A method for cultivating bee pupa cordyceps which can be mass-produced

Abstract

The application discloses a kind of scalable production's bee pupa cordyceps cultivation method, belong to precious edible mushroom artificial cultivation technical field, select frozen honeybee in mature larva period to metamorphosis period each day old pupa body as culture medium main raw material, through normal pressure sterilization or high pressure sterilization production culture medium, using pipette gun injection method, such as every hour inoculation amount is 20 times of prior art, bee pupa cordyceps culture medium dyeing bacteria success rate can reach more than 98%, such as solve the current by hand injection method inoculation speed slow, time-consuming, dyeing bacteria rate poor drawbacks, and dyeing bacteria period time can be shortened by more than one week, bee pupa cordyceps single bottle yield is more than 2 times of prior art, high safety performance, it is favorable to scale production.The bee pupa cordyceps active ingredient cordycepin content is very high, medicinal value is big, development prospect is wide, not only can drive more beekeeping person to make money, also opens another piece of sky of precious medicinal and edible mushroom industrial production.

Description

Technical Field

[0001] This invention relates to a method for cultivating Cordyceps militaris that can be produced on a large scale, belonging to the field of artificial cultivation technology of rare medicinal fungi. Background Technology

[0002] Cordyceps sinensis is a precious traditional Chinese medicine, regarded as a treasure among tonics since ancient times. With the improvement of people's living standards and the enhancement of health awareness, the demand for Cordyceps sinensis has increased dramatically. Due to the dwindling wild resources and high price, in the 1980s, people began to develop Cordyceps militaris as a substitute for Cordyceps sinensis. In 2009, Lu Jiumei and other researchers at Danyang Jiuxiancao Biotechnology Co., Ltd., through continuous experimentation, successfully cultivated bee pupae as the main raw material for Cordyceps sinensis culture (authorization announcement number CN 104054504B).

[0003] Bee pupae, also known as bee embryos or bee larvae, are unprocessed and uncontaminated. They are rich in high-protein, low-fat, contain a small amount of lecithin, and are rich in various vitamins and trace elements. They are considered a delicacy, possessing benefits such as boosting immunity, promoting urination and protecting the kidneys, replenishing blood and energy, and relieving pain. However, due to their high protein content, many people are prone to allergic reactions when consuming them; therefore, beekeepers rarely produce bee pupae on their own initiative.

[0004] This invention employs artificial cultivation techniques for Cordyceps militaris. Under strict control and nurturing of suitable temperature, light, humidity, and oxygen conditions, the highly active nutrients in bee pupae, which are prone to causing allergies, are converted into bioenergy, resulting in a high-quality, medicinal-grade edible fungus, Cordyceps militaris, suitable for all ages. It contains abundant cordycepin, cordycepic acid, adenosine, cordyceps polysaccharides, protein, SOD, and other active ingredients, making it a leader in the field of high-grade edible fungi Cordyceps militaris. It exhibits significant effects in enhancing human immunity, nourishing the lungs and kidneys, preventing cancer and tumors, and prolonging life.

[0005] This invention expands the field of Cordyceps militaris cultivation, providing an innovative method for cultivating Cordyceps militaris and increasing the content of its functional components. Because bee pupae are small and delicate, this invention uses fresh, mature bee pupae as the host, making them susceptible to contamination by other microorganisms during cultivation, leading to blackening and spoilage. Furthermore, this invention employs a manual injection method and ozone sterilization to prepare the culture medium, which is labor-intensive, time-consuming, and has a low contamination rate, thus hindering large-scale production. Summary of the Invention

[0006] This invention provides a method for cultivating bee pupae and cordyceps to address the shortcomings of existing technologies. It uses frozen bee pupae of various ages, from mature larvae to metamorphosis, as the main raw material for the culture medium, ensuring high safety and immunity to environmental factors. The method employs a pipette spraying technique, using either atmospheric or high-pressure sterilization to prepare the culture medium. The number of bee pupae inoculated per hour is 20 times that of existing technologies, the inoculation rate of bee pupae and cordyceps can reach over 98%, the time for the insect bodies to fully develop mycelium is shortened by more than a week, and the yield of bee pupae and cordyceps is more than twice that of existing technologies. This method is suitable for large-scale production and possesses novel inventiveness and broad application value.

