A settling and cleaning device for an integrated settling tank

Abstract

The application relates to a settling and cleaning device of an integrated settling tank. The application is characterized in that the inner cavity of the used integrated settling tank is cleaned efficiently by the participation of the rotating settling disc device, the cleaning column device and the stirring paddle device, and in combination with the unique and efficient cleaning liquid; the upper end surface of the cleaning column on the cleaning column device is provided with a negative pressure cleaning flow channel for discharging the liquid and cleaning the bottom of the integrated settling tank, so that the residual contaminated cells on the inner wall of the integrated settling tank can be effectively removed, and the purpose of repeatedly using the integrated settling tank is achieved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of biological equipment, and particularly relates to a settling and cleaning device of an integrated settling tank. BACKGROUND

[0002] With people paying more and more attention to diseases, accurate diagnosis of the body condition becomes a decisive basis for measuring the health status of the body, and therefore, pathological tissue examination is very important for disease confirmation and treatment. Therefore, pathological cell examination needs to be performed through preparation of a slice, and the slice preparation methods include smearing, printing, and settling. The settling method includes natural settling and centrifugal settling. The natural settling method is to place a solution containing cells in a container, and the cells in the solution settle to the bottom of the solution under the action of gravity. After the cells are settled, the supernatant is poured or sucked off, and the cells on the bottom of the container are the cell slice. The centrifugal settling method is to place a solution containing cells in a container, and then place the container in a centrifuge to accelerate the cells in the container to the bottom of the container under the action of centrifugal force. After the cells are settled, the supernatant is poured or sucked off to obtain the cell slice.

[0003] After searching, the patent document 202011075096.X discloses a settling tank cleaning device, which comprises a settling cup, a settling cup base, a cleaning plate, and a cleaning plate groove part. The settling cup is fixed in the settling cup base through the ear handles on both sides of the settling cup, and the opening part of the settling cup is higher than the settling cup base. The cleaning plate is arranged at any position around the outer circumference of the settling tank. The cleaning plate groove part is arranged on the back of the cleaning plate, and is used for cleaning and draining the settling tank. However, the device cannot effectively clean the inner wall of the settling tank, and the bottom of the settling tank cannot be flushed. In addition, the bottom of the settling tank of the device is coated with glue, and there is a gap between the coated glue and the PP material. Cells can hide in the gap, and the cleaning liquid cannot flush and remove the hidden cells, which may cause cross contamination.

[0004] The stirring paddle device of the present application can effectively flush and clean the inner wall of the integrated settling tank, and remove cell residues and dirt. The cleaning column device of the present application optimizes the shortcomings of the cleaning plate in the previous patent. The previous cleaning plate only has the function of spitting and sucking cleaning liquid, and cannot effectively flush the bottom of the integrated settling tank, and cannot prevent cell residues. The upper end face of the existing cleaning column device is designed with a negative pressure cleaning flow channel, and the whole negative pressure cleaning flow channel is designed to be very narrow, so that the cleaning liquid can be rapidly flushed along the knife surface of the integrated settling tank. Then, the cleaning with anhydrous ethanol can prevent cell residues from remaining in the inner wall of the integrated settling tank, and can also make the integrated settling tank dry quickly. In addition, the settling tank of the present application is an integrated settling tank, and does not have any dead volume or space for cell hiding. In addition, the surface is smooth and anti-adhesion, which is beneficial to the full flushing and cleaning of the stirring paddle device and the cleaning column device. SUMMARY

[0005] The present application aims at the problems in the prior art, and provides a settling and cleaning device for an integrated settling chamber. The device can effectively clean the pollution cells remaining on the inner wall of the integrated settling chamber by rotating the settling disc device, the cleaning column device and the stirring paddle device, and combining with the unique and efficient cleaning liquid, so as to achieve the purpose of reusing the integrated settling chamber.

[0006] The object of the present application can be achieved by the following scheme:

[0007] The present application provides a settling and cleaning device for an integrated settling chamber, which comprises a rotating settling disc device, a cleaning column device, a stirring paddle device and an integrated settling chamber.

[0008] The circumferential position of the rotating settling disc device is uniformly provided with a plurality of chamber seats for fixing the integrated settling chamber. The cleaning column device is provided with a plurality of cleaning columns, and the upper end surface of the cleaning column is provided with a negative pressure cleaning flow channel for cleaning the bottom of the integrated settling chamber. The stirring paddle device is provided with a plurality of stirring paddles for cleaning the inner cavity of the integrated settling chamber. During cleaning, the stirring paddle suspended above the integrated settling chamber is lowered into the inner cavity of the integrated settling chamber for rotating stirring, and the cleaning column located below the settling chamber is raised to abut against the bottom flange surface of the integrated settling chamber to clean the bottom of the integrated settling chamber.

