Sample bottle and method of using the same

Abstract

The present application relates to the technical field of sample collection containers, and in particular to a sample bottle and its use method, comprising an outer bottle body, an inner bottle body, a primary filter, a top cover, a secondary filter, and a bottom cover. The inner cavity of the outer bottle body is divided by a preset layered plate to form a first space and a second space arranged in an upper and lower manner. The inner wall of the outer bottle body is located in the first space region and is provided with a falling channel that runs through the second space. The inner bottle body is embedded in the first space. The primary filter is sealed at the bottom of the inner bottle body and is used to filter cell clusters and impurities. The top cover is sealed at the top of the outer bottle body. The secondary filter is embedded in the second space and is used to filter interfering cells and waste liquid. The bottom cover is sealed at the bottom of the outer bottle body, and the bottom cover is provided with a negative pressure channel. The present application accelerates the filtration efficiency of the secondary filter through negative pressure suction, and ultimately can enrich more target cells in a short time, which is beneficial to the conduct of the experiment.

Description

Technical Field

[0001] The present application relates to the technical field of sample collection containers, and in particular to a sample bottle and a method for using the same. Background Art

[0002] Liquid-based cytology examinations involve four stages: sampling, slide preparation, staining, and observation and diagnosis. Each step requires corresponding in vitro diagnostic equipment, including sample transfer machines, centrifuges, liquid-based shakers, and slide preparation and staining machines. During pre-staining, centrifugation or sedimentation is required to enrich sample cells to meet staining requirements.

[0003] Chinese patent publication number CN113145191A discloses a sample bottle, comprising a bottle body having an upper opening, a filter screen disposed within the bottle body, the filter screen dividing the bottle body into a first space and a second space; a side of the upper opening opposite to the first space is a sample placement side, and a side of the upper opening opposite to the second space is a sample extraction side; the bottle body comprises an inner bottle and an outer bottle, the inner bottle being placed within the outer bottle and being located on one side of the outer bottle, forming a first space within the inner bottle and a second space between the inner and outer bottles, the filter screen being disposed on the inner bottle; the filter screen The inner bottle is provided with a bottom portion and is located opposite the second space. The bottom portion of the inner bottle is lower on the side closest to the second space and higher on the side away from the second space, forming an inclined second bottom. The outer bottle is provided with a first bottom portion, the first bottom portion and the second bottom portion being inclined in the same direction. The second bottom portion is provided with a lower opening, and a filter is provided at the lower opening. The inner wall of the outer bottle is provided with a first anti-reflection projection. The outer bottle also includes a bottle cap, which cooperates with the upper opening. The radially inner side of the upper surface of the bottle cap is partially concave to form an inner groove, and the inner groove is at least opposite to the second space of the bottle. The sample bottle combines traditional sample collection and enrichment into a single sample bottle, facilitating fully automated cell preparation and staining. During testing, a sample is placed from the sample placement side of the sample bottle, a pipette is inserted from the sample extraction side, and the reagent in the sample bottle is repeatedly aspirated through the pipette to separate target cells from the sample. The sample is then extracted with the pipette for enrichment and transfer. This structure facilitates sample transfer and enrichment, and is relatively simple in structure and more convenient to operate.

[0004] However, the sample bottle can only filter impurities / cell clusters through the filter, and cannot filter interfering cells (such as inflammatory cells) / waste liquid with smaller diameters. It also relies on manual pipette suction to achieve enrichment, which is cumbersome and inefficient, ultimately affecting the experimental results and even leading to experimental failure. Therefore, further improvements can be made. Summary of the Invention

[0005] In order to enrich more target cells in a short period of time, remove interfering cells with smaller diameters and waste liquid, and improve experimental efficiency and accuracy, the present application provides a sample bottle and a method for using the same.

