A biological promoter and its application in high-salt wastewater treatment

Abstract

The present invention discloses a bioaccelerator and its application in the biochemical treatment of high-salinity wastewater. The bioaccelerator comprises, by weight, 200-500 parts of potassium chloride, 100-200 parts of mineral-derived potassium fulvate, 100-200 parts of corn steep liquor powder, 100-200 parts of molasses powder, 50-200 parts of salt-tolerant yeast extract powder, and 0-200 parts of betaine. When applied to a high-salinity wastewater biochemical system with a salinity of 1% to 20%, the bioaccelerator can significantly improve the system's salt tolerance, resistance to salinity shock, pollutant degradation efficiency, and operational stability.

Description

Technical Field

[0001] The invention belongs to the technical field of wastewater biological treatment, and particularly relates to a biological promoter and application thereof in high-salt wastewater treatment. Background Art

[0002] Wastewater bioaccelerators are generally defined as substances that can increase the diversity of degrading microorganisms, improve the stress resistance of degrading microorganisms, and enhance the activity of degrading microorganisms, thereby improving the degradation efficiency of wastewater treatment pollutants, improving sewage treatment effects, and improving the system's ability to resist shock. In a high-salt wastewater environment, high salinity exerts a high osmotic pressure stress on microbial cells, leading to problems such as separation of the cell wall of ordinary microorganisms, obstruction of metabolic enzyme activity, decreased metabolic rate, and even cell death; toxic substances in high-salt industrial wastewater have a toxic inhibitory effect on cells. The unique osmotic pressure balance mechanism of salt-tolerant bacteria and halophilic bacteria, such as K absorption, + exclude + , produce or accumulate compatible solutes, synthesize sugars, amino acids, etc. to regulate the osmotic pressure balance inside and outside the cells, and can stabilize and protect the integrity of the bacterial cells and the activity of enzymes in the bacteria.

[0003] Chinese patent application 201210341306.4 discloses a compound osmoprotectant and its application. The osmoprotectant, composed of a powder mixture of betaine, trehalose, and potassium chloride in a mass ratio of 80-120:10-30:1, significantly enhances activated sludge's tolerance to high salinity concentrations and its pollutant degradation efficiency. At a NaCl concentration of up to 5%, the addition of the osmoprotectant increased COD degradation efficiency in the biochemical treatment system by 1.5 times, with effluent COD below 100 mg / L. The components used in this application are all osmoprotectants, intended to enhance salt tolerance but not to increase microbial diversity and activity. From a practical perspective, the formulation is unsuitable, expensive, and costly. Among the components, only potassium chloride is relatively inexpensive. While betaine and trehalose can be produced industrially, they still cost tens to hundreds of thousands of yuan per ton.

[0004] Chinese patent application 201510267375.9 discloses a method for using betaine as an additive to alleviate salinity shock in anaerobic ammonium oxidation systems. The anaerobic ammonium oxidation SBR reactor is used to treat saline wastewater, and the volatile suspended sludge concentration (VSS) is maintained at 6-9 g / L; the influent NH4 + The N concentration is 180-220 mg / L. By adding betaine to maintain the betaine concentration in the reactor at 0.1-5 mmol / L, the activity recovery time of the anaerobismic SBR reactor under 3% salinity influent shock conditions can be effectively shortened, and a higher total nitrogen removal rate can be achieved. This application only adds betaine, a single osmotic protectant, which has a more limited function and is relatively expensive. Fully dosing betaine would cost a ton of water.

[0005] Chinese patent application 201710290540.1 discloses an enhancer and method for treating high-salt, low-temperature wastewater. The enhancer is made by uniformly mixing mannitol, tetrahydropyrimidine, trehalose, amino acids, potassium salts, inositol methyl esters, fructan, dimethyl sulfoxide, methanol and acetamide. The enhancer works by adding 5 to 25 kg of the enhancer per ton of water to adjust the osmotic pressure of microbial cells, so that the microorganisms can play a role in treating high-salt wastewater.

[0006] Chinese patent application 201510110266.6 discloses a promoter for promoting the growth of salt-tolerant nitrifying bacteria in high-salinity wastewater. The promoter comprises: per 1000ml of water, 30-40g of sodium chloride, 0.2-0.6g of ammonium sulfate, 0.01-0.05g of magnesium sulfate heptahydrate, 0.8-1.5g of dipotassium hydrogen phosphate, 0.01-0.05g of ferrous sulfate heptahydrate, 7-8.2g of calcium chloride, 1-1.7g of sodium bicarbonate, 0-0.5g of sodium nitrite, 1-5ml of vitamin solution, 1-5ml of trace element solution, and 5-10ml of bacterial solution, with a pH of 7.8. The promoter in this application is actually a nutrient and has a complex composition. Furthermore, the promoter can only treat high-salinity wastewater with a concentration of 3%, and its effectiveness is limited for higher concentrations.

[0007] Chinese patent application 201910707346.8 discloses a microbial growth-promoting nutrient, its preparation method, and application. The nutrient composition is: 5-15% sodium humate, 1.4-1.8% mixed vitamins, 1-2% peptone, 0.5-1% yeast extract powder, 0.2-0.3% disodium adenosine triphosphate, and the remainder water. This application also discloses nutrients and is costly.

[0008] As can be seen from the above, the main components of existing high-salinity wastewater bioaccelerators include osmoprotectants, organic acids, polysaccharides, surfactants, amino acids, toxicity buffers, trace elements, and other ingredients to improve the treatment effect of high-salinity wastewater. However, the functions of these bioaccelerators have the following problems: ① Their functions are relatively simple or they focus on comprehensive nutrition, improving microbial salt tolerance, detoxification, or increasing activity; ② Their use costs are high: their components are complex or the production costs of the main components are high; ③ They are only effective for high-salinity wastewater with a salt content of no more than 3-5%. If they are used for high-salinity wastewater with a salt content of more than 8%, the cost of adding existing bioaccelerators will be very high. Summary of the Invention

[0009] In view of the problems of salinity inhibition, toxic inhibition of microbial activity and low efficiency of high-salt wastewater treatment, the present invention aims to provide a low-cost bioaccelerator for high-salt wastewater treatment and its application.

[0010] The technical solution adopted by the present invention to solve the technical problem is:

[0011] In a first aspect, the present invention provides a biological promoter for treating high-salt wastewater, which comprises, by weight, 200-500 parts of potassium chloride, 100-300 parts of mineral potassium fulvic acid, 100-200 parts of corn steep liquor powder, 100-200 parts of molasses powder, 50-100 parts of salt-tolerant yeast extract powder, and 0-100 parts of betaine.

[0012] Preferably, the salt-tolerant yeast extract powder is prepared by fermenting and culturing salt-tolerant yeast, concentrating, autolyzing, enzymolyzing, inactivating, solid-liquid separation, vacuum concentrating and spray drying.

