A detection electrode and electrochemical sensor

Abstract

This application relates to a detection electrode and an electrochemical sensor. The detection electrode includes a detection substrate, multiple detection cells and detection chambers evenly distributed on the sidewalls of the detection substrate, a connecting patch disposed within the detection chamber, a detection electrode assembly with its first end electrically connected to the connecting patch and its second end extending into the detection cell, a protective shell slidably connected to the detection substrate and configured to enclose the detection cells and detection chamber, a flow guide plate slidably connected to the detection substrate and capable of extending into the detection cell, and a flow guide sponge disposed on the flow guide plate. When the protective shell slides, it pushes the detection electrode assembly into contact with the flow guide sponge. The electrochemical sensor includes the above-described detection electrode. The detection electrode and electrochemical sensor disclosed in this application can provide multiple detections in a single operation, with multiple detections performed simultaneously. Multiple detection results can be obtained based on a single blood sample. The electrode is also less susceptible to background interference during the detection process, resulting in more accurate detection results.

Description

Technical Field

[0001] This application relates to the field of detection technology, and in particular to a detection electrode and an electrochemical sensor. Background Technology

[0002] Electrochemical sensors are widely used in component detection, determining the change in electrical impedance between electrodes by applying a voltage of known value and form across them. Different coatings on the electrodes sensitize different substances, thus allowing for the detection of specific substances.

[0003] For example, with the electrode charged, the concentration of the analyte is measured by the current generated by the oxidation or reduction reaction of the analyte. Typically, a negatively charged electrode donates electrons to the analyte in a reduction reaction where the analyte becomes more negatively charged, while a positively charged electrode accepts electrons from the analyte in an oxidation (or ionization) reaction where the analyte becomes more positively charged. The charged electrode (or working electrode) induces a reaction and accepts or donates electrons to generate a current that provides the output signal; the specific content of the analyte can be determined by detecting the current value.

[0004] There are many items in blood testing, and electrochemical detection has more advantages. For example, a blood sample can be used for more than a dozen or even dozens of tests at the same time. However, it requires repeating the same or similar steps, and the current electrode printing method makes the electrodes susceptible to background interference. Summary of the Invention

[0005] This application provides a detection electrode and an electrochemical sensor that can provide multiple detections in a single operation, with the multiple detections performed simultaneously. Multiple detection results can be obtained based on a single blood sample. The electrode is also less susceptible to background interference during the detection process, resulting in more accurate detection results.

[0006] The above-mentioned objective of this application is achieved through the following technical solution:

[0007] In a first aspect, this application provides a detection electrode, comprising:

[0008] Detection matrix;

[0009] Multiple detection pools and detection chambers are evenly distributed on the sidewalls of the detection substrate;

[0010] The connection patch is located inside the testing chamber;

[0011] The detection electrode assembly has one end electrically connected to the connecting patch, and the other end extending into the detection cell;

[0012] The protective shell is slidably connected to the detection substrate and is configured to enclose the detection pool and detection chamber, and the dynamic detection electrode group is in contact with the flow guiding sponge.

[0013] The guide plate is slidably connected to the detection substrate and can extend into the detection pool; and

[0014] The flow-guiding sponge is placed on the flow-guiding plate.

[0015] In one possible implementation of the first aspect, a detection circuit disposed on the detection substrate is further included, the detection circuit being electrically connected to each connection patch.

[0016] In one possible implementation of the first aspect, the detection electrode assembly includes a connecting plate and multiple electrodes disposed on the connecting plate;

[0017] Both ends of the electrode extend from the connecting plate;

[0018] The detection substrate is provided with a slot that matches the connecting plate. The slot is located between a set of corresponding detection pools and detection chambers.

[0019] In one possible implementation of the first aspect, the connection between the electrode and the connecting patch is a detachable connection.

[0020] In one possible implementation of the first aspect, a guide ring is also included, which is sequentially connected to each guide plate;

[0021] The flow-guiding sponge extends onto the flow-guiding ring.

[0022] In one possible implementation of the first aspect, each flow-guiding sponge is coated with a different reagent.

[0023] In one possible implementation of the first aspect, the guide ring has flanks extending in the circumferential direction, and the protective shell can abut against the flanks to enclose the detection pool.

[0024] In one possible implementation of the first aspect, one end of the flow-guiding sponge passes through a notch on the side wing.

[0025] In a second aspect, this application provides an electrochemical sensor, including a detection electrode as described in the first aspect and any implementation thereof.

[0026] Overall, the detection electrode and electrochemical sensor disclosed in this application can provide multiple detections in a single operation, with these detections occurring simultaneously, enabling multiple test results to be obtained from a single blood sample. Furthermore, since the detection electrode assembly is not manufactured using printing methods, problems such as printing adhesion, contact area, and electrodes being on the same plane are avoided. This makes the detection electrode assembly less susceptible to environmental influences and less affected by background noise during the detection process, resulting in more accurate test results. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the internal structure of a detection electrode provided in this application.

