Composition containing dandelion and pericarpium citri reticulatae extracts and preparation method thereof

Abstract

The invention relates to a composition containing dandelion and pericarpium citri reticulatae extracts and a preparation method of the composition, and belongs to the technical field of health-care food. The traditional Chinese medicine composition is prepared from a dandelion extract, a pericarpium citri reticulatae extract, a semen coicis extract, a folium cortex eucommiae extract, a poria cocos extract, a fig extract, pyrroloquinoline quinone and a vitamin B. In the preparation process of the dandelion extract, ultrasonic assistance is utilized, dissolution is accelerated, meanwhile, the quality of the extract is improved through regulation and control of ultrasonic frequency, and the quality of the extract is improved. In the preparation process of the pericarpium citri reticulatae extract, an enzymolysis process is utilized to optimize the composition and proportion of active ingredients, and the composition provided by the invention conforms to the concept of homology of medicine and food and can meet the expectation of consumers on natural, efficient, green and safe health food.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of health food, and particularly relates to a composition containing dandelion and dried orange peel extracts and a preparation method thereof. BACKGROUND

[0002] Health food is a kind of special food with the main purpose of regulating human physiological functions and maintaining the health of the body, which is between medicine and ordinary food. Compared with medicine, health food focuses on prevention and conditioning, and has the advantages of high safety, small side effects, and convenient eating; compared with ordinary food, health food also has certain functionality and nutritional value. With the acceleration of social rhythm, the increase of life pressure and the universalization of sub-health problems, people's health consciousness is constantly improving, and health food has gradually become an important means of modern people's daily health management.

[0003] However, although there are various health foods on the market at present, covering multiple aspects such as enhancing immunity, regulating intestinal function, and improving metabolic state, there are still many deficiencies as a whole. For example, some products, in pursuit of significant short-term efficacy, often rely excessively on chemical synthetic ingredients, leading to doubts about safety and long-term consumption; the efficacy of some products is far from the actual effect, and it is difficult to meet the expectations of consumers for natural, efficient, green and safe health food. Based on this, the concept of medicinal and edible food has become an important research and development direction of health food. Medicinal and edible food materials are derived from animals and plants, and their ingredients are natural, with both edible and conditioning values, which can promote health while avoiding adverse reactions and are suitable for most people to eat. However, due to the relatively limited function of single medicinal and edible food materials, their conditioning effect usually cannot meet multiple needs such as clearing heat, reducing edema, and slimming, so technical improvement is urgently needed. SUMMARY

[0004] The present application aims to overcome the deficiencies of the prior art and provide a composition containing dandelion and dried orange peel extracts and a preparation method thereof.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows: A composition containing dandelion and dried orange peel extracts, the composition comprising the following components: dandelion extract, dried orange peel extract, yiyi extract, eucommia leaf extract, poria cocos extract, fig extract, pyrroloquinoline quinone, vitamin B1, vitamin B2 and vitamin B6.

[0006] Coix seed extract is derived from the seeds of coix, rich in various active ingredients, studies have shown that its heat-clearing and spleen-strengthening effects are related to the inhibition of inflammatory factor release, in the body slimming aspect, it can regulate adipocyte differentiation and promote fatty acid oxidation process, thereby helping to reduce body fat accumulation, at the same time, coix seed extract can affect the sodium ion reabsorption of the kidney, relieve abnormal water retention, and play a diuresis effect.

[0007] Eucommia leaf extract is derived from the leaves of eucommia tree, rich in active ingredients such as chlorogenic acid, has a relatively clear biological regulation effect, it can help cells more effectively process and expel oxidative byproducts and accumulations produced during metabolism, showing heat-clearing and detoxifying effects, at the same time, it also makes it more difficult for water retention between tissues to occur, and the swelling is relieved, improving the digestive problems caused by excessive humidity and weak spleen and stomach, and promoting lipid-lowering effect.

[0008] The main active ingredients of Poria cocos extract are polysaccharides and terpenoids, its mechanism of action in the body is closely related to protein regulation, especially the functional regulation of aquaporin, not only promotes the transport and excretion of excess water to relieve edema, but also controls the body fluid balance and reduces inflammation, achieving better physiological circulation.