[0013] The salt concentration of the salt-tolerant yeast fermentation culture is 10% to 15% NaCl.

[0014] Further preferably, the salt-tolerant yeast is selected from one or more of Candida, Rhodotorula, Rhodotorula sphaeroides, and Saccharomyces rouxii.

[0015] Further preferably, the Candida is Candida prachuapensi LH-Y.0003, which is deposited in the General Microbiology Center of the China Culture Collection Administration, with a deposit number of CGMCC NO.19111, and a deposit date of December 9, 2019; the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0016] Further preferably, the red yeast Rhodotorula mucilaginosa is red yeast Rhodotorula mucilaginosa LH-Y.0007, which is deposited in the General Microbiology Center of the China Culture Collection Administration, with a deposit number of CGMCC No. 19113; deposit date: December 9, 2019; deposit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0017] Further preferably, the Rhodosporidium sphaerocarpum is Rhodosporidium sphaerocarpum LH-Y.0008, which is deposited in the General Microbiology Center of the China Culture Collection Administration, with a deposit number of CGMCC No. 19114; deposit date: December 9, 2019; deposit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0018] More preferably, the salt-tolerant Saccharomyces rouxii is Saccharomyces rouxii for high-salt soy sauce fermentation.

[0019] Preferably, the components of the biological promoter include, by weight: 200-500 parts of potassium chloride, 150-200 parts of mineral potassium fulvic acid, 100-150 parts of corn steep liquor powder, 150-200 parts of molasses powder, 50-100 parts of salt-tolerant yeast extract powder, and 0-200 parts of betaine.

[0020] Preferably, the biological promoter comprises, by weight, 200 parts of potassium chloride, 200 parts of mineral-source potassium fulvic acid, 100 parts of corn steep liquor powder, 200 parts of molasses powder, 100 parts of salt-tolerant yeast extract powder, and 200 parts of betaine.

[0021] Preferably, the biological promoter comprises, by weight, 500 parts of potassium chloride, 200 parts of mineral-source potassium fulvic acid, 100 parts of corn steep liquor powder, 150 parts of molasses powder, 50 parts of salt-tolerant yeast extract powder, and 0 parts of betaine.

[0022] Preferably, the biological promoter comprises, by weight, 350 parts of potassium chloride, 150 parts of mineral potassium fulvic acid, 150 parts of corn steep liquor powder, 150 parts of molasses powder, 100 parts of salt-tolerant yeast extract powder, and 50 parts of betaine.

[0023] Further preferably, the above-mentioned bioaccelerator is prepared into a liquid, and its preparation method is to add it into water according to the mass ratio in the formula and stir until the total mass concentration after the components are dissolved is 10% to 20%, and the pH is adjusted to 3.0 to 4.0 with hydrochloric acid, and the salinity of the solution is adjusted to 5% to 15% by adding NaCl.

[0024] In a second aspect, the present invention provides a use of the above-mentioned biopromoter in the treatment of high-salt wastewater.

[0025] Optionally, the salinity of the high-salt wastewater is above 1%. Specifically, the salinity of the high-salt wastewater is 1-25%, such as 1%-3%, 4%-7%, 8%-12%, 13%-17% or 18%-25%.

[0026] Optionally, the inorganic salt in the high-salt wastewater is one or more of sodium chloride, sodium sulfate or calcium chloride.

[0027] Optionally, the high-salt wastewater has a biochemical process, and the biochemical process is anaerobic, anaerobic hydrolysis, hydrolysis acidification, microaerobic, aerobic, nitrification or denitrification.

[0028] Optionally, the high-salt wastewater biochemical process is an activated sludge process or a biofilm process; preferably, when the salt concentration of the wastewater exceeds 3%, the high-salt wastewater biochemical process is preferably a biofilm process, such as a suspended biofiller biofilm process, a hanging rope filler biofilm process and a fixed bed filter media biofilm process; further preferably, the high-salt wastewater biochemical process is a hanging rope filler biofilm using bio-rope filler.

[0029] Optionally, according to different salinity, water quality and process, the addition concentration of the biopromoter in high-salt wastewater treatment is 1-50 mg / L, which can be 1-5 mg / L, 5-20 mg / L, 20-100 mg / L, 100-200 mg / L or 200-500 mg / L, etc.

[0030] Optionally, the water temperature of the high-salt wastewater biochemical treatment pool is 10-50°C, such as 10-15°C, 15-38°C or 40-48°C; more preferably, the water temperature of the high-salt wastewater biochemical treatment pool is 22-35°C.

[0031] Optionally, the pH of the high-salt wastewater biochemical treatment pool is 3.0-5.0, 6.0-9.0 or 9.1-10.5; preferably, the pH of the high-salt wastewater biochemical treatment pool is 7.6-8.0, 8.1-8.3, 8.4-8.6 or 8.7-9.0.

[0032] Preferably, the high-salinity wastewater biochemical process is a biofilm method using suspended bio-rope fillers, and meets the following requirements:

[0033] (1) The water temperature of the high-salt wastewater biochemical treatment pool is 22-35°C;

[0034] (2) The pH of the high-salt wastewater biochemical treatment tank is 8.1-8.3, 8.4-8.6 or 8.7-9.0;

[0035] (3) The diameter of the bio-rope filler is 20-30 mm, and the installation spacing is 60 mm × 60 mm;

[0036] (4) The dissolved oxygen in the high-salt wastewater biochemical pool is 0.1-0.3 mg / L in the anoxic pool and 3.0-5.0 mg / L in the aerobic pool;

[0037] (5) The bioaccelerator is added in a multi-point dispersed manner.

[0038] Preferably, the bioaccelerator is used to treat high-salt wastewater in typical industries, and the method is as follows:

[0039] The bioaccelerator is used for high-salt wastewater produced by the chlorohydrin process of propylene oxide: the biochemical treatment process is A / O+secondary sedimentation tank+contact oxidation+coagulation sedimentation; the bioaccelerator is added to the aerobic tank and the primary contact oxidation tank, and the addition concentrations are 5-20 mg / L and 1-5 mg / L respectively.

[0040] The bioaccelerator is used for high-salt wastewater produced by the chlorohydrin process of propylene oxide, and the optimized treatment method is as follows: the biochemical treatment process is "A / O micro-oxygenation + secondary sedimentation tank + contact oxidation + coagulation sedimentation", wherein the hydraulic retention time (HRT) of the A / O micro-oxygenation tank is 6 to 12 hours, and the contact oxidation retention time (HRT) is 8 to 16 hours; the bioaccelerator is added to the A / O micro-oxygenation tank and the contact oxidation tank, and the addition concentrations are 10 to 20 mg / L and 2 to 10 mg / L, respectively.