[0009] As an embodiment of the present application, the bottom sealing blade structure of the integrated settling chamber adopts a circular arc R angle or a circular ring plane, and both the sealing blade structures can form a smooth sealing surface. The R angle is 0.05-2mm, the outer diameter of the circular ring plane is larger than the inner diameter of the circular ring plane by 0.05-3mm, and the inner diameter of the circular ring plane is 10-14mm. The height of the sealing blade is 0.1-5mm. The material of the integrated settling chamber includes one of engineering plastics (PP, UPE, PE, PTFE) and ceramics. The sealing blade can tightly adhere to the glass slide to achieve the sealing effect. The bottom of the integrated settling chamber is provided with a flange, and the sealing blade of the bottom protrudes from the bottom surface of the flange of the settling chamber.

[0010] As an embodiment of the present application, the integrated settling chamber is pressed on the glass slide by the pressure of the compression spring of 10-100N, and the sealing blade is slightly deformed under the action of the pressure, so as to more effectively adhere to the upper surface of the glass slide to form a circular sealing ring, which can effectively block the leakage of liquid. The compression spring is located on the settling disc and has a downward pressure on the chamber seat, and the settling chamber is fixed on the chamber seat and presses the glass slide downward, so that the sealing blade of the settling chamber tightly adheres to the glass slide.

[0011] As an embodiment of the present application, the negative pressure cleaning flow path of the cleaning column upper end surface comprises, from inside to outside, a liquid blocking ring, a liquid flushing groove, an outer ring vacuum groove, and a leak-proof sealing ring. The liquid blocking ring is provided with a plurality of notches to form flushing groove inlets. The flushing groove inlets are 0.2-2 mm wide, 0.1-1 mm deep, and 2-8 in number, preferably 4, evenly distributed along the circumference and alternately distributed with the liquid blocking ring.

[0012] As an embodiment of the present application, the cleaning column upper end surface is further provided with a cleaning liquid outlet, an anhydrous ethanol outlet, and a vacuum suction port. The cleaning liquid outlet and the anhydrous ethanol outlet are located inside the leak-proof sealing ring, and the number thereof is preferably 1. The vacuum suction port is located outside the liquid flushing groove and is evenly distributed in the circumferential direction, and the number thereof is 2-8, preferably 4.

[0013] The outer ring vacuum groove draws anhydrous ethanol through the vacuum suction port. For cleaning the anhydrous ethanol remaining on the periphery of the integrated settling bin sealing blade, the cleaning column periphery is provided with an outer ring vacuum groove. Under the action of a strong vacuum system, air passes through three narrow semi-closed spaces (flushing groove inlets, liquid flushing grooves, and outer ring vacuum grooves) to form a narrow and urgent negative pressure space, forcing the anhydrous ethanol remaining on the periphery of the integrated settling bin sealing blade to enter the vacuum suction port, and finally cleaning the anhydrous ethanol on the periphery of the integrated settling bin sealing blade.

[0014] As an embodiment of the present application, the cleaning column can move up and down. During cleaning, the cleaning column rises, the cleaning column upper end surface lifts the settling bin bottom surface, and the settling bin reaches the cleaning position. The cleaning column upper end surface does not fit the sealing blade, and the gap between the liquid blocking ring and the sealing blade is 0.05-0.5 mm. The leak-proof sealing ring fits the lower surface of the integrated settling bin flange, and presses against the integrated settling bin flange to form a seal. The sealing blade is located directly above the liquid blocking ring, and the purpose is to block as much liquid as possible from entering the liquid flushing groove from the blocking ring, so that most of the liquid enters the liquid flushing groove from the liquid flushing groove inlets, and in the narrow channel of the liquid flushing groove, the liquid flushes the integrated settling bin blade in the circumferential direction. Compared with a simple cleaning plate provided with an inlet and a suction port, the cleaning column of the present application is designed with a complex and effective cleaning flushing structure, and the cleaning liquid and anhydrous ethanol outlets clean more thoroughly.

[0015] As an embodiment of the present application, the cleaning liquid outlet is connected to a cleaning liquid supply system, the anhydrous ethanol outlet is connected to an anhydrous ethanol supply system, and the cleaning column upper end surface is provided with a plurality of evenly distributed vacuum suction ports and is connected to a vacuum negative pressure system. A cleaning column Z-axis motor is provided below the cleaning column to enable the cleaning column to move up and down. The number of cleaning columns is preferably 1-3, and more preferably 2.