[0009] Fig extract has the characteristics of mild nourishment, can well harmonize the body function, help to remove heat evil in the body, play the effect of clearing heat and detoxifying, adapt to other natural extracts, help to improve the problem of edema, harmonize the state of spleen and stomach, and assist in reducing fat accumulation.

[0010] According to some embodiments of the present application, the dandelion extract is prepared by the following steps: Wash the dandelion, dry, crush, sieve, get the dandelion material to be extracted, add deionized water according to the mass ratio of 1:20-25, ultrasonic assisted extraction, centrifugal, collect the supernatant, filter, concentrate, spray dry.

[0011] Dandelion extract is derived from dandelion herb, rich in flavonoids, polysaccharides and other active ingredients, is a common natural health raw material, which takes water metabolism as the core, by accelerating the discharge of waste and excess water, not only can remove internal heat, relieve dry heat and other related discomforts, indirectly play the effect of spleen-strengthening and transportation, but also can improve the edema, slimming and lipid-lowering, the present application selects ultrasonic assisted extraction during extraction, accelerates the dissolution, at the same time, through the regulation of ultrasonic frequency, improves the quality of the extract.

[0012] Further, the ultrasonic assisted extraction refers to heating to 50-70℃ and keeping warm for 3h, the whole process is carried out simultaneously with ultrasonic, the fixed power is 400W, first treated for 10min at a frequency of 10-15kHz, then treated for 20min at a frequency of 30-40kHz, and the cycle operation is continued until the end.

[0013] Further, the concentration refers to concentration to a solid content of 15-20 wt%, by means of vacuum concentration at a vacuum degree of -0.095 MPa at 40-50 DEG C.

[0014] According to some embodiments of the present application, the pericarpium citri reticulatae extract is prepared by the following steps: The pericarpium citri reticulatae is washed, dried, crushed, sieved, to obtain pericarpium citri reticulatae material to be extracted, deionized water is added at a mass ratio of 1:15-20, heated and kept warm, cellulase and hemicellulase are added, once enzymolysis, papain is added, twice enzymolysis, enzyme inactivation, centrifugation, collection of supernatant, filtration, concentration, spray drying.

[0015] The pericarpium citri reticulatae extract is derived from the outer skin of citrus fruits and rich in flavonoid active ingredients. As a traditional medicinal and edible material, it has the main effect of invigorating the spleen and tonifying qi in traditional Chinese medicine theory, which helps to enhance the transportation capacity of the spleen and stomach to nutrient substances, thereby achieving various health care purposes. With the improvement of the function of the spleen and stomach, the internal heat problem caused by poor metabolism can be effectively alleviated, and the effect of clearing heat and resolving toxins can be played. At the same time, it is helpful for the reasonable distribution of nutrients and water, preventing edema caused by poor metabolism, and reducing the accumulation of waste in the body. In the extraction process of the present application, enzyme hydrolysis process is used to optimize the composition and ratio of active ingredients.

[0016] Further, the heating and keeping warm refers to heating to 42-58 DEG C and keeping warm.

[0017] Further, the mass ratio of the pericarpium citri reticulatae material to be extracted, the cellulase, the hemicellulase and the papain is 100:0.1-0.15:0.05-0.1:0.05.

[0018] Further, the enzyme activity of the cellulase is 10000-15000 U / g; the enzyme activity of the hemicellulase is 10000 U / g; and the enzyme activity of the papain is 6000-10000 U / g.

[0019] Further, the time of the first enzymolysis is 30-40 min.

[0020] Further, the time of the second enzymolysis is 60-90 min.

[0021] Further, the concentration refers to concentration to a solid content of 15-20 wt%, by means of vacuum concentration at a vacuum degree of -0.095 MPa at 40-50 DEG C.

[0022] According to some embodiments of the present invention, the mass ratio of the dandelion extract, the tangerine peel extract, the coix seed extract, the eucommia leaf extract, the poria extract, the fig extract, the pyrrolizinone, the vitamin B1, the vitamin B2, and the vitamin B6 is 32-40:28-35:8-14:5-7:10-13:15:0.4:1-1.4:1:0.5-1.2.