[0006] In the first aspect, the present application provides a sample bottle, which is achieved by adopting the following technical solutions:

[0007] A sample bottle comprises an outer bottle body, an inner bottle body, a primary filter, a top cover, a secondary filter and a bottom cover. The inner cavity of the outer bottle body is divided into a first space and a second space arranged up and down by a preset layered plate. The inner wall of the outer bottle body is located in the first space area and is fixed with a falling channel that runs through the second space. The inner bottle body is embedded in the first space. The primary filter is sealed on the bottom of the inner bottle body and is used to filter cell clusters and impurities. The top cover is sealed on the top of the outer bottle body. The secondary filter is embedded in the second space and is used to filter interfering cells and waste liquid. The bottom cover is sealed on the bottom of the outer bottle body, and the bottom cover is provided with a negative pressure channel.

[0008] By adopting the above technical solution, impurities / cell clusters are first filtered and intercepted by the first-level filter, and then the interfering cells / waste liquid are filtered and eliminated by the second-level filter, leaving the target cells. The filtration efficiency of the second-level filter is accelerated by negative pressure suction, and ultimately more target cells can be enriched in a short time, which is conducive to the progress of the experiment.

[0009] Optionally, a liquid suction port is provided at a position on the top of the inner bottle body corresponding to the first space, and a liquid discharge port is provided at a position on the top of the inner bottle body corresponding to the falling channel.

[0010] By adopting the above technical solution, the preset pipette is inserted from the liquid suction port into the first space of the outer bottle body to extract the sample water mixed with the sample cells; the preset pipette is inserted from the liquid discharge port into the falling channel of the outer bottle body to transfer the sample water mixed with the sample cells to the second space of the outer bottle body.

[0011] Optionally, the secondary filter is slidably embedded in the second space, and the bottom cover is provided with a sealing assembly; the sealing assembly includes a sealing ring sleeved on the bottom cover, and the sealing ring abuts against the bottom of the outer bottle body.

[0012] By adopting the above technical solution, the sealing ring can improve the sealing performance of the connection between the bottom cover and the outer bottle body, and can better create a negative pressure environment when the external negative pressure equipment is started.

[0013] Optionally, the secondary filter is threadedly embedded in the second space, and the bottom cover is provided with a sealing assembly; an annular space distributed along the center of the bottom cover is provided inside the bottom cover, a first channel connected to the top of the annular space is provided at the top of the bottom cover, a second channel connected to the bottom of the annular space is provided at the bottom of the bottom cover, and a third channel connected to one side of the annular space is provided on one side of the bottom cover, and the third channel is arranged at an angle; the sealing assembly includes an outer annular sealing sheet and an inner annular sealing sheet, one end of the outer annular sealing sheet is slidably embedded in the third channel, and the other end extends obliquely toward the bottom of the bottom cover to the annular space, and one end of the inner annular sealing sheet is fixed to the inner wall of the annular space, and the other end extends obliquely toward the bottom of the bottom cover to abut against the outer annular sealing sheet.

[0014] By adopting the above technical solution, when an external negative pressure device is connected to the second channel to allow the air between the secondary filter and the bottom cover to be discharged from the first channel to the second channel, the flowing gas can squeeze the outer annular sealing sheet and the inner annular sealing sheet from the first channel to the second channel, thereby forming a negative pressure environment between the secondary filter and the bottom cover; when the external negative pressure device is removed, there is an air pressure difference between the first channel and the second channel, and the outer annular sealing sheet and the inner annular sealing sheet are pressed against each other under the action of the air pressure difference, thereby realizing the function of the sealing component to enable the air to be discharged only along the direction from the first channel to the second channel.

[0015] Optionally, the outer annular sealing sheet and the inner annular sealing sheet are both abutted at one end and have sealing teeth fixed on one side opposite to each other, and the two groups of sealing teeth are cross-engaged and matched with each other.

