A composition with hair growth and hair strengthening functions, preparation method and application

Abstract

The present invention discloses a composition with hair growth and hair strengthening functions, a preparation method and an application, belonging to the technical field of hair growth and hair strengthening. The raw materials of the composition include, by weight: main components, including 1020 - 3050 parts of protein and 22.4 - 140 parts of nutritional fortifier; efficacy components, including 20 - 200 parts of powdered plant sterol ester, 100 - 500 parts of millet extract, 100 - 500 parts of polygonatum sibiricum powder, 100 - 400 parts of mulberry powder and 100 - 500 parts of aronia melanocarpa fruit powder; auxiliary components, including 200 - 1800 parts of flavoring agent. The composition of the present invention has no toxic and side effects, can promote the metabolism and proliferation of hair follicle cells, can effectively promote the formation of melanin in hair follicles, shorten the time for hair follicles to enter the growth phase from the resting phase and grow hair, and can strengthen the tightness of hair on the scalp, thereby achieving the effect of preventing hair loss and strengthening hair.

Description

Technical Field

[0001] The present invention belongs to the technical field of hair growth and hair strengthening, and particularly relates to a composition with hair growth and hair strengthening functions, a preparation method and an application thereof. Background Art

[0002] Statistics show that hair loss has become more and more prevalent and younger.

[0003] There are many products on the market that claim to have hair growth and hair strengthening functions. However, the hair growth and hair strengthening effects of these products are not very satisfactory. Moreover, most hair growth products are hair washing and care products, and there are few other products. However, since hair washing and care products need to have a cleaning function, they will inevitably add chemical components such as surfactants and conditioners that can damage the hair. Therefore, there is an urgent need for a composition with good hair growth and hair strengthening functions. Summary of the Invention

[0004] To solve the above problems in the prior art, the present invention provides a composition with hair growth and hair strengthening functions, a preparation method and an application thereof.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] One of the technical solutions of the present invention: A composition with hair growth and hair strengthening functions, the raw materials of the composition with hair growth and hair strengthening functions include, by weight:

[0007]

[0008] Further, the protein includes small molecule peptides and amino acids; the small molecule peptides include 500 - 1500 parts of bovine bone collagen peptides and 500 - 1500 parts of hydrolyzed collagen peptides by weight; the amino acids include 20 - 50 parts of taurine by weight.

[0009] Further, the nutritional fortifier is a vitamin fortifier and a mineral fortifier.

[0010] Still further, the vitamin fortifier includes 0.2 - 10 parts of vitamin A, 10 - 50 parts of vitamin C, 1 - 10 parts of vitamin E and 10 - 50 parts of yeast powder by weight; the mineral fortifier includes 1 - 10 parts of ferrous fumarate and 0.2 - 10 parts of zinc oxide by weight.

[0011] Further, the powdered phytosterol ester is β-sitosterol ester, and the chemical formula is C 47 O2H 80 .

[0012] Further, the preparation method of the millet extract is as follows: pulverize the millet, extract with water, concentrate the extract, and freeze-dry to obtain the millet extract.

[0013] Further, the flavoring agent includes 100 - 500 parts by weight of erythritol and 100 - 500 parts by weight of grapefruit powder.

[0014] The second technical solution of the present invention: The preparation method of the above-mentioned composition with hair growth and hair strengthening functions includes the following steps:

[0015] (1) Weigh each raw material according to parts by weight, and sieve for later use;

[0016] (2) Mix the nutritional fortifier and the flavoring agent weighed in step (1) to obtain the mixed raw material A;

[0017] (3) Place the protein and the active ingredient weighed in step (1) in a granulator and stir, use alcohol as a binder for granulation, then dry in a fluidized bed, and size with a sizing machine to obtain the mixed raw material B;

[0018] (4) Mix the mixed raw material A obtained in step (2) with the mixed raw material B obtained in step (3), and sieve to obtain the composition with hair growth and hair strengthening functions.

[0019] Further, in step (1), the sieving is through a 40 - 100 mesh sieve.

[0020] Further, in step (2), the mixing is an equal - increment mixing.

[0021] Further, in step (3), the stirring speed is 200 - 500 revolutions per minute; the amount of alcohol used is to spray 280 - 480 parts by mass of 70 - 95% alcohol within 2 - 10 minutes; the fluidized bed drying temperature is 51 - 58 °C, and the time is 25 - 35 minutes; the sizing machine is a 20 - mesh sizing machine.

