A zebrafish larvae sampling device

Abstract

The present application relates to the technical field of biological sampling, and discloses a zebrafish larva sampling device, which comprises a handle, an external movable sleeve provided at the tail end of the handle, a fishing block provided on the movable sleeve, a fishing opening provided on the fishing block, two connecting blocks movably provided on the handle, a guide block fixed to the bottom of the fishing block, a receiving cavity provided in the handle, a plurality of openings provided on one side of the handle close to the fishing opening and communicated with the receiving cavity, a closing block hinged to the inner wall of the top of the opening and used for closing the opening, a sampling box slidingly arranged in the receiving cavity below the opening, and a supporting frame fixed to the inner wall of the receiving cavity and arranged at the bottom of the sampling box. The present application can quickly sample zebrafish larvae, and can adaptively regulate the sampling device according to sampling requirements. Moreover, the sampling device can be flexibly switched between manual and non-manual operation, has high working performance, and is convenient to use.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological sampling, in particular to a zebrafish fry sampling device. BACKGROUND

[0002] In production and scientific research work, it is often necessary to sample zebrafish fry in cultivation in order for staff to monitor the growth of the zebrafish fry.

[0003] The existing sampling method for zebrafish fry is mostly to use a net to fish out the zebrafish fry in a cultivation tank, then put the zebrafish fry in the net into a corresponding sampling box, and then repeat the above work until the sample is sufficient. This sampling method can sample zebrafish fry, but the process is tedious, and the staff can easily harm the zebrafish fry in the process of transporting the zebrafish fry back and forth, so we propose a zebrafish cultivation tank. SUMMARY

[0004] To solve the technical problem of tedious sampling steps of zebrafish fry, the present application provides a zebrafish fry sampling device.

[0005] The present application adopts the following technical solution: a zebrafish fry sampling device, comprising a handle, an external movable sleeve of the tail end of the handle is provided with a fishing block, a fishing opening is formed in the fishing block, two sides of the fishing block are provided with connecting blocks movably sleeved on the outside of the handle, a guide block is further fixed to the bottom of the fishing block, the handle is provided with a receiving cavity, a plurality of openings are formed in one side of the handle close to the fishing opening and in communication with the receiving cavity, an inner wall of the top of the opening is hinged with a closing block for closing the opening, a sampling box is slidably arranged in the receiving cavity below the opening, and a support frame is fixed to the inner wall of the receiving cavity at the bottom of the sampling box. Through the cooperation of the fishing block, the fishing opening, the guide block, the opening, the closing block and the sampling box, the zebrafish fry can be quickly sampled and collected.

[0006] As a further improvement of the above-mentioned solution, the driving sleeve is embedded at one end away from the fishing mouth, the inside of the driving sleeve is threaded with a screw rod, one end of the screw rod is rotatably connected to the connecting block at the tail end of the handle, the outside of the other end of the screw rod is movably sleeved with a stabilizing block sliding on the outside of the handle, a motor one is fixed to the connecting block at the top end of the handle, the output end of the motor one is drivingly connected with a transmission rod, the end of the transmission rod away from the motor one is provided with a sliding groove, the inside of the sliding groove is slidingly connected with a sliding block, the end of the screw rod away from the connecting block is provided with a slot, the end of the sliding block away from the transmission rod extends out of the inside of the sliding groove and slides in the inside of the slot, the sliding block outside the sliding groove and the slot is fixed with a toggle block, through the cooperation of the motor one, the transmission rod, the sliding block and the slot, the screw rod can be driven to rotate, the driving sleeve can be driven to vertically displace, the fishing block can be driven to vertically displace, by pulling the toggle block, the toggle block can be driven to vertically displace, the sliding block can be driven to vertically displace, when the sliding block is separated from the inside of the slot, the screw rod can be manually toggled to drive the screw rod to rotate.