[0041] The biological accelerator is used for organosilicon high-salt and high-calcium wastewater: the biochemical treatment process is "anaerobic hydrolysis + primary contact oxidation + ozone oxidation + secondary contact oxidation", wherein the anaerobic hydrolysis residence time is 16 to 24 hours, the primary contact oxidation residence time is 6 to 12 hours, the ozone oxidation residence time is 1 to 2 hours, and the secondary contact oxidation residence time is 2 to 4 hours; the biological accelerator is added to the anaerobic hydrolysis tank, and the addition concentration is 50 to 100 mg / L.

[0042] The bioaccelerator is used for sebacic acid production high-salt and high-sodium sulfate wastewater: the sodium sulfate content is 6-10%, and the biochemical treatment process is a "microaerobic aeration + MBR + primary contact oxidation + ozone oxidation + secondary contact oxidation" process, wherein the microaerobic aeration tank residence time is 20-60 hours, the MBR residence time is 2-4 hours, and the contact oxidation residence time is 12-24 hours; the bioaccelerator is added to the microaerobic aeration tank and the primary contact oxidation tank, and the addition concentrations are 20-50 mg / L and 2-10 mg / L, respectively.

[0043] The biological promoter is used for RO concentrated brine generated by membrane reuse of industrial wastewater biochemical tail water: the TDS is 10,000-40,000 mg / L, and the biochemical treatment process is "denitrification-pre-ozonation-nitrification-post-ozonation-post-biofilm" and is constructed in combination, wherein the denitrification residence time is 2-6 hours, the pre-ozonation residence time is 40-80 minutes, the nitrification residence time is 4-8 hours, the post-ozonation residence time is 30-60 minutes, the post-biofilm residence time is 2-4 hours, and the nitrified liquid is returned to the denitrification tank at a reflux ratio of 100-400%; the biological promoter is added in the denitrification tank, and the addition concentration is 1-5 mg / L.

[0044] The biological accelerator is used for phenol acetone production wastewater: TDS is 20,000-50,000 mg / L, COD is 3,000-10,000 mg / L, and its biochemical treatment process is "microaerobic hydrolysis + aerobic + MBR + contact oxidation + ozone + biofilm", wherein the microaerobic hydrolysis residence time is 24-48 hours, the aerobic tank residence time is 24-72 hours, the front contact oxidation residence time is 12-24 hours, the ozone oxidation residence time is 2-4 hours, and the rear biofilm residence time is 6-12 hours; the biological accelerator is added to the microaerobic hydrolysis tank, the aerobic tank, and the contact oxidation tank, and the addition concentrations are 20-50 mg / L, 10-20 mg / L, and 1-5 mg / L, respectively.

[0045] The bioaccelerator is used for lithium battery disassembly production wastewater: TDS is 30,000-150,000 mg / L, COD is 200-1,200 mg / L, and NH3-N is 40-200 mg / L. Its biochemical treatment process is "anoxic / aerobic + secondary sedimentation tank ① + ozone oxidation + contact oxidation + secondary sedimentation tank ②", and the contact oxidation effluent is returned to the front-end anoxic / aerobic tank with a reflux ratio of 100%-300%, wherein the anoxic / aerobic tank residence time is 12-60 hours, the ozone oxidation residence time is 2-4 hours, and the post-biofilm residence time is 6-18 hours; the bioaccelerator is added to the anoxic tank and the contact oxidation tank, and the addition concentrations are 5-20 mg / L and 2-10 mg / L, respectively.

[0046] The present invention also provides a method for treating high-salinity wastewater, which comprises adding the aforementioned bioaccelerator to the high-salinity wastewater.

[0047] The potassium fulvate used in the bioaccelerator of this invention is a product made by combining fulvic acid extracted or fermented from weathered coal, lignite, peat, plant straw, sawdust, bagasse, and kitchen waste with potassium hydroxide or other conditions. Potassium fulvate includes both mineral-derived potassium fulvate and biochemical potassium fulvate. Mineral-derived potassium fulvate is derived from mineral sources such as weathered coal, lignite, and peat, and can contain over 50% fulvic acid. Biochemical potassium fulvate is derived from plant straw, sawdust, bagasse, and kitchen waste, and can contain over 35% fulvic acid. Potassium fulvate can contain 8% to 12% or more of K2O, and also contains nitrogen, phosphorus, amino acids, vitamins, and trace elements. Potassium fulvate is primarily used in agriculture and soil improvement. It can be applied directly or as a foliar fertilizer, flushing fertilizer, organic fertilizer, pesticide fertilizer, micronutrient fertilizer, aquaculture fertilizer, liquid mulch film, humic acid water-retaining agent, or additive. It can also be used as a coating for slow-release fertilizers in high-tower spray granulation of organic and inorganic compound fertilizers. Potassium fulvate is a standard industrial product with high production volume and low price. Due to its abundant raw material resources, potassium fulvate can be produced on a large scale at low cost.

[0048] The corn steep liquor powder used in the bioaccelerator of the present invention is a byproduct of corn starch production. Its main components are amino acids and polypeptides, and it is also rich in vitamins and growth factors. It has a protein content exceeding 42%, a total nitrogen content of approximately 2.5%-4.5%, a total amino acid content of ≥35%, an ammonium nitrogen content of 0.3%-0.7%, a reducing sugar content of 1.2%-11%, a lactic acid content of 10%-15%, and a content of acetic acid and other organic acids of 0.1-0.3%. The ash content contains 10-20% inorganic elements such as K, Mg, Ca, Fe, P, and trace elements, of which approximately 1.5% is dissolved, based on dry matter. The total phosphorus content is above 1.0%. Corn steep liquor powder can be used as a microbial fermentation medium, replacing expensive organic nitrogen sources. As a byproduct of corn processing, corn steep liquor powder offers high yield and low cost.

[0049] The molasses powder used in the bioaccelerator is a type of biochemical potassium fulvic acid, made from sugarcane molasses concentrate. It contains nutrients such as nitrogen, phosphorus, potassium, various amino acids, enzymes, and polysaccharides. It contains ≥60% organic matter, ≥47% biochemical fulvic acid, ≥4% humic acid, ≥12% total nutrients, ≥3% free amino acids, and ≥5% chelated trace elements. Molasses powder is primarily used for agricultural soil improvement and as a microbial fermentation medium. As a byproduct of sucrose production, molasses powder offers high yields and low costs.

[0050] The yeast extract powder of the present invention is also called yeast extract. It is a biological culture medium product rich in nutrients such as protein, amino acids, peptides, polypeptides, nucleic acids, vitamins and trace elements, which is made from high-protein baker's yeast or brewer's yeast through processes such as autolysis, enzymolysis, concentration and drying.