[0022] Further, in step (4), the sieving is through a 40 - 100 mesh sieve.

[0023] The third technical solution of the present invention: The application of the above - mentioned composition with hair growth and hair strengthening functions in hair growth and hair strengthening.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] (1) The composition with hair growth and hair strengthening functions prepared by the present invention can shorten the time for hair follicles to enter the growth phase from the resting phase, can accelerate the time for hair to grow, and has a significant promoting effect on hair growth;

[0026] (2) The composition with hair growth and hair strengthening functions prepared by the present invention has no toxic or side effects and can effectively promote the growth of new hair follicles;

[0027] (3) The present invention uses fluidized bed granulation instead of oven drying. Compared with oven drying, its advantages are that the fluid state is uniform, the drying is uniform, the damage to the surface of the material is small, and the powder is more porous; the thermal efficiency is high, the energy-saving effect is good, and it can save 30-60% energy compared with oven drying.

[0028] The hydrolyzed collagen peptide added in the present invention is an essential component of human skin, which can effectively ensure the moisture of the skin, increase the firmness of the skin, and the main protein in hair is keratin. Collagen may support and increase the hair growth protein in the body. This can help strengthen the hair, promote hair growth, and prevent hair loss;

[0029] In addition, two types of collagen peptides are selected in this application because one is the collagen peptide extracted from animal skin and the other is the collagen peptide derived from bone. The amino acid components and peptide segments are different and can complement each other;

[0030] The taurine added in the present invention not only has the function of repairing damaged hair, but also has the function of increasing the generation of hair growth factors and promoting hair growth. Taurine can increase the production of growth factor IGF-1, which can act on the hair cycle of humans, extend the growth phase, and produce thick and strong hair;

[0031] The various vitamins added in the present invention can increase the nutrition of the scalp, promote the metabolism of hair follicle cells, and thus promote hair growth;

[0032] The yeast powder added in the present invention contains vitamin H and niacin. The vitamin H contained in it, also known as biotin, is a necessary nutrient for maintaining the natural growth, development and normal body functions of the human body. It can directly regulate the metabolism of hair follicle cells and can also indirectly affect hair follicle development by affecting food intake or metabolism;

[0033] In addition, the niacin contained in the yeast powder is also an essential nutrient in the human body and plays an important role in promoting the normal growth and development of the human body. It can promote iron absorption and the formation of blood cells and maintain the normal functions of the skin;

[0034] The present invention adds ferrous fumarate. Iron provides oxygen for every cell in the body, including the cells in the scalp and hair follicles;

[0035] The present invention adds zinc oxide. Zinc is a cofactor of various enzymes in the body and is also an important component of antioxidant enzymes in the body. It can strengthen the activity of blood cells and is an essential trace element for hair growth and keratin synthesis;

[0036] The powdered plant sterol ester added in the present invention is prepared by esterification reaction or transesterification reaction of plant sterol and fatty acid. Since sterol ester can be converted into sterol and fatty acid in the human body, its physiological functions include those of both plant sterol and fatty acid. Plant sterol ester is more easily absorbed by the human body, and its utilization efficiency is much higher than that of ordinary plant sterol. It can prevent hair loss by inhibiting the production of androgen;

[0037] The millet extract added in the present invention is a rare oil extracted from panicum miliaceum, specifically panicolin (chemical formula: C 31 H 52 O). This rare oil acts on the root of hair follicles, can accelerate cell metabolism and proliferation, keep hair growing, nourish and repair damaged scalp, create good basic conditions for healthy hair quality, and also has anti-inflammatory and anti-toxic effects;

[0038] The polygonatum sibiricum powder added in the present invention belongs to the kidney meridian and has the effect of tonifying essence and marrow, and has a good effect on treating alopecia of kidney deficiency type, etc.;

[0039] The mulberry powder added in the present invention has the effects of tonifying the kidney and benefiting essence, blackening hair and promoting hair growth, and is applicable to symptoms such as premature aging, early whitening of hair, hair loss, alopecia areata, and withered hair; Mulberry is a material in the catalog of homologous medicines and foods. According to the pharmacopoeia, it has the effect of treating early whitening of hair; Mulberry is rich in proanthocyanidins. Proanthocyanidins may promote hair regeneration by activating stem cells in the bulge of hair follicles and inducing the transformation of resting hair follicles into growth phase;

