A kind of Paenibacillus vini bacteria and its application in fermentation preparation of acidic organic water-soluble fertilizer

Abstract

The invention discloses a Paenibacillus vini bacterium and its application in fermentation preparation of acidic organic water-soluble fertilizer. The bacterium is preserved in the Guangdong Microbial Culture Collection Center with a preservation number of GDMCC No: 62513. The invention ferments biogas slurry with more harmful substances, high species and rate of foreign bacteria, certain odor, easy flatulence, and weak alkaline pH value between 7.0 and 8.0 to form a biogas slurry containing a variety of small molecular organic acids, a pH value between 4.5 and 5.5, no flatulence, no odor, and a low rate of foreign bacteria (with Paenibacillus HNP12 (Paenibaciplus vini) as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibacillus HNP12 (Paenibaciplus vini) reaches 10 9 cells / mL, the concentration of Bacillus was 10 7 cells / mL) of acidic organic water-soluble fertilizer.

Description

Technical Field

[0001] The invention relates to the field of microbial application, and in particular to a Paenibacillus vini bacterium, an acidic organic water-soluble fertilizer, a method for preparing the acidic organic water-soluble fertilizer, and a soil treatment method. Background Art

[0002] The organic waste is fermented into biogas through anaerobic fermentation process, and the generated biogas is used for electricity or heat supply. The existing treatment method of biogas slurry or biogas residue is to directly apply it to crops as fertilizer. However, this kind of biogas slurry and biogas residue fertilizer contains more harmful substances, high rate of foreign bacteria, certain odor, easy bloating and weak alkalinity. Large-scale abuse can easily lead to more serious consequences, and it can easily cause problems such as seedling and root burning of crops, and it can easily breed a large number of harmful microorganisms in the soil, leading to the aggravation of soil-borne diseases. Summary of the invention

[0003] Aiming at the problems that biogas slurry has more harmful substances, high rate of foreign bacteria, certain peculiar smell, easy to bloat, weak alkalinity, and is easy to cause root and seedling burns when applied to crops in large quantities as fertilizer, the present invention provides Paenibacillus vini. When the Paenibacillus vini is used to produce organic water-soluble fertilizer, the biogas slurry with more harmful substances, high rate of foreign bacteria, certain peculiar smell, easy to bloat and weak alkalinity is fermented to form an acidic organic water-soluble fertilizer containing multiple small molecular organic acids such as acetic acid, propionic acid and butyric acid, with a pH value between 4.5 and 5.5, without bloating, peculiar smell and low rate of foreign bacteria (with Paenibacillus HNP12 (Paenibaciplus vini) as the dominant strain and containing a small amount of Bacillus).

[0004] A Paenibacillus vini bacterium is deposited in Guangdong Province Microbiological Culture Collection Center with a deposit number of GDMCC No: 62513.

[0005] In one or more specific embodiments of the present application, the Paenibacillus vini is used for the production of organic water-soluble fertilizer.

[0006] Based on the above-mentioned Paenibacillus vini bacteria, the present application also provides an acidic organic water-soluble fertilizer.

[0007] An acidic organic water-soluble fertilizer, which is mainly made of the following ingredients:

[0008] Biogas slurry;

[0009] A bacterial suspension, wherein the amount of the bacterial suspension is 1 ml / 1 L of biogas slurry, and the bacterial suspension contains the Paenibacillus vini bacteria according to claim 1;

[0010] Carbon source, the amount of carbon source is 15-40g / 1L biogas slurry;

[0011] Nitrogen source: the amount of nitrogen source is 10-30g / 1L biogas slurry;

[0012] Beef extract or yeast powder, the amount of beef extract or yeast powder is 3-5g / 1L biogas slurry; and

[0013] Sodium chloride: the amount of sodium chloride is 1-5g / 1L biogas slurry.

[0014] In one or more specific embodiments of the present application, the biogas slurry is a black liquid with a certain odor, easy to bloat and a high rate of miscellaneous bacteria. Its physical and chemical indicators are pH 7.0-8.0, weak alkalinity, nitrogen, phosphorus and potassium content of 10g / L-50g / L, organic matter content of 15g / L-70g / L, density of 1.05-1.10g / mL, and miscellaneous bacteria include methanogens, hydrogen sulfide bacteria, lactic acid bacteria, yeast, cellulose decomposing bacteria, Bacillus, and Paenibacillus, wherein the content of methanogens and Bacillus reaches 10 8 cells / mL, the contents of yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria and lactic acid bacteria each reached 10 7 The concentration of cells / mL.

