A composite microbial preparation for water treatment

Abstract

The present invention belongs to the technical field of microbial application, and specifically relates to a composite microbial preparation for water treatment, including Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus dysvalley YZS-C10, Bacillus pumilus YZS-C11 and Bacillus arfaye YZS-D08. The composite microbial preparation for water treatment is prepared by compounding the screened Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus dysvalley YZS-C10, Bacillus pumilus YZS-C11 and Bacillus arfaye YZS-D08 with strong COD degradation effect. The composite microbial preparation for water treatment is rich in beneficial bacteria, and can effectively reduce the COD concentration in the water body through the synergistic effect of multiple strains, thereby achieving the purpose of purifying water quality.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial application, and in particular relates to a composite microbial preparation for water treatment. Background Art

[0002] Rivers and lakes are important components of the urban living environment. When the COD content in the water exceeds the normal content, and when domestic sewage and industrial sewage are eutrophic, bacteria will cause a large amount of dissolved oxygen consumption in the water, causing the water to be hypoxic and black and smelly. Composite microbial preparations are made by screening targeted bacterial species to form composite microbial agents, so that they can play a synergistic role in the microbial sewage treatment system, quickly form dominant bacterial communities, decompose, absorb and transfer pollutants in rivers and other water bodies, thereby achieving the best treatment effect without causing secondary pollution; they are safe, efficient, low-cost and easy to operate. Summary of the invention

[0003] The purpose of the present invention is to provide a composite microbial preparation for water treatment. The composite microbial preparation can effectively reduce the COD concentration in water through the synergistic promotion of multiple microorganisms, thereby achieving the purpose of purifying water quality.

[0004] To achieve the above object, the present invention adopts the following technical solutions:

[0005] The present invention first provides a water treatment composite microbial preparation, which comprises Bacillus tequila YZS-C01, Bacillus shafusus YZS-C03, Bacillus valerianus YZS-C10, Bacillus pumilus YZS-C11 and Bacillus arnoldii YZS-D08.

[0006] Among them, the Bacillus equilensis YZS-C01 is classified and named Bacillus equilensis (Bacillus equilensis), and was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on July 9, 2021. The deposit address is: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is: CGMCC No. 22856.

[0007] Among them, the Bacillus safensis YZS-C03, whose classification name is Bacillus safensis, was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on February 1, 2021. The deposit address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is: CGMCC No. 21798.

[0008] Among them, the Bacillus vallismortis YZS-C10, whose classification name is Bacillus vallismortis (Bacillusvallismortis), was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on July 9, 2021. The deposit address is: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is: CGMCC No. 22857.

[0009] Among them, the Bacillus pumilus YZS-C11, whose classification name is Bacillus pumilus, was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on July 9, 2021. The deposit address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is: CGMCC No. 22858.

[0010] Among them, the Bacillus aryabhattai YZS-D08, whose classification name is Bacillus aryabhattai, was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on February 1, 2021. The deposit address is: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is: CGMCC No. 21797.

[0011] The colony characteristics and bacterial morphology of the above-mentioned Bacillus tequila YZS-C01 are as follows:

[0012] The colonies formed by strain YZS-C01 after culturing on NA plates for 24 hours are light yellow, translucent, dull, round, and non-fluid, with a colony diameter of 4-5 mm; the bacterial body morphology is rod-shaped, Gram-positive, with spores and no capsule.

[0013] The physiological and biochemical characteristics of the above-mentioned Bacillus tekila YZS-C01 are:

[0014] Catalase reaction was negative, VP assay was negative, MR assay was negative, glucose acid production test was positive, glucose gas production test was negative, citrate test was negative, nitrate reduction was positive, starch hydrolysis was positive, indole test was negative, malonic acid assay was negative, and H2S production test was positive.