[0007] As a further improvement of the above-mentioned solution, the inside wall of the receiving cavity away from the opening side is provided with a loading groove, the inside wall of the top of the loading groove is fixed with a motor two, the output end of the motor two is drivingly connected with a threaded rod, the other end of the threaded rod is rotatably connected to the inside wall of the bottom end of the loading groove, the outer wall of the threaded rod is threadedly sleeved with a plurality of threaded sleeves corresponding to the opening, the outer wall of the threaded sleeve is hingedly connected with a driving plate hingedly connected to the inside of the adjacent closing block, through the operation of the motor two, the threaded rod can be driven to rotate, the threaded sleeve can be driven to vertically displace, the driving plate can be driven to deflect, the closing block can be driven to deflect, the opening can be opened or closed.

[0008] As a further improvement of the above-mentioned solution, the sliding block is a rectangular block, the sliding groove and the slot are rectangular grooves, through the rectangular structure of the sliding block, the sliding groove and the slot, the sliding block can be driven to rotate when the transmission rod rotates, the screw rod can be driven to rotate.

[0009] As a further improvement of the above-mentioned solution, the outside of the motor one is sleeved with a protective shell one fixed to the adjacent connecting block, the outside of the motor two is sleeved with a protective shell two fixed to the inside wall of the loading groove, through the protective shell one, the motor one can be protected, through the protective shell two, the motor two can be protected.

[0010] As a further improvement of the above-mentioned solution, the outer wall of the screw rod in the inside of the stabilizing block is fixed with a plurality of limiting blocks, the inside wall of the stabilizing block is provided with an annular groove, the limiting blocks slide in the inside of the annular groove, through the cooperation of the limiting blocks and the annular groove, the screw rod can be limited to avoid deviation of the screw rod.

[0011] As a further improvement of the above-mentioned scheme, the connecting block is provided with a through hole, a positioning block is slidably arranged in the through hole, the outer side of the handle is provided with a positioning groove corresponding to the connecting block, one end of the positioning block is slidably arranged in the adjacent positioning groove, and the other end of the positioning block is fixedly provided with a handle, a plurality of return springs are fixed to the other end of the handle and fixed to the connecting block, the positioning block is pulled to drive the positioning block to be separated from the inside of the positioning groove, the connecting block is pulled to drive the connecting block, the fishing block and the stabilizing block to vertically displace, and the sampling assembly can be disassembled.

[0012] As a further improvement of the above-mentioned scheme, the outer side of the handle is hingedly provided with a movable door in communication with the receiving cavity, and the outer side of the movable door is provided with a sealing ring, the lofting box in the receiving cavity can be taken out by opening the movable door, and the sealing ring can increase the sealing performance of the movable door.

[0013] As a further improvement of the above-mentioned scheme, the outer side of the handle is hingedly provided with a movable door in communication with the receiving cavity, and the outer side of the movable door is provided with a sealing ring, the lofting box in the receiving cavity can be taken out by opening the movable door, and the sealing ring can increase the sealing performance of the movable door.

[0014] As a further improvement of the above-mentioned scheme, the width of the handle is greater than the width of the guide block, the outer side of the top of the handle is provided with anti-skid lines, and the outer side of the lofting box is attached with a label, the handle can be conveniently held by the user through the anti-skid lines, and the lofting box can be identified through the label.

[0015] Compared with the prior art, the beneficial effects of the present application are that:

[0016] The present application can quickly sample zebrafish larvae, avoid tedious fishing and sampling, has high sampling efficiency, can avoid harming zebrafish larvae in the process of back and forth transportation of zebrafish larvae, has high safety performance, and can adaptively regulate the sampling equipment according to the use demand of the user, and has high working performance.

[0017] The present application can manually and non-manually switch the sampling equipment according to the sampling demand of the staff, so as to facilitate the staff to sample zebrafish larvae, facilitate operation, and can simply and conveniently disassemble the sampling assembly of the sampling equipment. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a structural schematic view of a zebrafish larvae sampling device.