[0015] In one or more specific embodiments of the present application, the acidic organic water-soluble fertilizer contains small molecule organic acid, pH value 4.5-5.5, no flatulence, no odor, the Paenibacillus vini described in claim 1 is the dominant strain, and the concentration reaches 10 9 cells / mL, the concentration of Bacillus was 10 7 cells / mL.

[0016] In one or more specific embodiments of the present application, the small molecule organic acid is one or more of acetic acid, propionic acid, butyric acid, and citric acid.

[0017] In one or more specific embodiments of the present application, the carbon source is one or more of glucose, starch, biochemical potassium fulvic acid, molasses, and brown sugar.

[0018] In one or more specific embodiments of the present application, the nitrogen source is one or more of peptone, soybean meal powder, bran powder, complex amino acid raw powder, and urea.

[0019] Based on the above-mentioned acidic organic water-soluble fertilizer, the present application also provides a method for producing the acidic organic water-soluble fertilizer.

[0020] A method for preparing the above-mentioned acidic organic water-soluble fertilizer comprises the following steps:

[0021] ①Take biogas slurry;

[0022] ② Add carbon source, nitrogen source, beef extract (or yeast powder), sodium chloride and bacterial suspension to the biogas slurry and mix to obtain an initial mixed solution;

[0023] ③ The initial mixed solution is cultured in a shaking table to obtain a fermentation solution, which is an acidic organic water-soluble fertilizer.

[0024] In one or more specific embodiments of the present application, the shaking incubator culture conditions are: 37° C. culture for 48 hours.

[0025] Based on the above-mentioned acidic organic water-soluble fertilizer, the present application also provides a soil treatment method.

[0026] A soil treatment method comprises: applying the above acidic organic water-soluble fertilizer to the soil when fertilizing crops in the soil.

[0027] In one or more specific embodiments of the present application, the soil is alkaline soil.

[0028] Invention principle and beneficial effects:

[0029] The Paenibacillus vini of the present application is used for the production of organic water-soluble fertilizer. After secondary fermentation of biogas slurry with more harmful substances, high rate of foreign bacteria, certain peculiar smell, easy flatulence and weak alkalinity by Paenibacillus vini, an organic water-soluble fertilizer with beneficial bacteria as dominant strains, stable properties, no peculiar smell, no flatulence, less harmful substances and acidity is obtained. The acidic organic water-soluble fertilizer can be used to adjust the pH of alkaline soil and improve alkaline soil, which is of great significance in agricultural production.

[0030] The present invention can not only effectively solve the problem of secondary pollution of the environment and soil caused by applying biogas slurry as fertilizer to farmland, but also utilize biogas slurry fermentation to prepare acidic organic water-soluble fertilizer. The obtained acidic organic water-soluble fertilizer is rich in acetic acid, propionic acid, butyric acid, citric acid and other small molecule organic acids, and can achieve the purpose of improving alkaline soil by adjusting the pH value of alkaline soil, which is of great significance in alkaline soil improvement and agricultural production.

[0031] Biological Deposit Description

[0032] The preserved bacteria was classified and named Paenibacillus vini, and was deposited in the Guangdong Microbial Culture Collection Center on June 2, 2022 (address: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Institute of Microbiology, Guangdong Academy of Sciences, Postal Code: 510070), with the preservation number GDMCC No: 62513, for the production of organic water-soluble fertilizers.

[0033] Glossary

[0034] Biogas slurry - In the present invention, biogas slurry refers to a black liquid with a certain odor, easy to bloat (bloating occurs after being canned in a plastic bottle and placed in a 37°C incubator for 24 hours) and a high rate of foreign bacteria, which is obtained by anaerobic fermentation of various organic wastes and other wet garbage, and then mainly decomposed by various beneficial and harmful bacteria in nature, such as methanogens, cellulose decomposing bacteria, hydrogen sulfide bacteria, yeast, lactic acid bacteria Bacillus, Paenibacillus, etc. The liquid contains a variety of bacteria, including methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus, etc., and the content of methanogens and Bacillus reaches up to 10 8 cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each with a content of 10 7 The concentration of cells / mL. The physical and chemical indicators of biogas slurry are pH value between 7.0 and 8.0, weakly alkaline, nitrogen, phosphorus and potassium content between 10g / L and 50g / L, organic matter content between 15g / L and 70g / L, and density between 1.05 and 1.10g / mL. DETAILED DESCRIPTION

[0035] The present invention will be further described below in conjunction with embodiments.