[0051] The salt-tolerant yeast extract powder used in the bioaccelerator of the present invention is prepared from salt-tolerant yeast through fermentation, concentration, autolysis, enzymatic hydrolysis, inactivation, solid-liquid separation, vacuum concentration, and spray drying, among other processes. The salt-tolerant yeast is selected from one or more of the following: salt-tolerant Candida albicans, salt-tolerant Rhodotorula glutinosae, salt-tolerant Rhodotorula glutinosae, salt-tolerant Rhodotorula globosum, and salt-tolerant Rhodotorula rouxii. The salt-tolerant yeast can be fermented and cultured in 5% to 20% NaCl, and the types and concentrations of intracellular amino acids are significantly different from those of yeast grown under low-salt conditions. In particular, the salt-tolerant yeast contains osmoprotectants (also known as compatible solutes) related to microbial salt tolerance, such as trehalose, amino acids (such as glutamic acid and proline), amino acid derivatives (betaine and ectoine), and potassium ions.

[0052] The preferred yeasts for preparing the salt-tolerant biological accelerator of the present invention are Candida prachuapensi CGMCC No. 19111, Rhodotorula mucilaginosa CGMCC No. 19113, Rhodosporidium sphaerocarpum CGMCC No. 19114 and Saccharomyces rouxii, all of which can grow and reproduce in an environment with a salinity of more than 10% NaCl.

[0053] Betaine, also known as trimethylamine betaine or trimethylglycine, is chemically similar to methionine and choline. As an important osmoprotectant, betaine can regulate the osmotic pressure balance inside and outside cells. Betaine is chemically synthesized from trimethylamine and chloroacetic acid in an aqueous solution, followed by separation and purification by recrystallization. It is low-cost and can be produced on a large scale.

[0054] The various raw material components of the bioaccelerator of the present invention do not function alone, but rather interact with each other to produce a synergistic effect. For example, potassium chloride, potassium fulvic acid, corn steep liquor powder, molasses powder, and betaine can provide various compatible solute substances required by salt-tolerant microorganisms to regulate osmotic pressure, such as potassium ions, amino acids, polysaccharides, amino acid derivatives, etc., which together improve the microorganisms' salt tolerance and resistance to salinity shock. Potassium fulvic acid, corn steep liquor powder, and molasses powder can also provide rich nutrients such as N, P, K, trace elements, amino acids, vitamins, and life factors in addition to compatible solutes, which are directly utilized by microorganisms to increase the metabolic activity of microbial cells, thereby improving their salt tolerance and pollutant degradation capabilities. The various raw material components do not function alone, but rather interact with each other to produce a synergistic effect.

[0055] Beneficial effects

[0056] The present invention provides a bioaccelerator and its application in the treatment of high-salt wastewater, which has the following beneficial effects compared with the prior art:

[0057] (1) The biopromoter of the present invention fully considers the main factors affecting the salt tolerance, salinity shock resistance, and pollutant degradation ability of the biochemical treatment of high-salt wastewater. The selected components contain a rich variety of compatible solutes and a rich variety of nutrients. The biochemical treatment of high-salt wastewater is highly targeted and the formula is reasonable.

[0058] (2) The selected components of the biopromoter of the present invention can play a good combined and synergistic role, effectively improving the salt tolerance and metabolic activity of microbial cells in the high-salt wastewater biochemical system, thereby enhancing the biochemical system's ability to resist salinity shock and pollutant degradation.

[0059] (3) The components selected for the biopromoter of the present invention are all selected from industrial products that can be produced on a large scale, and the cost of each component is low; and by optimizing the component formula, the overall cost of the biopromoter is further reduced; and the cost of using the biopromoter for biological treatment of high-salt wastewater is effectively reduced.

[0060] (4) The biopromoter of the present invention is applicable to high-salinity wastewater with a salinity of 1% to 25%, and is particularly applicable to high-salinity wastewater with a salinity of 5% to 20%, with good effects and lower costs.

[0061] Preservation Instructions 1

[0062] Species name: Candida;

[0063] Latin name: Candida prachuapensis;

[0064] Strain ID: LH-Y.0003;

[0065] Depository: China General Microbial Culture Collection Center;

[0066] Abbreviation of depository institution: CGMCC;

[0067] Storage address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing;

[0068] Deposit date: December 9, 2019;

[0069] The registration number of the collection center is: CGMCC NO.19111.

[0070] Collection Instructions 2

[0071] Species name: Red yeast rice;

[0072] Latin name: Rhodotorula mucilaginosa;

[0073] Strain ID: LH-Y.0007;

[0074] Depository: China General Microbial Culture Collection Center;

[0075] Abbreviation of depository institution: CGMCC;

[0076] Storage address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing;

[0077] Deposit date: December 9, 2019;

[0078] The registration number of the collection center is: CGMCC NO.19113.

[0079] Collection Instructions 3

[0080] Species name: Rhodosporidium sphericalensis;

[0081] Latin name: Rhodosporidium sphaerocarpum;

[0082] Strain ID: LH-Y.0008;

[0083] Depository: China General Microbial Culture Collection Center;

[0084] Abbreviation of depository institution: CGMCC;

[0085] Storage address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing;

[0086] Deposit date: December 9, 2019;

[0087] The registration number of the collection center is: CGMCC NO.19114. DETAILED DESCRIPTION

[0088] The preparation and application of the accelerator of the present invention are described in detail below with reference to specific examples.

[0089] Example 1

[0090] A biological accelerator consists of the following components by weight: 500 parts of potassium chloride, 150 parts of mineral potassium fulvic acid, 100 parts of corn steep liquor dry powder, 150 parts of molasses dry powder, 50 parts of salt-tolerant yeast extract powder, and 50 parts of betaine.

[0091] The salt-tolerant yeast extract powder used is salt-tolerant Candida prachuapensi CGMCC NO.19111 and is prepared through fermentation culture, concentration, autolysis, enzymolysis, inactivation, solid-liquid separation, vacuum concentration, spray drying and other processes.

[0092] Example 2

[0093] A biological accelerator consists of the following components by weight: 200 parts of potassium chloride, 200 parts of mineral-source potassium fulvic acid, 100 parts of corn steep liquor dry powder, 200 parts of molasses dry powder, 100 parts of salt-tolerant yeast extract powder, and 200 parts of betaine.

[0094] The salt-tolerant yeast extract powder is prepared by fermenting and culturing salt-tolerant rouxigensis yeast at 15% NaCl salinity, concentrating, autolyzing, enzymolyzing, inactivating, separating solid from liquid, vacuum concentrating, spray drying and other processes.

[0095] The above-mentioned biological promoter is prepared into a liquid, and the pH value is adjusted to 3.0-4.0 with hydrochloric acid, wherein the mass concentration of the promoter effective component is 10%, and the mass concentration of NaCl is 10%.