[0019] Figure 2 It is Figure 1 It is an enlarged structural schematic view of A in the middle.

[0020] Figure 3 It is Figure 1Structure schematic view of the enlarged portion at B;

[0021] Figure 4 For Figure 1 Structure schematic view of the enlarged portion at C;

[0022] Figure 5 For Figure 4 Structure schematic view of the enlarged portion at D;

[0023] Figure 6 Structure schematic view of the fishing block of a zebrafish fry sampling device;

[0024] Figure 7 Schematic view of a zebrafish fry sampling device.

[0025] Main symbol explanation:

[0026] Handle; 2, fishing block; 3, fishing mouth; 4, guide block; 5, storage cavity; 6, opening; 7, closing block; 8, driving plate; 9, loading groove; 10, threaded rod; 11, threaded sleeve; 12, return spring; 13, driving sleeve; 14, screw rod; 15, connecting block; 16, stabilizing block; 17, transmission rod; 18, sliding groove; 19, sliding block; 20, positioning block; 21, positioning groove; 22, movable door. DETAILED DESCRIPTION

[0027] In the following, the application will be further described in conjunction with the drawings and specific embodiments, and it should be noted that the following described embodiments or technical features can be combined in any manner to form new embodiments without conflict.

[0028] Embodiment 1

[0029] In combination with Figure 1 And Figure 3 , the zebrafish fry sampling device of the embodiment comprises a handle 1, an external movable sleeve at the tail end of the handle 1 is provided with a fishing block 2, the fishing block 2 is provided with a fishing mouth 3, both sides of the fishing block 2 are provided with connecting blocks 15 movably sleeved outside the handle 1, the bottom of the fishing block 2 is further provided with a guide block 4, the handle 1 is provided with a storage cavity 5, one side of the handle 1 close to the fishing mouth 3 is provided with a plurality of openings 6 in communication with the storage cavity 5, the inner wall of the top of the opening 6 is hinged with a closing block 7 for closing the opening 6, a sampling box is slidably arranged in the storage cavity 5 below the opening 6, and the bottom of the sampling box is provided with a support frame fixed to the inner wall of the storage cavity 5.

[0030] The implementation principle of the zebrafish juvenile sampling device in the embodiment of the application is as follows: when zebrafish juveniles need to be sampled, the handle 1 can be held by hand, the handle 1 is placed into the breeding device, at this time, the fishing mouth 3 on the fishing block 2 can be aimed at the zebrafish juveniles to be fished, the zebrafish juveniles are fished into the fishing mouth 3, when the zebrafish juveniles pass through the fishing mouth 3 and enter the inside of the guide block 4, at this time, the fishing block 2 performs vertical displacement, the guide block 4 performs vertical upward displacement, and the zebrafish juveniles in the inside of the guide block 4 perform vertical upward displacement, when the guide block 4 corresponds to the opening 6, at this time, the closing block 7 performs deflection, when the closing block 7 opens the opening 6, at this time, the zebrafish juveniles in the inside of the guide block 4 will enter the inside of the storage cavity 5 through the opening 6, fall into the corresponding sampling box, at this time, the closing block 7 performs deflection, when the closing block 7 closes the opening 6, at this time, the above work is repeated until the zebrafish juvenile sampling is completed.

