer tank is communicated with each collecting area and a circulating tank. The inner cavity of the circulating tank is sequentially divided into a first inner cavity, a second inner cavity and a third inner cavity by two screen plates. An automatic feeding device is arranged above the circulating tank. A first liquid inlet branch pipe is arranged in the first inner cavity and communicated with a pump inlet pipe of a first water pump. A second liquid inlet branch pipe is arranged in the second inner cavity and communicated with the collecting tank. A third liquid inlet branch pipe is arranged in the third inner cavity and communicated with the pump inlet pipe of the first water pump. The pump outlet pipe of the first water pump is communicated with the first liquid inlet branch pipe, the second liquid inlet branch pipe and the third liquid inlet branch pipe. A filtering device is arranged in the collecting tank and communicated with the pump inlet pipe of the first water pump. The pump outlet pipe of the first water pump is communicated with a discharge pipe. Compared with the prior art, the application can realize urine collecting, external cleaning, urokinase adsorption and internal cleaning, and realize automatic collection of urokinase crude extract.

Description

Technical Field

[0001] This invention relates to the field of urokinase collection equipment technology, and in particular to a system and method for collecting crude urokinase extract. Background Technology

[0002] Urokinase is an alkaline protease extracted from urine, produced by the human kidneys, and mainly found in the urine of humans and other mammals. The human body contains a substance called fibrin, which can form blood clots. In patients with thrombosis, blood clots form in the blood vessels, obstructing blood flow and causing serious consequences. A substance called plasmin can degrade fibrin, and urokinase can activate inactive plasminogen activator into plasmin. This is where thrombolytic drugs are needed.

[0003] Urokinase is mainly extracted from human urine, but the content of urokinase in urine is relatively low. According to current technology, about 120-150g of urokinase can be extracted from one ton of urine. There are three main methods for urokinase extraction:

[0004] 1. Foaming method: The urine is rapidly stirred by a machine to make it foam, and then ammonium sulfate (NH4)2SO4 is added to precipitate urokinase to obtain crude product; 2. Precipitating agent method: A precipitating agent is added to the urine to precipitate urokinase; 3. Adsorbent method: An adsorbent is added to the urine to adsorb urokinase.

[0005] Currently, the adsorption method is the most widely used in the urokinase extraction process, and the specific process flow is as follows:

[0006] S1. Collect male urine from public toilets in schools, factories, etc. The freshness of the urine affects the activity of urokinase, so it must be processed promptly. Collect and process the urine from toilets at regular intervals every day. Adjust the pH of the urine to 9, let it stand for 1 hour, siphon the supernatant, and discard the grayish-white precipitate. S2. Adsorption: Adjust the pH of the siphoned supernatant to 5.8-6 with 5N hydrochloric acid (HCl), add 1% (WIV) resin, stir and adsorb for 1 hour, stop stirring, let the resin settle naturally, siphon to remove residual urine, and collect the resin. Wash the collected resin three times with tap water, and then three times with deionized water until the urine is colorless. S3. Elution: For the first elution, use 2% ammonium hydroxide and 1M sodium chloride eluent at 35% volume and stir for 1 hour. For the second elution, use the same eluent and the same volume for 30 minutes, and start collecting urokinase. S4. After the finished urokinase is sent to a testing unit to test its activity, it can be sold to crude product purchasers or biochemical pharmaceutical companies.

[0007] In the above-mentioned urokinase extraction process, each step is carried out manually, especially urine collection and adsorption, which are inefficient. In the adsorption process of some existing extraction equipment, the adsorbent material tends to settle at the bottom of the urine, which limits the adsorption effect of the adsorbent material on urokinase. Summary of the Invention

[0008] The purpose of this invention is to provide a system and method for collecting crude urokinase extract, which addresses the current state of the technology and enables the automated collection of crude urokinase extract by completing urine collection, external cleaning, urokinase adsorption, and internal cleaning.

[0009] To achieve the above objectives, the present invention adopts the following technical solution:

[0010] A crude urokinase extract collection system includes a buffer tank, a circulation tank, a first water pump, a collection tank, a discharge pipe, and several collection zones;

[0011] The buffer tank is connected to each collection area through a first inlet pipe and is lower than each collection area. The buffer tank is connected to the circulation tank through a first outlet pipe. A first electric valve is installed on the first outlet pipe and the circulation tank is lower than the buffer tank. A first liquid level sensor is installed on the buffer tank to monitor the liquid level inside the buffer tank.

[0012] The inner cavity of the circulation tank is divided into a first inner cavity, a second inner cavity, and a third inner cavity by two screen plates. An automatic feeding device is provided above the circulation tank for feeding adsorbent material into the second inner cavity of the circulation tank. A second liquid level sensor is provided on the circulation tank for monitoring the liquid level in the circulation tank.

[0013] The first inner cavity is provided with a first liquid inlet branch pipe, and a second electric valve is provided on the first liquid inlet branch pipe. The first inner cavity is connected to the pump inlet pipe of the first water pump through a first liquid outlet branch pipe, and a third electric valve is provided on the first liquid outlet branch pipe.