[0096] Example 3

[0097] A biological accelerator consists of the following components by weight: 300 parts of potassium chloride, 100 parts of mineral-source potassium fulvic acid, 100 parts of corn steep liquor dry powder, 100 parts of molasses dry powder, 100 parts of salt-tolerant yeast extract powder, and 100 parts of betaine.

[0098] The salt-tolerant yeast extract powder is salt-tolerant Candida prachuapiensi CGMCC No. 19111, which is prepared through fermentation culture at 10% NaCl salinity, concentration, autolysis, enzymolysis, inactivation, solid-liquid separation, vacuum concentration, spray drying and other processes.

[0099] The above-mentioned biological promoter is prepared into a liquid, and the pH value is adjusted to 3.5-4.0 with hydrochloric acid, wherein the mass concentration of the active component of the promoter is 15%, and the mass concentration of NaCl is 10%.

[0100] Example 4

[0101] A biological accelerator consists of the following components by weight: 350 parts of potassium chloride, 150 parts of mineral potassium fulvic acid, 150 parts of corn steep liquor dry powder, 150 parts of molasses dry powder, 100 parts of salt-tolerant yeast extract powder, and 50 parts of betaine.

[0102] The salt-tolerant yeast extract powder is salt-tolerant Candida prachuapiensi CGMCC No. 19111, which is prepared through fermentation culture at 10% NaCl salinity, concentration, autolysis, enzymolysis, inactivation, solid-liquid separation, vacuum concentration, spray drying and other processes.

[0103] The above-mentioned biological promoter is prepared into a liquid, and the pH value is adjusted to 3.0-4.0 with hydrochloric acid, wherein the mass concentration of the promoter effective component is 10%, and the mass concentration of NaCl is 10%.

[0104] Example 5

[0105] A biological accelerator consists of the following components by weight: 400 parts of potassium chloride, 200 parts of mineral potassium fulvic acid, 200 parts of corn steep liquor dry powder, 1100 parts of molasses dry powder, 100 parts of salt-tolerant yeast extract powder, and 50 parts of betaine.

[0106] The salt-tolerant yeast extract powder is prepared from salt-tolerant Rhodosporidium sphaerocarpum CGMCC No. 19114 through fermentation culture at 10% NaCl salinity, concentration, autolysis, enzymolysis, inactivation, solid-liquid separation, vacuum concentration, spray drying and other processes.

[0107] The above-mentioned biological promoter is prepared into a liquid, and the pH value is adjusted to 3.0-4.0 with hydrochloric acid, wherein the mass concentration of the promoter effective component is 10%, and the mass concentration of NaCl is 10%.

[0108] Example 6

[0109] A bioaccelerator, comprising the following components by weight:

[0110] 400 parts of potassium chloride, 200 parts of mineral source potassium fulvic acid, 200 parts of corn steep liquor dry powder, 1100 parts of molasses dry powder, 100 parts of salt-tolerant yeast extract powder, and 50 parts of betaine.

[0111] The salt-tolerant yeast extract powder is salt-tolerant red yeast Rhodotorula mucilaginosa, with a preservation number of CGMCC No. 19113, and is prepared through fermentation culture at 5% NaCl salinity, concentration, autolysis, enzymolysis, inactivation, solid-liquid separation, vacuum concentration, spray drying and other processes.

[0112] The above-mentioned biological promoter is prepared into a liquid, and the pH value is adjusted to 3.0-4.0 with hydrochloric acid, wherein the mass concentration of the promoter effective component is 10%, and the mass concentration of NaCl is 10%.

[0113] Example 7

[0114] The bioaccelerators 1 to 6 described in Examples 1 to 6 were compounded or purchased with bioaccelerators A, B, C, and D to conduct the following experiments to test their performance.

[0115] Among them, biopromoter A was purchased from Mianjin Environmental Protection Technology (Shanghai) Co., Ltd., Bio Max-PromotionAgent, and its main components include: sodium chloride, ammonium sulfate, magnesium sulfate heptahydrate, potassium dihydrogen phosphate, ferrous sulfate, calcium chloride, sodium bicarbonate, sodium nitrite, vitamins, and trace elements.

[0116] Biological promoter B was purchased from Shenzhen Mingyueqing Environmental Biotechnology Co., Ltd., XL microbial promoter, the main components of which include yeast powder, peptone, ferrous ammonium sulfate, lysine, aspartic acid, serine, arginine, vitamin A, vitamin B, vitamin C, vitamin H, and trace metal elements.

[0117] Betaine C was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., anhydrous, with a content of 98%.

[0118] Biological accelerator D is self-prepared, and its main ingredients are 80 parts of betaine + 20 parts of trehalose + 1 part of potassium chloride.

[0119] Ten parallel activated sludge experiments were set up, including 6 experimental groups, 2 control groups and 2 blank control groups; the activated sludge was taken from landfill leachate, with an inoculation concentration of MLSS of 4000 mg / L, a sludge settling ratio SV30 of 78%, and a salt tolerance concentration of 0.5% to 0.8%; the two blank groups were a high-salt blank control group without adding biological promoters and a low-salt blank control group.

[0120] Test groups 1-6 and control groups A and B, each treated with a commercial bioaccelerator, were dosed with 50 mg / L of bioaccelerator. Using an SBR process, the effective tank volume was 2 L, the influent volume was 2 L, and the influent was landfill leachate with a COD of 5000 mg / L. NaCl was added to achieve a salinity of 5% NaCl, and the water temperature was maintained at 25-30°C. The average COD removal rates after continuous bioaccelerator addition for 3, 7, and 14 days are shown in Table 1.

[0121] Table 1 COD removal effect of high-salt wastewater with different bioaccelerators

[0122] 3 days 7 days 14 days <![CDATA[SV 30 / %]]> Low salt control (no accelerator) 48.3 70.4 85.2 78 High salt control (no accelerator) 27.5 48.4 34.2 73 Biological accelerator 1 45.2 68.3 86.1 52 Bioaccelerator 2 49.7 72.6 90.4 49 Biological accelerator 3 50.1 74.3 92.9 46 Bioaccelerator 4 49.7 75.2 93.4 45 Bioaccelerator 5 52.3 77.7 93.9 43 Bioaccelerator 6 54.1 80.9 95.1 40 Purchased biological accelerator A 28.4 41.6 46.7 63 Purchased biological accelerator B 45.1 63.5 76.3 66 Purchased biological accelerator C 34.3 50.4 65.2 79 Purchased biological accelerator D 33.5 48.3 63.8 80

[0123] It can be seen from Table 1 that after adding the biological promoter of the present invention:

[0124] The biochemical system quickly adapted to the 5% NaCl high-salt wastewater treatment system, and the COD removal rates at 3d, 7d, and 14d did not decrease. In fact, the COD removal rates of some experimental groups were higher than those of the low-salt control group.

