Mitochondrial nutrient composition for improving glucose metabolism, preparation method and application thereof

Abstract

The present invention discloses a mitochondrial nutrient composition for improving glucose metabolism, a preparation method, and an application thereof. The nutrient composition comprises honokiol, pyrroloquinoline quinone, and sepiolite. The preparation method of the nutrient composition comprises the following steps: (1) preparing pyrroloquinoline quinone; (2) preparing honokiol; (3) sieving the sepiolite; and (4) uniformly mixing the pyrroloquinoline quinone, honokiol, and the sieved sepiolite. The prepared nutrient composition is used to prepare a fish feed additive. Compared with the prior art, the nutrient composition of the present invention uses pyrroloquinoline quinone and honokiol as main raw materials, and is scientifically formulated according to their different biological functions. Through the selected combination, it can target and regulate mitochondria, significantly reduce mitochondrial oxidative stress, stimulate mitochondrial metabolic activity, and enhance mitochondrial biosynthesis, ultimately improving mitochondrial function and providing abundant power for promoting glucose metabolism in fish.

Description

Technical Field

[0001] The present invention relates to nutrients, preparation methods and applications thereof, and in particular to a mitochondrial nutrient composition for improving sugar metabolism, and a preparation method and application thereof. Background Art

[0002] Compared to proteins and lipids, carbohydrates are the cheapest energy source. They also have a protein-sparing effect and can achieve certain environmental benefits by reducing ammonia nitrogen emissions in animals. Compared to mammals, fish have a relatively low utilization rate of carbohydrates due to their physiology. Studies have shown that glucose loading or excessively high dietary sugar levels can lead to persistent hyperglycemia in fish.

[0003] Mitochondria are basic organelles present in all types of cells, except for mammalian red blood cells. As the "generator" of the cell, mitochondria play a vital role in the cell's energy metabolism. At the same time, mitochondria are the main site of intracellular reactive oxygen species (ROS) production. The massive production of reactive oxygen species and the reduction of antioxidant defense capacity will change the cell's metabolism, ATP production, and the synthesis of nuclear and mitochondrial DNA encoding genes, ultimately leading to the occurrence of mitochondrial oxidative stress and damage to mitochondrial components. Usually, excessive glucose will cause the electron transport chain to excessively generate ROS through the hexosamine biosynthesis pathway, causing mitochondria to suffer oxidative stress, which in turn affects a series of physiological processes and is highly correlated with mitochondrial function damage. In addition, in mammals, a high-sugar diet leads to abnormal energy metabolism in the body and also causes mitochondrial dysfunction, ultimately inhibiting the synthesis and secretion of insulin by pancreatic β cells and promoting the onset of type 2 diabetes.

[0004] Studies have found that long-term feeding of a high-sugar diet significantly inhibits mitochondrial biogenesis and respiratory chain complex activity in the liver of the amblycephala bream, leading to mitochondrial dysfunction. Studies on the mechanisms of fish sugar metabolism based on transcriptomics and metabolomics also show that long-term feeding of a high-sugar diet can damage mitochondrial function in amblycephala bream, leading to metabolic disorders. These results suggest that mitochondrial dysfunction caused by a high-sugar diet may be an important cause of sugar metabolism disorders in fish.

[0005] At present, research on sugar metabolism in fish is still relatively superficial. Although mitochondrial dysfunction caused by a high-sugar diet has been confirmed in fish, there have been no reports of regulatory agents that target and enhance mitochondrial function in fish and thus improve sugar metabolism in fish. Summary of the Invention

[0006] Purpose of the invention: In order to solve the technical problems existing in the prior art, the present invention aims to provide a mitochondrial nutrient composition for improving glucose metabolism, as well as a preparation method and application.

[0007] Technical solution: The mitochondrial nutrient composition for improving glucose metabolism of the present invention comprises honokiol, pyrroloquinoline quinone and sepiolite.

[0008] Furthermore, the nutrient composition comprises, by mass, 4-25 parts of honokiol, 2-20 parts of pyrroloquinoline quinone and 455-499 parts of sepiolite, preferably, 13-22 parts of honokiol, 4-20 parts of pyrroloquinoline quinone and 455-483 parts of sepiolite, and more preferably, 15-20 parts of honokiol, 10-20 parts of pyrroloquinoline quinone and 460-470 parts of sepiolite.

[0009] Furthermore, the mass ratio of honokiol to pyrroloquinoline quinone is 1.5-2.25:1.