[0014] The second inner cavity is provided with a second inlet branch pipe, and a fourth electric valve is provided on the second inlet branch pipe. The second inner cavity is connected to the collection box through a second drain branch pipe, and a fifth electric valve is provided on the second drain branch pipe. The collection box is lower than the circulation box.

[0015] The third inner cavity is provided with a third liquid inlet branch pipe, and a sixth electric valve is provided on the third liquid inlet branch pipe. The third inner cavity is connected to the pump inlet pipe of the first water pump through the third liquid outlet branch pipe, and a seventh electric valve is provided on the third liquid outlet branch pipe.

[0016] The pump outlet pipe of the first water pump is connected to the first inlet branch pipe, the second inlet branch pipe, and the third inlet branch pipe;

[0017] The collection box is equipped with a filtration device that separates the liquid discharged from the second inner cavity into the collection box from the adsorbent material. The collection box is connected to the pump inlet pipe of the first water pump through the fourth drain pipe. The fourth drain pipe is equipped with a first check valve. The pump outlet pipe of the first water pump is connected to the discharge pipe through the second drain pipe. The second drain pipe is equipped with an eighth electric valve.

[0018] Furthermore, each collection area is connected to a first cleaning branch pipe, and each first cleaning branch pipe is equipped with a ninth electric valve. The buffer tank is equipped with a second cleaning branch pipe, and the second cleaning branch pipe is equipped with a tenth electric valve. The outlet of the second cleaning branch pipe is connected to a nozzle. The pump inlet pipe of the first water pump is connected to a water source through a cleaning inlet pipe, and the cleaning inlet pipe is equipped with an eleventh electric valve. The pump outlet pipe of the first water pump is connected to the first cleaning branch pipe of each collection area and the second cleaning branch pipe of the buffer tank through a cleaning outlet pipe.

[0019] Furthermore, it also includes a cleaning tank for holding cleaning agents. The cleaning tank is connected to the pump outlet pipe or cleaning water outlet pipe of the first water pump through a cleaning agent pipeline. Starting from the cleaning tank, a second water pump and a second one-way valve are sequentially installed on the cleaning agent pipeline.

[0020] Furthermore, the filtration device is a filter bag fitted onto the end of the second drain branch pipe that connects to the collection box.

[0021] Furthermore, the outlets of the first, second, and third inlet branches are all connected to nozzles. The nozzle on the first inlet branch is located in the upper half of the first inner cavity, the nozzle on the second inlet branch is located in the lower half of the second inner cavity, and the nozzle on the third inlet branch is located in the upper half of the third inner cavity.

[0022] Furthermore, the first inlet pipe is connected to the outlet pipe via a three-way electric valve.

[0023] Furthermore, the buffer tank is connected to the discharge pipe via a third drain pipe, and a thirteenth electric valve is installed on the third drain pipe.

[0024] A method for collecting crude urokinase extract, using the aforementioned crude urokinase extract collection system, includes the following steps:

[0025] Urine collection: Collect urine from each collection area into a buffer tank;

[0026] External cleaning: The first water pump injects cleaning water into each collection area in sequence to clean each collection area. The wastewater after cleaning is discharged through the discharge pipe.

[0027] Urokinase adsorption: After external cleaning, urine from the buffer tank is injected into the circulation tank; then, adsorbent material is added to the second inner cavity of the circulation tank through an automatic feeding device. At the same time, the following two steps are performed alternately according to the first preset time: urine from the first inner cavity is injected into the second and third inner cavities through the first water pump, and urine from the third inner cavity is injected into the first and second inner cavities through the first water pump; finally, urine from the circulation tank is injected into the collection tank, and the urine is discharged through the discharge pipe, while the adsorbent material remains in the collection tank.

[0028] Internal cleaning: After urokinase adsorption is complete, cleaning water is injected into the buffer tank by the first water pump to clean the buffer tank; after the buffer tank is cleaned, the cleaning water in the buffer tank is injected into the circulation tank. At the same time, the following two steps are alternately performed according to the second preset time: the cleaning water in the first inner cavity is injected into the second and third inner cavities by the first water pump, and the cleaning water in the third inner cavity is injected into the first and second inner cavities by the first water pump to clean the circulation tank; after the circulation tank is cleaned, the cleaning water in the circulation tank is injected into the collection tank to clean the collection tank. During the cleaning of the collection tank, the cleaned wastewater is discharged through the discharge pipe.

[0029] Furthermore, in the urokinase adsorption step, before the urine is discharged through the discharge pipe, the following steps are also included: injecting the urine in the circulation tank into the collection tank, and simultaneously injecting the urine in the collection tank into the first inner cavity and the third inner cavity through the first water pump, so as to wash away the adsorbent material remaining on the two screen plates in the circulation tank.

[0030] Furthermore, before urine collection, external cleaning, urokinase adsorption, and internal cleaning, the following steps are also included:

[0031] External deep cleaning includes the following steps: the first water pump injects cleaning water into each collection area in sequence, and the second water pump adds cleaning agent to the cleaning water to perform deep cleaning of each collection area. The wastewater after cleaning is discharged through the discharge pipe.