[0036] Example 1

[0037] Bacillus HNP12 was isolated from rotten, smelly and sour tomatoes. The Bacillus HNP12 was deposited in the Guangdong Provincial Microbiological Culture Collection Center on June 2, 2022, with the deposit number GDMCC No: 62513, and the deposited bacteria was classified and named Paenibacillus vini.

[0038] Bacillus HNP12 is a type of Bacillus with strong acid production ability, fast growth rate, strong environmental adaptability and strong stress resistance. It can ferment and produce acid in the corresponding liquid culture medium, so that the pH value of the fermentation liquid is reduced from the initial 7.0-8.0 to a minimum of about 4.0, and the fermentation products contain a variety of small molecule organic acids such as acetic acid, propionic acid, butyric acid and citric acid.

[0039] The bacteria with the deposit number of GDMCC No: 62513 in this example were cultured on nutrient agar plates for 24 hours, and then the colonies of HNP12 were picked out and mixed with sterile water to prepare a concentration of 10 8 The bacterial suspension of 10 cells / mL is set aside for use.

[0040] Example 2

[0041] ①Take 1L of biogas slurry.

[0042] ② Add 20g of glucose, 10g of peptone, 5g of sodium chloride, 3g of beef extract (or yeast powder) and 1mL of the bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The pH value of the initial mixed solution is between 7.8 and 8.0, it is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8 The concentration of cells / mL was the highest, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each with a content of 10 7 cells / mL concentration).

[0043] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0044] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 5.0 and 5.2. The acidic organic water-soluble fertilizer finally obtained contains a variety of small molecular organic acids such as acetic acid, propionic acid, butyric acid, etc., with a pH value of between 5.0 and 5.2, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 10 7 cells / mL) of acidic organic water-soluble fertilizer).

[0045] Example 3

[0046] ①Take 1L of biogas slurry.

[0047] ② Add 15g of glucose, 10g of starch, 10g of peptone, 5g of sodium chloride, 3g of beef extract (or yeast powder) and 1mL of the bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The pH value of the initial mixed solution is between 7.8 and 8.0, it is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8 The concentration of cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each reached 10 7 cells / mL concentration).

[0048] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0049] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 4.6 and 4.8. The acidic organic water-soluble fertilizer finally obtained contains small molecular organic acids such as acetic acid, propionic acid, butyric acid and citric acid, with a pH value between 4.6 and 4.8, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 10 7 cells / mL) of acidic organic water-soluble fertilizer, ) of acidic organic water-soluble fertilizer.

[0050] Example 4

[0051] ①Take 1L of biogas slurry.

[0052] ② Add 25 g of biochemical potassium fulvic acid, 20 g of soybean meal powder, 5 g of sodium chloride, 3 g of beef extract (or yeast powder) and 1 mL of the bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The pH value of the initial mixed solution is between 7.0 and 7.2, and the solution is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8 The concentration of cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each reached 10 7 cells / mL concentration).

[0053] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0054] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 4.6 and 4.8. The acidic organic water-soluble fertilizer finally obtained contains a variety of small molecular organic acids such as acetic acid, propionic acid, butyric acid, etc., with a pH value between 4.6 and 4.8, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 10 7 cells / mL) of acidic organic water-soluble fertilizer).

[0055] Example 5

[0056] ①Take 1L of biogas slurry.

[0057] ② Add 25g molasses, 5g composite amino acid powder, 5g sodium chloride, 3g beef extract (or yeast powder) and 1mL bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The pH value of the initial mixed solution is between 7.0 and 7.2, it is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8 The concentration of cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each reached 10 7 cells / mL concentration).

[0058] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0059] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 4.5 and 4.7. The acidic organic water-soluble fertilizer finally obtained contains a variety of small molecular organic acids such as acetic acid, propionic acid, butyric acid, etc., with a pH value of between 4.5 and 4.7, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 10 7 cells / mL) of acidic organic water-soluble fertilizer).

[0060] Example 6

[0061] ①Take 1L of biogas slurry.

[0062] ② Add 25g brown sugar, 20g bran powder, 5g sodium chloride, 3g beef extract (or yeast powder) and 1mL bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The pH value of the initial mixed solution is between 7.5 and 7.7, and the solution is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8 The concentration of cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each reached 10 7 cells / mL concentration).

[0063] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0064] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 4.6 and 4.8. The acidic organic water-soluble fertilizer finally obtained contains a variety of small molecular organic acids such as acetic acid, propionic acid, butyric acid, etc., with a pH value between 4.6 and 4.8, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 10 7cells / mL) of acidic organic water-soluble fertilizer).