[0007] This invention provides a method for cultivating Cordyceps militaris that can be produced on a large scale, comprising the following steps:

[0008] (1) Select bee pupae with strong bee colony strength as the host for the Cordyceps militaris to be cultivated.

[0009] (2) Preferably, the test tube original strains of Cordyceps militaris that have been preserved recently are selected;

[0010] (3) Preparation of liquid Cordyceps militaris inoculum:

[0011] Formula for liquid Cordyceps militaris inoculum: 20g honey, 8g peptone, 0.7g magnesium sulfate, 1.5g potassium dihydrogen phosphate, 1000ml water;

[0012] b. Preparation method: Put the above formula into 1000ml of purified water, mix well, then add egg white and beat, pour into glass measuring cups, sterilize at 0.137MPa and 121℃ for 30-40 minutes, and then let stand for 10-12 hours.

[0013] c. After standing, filter and purify the solution using sixteen layers of sterile gauze, then fill it into containers and continue sterilization for 30-40 minutes.

[0014] d. Expose the selected Cordyceps militaris test tube original culture to light for 24-48 hours to change color, and then inoculate it into the above c. Incubate at 18-24℃ in the dark for 36 hours, and then incubate at 17-24℃ and 155r / min in the dark for 5-7 days. When the liquid culture medium is covered with mycelium, take it out and place it in a refrigerator at 4℃ for later use.

[0015] (4) Preparation of culture medium: Frozen honeybee pupae of various ages from mature larvae to metamorphosis were used as raw materials, and were dispensed into culture bottles, 30g per bottle. The bottles were sealed with high-temperature resistant polypropylene film, sterilized at normal pressure for 240-360 min or at high pressure for 35-45 min, and then cooled and placed in a sterile room for later use;

[0016] (5) Inoculation and culture: The prepared liquid bacterial culture is sprayed into the culture bottle using a pipette. Each bottle is inoculated with 1.5 to 4 mL of liquid bacterial culture. Each culture bottle is sprayed with liquid bacterial culture 1 to 2 times. After sealing, the bottle is placed in a culture room with a temperature of 15 to 23°C and a humidity of 60 to 65% to be cultured in the dark.

[0017] (6) Cultivation in light:

[0018] The controlled conditions for culture room a are: 18-22℃, 600-800 lx, humidity 65%-85%, and continuous incubation in light for 18-40 hours.

[0019] b. After the mycelia on the culture medium turn yellow, use sterile water to remove the mycelia from the surface of the culture medium.

[0020] (7) Grass induction: First, make 3-5 holes in the sealed plastic film with a disposable medical syringe needle as ventilation holes. The control conditions of the incubation room during the grass induction stage are: light time 14-16h, incubation temperature 20-24℃, humidity 65%-85%; dark light at night 6-10h, incubation temperature 15-17℃, temperature difference between light and dark room 7-9℃, light is white light: 800-1000lx; 2-4 days;

[0021] (8) When the fruiting bodies of Cordyceps militaris grow to 0.5-1cm in height, adjust the light intensity to 200-400lx, the culture temperature to 18-22℃, the humidity to 65%-85%, and expose them to light for 12-14 hours a day;

[0022] (9) Harvesting: When the fruiting bodies of Cordyceps militaris grow to 5-8cm, the whole Cordyceps militaris plant can be harvested. After harvesting, it is dried for 1-2 days, and then dried at a temperature of 45-60℃ until the moisture content of the whole Cordyceps militaris plant is below 8%. Then it is stored in a refrigerator and frozen.

[0023] Preferably, in step (1), the selected bee pupae are: frozen bee pupae of various ages from the mature larval stage to the metamorphosis stage;

[0024] Preferably, in step (2), the original Cordyceps militaris test tube culture that has been recently preserved is preferred, and it is exposed to light 48 hours before inoculation, and those with better color change are selected for use;

[0025] Preferably, in step (3)a, pure natural mature honey is selected;

[0026] Preferably, in step (3)b, the formula in step (3)a is placed in 1000ml of purified water, mixed and stirred, then 2 egg whites are added and stirred thoroughly. The mixture is then poured into a 2000ml glass measuring cup, sterilized at 0.137MPa and 121℃ for 30-40 minutes, and then left to stand for 10-12 hours.