[0032] After the external deep cleaning is completed, perform at least one external cleaning cycle.

[0033] The internal deep cleaning process includes the following steps: First, a first water pump injects cleaning water into a buffer tank, and a second water pump adds cleaning agent to the cleaning water to deeply clean the buffer tank. After the buffer tank is deeply cleaned, the cleaning water in the buffer tank is injected into a circulation tank. Simultaneously, the following two steps are performed alternately at a third preset time: First, a first water pump injects cleaning water from the first inner cavity into the second and third inner cavities; second, a first water pump injects cleaning water from the third inner cavity into the first and second inner cavities to deeply clean the circulation tank. After the circulation tank is deeply cleaned, the cleaning water in the circulation tank is injected into a collection tank to deeply clean the collection tank. During the deep cleaning of the collection tank, the cleaned wastewater is discharged through a discharge pipe.

[0034] After the internal deep cleaning is completed, perform at least one internal cleaning.

[0035] The beneficial effects of this invention are as follows:

[0036] This invention can complete urine collection, external cleaning, urokinase adsorption and internal cleaning, realizing the automated collection of crude urokinase extract. Moreover, during the urokinase adsorption process, based on the multi-chamber structure of the circulation box and the alternating circulation mode, it can ensure that the adsorbent material is always suspended in the urine, thereby improving the adsorption rate. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the urokinase crude extract collection system of the present invention;

[0038] Figure 2 This is a schematic diagram of the structure of the buffer tank of the present invention;

[0039] Figure 3 This is a schematic diagram of the structure of the circulation box of the present invention;

[0040] Figure 4 This is a schematic diagram of the structure of the collection box of the present invention.

[0041] Labeling Explanation: 1. Ninth Electric Valve, 2. First Cleaning Branch Pipe, 3. Collection Area, 4. First Inlet Pipe, 5. Three-Way Electric Valve, 6. Fifteenth Electric Valve, 7. Buffer Tank, 8. Overflow Branch Pipe, 9. Cleaning Outlet Pipe, 10. Circulation Tank, 10-1. First Inner Chamber, 10-2. Second Inner Chamber, 10-3. Third Inner Chamber, 11. Cleaning Tank, 12. Second Water Pump, 13. Second Check Valve, 14. Collection Tank, 15. First Water Pump, 16. Discharge Pipe, 17. Fourteenth Electric Valve, 18. Eighth Electric Valve, 19. Second Drain Pipe, 20. Cleaning Inlet Pipe, 21. Eleventh Electric Valve, 22. 23. Second cleaning branch pipe, 24. Tenth electric valve, 25. Nozzle, 26. First drain pipe, 27. First electric valve, 28. Third drain pipe, 29. Thirteenth electric valve, 30. First inlet branch pipe, 31. Second inlet branch pipe, 32. Fourth electric valve, 33. Third inlet branch pipe, 34. Sixth electric valve, 35. First drain branch pipe, 36. Third electric valve, 37. Second drain branch pipe, 38. Fifth electric valve, 39. Third drain branch pipe, 40. Seventh electric valve, 41. Screen plate, 42. Filter device, 43. Fourth drain pipe, 44. First check valve. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific examples described herein are merely illustrative and not intended to limit the scope of the invention.

[0043] Please see Figure 1-4 As shown, a crude urokinase extract collection system includes a buffer tank 7, a circulation tank 10, a first water pump 15, a collection tank 14, a discharge pipe 16, and several collection areas 3, where the collection area 3 refers to a urinal or other device that can collect urine.

[0044] The buffer tank 7 is connected to each collection zone 3 via a first inlet pipe 4, and the buffer tank 7 is lower than each collection zone 3, allowing urine in the collection zone 3 to flow into the buffer tank 7 by gravity. The buffer tank 7 is connected to the circulation tank 10 via a first drain pipe 25, and a first electric valve 26 is installed on the first drain pipe 25 to control the opening and closing of the first drain pipe 25. The circulation tank 10 is lower than the buffer tank 7, allowing urine in the buffer tank 7 to flow into the circulation tank 10 by gravity. The buffer tank 7 is equipped with a first liquid level sensor to monitor the liquid level inside the buffer tank 7.

[0045] The first inlet pipe 4 is connected to the outlet pipe 16 via a three-way electric valve 5. The three-way electric valve 5 allows the collection area 3 to be connected to the buffer tank 7 or the outlet pipe 16. The buffer tank 7 is connected to the outlet pipe 16 via a third outlet pipe 27. A thirteenth electric valve 28 is installed on the third outlet pipe 27 to control its opening and closing. Opening the first electric valve 26 and closing the thirteenth electric valve 28 connects the buffer tank 7 to the circulation tank 10, while closing the first electric valve 26 and opening the thirteenth electric valve 28 connects the buffer tank 7 to the outlet pipe 16.