[0015] The 16S rDNA sequence (SEQ ID NO.1) of the above-mentioned Bacillus tequilensis YZS-C01 was compared with the sequence in the GenBank database. The results showed that YZS-C01 and Bacillus tequilensis were on the same branch, and the similarity of its 16S rDNA sequence with Bacillus tequilensis (KY819004.1) was 100%. Combined with the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, it was preliminarily identified as Bacillus tequilensis.

[0016] The colony characteristics and bacterial morphology of the above-mentioned Bacillus sabdariffa YZS-C03 are as follows:

[0017] The colonies formed after culturing Bacillus shaforniensis YZS-C03 on NA plate medium for 24 hours are light yellow, dull, round, non-fluid, Gram-positive, with spores and no capsule; the colony diameter is 4-5 mm.

[0018] Physiological and biochemical characteristics of the above-mentioned Bacillus sabdariffa YZS-C03:

[0019] Catalase reaction was negative, VP assay was negative, MR assay was negative, glucose acid production test was negative, glucose gas production test was negative, citrate test was negative, nitrate reduction was positive, starch hydrolysis was positive, indole test was negative, malonic acid assay was negative, and H2S production test was negative.

[0020] The 16S rDNA sequence of the above Bacillus safensis was compared with the sequence in the GenBank database. The results showed that YZS-C03 and Bacillus safensis were on the same branch, and its 16S rDNA sequence was 100% similar to Bacillus safensis (MT501806.1). Combining the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, it was preliminarily identified as Bacillus safensis.

[0021] The colony characteristics and bacterial morphology of the above-mentioned Bacillus dysvalleyi YZS-C10 are as follows:

[0022] The colonies formed after the YZS-C10 strain was cultured on the NA plate medium for 24 hours were light yellow, dull, round, non-fluid, Gram-positive, with spores and no capsule; the colony diameter was 3-4 mm.

[0023] Physiological and biochemical characteristics of the above-mentioned Bacillus spp. YZS-C10:

[0024] Catalase reaction was negative, VP assay was positive, MR assay was negative, glucose acid production test was positive, glucose gas production test was negative, citrate test was negative, nitrate reduction was positive, starch hydrolysis was positive, indole test was negative, malonic acid assay was negative, and H2S production test was positive.

[0025] The 16S rDNA sequence of the above-mentioned Bacillus vallismortis YZS-C10 was compared with the sequence in the GenBank database. The results showed that YZS-C10 and Bacillus vallismortis were on the same branch, and its 16S rDNA sequence was 100% similar to Bacillus vallismortis (KP994551.1). Combined with the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, it was preliminarily identified as Bacillus vallismortis.

[0026] The colony characteristics and bacterial morphology of the Bacillus pumilus YZS-C11 are as follows:

[0027] After YZS-C11 was cultured on NA plates for 24 hours, the colonies formed were light yellow, dull, round, non-fluid, Gram-positive, with spores but no capsule; the colony diameter was 3-5 mm.

[0028] Physiological and biochemical characteristics of the Bacillus pumilus YZS-C11:

[0029] Catalase reaction was negative, VP assay was negative, MR assay was negative, glucose acid production test was negative, glucose gas production test was negative, citrate test was negative, nitrate reduction was positive, starch hydrolysis was positive, indole test was negative, malonic acid assay was negative, and H2S production test was negative.

[0030] The 16S rDNA sequence of the Bacillus pumilus was compared with the sequence in the GenBank database, and the results showed that YZS-C11 and Bacillus pumilus were on the same branch, and the similarity between its 16S rDNA sequence and Bacillus pumilus (MN581181.1) was 100%. Combining the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, it was preliminarily identified as Bacillus pumilus.

[0031] The colony characteristics and bacterial morphology of the Bacillus arifugeyi YZS-D08 are as follows:

[0032] After YZS-D08 was cultured on NA plates for 24 hours, the colonies formed were light yellow, translucent, dull, round, non-fluid, Gram-positive, with spores and no capsule; the colony diameter was 4-5 mm.