[0016] As an embodiment of the present application, the paddle head of the stirring paddle is provided with several layers of stirring blades, preferably 4 layers, each layer having 1-10 stirring blades, preferably 3; the stirring blades can be cubic or any columnar; the stirring paddle is 1-3, more preferably 2, which can simultaneously rotate and stir and clean the inner cavities of multiple integrated settling tanks; the surface of the stirring paddle is sprayed with a nano-hydrophobic coating, Teflon or fluorine-based plastic, which maximizes the anti-adhesion performance of the surface of the stirring paddle. The stirring paddle is powered by a stirring paddle Z-axis motor to realize up-and-down operation, and is powered by a stirring paddle motor to realize high-speed rotation.

[0017] The present application also provides a cleaning method for the cleaning device of the integrated settling tank, which comprises the following steps:

[0018] Step 1: Install the integrated settling tank on the tank seat of the rotatable settling disc device; under the action of the compression spring, the knife surface is attached to the upper surface of the glass slide to form a circular sealing ring;

[0019] Step 2: After the exfoliated cells of different individuals are settled and dyed in the integrated settling tank, the glass slide is automatically separated from the rotating settling disc; the integrated settling tank contaminated by cells is transferred to the cleaning position one by one; the stirring paddle on the stirring paddle device descends into the inner cavity of the integrated settling tank, and the stirring paddle rotates at high speed;

[0020] Step 3: The cleaning column on the cleaning column device rises and presses against the bottom surface of the integrated settling tank; the cleaning liquid outlet on the cleaning column discharges cleaning liquid into the cavity of the integrated settling tank;

[0021] Step 4: After the stirring paddle stirs the cleaning liquid, the vacuum negative pressure liquid suction port on the cleaning column sucks out all the cleaning liquid in the integrated settling tank;

[0022] Step 5: Repeat steps 3 and 4 several times;

[0023] Step 6: The anhydrous ethanol outlet on the cleaning column discharges anhydrous ethanol into the cavity of the integrated settling tank;

[0024] Step 7: After the stirring paddle stirs the anhydrous ethanol, the vacuum negative pressure liquid suction port on the cleaning column sucks out all the anhydrous ethanol in the integrated settling tank;

[0025] Step 8: The stirring paddle stops rotating and rises to the original position, and the cleaning column descends to the original position, and the settling tank cleaning is completed.

[0026] As an embodiment of the present application, in the first step, the rotatable settling disc device, the circumferential position of the rotating settling disc device, is uniformly distributed with a plurality of, preferably 20, pockets, which can fix 20 integrated settling pockets; in the process of cleaning the integrated settling pocket, the rotating settling disc device is driven by the large disc motor to make the integrated settling pocket pass through the stirring paddle device and the cleaning column device, so that the settling pockets can be cleaned one by one. The material of the integrated settling pocket can be any engineering plastic or ceramic, the bottom sealing blade structure adopts a circular arc R angle, the R angle is 0.05-2mm; or the sealing blade structure adopts a circular ring plane, the inner diameter of the circular ring plane is 10-14mm, the outer diameter of the circular ring is larger than the inner diameter of the circular ring by 0.05-3mm; the sealing blade height is 0.1-5mm; both kinds of sealing blades can form a smooth sealing surface.

[0027] As an embodiment of the present application, in the second step, the stirring paddle device, a plurality of stirring paddles are fixed on a Z axis, preferably two stirring paddles, can simultaneously rotate and stir and clean the inner cavities of two integrated settling pockets; the surface of the stirring paddle is sprayed with a nano hydrophobic coating, Teflon or fluorine plastic, which maximizes the anti-adhesion performance of the surface of the stirring paddle; the paddle head of the stirring paddle is provided with four layers of stirring blades, three stirring blades in each layer, which are uniformly distributed, the stirring blades can be cubic or any columnar; the stirring paddle is powered by a stirring paddle Z axis motor to realize up and down operation, and is powered by a stirring paddle motor to realize high-speed rotation, the rotation speed is 60-1000 rad / min, after the stirring paddle rotates, it can form a rapid vortex water flow to wash and clean the inner wall and bottom of the settling pocket, which can effectively clean all cell residues and prevent cross contamination. The cleaning position is that the pocket seat is turned to the upper side of the cleaning column, and the stirring paddle is directly below, ready to clean the settling pocket.