[0046] The inner cavity of the circulation tank 10 is sequentially divided into a first inner cavity 10-1, a second inner cavity 10-2, and a third inner cavity 10-3 by two screen plates 41. An automatic feeding device is provided above the circulation tank 10 for adding adsorbent material to the second inner cavity 10-2 of the circulation tank 10. Common adsorbent materials include silica gel particles. A second liquid level sensor is provided on the circulation tank 10 for monitoring the liquid level inside the circulation tank 10.

[0047] It should be noted that since the inner cavity of the circulation tank 10 is divided into a first inner cavity 10-1, a second inner cavity 10-2, and a third inner cavity 10-3 by two screen plates 41, the first inner cavity 10-1, the second inner cavity 10-2, and the third inner cavity 10-3 are communicating vessels. Therefore, the buffer tank 7 can be connected to any inner cavity of the circulation tank 10 through the first drain pipe 25. Figure 1 In this embodiment, the buffer tank 7 is connected to the first inner cavity 10-1 of the circulation tank 10 through the first drain pipe 25.

[0048] The first inner cavity 10-1 is provided with a first inlet branch pipe 29, and a second electric valve 30 is provided on the first inlet branch pipe 29 for controlling the opening and closing of the first inlet branch pipe 29. The first inner cavity 10-1 is connected to the pump inlet pipe of the first water pump 15 through a first drain branch pipe 35, and a third electric valve 36 is provided on the first drain branch pipe 35 for controlling the opening and closing of the first drain branch pipe 35.

[0049] The second inner cavity 10-2 is provided with a second inlet branch pipe 31, and a fourth electric valve 32 is provided on the second inlet branch pipe 31 to control the opening and closing of the second inlet branch pipe 31. The second inner cavity 10-2 is connected to the collection box 14 through a second drain branch pipe 37, and a fifth electric valve 38 is provided on the second drain branch pipe 37 to control the opening and closing of the second drain branch pipe 37. The collection box 14 is lower than the circulation box 10, so that the urine in the circulation box 10 can flow into the collection box 14 by gravity.

[0050] The third inner cavity 10-3 is provided with a third inlet branch pipe 33, and a sixth electric valve 34 is provided on the third inlet branch pipe 33 for controlling the opening and closing of the third inlet branch pipe 33. The third inner cavity 10-2 is connected to the pump inlet pipe of the first water pump 15 through the third drain branch pipe 39, and a seventh electric valve 40 is provided on the third drain branch pipe 39 for controlling the opening and closing of the third drain branch pipe 39.

[0051] Necessarily, the outlets of the first liquid inlet branch pipe 29, the second liquid inlet branch pipe 31, and the third liquid inlet branch pipe 33 are all connected to the nozzle 24. The nozzle 24 on the first liquid inlet branch pipe 29 is located in the upper half of the first inner cavity 10-1, the nozzle 24 on the second liquid inlet branch pipe 31 is located in the lower half of the second inner cavity 10-2, and the nozzle 24 on the third liquid inlet branch pipe 33 is located in the upper half of the third inner cavity 10-3.

[0052] According to the above design, when the liquid in the first liquid inlet branch pipe 29 and the third liquid inlet branch pipe 33 is sprayed out through the nozzle, it is done alternately. The purpose is twofold: first, to make the liquid flow, and second, to impact the two screen plates 41 in the circulation tank 10, thereby washing down the adsorbent material attached to the screen surface of the screen plate 41 in the second chamber 10-2, so as to accelerate the flow of the liquid. When the liquid in the second liquid inlet branch pipe 31 is sprayed out through the nozzle 24, it will mainly impact the adsorbent material at the bottom of the second inner chamber 10-2 to prevent the adsorbent material from sinking to the bottom.

[0053] The pump outlet pipe of the first water pump 15 is connected to the first liquid inlet branch pipe 29, the second liquid inlet branch pipe 31, and the third liquid inlet branch pipe 33.

[0054] The collection tank 14 is equipped with a filter device 42 that separates the liquid discharged from the second inner cavity 10-2 into the collection tank 14 from the adsorbent material. The collection tank 14 is connected to the pump inlet pipe of the first water pump 15 through the fourth drain pipe 43. The fourth drain pipe 43 is equipped with a first one-way valve 44 (so that the liquid in the collection tank can only go out and not in). The pump outlet pipe of the first water pump 15 is connected to the discharge pipe 16 through the second drain pipe 19. The second drain pipe 19 is equipped with an eighth electric valve 18 for controlling the opening and closing of the second drain pipe 19.

[0055] Preferably, the filtration device 42 is a filter bag fitted on the end of the second drain branch pipe 37 that connects to the collection box 14, which facilitates the removal of the separated adsorbent material.

[0056] In the above technical solution, in order to prevent the liquid in the buffer tank 7, circulation tank 10 and collection tank 14 from overflowing, the buffer tank 7, circulation tank 10 and collection tank 14 are all provided with overflow branch pipes 8, and the overflow branch pipes 8 on the buffer tank 7, circulation tank 10 and collection tank 14 are all connected to the discharge pipe 16.