[0033] Physiological and biochemical characteristics of the Bacillus argyi:

[0034] The catalase reaction was negative, the VP assay was negative, the MR assay was negative, the glucose acid production test was negative, the glucose gas production test was negative, the citrate test was negative, the nitrate reduction was positive, the starch hydrolysis was positive, the indole test was negative, the malonic acid assay was negative, and the H2S production test was positive.

[0035] The 16S rDNA sequence of Bacillus aryabhattai YZS-D08 was compared with the sequence in the GenBank database, and the results showed that YZS-D08 and Bacillus aryabhattai were on the same branch, and the similarity between its 16S rDNA sequence and Bacillus aryabhattai (MN062938.1) was 100%. Combining the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, it was preliminarily identified as Bacillus aryabhattai.

[0036] Furthermore, the mass ratio of solid bacterial powder or fermentation liquid of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus agglomerans YZS-D08 in the water treatment composite microbial preparation is (1-3): (1-3): (1-3): (1-3): (2-5).

[0037] Furthermore, the total live bacteria concentration in the water treatment composite microbial preparation is (1-3)×10 9 cfu / ml or (1~3)×10 9 cfu / g.

[0038] Furthermore, the water treatment composite microbial preparation is a liquid preparation or a solid preparation.

[0039] Furthermore, the preparation method of the solid preparation of the water treatment composite microbial preparation is as follows: solid bacterial powders of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus aggregatus YZS-D08 are mixed in a mass ratio to prepare a solid mixed bacterial powder; a carrier is mixed with the solid mixed bacterial powder in a mass ratio of 100:2 to 5 to obtain a solid preparation of the water treatment composite microbial preparation; the carrier is bran and coral sand mixed in any proportion.

[0040] Furthermore, the preparation method of the liquid preparation of the water treatment composite microbial preparation is as follows: the fermentation broths of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus agglomerans YZS-D08 are mixed in a mass ratio of (1-3): (1-3): (1-3): (1-3): (2-5) to obtain the obtained product.

[0041] The beneficial effects of the present invention are:

[0042] The water treatment composite microbial preparation provided by the present invention is compounded by using the screened tequila bacillus YZS-C01, shafu bacillus YZS-C03, death valley bacillus YZS-C10, pumilus YZS-C11, and arnoldii bacillus YZS-D08 with strong COD degradation effect. The water treatment composite microbial preparation is rich in beneficial bacteria and can effectively reduce the COD concentration in the water body through the synergistic effect of multiple bacteria species, thereby achieving the purpose of purifying water quality. DETAILED DESCRIPTION

[0043] The present invention is further described below in conjunction with specific embodiments.

[0044] Bacteria materials:

[0045] (1) Bacillus equilensis YZS-C01, whose classification name is Bacillus equilensis, was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on July 9, 2021, with the deposit address at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, with the deposit number: CGMCC No. 22856, and the deposit address: Beijing, China, which has been made public.

[0046] (2) Bacillus safensis YZS-C03, whose classification name is Bacillus safensis, was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on February 1, 2021. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China. The deposit number is: CGMCC No. 21798, and the deposit address is: Beijing, China, which has been made public.

[0047] (3) Bacillus vallismortis YZS-C10, whose classification name is Bacillus vallismortis, was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on July 9, 2021. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China. The deposit number is: CGMCC No. 22857, and the deposit address is: Beijing, China, which has been made public.

[0048] (4) Bacillus pumilus YZS-C11, whose classification name is Bacillus pumilus, was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on July 9, 2021. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China. The deposit number is: CGMCC No. 22858, and the deposit address is: Beijing, China, which has been made public.

[0049] (5) Bacillus aryabhattai YZS-D08, whose classification name is Bacillus aryabhattai, was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on February 1, 2021. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China. The deposit number is: CGMCC No. 21797, and the deposit address is: Beijing, China, which has been made public.