[0010] The method for preparing the mitochondrial nutrient composition for improving glucose metabolism of the present invention comprises the following steps:

[0011] (1) preparing pyrroloquinoline quinone;

[0012] (2) preparing honokiol;

[0013] (3) Screening of sepiolite;

[0014] (4) Evenly mix pyrroloquinoline quinone, magnolol and sieved sepiolite to obtain a nutrient composition.

[0015] Furthermore, the pyrroloquinoline quinone is prepared by a microbial fermentation method, and the original bacteria are methylotrophic bacteria, so that the pyrroloquinoline quinone is prepared in a clean, low-carbon, mild and controllable manner and the production cost is reduced.

[0016] Furthermore, the honokiol is prepared by ethanol extraction, and the extraction raw material is the traditional Chinese medicine Magnolia bark, so as to improve the extraction accuracy.

[0017] Furthermore, the sepiolite is sieved through a 60-mesh sieve to ensure the uniformity of the components.

[0018] The invention relates to an application of the mitochondrial nutrient composition for improving sugar metabolism in the preparation of a fish feed additive.

[0019] Principle of the invention: Honokiol is a small molecule polyphenol isolated from Magnolia officinalis. Studies have shown that it has activity as a free radical and lipid peroxidation inhibitor, thereby reducing oxidative stress and improving mitochondrial function by inhibiting mitochondrial protein acetylation. Pyrroloquinoline quinone is a coenzyme of oxidoreductase that can prevent cell damage caused by intracellular oxidative reactions and the production of reactive oxygen species by bioactive substances in vitro. Its unique feature is that it can increase the number and quality of healthy mitochondria, thereby increasing antioxidant activity and anti-apoptosis capacity by enhancing mitochondrial biogenesis. Both can provide sufficient nutrition and energy to mitochondria through nutritional means, effectively reducing free radical damage to mitochondria, activating mitochondrial biogenesis, increasing the number of mitochondria, restoring mitochondrial structure and quality, and enhancing mitochondrial function. They have great potential in regulating the body's glucose metabolism function. However, to date, there has been no report on the combined application of honokiol and pyrroloquinoline quinone in aquatic products, and pyrroloquinoline quinone has only been reported in mice with diabetic nephropathy and cardiovascular disease models.

[0020] By combining honokiol and pyrroloquinoline quinone in a scientific ratio and using sepiolite as a carrier, this invention has developed a nutrient composition that enhances mitochondrial function and thus improves glucose metabolism in aquatic animals. Application of this nutrient composition in aquaculture can specifically regulate mitochondria, more directly and effectively improving low carbohydrate utilization in fish, reducing the use of protein and lipids in aquatic feeds, thereby maximizing economic and environmental benefits.

[0021] Beneficial effects: Compared with the existing technology, the present invention has the following significant advantages: the mitochondrial nutrient composition for improving sugar metabolism of the present invention uses magnolia bark phenol and pyrroloquinoline quinone as the main raw materials, and makes full use of the different characteristics of their biological functions to carry out scientific compatibility to exert their synergistic effects. Through the selected combination, it can target and regulate mitochondria, significantly reduce mitochondrial oxidative stress, stimulate mitochondrial metabolic activity, and enhance mitochondrial biosynthesis, ultimately improving mitochondrial function and providing sufficient power to promote fish sugar metabolism, ultimately improving its sugar metabolic function and greatly improving the fish's utilization rate of sugars in feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The effects of different feeds on the weight gain rate of young bream are shown in the following figure:

[0023] Figure 2 The effects of different feeds on the feed conversion ratio of young Megalobrama amblycephala are shown in the figure.

[0024] Figure 3 The present invention is about the effects of different feeds on the catalase activity of liver mitochondria of juvenile amblycephala;

[0025] Figure 4The present invention is to show the effects of different feeds on the MDA content of liver mitochondria of juvenile amblycephala;

[0026] Figure 5 The present invention shows the effects of different feeds on the expression level of TFAM gene in the liver of Megalobrama amblycephala;

[0027] Figure 6 This is the effect of different feeds on the mtDNA expression level in the liver of Megalobrama amblycephala;

[0028] Figure 7 The figures show the effects of different feeds on glucose tolerance of Megalobrama amblycephala of the present invention. Lowercase letters indicate statistical differences between the same diet group at different sampling time points; * indicates statistical differences between different diet groups at the same sampling time point; ns P>0.05, *P<0.05, **P<0.01, ***P<0.001. DETAILED DESCRIPTION

[0029] The technical solution of the present invention will be further described below in conjunction with the embodiments and drawings.