[0031] Embodiment 2

[0032] In combination with Figure 2 and Figure 4 , the embodiment further improves the embodiment 1, and the improvement lies in that the end of the fishing block 2 away from the fishing mouth 3 is embedded with a driving sleeve 13, the inside of the driving sleeve 13 is threaded with a screw rod 14, one end of the screw rod 14 is rotationally connected to a connecting block 15 at the tail end of the handle 1, the other end of the screw rod 14 is externally movably sleeved with a stabilizing block 16 sliding on the outside of the handle 1, a motor one is fixed to the connecting block 15 at the top end of the handle 1, the motor one is a forward and reverse stepping motor, the output end of the motor one is transmissionally connected with a transmission rod 17, the end of the transmission rod 17 away from the motor one is provided with a sliding groove 18, the inside of the sliding groove 18 is slidingly connected with a sliding block 19, the end of the screw rod 14 away from the connecting block 15 is provided with a slot, the end of the sliding block 19 away from the transmission rod 17 extends out of the inside of the sliding groove 18 and slides in the inside of the slot, the sliding block 19 outside the sliding groove 18 and the slot is fixed with a poking block, when the fishing block 2 needs to perform vertical displacement, the motor one can be rotated to drive the transmission rod 17 to rotate, drive the sliding block 19 to rotate, drive the screw rod 14 to rotate through the cooperation of the sliding block 19 and the slot, at this time, the rotating screw rod 14 will drive the driving sleeve 13 to perform vertical displacement, drive the fishing block 2 to perform vertical displacement, and when the motor one fails after long time use, cannot drive the screw rod 14 to rotate, the poking block can be pulled to drive the poking block to perform vertical displacement, drive the sliding block 19 to perform vertical displacement, when the sliding block 19 is separated from the inside of the slot, the screw rod 14 can be manually poked to drive the screw rod 14 to rotate.

[0033] Embodiment 3

[0034] In combination with Figure 3The embodiment is further improved on the basis of embodiment 1, and the inner wall of the accommodation cavity 5 far away from the opening 6 is provided with a loading groove 9, the inner wall of the top of the loading groove 9 is fixedly provided with a motor two, the motor two is a forward and reverse stepping motor, the output end of the motor two is drivingly connected with a threaded rod 10, the other end of the threaded rod 10 is rotatably connected to the inner wall of the bottom end of the loading groove 9, the outer wall of the threaded rod 10 is threadedly connected with a plurality of threaded sleeves 11 corresponding to the opening 6, the outer wall of the threaded sleeve 11 is hingedly connected with a driving plate 8 hingedly connected to the inner side of the adjacent sealing block 7, the rotation of the motor two can drive the threaded rod 10 to rotate, the rotation of the threaded rod 10 will drive the threaded sleeve 11 to vertically displace, at this time the threaded sleeve 11 vertically displacing will drive the driving plate 8 to deflect, at this time the driving plate 8 deflecting will drive the sealing block 7 to deflect, at this time the sealing block 7 deflecting can open or close the opening 6.

[0035] The sliding block 19 is a rectangular block, the sliding groove 18 and the insertion groove are rectangular grooves, and the sliding block 19, the sliding groove 18 and the insertion groove are rectangular structures, so that the sliding block 19 can be driven to rotate when the transmission rod 17 rotates, and the screw rod 14 can be driven to rotate.

[0036] The outer side of the motor one is sleeved with a protection shell one fixed on the adjacent connecting block 15, and the outer side of the motor two is sleeved with a protection shell two fixed on the inner wall of the loading groove 9, the motor one can be protected by the protection shell one, the protection shell one is provided with an adapter hole one, and the transmission rod 17 rotates in the adapter hole one, the motor two can be protected by the protection shell two, the protection shell two is provided with an adapter hole two, and the threaded rod 10 rotates in the adapter hole two.

[0037] The outer wall of the screw rod 14 in the stabilizing block 16 is fixedly provided with a plurality of limiting blocks, and the inner wall of the stabilizing block 16 is provided with an annular groove, and the limiting blocks slide in the annular groove, so that the screw rod 14 can be limited by the cooperation of the limiting blocks and the annular groove, and the screw rod 14 can be prevented from deviating.