[0050] The invention provides a composite microbial preparation for water treatment, which comprises Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus valerian YZS-C10, Bacillus pumilus YZS-C11 and Bacillus arguably YZS-D08; the mass ratio of solid bacterial powder or fermentation liquid of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus valerian YZS-C10, Bacillus pumilus YZS-C11 and Bacillus arguably YZS-D08 in the composite microbial preparation for water treatment is (1-3): (1-3): (1-3): (1-3): (2-5); the total live bacteria concentration in the composite microbial preparation for water treatment is (1-3)×10 9 cfu / ml or (1~3)×10 9 cfu / g.

[0051] In the present invention, the water treatment composite microbial preparation is preferably a solid preparation or a liquid preparation. When the water treatment composite microbial preparation is a solid preparation, the water treatment composite microbial preparation includes a carrier. In the present invention, the carrier is bran and coral sand. The total mass ratio of the carrier bran and coral sand to Bacillus tequila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus arguably YZS-D08 is preferably 100: (2 to 5). In the present invention, when the water treatment composite microbial preparation is a solid preparation, the fermentation broth of Bacillus tequila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus arguably YZS-D08 is prepared into solid bacterial powder by spray drying, and then mixed in proportion.

[0052] In the present invention, when the water treatment composite microbial preparation is a liquid preparation, it is obtained by mixing the fermentation broths of Bacillus tekila YZS-C01, Bacillus shafusus YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus agglomerans YZS-D08 in a mass ratio of (1-3): (1-3): (1-3): (1-3): (2-5).

[0053] In the present invention, the preparation method of the solid bacterial powder preferably includes the following steps: activating the bacterial species and then conducting continuous two-stage seed liquid culture, inoculating the bacterial species for fermentation and concentrating to obtain a concentrated bacterial liquid, mixing the fermentation liquid of the Bacillus tequila YZS-C01, Bacillus shafusus YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus arnoldii YZS-D08 with light calcium carbonate and spray-drying the mixture to obtain the solid bacterial powder.

[0054] In the present invention, the strain is inoculated into NB culture medium and cultured for 15 to 25 hours to obtain a primary seed solution; the culture temperature is preferably 25 to 35°C, and the culture speed is preferably 150 to 350 r·min -1 .

[0055] After obtaining the primary seed solution, the present invention transfers the primary seed solution to NB liquid culture medium for 10 to 20 hours to obtain the secondary seed solution. In the present invention, the inoculation amount of the primary seed solution is preferably 0.5% to 1.0% (V / V), the culture is preferably carried out in a fermenter, sterile air is introduced during the culture, the fermenter ventilation manually adjusts the speed and air flow to adjust DO (DO40%), the culture temperature is 25°C to 35°C, and the culture speed is 200 to 350 r·min-1 The culturing time is preferably 10 to 20 hours. In the present invention, the OD of the secondary seed solution is 600 It is preferably 0.6 to 1.0.

[0056] After obtaining the secondary seed liquid, the present invention connects the secondary seed liquid to the fermentation medium for fermentation culture to obtain the fermentation liquid. In the present invention, the inoculation amount of the secondary seed liquid is 0.5% to 1.0% (V / V), and the tank pressure during the fermentation culture process is maintained at 0.05MPa; sterile air is introduced during the culture process, the fermentation tank ventilation manually adjusts the speed and air flow to adjust DO (DO40%), the culture temperature is 25°C to 35°C, and the culture speed is 200 to 350r·min -1 The culturing time is preferably 15 to 35 hours. In the present invention, the OD of the secondary seed solution is 600 It is preferably 0.8 to 1.5.

[0057] After obtaining the fermented bacterial liquid, the present invention concentrates the fermented bacterial liquid to obtain a concentrated bacterial liquid. In the present invention, the concentration multiple of the concentrated bacterial liquid is 5 to 20 times; the concentration method is to obtain a concentrated bacterial liquid by filtering the fermented bacterial liquid of Bacillus tequila YZS-C01, Bacillus shafusus YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus arnoldii YZS-D08 through a ceramic membrane. The present invention does not specifically limit the specific parameter settings of the concentration equipment, and conventional parameter settings can be used to achieve the above-mentioned multiple concentration.