[0016] By adopting the above technical solution, the cross-engaging and matching sealing teeth can improve the sealing between the outer annular sealing sheet and the inner annular sealing sheet, so that when the external negative pressure equipment is removed, gas leakage between the outer annular sealing sheet and the inner annular sealing sheet is not likely to occur.

[0017] Optionally, a capacity expansion groove is provided on one side of the bottom cover corresponding to the position of the third channel, and a sealing strip adapted to the capacity expansion groove is fixedly provided on one end of the outer annular sealing sheet away from the inner annular sealing sheet.

[0018] By adopting the above technical solution, when the outer annular sealing sheet and the inner annular sealing sheet are pressed against each other under the action of the air pressure difference, both will produce a slight deformation, so that the outer annular sealing sheet has the force and tendency to move outward along the third channel, so that the sealing strip at one end of the outer annular sealing sheet is pressed against the inner wall of the second space, thereby improving the sealing between the outer bottle body and the bottom cover.

[0019] Optionally, the secondary filter includes a filter frame and a nuclear pore membrane sealed at the bottom of the filter frame, and the interior of the filter frame is divided into multiple areas by a preset partition plate.

[0020] By adopting the above technical solution, the partition plate divides the filter frame into multiple areas, which can prevent the sample water mixed with sample cells from accumulating easily and make it more dispersed, which is conducive to ensuring the filtration efficiency.

[0021] Optionally, a fool-proof notch is provided at the bottom of the outer bottle body.

[0022] By adopting the above technical solution and the design of the anti-mistake notch at the bottom of the outer bottle body, the error of installation direction when used on the equipment can be avoided as much as possible.

[0023] Optionally, a plurality of evenly distributed anti-slip ridges are provided on the periphery of the top cover.

[0024] By adopting the above technical solution, the anti-slip convex strip can increase hand friction, making it easier for the top cover to be opened by rotating the thread.

[0025] In a second aspect, the present application provides a method for using a sample bottle, which is applied to the above-mentioned sample bottle and includes the following steps:

[0026] S1. Open the top cover and dip the sampling brush into the sample water reserved in the inner bottle body to mix the sample cells with the sample water. At this time, part of the sample water mixed with the sample cells passes through the primary filter, and the primary filter filters and intercepts impurities / cell clusters in this process; S2. Insert the preset pipette from the liquid suction port into the first space of the outer bottle body to extract the sample water mixed with the sample cells; S3. Insert the pipette from the liquid discharge port into the falling channel of the outer bottle body to transfer the sample water mixed with the sample cells to the second space of the outer bottle body; S4. Start the external negative pressure device and connect the negative pressure device to the negative pressure channel. At this time, the sample water mixed with the sample cells passes through the secondary filter, and the secondary filter filters and removes interfering cells / waste liquid in this process, leaving the target cells; S5. Open the bottom cover and remove the secondary filter.

[0027] By adopting the above technical solution, impurities / cell clusters are first filtered and intercepted by the first-level filter, and then the interfering cells / waste liquid are filtered and eliminated by the second-level filter, leaving the target cells. The filtration efficiency of the second-level filter is accelerated by negative pressure suction, and ultimately more target cells can be enriched in a short time, which is conducive to the progress of the experiment.

[0028] In summary, this application has at least the following beneficial technical effects:

[0029] 1. First, the impurities / cell clusters are filtered and intercepted through the primary filter, and then the interfering cells / waste liquid are filtered and removed through the secondary filter, leaving the target cells. The filtration efficiency of the secondary filter is accelerated by negative pressure suction, and ultimately more target cells can be enriched in a short time, which is beneficial to the experiment.

[0030] 2. The partition plate divides the filter frame into multiple areas, which can prevent the sample water mixed with sample cells from accumulating easily and make it more dispersed, which is conducive to ensuring the filtration efficiency;

[0031] 3. The sealing ring can improve the sealing performance of the connection between the bottom cover and the outer bottle body, and can better create a negative pressure environment when the external negative pressure equipment is started. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the overall structure of Example 1 of the present application.