[0057] In one embodiment, each collection zone 3 is connected to a first cleaning branch pipe 2. Each first cleaning branch pipe 2 is equipped with a ninth electric valve 1 for controlling the opening and closing of the first cleaning branch pipe 2. A buffer tank 7 is equipped with a second cleaning branch pipe 22. The second cleaning branch pipe 22 is equipped with a tenth electric valve 23 for controlling the opening and closing of the second cleaning branch pipe 22. The outlet of the second cleaning branch pipe 22 is connected to a nozzle 24. The pump inlet pipe of the first water pump 15 is connected to a water source through a cleaning water inlet pipe 20. The cleaning water inlet pipe 20 is equipped with an eleventh electric valve 21 for controlling the opening and closing of the cleaning water inlet pipe 20. The pump outlet pipe of the first water pump 15 is connected to the first cleaning branch pipe 2 of each collection zone 3 and the second cleaning branch pipe 22 of the buffer tank 7 through a cleaning water outlet pipe 9.

[0058] According to the above implementation method, the pump outlet pipe of the first water pump 15 is first divided into two paths, one going to the circulation tank 10, and the other path is further divided into three paths, one going to the discharge pipe 16, one going to the collection area 3, and one going to the buffer tank 7. Therefore, in the actual pipeline design, it is necessary to install a fourteenth electric valve 17 on the pump outlet pipe of the first water pump 15 for overall control of the second drain pipe 19 and the cleaning water outlet pipe 9; and to install a fifteenth electric valve 6 at the end of the cleaning water outlet pipe 9 near the first cleaning branch pipe 1 for overall control of each of the first cleaning branch pipes 1.

[0059] Preferably, it also includes a cleaning tank 11 for holding cleaning agent. The cleaning tank 11 is connected to the pump outlet pipe of the first water pump 15 or the cleaning water outlet pipe 9 via a cleaning agent pipeline. Starting from the cleaning tank 11, a second water pump 12 and a second one-way valve 13 are sequentially installed on the cleaning agent pipeline (to ensure that the liquid in the cleaning tank only flows out and not in). Figure 1In this embodiment, the cleaning tank 11 is connected to the pump outlet pipe of the first water pump 15 through a cleaning agent pipeline.

[0060] Specifically, a method for collecting crude urokinase extract, using the aforementioned crude urokinase extract collection system, includes the following steps:

[0061] Urine collection: The urine from each collection zone 3 is collected into the buffer tank 7 (flow direction: collection zone 3 → first inlet pipe 4 → buffer tank 7, and the relevant valves are switched on and off according to the flow direction, the same below);

[0062] External cleaning: The first water pump 15 injects cleaning water into each collection area 3 in sequence (flow direction: water source → cleaning inlet pipe 20 → first water pump 15 → cleaning outlet pipe 9 → first cleaning branch pipe 2 → collection area 3) to clean each collection area 3. The wastewater after cleaning is discharged through the discharge pipe 16 (flow direction: collection area 3 → first liquid inlet pipe 4 → discharge pipe 16).

[0063] Urokinase Adsorption: After external cleaning, the urine in the buffer tank 7 is injected into the circulation tank 10 (flow direction: buffer tank 7 → first drain pipe 25 → circulation tank 10); then, adsorption material is added to the second inner cavity 10-2 of the circulation tank through an automatic feeding device. At the same time, the following two steps are performed alternately according to the first preset time: the urine in the first inner cavity 10-1 is injected into the second inner cavity 10-2 and the third inner cavity 10-3 through the first water pump 15 (flow direction: first inner cavity 10-1 → first drain branch pipe 35 → first water pump 15 → second inlet branch pipe 31 → second inner cavity 10-2 and first inner cavity 10-1 → first drain branch pipe 35 → first water pump 15 → third inlet branch pipe 33 → third inner cavity 10-3), and the urine in the first inner cavity 10-1 is injected into the second inner cavity 10-2 and the third inner cavity 10-3 through the first water pump 15. Pump 15 injects urine from the third inner cavity 10-3 into the first inner cavity 10-1 and the second inner cavity 10-2 (flow direction: third inner cavity 10-3 → third drain branch pipe 39 → first water pump 15 → first inlet branch pipe 29 → first inner cavity 10-1 and third inner cavity 10-3 → third drain branch pipe 39 → first water pump 15 → second inlet branch pipe 31 → second inner cavity 10-2); finally, the urine in the circulation tank 10 is injected into the collection tank 14, and the urine is discharged through the discharge pipe 16. The adsorbent material will remain in the collection tank 14 (flow direction: second inner cavity 10-2 → second drain branch pipe 37 → collection tank 14 → fourth drain pipe 43 → first water pump 15 → second drain pipe 19 → discharge pipe 16, the first water pump 15 does not need to be turned on).