[0030] Example 1: The sepiolite is crushed and passed through a 60-mesh sieve for use; then, 4 g of honokiol, 2 g of pyrroloquinoline quinone and 494 g of the sieved sepiolite are thoroughly mixed according to a step-by-step mixing method. This process requires that the mixture be uniform and stable, and then 500 g of a mitochondrial nutrient regulating composition for improving the sugar utilization efficiency of aquatic animals is obtained, which is used as an efficient and environmentally friendly feed additive.

[0031] Example 2: The sepiolite was crushed and passed through a 60-mesh sieve for use; then, 3 g of honokiol, 2 g of pyrroloquinoline quinone, and 495 g of the sieved sepiolite were thoroughly mixed according to a step-by-step mixing method. This process required that the mixture be uniform and stable. Subsequently, 500 g of a mitochondrial nutrient regulating composition for improving sugar utilization in aquatic animals was obtained, which was used as an efficient and environmentally friendly feed additive.

[0032] Example 3: The sepiolite was crushed and passed through a 60-mesh sieve for use; then, 4 g of honokiol, 3 g of pyrroloquinoline quinone and 493 g of the sieved sepiolite were thoroughly mixed according to a step-by-step mixing method. This process required that the mixture be uniform and stable. Subsequently, 500 g of a mitochondrial nutrient regulating composition for improving sugar utilization in aquatic animals was obtained, which was used as an efficient and environmentally friendly feed additive.

[0033] Example 4: The sepiolite was crushed and passed through a 60-mesh sieve for use; then, 18 g of honokiol, 8 g of pyrroloquinoline quinone and 474 g of the sieved sepiolite were thoroughly mixed according to a step-by-step mixing method. This process required that the mixture be uniform and stable, and then 500 g of a mitochondrial nutrient regulating composition for improving the sugar utilization efficiency of aquatic animals was obtained, which was used as an efficient and environmentally friendly feed additive.

[0034] Example 5: The sepiolite is crushed and passed through a 60-mesh sieve for use; then, 24 g of honokiol, 16 g of pyrroloquinoline quinone and 460 g of the sieved sepiolite are thoroughly mixed according to a step-by-step mixing method. This process requires that the mixture be uniform and stable. Subsequently, 500 g of a mitochondrial nutrient regulating composition for improving the sugar utilization efficiency of aquatic animals is obtained, which is used as an efficient and environmentally friendly feed additive.

[0035] Comparative Example 1: The sepiolite was crushed and passed through a 60-mesh sieve for use; then, 4 g of honokiol and 494 g of the sieved sepiolite were thoroughly mixed according to a step-by-step mixing method. This process required that the mixture be uniform and stable, and then 498 g of mitochondrial nutrient regulator I for improving the sugar utilization efficiency of aquatic animals was obtained.

[0036] Comparative Example 2: The sepiolite was crushed and passed through a 60-mesh sieve for use; then, 2 g of pyrroloquinoline quinone and 494 g of the sieved sepiolite were thoroughly mixed according to a step-by-step mixing method. This process required that the mixture be uniform and stable, and then 496 g of mitochondrial nutrient regulator II for improving sugar utilization in aquatic animals was obtained.

[0037] Experimental Example 1 Breeding Test and Analysis of Breeding Results

[0038] The breeding experiment was carried out in a recirculating aquaculture system at the Aquatic Teaching and Research Base of Nanjing Agricultural University (Xingdian Town, Pukou District, Nanjing). The fish used in the experiment were Megalobrama amblycephala, which were taken from the National Aquatic Original Farm in Ezhou City, Wuhan. After 2 weeks of temporary rearing, 320 strong and neatly sized juveniles (initial weight: 12.4±0.37g) were randomly placed into 20 breeding tanks (specifications: 300L), with 16 fish in each cage. The breeding experiment included five groups: control group, high sugar group, high sugar + regulator group I, high sugar + regulator group II and high sugar + regulatory combination group, with 4 replicates in each group. The sugar level in the control group was 30%, and the sugar level in the high sugar group was 43%, produced by Nanjing Hepalink Feed Co., Ltd. The high sugar + modulator I and high sugar + modulator II groups were fed a diet supplemented with 1.0% of the mitochondrial nutrient modulator I prepared in Comparative Example 1 and 1.0% of the mitochondrial nutrient modulator II prepared in Comparative Example 2, respectively. The high sugar + modulator combination group was fed a diet supplemented with 1.0% of the nutrient modulator combination prepared in Example 1. The feed formulas are shown in Table 1. The fish were reared for 12 weeks, during which time their feeding and mortality were observed and recorded.