[0033] Figure 2 This is a cross-sectional view of Example 1 of the present application.

[0034] Figure 3 It is a schematic diagram of the explosion structure of Example 1 of the present application.

[0035] Figure 4 This is a schematic diagram of the exploded structure of Example 1 of the present application to illustrate the position of the layered plates.

[0036] Figure 5 It is a cross-sectional view of the bottom cover and the sealing assembly in Example 2 of the present application.

[0037] Explanation of the accompanying drawings: 1. Outer bottle body; 10. Layered plate; 11. First space; 12. Second space; 13. Falling channel; 14. Anti-fool notch; 2. Inner bottle body; 3. Primary filter; 4. Top cover; 41. Anti-slip ridge; 5. Secondary filter; 51. Filter frame; 52. Nuclear pore membrane; 53. Partition plate; 6. Bottom cover; 60. Annular space; 61. First channel; 62. Second channel; 63. Third channel; 64. Expansion groove; 7. Negative pressure channel; 81. Liquid suction port; 82. Liquid discharge port; 9. Sealing assembly; 91. Sealing ring; 92. Outer annular sealing plate; 93. Inner annular sealing plate; 94. Sealing teeth; 95. Sealing strip. DETAILED DESCRIPTION

[0038] The following is combined with Figure 1-5 This application is described in further detail.

[0039] The embodiment of the present application discloses a sample bottle.

[0040] Example 1:

[0041] Reference Figure 1-2The sample bottle includes an outer bottle body 1, an inner bottle body 2, a primary filter 3, a top cover 4, a secondary filter 5 and a bottom cover 6; wherein, a layer plate 10 is fixedly provided in the inner cavity of the outer bottle body 1, and the layer plate 10 divides the inner cavity of the outer bottle body 1 into a first space 11 and a second space 12 arranged up and down, and a falling channel 13 is fixedly provided on the inner wall of the outer bottle body 1 in the area of ​​the first space 11, and the falling channel 13 passes through the layer plate 10 and is connected to the second space 12.

[0042] Reference Figure 2-4 The inner bottle body 2 is slidably embedded in the first space 11 from top to bottom. The bottom of the inner bottle body 2 is an inclined opening. The first-level filter 3 adopts a nylon membrane that can filter cell clusters and impurities. The first-level filter 3 is fixedly sealed to the opening at the bottom of the inner bottle body 2; the top cover 4 is fixedly sealed to the top of the outer bottle body 1 by a threaded connection. The secondary filter 5 adopts a nuclear pore membrane 52 that can filter interfering cells and waste liquid. The secondary filter 5 is slidably embedded in the second space 12; the bottom cover 6 is sealed to the bottom of the outer bottle body 1 by a plug-in connection, and the bottom cover 6 is provided with a negative pressure channel 7.

[0043] A liquid suction port 81 is provided at a position on the top of the inner bottle body 2 corresponding to the first space 11 , and a liquid discharge port 82 is provided at a position on the top of the inner bottle body 2 corresponding to the falling channel 13 .

[0044] During use: S1. Open the top cover 4 and dip the sampling brush into the sample water reserved in the inner bottle body 2 to mix the sample cells with the sample water. At this time, part of the sample water mixed with the sample cells passes through the primary filter 3, and the primary filter 3 filters and intercepts impurities / cell clusters in this process; S2. Insert the pipette from the liquid suction port 81 into the first space 11 of the outer bottle body 1 to extract the sample water mixed with the sample cells; S3. Insert the preset pipette from the liquid discharge port 82 into the falling channel 13 of the outer bottle body 1 to transfer the sample water mixed with the sample cells to the second space 12 of the outer bottle body 1; S4. Start the external negative pressure device and connect the negative pressure device to the negative pressure channel 7. At this time, the sample water mixed with the sample cells passes through the secondary filter 5, and the secondary filter 5 filters and removes interfering cells / waste liquid in this process, leaving the target cells; S5. Open the bottom cover 6 and remove the secondary filter 5.