[0040] The fruit powder of aronia melanocarpa added in the present invention is rich in various vitamins, antioxidant components and mineral elements, and has the effect of improving blood circulation in the head. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0042] Figure 1 It is the diagram of the hair change on the back of the mouse for the 3# dosing sample;

[0043] Figure 2 It is the diagram of the hair change on the back of the mouse for the low-dose dosing sample;

[0044] Figure 3 It is the diagram of the hair change on the back of the mouse for the high-dose dosing sample;

[0045] Figure 4The figure shows the hair changes on the back of the mice administered with the positive control sample for drug administration;

[0046] Figure 5 The figure shows the hair changes on the back of the mice administered with the negative control sample for drug administration;

[0047] Figure 6 The figure is a statistical chart of the number of newly generated hair follicles under the microscope for the mice administered with sample 3#, low-dose sample, high-dose sample, positive control sample, and negative control sample;

[0048] Figure 7 The figure is the HE staining map of the mice administered with sample 3#, where (a) is the HE staining map of the skin tissue of the mice at 7 days, (b) is the HE staining map of the skin tissue of the mice at 14 days, (c) is the HE staining map of the skin tissue of the mice at 28 days, and (d) is the HE staining map of the skin tissue of the mice at 56 days;

[0049] Figure 8 The figure is the HE staining map of the mice administered with the low-dose sample, where (a) is the HE staining map of the skin tissue of the mice at 7 days, (b) is the HE staining map of the skin tissue of the mice at 14 days, (c) is the HE staining map of the skin tissue of the mice at 28 days, and (d) is the HE staining map of the skin tissue of the mice at 56 days;

[0050] Figure 9 The figure is the HE staining map of the mice administered with the high-dose sample, where (a) is the HE staining map of the skin tissue of the mice at 7 days, (b) is the HE staining map of the skin tissue of the mice at 14 days, (c) is the HE staining map of the skin tissue of the mice at 28 days, and (d) is the HE staining map of the skin tissue of the mice at 56 days;

[0051] Figure 10 The figure is the HE staining map of the mice administered with the positive control sample, where (a) is the HE staining map of the skin tissue of the mice at 7 days, (b) is the HE staining map of the skin tissue of the mice at 14 days, (c) is the HE staining map of the skin tissue of the mice at 28 days, and (d) is the HE staining map of the skin tissue of the mice at 56 days;

[0052] Figure 11 The figure is the HE staining map of the mice administered with the negative control sample, where (a) is the HE staining map of the skin tissue of the mice at 7 days, (b) is the HE staining map of the skin tissue of the mice at 14 days, (c) is the HE staining map of the skin tissue of the mice at 28 days, and (d) is the HE staining map of the skin tissue of the mice at 56 days. Detailed implementation mode

[0053] Now, various exemplary implementation modes of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention. It should be understood that the terms described in the present invention are only for describing specific implementation modes and are not used to limit the present invention.

[0054] In addition, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0055] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0056] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific embodiments of the description of the present invention, which are obvious to those skilled in the art. Other embodiments obtained from the description of the present invention are obvious to those skilled in the art. The description and examples of the present invention are merely exemplary.

[0057] Regarding the use of "comprising", "including", "having", "containing", etc. herein, they are all open-ended terms, meaning including but not limited to.

[0058] In the embodiments of the present invention, unless otherwise specified, the "parts" mentioned are all "parts by weight".

[0059] In the following examples, the powdered plant sterol ester is prepared according to Patent CN201410445798.0;

[0060] In the following examples, the preparation method of the millet extract is as follows:

[0061] 1) Place the millet in a hot air drying oven at 37 °C and dry for 24 h, then pulverize and pass through an 80-mesh sieve;

[0062] 2) Mix the sieved millet obtained in step 1) with water at a solid-liquid ratio of 1:10, vortex extract at 50 °C for 1 h, filter by suction, repeat twice, and collect the filtrate and filter residue respectively;

[0063] 3) Concentrate the filtrate obtained in step 2) to a solid content of 25-35% to obtain a concentrated solution;

[0064] 4) Freeze-dry the concentrated solution obtained in step 3) to obtain the millet extract, wherein the parameters of freeze-drying are to keep the moisture below 6%;

[0065] In the following examples, the mulberry powder, aronia melanocarpa fruit powder, and polygonatum sibiricum powder are commercially available. Among them, the proanthocyanidin content in the mulberry powder and aronia melanocarpa fruit powder is 10%, and the polygonatum polysaccharide content in the polygonatum sibiricum powder is 15%.