[0039] After the breeding experiment, blood and liver samples were collected for index determination. The results are shown in Tables 2-3 and Figures 1-6 . Collect samples for analysis and determination.

[0040] Table 1 Test feed formula

[0041]

[0042] Table 2 Growth performance and blood biochemical indicators of Megalobrama amblycephala

[0043]

[0044]

[0045] Note: Data in the same industry with the same superscript letters have no significant difference. Otherwise, there is a significant difference.

[0046] Table 3 Mitochondrial complex activity of Megalobrama amblycephala

[0047]

[0048] Note: Data in the same industry with the same superscript letters have no significant difference. Otherwise, there is a significant difference.

[0049] Depend on Figure 1 、 Figure 2 As shown in Table 2, there was no significant difference in weight gain between the high sugar group and the control group (P>0.05), but its liver-to-body ratio and serum glucose, glycated serum protein, advanced glycation end products, insulin content, triglyceride and cholesterol content were also significantly higher than those of the control group (P<0.05). In addition, the high sugar group, high sugar + comparison example I group, high sugar + comparison example II group and high sugar + regulation composition significantly increased the weight gain rate (P<0.05). At the same time, compared with the high sugar group, the high sugar + regulation composition group significantly increased insulin levels (P<0.05), but the feed conversion ratio, serum glucose, glycated serum protein, advanced glycation end products, triglyceride and cholesterol content were significantly reduced (P<0.05). This shows that the use of feed supplemented with the sugar metabolism regulation composition prepared in Example 1 can significantly improve the growth performance of fish. In addition, the results of plasma biochemical related parameters show that after breeding with feed supplemented with the sugar metabolism regulation composition prepared in Example 1, the function of pancreatic β cells in fish can be significantly improved, and the secretion and release of insulin can be promoted. At the same time, feeding the fish with the high-sugar feed supplemented with the glucose metabolism regulating composition prepared in Example 1 also significantly reduced triglyceride and cholesterol levels. This is likely due to the conversion of excess glucose in the blood into lipids, which, by enhancing lipid synthesis, lowers plasma glucose levels, ultimately lowering blood glucose levels and thus maintaining glucose homeostasis in the fish. Furthermore, the peak values of the aforementioned indicators all occurred in the high-sugar + regulating composition group, indicating a synergistic effect between honokiol and pyrroloquinoline quinone.

[0050] Depend on Figure 3-Figure 6As shown in Table 3, compared with the control group, the high sugar group significantly increased the level of liver mitochondrial MDA and reduced the activity of mitochondrial CAT (P<0.05), and the addition of the sugar metabolism regulating composition prepared in Example 1 to the high sugar group significantly improved this situation. In addition, compared with the control group, the high sugar group significantly reduced the expression levels of liver TFAM and mtDNA genes and the activity of mitochondrial complexes I and V. Compared with the high sugar group, the high sugar group improved the above indicators after adding the mitochondrial nutrient regulators prepared by Comparative Examples 1 and 2, but the differences were not significant (P>0.05). However, after adding the sugar metabolism regulating composition prepared in Example 1 to the high sugar group, the expression levels of the above indicators were significantly increased (P<0.05). The above results show that high sugar causes oxidative stress in the liver mitochondria of fish, reduces the level of mitochondrial biosynthesis, and causes impaired mitochondrial function. After adding the sugar metabolism regulating composition prepared in Example 1, the antioxidant capacity of liver mitochondria is improved, the level of mitochondrial biosynthesis is increased, and ultimately the mitochondrial function is enhanced.

[0051] Test Example 2 Carp breeding test and result analysis

[0052] The breeding experiment was carried out at the Aquatic Teaching and Research Base of Nanjing Agricultural University (Xingdian Town, Pukou District, Nanjing). After temporary breeding, 192 carps with strong physique and neat specifications (initial weight: 15.7±0.39g) were randomly divided into three experimental groups, namely the control group, the high sugar group and the high sugar + regulating composition group (the same as Example 2). Each group had 4 circulating breeding tanks (specifications: 300L) and 16 fish in each cage. The feed formula is as shown in Table 4. After 3 months of cage breeding, blood and liver samples were collected for index determination. The results are shown in Tables 5-7 and Figure 7 . Then, glucose tolerance test was conducted using 6 circulating culture tanks. The results are shown in Figure 7 The relevant process is as follows: 4 fish in each tank were weighed and injected with 1.67 g / kg body weight of glucose solution, and blood samples were collected at 0, 1, 2, 4, 8 and 12 hours.