[0023] According to some embodiments of the present invention, the composition is consumed by diluting it with water at a ratio of 1-1.5g:200-250mL once a day.

[0024] A method for preparing a composition containing dandelion and tangerine peel extracts, the preparation method comprising the following steps: The following ingredients were mixed: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2 and vitamin B6.

[0025] The beneficial effects of this invention are as follows: (1) This invention selects dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract and other ingredients to form a compound based on the mechanisms of action such as metabolism, inflammation inhibition and biological (functional) regulation, and provides a composition with the effects of clearing heat, reducing edema, strengthening the spleen and removing dampness and slimming.

[0026] (2) The composition provided by the present invention uses natural extracts as the core components, which conforms to the concept of food and medicine from the same source, is suitable for most people to consume, and has few adverse reactions.

[0027] (3) The composition provided by the present invention can meet consumers’ expectations for natural, efficient, green and safe health food. Detailed Implementation

[0028] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0029] Some of the raw materials used in the subsequent embodiments were obtained through commercial purchases, as detailed below: Dandelion, Bozhou Wufang Jiuru Pharmaceutical Co., Ltd.; Guangchenpi (dried tangerine peel), Bozhou Renyi Chinese Medicinal Herbs Sales Co., Ltd.; Coix seed extract, Eucommia leaf extract, Poria cocos extract, and fig extract, manufactured by Shaanxi Junhe Biotechnology Co., Ltd., all have a 10:1 ratio, are extracted by water extraction, and are all food grade.

[0030] Example 1 A composition containing dandelion and tangerine peel extracts, the composition comprising the following components: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2, and vitamin B6, wherein the mass ratio is 40:35:14:7:13:15:0.4:1.4:1:1.2; the composition is to be consumed by diluting it with water at room temperature at a ratio of 1.5g:250mL once a day.

[0031] The dandelion extract is prepared through the following steps: Dandelion was washed with deionized water, dried at 42℃ to a moisture content of 2.6wt%, pulverized, and passed through a 0.5mm sieve to obtain the dandelion material to be extracted. Deionized water was added at a mass material-to-water ratio of 1:25, and ultrasonic extraction was performed (specifically, the temperature was raised to 70℃ and held for 3 hours, with a heating rate of 3℃ / min, and ultrasonication was performed simultaneously throughout the process, with a fixed power of 400W, first treated at a frequency of 15kHz for 10 minutes, then treated at a frequency of 40kHz for 20 minutes, and the operation was repeated until the end). The mixture was centrifuged (10000r / min, 10 minutes), the supernatant was collected, the insoluble matter was removed by filtration, and the mixture was concentrated to a solid content of 20wt% by vacuum concentration at 50℃ under a vacuum of -0.095MPa. The mixture was then spray-dried (inlet air temperature 180℃, outlet air temperature 80℃).

[0032] The tangerine peel extract is prepared through the following steps: The dried tangerine peel was washed with deionized water, dried at 40℃ to a moisture content of 2.3wt%, pulverized, and passed through a 0.5mm sieve to obtain the material to be extracted. Deionized water was added at a mass material-to-water ratio of 1:20, and the temperature was raised to 58℃ and maintained at a rate of 3℃ / min. Cellulase (15000U / g) and hemicellulase (10000U / g) were added, and the enzymatic hydrolysis was carried out for 40 minutes. Papain (10000U / g) was added, and the enzymatic hydrolysis was carried out for 90 minutes. The enzyme was inactivated by treatment at 95℃ for 5 min, centrifuged (10000 r / min, 10 min), the supernatant was collected, the insoluble matter was removed by filtration, and the concentration was made up to a solid content of 20 wt%. The concentration was carried out under reduced pressure at 50℃ with a vacuum degree of -0.095 MPa, followed by spray drying (inlet air temperature 180℃, outlet air temperature 80℃). The mass ratio of the tangerine peel material to be extracted, the cellulase, the hemicellulase and the papain was 100:0.15:0.1:0.05.