[0027] Preferably, in step (3)c, the solution after standing is filtered and purified with sixteen layers of sterile gauze, and then filled into five 500ml Erlenmeyer flasks, and sterilized at 0.137MPa and 121℃ for 30-40 minutes. When cooled to 95-85℃, it is quickly taken out and sent to the inoculation room.

[0028] Preferably, in step (3)d, the preferred Cordyceps militaris test tube culture is exposed to light for 24-48 hours to change color, and the outer wall of the test tube is wiped with 75% alcohol for disinfection. Then, in a sterile room, 3-5 pieces of 0.2×0.2mm mycelial blocks are picked from the test tube with a transplanting spatula and inoculated into step (3)c. The culture is then placed at 18-24℃ in the dark for 36 hours, and then incubated in a shaker at 17-24℃ and 155r / min in the dark for 5-7 days. When the liquid culture medium is covered with mycelium, it is taken out and placed in a refrigerator at 4℃ for later use.

[0029] Preferably, in step (4), bee pupae are used as raw materials and packaged into culture bottles with a height of 10 cm and a diameter of 7 cm, 30 g per bottle. The bottles are sealed with high-temperature resistant polypropylene film, sterilized at normal pressure for 240–360 min or by autoclaving for 35–45 min, and then cooled and placed in a sterile room for later use.

[0030] Preferably, in step (5), the prepared liquid bacterial culture is injected into the culture bottle using a pipette, with 1.5 to 4 mL inoculated into each bottle. The liquid bacterial culture is sprayed into each culture bottle 1 to 2 times. After sealing, the bottle is placed in a culture room with a temperature of 15 to 23°C and a humidity of 60 to 65% and cultured in the dark for 12 to 15 days.

[0031] Preferably, in step (6)a, the controlled conditions of the culture room are: 18-22℃, white light illumination of 600-800 lx, humidity of 65%-85%, and continuous light culture for 18-40 hours until yellow;

[0032] Preferably, in step (6)b, after the mycelium in the culture medium turns yellow, the mycelium on the surface of the culture medium is removed with sterile water, and then the culture medium is sealed and cultured again.

[0033] Preferably, in step (7), 3 to 5 holes are first punched in the sealed plastic film using a disposable medical syringe needle as ventilation holes. The control conditions of the cultivation room during the grass-inducing stage are: light time 14 to 16 hours, cultivation temperature 20 to 24°C, humidity 65% ​​to 85%; dark light at night 6 to 10 hours, cultivation temperature 15 to 17°C, temperature difference between light and dark rooms 7 to 9°C, and appropriate ventilation every day to ensure fresh air. The light is white light: 800 to 1000 lx; ​​2 to 4 days.

[0034] Preferably, in step (8), when the fruiting bodies of Cordyceps militaris grow to 0.5-1 cm in height, the light intensity is adjusted to 200-400 lx, the culture temperature is 18-22℃, the humidity is 65%-85%, the fruiting bodies are exposed to light for 12-14 hours per day, and the culture time is 30-45 days.

[0035] Preferably, in step (9), the dried bee pupa cordyceps is cooled, sorted, packaged, and stored. When sorting, the bee pupa cordyceps is graded and packaged according to its shape, size, and quality. The storage conditions are: storage away from light and temperature below -5℃.

[0036] Beneficial effects

[0037] Due to the adoption of the above technical solutions, the present invention has the following advantages compared with the prior art:

[0038] 1. This invention uses frozen honeybee pupae of various ages from mature larvae to metamorphosis as the main raw material for the culture medium. The culture medium is prepared by sterilization under normal pressure or high pressure, which has high safety performance and is not affected by environmental factors.

[0039] 2. This invention uses a pipette spraying method, which can inoculate 20 times more bee pupae per hour than existing technologies. The inoculation rate of bee pupae and cordyceps can reach more than 98%, the time for the insect bodies to fully develop mycelium is shortened by more than one week, and the yield of bee pupae and cordyceps is more than twice that of existing technologies, which is convenient for large-scale production.