[0045] First, the impurities / cell clusters are filtered and intercepted by the primary filter 3, and then the interfering cells / waste liquid are filtered and eliminated by the secondary filter 5, leaving the target cells. The filtration efficiency of the secondary filter 5 is accelerated by negative pressure suction, and finally more target cells can be enriched in a short time, which is beneficial to the experiment.

[0046] In this embodiment, the secondary filter 5 includes a filter frame 51 and a nucleopore membrane 52 fixedly sealed to the bottom of the filter frame 51. The interior of the filter frame 51 is divided into multiple areas by a pre-set partition plate 53. The partition plates 53 divide the filter frame 51 into multiple areas, which can prevent the sample water mixed with the sample cells from accumulating and make it more dispersed, thereby ensuring filtration efficiency.

[0047] In this embodiment, the outer contour of the filter frame 51 of the secondary filter 5 matches the inner contour of the second space 12. The secondary filter 5 is slidably embedded in the second space 12. The bottom cover 6 is provided with a sealing assembly 9, which includes a sealing ring 91 that is sleeved on the bottom cover 6. The bottom cover 6 has a stepped shape, so that when the bottom cover 6 is plugged into the bottom of the outer bottle body 1, the sealing ring 91 abuts against the bottom of the outer bottle body 1. The sealing ring 91 improves the sealing between the bottom cover 6 and the outer bottle body 1, and can better create a negative pressure environment when the external negative pressure device is activated.

[0048] In this embodiment, the bottom of the outer bottle body 1 is provided with an anti-mistake notch 14. The design of the anti-mistake notch 14 is to avoid the wrong installation direction when using it on the device.

[0049] In this embodiment, a plurality of evenly distributed anti-skid ridges 41 are provided on the outer periphery of the top cover 4. The anti-skid ridges 41 can increase hand friction and facilitate the threaded rotation of the top cover 4 to open it.

[0050] Example 2:

[0051] The difference between this embodiment and embodiment 1 lies in the structure of the sealing assembly 9 .

[0052] Reference Figure 2 、 3 5. In this embodiment, the outer contour of the filter frame 51 of the secondary filter 5 is consistent with the inner contour of the second space 12, and the secondary filter 5 is threadedly embedded in the second space 12. It is worth mentioning that the secondary filter 5 in Example 1 adopts a sliding embedding method, and its shape may be non-circular, that is, the bottom of the outer bottle body 1 in Example 1 may be provided with an anti-fool notch 14, and the secondary filter 5 in Example 2 adopts a threaded embedding method, and its shape needs to be circular; an annular space 60 distributed along the center is provided inside the bottom cover 6, and a first channel 61 connected to the top of the annular space 60 is provided at the top of the bottom cover 6, and the first channel 61 is a circular hole. A second channel 62 connected to the bottom of the annular space 60 is provided at the bottom of the bottom cover 6, and the second channel 62 is a circular hole. A third channel 63 connected to one side of the annular space 60 is provided on one side of the bottom cover 6, and the third channel 63 is an annular hole, and the third channel 63 is arranged at an angle.