[0053] Table 4 Test feed formula

[0054]

[0055]

[0056] Table 5 Effects of different feeds on growth performance and blood biochemical indicators of carp

[0057]

[0058] Note: Data in the same industry with the same superscript letters have no significant difference. Otherwise, there is a significant difference.

[0059] Table 6 Mitochondrial oxidative stress and biosynthesis indices in carp liver

[0060]

[0061] Note: TFAM, mitochondrial transcription factor A; mtDNA, mitochondrial DNA. Data in the same row with the same superscript letters are not significantly different. Otherwise, they are significantly different.

[0062] Table 7 Activity of carp liver mitochondrial complex

[0063]

[0064] Note: Data in the same industry with the same superscript letters have no significant difference. Otherwise, there is a significant difference.

[0065] As shown in Table 5, after 12 weeks of breeding experiments, the weight gain rate of carp in the high sugar group was significantly lower than that in the control group (P<0.05), while the liver-to-body ratio, blood sugar level, glycosylated serum protein, glycation end products, insulin, triglycerides and cholesterol in the high sugar group were significantly increased (P<0.05), indicating that long-term feeding of high sugar feed can lead to liver enlargement, enhanced lipid synthesis ability, increased blood sugar and reduced blood sugar regulation ability. However, the biochemical indicators of the high sugar + regulatory composition group were significantly better than those of the high sugar group (P<0.05), and its blood sugar clearance ability was also significantly improved (P<0.05), and there was no significant difference compared with the control group (P>0.05). In addition, adding 1.0% regulatory composition can significantly increase plasma insulin levels (P<0.05). This shows that adding the sugar metabolism regulating composition prepared by Example 2 to high-sugar feed can improve the growth performance of high-sugar stressed carp to a certain extent, significantly reduce its blood sugar level, improve the body's blood sugar clearance ability, promote insulin secretion, and reduce metabolic disorders caused by high-sugar feed, thereby achieving the purpose of improving the economic benefits of aquaculture.

[0066] As shown in Tables 6 and 7, the liver mitochondrial MDA content in the high glucose group was significantly higher than that in the control group, but the CAT activity and TFAM and mtDNA expression levels were opposite (P<0.05). The high glucose + regulatory composition group reduced the liver mitochondrial MDA content, but the CAT activity and TFAM and mtDNA expression levels were significantly higher than those in the high glucose group (P<0.05). In addition, compared with the control group, the high glucose group significantly reduced the activity of mitochondrial complexes I, II and V, while the addition of the regulatory composition significantly improved the above indicators. The above results show that the high glucose group significantly increased the level of mitochondrial oxidative stress, but reduced mitochondrial biogenesis and mitochondrial function, while the addition of the regulatory composition can significantly reduce mitochondrial oxidative stress, increase the level of mitochondrial biogenesis, and ultimately improve mitochondrial function.

[0067] Depend on Figure 7It can be seen that after the glucose load test, blood glucose levels increased significantly in terms of sampling time, with a maximum blood glucose level at 1 hour. Subsequently, blood glucose decreased significantly and returned to the baseline value at 8 hours. In terms of diet composition, the maximum blood glucose level in the high sugar + regulated combination group was significantly lower than that in the other groups (P < 0.05). In addition, the interaction between sampling time and diet composition significantly affected blood glucose levels (P < 0.01). Between 1 and 8 hours, the blood glucose level in the high sugar + regulated combination group was significantly lower than that in the other groups (P < 0.05).

[0068] Test Example 3 Channel Catfish Culture Test and Result Analysis

[0069] The breeding experiment was carried out at the Aquatic Teaching and Research Base of Nanjing Agricultural University (Xingdian Town, Pukou District, Nanjing). The spotted catfish juveniles used were purchased from the National Aquatic Breeding Farm in Yangzhou City, Jiangsu Province. The experiment started from early July to mid-September, with a period of 8 weeks. 192 spotted catfish juveniles weighing 25.87±0.38g were randomly placed into 12 cages (specifications: 2.0m×1.0m×1.0m), with 16 fish in each cage. Three treatment groups were set up, namely 1) the control group, which was fed with a basic feed (feed sugar level of 33%); 2) the high sugar group, which was fed with a high sugar feed (feed sugar level of 45%); 3) the high sugar + regulation composition group, which was fed with a high sugar feed + 1.0% regulation composition prepared in Example 3. The feed formula components are shown in Table 8. After the breeding experiment, blood and liver samples were collected for index determination.