[0064] Internal cleaning: After urokinase adsorption is completed, cleaning water is injected into buffer tank 7 by the first water pump 15 (flow direction: water source → cleaning inlet pipe 20 → first water pump 15 → cleaning outlet pipe 9 → second cleaning branch pipe 22 → buffer tank 7) to clean buffer tank 7; after buffer tank 7 is cleaned, the cleaning water in buffer tank 7 is injected into circulation tank 10. At the same time, the following two steps are alternately performed according to the second preset time: the cleaning water in the first inner cavity 10-1 is injected into the second inner cavity 10-2 and the third inner cavity 10-3 by the first water pump 15, and the cleaning water in the third inner cavity 10-3 is injected into the first inner cavity 10-1 and the second inner cavity 10-2 by the first water pump 15 to clean circulation tank 10; after circulation tank 10 is cleaned, the cleaning water in circulation tank 10 is injected into collection tank 14 to clean collection tank 14. During the cleaning of collection tank 14, the cleaned wastewater is discharged through discharge pipe 16.

[0065] After internal cleaning is completed, wait for urine collection, external cleaning, urokinase adsorption, and internal cleaning to be performed again.

[0066] The first liquid level sensor is used to monitor the liquid level in the buffer tank 7. For example, when the liquid level is detected to be sufficient for production, the urine in the buffer tank 7 can be allowed to enter the circulation tank 10. The second liquid level sensor is used to monitor the liquid level in the circulation tank 10. For example, when the liquid level is detected to be sufficient for production, the urine in the buffer tank 7 can be prevented from entering the circulation tank 10.

[0067] In the above technical solution, during the urokinase adsorption process, the first water pump 15 injects urine from the first inner cavity 10-1 into the second inner cavity 10-2 and the third inner cavity 10-3, and the first water pump 15 injects urine from the third inner cavity 10-3 into the first inner cavity 10-1 and the second inner cavity 10-2. This cycle continuously injects urine into the second inner cavity 10-2 to disperse the adsorbent material and ensure that the adsorbent material is always suspended in the urine in the second inner cavity 10-2, but the liquid level in the second inner cavity 10-2 will not continuously rise.

[0068] The principle is that the three chambers are separated by two screen plates 41. When entering the production step, the screen surfaces on both sides of the second inner chamber 10-2 will be blocked by the adsorbent material in the chamber, resulting in poor liquid flow in the three chambers and creating a liquid level difference. When urine is injected from the first inner chamber 10-1 into the third inner chamber 10-3, the urine in the third inner chamber 10-3 will slowly flow through the screen plate 41 to the second inner chamber 10-2. The water flow will remove the urine adhering to the space between the second inner chamber 10-2 and the third inner chamber 10-3. The adsorbent material on the screen plate 41 is washed down, while urine in the second inner cavity 10-2 slowly flows through the screen plate 41 to the first inner cavity 10-1. Meanwhile, the adsorbent material in the second inner cavity 10-2 gradually adheres to the screen plate 41 between the second inner cavity 10-2 and the first inner cavity 10-1. Therefore, the flow rate of urine from the third inner cavity 10-3 to the second inner cavity 10-2 is greater than the flow rate from the second inner cavity 10-2 to the first inner cavity 10-1. Conversely, when urine is injected from the third inner cavity 10-3 into the first inner cavity 10-1, the flow rate of urine from the first inner cavity 10-1 to the second inner cavity 10-2 is greater than the flow rate from the second inner cavity to the third inner cavity 10-3. Therefore, the urine level in the second inner cavity 10-2 undergoes a continuous rise and fall during the urokinase adsorption process, which is why it does not continuously rise and overflow.

[0069] Preferably, in the urokinase adsorption step, before the urine is discharged through the discharge pipe 16, the following steps are also included: injecting the urine in the circulation tank 10 into the collection tank 14, and simultaneously injecting the urine in the collection tank 14 into the first inner cavity 10-1 and the third inner cavity 10-3 through the first water pump 15, so as to wash away the adsorbent material remaining on the two screen plates 41 in the circulation tank 10 (second inner cavity 10-2 → second drain branch pipe 37 → collection tank 14 → fourth drain pipe 43 → first water pump 15 → first inlet branch pipe 29 → first inner cavity 10-1 and second inner cavity 10-2 → second drain branch pipe 37 → collection tank 14 → fourth drain pipe 43 → first water pump 15 → third inlet branch pipe 33 → third inner cavity 10-3).

[0070] Similarly, after the urokinase adsorption step is completed, the first water pump 15 injects cleaning water into the first inner cavity 10-1 and the third inner cavity 10-3, which can also wash away the residual adsorbent material on the two screen plates 41 in the circulation tank 10 (flow direction: water source → cleaning inlet pipe 20 → first water pump 15 → first liquid inlet branch pipe 29 → first inner cavity 10-1 and water source → cleaning inlet pipe 20 → first water pump 15 → third liquid inlet branch pipe 33 → third inner cavity 10-3). Then, the fifth electric valve 38 is opened to discharge the cleaning water and the washed-away adsorbent material into the collection tank 14. The adsorbent material remains in the collection tank 14, and the wastewater after cleaning is discharged through the discharge pipe 16 (flow direction: second inner cavity 10-2 → second discharge branch pipe 37 → collection tank 14 → fourth discharge pipe 43 → first water pump 15 → second discharge pipe 19 → discharge pipe 16. The first water pump 15 does not need to be turned on).