[0040] 3. This invention can help more beekeepers increase their income and become wealthy, accelerate the economic growth of the beekeeping industry, and promote its healthy development.

[0041] The bee industry, hailed as "aerial agriculture" and a "health industry," integrates economic, social, and ecological benefits and is an important component of modern agriculture. Utilizing only natural resources from a hundred flowers, it yields over ten bee products, including honey, royal jelly, propolis, pollen, and beeswax. These products are not only excellent health foods but also important raw materials for traditional Chinese medicine. Furthermore, bee pollination significantly increases crop yields and economic benefits, ensuring food security. my country is a major beekeeping country, and bee products such as honey, bee pollen, and royal jelly contain abundant natural active nutrients. However, the utilization rate of many of these active ingredients is extremely low, resulting in a significant waste of high-quality resources and causing beekeepers to suffer meager incomes or even losses for many years. If each beekeeper raises 100 bee colonies, and each colony produces 2 kilograms of bee pupae per flowering season, then by selecting two flowering seasons a year, approximately 400 kilograms of bee pupae can be produced. If the purchase price of each kilogram of bee pupae is 130 yuan, then each beekeeper can increase their income by about 52,000 yuan per year from bee pupae alone. Therefore, vigorously developing and cultivating bee pupae and cordyceps can not only contribute to human health, but also make an important contribution to the revitalization of agricultural development, since there are 300,000 beekeepers nationwide. Detailed Implementation

[0042] The present invention will be further described in detail below with reference to specific embodiments. It should be understood that these embodiments are used to illustrate the basic principles, main features and advantages of the present invention, and the present invention is not limited to the scope of the following embodiments. The implementation conditions used in the embodiments can be further adjusted according to specific requirements, and the implementation conditions not specified are generally the conditions in conventional experiments.

[0043] Example 1

[0044] (1) Select bee pupae with strong bee colony strength as the host for the Cordyceps militaris to be cultivated.

[0045] (2) Preferably, the test tube original strains of Cordyceps militaris that have been preserved recently are selected;

[0046] (3) Preparation of liquid Cordyceps militaris inoculum:

[0047] Formula for liquid Cordyceps militaris inoculum: 20g honey, 8g peptone, 0.7g magnesium sulfate, 1.5g potassium dihydrogen phosphate, 1000ml water;

[0048] b. Preparation method: Put the above formula into 1000ml of purified water, mix well, then add egg white and beat, pour into glass measuring cups, sterilize at 0.137MPa and 121℃ for 30-40 minutes, and then let stand for 10-12 hours.

[0049] c. After standing, filter and purify the solution using sixteen layers of sterile gauze, then fill it into containers and continue sterilization for 30-40 minutes.

[0050] d. Expose the selected Cordyceps militaris test tube original culture to light for 24-48 hours to change color, and then inoculate it into the above c. Incubate at 18-24℃ in the dark for 24-48 hours, and then incubate at 17-24℃ and 155r / min in the dark for 5-7 days. When the liquid culture medium is covered with mycelium, take it out and place it in a refrigerator at 4℃ for later use.

[0051] (4) Preparation of culture medium: Frozen bee pupae were used as raw material and dispensed into culture bottles, 30g per bottle. The bottles were sealed with high-temperature resistant polypropylene film and sterilized under normal pressure for 240-360 minutes. After cooling, the bottles were placed in a sterile room for later use.

[0052] (5) Inoculation and culture: The prepared liquid bacterial culture is sprayed into the culture bottle using a pipette. Each bottle is inoculated with 1.5 to 4 mL of liquid bacterial culture. Each culture bottle is sprayed with liquid bacterial culture 1 to 2 times. After sealing, the bottle is placed in a culture room with a temperature of 15 to 23°C and a humidity of 60 to 65% to be cultured in the dark.

[0053] (6) Cultivation in light:

[0054] The controlled conditions for culture room a are: 18-22℃, 600-800 lx, humidity 65%-85%, and continuous incubation in light for 18-40 hours.

[0055] b. After the mycelia on the culture medium turn yellow, use sterile water to remove the mycelia from the surface of the culture medium.