[0028] As an embodiment of the present application, in the third step, the mutual sticking force between the cleaning column of the cleaning column device and the bottom surface of the settling pocket is 10-100N; a cleaning column Z axis motor drives two cleaning columns to operate up and down, after rising, the upper end surface of the cleaning column lifts the bottom surface of the settling pocket, so that the settling pocket reaches the cleaning position, the cleaning liquid outlet on the upper end surface of the cleaning column discharges 1-3ml of cleaning liquid into the cavity of the integrated settling pocket. The cleaning liquid can make the cells / impurities more easily separate from the inner wall of the settling pocket and remove the staining liquid residue. The cleaning liquid is a hyperbranched bubble-free cleaning liquid.

[0029] The cleaning fluid outlet is connected to the cleaning fluid supply system, and the anhydrous ethanol outlet is connected to the anhydrous ethanol supply system. Four evenly distributed vacuum suction ports are provided on the upper surface of the cleaning column and connected to a vacuum negative pressure system. The four liquid blocking rings reduce the gap between the cleaning column and the bottom of the settling chamber (0.05-0.5mm), reducing the amount of cleaning fluid flowing into the liquid flushing tank from above the liquid blocking rings. This allows most of the cleaning fluid to flow rapidly into the four flushing tanks from their inlets, ensuring that the majority of the cleaning fluid flushes along the circumferential direction of the integrated settling chamber's blade surface. In the narrow cleaning space, this improves the flushing efficiency and force, more effectively cleaning the sealing blade surface of the integrated settling chamber. Finally, the cleaning fluid is drained through the vacuum suction ports. The leak-proof sealing ring ensures that the liquid injected into the integrated settling chamber does not overflow.

[0030] In one embodiment of the present invention, in the fourth step, the stirring time of the stirring paddle is 3-30s; in the fifth step, the third and fourth steps are repeated 2-5 times; in the sixth step, the amount of anhydrous ethanol discharged from the anhydrous ethanol outlet on the cleaning column is 1-3ml.

[0031] In one embodiment of the present invention, in the seventh step, anhydrous ethanol is extracted through the vacuum suction port. For cleaning the anhydrous ethanol remaining on the outer periphery of the sealing blade of the integrated settling chamber, an outer vacuum groove is provided around the cleaning column. Under the action of a powerful vacuum system, air passes through three narrow semi-enclosed spaces (flushing groove inlet, liquid flushing groove, and outer vacuum groove), forming a rapid and narrow negative pressure space, which forces the anhydrous ethanol remaining on the outer periphery of the sealing blade of the integrated settling chamber into the vacuum suction port, and finally cleans the anhydrous ethanol on the outer periphery of the sealing blade of the integrated settling chamber.

[0032] This invention also provides an integrated settling chamber. The sealing blade structure at the bottom of the integrated settling chamber adopts either a rounded radius (R-angle) or a circular annular plane. Both types of sealing blades can form a smooth sealing surface. When the sealing blade is pressed onto the glass slide, it undergoes slight deformation under pressure, thereby more effectively adhering to the upper surface of the glass slide and forming a circular sealing ring, which can effectively prevent liquid leakage. The radius (R-angle) is 0.05-2 mm. The outer diameter of the circular annular plane is 0.05-3 mm larger than the inner diameter, and the inner diameter of the circular annular plane is 10-14 mm. The height of the sealing blade is 0.1-5 mm. The integrated settling chamber has a flange at the bottom, and the sealing blade protrudes from the bottom surface of the flange.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The integrated settling chamber of this invention adopts a unique sealing blade structure and hard contact with the glass slide, realizing the hard sealing function of the integrated settling chamber. This solves the problem that traditional disposable settling chambers must rely on rubber and glass slides for sealing: the bond between rubber and plastic has gaps and is not strong; rubber has low density, large material pores, and is sticky; these characteristics make it difficult to remove cells after they adhere to it, and incomplete removal will bring the risk of cross-contamination. The integrated settling chamber of this invention is made entirely of engineering plastic, with no gaps or dead corners, a smooth surface, and is easy to clean.

[0035] (2) This invention utilizes a rotating settling plate device, a cleaning column device, and a stirring paddle device, combined with a unique and highly efficient cleaning fluid, to efficiently clean the inner cavity of a used integrated settling chamber. This effectively and completely removes residual contaminant cells from the inner cavity of the integrated settling chamber, making it reusable and solving the problem of traditional disposable settling chambers not being reusable. The cleaning fluid washes away dirt and cells from the surface of the integrated settling chamber, and ethanol replaces the cleaning fluid, reducing the concentration of the cleaning fluid on the integrated settling chamber to an extremely low level. Additionally, it allows for rapid dehydration and drying of the surface of the integrated settling chamber. Attached Figure Description

[0036] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0037] Figure 1 For cleaning devices;

[0038] Figure 2 Schematic diagram of the rotating settling plate at different angles;

[0039] Figure 3 A schematic diagram showing the state of the integrated settling chamber pressed against the glass slide;

[0040] Figure 4 A schematic diagram of an integrated settling chamber;

[0041] Figure 5 A detailed cross-sectional diagram of the sealing blade of an integrated settling chamber;

[0042] Figure 6 A schematic diagram showing the integrated settling chamber and the glass slide in place.