[0058] After obtaining the fermented bacterial liquid, the present invention filters the fermented bacterial liquid of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11 and Bacillus arnoldii YZS-D08 through a ceramic membrane to obtain a concentrated bacterial liquid, and after adding light calcium carbonate to the concentrated bacterial liquid, spray-drying is performed to obtain a solid bacterial powder, the mass ratio of the concentrated liquid to the light calcium carbonate is 100:2-5, and the control indicators of the spray drying process are that the material inlet temperature is 150-200°C, the outlet temperature is 80-85°C, and the material is dried in the tower for 15-30S.

[0059] Example 1

[0060] (1) Preparation of Bacillus tergillosis YZS-C01 bacterial liquid and solid bacterial powder:

[0061] Activate the YZS-C01 strain of Bacillus tekila, pick a single colony, inoculate it into 150 ml NB medium (sterilized at 121°C for 30 min), and incubate at 28°C, 180 r·min -1The mixture was shaken and cultured for 20 h under the same conditions to obtain the primary seed solution.

[0062] Preparation of secondary seed solution of Bacillus tekila YZS-C01:

[0063] The first-grade seed liquid was inoculated into a sterile seed tank with an inoculation amount of 0.5% to 1.0% (V / V). The culture conditions were: ventilation was manually adjusted to adjust the speed and air flow to adjust DO (DO40%), the culture temperature was 30℃ to 35℃, and the speed was 200 to 350 r·min -1 , tank pressure 0.05MPa; culture time 15-20h. The OD of the secondary seed solution 600 The value is between 0.6 and 0.8, at which time the strain has the strongest activity.

[0064] Preparation of fermentation broth of Bacillus tequila YZS-C01:

[0065] The secondary seed liquid of Bacillus tekila YZS-C01 is inoculated into a sterile fermentation tank at an inoculation rate of 0.5% to 1.0% (V / V). The composition of the fermentation medium is as follows (per liter): yeast extract: 10-20 grams, maltose syrup: 25-35 grams, magnesium chloride: 0.2-0.5 grams, calcium chloride: 0.1-0.4 grams, manganese chloride: 0.1-0.4 grams, dipotassium hydrogen phosphate: 1.0-2.5 grams. The culture conditions are: ventilation manually adjusts the speed and air flow to adjust DO (DO40%), the culture temperature is 30℃ to 35℃, and the speed is 200 to 350r·min -1 , tank pressure 0.05MPa; culture time 30-35h, pH rises to 7.5-8.0, OD 600 The value is 1.2-1.5. When the spores are more than 90% mature after sampling and microscopic examination, the fermentation is terminated to obtain the mature fermentation liquid.

[0066] Preparation of Bacillus tequila YZS-C01 powder:

[0067] The fermented mature bacterial liquid obtained above was filtered through a ceramic membrane, and then light calcium carbonate was added and mixed evenly. The mass ratio of the concentrated bacterial liquid to the light calcium carbonate was 100:2-5, and a solid-liquid mixture was obtained, which was spray-dried. The spray-drying conditions were: the material inlet temperature was 150-200°C, the outlet temperature was 80-85°C, and the material was dried in the tower for 15-30 seconds. The solid bacterial powder of Bacillus tequila YZS-C01 was obtained, and the moisture content of the bacterial powder was less than 5% (W / W), and the number of viable bacteria was 2-5×10 11 cfu / g.

[0068] (2) The preparation processes of bacterial liquid and solid bacterial powder of Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus alteirah YZS-D08 are the same as those of Bacillus tequila YZS-C01.

[0069] Example 2

[0070] The solid bacterial powders of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus agni YZS-D08 obtained in the above Example 1 were mixed in a mass ratio of 3:3:3:3:5 to obtain solid mixed bacterial powder. Then, the carrier bran and coral sand (the bran and the coral sand were mixed in any mass ratio) were mixed with the above solid mixed bacterial powder in a mass ratio of 100:5 to obtain a solid preparation 1 of a water treatment composite microbial preparation, wherein the total viable bacteria concentration in the water treatment composite microbial preparation was 3×10 9 cfu / g.