[0065] Example 7

[0066] ①Take 1L of biogas slurry.

[0067] ② Add 20g brown sugar, 5g starch, 10g urea, 5g sodium chloride, 3g beef extract (or yeast powder) and 1mL bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The initial mixed solution has a pH value of 7.8 to 8.0, is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8 The concentration of cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each reached 10 7 cells / mL concentration).

[0068] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0069] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 5.3 and 5.5. The acidic organic water-soluble fertilizer finally obtained contains a variety of small molecular organic acids such as acetic acid, propionic acid, butyric acid, etc., with a pH value of between 5.3 and 5.5, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 10 7 cells / mL) of acidic organic water-soluble fertilizer).

[0070] Example 8

[0071] ①Take 1L of biogas slurry.

[0072] ② Add 10g of glucose, 10g of starch, 10g of soybean meal powder, 5g of composite amino acid powder, 5g of sodium chloride, 3g of beef extract (or yeast powder) and the bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The pH value of the initial mixed solution is between 7.2 and 7.4, it is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8The concentration of cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each reached 10 7 cells / mL concentration).

[0073] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0074] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 4.7 and 4.9. The acidic organic water-soluble fertilizer finally obtained contains a variety of small molecular organic acids such as acetic acid, propionic acid, butyric acid, etc., with a pH value of between 4.7 and 4.9, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 10 7 cells / mL) of acidic organic water-soluble fertilizer).

[0075] Example 9

[0076] ①Take 1L of biogas slurry.

[0077] ② Add 15g of glucose, 5g of starch, 5g of brown sugar, 10g of soybean meal powder, 5g of composite amino acid powder, 5g of urea, 5g of sodium chloride, 3g of beef extract (or yeast powder) and 1mL of the bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The initial mixed solution has a pH value of 7.6 to 7.8, is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8 The concentration of cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each reached 10 7 cells / mL concentration).

[0078] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0079] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 4.6 and 4.8. The acidic organic water-soluble fertilizer finally obtained contains a variety of small molecular organic acids such as acetic acid, propionic acid, butyric acid, etc., with a pH value between 4.6 and 4.8, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 10 7 cells / mL) of acidic organic water-soluble fertilizer).

[0080] Experiment 10

[0081] ①Take 1L of biogas slurry.

[0082] ② Add 15g of glucose, 15g of brown sugar, 5g of biochemical potassium humate, 5g of peptone, 5g of bran powder, 5g of soybean meal powder, 5g of sodium chloride, 3g of beef extract (or yeast powder) and 1mL of the bacterial suspension of Example 1 to the biogas slurry, and mix to obtain an initial mixed solution. The pH value of the initial mixed solution is between 7.4 and 7.6, it is easy to swell, has a certain odor, and has a high rate of miscellaneous bacteria (containing methanogens, hydrogen sulfide bacteria, cellulose decomposing bacteria, lactic acid bacteria, yeast, Bacillus and other bacteria, among which the content of methanogens and Bacillus is as high as 10 8 The concentration of cells / mL, followed by yeast, cellulose decomposing bacteria, hydrogen sulfide bacteria, lactic acid bacteria, etc., each reached 10 7 cells / mL concentration).

[0083] ③ The initial mixed solution was cultured in a shaking incubator at 37°C for 48 hours to obtain fermentation liquid (i.e., acidic organic water-soluble fertilizer).

[0084] In this step, Paenibaciplus vini is used to fully ferment and produce acid, so that the pH value of the fermentation liquid is reduced to between 4.5 and 4.7. The acidic organic water-soluble fertilizer finally obtained contains a variety of small molecular organic acids such as acetic acid, propionic acid, butyric acid, etc., with a pH value of between 4.5 and 4.7, no flatulence (after being canned in a plastic bottle, it is placed in a 37°C incubator for 48 hours without flatulence), no odor, and a low rate of miscellaneous bacteria (with Paenibaciplus vini as the dominant strain, containing a small amount of bacillus, and the concentration of Paenibaciplus vini reaches 10 9 cells / mL, the concentration of Bacillus was reduced to 107 cells / mL) of acidic organic water-soluble fertilizer).