[0053] Similarly, the bottom cover 6 is provided with a sealing assembly 9, which includes an outer annular sealing sheet 92 and an inner annular sealing sheet 93; wherein, the outer annular sealing sheet 92 and the inner annular sealing sheet 93 are both annular structures and have a sheet cross-section, one end of the outer annular sealing sheet 92 is slidably embedded in the third channel 63, and the other end is inclined toward the bottom of the bottom cover 6 and extends to the center of the annular space 60; one end of the inner annular sealing sheet 93 is fixed to the inner wall of the annular space 60, and the other end is inclined toward the bottom of the bottom cover 6 and extends to the center of the annular space 60 and abuts against the outer annular sealing sheet 92, that is, the outer annular sealing sheet 92 and the inner annular sealing sheet 93 together form a one-way exhaust structure similar to an inverted "human" structure. When an external negative pressure device is connected to the second channel 62 to allow the air between the secondary filter 5 and the bottom cover 6 to be discharged from the first channel 61 to the second channel 62, the flowing gas can squeeze the outer annular sealing sheet 92 and the inner annular sealing sheet 93 from the first channel 61 to the second channel 62, thereby forming a negative pressure environment between the secondary filter 5 and the bottom cover 6; when the external negative pressure device is removed, there is an air pressure difference between the first channel 61 and the second channel 62, and the outer annular sealing sheet 92 and the inner annular sealing sheet 93 are pressed against each other under the action of the air pressure difference, thereby realizing the function of the sealing component 9 to allow the air to be discharged only along the direction from the first channel 61 to the second channel 62.

[0054] In this embodiment, sealing teeth 94 are fixed to the opposing ends of the outer annular sealing sheet 92 and the inner annular sealing sheet 93, and the two sets of sealing teeth 94 interlock and fit together. The interlocking and fitting sealing teeth 94 improve the sealing between the outer annular sealing sheet 92 and the inner annular sealing sheet 93, thereby preventing gas leakage between the outer annular sealing sheet 92 and the inner annular sealing sheet 93 when the external negative pressure device is removed.

[0055] In this embodiment, a capacity expansion groove 64 is formed on one side of the bottom cover 6 at a position corresponding to the third channel 63. A sealing strip 95 is integrally formed and fixed to the end of the outer annular sealing sheet 92 away from the inner annular sealing sheet 93, and the sealing strip 95 is adapted to the capacity expansion groove 64. When the outer annular sealing sheet 92 and the inner annular sealing sheet 93 press against each other under the action of the air pressure difference, both will slightly deform, giving the outer annular sealing sheet 92 a force and tendency to move outward along the third channel 63. As a result, the sealing strip 95 at one end of the outer annular sealing sheet 92 presses against the inner wall of the second space 12, thereby improving the sealing between the outer bottle body 1 and the bottom cover 6.

[0056] The present application also discloses a method for using a sample bottle, comprising the following steps:

[0057] S1. Open the top cover 4 and dip the sampling brush into the sample water reserved in the inner bottle 2 to mix the sample cells with the sample water. At this time, part of the sample water mixed with the sample cells passes through the primary filter 3, and the primary filter 3 filters and intercepts impurities / cell clusters in this process;

[0058] S2. Inserting a preset pipette into the first space 11 of the outer bottle body 1 through the liquid suction port 81 to extract the sample water mixed with the sample cells;

[0059] S3, inserting the pipette into the falling channel 13 of the outer bottle body 1 through the liquid discharge port 82 to transfer the sample water mixed with the sample cells to the second space 12 of the outer bottle body 1;

[0060] S4. Start the external negative pressure device and connect it to the negative pressure channel 7. At this time, the sample water mixed with the sample cells passes through the secondary filter 5. The secondary filter 5 filters out the interfering cells and waste liquid in this process, leaving the target cells.

[0061] S5. Open the bottom cover 6 and remove the secondary filter 5.

[0062] The embodiments of this specific implementation method are all preferred embodiments of the present application and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be covered within the scope of protection of the present application.

Claims

1. A sample bottle, characterized in that: The invention comprises an outer bottle body (1), an inner bottle body (2), a primary filter (3), a top cover (4), a secondary filter (5) and a bottom cover (6); the inner cavity of the outer bottle body (1) is divided by a preset layered plate (10) to form a first space (11) and a second space (12) arranged in an upper and lower manner; the inner wall of the outer bottle body (1) is provided with a falling channel (13) extending through the second space (12) in the region of the first space (11); the inner bottle body (2) is embedded in the first space (11); the primary filter (3) is sealed on the inner bottle body (6); The bottom of the inner bottle body (2) is provided with a liquid suction port (81) at a position corresponding to the first space (11), and the top of the inner bottle body (2) is provided with a liquid discharge port (82) at a position corresponding to the falling channel (13).