[0071] The solid bacterial powders of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus agni YZS-D08 obtained in the above Example 1 were mixed in a mass ratio of 1:1:1:1:2 to obtain solid mixed bacterial powder. Then, the carrier bran and coral sand (the bran and the coral sand were mixed in any mass ratio) were mixed with the above solid mixed bacterial powder in a mass ratio of 100:2 to obtain a solid preparation 2 of a water treatment composite microbial preparation, wherein the total viable bacteria concentration in the water treatment composite microbial preparation was 1×10 9 cfu / g.

[0072] The solid bacterial powders of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus dysvalley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus arguably YZS-D08 obtained in the above Example 1 were mixed uniformly at a mass ratio of 2:2:2:2:3 to obtain solid mixed bacterial powder. Then, the carrier bran and coral sand (the bran and the coral sand were mixed at any mass ratio) were mixed with the above solid mixed bacterial powder at a mass ratio of 100:3 to obtain a solid preparation 3 of a water treatment composite microbial preparation, wherein the total viable bacteria concentration in the water treatment composite microbial preparation was 2×10 9 cfu / g.

[0073] Example 3

[0074] The fermented mature bacterial broths of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11 and Bacillus arnoldii YZS-D08 obtained in the above Example 1 were mixed in a mass ratio of 3:3:3:3:5 to obtain a water treatment composite microbial preparation liquid preparation 1.

[0075] The fermented mature bacterial liquids of the above-mentioned Bacillus tequila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus arnoldii YZS-D08 are mixed in a mass ratio of 1:1:1:1:2 to obtain a water treatment composite microbial preparation liquid preparation 2.

[0076] The fermented mature bacterial broths of Bacillus tekila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11 and Bacillus arnoldii YZS-D08 obtained in the above Example 1 were mixed in a mass ratio of 2:2:2:2:3 to obtain liquid preparation 3 of composite microbial preparation for water treatment.

[0077] Example 4

[0078] Application of the water treatment composite microbial preparation liquid preparation 1 prepared in Example 3 in domestic sewage treatment

[0079] Take a 3L plastic bucket, add 1000mL of domestic sewage (initial index: CODcr 350mg / L), add 20mL of the water treatment composite microbial preparation liquid preparation 1 prepared in Example 3 of the present invention, control the temperature above 20 degrees, put in an aeration head, aerate continuously, measure COD every 24 hours, and measure continuously for 2 days.

[0080] The results showed that after aeration, the transparency of the water body gradually increased with time after the application of the water treatment composite microbial preparation. 24 days after the application of the water treatment composite microbial preparation, the COD of the domestic sewage decreased from the original 350 mg / L to 158 mg / L. After 48 days, the COD of the domestic sewage decreased to 90 mg / L, and the COD degradation rate was 74.2%, which fully demonstrated that the water treatment composite microbial preparation had a significant effect on the COD degradation of domestic sewage.

[0081] Table 1 Degradation effect of water treatment composite microbial preparation on COD

[0082]

[0083] Example 5

[0084] Application of the water treatment composite microbial preparation solid preparation 1 prepared in Example 2 in the treatment of landfill leachate

[0085] A landfill leachate treatment center, designed to handle 100m 3 / d. Influent water quality: COD Cr :8000-10000mg / L. The treatment process is regulating tank-self-cleaning filter-primary sedimentation tank-A / O tank-secondary sedimentation tank-standard discharge. In the aerobic tank, the water volume is 10g / m 3 The water treatment composite microbial preparation solid preparation 1 prepared in Example 2 was applied in an amount of . After the treatment system was stably operated, it can be seen that the effluent COD of the landfill leachate treatment system was reduced from 8146 mg / L of the influent to 485 mg / L, the COD degradation rate was 94.05%, the water transparency increased, the pH decreased, the ammonia nitrogen content decreased, and the biochemical system was strengthened.