[0033] A method for preparing a composition containing dandelion and tangerine peel extracts, the preparation method comprising the following steps: The following ingredients were mixed: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2 and vitamin B6.

[0034] Example 2 A composition containing dandelion and tangerine peel extracts, the composition comprising the following components: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2, and vitamin B6, wherein the mass ratio is 38:30:12:6:11:15:0.4:1.2:1:0.8; the composition is to be consumed by diluting it with water at room temperature at a ratio of 1.2g:230mL once a day.

[0035] The dandelion extract is prepared through the following steps: Dandelion was washed with deionized water, dried at 40℃ to a moisture content of 1.3wt%, pulverized, and passed through a 0.5mm sieve to obtain the dandelion material to be extracted. Deionized water was added at a mass material-to-water ratio of 1:21, and ultrasonic extraction was performed (specifically, the temperature was raised to 60℃ and held for 3 hours, with a heating rate of 3℃ / min, and ultrasonication was performed simultaneously throughout the process, with a fixed power of 400W, first treated at a frequency of 12kHz for 10 minutes, then treated at a frequency of 35kHz for 20 minutes, and the operation was repeated until the end). The mixture was centrifuged (10000r / min, 10 minutes), the supernatant was collected, the insoluble matter was removed by filtration, and the mixture was concentrated to a solid content of 18wt% by vacuum concentration at 45℃ under a vacuum of -0.095MPa. The mixture was then spray-dried (inlet air temperature 180℃, outlet air temperature 80℃).

[0036] The tangerine peel extract is prepared through the following steps: The dried tangerine peel was washed with deionized water, dried at 45℃ to a moisture content of 2.1wt%, pulverized, and passed through a 0.5mm sieve to obtain the material to be extracted. Deionized water was added at a mass material-to-water ratio of 1:18, and the temperature was raised to 55℃ and maintained at a rate of 3℃ / min. Cellulase (12000U / g) and hemicellulase (10000U / g) were added, and the first enzymatic hydrolysis was carried out for 35 minutes. Papain (8000U / g) was added, and the second enzymatic hydrolysis was carried out for 75 minutes. The enzyme was inactivated by treatment at 95℃ for 5 min, followed by centrifugation (10000 r / min, 10 min), collection of supernatant, filtration to remove insoluble matter, and concentration to a solid content of 17 wt%. The concentration was carried out under reduced pressure at 43℃ under a vacuum of -0.095 MPa, followed by spray drying (inlet air temperature 180℃, outlet air temperature 80℃). The mass ratio of the tangerine peel material to be extracted, the cellulase, the hemicellulase, and the papain was 100:0.13:0.07:0.05.

[0037] A method for preparing a composition containing dandelion and tangerine peel extracts, the preparation method comprising the following steps: The following ingredients were mixed: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2 and vitamin B6.

[0038] Example 3 A composition containing dandelion and tangerine peel extracts, the composition comprising the following components: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2, and vitamin B6, wherein the mass ratio is 32:28:8:5:10:15:0.4:1:1:0.5; the composition is to be consumed by diluting it with water at room temperature at a ratio of 1g:200mL once a day.

[0039] The dandelion extract is prepared through the following steps: Dandelion was washed with deionized water, dried at 45℃ to a moisture content of 1.8wt%, pulverized, and passed through a 0.5mm sieve to obtain the dandelion material to be extracted. Deionized water was added at a mass material-to-water ratio of 1:20, and ultrasonic extraction was performed (specifically, the temperature was raised to 50℃ and held for 3 hours, with a heating rate of 3℃ / min, and ultrasonication was performed simultaneously throughout the process, with a fixed power of 400W, first treated at a frequency of 10kHz for 10 minutes, then treated at a frequency of 30kHz for 20 minutes, and the operation was repeated until the end). The mixture was centrifuged (10000r / min, 10 minutes), the supernatant was collected, the insoluble matter was removed by filtration, and the mixture was concentrated to a solid content of 15wt% by vacuum concentration at 40℃ under a vacuum of -0.095MPa. The mixture was then spray-dried (inlet air temperature 180℃, outlet air temperature 80℃).