[0066] In the following examples, the bovine bone collagen peptide and hydrolyzed collagen peptide are commercially available and are both food raw materials with a protein content of 95%.

[0067] In the following examples, the vitamin A is commercially available, is a food-grade additive, and the content of the active substance is 325,000 IU / g.

[0068] In the following examples, the vitamin C is commercially available, is a food-grade additive, and the content of the active substance is 97%.

[0069] In the following examples, the taurine and ferrous fumarate are commercially available and are both food-grade additives with an active substance content of 99%.

[0070] In the following examples, the vitamin E is commercially available, is a food-grade additive, and the content of the active ingredient is 50%.

[0071] In the following examples, the yeast powder is commercially available, and the content of the active substance niacin is 40 mg / g and the biotin content is 300 ppm.

[0072] In the following examples, the term "equal increment mixing" means: equal increment means taking the raw material with the least amount of use and an equal amount of other raw materials, mixing them evenly, and then adding an equal amount of other raw materials as the mixture and mixing them evenly. In this way, the amount is increased by multiples until all the raw materials are mixed.

[0073] Example 1

[0074] (1) Weigh each raw material according to the weight parts recorded in 1# in Table 1, and sieve through a 40-mesh sieve for standby.

[0075] (2) Mix the nutritional fortifiers and flavoring agents obtained in step (1) by equal increment to obtain the mixed raw material A.

[0076] (3) Place the protein and functional components obtained in step (1) in a granulator and stir. Use alcohol as a binder for granulation, then dry in a fluidized bed, and size with a 20-mesh sizing machine to obtain the mixed raw material B. Among them, the stirring speed is 200 revolutions / min, the amount of alcohol used is 280 parts of 70% alcohol sprayed within 2 minutes, the fluidized bed drying temperature is 51 °C, and the time is 25 minutes.

[0077] (4) Mix the mixed raw material A obtained in step (2) with the mixed raw material B obtained in step (3), and sieve through a 40-mesh sieve to obtain a composition with the function of promoting hair growth and preventing hair loss.

[0078] Example 2

[0079] (1) Weigh each raw material according to the parts by weight recorded in No. 2 of Table 1, and sieve through a 100-mesh sieve for standby;

[0080] (2) Gradually and equally increase the amount of the nutritional fortifier and flavoring agent weighed in step (1) and mix them to obtain mixed raw material A;

[0081] (3) Place the protein and functional components weighed in step (1) in a granulator and stir. Use alcohol as a binder to granulate, then dry in a fluidized bed, and size with a 20-mesh sizing machine to obtain mixed raw material B, where the stirring speed is 500 revolutions per minute, the alcohol dosage is to spray 480 parts of 95% alcohol within 10 minutes, the fluidized bed drying temperature is 58 °C, and the time is 35 minutes;

[0082] (4) Mix the mixed raw material A obtained in step (2) with the mixed raw material B obtained in step (3), and sieve through a 100-mesh sieve to obtain a composition with the functions of promoting hair growth and strengthening hair.

[0083] Example 3

[0084] (1) Weigh each raw material according to the parts by weight recorded in No. 3 of Table 1, and sieve through a 60-mesh sieve for standby;

[0085] (2) Gradually and equally increase the amount of the nutritional fortifier and flavoring agent weighed in step (1) and mix them to obtain mixed raw material A;

[0086] (3) Place the protein and functional components weighed in step (1) in a granulator and stir. Use alcohol as a binder to granulate, then dry in a fluidized bed, and size with a 20-mesh sizing machine to obtain mixed raw material B, where the stirring speed is 350 revolutions per minute, the alcohol dosage is to spray 300 parts of 85% alcohol within 5.5 minutes, the fluidized bed drying temperature is 55 °C, and the time is 30 minutes;

[0087] (4) Mix the mixed raw material A obtained in step (2) with the mixed raw material B obtained in step (3), and sieve through a 60-mesh sieve to obtain a composition with the functions of promoting hair growth and strengthening hair.

[0088] Table 1 Dosage of each raw material

[0089]

[0090] Comparative Example 1

[0091] Same as Example 3, the difference is that in step (1), each raw material is weighed according to the parts by weight recorded in No. 4 of Table 2, and sieve through a 60-mesh sieve for standby.