[0071] The above technical solution, before urine collection, external cleaning, urokinase adsorption, and internal cleaning, also includes the following steps:

[0072] External deep cleaning includes the following steps: the first water pump 15 injects cleaning water into each collection area in sequence, and the second water pump 12 adds cleaning agent to the cleaning water to perform deep cleaning on each collection area 3. The wastewater after cleaning is discharged through the discharge pipe 16.

[0073] After the external deep cleaning is completed, perform at least one more external cleaning to avoid residual cleaning agent affecting subsequent collection.

[0074] The internal deep cleaning includes the following steps: First water pump 15 injects cleaning water into buffer tank 7, and second water pump 12 adds cleaning agent to the cleaning water (flow direction: cleaning tank 11 → cleaning agent pipeline → pump outlet pipe of first water pump 15 or cleaning water outlet pipe 9), deeply cleaning buffer tank 7; after deep cleaning of buffer tank 7, the cleaning water in buffer tank 7 is injected into circulation tank 10. Simultaneously, the following two steps are performed alternately according to a third preset time: first water pump 15 injects cleaning water from first inner cavity 10-1 into second inner cavity 10-2 and third inner cavity 10-3; first water pump 15 injects cleaning water from third inner cavity 10-3 into first inner cavity 10-1 and second inner cavity 10-2, deeply cleaning circulation tank 10; after deep cleaning of circulation tank 10, the cleaning water in circulation tank 10 is injected into collection tank 14, deeply cleaning collection tank 14. During the deep cleaning of collection tank 14, the cleaned wastewater is discharged through discharge pipe 16.

[0075] After the internal deep cleaning is completed, perform at least one internal cleaning to avoid residual cleaning agent affecting subsequent collection.

[0076] The aforementioned external and internal deep cleaning is generally performed late at night.

[0077] In addition, the first, second, and third preset times are set according to the actual production situation; each valve and pump is controlled by a controller, and the control logic is set according to production needs.

[0078] In summary, this invention can complete urine collection, external cleaning, urokinase adsorption, and internal cleaning, achieving automated collection of crude urokinase extract. Moreover, during the urokinase adsorption process, based on the multi-chamber structure of the circulation box 10 and the alternating circulation mode, it can ensure that the adsorbent material is always suspended in the urine, thereby improving the adsorption rate.

[0079] This invention is not limited to the specific embodiments described above. Those skilled in the art can implement this invention using various other specific embodiments based on the content disclosed herein. Therefore, any design that adopts the design structure and concept of this invention and makes some simple changes or modifications falls within the scope of protection of this invention.

Claims

1. A system for collecting crude urokinase extract, characterized in that: It includes a buffer tank, a circulation tank, a first water pump, a collection tank, a discharge pipe, and several collection areas; The buffer tank is connected to each collection area through a first inlet pipe and is lower than each collection area. The buffer tank is connected to the circulation tank through a first outlet pipe. A first electric valve is installed on the first outlet pipe and the circulation tank is lower than the buffer tank. A first liquid level sensor is installed on the buffer tank to monitor the liquid level inside the buffer tank. The inner cavity of the circulation tank is divided into a first inner cavity, a second inner cavity, and a third inner cavity by two screen plates. An automatic feeding device is provided above the circulation tank for feeding adsorbent material into the second inner cavity of the circulation tank. A second liquid level sensor is provided on the circulation tank for monitoring the liquid level in the circulation tank. The first inner cavity is provided with a first liquid inlet branch pipe, and a second electric valve is provided on the first liquid inlet branch pipe. The first inner cavity is connected to the pump inlet pipe of the first water pump through a first liquid outlet branch pipe, and a third electric valve is provided on the first liquid outlet branch pipe. The second inner cavity is provided with a second inlet branch pipe, and a fourth electric valve is provided on the second inlet branch pipe. The second inner cavity is connected to the collection box through a second drain branch pipe, and a fifth electric valve is provided on the second drain branch pipe. The collection box is lower than the circulation box. The third inner cavity is provided with a third liquid inlet branch pipe, and a sixth electric valve is provided on the third liquid inlet branch pipe. The third inner cavity is connected to the pump inlet pipe of the first water pump through the third liquid outlet branch pipe, and a seventh electric valve is provided on the third liquid outlet branch pipe. The pump outlet pipe of the first water pump is connected to the first inlet branch pipe, the second inlet branch pipe, and the third inlet branch pipe; The collection box is equipped with a filtration device that separates the liquid discharged from the second inner cavity into the collection box from the adsorbent material. The collection box is connected to the pump inlet pipe of the first water pump through the fourth drain pipe. The fourth drain pipe is equipped with a first check valve. The pump outlet pipe of the first water pump is connected to the discharge pipe through the second drain pipe. The second drain pipe is equipped with an eighth electric valve.