[0040] The tangerine peel extract is prepared through the following steps: The dried tangerine peel was washed with deionized water, dried at 45℃ to a moisture content of 2.5wt%, pulverized, and passed through a 0.5mm sieve to obtain the material to be extracted. Deionized water was added at a mass material-to-water ratio of 1:15, and the temperature was raised to 42℃ and maintained at a rate of 3℃ / min. Cellulase (10000U / g) and hemicellulase (10000U / g) were added, and the enzymatic hydrolysis was carried out for 30 minutes. Papain (6000U / g) was added, and the enzymatic hydrolysis was carried out for 60 minutes. The enzyme was inactivated by treatment at 95℃ for 5 min, followed by centrifugation (10000 r / min, 10 min), collection of supernatant, filtration to remove insoluble matter, and concentration to a solid content of 15 wt%. The concentration was carried out under reduced pressure at 40℃ under a vacuum of -0.095 MPa, followed by spray drying (inlet air temperature 180℃, outlet air temperature 80℃). The mass ratio of the tangerine peel material to be extracted, the cellulase, the hemicellulase, and the papain was 100:0.1:0.05:0.05.

[0041] A method for preparing a composition containing dandelion and tangerine peel extracts, the preparation method comprising the following steps: The following ingredients were mixed: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2 and vitamin B6.

[0042] Comparative Example 1 Compared with Example 2, the composition in Comparative Example 1 does not contain dandelion extract, and the missing weight is made up with tangerine peel extract, while the rest of the operations, steps and parameters remain unchanged.

[0043] Comparative Example 2 Compared with Example 2, the difference is that the composition in Comparative Example 2 does not contain tangerine peel extract, and the missing weight is made up with dandelion extract, while the rest of the operations, steps and parameters remain unchanged.

[0044] Comparative Example 3 Compared with Example 2, the difference is that the composition in Comparative Example 3 does not contain coix seed extract, and the missing weight is made up with Eucommia ulmoides leaf extract, Poria cocos extract and fig extract in a mass ratio of 6:11:15. All other operations, steps and parameters remain unchanged.

[0045] Comparative Example 4 Compared with Example 2, the difference is that the composition in Comparative Example 4 does not contain Eucommia ulmoides leaf extract, and the missing weight is made up with Coix seed extract, Poria cocos extract and fig extract in a mass ratio of 12:11:15. All other operations, steps and parameters remain unchanged.

[0046] Comparative Example 5 Compared with Example 2, the difference is that the composition in Comparative Example 5 does not contain Poria cocos extract, and the missing weight is made up with Coix seed extract, Eucommia ulmoides leaf extract and fig extract in a mass ratio of 12:6:15. All other operations, steps and parameters remain unchanged.

[0047] Comparative Example 6 Compared with Example 2, the difference is that the composition in Comparative Example 6 does not contain fig extract, and the missing weight is made up with coix seed extract, eucommia leaf extract and poria extract in a mass ratio of 12:6:11. All other operations, steps and parameters remain unchanged.

[0048] Comparative Example 7 Compared with Example 2, the difference is that "processing at 12kHz for 10 minutes first" in Example 2 is changed to "processing at 35kHz for 10 minutes first". In short, Comparative Example 7 always uses assisted extraction at 35kHz, and the other operations, steps and parameters remain unchanged.

[0049] Comparative Example 8 Compared with Example 2, the difference is that "then process at a frequency of 35kHz for 20 minutes" in Example 2 is changed to "then process at a frequency of 12kHz for 20 minutes". In short, Comparative Example 8 always uses assisted extraction at a frequency of 12kHz, and the other operations, steps and parameters remain unchanged.

[0050] Comparative Example 9 Compared with Example 2, the difference is that the "add cellulase (12000U / g) and hemicellulase (10000U / g), first enzymatic hydrolysis for 35 min, add papain (8000U / g), second enzymatic hydrolysis for 75 min" in Example 2 is changed to "add papain (8000U / g), first enzymatic hydrolysis for 35 min, add cellulase (12000U / g) and hemicellulase (10000U / g), second enzymatic hydrolysis for 75 min". All other operations, steps and parameters remain unchanged.