[0043] Figure 7 This is a schematic diagram of the stirring paddle device;

[0044] Figure 8 This is a schematic diagram of the agitator.

[0045] Figure 9 This is a schematic diagram of the cleaning column device;

[0046] Figure 10 A schematic diagram showing the characteristics of the negative pressure cleaning flow channel on the upper end face of the cleaning column;

[0047] Figure 11 A cross-sectional view showing the flange faces of the cleaning column and the integrated settling chamber in contact with each other.

[0048] In the picture:

[0049]

[0050] Detailed Implementation

[0051] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. The following examples are implemented under the premise of the technical solution of the present invention, providing detailed implementation methods and specific operating procedures, which will help those skilled in the art to further understand the present invention. It should be noted that the scope of protection of the present invention is not limited to the following embodiments; any adjustments and improvements made under the concept of the present invention are all within the scope of protection of the present invention.

[0052] Example 1

[0053] This invention provides a settling and cleaning device for an integrated settling chamber, such as... Figure 1 As shown, the cleaning device includes a rotating settling plate device 1, a cleaning column device 3, and an agitator device 2; it also includes a large disc motor 12. During cleaning, the rotating settling plate device 1, driven by the large disc motor 12, causes the integrated settling chamber 4 to pass through the agitator device 3 and the cleaning column device 2;

[0054] like Figure 2 As shown, 20 chamber seats 5 are evenly distributed around the circumference of the rotating settling plate device 1 to fix the integrated settling chamber 4; the state of the integrated settling chamber 4 on the glass slide 6 is as follows. Figure 3 , Figure 6 As shown, the integrated settling chamber 4 uses a pressure of 10-100N generated by the compression spring 7 mounted on the settling plate 27 to press the sealing blade 10 onto the glass slide 6. Under the pressure, the sealing blade 10 undergoes slight deformation, thus more effectively adhering to the upper surface of the glass slide 6 to form a circular sealing ring, which can effectively block liquid leakage.

[0055] like Figure 4 As shown, the integrated settling chamber 4 has a flange at the bottom, and the cross-section of the sealing blade 10 of the integrated settling chamber 4 is as follows. Figure 5 As shown, the bottom sealing blade 10 protrudes from the bottom surface 9 of the settling chamber flange. The bottom sealing blade 10 has a rounded R-angle 11 with a radius of 0.2 mm and a height of 3 mm. The integrated settling chamber 4 is made of PP.

[0056] The cleaning column device 3 is as follows Figure 9 As shown, there are two cleaning columns 17. The upper surface of the cleaning column 17 is provided with a negative pressure cleaning channel for liquid discharge and cleaning the bottom of the integrated settling tank 4.

[0057] The negative pressure cleaning channel is as follows Figure 10 As shown, from the inside out, the system includes a liquid blocking ring 21, a liquid flushing groove 20, an outer vacuum groove 26, and a leak-proof sealing ring 25. The liquid blocking ring 21 has four notches, forming flushing groove inlets 19. Each flushing groove inlet 19 is 0.5mm wide and 0.5mm deep, and there are four inlets evenly distributed around the circumference. The cleaning fluid outlet 22 and the anhydrous ethanol outlet 23 are located inside the leak-proof sealing ring 25, with one outlet each. Four vacuum suction ports 24 are located outside the liquid flushing groove 20, evenly distributed around the circumference. The cleaning column 17 can move up and down, and during cleaning... Figure 11 As shown, the cleaning column 17 rises, and its upper end face lifts the bottom surface of the integrated settling tank 4, so that the integrated settling tank 4 reaches the cleaning position; the upper end surface of the cleaning column 17 does not fit with the sealing blade 10, and the gap between the liquid blocking ring 21 and the sealing blade 10 is 0.2mm; the leak-proof sealing ring 25 fits with the bottom surface 9 of the settling tank flange, and presses against the flange of the integrated settling tank 4 to form a seal.