2. The urokinase crude extract collection system according to claim 1, characterized in that: Each collection area is connected to a first cleaning branch pipe, and each first cleaning branch pipe is equipped with a ninth electric valve. The buffer tank is equipped with a second cleaning branch pipe, and the second cleaning branch pipe is equipped with a tenth electric valve. The outlet of the second cleaning branch pipe is connected to a nozzle. The pump inlet pipe of the first water pump is connected to a water source through a cleaning inlet pipe, and the cleaning inlet pipe is equipped with an eleventh electric valve. The pump outlet pipe of the first water pump is connected to the first cleaning branch pipe of each collection area and the second cleaning branch pipe of the buffer tank through a cleaning outlet pipe.

3. The urokinase crude extract collection system according to claim 2, characterized in that: It also includes a cleaning tank for holding cleaning agents. The cleaning tank is connected to the pump outlet pipe or cleaning water outlet pipe of the first water pump through a cleaning agent pipeline. Starting from the cleaning tank, a second water pump and a second one-way valve are sequentially installed on the cleaning agent pipeline.

4. The urokinase crude extract collection system according to claim 1, characterized in that: The filtration device is a filter bag fitted onto the end of the second drain branch pipe that connects to the collection box.

5. The urokinase crude extract collection system according to claim 1, characterized in that: The outlets of the first, second, and third inlet branches are all connected to nozzles. The nozzle on the first inlet branch is located in the upper half of the first inner cavity, the nozzle on the second inlet branch is located in the lower half of the second inner cavity, and the nozzle on the third inlet branch is located in the upper half of the third inner cavity.

6. The urokinase crude extract collection system according to claim 1, characterized in that: The first inlet pipe is connected to the outlet pipe via a three-way electric valve.

7. The urokinase crude extract collection system according to claim 1, characterized in that: The buffer tank is connected to the discharge pipe through the third drain pipe, and the third drain pipe is equipped with a thirteenth electric valve.

8. A method for collecting crude urokinase extract, using the crude urokinase extract collection system according to any one of claims 1-7, characterized in that: Includes the following steps: Urine collection: Collect urine from each collection area into a buffer tank; External cleaning: The first water pump injects cleaning water into each collection area in sequence to clean each collection area. The wastewater after cleaning is discharged through the discharge pipe. Urokinase adsorption: After external cleaning, urine from the buffer tank is injected into the circulation tank; then, adsorbent material is added to the second inner cavity of the circulation tank through an automatic feeding device. At the same time, the following two steps are performed alternately according to the first preset time: urine from the first inner cavity is injected into the second and third inner cavities through the first water pump, and urine from the third inner cavity is injected into the first and second inner cavities through the first water pump; finally, urine from the circulation tank is injected into the collection tank, and the urine is discharged through the discharge pipe, while the adsorbent material remains in the collection tank. Internal cleaning: After urokinase adsorption is complete, cleaning water is injected into the buffer tank by the first water pump to clean the buffer tank; after the buffer tank is cleaned, the cleaning water in the buffer tank is injected into the circulation tank. At the same time, the following two steps are alternately performed according to the second preset time: the cleaning water in the first inner cavity is injected into the second and third inner cavities by the first water pump, and the cleaning water in the third inner cavity is injected into the first and second inner cavities by the first water pump to clean the circulation tank; after the circulation tank is cleaned, the cleaning water in the circulation tank is injected into the collection tank to clean the collection tank. During the cleaning of the collection tank, the cleaned wastewater is discharged through the discharge pipe.

9. A method for collecting crude urokinase extract according to claim 8, characterized in that: Before the urine is discharged through the discharge pipe, the urokinase adsorption process includes the following steps: injecting the urine in the circulation tank into the collection tank, and simultaneously injecting the urine in the collection tank into the first inner cavity and the third inner cavity through the first water pump to flush away the adsorption material remaining on the two screen plates in the circulation tank.

10. A method for collecting crude urokinase extract according to claim 8, characterized in that: Before urine collection, external cleaning, urokinase adsorption, and internal cleaning, the following steps are also included: Before external cleaning, deep external cleaning is carried out, including the following steps: the first water pump injects cleaning water into each collection area in sequence, and the second water pump adds cleaning agent to the cleaning water to carry out deep cleaning of each collection area. The wastewater after cleaning is discharged through the discharge pipe. Before internal cleaning, a deep internal cleaning is performed, including the following steps: First, cleaning water is injected into a buffer tank using a first water pump, and second, cleaning agent is added to the cleaning water to deeply clean the buffer tank. After the buffer tank is deeply cleaned, the cleaning water in the buffer tank is injected into a circulation tank. Simultaneously, the following two steps are performed alternately at a third preset time: first, cleaning water from the first inner cavity is injected into the second and third inner cavities using a first water pump; second, cleaning water from the third inner cavity is injected into the first and second inner cavities using a first water pump to deeply clean the circulation tank. After the circulation tank is deeply cleaned, the cleaning water in the circulation tank is injected into a collection tank to deeply clean the collection tank. During the deep cleaning of the collection tank, the cleaned wastewater is discharged through a discharge pipe.

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