[0125] The COD removal rate of the 5% NaCl high-salt control group without adding accelerator was significantly lower than that of the low-salt control group. Moreover, as the test time increased, the COD removal rate decreased after 14 days. This indicates that salt has a serious inhibitory effect on the microorganisms in the biochemical system and even causes cell decomposition.

[0126] Commercially available bioaccelerator A had no significant effect on improving the COD removal rate under 5% NaCl salinity conditions. Commercially available bioaccelerator B had an effect on improving the COD removal rate under 5% NaCl salinity conditions, but was inferior to that of the present invention. Commercially available bioaccelerators C and D had an effect on improving the COD removal rate under 5% NaCl salinity conditions, but their effects were inferior to those of the bioaccelerator of the present invention.

[0127] Example 8

[0128] Example 5 Biopromoter is used for the treatment of high-salt wastewater from propylene oxide produced by the chlorohydrin process, with a salinity of 4.2% NaCl. The wastewater biochemical treatment process is A / O+secondary settling tank+contact oxidation+coagulation sedimentation, and the residence times of A / O and contact oxidation tanks are 36h and 12h, respectively; A / O is an activated sludge process, with a sludge concentration of 20,000mg / L and a dissolved oxygen of 2.0-5.0mg / L; the first-level contact oxidation uses biological rope filler with an installation density of 60%, an installation spacing of 100mm×100mm, a filler diameter of 50mm, and a dissolved oxygen of 4.0-6.0mg / L.

[0129] The two treatment series of the above wastewater treatment process were set up for comparison between adding an accelerator and not adding an accelerator, and except for the addition of the biological accelerator, other process parameters were the same.

[0130] The above-mentioned bioaccelerators were added to the aerobic tank and the primary contact oxidation tank at concentrations of 10 mg / L and 5 mg / L, respectively; the addition was continuous; after 90 days, the water quality indicators of the inlet and outlet water of the sewage treatment system are shown in Table 2.

[0131] Table 2 Effect of the biopromoter of the present invention on the treatment of propylene oxide wastewater

[0132]

[0133] Example 9

[0134] Example 5 The biological promoter is used to treat high-salt wastewater produced by the chlorohydrin process of propylene oxide, with a salinity of 4.2% NaCl. The wastewater biochemical treatment process is microaerobic + secondary sedimentation tank + primary contact oxidation + ozone + secondary contact oxidation, wherein the HRT of the microaerobic hydrolysis tank is 10 h, the HRT of the primary contact oxidation is 12 h, and the HRT of the secondary contact oxidation is 6 h; the sludge concentration in the microaerobic tank is 25,000 mg / L, and the dissolved oxygen at the outlet is 0.1-0.5 mg / L; the primary contact oxidation uses biological rope filler with an installation density of 75%, an installation spacing of 60×60 mm, and a filler diameter of 20 mm, and the dissolved oxygen at the outlet is 4.0-6.0 mg / L.

[0135] The two wastewater treatment sequences mentioned above were set up for comparison between adding a promoter and not adding a promoter, and except for the addition of the biological promoter, other process parameters were the same.

[0136] The bioaccelerator was added to the microaerobic tank and the primary contact oxidation tank at concentrations of 20 mg / L and 5 mg / L, respectively. After 90 days, the influent and effluent quality indicators of the wastewater treatment system before and after the addition of the bioaccelerator are shown in Table 3.

[0137] Table 3 Effect of the biopromoter of the present invention on the treatment of propylene oxide wastewater

[0138]

[0139] Example 10

[0140] The biopromoter of Example 6 was used to treat organosilicon high-salt and high-calcium wastewater, the salinity of the wastewater was 2% to 3% CaCl2, and the wastewater biochemical treatment process was anaerobic hydrolysis + primary contact oxidation + ozone oxidation + secondary contact oxidation, wherein the anaerobic hydrolysis residence time was 18h, the primary contact oxidation residence time was 10h, the ozone oxidation residence time was 1h, and the secondary contact oxidation residence time was 3h; the anaerobic hydrolysis tank was installed with bio-rope filler, the installation density was 60%, the installation spacing was 100×100mm, the filler diameter was 50×50mm, the dissolved oxygen was <0.1mg / L, and the ORP was <-300mV; the primary contact oxidation tank was installed with bio-rope filler, the installation density was 75%, the installation spacing was 100×100mm, the filler diameter was 30×30mm, the dissolved oxygen was 3.0-6.0mg / L, and the pH was 7.0-9.0mg / L.

[0141] The two wastewater treatment sequences mentioned above were set up for comparison between adding a promoter and not adding a promoter, and except for the addition of the biological promoter, other process parameters were the same.

[0142] The bioaccelerator was added to the anaerobic hydrolysis tank at a concentration of 50 mg / L. The water quality indicators of the inlet and outlet water of the sewage treatment system with and without the bioaccelerator are shown in Table 4.

[0143] Table 4 Effect of the bioaccelerator of the present invention on the treatment of organosilicon wastewater

[0144]

[0145] Example 11

[0146] Example 4 The biological promoter is applied to the treatment of high-salt and high-sodium sulfate wastewater from sebacic acid production, with a sodium sulfate content of 6-10%. The wastewater biochemical treatment process is microaerobic aeration + MBR + primary contact oxidation + ozone oxidation + secondary contact oxidation, wherein the microaerobic aeration tank residence time is 48 hours, the MBR residence time is 3 hours, and the contact oxidation residence time is 15 hours; the microaerobic aeration tank adopts a mud film process, the biological filler is a biological rope filler, the filler installation density is 50%, the installation spacing is 150×150 mm, the filler diameter is 50×50 mm, the sludge concentration is 6000 mg / L, the dissolved oxygen is 0.3-1.0 mg / L, and the MBR membrane is selected from a flat membrane; the primary contact oxidation biological filler is a biological rope filler, the installation density is 60%, the installation spacing is 100×100 mm, the filler diameter is 30 mm, and the dissolved oxygen is 3.0-6.0 mg / L.

[0147] The two wastewater treatment sequences mentioned above were set up for comparison between adding a promoter and not adding a promoter, and except for the addition of the biological promoter, other process parameters were the same.

[0148] The bioaccelerator was added to the microaerobic aeration tank and the primary contact oxidation tank at concentrations of 50 mg / L and 10 mg / L, respectively. The water quality indicators of the inlet and outlet water of the sewage treatment system with and without the bioaccelerator are shown in Table 5.