[0058] The cleaning fluid outlet 22 is connected to the cleaning fluid supply system, and the cleaning fluid is a hyperbranched, non-foaming cleaning fluid. The anhydrous ethanol outlet 23 is connected to the anhydrous ethanol supply system. The upper surface of the cleaning column 17 is provided with four evenly distributed vacuum suction ports 24, which are connected to a vacuum negative pressure system. A cleaning column Z-axis motor 18 is provided below the cleaning column 17, which can make the cleaning column 17 move up and down.

[0059] The stirring paddle device 2 is as follows Figure 7 As shown, two agitators 14 are used to clean the inner cavity 8 of the integrated settling chamber 4. The agitator heads are as follows: Figure 8 As shown, the device has four layers of stirring blades, with three stirring blades 16 in each layer, and the stirring blades 16 in each layer are evenly distributed. The stirring blades 16 are cuboid. The surface of the stirring paddle 14 is coated with Teflon to maximize its anti-adhesion properties. The stirring paddle is powered by a stirring paddle Z-axis motor 15 to move up and down, and by a stirring paddle motor 13 to rotate at high speed.

[0060] Example 2

[0061] The present invention provides a cleaning device for an integrated settling tank. The cleaning device includes a rotating settling disc device 1, a cleaning column device 3, and a stirring paddle device 2. During cleaning, the rotating settling disc device 1, driven by a large disc motor 12, causes the integrated settling tank 4 to pass through the stirring paddle device 3 and the cleaning column device 2.

[0062] The rotating settling plate device 1 has 20 bin seats 5 evenly distributed around its circumference for fixing the integrated settling bin 4; the cleaning column device 3 has two cleaning columns 17, and the upper surface of the cleaning column 17 has a negative pressure cleaning channel for liquid discharge and cleaning the bottom of the integrated settling bin 4; the stirring paddle device 2 has two stirring paddles 14 for cleaning the inner cavity 8 of the integrated settling bin 4.

[0063] The bottom sealing blade 10 of the integrated settling chamber 4 adopts a circular annular plane structure; the outer diameter of the circular annular plane is 0.2 mm larger than the inner diameter, and the inner diameter of the circular annular plane is 13 mm. The integrated settling chamber is made of ceramic.

[0064] The integrated settling chamber 4 uses a pressure of 10-100N generated by the compression spring 7 mounted on the settling plate 27 to press the sealing blade 10 onto the glass slide 6. Under the pressure, the sealing blade 10 undergoes slight deformation, thus more effectively adhering to the upper surface of the glass slide 6 to form a circular sealing ring, which can effectively block liquid leakage.

[0065] The negative pressure cleaning channel includes, from the inside out, a liquid blocking ring 21, a liquid flushing groove 20, an outer vacuum groove 26, and a leak-proof sealing ring 25. The liquid blocking ring 21 has four notches to form flushing groove inlets 19. The flushing groove inlets 19 are 0.5 mm wide and 0.5 mm deep, and there are 4 inlets, which are evenly distributed around the circumference. The cleaning liquid outlet 22 and the anhydrous ethanol outlet 23 are located inside the leak-proof sealing ring 25, and there is 1 outlet for each. The vacuum suction port 24 is located outside the liquid flushing groove 20, and there are 4 inlets evenly distributed around the circumference. The cleaning column 17 can move up and down. During cleaning, the cleaning column 17 rises, and its upper end face lifts the bottom surface of the integrated settling tank 4, so that the integrated settling tank 4 reaches the cleaning position. The upper end surface of the cleaning column 17 does not fit with the sealing blade 10, and the gap between the liquid blocking ring 21 and the sealing blade 10 is 0.2mm. The leak-proof sealing ring 25 fits with the bottom surface 9 of the settling tank flange and presses against the flange of the integrated settling tank 4 to form a seal.

[0066] The cleaning fluid outlet 22 is connected to the cleaning fluid supply system, and the cleaning fluid is a hyperbranched, non-foaming cleaning fluid. The anhydrous ethanol outlet 23 is connected to the anhydrous ethanol supply system. The upper surface of the cleaning column 17 is provided with several evenly distributed vacuum suction ports 24, which are connected to a vacuum negative pressure system. A cleaning column Z-axis motor 18 is provided below the cleaning column 17, which can make the cleaning column 17 move up and down.

[0067] The impeller head has four layers of stirring blades, with six stirring blades 16 in each layer, and the stirring blades 16 in each layer are evenly distributed; the stirring blades 16 are cuboid; the surface of the impeller 14 is coated with Teflon to maximize the anti-adhesion performance of the impeller 14 surface. The impeller is powered by the Z-axis motor 15 to move up and down, and by the motor 13 to rotate at high speed.