[0028] Figure 2 This is a schematic diagram of the distribution of a detection pool on a detection substrate provided in this application.

[0029] Figure 3 This is a schematic diagram of the distribution of a detection chamber on a detection substrate provided in this application.

[0030] Figure 4 This is a schematic diagram of the current direction on an electrode provided in this application.

[0031] Figure 5 This is a schematic diagram of the initial state of a detection electrode provided in this application during use.

[0032] Figure 6 This is a schematic diagram of a flow guide ring as it unfolds, as provided in this application.

[0033] Figure 7 This is a schematic diagram of the structure of a protective shell provided in this application.

[0034] Figure 8 This is a schematic diagram of a protective shell provided in this application in its initial position.

[0035] Figure 9 This is a schematic diagram of a protective shell provided in this application when it is located at the pushing electrode position.

[0036] Figure 10 This is a schematic diagram of a planar structure for detecting optical discs provided in this application.

[0037] Figure 11 This is a schematic block diagram of a detection circuit provided in this application.

[0038] Figure 12 This is a schematic diagram of the structure of a detection electrode assembly provided in this application.

[0039] In the diagram, 3 is the protective shell, 31 is the first protective shell, 32 is the second protective shell, 33 is the guide block, 5 is the detection circuit, 11 is the detection substrate, 12 is the detection pool, 13 is the detection chamber, 21 is the connecting patch, 22 is the detection electrode group, 41 is the flow guide plate, 42 is the flow guide sponge, 43 is the flow guide ring, 44 is the side wing, 111 is the slot, 221 is the connecting plate, and 222 is the electrode. Detailed Implementation

[0040] The technical solutions in this application will be further described in detail below with reference to the accompanying drawings.

[0041] Please see Figure 1This application discloses a detection electrode, which consists of a detection substrate 11, a detection cell 12, a detection chamber 13, a connecting patch 21, a detection electrode group 22, a flow guide plate 41, and a flow guide sponge 42. The detection substrate 11 is cylindrical in shape. The detection cell 12 and the detection chamber 13 are both located on the side wall of the detection substrate 11. The number of the two is the same and they correspond one-to-one, that is, one detection cell 12 is matched with one detection chamber 13.

[0042] Both the detection pool 12 and the detection chamber 13 are evenly arranged on the side of the detection substrate 11 around its axis, such as... Figure 2 and Figure 3 As shown.

[0043] The connecting patch 21 is located inside the detection chamber 13, serving as a relay station between the detection electrode assembly 22 and the detection station. The detection station applies voltage to the detection electrode assembly 22 connected to the connecting patch 21 via the connecting patch 21, and then determines the content of a specific substance in the analyte by using the current signal fed back from the detection electrode assembly 22, such as... Figure 4 As shown.

[0044] As mentioned earlier, a negatively charged electrode provides electrons to the analyte in the reduction reaction in which the analyte becomes more negatively charged, while a positively charged electrode receives electrons from the analyte in the oxidation (or ionization) reaction in which the analyte becomes more positively charged.

[0045] The charged electrode (or working electrode) induces a reaction and receives or provides electrons to generate a current that provides an output signal. When different coatings are applied to the detection electrode assembly 22, different redox reactions can be induced during contact with the analyte. These reactions have different intensities and produce different current values. By detecting the current values, the content of a specific substance in the analyte can be inferred.

[0046] Please see Figure 1 The first end of the detection electrode assembly 22 is electrically connected to the connecting patch 21, and the second end extends into the detection cell 12. The guide plate 41 is slidably connected to the detection substrate 11 and can extend into the detection cell 12. The guide plate 41 is provided with a guide sponge 42. The function of the guide sponge 42 is to adsorb the analyte and then carry the analyte attached to the guide sponge 42 into the detection cell 12 along with the guide plate 41.

[0047] Please see Figure 5 and Figure 6 The initial state of the flow guide plate 41 is that one part is located outside the detection cell 12 and the other part is located inside the detection cell 12. The part of the flow guide plate 41 located outside the detection cell 12 first comes into contact with the analyte. This part of the analyte is transferred to the flow guide sponge 42 under the siphon effect and flows along the flow guide sponge 42 until all parts of the flow guide sponge 42 are filled with analyte.

[0048] Please see Figure 1 During this process, a thrust is applied to the guide plate 41, causing the guide plate 41 to slide into the detection pool 12. At this time, the protective shell 3, which is slidably connected to the detection substrate 11, is pushed, and the protective shell 3 begins to move towards the detection pool 12, covering part or all of the detection pool 12.

[0049] During this process, the protective shell 3 pushes the detection electrode assembly 22 into contact with the flow-guiding sponge 42, such as... Figure 7 As shown.