[0086] Table 2 Degradation effect of composite microbial preparations for water treatment on COD in landfill leachate

[0087]

[0088] Example 6

[0089] Application of water treatment composite microbial preparation solid preparation 2 prepared in Example 2 in landscape water treatment

[0090] A landscape water body, with a total length of 5km, a width of 20-25m, a water flow rate of 0.3-7.0m / min, a bottom mud depth of 0.5-0.9m, a treated water surface length of 1km, turbid water, black and smelly. Water quality before treatment: COD: 90-120mg / L. Every day, 5g / m 3 The water treatment composite microbial preparation solid preparation 2 prepared in Example 2 was applied in an amount of , and intermittent aeration was performed. After 10 days of continuous treatment, it can be seen that the COD of the treated water body was reduced from 98 mg / L of the original water quality to 32 mg / L, the COD degradation rate was 67.35%, the odor of the water body was greatly reduced, and the transparency was increased.

[0091] Table 3 Degradation effect of water treatment composite microbial preparations on COD in landscape water bodies

[0092]

[0093] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A composite microbial preparation for water treatment, characterized in that: Including Bacillus tequila YZS-C01 ( Bacillus tequilensis )、Bacillus saffron YZS-C03( Bacillus safensis )、Bacillus subtilis YZS-C10( Bacillus vallismortis )、Bacillus pumilus YZS-C11( Bacillus pumilus ) and Bacillus arifugeyi YZS-D08 ( Bacillus aryabhattai ); the deposit number of the Bacillus tequila YZS-C01 is: CGMCC No.22856, the deposit number of the Bacillus shafu YZS-C03 is: CGMCC No.21798, the deposit number of the Bacillus death valley YZS-C10 is: CGMCC No.22857, the deposit number of the Bacillus pumilus YZS-C11 is: CGMCC No.22858, and the deposit number of the Bacillus arnoldii YZS-D08 is: CGMCC No.21797.

2. The water treatment composite microbial preparation according to claim 1, characterized in that: The mass ratio of the solid bacterial powder or fermentation liquid of the Bacillus tequila YZS-C01, Bacillus shafusus YZS-C03, Bacillus valleculata YZS-C10, Bacillus pumilus YZS-C11, and Bacillus agglomerans YZS-D08 is (1-3): (1-3): (1-3): (1-3): (2-5).

3. The water treatment composite microbial preparation according to claim 1, characterized in that: The total live bacteria concentration in the water treatment composite microbial preparation is (1-3)×10 9 cfu / ml or (1~3)×10 9 cfu / g.

4. The water treatment composite microbial preparation according to claim 1, characterized in that: The water treatment composite microbial preparation is a liquid preparation or a solid preparation.

5. The water treatment composite microbial preparation according to claim 1, characterized in that: The preparation method of the water treatment composite microbial preparation solid preparation is as follows: solid bacterial powders of Bacillus tequila YZS-C01, Bacillus shafu YZS-C03, Bacillus death valley YZS-C10, Bacillus pumilus YZS-C11, and Bacillus aggregatus YZS-D08 are mixed in a mass ratio to prepare a solid mixed bacterial powder; a carrier is mixed with the solid mixed bacterial powder in a mass ratio of 100:2-5 to obtain a water treatment composite microbial preparation solid preparation; the carrier is bran and coral sand mixed in any proportion.

6. The water treatment composite microbial preparation according to claim 5, characterized in that: The fermentation broth of the Bacillus tequila YZS-C01, the Bacillus shafusus YZS-C03, the Bacillus valleculata YZS-C10, the Bacillus pumilus YZS-C11, and the Bacillus agglomerata YZS-D08 is mixed in a mass ratio of (1-3): (1-3): (1-3): (1-3): (2-5) to obtain the obtained product.

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