[0149] Table 5 Effect of the biopromoter of the present invention on the treatment of sebacic acid wastewater

[0150]

[0151]

[0152] Example 12

[0153] Example 6 A bioaccelerator is used for RO concentrated brine treatment. The RO concentrated brine is produced by membrane reuse of industrial wastewater biochemical tail water, with a salinity of 2% to 4%. The wastewater biochemical treatment process is denitrification-pre-ozonation-nitrification-post-ozonation-post-biofilm, wherein the denitrification residence time is 5 hours, the pre-ozonation residence time is 1 hour, the nitrification residence time is 6 hours, the post-ozonation residence time is 50 minutes, and the post-biofilm residence time is 2 hours. The nitrified liquid is returned to the denitrification tank with a reflux ratio of 200%. The biochemical process adopts contact oxidation technology for both denitrification and nitrification. The packing installation density is 60%, the installation spacing is 100×100 mm, and the packing diameter is 20 mm. Sodium acetate or glucose is added to the denitrification tank as an external carbon source, and the dissolved oxygen is less than 0.3 mg / L. The dissolved oxygen in the nitrification tank is 3.0 to 5.0 mg / L.

[0154] The two wastewater treatment sequences mentioned above were set up for comparison between adding a promoter and not adding a promoter, and except for the addition of the biological promoter, other process parameters were the same.

[0155] The bioaccelerator was added to the denitrification tank at a concentration of 3 mg / L. The water quality indicators of the inlet and outlet water of the nitrification tank of the sewage treatment system with and without the bioaccelerator are shown in Table 6.

[0156] Table 6 Effect of the biopromoter of the present invention on the treatment of RO concentrated brine wastewater

[0157]

[0158] Example 13

[0159] Example 5: Biopromoter 10 was used to treat phenol-acetone production wastewater with a salinity of 3% to 4% and a COD of 3600 mg / L. The wastewater biochemical treatment process consisted of microaerobic hydrolysis + aerobic treatment + MBR + contact oxidation + ozone + biofilm treatment. The microaerobic hydrolysis retention time was 24 hours, the aerobic tank retention time was 48 hours, the pre-contact oxidation retention time was 18 hours, the ozone oxidation retention time was 2 hours, and the post-biofilm retention time was 7 hours. The biopromoter was added to the microaerobic hydrolysis tank, aerobic tank, and contact oxidation tank at concentrations of 20 mg / L, 12 mg / L, and 3 mg / L, respectively. The influent and effluent water quality indicators of the wastewater treatment system with and without the biopromoter are shown in Table 7.

[0160] Table 7 Effect of the biopromoter of the present invention on the treatment of phenol-acetone wastewater

[0161]

[0162]

[0163] Example 14

[0164] Example 3 Bioaccelerator is applied to the treatment of lithium battery disassembly production wastewater, wherein the lithium battery disassembly production wastewater has a salinity of 3% to 15%, a COD of 200 to 1200 mg / L, and an NH3-N of 40 to 200 mg / L. The wastewater biochemical treatment process is anoxic / aerobic + secondary sedimentation tank ① + ozone oxidation + contact oxidation + secondary sedimentation tank ②, wherein the anoxic / aerobic tank residence time is 48 hours, the ozone oxidation residence time is 3 hours, and the post-biofilm residence time is 1 hour. The anoxic / aerobic pool adopts biofilm technology and selects bio-rope filler with a filler installation density of 60%, an installation spacing of 100×100mm and a filler diameter of 30×30mm. The retention time of the anoxic pool is 16h, and the retention time of the aerobic pool is 32h. Glucose or sodium acetate is added to the anoxic pool as an additional carbon source for denitrification to make the dissolved oxygen <0.5mg / L. The aerobic pool adopts cyclone aeration and the dissolved oxygen is 3.0-6.0mg / L.

[0165] The two wastewater treatment sequences mentioned above were set up for comparison between adding a promoter and not adding a promoter, and except for the addition of the biological promoter, other process parameters were the same.

[0166] The bioaccelerator was added to the anoxic tank and the contact oxidation tank at concentrations of 10 mg / L and 5 mg / L, respectively. The water quality indicators of the inlet and outlet water of the sewage treatment system with and without the bioaccelerator are shown in Table 8.

[0167] Table 8 Effect of the bio-promoter of the present invention on the treatment of lithium battery disassembly wastewater

[0168]

[0169] The applicant studied the technical solution of the present invention and found that the accelerator of Example 2 is used in high-salinity wastewater with a salinity of 18% to 25%, the accelerator of Example 3 is used in high-salinity wastewater with a salinity of 13% to 17%, the accelerator of Example 4 is used in high-salinity wastewater with a salinity of 8% to 12%, the accelerator of Example 5 is used in high-salinity wastewater with a salinity of 3% to 7%, and the accelerator of Example 6 is used in high-salinity wastewater with a salinity of 1% to 3%, and the effect is better.

[0170] Example 15

[0171] The high-salt and high-calcium wastewater from propylene oxide produced by the glycerol process has a salinity of 4.2% NaCl, a COD of 578 mg / L, an NH3-N of 18 mg / L, and a TN of 29 mg / L. Two wastewater treatment plants:

[0172] The wastewater biochemical treatment process for Unit A consisted of an A / O reactor, a secondary sedimentation tank, a contact oxidation tank, and coagulation sedimentation. The A / O and contact oxidation tank residence times were 36 hours and 12 hours, respectively. The aerobic tank employed an activated sludge process, with a sludge concentration of 20,000 mg / L, a dissolved oxygen level of 2.0 to 5.0 mg / L, and sodium bicarbonate added to maintain a pH of 7.6 to 8.0. The water temperature was 30 to 32°C. The contact oxidation tank employed biological 6-rope packing with an installation density of 60%, an installation spacing of 100 mm x 100 mm, and a packing diameter of 50 mm. The dissolved oxygen level was 4.0 to 6.0 mg / L, and the water temperature was 30 to 32°C. Salt-tolerant nitrifying bacteria were simultaneously added to the A / O and contact oxidation tanks at a concentration of 2 mg / L. The biological promoter from Example 5 was added to the A / O and contact oxidation tanks at concentrations of 10 mg / L and 5 mg / L, respectively.

[0173] The biochemical treatment process of wastewater in device B is A / O+secondary sedimentation tank+contact oxidation tank+coagulation sedimentation. The residence time of A / O and contact oxidation tanks are 6+18h and 6h, respectively. Both A / O tank and contact oxidation tank use biological rope fillers with an installation density of 60%, an installation spacing of 60mm×60mm and a filler diameter of 25mm. Before the start of the experiment, activated sludge from propylene oxide wastewater was inoculated for biofilm formation with an inoculation concentration of 20,000mg / L. The biofilm was acclimated with propylene oxide wastewater for 7 days. Sodium bicarbonate was added to both A / O tank and contact oxidation tank to maintain the pH at 8.1-8.3. Both A / O tanks were aerated, and the dissolved oxygen was 0.1-0.3mg / L in the anoxic tank and 3.0-5.0mg / L in the aerobic tank. The dissolved oxygen in the contact oxidation tank was 4.0-6.0mg / L. The water temperature of both A / O tank and contact oxidation tank was 30-32℃. Salt-tolerant nitrifying bacteria were added to both the A / O and contact oxidation pools at a concentration of 2 mg / L. The bioaccelerator of Example 5 was added to both the A / O and contact oxidation pools, with concentrations of 5 mg / L, 5 mg / L, and 5 mg / L, respectively, to the A / O anoxic, aerobic, and contact oxidation pools.