[0056] (7) Grass induction: First, make 3-5 holes in the sealed plastic film with a disposable medical syringe needle as ventilation holes. The control conditions of the incubation room during the grass induction stage are: light time 14-16h, incubation temperature 20-24℃, humidity 65%-85%; dark light at night 6-10h, incubation temperature 15-17℃, temperature difference between light and dark room 7-9℃, light is white light: 800-1000lx; 2-4 days;

[0057] (8) When the fruiting bodies of Cordyceps militaris grow to 0.5-1cm in height, adjust the light intensity to 200-400lx, the culture temperature to 18-22℃, the humidity to 65%-85%, and expose them to light for 12-14 hours a day;

[0058] (9) Harvesting: When the fruiting bodies of Cordyceps militaris grow to 5-8cm, the whole Cordyceps militaris plant can be harvested. After harvesting, it is dried for 1-2 days, and then dried at a temperature of 45-60℃ until the moisture content of the whole Cordyceps militaris plant is below 8%. Then it is stored in a refrigerator and frozen.

[0059] Example 2

[0060] (1) Select bee pupae with strong bee colony strength as the host for the Cordyceps militaris to be cultivated.

[0061] (2) Preferably, the test tube original strains of Cordyceps militaris that have been preserved recently are selected;

[0062] (3) Preparation of liquid Cordyceps militaris strain;

[0063] Formula for liquid Cordyceps militaris inoculum: 20g honey, 8g peptone, 0.7g magnesium sulfate, 1.5g potassium dihydrogen phosphate, 1000ml water;

[0064] b. Preparation method: Put the above formula into 1000ml of purified water, mix well, then add egg white and beat, pour into glass measuring cups, sterilize at 0.137MPa and 121℃ for 30-40 minutes, and then let stand for 10-12 hours.

[0065] c. After standing, filter and purify the solution using sixteen layers of sterile gauze, then fill it into containers and continue sterilization for 30-40 minutes.

[0066] d. Expose the selected Cordyceps militaris test tube original culture to light for 24-48 hours to change color, and then inoculate it into the above c. Incubate at 18-24℃ in the dark for 24-48 hours, and then incubate at 17-24℃ and 155r / min in the dark for 5-7 days. When the liquid culture medium is covered with mycelium, take it out and place it in a refrigerator at 4℃ for later use.

[0067] (4) Preparation of culture medium: Frozen bee pupae were used as raw material and dispensed into culture bottles, 30g per bottle. The bottles were sealed with high-temperature resistant polypropylene film, sterilized by autoclaving for 35-45 minutes, and then cooled and placed in a sterile room for later use.

[0068] (5) Inoculation and culture: The prepared liquid bacterial culture is sprayed into the culture bottle using a pipette. Each bottle is inoculated with 1.5 to 4 mL of liquid bacterial culture. Each culture bottle is sprayed with liquid bacterial culture 1 to 2 times. After sealing, the bottle is placed in a culture room with a temperature of 15 to 23°C and a humidity of 60 to 65% to be cultured in the dark.

[0069] (6) Cultivation in light:

[0070] The controlled conditions for culture room a are: 18-22℃, 600-800 lx, humidity 65%-85%, and continuous incubation in light for 18-40 hours.

[0071] b. After the mycelia on the culture medium turn yellow, use sterile water to remove the mycelia from the surface of the culture medium.

[0072] (7) Grass induction: First, make 3-5 holes in the sealed plastic film with a disposable medical syringe needle as ventilation holes. The control conditions of the incubation room during the grass induction stage are: light time 14-16h, incubation temperature 20-24℃, humidity 65%-85%; dark light at night 6-10h, incubation temperature 15-17℃, temperature difference between light and dark room 7-9℃, light is white light: 800-1000lx; 2-4 days;

[0073] (8) When the fruiting bodies of Cordyceps militaris grow to 0.5-1cm in height, adjust the light intensity to 200-400lx, the culture temperature to 18-22℃, the humidity to 65%-85%, and expose them to light for 12-14 hours a day;

[0074] (9) Harvesting: When the fruiting bodies of Cordyceps militaris grow to 5-8cm, the whole Cordyceps militaris plant can be harvested. After harvesting, it is dried for 1-2 days, and then dried at a temperature of 45-60℃ until the moisture content of the whole Cordyceps militaris plant is below 8%. Then it is stored in a refrigerator and frozen.