[0092] Comparative Example 2

[0093] Same as Example 3, except that in step (1), each raw material is weighed according to the parts by weight recorded in No. 5 of Table 2, and is reserved after passing through a 60-mesh sieve.

[0094] Comparative Example 3

[0095] Same as Example 3, except that in step (1), each raw material is weighed according to the parts by weight recorded in No. 6 of Table 2, and is reserved after passing through a 60-mesh sieve.

[0096] Comparative Example 4

[0097] Same as Example 3, except that in step (1), each raw material is weighed according to the parts by weight recorded in No. 7 of Table 2, and is reserved after passing through a 60-mesh sieve.

[0098] Comparative Example 5

[0099] Same as Example 3, except that in step (1), each raw material is weighed according to the parts by weight recorded in No. 8 of Table 2, and is reserved after passing through a 60-mesh sieve.

[0100] Comparative Example 6

[0101] Same as Example 3, except that in step (1), each raw material is weighed according to the parts by weight recorded in No. 9 of Table 2, and is reserved after passing through a 60-mesh sieve.

[0102] Comparative Example 7

[0103] Same as Example 3, except that in step (1), each raw material is weighed according to the parts by weight recorded in No. 10 of Table 2, and is reserved after passing through a 60-mesh sieve.

[0104] Comparative Example 8

[0105] Same as Example 3, except that in step (1), each raw material is weighed according to the parts by weight recorded in No. 11 of Table 2, and is reserved after passing through a 60-mesh sieve.

[0106] Comparative Example 9

[0107] Same as Example 3, except that in step (1), each raw material is weighed according to the parts by weight recorded in No. 12 of Table 2, and is reserved after passing through a 60-mesh sieve.

[0108] Comparative Example 10

[0109] Same as Example 3, except that in step (1), black bean powder is used to replace mulberry powder.

[0110] Dosage of Each Raw Material in Table 2

[0111]

[0112]

[0113] Effect Verification

[0114] I. Prescription Effect Verification

[0115] (I) Selection of Subjects

[0116] Qualified subjects are selected according to the inclusion and exclusion criteria;

[0117] 1. Inclusion Criteria

[0118] (1) Healthy males or females aged 25 to 45 years old;

[0119] The hair length is between 5 and 40 cm;

[0120] (2) Those who have problems with excessive hair loss and mild hair thinning, and the hair loss count is more than 10 roots according to the 60 - combing method and still more than 10 roots after a 2 - week preparation period;

[0121] (3) Those who have not had special hair beauty treatments such as hair dyeing, perming, and styling within the past month;

[0122] 2. Exclusion Criteria

[0123] (1) Pregnant or lactating women, or those with a recent pregnancy plan;

[0124] (2) Those suffering from mental or psychological diseases; or those with long - term sleep or emotional control disorders;

[0125] (3) Those who have used cosmetics with anti - hair - loss effects or other products with such effects or hair - growth effects within the past 3 months;

[0126] (4) Those who have taken or topically used any drugs that affect hair growth within the past 6 months;

[0127] 3. Restrictions on Subjects

[0128] (1) Subjects cannot wash their hair within 48 ± 4 hours before each visit during the screening and trial period, and the time of not washing hair before each visit should be basically the same. They cannot comb their hair by themselves on the day of the visit;

[0129] (2) Do not cut hair within 2 weeks before each visit assessment during the trial period;

[0130] (3) During the trial period, no hair care and hair beauty treatment measures can be carried out, and no anti - hair - loss or hair - growth treatments can be received;

[0131] (4) During the trial period, the original living habits should be maintained, and large mood swings should be avoided;

[0132] (II) Test Substances

[0133] Test samples: Compositions prepared from Examples 1 - 3 and Comparative Examples 1 - 9;

[0134] Usage: Randomly distribute the test samples to the subjects, and provide guidance on the use of the samples to ensure that the subjects correctly use the test substances during the test period. The test substances are used for 12 weeks. During the test, the subjects are required to record the usage time and any discomfort and adverse reaction symptoms during the usage process;

[0135] (III) Test Equipment

[0136] Comb: The density of the comb teeth is moderate (tooth spacing 0.9 - 1.1 mm), the length of the comb teeth is 2.0 - 3.0 cm, the length of the comb is not less than 10 cm (excluding the comb handle). The same specification and material of the comb must be used throughout the test process. After each use, disinfect it according to the relevant requirements in the "Technical Specification for Disinfection in Medical Institutions" (WS-T 367-2012);