[0050] Please see Figure 7 The specific driving method is as follows: The protective shell 3 consists of two parts, namely the first protective shell 31 which is slidably connected to the detection substrate 11 and the second protective shell 32 which is rotatably connected to the first protective shell 31. The inner wall of the second protective shell 32 is provided with multiple guide blocks 33, and each guide block 33 is located in a detection pool 12.

[0051] Please see Figure 8 and Figure 9 After the first protective shell 31 and the second protective shell 32 stop moving, rotate the second protective shell 32. At this time, the guide block 33 will contact the detection electrode group 22 and push the detection electrode group 22 to contact the flow guiding sponge 42.

[0052] After the detection electrode assembly 22 comes into contact with the flow-guiding sponge 42, the detection station is activated and begins to apply voltage to the detection electrode assembly 22. At this time, the negatively charged electrodes donate electrons to the analyte in the reduction reaction where the analyte becomes more negatively charged, while the positively charged electrodes receive electrons from the analyte in the oxidation (or ionization) reaction where the analyte becomes more positively charged. The redox reaction generates a current at the detection electrode assembly 22, which is transmitted to the monitoring station through the connecting patch 21. The monitoring station analyzes the current to determine the content of a certain substance in the analyte.

[0053] Because each detection electrode assembly 22 within the detection cell 12 is coated with a different coating, different types of detection can be performed separately, meaning multiple detection processes can be completed in a single operation. The analytes used in this single operation can be prepared as follows:

[0054] Blood is dripped into the storage compartment of the test disc, which is then placed on a centrifuge. The centrifuge begins to rotate at its initial speed. The blood in the storage compartment flows into the separation chamber under centrifugal force, where it is separated into plasma and red blood cells. Figure 10 As shown.

[0055] The plasma or red blood cell fluid in the separation chamber flows into the finished product chamber for storage. A pharmaceutical chamber can be set up in the finished product chamber. Under the action of centrifugal force, the wall of the pharmaceutical chamber is broken, and the pharmaceutical fluid in the pharmaceutical chamber flows into the finished product chamber and mixes with the plasma or red blood cell fluid in the finished product chamber.

[0056] When using the detection electrodes provided in this application to detect the mixture in the finished product compartment of the detection disc, the guide plate 41 is aligned sequentially with each finished product compartment, and then the guide plate 41 is pressed into the finished product compartment. After the mixture in the finished product compartment has soaked the guide sponge 42 on the guide plate 41, the protective shell 3 is pushed until the protective shell 3 seals the detection pool 12. At this time, the detection electrode group 22 in each detection pool 12 will come into contact with the guide sponge 42.

[0057] This method can provide multiple tests in a single operation, with the tests performed simultaneously, allowing for multiple test results based on a single blood sample.

[0058] The detection electrode assembly 22 is suspended within the detection pool 12 and only comes into contact with the flow-guiding sponge 42 on the guide plate 41 during detection. After detection, it detaches from the flow-guiding sponge 42 on the guide plate 41. Because the detection electrode assembly 22 is not manufactured using printing methods, problems such as printing adhesion, contact area, and electrodes being on the same plane are avoided. This makes the detection electrode assembly 22 less susceptible to environmental influences and less affected by background noise during the detection process, resulting in more accurate detection results.

[0059] Please see Figure 11 As a specific embodiment of the detection electrode provided in this application, a detection circuit 5 connected to the connecting patch 21 is added. The detection circuit 5 is located on the detection substrate 11 and its function is to directly convert the current signal generated by the detection electrode group 22 into a corresponding detection signal output. The purpose of this method is to further improve the integration of this application, so that the detection electrode disclosed in this application can be directly used for detection, and the detected value can be directly transmitted to a computer for display via a data cable.

[0060] In some possible implementations, the detection circuit 5 consists of a microcontroller and two branch circuits. The first branch circuit connects the microcontroller and the auxiliary electrode to the reference electrode. This branch circuit consists of a voltage scanning circuit and a constant potential circuit connected to the microcontroller. The constant potential circuit is connected to the auxiliary electrode and the reference electrode.

[0061] The second branch circuit consists of a current / voltage conversion circuit, a low-pass filter, a voltage amplifier circuit, and an A / D conversion circuit connected in sequence. The output of the A / D conversion circuit is connected to the microcontroller, and the current / voltage conversion circuit is connected to the working electrode.

[0062] Please see Figure 12 As a specific implementation of the detection electrode provided in the application, the detection electrode group 22 includes a connecting plate 221 and multiple electrodes 222 disposed on the connecting plate 221, with both ends of the electrodes 222 extending out from the connecting plate 221.