[0038] Embodiment 4

[0039] Combined Figure 5The embodiment is further improved on the basis of embodiment 1, wherein the connecting block 15 is provided with a through hole, a positioning block 20 is slidably arranged in the through hole, the outer side of the handle 1 is provided with a positioning groove 21 corresponding to the connecting block 15, one end of the positioning block 20 is slidably arranged in the adjacent positioning groove 21, the other end of the positioning block 20 is fixedly provided with a handle, a plurality of reset springs 12 are fixedly arranged on the connecting block 15 and close to the handle, and when the user needs to disassemble the sampling assembly from the device during use of the device, the positioning block 2 can be pulled to drive the positioning block 2 to displace, the connecting block 15 can be pulled to drive the connecting block 15, the trapping block 2 and the stabilizing block 16 and other assemblies to vertically displace when the positioning block 2 is separated from the inside of the positioning groove 21, and when the connecting block 15, the trapping block 2 and the stabilizing block 16 and other assemblies are separated from the handle 1, the sampling assembly is disassembled at this time, and the user can conveniently use the device.

[0040] Embodiment 5

[0041] Combined Figure 7 The embodiment is further improved on the basis of embodiment 1, wherein the outer side of the handle 1 is hingedly connected with a movable door 22 communicated with the receiving cavity 5, the outer side of the movable door 22 is provided with a sealing ring, the user can take out the sampling box in the receiving cavity 5 by opening the movable door 22, so as to facilitate the staff to monitor the growth status of the zebrafish larvae.

[0042] The outer side of the screw rod 14 is fixedly sleeved with a pushing block, the outer side of the pushing block is fixedly sleeved with a rubber sleeve, and the screw rod 14 can be driven to rotate by rotating the pushing block.

[0043] The width of the handle 1 is greater than the width of the guide block 4, the outer side of the top of the handle 1 is provided with anti-skid lines, and the outer side of the sampling box is attached with a label, so that the user can conveniently hold the handle 1.

[0044] Working principle: when the zebrafish larvae need to be sampled, the handle 1 can be held by hand, the handle 1 is placed into the breeding equipment, at this time the fishing mouth 3 on the fishing block 2 can be aimed at the zebrafish larvae that need to be fished, the zebrafish larvae are fished into the fishing mouth 3, when the zebrafish larvae pass through the fishing mouth 3 and enter the inside of the guide block 4, at this time the rotation of the motor one drives the driving link 17 to rotate, drives the sliding block 19 to rotate, through the cooperation of the sliding block 19 and the slot, the driving screw 14 is driven to rotate, at this time the rotating screw 14 will drive the driving sleeve 13 to vertically displace, drive the fishing block 2 to vertically displace, drive the guide block 4 to vertically displace upwards, drive the zebrafish larvae inside the guide block 4 to vertically displace upwards, when the guide block 4 corresponds to the opening 6, through the operation of the motor two, the threaded rod 10 can be driven to rotate, through the rotation of the threaded rod 10, the threaded sleeve 11 will be driven to vertically displace, at this time the vertically displaced threaded sleeve 11 will drive the driving plate 8 to deflect, at this time the deflected driving plate 8 will drive the closing block 7 to deflect, when the closing block 7 opens the opening 6, at this time the zebrafish larvae located in the inside of the guide block 4 will enter the inside of the storage cavity 5 through the opening 6, fall into the corresponding sampling box, at this time the closing block 7 deflects, when the closing block 7 closes the opening 6, at this time the above work is repeated until the zebrafish larvae sampling is completed, when the zebrafish larvae sampling is completed, by opening the movable door 22, the user can take out the sampling box inside the storage cavity 5, so that the growth of the zebrafish larvae is monitored, and when the motor one fails after a long time of use, the screw 14 cannot be driven to rotate, the toggle block can be pulled to drive the toggle block to vertically displace, drive the sliding block 19 to vertically displace, when the sliding block 19 is separated from the inside of the slot, the screw 14 can be manually rotated to drive the screw 14 to rotate, and when the user needs to disassemble the sampling assembly from the equipment during use, the positioning block 2 can be pulled to drive the positioning block 2 to displace, when the positioning block 2 is separated from the inside of the positioning slot 21, the connecting block 15 can be pulled to drive the connecting block 15, the fishing block 2 and the stabilizing block 16 and other assemblies to vertically displace, when the connecting block 15, the fishing block 2 and the stabilizing block 16 and other assemblies are separated from the handle 1, at this time the sampling assembly is disassembled, at this time the user can conveniently use the equipment.