[0137] (IV) Test Procedures

[0138] Recruit subjects as required. At the same time, use the 60 - combing method to count the number of hair loss and record it;

[0139] Qualified subjects undergo a 2 - week preparation period and use 0.9% sodium chloride solution. After the preparation period, perform the 60 - combing method again. Those with a hair loss count greater than 10 enter the formal test;

[0140] The subjects selected for the formal test are divided into 12 groups according to the stratified random method to ensure the balance of factors that may affect the test results, such as gender, age, hair length, severity of hair loss, etc.;

[0141] Evaluate the basic hair values of the selected subjects before using the product, including hair loss count, and record. Conduct the same evaluation and test again 4 weeks, 8 weeks, and 12 weeks after using the product;

[0142] (V) Hair Loss Count: At each visit of the subject, trained staff use the 60 - combing method to comb the subject's hair, count the fallen hair, and record.

[0143] (VI) Test Results

[0144] The verification results of the prescription effect are shown in Table 3;

[0145] Table 3 Verification Results of Prescription Effect

[0146]

[0147] (VII) Conclusion

[0148] It can be found according to the data in Table 3 that the difference in hair loss count after 12 weeks in Examples 1 to 3 (hair loss count before product use - hair loss count after 12 weeks of product use) is higher than that in Comparative Examples 1 to 10. Therefore, the anti - hair loss efficacy of Examples 1 to 3 is better than that of Comparative Examples 1 to 10. In addition, among Examples 1 to 3, the anti - hair loss effect of Example 3 is better than that of Example 1 and Example 2.

[0149] II. Dose - effect verification

[0150] (I) Experimental materials and methods

[0151] 1. Grouping of mice:

[0152] Fifty C57BL / 6J mice (SPF - level) aged 38 - 40 days and weighing 15 - 20 g (provided by the Experimental Animal Center of Southern Medical University) were randomly divided into 5 groups of 10 mice each after 3 days of adaptive feeding. The hair on the backs of the mice was shaved 24 hours before drug administration to induce the hair to enter the growth phase from the resting phase.

[0153] 2. Sample preparation:

[0154] Take 1.54 g of the composition prepared in Example 3 and completely dissolve it in 20 mL of water to obtain Sample 3#;

[0155] Take 0.77 g of the composition prepared in Example 3 and completely dissolve it in 20 mL of water to obtain a low - dose sample;

[0156] Take 3.08 g of the composition prepared in Example 3 and completely dissolve it in 20 mL of water to obtain a high - dose sample;

[0157] Prepare a 0.2% finasteride solution from finasteride solid to obtain a positive control sample;

[0158] Prepare a 0.9% sodium chloride solution from sodium chloride solid to obtain a negative control sample;

[0159] 3. Drug administration

[0160] Administer Sample 3# and the four groups of control samples to the 5 groups of mice respectively. The administration method is to intragastrically administer once a day, 0.2 mL each time, for 8 weeks.

[0161] (II) Experimental results

[0162] 1. Changes in mouse body weight

[0163] Weigh the mice every 7 days during the detection process and record the results, expressed as mean ± standard deviation. The specific results are shown in Table 4;

[0164] Table 4 Changes in mouse body weight (unit: g)

[0165]

[0166]

[0167] It can be found from the data in the above table that during the entire detection process, the weight change trends of the 5 groups of mice were basically the same, and there were no abnormalities in the mice.

[0168] 2. Time required for skin changes and hair growth in mice

[0169] Observe the skin and hair growth conditions at the hair removal sites of each group of mice every day. The specific hair growth conditions are as Figures 1 to 5 shown (photographed every 7 days), and record the time when the skin color of the hair removal area of each mouse changes from pink to black and the time when hair starts to grow, expressed as mean ± standard deviation. The specific results are shown in Table 5;

[0170] Figure 1 Figure showing the hair changes on the back of the mouse administered with test sample 3#;

[0171] Figure 2 Figure showing the hair changes on the back of the mouse administered with a low-dose test sample;

[0172] Figure 3 Figure showing the hair changes on the back of the mouse administered with a high-dose test sample;

[0173] Figure 4 Figure showing the hair changes on the back of the mouse administered with a positive control test sample;