[0075] Comparative example:

[0076] The key difference from Examples 1-2 is that fresh, mature bee pupae are used as the host, and the culture medium is prepared by ozone sterilization using a manual injection method.

[0077] As shown in the table above, the number of bee pupae inoculated per hour by this invention is 20 times that of the prior art, the inoculation rate of bee pupae and cordyceps can reach more than 95%, the time for the insect body to be fully inoculated is shortened by more than one week, and the yield of bee pupae and cordyceps is more than twice that of the prior art, which is convenient for large-scale production.

[0078] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for cultivating a scalable production of Cordyceps bombycis, characterized by, Includes the following steps: (1) Select bee pupae with strong bee colony strength as the host for the Cordyceps militaris to be cultivated. (2) Select recently preserved Cordyceps militaris test tubes; (3) Preparation of liquid Cordyceps militaris inoculum: Formula for liquid Cordyceps militaris inoculum: 20g honey, 8g peptone, 0.7g magnesium sulfate, 1.5g potassium dihydrogen phosphate, 1000ml water; b. Preparation method: Put the above formula into 1000ml of purified water, mix well, then add egg white and beat, pour into glass measuring cups, sterilize at 0.137MPa and 121℃ for 30-40 minutes, and then let stand for 10-12 hours. c. After standing, filter and purify the solution using sixteen layers of sterile gauze, then fill it into containers and continue sterilization for 30-40 minutes. d. Expose the selected Cordyceps militaris test tubes to light for 24-48 hours until they change color, then inoculate them into the above c. Incubate at 18-24℃ in the dark for 36 hours, then incubate at 17-24℃ and 155r / min in the dark for 5-7 days. When the liquid culture medium is covered with mycelia, remove it and store it in a refrigerator at 4℃ for later use. (4) Preparation of culture medium: Frozen honeybee pupae of various ages from mature larvae to metamorphosis are used as raw materials, and are dispensed into culture bottles, 30g per bottle. Each bottle is sealed with high-temperature resistant polypropylene film and sterilized under normal pressure for 240-360 minutes or under high pressure for 35 minutes. After 45 minutes, cool and store in a sterile room for later use. (5) Inoculation and culture: The prepared liquid bacterial culture is sprayed into the culture bottle using a pipette. Each bottle is inoculated with 1.5 to 4 mL of liquid bacterial culture. Each culture bottle is sprayed with liquid bacterial culture 1 to 2 times. After sealing, the bottle is placed in a culture room with a temperature of 15 to 23°C and a humidity of 60 to 65% to be cultured in the dark. (6) Cultivation in light: The controlled conditions for culture room a are: 18-22℃, 600-800 lx, humidity 65%-85%, and continuous incubation in light for 18-40 hours. b. After the mycelia on the culture medium turn yellow, use sterile water to remove the mycelia from the surface of the culture medium. (7) Grass aging: First, use a disposable medical syringe needle to make 3 to 5 holes on the sealed plastic film as ventilation holes. The control conditions of the cultivation room during the grass aging stage are: light time 14 to 16 hours, cultivation temperature 20 to 24℃, and humidity 65% ​​to 85%. Nighttime darkness for 6–10 hours, culture temperature 15–17℃, temperature difference between light and dark environments 7–9℃, light source: white light (800 ppm) 1000 lx; ​​2–4 days; (8) When the Cordyceps militaris fruiting bodies grow to 0.5–1 cm in height, adjust the light intensity to 200–400 lx and the culture temperature to 18– 22℃, humidity 65%~85%, 12~14 hours of sunlight per day; (9) Harvesting: When the fruiting bodies of Cordyceps militaris grow to 5-8cm, the whole Cordyceps militaris plant can be harvested. After harvesting, it is dried for 1-2 days, and then dried at a temperature of 45-60℃ until the moisture content of the whole Cordyceps militaris plant is below 8%. Then it is stored in a refrigerator and frozen.

2. The cultivation method of the bee pupa armillaria of claim 1, characterized by: In step (1), frozen honeybee pupae of various ages from the mature larval stage to the metamorphosis stage are selected.