[0063] Please see Figure 8 The detection substrate 11 is provided with a slot 111 that matches the connecting plate 221. The slot 111 is located between a set of corresponding detection pools 12 and detection chambers 13. The function of the slot 111 is to fix the connecting plate 221. When the connecting plate 221 is inserted into the slot 111, one end of the electrode 222 extends into the detection pool 12, and the other end extends into the detection chamber 13 corresponding to the detection pool 12.

[0064] This structure makes the detection electrode assembly 22 easy to replace. The purpose of replacement is twofold: first, to replace it in time when it is damaged; and second, to replace the corresponding detection electrode assembly 22 when the detection requirements are different.

[0065] The connection between electrode 222 and connecting patch 21 is a detachable connection, such as a bolt connection or a pressure connection, both of which can achieve quick assembly and disassembly.

[0066] Please see Figure 6 As a specific implementation of the detection electrode provided in the application, a flow guide ring 43 is added that is sequentially connected to each flow guide plate 41, and the flow guide sponge 42 extends to the flow guide ring 43. However, it should be noted that adjacent flow guide sponges 42 do not contact each other.

[0067] The function of the flow guide ring 43 is to drive multiple flow guide plates 41 to move synchronously. When the flow guide ring 43 is pressed, all the flow guide plates 41 on the detection base 11 will move synchronously.

[0068] Furthermore, the guide ring 43 has an advantage when replacing the guide plate 41, because all the guide plates 41 on the detection substrate 11 can be directly removed by pulling the guide ring 43.

[0069] In some possible implementations, each flow-guiding sponge 42 is coated with a different reagent, which serves the same purpose as the reagent in the reagent chamber mentioned above. The reagent coated on the flow-guiding sponge 42 replaces the reagent in the reagent chamber of the detection disc. When the flow-guiding sponge 42 is coated with reagent, the detection disc mentioned above can be used for blood separation.

[0070] Please see Figure 6As a specific embodiment of the detection electrode provided in the application, the flow guide ring 43 has side wings 44 extending in the circumferential direction. The function of the side wings 44 is to cooperate with the protective shell 3 to seal the detection cell 12. After the detection cell 12 is sealed, it can prevent external air from entering the detection cell 12, which can effectively prevent the analytes on the flow guide sponge 42 from being oxidized.

[0071] When in use, push the protective shell 3, and the protective shell 3 can directly abut against the side wing 44.

[0072] After the addition of side wings 44 to the flow guide ring 43, there are notches on the side wings 44. One end of the flow guide sponge 42 passes through the notch on the side wings 44 and can directly suck up the analyte.

[0073] This application also discloses an electrochemical sensor, including any of the detection electrodes mentioned above.

[0074] The embodiments described in this specific implementation are preferred embodiments of this application and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A detection electrode, characterized by, The utility model relates to a detection electrode, including: a detection base (11); a plurality of detection pools (12) and detection chambers (13) are evenly distributed on the side wall of the detection base (11); a connecting patch (21) is arranged in the detection chamber (13); a detection electrode group (22) is electrically connected to the first end of the connecting patch (21) and extends into the detection pool (12); a protective shell (3) is slidably connected to the detection base (11) and is configured to close the detection pool (12) and the detection chamber (13) and push the detection electrode group (22) into contact with a flow guide sponge (42); a flow guide plate (41) is slidably connected to the detection base (11) and can extend into the detection pool (12); and the flow guide sponge (42) is arranged on the flow guide plate (41); a flow guide ring (43) is sequentially connected to each flow guide plate (41); the flow guide sponge (42) extends to the flow guide ring (43); each flow guide sponge (42) is coated with a different reagent; a wing (44) extends in the circumferential direction of the flow guide ring (43); the protective shell (3) can abut against the wing (44) to close the detection pool (12); one end of the flow guide sponge (42) passes through a gap in the wing (44); the protective shell (3) is composed of two parts, namely a first protective shell (31) that is slidably connected to the detection base (11) and a second protective shell (32) that is rotatably connected to the first protective shell (31); a plurality of guide blocks (33) are arranged on the inner wall of the second protective shell (32), and each guide block (33) is located in a detection pool (12).

2. The detection electrode according to claim 1, characterized in that a detection circuit (5) is arranged on the detection base (11) and is electrically connected to each connecting patch (21).

3. The detection electrode according to claim 1, characterized by The detection electrode group (22) includes a connecting card (221) and a plurality of electrodes (222) arranged on the connecting card (221); both ends of the electrode (222) extend from the connecting card (221); a card slot (111) that matches the connecting card (221) is arranged on the detection base (11) and is located between a corresponding group of detection pools (12) and detection chambers (13).

4. The detection electrode according to claim 3, characterized in that The electrode (222) is detachably connected to the connecting patch (21).

5. An electrochemical sensor, characterized in that The utility model relates to a detection electrode, including: as claimed in any one of claims 1 to 4.

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