[0174] After the A and B devices were operated continuously for 90 days, the inlet and outlet water quality indicators of the sewage treatment system were shown in Tables 9 and 10.

[0175] Table 9 The biopromoter of the present invention is applied to the COD treatment effect of propylene oxide wastewater

[0176]

[0177] Table 10 The effect of the biopromoter of the present invention on the treatment of NH3-N in propylene oxide wastewater

[0178]

[0179] Tables 9 and 10 show that both Units A and B utilize an "A / O + secondary sedimentation tank + contact oxidation tank + coagulation sedimentation" process, with a bioaccelerator dosage of 10 mg / L for the A / O tank and 5 mg / L for the contact oxidation tank. After adding the bioaccelerator, COD and NH3-N levels in the effluents of both Units A and B were significantly reduced. In Unit B, the A / O biochemical tanks utilize a biofilm process and are equipped with bio-rope packing with a diameter of 20 to 30 mm and a spacing of 60 mm x 60 mm. The anoxic tanks maintain a concentration of 0.1 to 0.3 mg / L, while the aerobic tanks maintain a concentration of 3.0 to 5.0 mg / L. Furthermore, the A / O tank bioaccelerator is dosed at multiple, dispersed locations. Consequently, COD and NH3-N levels in the effluents of Unit B are significantly lower.

[0180] Therefore, when adding the biopromoter of the present invention to high-salt wastewater, appropriate wastewater treatment process and process parameters should also be selected.

[0181] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field can easily modify the technical solutions recorded in the aforementioned embodiments within the technical scope disclosed in the present invention, or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A bioaccelerator, characterized in that: Contains the following components by mass ratio: 200-500 parts of potassium chloride, 100-300 parts of mineral-source potassium fulvic acid, 100-300 parts of corn steep liquor powder, 100-300 parts of molasses powder, 50-200 parts of salt-tolerant yeast extract powder, and 0-200 parts of betaine; The salt-tolerant yeast extract powder is prepared by fermenting, culturing, concentrating, autolyzing, enzymolyzing, inactivating, separating solid from liquid, vacuum concentrating and spray drying salt-tolerant yeast; The salt-tolerant yeast is selected from one or more of salt-tolerant Candida, salt-tolerant Rhodotorula, and salt-tolerant Rhodotorula; Wherein, the salt-tolerant Candida ( Candida prachuapensis ) LH-Y.0003, deposited in China General Microbiological Culture Collection, with the deposit number CGMCC NO.19111; The salt-tolerant red yeast ( Rhodotorula mucilaginosa ) LH-Y.0007, deposited in China General Microbiological Culture Collection, with the deposit number CGMCC No.19113; The halotolerant Rhodosporidium sphericalensis ( Rhodosporidium sphaerocarpum )LH-Y.0008 ， It is deposited in China General Microorganism Culture Collection with the deposit number CGMCC No.19114.

2. A biopromoter according to claim 1, characterized in that: The composition of the biopromoter is selected from any one of (A1) to (A3) by weight: (A1) 200 parts of potassium chloride, 200 parts of mineral-source potassium fulvic acid, 100 parts of corn steep liquor powder, 200 parts of molasses powder, 100 parts of salt-tolerant yeast extract powder, and 200 parts of betaine; (A2) 500 parts of potassium chloride, 200 parts of potassium fulvic acid, 100 parts of corn steep liquor powder, 150 parts of molasses powder, 50 parts of salt-tolerant yeast extract powder, and 0 parts of betaine; (A3) 350 parts of potassium chloride, 150 parts of potassium fulvic acid, 150 parts of corn steep liquor powder, 150 parts of molasses powder, 100 parts of salt-tolerant yeast extract powder, and 50 parts of betaine.

3. A biopromoter according to any one of claims 1 to 2, characterized in that: The bioaccelerator is prepared into a liquid by adding the liquid into water according to the mass ratio in the formula and stirring to dissolve the components to a total mass concentration of 10% to 20%, adjusting the pH to 3.0 to 4.0 with hydrochloric acid, and adding NaCl to adjust the salinity of the solution to 5% to 15%.

4. Use of a biopromoter according to any one of claims 1 to 3 in the treatment of high-salt wastewater.

5. The use of a biopromoter in the treatment of high-salt wastewater according to claim 4, characterized in that: The salinity of the high-salt wastewater is 1-25%.

6. The use according to claim 5, characterized in that The salinity of the high-salt wastewater is 1% to 3%, 4% to 7%, 8% to 12%, 13% to 17% or 18% to 25%.

7. The use according to claim 5, characterized in that The inorganic salts in the high-salt wastewater are mainly sodium chloride, sodium sulfate, calcium chloride or mixed salts.

8. The use of a bioaccelerator in high-salt wastewater treatment according to claim 4, characterized in that: The addition concentration of the biological promoter is 1-500 mg / L.

9. Use of a biopromoter in high-salt wastewater treatment according to claim 4, characterized in that: The addition concentration of the biological promoter is 1-5 mg / L, 5-20 mg / L, 20-100 mg / L, 100-200 mg / L or 200-500 mg / L.

10. Use of a bioaccelerator in high-salt wastewater treatment according to claim 4, characterized in that: The high-salinity wastewater treatment has a biochemical process, and the biochemical process is anaerobic, anaerobic hydrolysis, hydrolysis acidification, microaerobic, aerobic, nitrification, heterotrophic denitrification, autotrophic denitrification or sulfur oxidation.

11. Use of a bioaccelerator in high-salt wastewater treatment according to claim 4, characterized in that: The biochemical process for high-salt wastewater is the activated sludge process or the biofilm process.

12. Use of a bioaccelerator in high-salt wastewater treatment according to claim 11, characterized in that: The biochemical process for high-salinity wastewater is a biofilm method using suspended bio-rope fillers and meets the following requirements: (1) The water temperature of the high-salinity wastewater biochemical treatment pool is 22~35℃; (2) The pH of the high-salinity wastewater biochemical treatment tank is 8.1-8.3, 8.4-8.6 or 8.7-9.0; (3) The diameter of the bio-rope filler is 20~30mm, and the installation spacing is 60mm×60mm; (4) The dissolved oxygen in the biochemical pool of high-salt wastewater is 0.1~0.3 mg / L in the anoxic pool and 3.0~5.0 mg / L in the aerobic pool; (5) The bioaccelerator is added in a multi-point and dispersed manner.

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