[0174] Figure 5 Figure showing the hair changes on the back of the mouse administered with a negative control test sample;

[0175] As can be seen from the above figure, in test sample 3#, 2 mice had gray skin on D14, and 2 mice had grown hair. On D21, it was found that 4 mice had significantly grown hair, among which 1 mouse had 60% of the back area with hair growth, and 3 mice had full hair growth on the back;

[0176] In the low-dose test sample, 1 mouse had gray skin on D14, and on D21, it was found that 5 mice had grown hair, among which 2 mice had 70% of the back area with hair growth, and 2 mice had 80% of the back area with hair growth;

[0177] In the high-dose test sample, 5 mice had grown hair on the back on D14, and on D21, it was found that 5 mice had grown hair, among which 3 mice had 90% of the back area with hair growth, and 2 mice had full hair growth on the back;

[0178] In the positive control test sample, 4 mice had gray skin on D14, and one had significantly grown hair. On D21, it was found that 6 mice had 90% of the back area with hair growth.

[0179] In the negative control sample, 2 mice had gray skin on D14. On D21, it was found that 3 mice had hair growth on 70% of the back area, and 2 mice had hair growth on 90% of the back area.

[0180] Table 5 Time required for skin changes and hair growth in mice (unit: d)

[0181]

[0182]

[0183] From the data in the above table, it can be found that compared with the negative control sample, Sample 3#, the high-dose sample, and the positive control sample shortened the time to enter the growth phase and grow hair to varying degrees; among them, Sample 3# more significantly shortened the time for hair follicles to enter the growth phase from the resting phase and grow hair.

[0184] 3 Histological examination of mouse back skin

[0185] On the 7th day, 14th day, 28th day, and 56th day, after administration for 24 h, 2 mice were humanely sacrificed each time. Skin specimens were taken from the same part of the shaved area, fixed, routinely sectioned, stained with HE, and the number of newly formed hair follicles was expressed as the mean. The specific results are shown in Tables 6 - 9 and Figure 6 as follows;

[0186] Table 6 Histological indices of mouse skin on the 7th day (unit: number)

[0187]

[0188] Table 7 Histological indices of mouse skin on the 14th day (unit: number)

[0189]

[0190] Table 8 Histological indices of mouse skin on the 28th day (unit: number)

[0191]

[0192] Table 9 Histological indices of mouse skin on the 56th day (unit: number)

[0193]

[0194] The HE staining pictures of Sample 3#, low-dose sample, high-dose sample, positive control sample, and negative control sample are as Figures 7 to 11 follows:

[0195] Figure 7It is the HE staining diagram of specimen 3#, where (a) is the HE staining diagram of the skin histology of 7-day-old mice, (b) is the HE staining diagram of the skin histology of 14-day-old mice, (c) is the HE staining diagram of the skin histology of 28-day-old mice, and (d) is the HE staining diagram of the skin histology of 56-day-old mice;

[0196] Figure 8 It is the HE staining diagram of the low-dose specimen, where (a) is the HE staining diagram of the skin histology of 7-day-old mice, (b) is the HE staining diagram of the skin histology of 14-day-old mice, (c) is the HE staining diagram of the skin histology of 28-day-old mice, and (d) is the HE staining diagram of the skin histology of 56-day-old mice;

[0197] Figure 9 It is the HE staining diagram of the high-dose specimen, where (a) is the HE staining diagram of the skin histology of 7-day-old mice, (b) is the HE staining diagram of the skin histology of 14-day-old mice, (c) is the HE staining diagram of the skin histology of 28-day-old mice, and (d) is the HE staining diagram of the skin histology of 56-day-old mice;

[0198] Figure 10 It is the HE staining diagram of the positive control specimen, where (a) is the HE staining diagram of the skin histology of 7-day-old mice, (b) is the HE staining diagram of the skin histology of 14-day-old mice, (c) is the HE staining diagram of the skin histology of 28-day-old mice, and (d) is the HE staining diagram of the skin histology of 56-day-old mice;

[0199] Figure 11 It is the HE staining diagram of the negative control specimen, where (a) is the HE staining diagram of the skin histology of 7-day-old mice, (b) is the HE staining diagram of the skin histology of 14-day-old mice, (c) is the HE staining diagram of the skin histology of 28-day-old mice, and (d) is the HE staining diagram of the skin histology of 56-day-old mice.