[0051] Comparative Example 10 Compared with Example 2, the difference is that the "add cellulase (12000U / g) and hemicellulase (10000U / g), hydrolyze for 35 min, add papain (8000U / g), hydrolyze for 75 min" in Example 2 is changed to "add cellulase (12000U / g), hemicellulase (10000U / g) and papain (8000U / g), hydrolyze for 110 min". All other operations, steps and parameters remain unchanged.

[0052] Test Example 1 Heat-clearing experiment: The steps include: 1) Label Example 2 and Comparative Examples 1-10 as Experimental Group, Control Group 1, Control Group 2, ..., and so on, corresponding to each comparative example; 2) Invite 180 volunteers who have started experiencing bad breath and yellow urine in the past 3 days, and who have not received any treatment or consumed any health food in the past 3 days. They are randomly divided into two groups of 15 people each, corresponding to the experimental group and control group mentioned above. One group will remain, which will be recorded as the blank group. 3) The experiment lasted for 7 days. During the 7 days, the blank group did not receive any special treatment. The other groups consumed the prepared composition daily. The method of consumption was to dilute the composition with water at room temperature at a ratio of 1.2g:230mL and consume it once a day. During the experiment, the participants maintained their previous living habits. After the experiment, the participants were judged according to the judgment criteria, and the corresponding number of participants was recorded. 4) Judgment criteria: The symptoms of halitosis and yellow urine have basically disappeared, indicating a cure; the symptoms of halitosis and yellow urine have significantly improved, indicating a significant improvement; the symptoms of halitosis and yellow urine have partially improved, indicating an effective condition; the symptoms of halitosis and yellow urine have no significant change, indicating an ineffective condition.

[0053] Table 1. Experimental results of Test Example 1 As shown in Table 1, when halitosis and yellow urine are used to reflect the heat-clearing effect, the composition prepared in this invention (experimental group) has the best experimental results, with 8 people cured and 7 people showing significant improvement.

[0054] Test Example 2 Slimming experiment: The steps include: 1) Label Example 2 and Comparative Examples 1-10 as Experimental Group, Control Group 1, Control Group 2, ..., and so on, corresponding to each comparative example; 2) A total of 180 male SPF-grade SD rats weighing 170-180g were randomly divided into two groups of 15 each, corresponding to the experimental group and the control group mentioned above. One group was left over and was designated as the blank group. 3) Rats in the experimental group, control group, and blank group were fed continuously for 8 weeks, during which they had free access to food. The diet consisted of 75 wt% basal feed, 15 wt% egg yolk powder, 5 wt% lard, 2 wt% white sugar, 2 wt% peanut and 1 wt% sesame oil. The basal feed consisted of 25 wt% wheat bran, 15 wt% fish meal, 15 wt% corn flour, 20 wt% soybean flour, 10 wt% dehydrated cabbage, 10 wt% barley flour, 1 wt% salt, 3 wt% corn oil and 1 wt% whey powder. The blank group did not receive any special treatment. The other groups were also given the prepared composition by gavage once a day at a dose of 400 mg / kg. After the experiment, the rats were fasted and deprived of water for 12 hours, weighed, euthanized, and the adipose tissue around the kidneys was weighed. 4) Calculate the fat-body ratio of the rats using the formula: fat weight (perirenal area) / body weight. Convert the result to a percentage value and then calculate the average value for each group.

[0055] Table 2 shows the experimental results of Test Example 2. As shown in Table 2, when the fat-body ratio is used to reflect the slimming effect, the composition prepared in this invention (test group) has the best experimental results, with an average fat-body ratio of only 1.42%.