3. The cultivation method of the bee-horned Cordyceps as claimed in claim 1, characterized by: In step (2), select recently preserved Cordyceps militaris test tubes and expose them to light 48 hours before inoculation, and select those with better color change for later use.

4. The cultivation method of the bee-horned Cordyceps as claimed in claim 1, characterized by: In step (3)a, select pure natural mature honey; in step (3)b, put the formula in step (3)a into 1000ml of purified water, mix well, add 2 egg whites and stir thoroughly, fill into 2000ml glass measuring cups, sterilize at 0.137MPa and 121℃ for 30-40min, and then let stand for 10-12 hours; in step (3)c, filter and purify the solution after standing with sixteen layers of sterile gauze, and then fill into 5 500ml Erlenmeyer flasks, continue to sterilize at 0.137MPa and 121℃ for 30-40min, and when cooled to 95-85℃, quickly take it out and send it to the inoculation room; In step (3)d, the selected Cordyceps militaris test tube culture is exposed to light for 24-48 hours to change color. The outer wall of the test tube is then wiped with 75% alcohol for disinfection. In a sterile room, 3-5 pieces of 0.2×0.2mm mycelial blocks are picked from the test tube using a transplanting spatula and inoculated into step (3)c. The culture is then placed at 18-24℃ in the dark for 36 hours. After that, the culture is carried out in a shaker at 17-24℃ and 155r / min in the dark for 5-7 days. When the liquid culture medium is covered with mycelium, the culture is removed and placed in a refrigerator at 4℃ for later use.

5. The method for cultivating Cordyceps militaris according to claim 1, characterized in that: In step (4), bee pupae are used as raw materials and packaged into culture bottles with a height of 10cm and a diameter of 7cm. Each bottle contains 30g and is sealed with a high-temperature resistant polypropylene film. The bottles are sterilized under normal pressure for 240-360 minutes or under high pressure for 35-45 minutes. After cooling, they are placed in a sterile room for later use.

6. The method for cultivating Cordyceps militaris according to claim 1, characterized in that: In step (5), the prepared liquid bacterial culture is injected into the culture bottle using a pipette, with 1.5 to 4 mL inoculated into each bottle. The liquid bacterial culture is sprayed into each culture bottle 1 to 2 times. After sealing, the bottle is placed in a culture room with a temperature of 15 to 23°C and a humidity of 60 to 65% and cultured in the dark for 12 to 15 days.

7. The cultivation method of the bee-horned Cordyceps as claimed in claim 1, wherein: In step (6)a, the controlled conditions of the culture room are: 18-22℃, white light 600-800 lx, humidity 65%-85%, and continuous light culture for 18-40 hours until yellow; in step (6)b, after the mycelium of the culture medium turns yellow, the mycelium on the surface of the culture medium is removed with sterile water, and then the culture is sealed and continued.

8. The cultivation method of the bee-horned Cordyceps as claimed in claim 1, characterized by: In step (7), 3 to 5 holes are first punched in the sealed plastic film using a disposable medical syringe needle as ventilation holes. The control conditions of the cultivation room during the grass-inducing stage are: light time 14 to 16 hours, cultivation temperature 20 to 24°C, humidity 65% ​​to 85%; dark light at night 6 to 10 hours, cultivation temperature 15 to 17°C, the temperature difference between light and dark is 7 to 9°C, and appropriate ventilation is provided every day to ensure fresh air. The light is white light: 800 to 1000 lx; ​​2 to 4 days.

9. The cultivation method of the bee-horned Cordyceps as claimed in claim 1, wherein: In step (8), when the fruiting body of Cordyceps militaris grows to 0.5-1cm in height, the light intensity is adjusted to 200-400lx, the culture temperature is 18-22℃, the humidity is 65%-85%, the fruiting body is exposed to light for 12-14 hours per day, and the culture time is 30-45 days.

10. The cultivation method of the bee-horned Cordyceps as claimed in claim 1, wherein: In step (9), the dried bee pupa cordyceps is cooled, sorted, packaged and stored. When sorting, it is graded and packaged according to shape, size and quality. The storage conditions are: store away from light and at a temperature below -5℃.