2. A sample bottle according to claim 1, characterized in that: The secondary filter (5) is slidably embedded in the second space (12), and the bottom cover (6) is provided with a sealing assembly (9); the sealing assembly (9) includes a sealing ring (91) sleeved on the bottom cover (6), and the sealing ring (91) abuts against the bottom of the outer bottle body (1).

3. The sample bottle according to claim 1, characterized in that: The secondary filter (5) is threadedly embedded in the second space (12), and the bottom cover (6) is provided with a sealing assembly (9); an annular space (60) distributed along the center of the bottom cover (6) is provided inside the bottom cover (6), a first channel (61) connected to the top of the annular space (60) is provided on the top of the bottom cover (6), a second channel (62) connected to the bottom of the annular space (60) is provided on the bottom of the bottom cover (6), and a third channel (63) connected to one side of the annular space (60) is provided on one side of the bottom cover (6). The third channel (63) is arranged obliquely; the sealing assembly (9) comprises an outer annular sealing sheet (92) and an inner annular sealing sheet (93); one end of the outer annular sealing sheet (92) is slidably embedded in the third channel (63), and the other end is inclined and extended toward the bottom of the bottom cover (6) to the annular space (60); one end of the inner annular sealing sheet (93) is fixed to the inner wall of the annular space (60), and the other end is inclined and extended toward the bottom of the bottom cover (6) to abut against the outer annular sealing sheet (92).

4. The sample bottle according to claim 3, characterized in that: The outer annular sealing sheet (92) and the inner annular sealing sheet (93) are in contact at one end and are both provided with sealing teeth (94) on one side opposite to each other, and the two groups of sealing teeth (94) are cross-engaged and matched with each other.

5. The sample bottle according to claim 3, characterized in that: An expansion groove (64) is provided on one side of the bottom cover (6) at a position corresponding to the third channel (63), and a sealing strip (95) adapted to the expansion groove (64) is fixedly provided on one end of the outer annular sealing sheet (92) away from the inner annular sealing sheet (93).

6. A sample bottle according to any one of claims 2 to 5, characterized in that: The secondary filter (5) comprises a filter frame (51) and a nucleus pore membrane (52) sealed at the bottom of the filter frame (51); the interior of the filter frame (51) is divided into a plurality of areas by a preset partition plate (53).

7. The sample bottle according to claim 2, characterized in that: The bottom of the outer bottle body (1) is provided with an anti-mistake notch (14).

8. The sample bottle according to claim 2, characterized in that: The outer periphery of the top cover (4) is provided with a plurality of evenly distributed anti-slip convex strips (41).

9. A method for using a sample bottle, comprising the sample bottle according to any one of claims 2 to 5, characterized in that: The following steps are also included: S1. Open the top cover (4), dip the sampling brush into the sample water reserved in the inner bottle (2), and mix the sample cells with the sample water. At this time, part of the sample water mixed with the sample cells passes through the primary filter (3), and the primary filter (3) filters and intercepts impurities / cell clusters in this process; S2, inserting a preset pipette into the first space (11) of the outer bottle body (1) through the liquid suction port (81) to extract the sample water mixed with the sample cells; S3, inserting the pipette into the falling channel (13) of the outer bottle body (1) through the liquid discharge port (82) to transfer the sample water mixed with the sample cells to the second space (12) of the outer bottle body (1); S4. Start the external negative pressure device and connect the negative pressure device to the negative pressure channel (7). At this time, the sample water mixed with the sample cells passes through the secondary filter (5). The secondary filter (5) filters out the interfering cells / waste liquid in this process, leaving the target cells. S5. Open the bottom cover (6) and remove the secondary filter (5).

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