[0056] Test Example 3 Edema reduction experiment: The steps include: 1) Label Example 2 and Comparative Examples 1-10 as Experimental Group, Control Group 1, Control Group 2, ..., and so on, corresponding to each comparative example; 2) Invite volunteers who have morning edema in the past 30 days. The number of days with morning edema in the past 30 days must be 8-10 days (it will subside on its own and there are no other special discomforts). A total of 180 people will be invited and randomly divided into groups of 15 people each, corresponding to the experimental group and control group mentioned above. One group will be left over and recorded as the blank group. 3) The experiment lasted for 30 days. During the 30 days, the blank group did not receive any special treatment. The other groups consumed the prepared composition every other day. The method of consumption was to dilute the composition with water at room temperature at a ratio of 1.2g:230mL and consume it only once a day. During the experiment, the participants maintained their previous lifestyle. After the experiment, the participants were asked to give a self-score based on the improvement of morning edema during the experiment (compared with the 30 days before the experiment) and the average score of each group was calculated. 4) Scoring criteria: 1-10 points, with 1 point being the worst improvement and 10 points being the best improvement, progressing from 1 to 10. Only integer scores can be used for self-assessment.

[0057] Table 3 shows the experimental results of Test Example 3. As shown in Table 3, when the self-rating reflects the effect of reducing edema, the composition prepared in this invention (test group) has the best experimental results, with an average score of 8.9.

[0058] The above description is merely an example and illustration of the concept of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described or use similar methods to replace them, as long as they do not deviate from the concept of the invention or exceed the scope defined in the claims, they should all fall within the protection scope of the present invention.

Claims

1. A composition containing dandelion and tangerine peel extracts, characterized in that: The composition comprises the following components: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2 and vitamin B6.

2. The composition containing dandelion and tangerine peel extracts according to claim 1, characterized in that: The dandelion extract is prepared through the following steps: Wash dandelions, dry them, crush them, and sieve them to obtain the dandelion material to be extracted. Add deionized water at a mass material-to-water ratio of 1:20-25, perform ultrasonic-assisted extraction, centrifuge, collect the supernatant, filter, concentrate, and spray dry.

3. The composition containing dandelion and tangerine peel extracts according to claim 2, characterized in that: The ultrasound-assisted extraction refers to heating to 50-70℃ and holding for 3 hours, while simultaneously performing ultrasound at a fixed power of 400W. The process is first performed at a frequency of 10-15kHz for 10 minutes, and then at a frequency of 30-40kHz for 20 minutes, and the cycle is repeated until the end.

4. The composition containing dandelion and tangerine peel extracts according to claim 2, characterized in that: The concentration refers to concentrating to a solids content of 15-20 wt%.

5. The composition containing dandelion and tangerine peel extracts according to claim 1, characterized in that: The tangerine peel extract is prepared through the following steps: Clean the Guangchenpi (dried tangerine peel), dry it, crush it, and sieve it to obtain the Guangchenpi material to be extracted. Add deionized water at a mass material-to-water ratio of 1:15-20, heat it and keep it warm, add cellulase and hemicellulase for the first enzymatic hydrolysis, add papain for the second enzymatic hydrolysis, inactivate the enzymes, centrifuge, collect the supernatant, filter it, concentrate it, and spray dry it.

6. The composition containing dandelion and tangerine peel extract according to claim 5, characterized in that: The term "heating and then heat preservation" refers to maintaining the temperature after it has been raised to 42-58℃.

7. The composition containing dandelion and tangerine peel extract according to claim 5, characterized in that: The mass ratio of the tangerine peel material to be extracted, the cellulase, the hemicellulase, and the papain is 100:0.1-0.15:0.05-0.1:0.

05.

8. The composition containing dandelion and tangerine peel extracts according to claim 5, characterized in that: The time for the first enzymatic hydrolysis is 30-40 min; the time for the second enzymatic hydrolysis is 60-90 min.

9. The composition containing dandelion and tangerine peel extracts according to claim 1, characterized in that: The mass ratio of the dandelion extract, the tangerine peel extract, the coix seed extract, the eucommia leaf extract, the poria extract, the fig extract, the pyrroquinone, the vitamin B1, the vitamin B2, and the vitamin B6 is 32-40:28-35:8-14:5-7:10-13:15:0.4:1-1.4:1:0.5-1.

2.

10. A method for preparing a composition containing dandelion and tangerine peel extract according to any one of claims 1-9, characterized in that: The preparation method includes the following steps: The following ingredients were mixed: dandelion extract, tangerine peel extract, coix seed extract, eucommia leaf extract, poria cocos extract, fig extract, pyrrolquinone, vitamin B1, vitamin B2 and vitamin B6.

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