[0045] The above embodiments are only preferred embodiments of the present application, and cannot be used to limit the scope of protection of the present application, any non-essential changes and replacements made by those skilled in the art on the basis of the present application shall fall within the scope of protection of the present application.

Claims

1. A sampling device for juvenile zebrafish, comprising a handle, characterized in that, A catching block is movably fitted onto the outer end of the handle. The catching block has a catching opening. Connecting blocks that are movably fitted onto the outside of the handle are provided on both sides of the catching block. A guide block is also fixed at the bottom of the catching block. The handle has a storage cavity. Multiple openings communicating with the storage cavity are provided on the side of the handle near the catching opening. A closing block for closing the opening is hinged to the inner wall of the top of the opening. A sample box that slides inside the storage cavity is provided below the opening. A support frame fixed to the inner wall of the storage cavity is provided at the bottom of the sample box. The end of the fishing block away from the fishing opening is fitted with a drive sleeve. A screw is threaded through the internal thread of the drive sleeve. One end of the screw is rotatably connected to a connecting block located at the tail end of the handle. The other end of the screw is movably fitted with a stabilizing block that slides outside the handle. A motor is fixed on the connecting block at the top of the handle. A transmission rod is driven to the output end of the motor. A groove is opened at the end of the transmission rod away from the motor. A slider is slidably connected inside the groove. A slot is opened at the end of the screw away from the connecting block. The end of the slider away from the transmission rod extends out of the groove and slides inside the slot. A toggle block is fixed to the slider located outside the groove and slot. The inner wall of the storage cavity away from the opening is provided with a loading groove. A second motor is fixed to the inner wall of the top of the loading groove. The output end of the second motor is connected to a threaded rod. The other end of the threaded rod is rotatably connected to the inner wall of the bottom of the loading groove. The outer wall of the threaded rod is threaded with multiple threaded sleeves corresponding to the opening. The outer wall of the threaded sleeve is hinged to a drive plate whose other end is hinged to the inner side of an adjacent closed block.

2. The zebrafish juvenile sampling device as described in claim 1, characterized in that, The slider is a rectangular block, and the groove and slot are both rectangular grooves.

3. The zebrafish juvenile sampling device as described in claim 1, characterized in that, The outer side of motor one is fitted with a protective shell one fixed to the adjacent connecting block, and the outer side of motor two is fitted with a protective shell two fixed to the inner wall of the loading groove.

4. The zebrafish juvenile sampling device as described in claim 1, characterized in that, Multiple limiting blocks are fixed to the outer wall of the screw located inside the stabilizing block. The inner wall of the stabilizing block has an annular groove, and the limiting blocks slide inside the annular groove.

5. The zebrafish juvenile sampling device as described in claim 1, characterized in that, The connecting block has a through hole, and a positioning block slides through the through hole. The outer side of the handle has a positioning groove corresponding to the connecting block. One end of the positioning block slides in the adjacent positioning groove, and the other end of the positioning block is fixed with a handle. The side of the handle near the connecting block has multiple return springs with their other ends fixed to the connecting block.

6. The zebrafish juvenile sampling device as described in claim 1, characterized in that, The handle is hinged to a movable door that communicates with the storage cavity, and a sealing ring is provided on the outside of the movable door.

7. The zebrafish juvenile sampling device as described in claim 1, characterized in that, An actuating block is fixedly sleeved on the outer side of the screw, and a rubber sleeve is fixedly sleeved on the outer side of the actuating block.

8. The zebrafish juvenile sampling device as described in claim 1, characterized in that, The width of the handle is greater than the width of the guide block, and the outer side of the top of the handle is provided with anti-slip texture. A label is affixed to the outer side of the lofting box.

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