[0070] Table 8 Test feed formula

[0071]

[0072] Table 9 Growth performance and blood biochemical indicators of channel catfish

[0073]

[0074] Note: Data in the same industry with the same superscript letters have no significant difference. Otherwise, there is a significant difference.

[0075] Table 10 Mitochondrial oxidative stress and biosynthesis indices in channel catfish liver

[0076]

[0077] Note: TFAM, mitochondrial transcription factor A; mtDNA, mitochondrial DNA. Data in the same row with the same superscript letters are not significantly different. Otherwise, they are significantly different.

[0078] Table 11 Activity of mitochondrial complex in channel catfish liver

[0079]

[0080] Note: Data in the same industry with the same superscript letters have no significant difference. Otherwise, there is a significant difference.

[0081] As shown in Table 9, after 8 weeks of breeding experiments, the weight gain rate of channel catfish in the high sugar group was significantly higher than that in the control group (P < 0.05), but the liver-to-body ratio, blood sugar level, glycosylated serum protein, advanced glycation end products, insulin and cholesterol in the high sugar group were also significantly increased (P < 0.05), indicating that long-term feeding of high sugar feed can lead to its liver enlargement, enhanced lipid synthesis ability, increased blood sugar and reduced blood sugar regulation ability. However, the high sugar + regulation composition group further significantly improved the weight gain rate and insulin level, and biochemical indicators were significantly better than those in the high sugar group (P < 0.05). This shows that adding the sugar metabolism regulation composition prepared by Example 3 in high sugar feed can improve the growth performance of carp under high sugar stress to a large extent, significantly reduce its blood sugar level, improve the body's blood sugar clearance ability, promote insulin secretion, reduce the metabolic disorder caused by high sugar feed, and ultimately improve the economic benefits of aquaculture.

[0082] As shown in Tables 10 and 11, the liver mitochondrial MDA content in the high glucose group was significantly higher than that in the control group, but the CAT activity and TFAM and mtDNA expression levels were opposite (P<0.05). The high glucose + regulatory composition group reduced the liver mitochondrial MDA content, but the CAT activity and TFAM and mtDNA expression levels were significantly higher than those in the high glucose group (P<0.05). In addition, compared with the control group, the high glucose group significantly reduced the activity of mitochondrial complexes III and V, while the addition of the regulatory composition significantly improved the above indicators. The above results show that the high glucose group significantly increased the level of mitochondrial oxidative stress, but reduced mitochondrial biogenesis and mitochondrial function, and the addition of the regulatory composition can significantly reduce mitochondrial oxidative stress, increase the level of mitochondrial biogenesis, and ultimately improve mitochondrial function.

Claims

1. A mitochondrial nutrient composition for improving sugar metabolism as a high-sugar feed additive for fish, characterized in that: The nutrient composition comprises, by mass, 4-25 parts of honokiol, 2-20 parts of pyrroloquinoline quinone and 455-499 parts of sepiolite, and the mass ratio of the honokiol to the pyrroloquinoline quinone is 1.5-2.25:

1.

2. The mitochondrial nutrient composition for improving glucose metabolism according to claim 1, characterized in that The nutrient composition comprises 13-22 parts of honokiol, 4-20 parts of pyrroloquinoline quinone and 455-483 parts of sepiolite in parts by mass.

3. The mitochondrial nutrient composition for improving glucose metabolism according to claim 1, characterized in that The nutrient composition comprises, by weight, 15-20 parts of honokiol, 10-20 parts of pyrroloquinoline quinone and 460-470 parts of sepiolite.

4. A method for preparing the mitochondrial nutrient composition for improving glucose metabolism according to claim 1, characterized in that: The following steps are involved: (1) Preparation of pyrroloquinoline quinone; (2) Preparation of magnolol; (3) Screening of sepiolite; (4) Evenly mix the pyrroloquinoline quinone, magnolol and sieved sepiolite to obtain a nutrient composition.

5. The preparation method according to claim 4, characterized in that The pyrroloquinoline quinone is prepared by a microbial fermentation method, and the original bacteria are methylotrophic bacteria.

6. The preparation method according to claim 4, characterized in that The honokiol is prepared by ethanol extraction, and the raw material for extraction is the traditional Chinese medicine magnolia bark.

7. The preparation method according to claim 4, characterized in that The sepiolite was sieved through a 60-mesh sieve.

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