[0200] From Figures 6 to 11 it can be found that

[0201] Through the histological examination of the back skin of mice, it was found that in D7, D14, and D28, the negative control group showed a downward trend throughout the experimental period, and the number of newly formed hair follicles reached the peak at D7; while in specimen 3# and the high-dose group, the number of newly formed hair follicles reached the maximum at D14, and the number of newly formed hair follicles was 0 at D28; the number of newly formed hair follicles in the positive control group reached the peak at D7 and showed a downward trend throughout the experimental process.

[0202] The number of newly formed hair follicles in the negative control group at D7 and D14 was lower than that in specimen 3# and the positive control group, and the number of newly formed hair follicles in the negative control group was 0 at D28;

[0203] The number of newly formed hair follicles in the low-dose group at D7 and D14 was basically the same as that in the negative control group, and the number of newly formed hair follicles at D28 was higher than that in specimen 3# and the negative control group;

[0204] 3# The number of newly generated hair follicles in D7, D14, and D28 was significantly higher than that in the negative control group. The number of newly generated hair follicles in D7 was 48.40% higher than that in the negative control group, and the number of newly generated hair follicles in D14 reached the peak, which was 218.00% higher than that in the negative control group.

[0205] In the high-dose group, the number of newly generated hair follicles in D7 and D14 was higher than that in the negative control group. The number of newly generated hair follicles in D7 was 19.36% higher than that in the negative control group, the number of newly generated hair follicles in D14 was 32.15% higher than that in the negative control group, and the number of newly generated hair follicles in D28 and the negative control group was 0.

[0206] In the positive control group, the peak was reached in D7, and then showed a downward trend after D14. The number of newly generated hair follicles in D28 and the negative control group was 0.

[0207] In summary, the composition of the present invention has no toxic and side effects, can promote the metabolism and proliferation of hair follicle cells, promote the generation of new hair follicles, shorten the time for hair follicles to enter the growth phase from the resting phase, has a significant promoting effect on hair growth, and helps to strengthen the fastening degree of hair on the scalp, so as to achieve the purpose of preventing hair loss and fixing hair.

[0208] The above is only the preferred specific implementation mode of the present invention, and the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A composition with the function of promoting hair growth and strengthening hair, characterized in that, The raw materials of the composition having the function of hair growth and hair strengthening include, by weight: The protein comprises small molecule peptides and amino acids; the small molecule peptides comprise 500-1500 parts of bovine collagen peptides and 500-1500 parts of hydrolyzed collagen peptides by weight; the amino acids comprise 20-50 parts of taurine by weight; The nutritional enhancers are vitamin enhancers and mineral enhancers; The vitamin fortifier includes, by weight, 0.2 to 10 parts of vitamin A, 10 to 50 parts of vitamin C, 1 to 10 parts of vitamin E, and 10 to 50 parts of yeast powder; the mineral fortifier includes, by weight, 1 to 10 parts of ferrous fumarate and 0.2 to 10 parts of zinc oxide; The powdered phytosterol ester is β-sitosterol ester; The millet extract is prepared by crushing millet, adding water to extract, concentrating the extract, and freeze-drying to obtain the millet extract; The flavoring agent comprises 500-1200 parts of erythritol and 300-600 parts of grapefruit powder in parts by weight.

2. A preparation method of the composition with hair growth and hair strengthening functions according to claim 1, characterized in that, The following steps are involved: (1) Weigh each raw material by weight and sieve for later use; (2) mixing the nutritional enhancer and flavoring agent weighed in step (1) to obtain mixed raw material A; (3) placing the protein and functional ingredients weighed in step (1) in a granulator and stirring, granulating with alcohol as a binder, then fluidizing and drying, and granulating with a granulator to obtain a mixed raw material B; (4) Mixing the mixed raw material A obtained in step (2) and the mixed raw material B obtained in step (3), and sieving them to obtain the composition having the function of hair growth and hair fixation.

3. The preparation method according to claim 2, characterized in that, In step (1), the sieving is through a 40-100 mesh sieve; in step (2), the mixing is equal and incremental mixing; in step (3), the stirring speed is 200-500 rpm; the alcohol dosage is 280-480 parts by mass of 70-95% alcohol sprayed within 2-10 minutes; the fluidized bed drying temperature is 51-58° C., and the drying time is 25-35 minutes; the granulator is a 20-mesh granulator; in step (4), the sieving is through a 40-100 mesh sieve.

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