[0068] Example 3

[0069] This embodiment also provides a cleaning method using the cleaning device of the integrated settling tank described in Embodiment 1, the cleaning method comprising the following steps:

[0070] Step 1: Install the integrated settling chamber onto the chamber base of the rotatable settling disc device. Under the action of the compression spring, the glass slide adheres to the sealing blade surface, forming a circular sealing ring. The rotatable settling disc device has 20 chamber bases evenly distributed around its circumference, capable of fixing 20 integrated settling chambers. During the cleaning process, the rotatable settling disc device, driven by a large disc motor, moves the integrated settling chambers through the stirring paddle device and the cleaning column device, cleaning each chamber individually. The integrated settling chamber can be made of any engineering plastic or ceramic, and the bottom sealing blade surface structure adopts a rounded R-angle with an R-angle of 0.2mm; the sealing blade surface forms a smooth sealing surface.

[0071] Step 2: Exfoliated cells from different individuals settle and stain one by one in the integrated sedimentation chamber, then automatically detach from the rotating sedimentation plate. Cell-contaminated integrated sedimentation chambers are then moved to the cleaning position. The agitator on the stirring device descends into the inner cavity of the integrated sedimentation chamber while simultaneously rotating at high speed. The stirring device has two agitators fixed on one Z-axis, allowing simultaneous rotational stirring and cleaning of the inner cavities of two integrated sedimentation chambers. The agitator surface is coated with a nano-hydrophobic coating, Teflon, or fluoroplastics to maximize its anti-adhesion properties. The agitator head has four layers of stirring blades, three blades per layer, evenly distributed, and the blades are cuboid. The agitator is powered by a Z-axis motor for vertical movement and high-speed rotation (100 rad / min). The rotating agitator creates a rapid vortex of water that washes and cleans the inner walls and bottom of the sedimentation chamber, effectively removing all cell residue and preventing cross-contamination.

[0072] Step 3: The cleaning column on the cleaning column device rises and presses against the bottom surface of the integrated settling chamber flange; 2ml of cleaning liquid is discharged from the cleaning liquid outlet on the cleaning column into the integrated settling chamber cavity. In the cleaning column device, one cleaning column Z-axis motor drives two cleaning columns to move up and down. After rising, the upper end of the cleaning column pushes against the bottom surface of the settling chamber flange, bringing the settling chamber to the cleaning position. At this point, the force of the cleaning column and the settling chamber in contact is 60N.

[0073] The cleaning fluid outlet is connected to the cleaning fluid supply system, and the cleaning fluid is a hyperbranched, non-foaming cleaning fluid. The anhydrous ethanol outlet is connected to the anhydrous ethanol supply system. The upper surface of the cleaning column is provided with four evenly distributed vacuum suction ports, which are connected to a vacuum negative pressure system. The four liquid blocking rings reduce the gap between the cleaning column and the bottom of the settling chamber (0.2mm), which can reduce the flow of cleaning fluid from above the liquid blocking rings into the liquid flushing tank. This allows most of the cleaning fluid to flow rapidly into the four liquid flushing tanks from the four flushing tank inlets, so that the vast majority of the cleaning fluid flushes along the circumferential direction of the integrated settling tank blade. In the narrow cleaning space, this improves the flushing efficiency and flushing force of the cleaning fluid, and more effectively cleans the sealing blade of the integrated settling tank. Finally, the cleaning fluid is drained through the vacuum suction port. The leak-proof sealing ring ensures that the liquid injected into the integrated settling tank cavity does not overflow.

[0074] Step 4: After the agitator stirs the cleaning fluid for 20 seconds, the vacuum negative pressure suction port on the cleaning column will drain all the cleaning fluid from the integrated settling chamber.

[0075] Step 5: Repeat steps 3 and 4 three times.

[0076] Step 6: 2ml of anhydrous ethanol is dispensed from the anhydrous ethanol outlet on the cleaning column into the integrated settling chamber.

[0077] Step 7: After the agitator stirs the cleaning solution for 20 seconds, the vacuum negative pressure suction port on the cleaning column removes all the anhydrous ethanol from the integrated settling chamber. For the removal of anhydrous ethanol remaining on the outer edge of the sealing blade of the integrated settling chamber, an outer vacuum groove is provided around the cleaning column. Under the action of a powerful vacuum system, air passes through three narrow semi-enclosed spaces (flushing groove inlet, liquid flushing groove, and outer vacuum groove), forming a rapid and narrow negative pressure space. This forces the anhydrous ethanol remaining on the outer edge of the sealing blade of the integrated settling chamber into the vacuum suction port, ultimately removing all the anhydrous ethanol from the outer edge of the sealing blade of the integrated settling chamber.