[0085] From Examples 1-10, it can be seen that:

[0086] (1) The biogas slurry is subjected to secondary fermentation by using the Paenibacillus HNP12 (the deposit number is GDMCC No: 62513, and the deposited bacteria is classified and named Paenibacillus vini) of Example 1, which can effectively solve the problems that the biogas slurry is directly applied to farmland as a fertilizer, which is easy to cause crop root and seedling burns, easy to breed the growth and reproduction of harmful microorganisms, and aggravate soil-borne diseases. The biogas slurry can also be recycled to reduce the secondary pollution caused by the biogas slurry to the environment and soil.

[0087] (2) The present invention uses Paenibacillus vini (GDMCC No. 62513) with high activity and strong acid production ability to perform secondary fermentation on biogas slurry with high bacteria rate, pH value of 7.0-8.0, certain odor, easy flatulence and more harmful substances. The obtained organic water-soluble fertilizer has Paenibacillus vini as the dominant strain, the pH value is reduced to 4.5-5.5, the property is stable, there is no odor, no flatulence and less harmful substances. The acidic organic water-soluble fertilizer is rich in acetic acid, propionic acid, butyric acid, citric acid and other small molecules.

[0088] Organic acids can be used as soil conditioners to adjust the pH of alkaline soil, thereby achieving the purpose of improving alkaline soil. They are of great significance in agricultural production.

[0089] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A Paenibacillus vini Bacteria, the Paenibacillus vini The bacteria are deposited in Guangdong Microbiological Culture Collection Center with the deposit number GDMCC No: 62513.

2. An acidic organic water-soluble fertilizer, characterized in that: The acidic organic water-soluble fertilizer is mainly made of the following ingredients: Biogas slurry; Bacterial suspension, the amount of bacterial suspension is 1ml / 1L biogas slurry, and the bacterial suspension contains the Paenibacillus vini bacteria; Carbon source, the amount of carbon source is 15~40g / 1L biogas slurry; Nitrogen source, the amount of nitrogen source is 10~30g / 1L biogas slurry; Beef extract or yeast powder, the amount of beef extract or yeast powder is 3~5 g / 1L biogas slurry; as well as Sodium chloride, the amount of sodium chloride is 1~5g / 1L of biogas slurry.

3. The acidic organic water-soluble fertilizer according to claim 2, characterized in that, Said Paenibacillus vini The amount of bacteria is 10 8 cells / 1L biogas slurry.

4. The acidic organic water-soluble fertilizer according to claim 2, characterized in that The biogas slurry is black, has a certain odor, is easy to bloat, and has a high rate of miscellaneous bacteria. Its physical and chemical indicators are pH value 7.0-8.0, weak alkalinity, nitrogen, phosphorus and potassium content 10g / L-50g / L, organic matter content 15g / L-70g / L, density 1.05-1.10g / mL, and miscellaneous bacteria include methanogens, hydrogen sulfide bacteria, lactic acid bacteria, yeast, and bacillus, among which the content of methanogens and bacillus reaches 10 8 cells / mL, the contents of yeast, hydrogen sulfide bacteria and lactic acid bacteria each reached 10 7 The concentration of cells / mL.

5. The acidic organic water-soluble fertilizer according to any one of claims 2 to 4, characterized in that: The acidic organic water-soluble fertilizer contains small-molecule organic acid, has a pH value of 4.5 to 5.5, does not cause flatulence, and has no peculiar smell.

6. The acidic organic water-soluble fertilizer according to claim 5, characterized in that The small molecule organic acid is one or more of acetic acid, propionic acid, butyric acid and citric acid; or / and The carbon source is one or more of glucose, starch, biochemical potassium fulvic acid, molasses, and brown sugar; or / and The nitrogen source is one or more of peptone, soybean meal powder, bran powder, composite amino acid powder and urea.

7. A method for preparing the acidic organic water-soluble fertilizer according to any one of claims 2 to 6, comprising the following steps: ①Take biogas slurry; ② Add carbon source, nitrogen source, beef extract or yeast powder, sodium chloride and bacterial suspension to the biogas slurry, and mix to obtain an initial mixed solution, wherein the bacterial suspension contains the Paenibacillus vini bacteria; ③ The initial mixed solution is cultured in a shaking table to obtain a fermentation liquid, which is an acidic organic water-soluble fertilizer.

8. The method for preparing the acidic organic water-soluble fertilizer according to claim 7, characterized in that: The shaking incubation conditions are: 37° C. for 48 h.

9. A soil treatment method, comprising: When fertilizing crops in the soil, the acidic organic water-soluble fertilizer according to any one of claims 2 to 6 is applied to the soil.

10. The soil treatment method according to claim 9, characterized in that the soil is alkaline soil.