[0078] Step 8: The agitator stops rotating and rises to the original position, the cleaning column descends to the original position, and the settling chamber cleaning is completed.

[0079] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various modifications or variations within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. A settling and cleaning device for an integrated settling chamber, characterized in that, The settling and cleaning device includes a rotating settling plate device, a cleaning column device, a stirring paddle device, and an integrated settling chamber. The rotating settling plate device has several compartment seats evenly distributed around its circumference for fixing the integrated settling tank; the cleaning column device has several cleaning columns, and the upper surface of the cleaning columns has a negative pressure cleaning channel for cleaning the bottom of the integrated settling tank; the stirring paddle device has multiple stirring paddles for cleaning the inner cavity of the integrated settling tank; during cleaning, the stirring paddle suspended directly above the integrated settling tank descends into the inner cavity of the integrated settling tank to rotate and stir, while the cleaning column located directly below the settling tank rises and presses against the bottom flange surface of the integrated settling tank to clean the bottom of the integrated settling tank; The negative pressure cleaning channel on the upper surface of the cleaning column includes, from the inside to the outside, a liquid blocking ring, a liquid flushing groove, an outer vacuum groove, and a leak-proof sealing ring; the liquid blocking ring has several notches to form the inlet of the flushing groove. The upper surface of the cleaning column is also provided with a cleaning fluid outlet, an anhydrous ethanol outlet, and a vacuum suction port; the cleaning fluid outlet and the anhydrous ethanol outlet are located inside the leak-proof sealing ring, and the vacuum suction port is located outside the liquid flushing tank. The cleaning fluid outlet is connected to the cleaning fluid supply system, the anhydrous ethanol outlet is connected to the anhydrous ethanol supply system, and the vacuum suction port is connected to the vacuum negative pressure system. The cleaning method of the settling and cleaning device of the integrated settling chamber includes the following steps: Step 1: Install the integrated settling chamber on the chamber base of the rotatable settling plate device. Under the action of the compression spring, the blade of the integrated settling chamber adheres to the upper surface of the glass slide, forming a circular sealing ring. Step 2: After the exfoliated cells from different individuals settle and are stained one by one in the integrated sedimentation chamber, the glass slides are automatically detached from the rotating sedimentation plate; the integrated sedimentation chambers contaminated with cells will be moved to the cleaning position one by one; the agitator on the stirring device descends into the inner cavity of the integrated sedimentation chamber, and at the same time the agitator rotates at high speed. Step 3: The cleaning column on the cleaning column device rises and presses against the bottom of the integrated settling tank; the cleaning liquid outlet on the cleaning column discharges the cleaning liquid into the inner cavity of the integrated settling tank. Step 4: After the agitator stirs the cleaning solution, the vacuum negative pressure suction port on the cleaning column draws out all the cleaning solution in the integrated settling chamber. Step 5: Repeat steps 3 and 4 several times; Step 6: Anhydrous ethanol is discharged from the anhydrous ethanol outlet on the cleaning column into the integrated settling chamber. Step 7: After stirring the anhydrous ethanol with the agitator, clean the vacuum negative pressure suction port on the column and drain all the anhydrous ethanol from the integrated settling chamber. Step 8: The agitator stops rotating and rises to the original position, the cleaning column descends to the original position, and the settling chamber cleaning is completed.

2. The sedimentation and cleaning device according to claim 1, characterized in that, The bottom sealing blade structure of the integrated settling chamber adopts either a rounded R-angle or a circular annular plane structure, both of which can form a smooth sealing surface.

3. The sedimentation and cleaning device according to claim 2, characterized in that, The integrated settling chamber uses a compression spring to press the sealing blade onto the glass slide. Under pressure, the sealing blade undergoes slight deformation, thus more effectively adhering to the surface of the glass slide and forming a circular sealing ring, which can effectively prevent liquid leakage.

4. The sedimentation and cleaning device according to claim 1, characterized in that, The cleaning column can move up and down. During cleaning, the cleaning column rises, and the upper end of the cleaning column lifts the bottom of the settling tank, so that the settling tank reaches the cleaning position. The upper end surface of the cleaning column does not fit with the sealing blade. The gap between the liquid blocking ring and the sealing blade is 0.05-0.5 mm. The leak-proof sealing ring fits with the lower surface of the integrated settling tank flange, pressing against the integrated settling tank flange to form a seal.

5. The sedimentation and cleaning device according to claim 1, characterized in that, The impeller head is provided with several layers of stirring blades, each layer having 1-10 stirring blades, and the stirring blades in each layer are evenly distributed.

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