A Brachionus calyciflorus rotifer culture device

Abstract

The present invention belongs to the field of inventions, and discloses a Brachionus calyciflorus rotifer culture device, which can avoid the contamination of the rotifer bait culture environment by rotifer metabolites. The device comprises a device shell, a rotifer culture container sleeved inside the device shell, and an insect attracting light source. The rotifer culture container has a metabolite stop hole connected to a bait culture chamber, and the metabolite stop hole is used to stop the metabolites of the Brachionus calyciflorus rotifer from passing through. When implemented, a water body rich in rotifer bait is poured into the bait culture chamber, and the water level of the water body is made higher than the bottom of the rotifer culture container, so that the rotifer bait enters the rotifer culture container with the water flow through the metabolite stop hole, and then the Brachionus calyciflorus rotifer is cultured in the rotifer culture container, so that the rotifer can ingest the rotifer bait in the water body, and the metabolites of the rotifer are blocked in the rotifer culture container by the metabolite stop hole and will not enter the bait culture chamber.

Description

Technical Field

[0001] The invention belongs to the field of inventions, and in particular relates to a Brachionus calyciflorus rotifer cultivation device. Background Art

[0002] Brachionus calyciflorus lives mostly in freshwater areas. It feeds on single-celled microalgae and yeast by waving its head cilia and chewing with its chewing apparatus. It reproduces parthenogenetically most of the time in a year. It has high reproductive capacity and strong adaptability. It is widely used as an starter bait for fish, shrimp and crab larvae in freshwater aquaculture.

[0003] The body length of Brachionus calyciflorus rotifer is 100-500μm. When it is cultivated on a large scale, the phototaxis of rotifers can be used to gather rotifers by shining light on the water surface, and then harvested with a filter. Brachionus calyciflorus rotifers are often used as secondary bait organisms in aquaculture. They can obtain nutritional fortification from microalgae and other feeds to increase their own PUFA, vitamin and other content. In practical applications, the nutritionally fortified Brachionus calyciflorus rotifers can improve the survival rate of larvae such as fish, shrimp and crabs, and promote the growth and development of larvae. Chlorella is commonly used as feed, supplemented with single-cell yeast such as baker's yeast as an additive.

[0004] However, the existing rotifer culture still has the following problems that need to be solved urgently: in the process of cultivating Brachionus calyciflorus rotifers, a large amount of metabolites such as egg shells, corpses, and food residues will be produced, and these metabolites will affect the culture environment of rotifer bait. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides a Brachionus calyciflorus rotifer culture device, which can prevent the rotifer metabolites from polluting the rotifer bait culture environment.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A Brachionus calyciflorus rotifer culture device, characterized by comprising: a device housing, arranged vertically;

[0008] A rotifer culture container is sleeved inside the device shell, and a bait culture cavity is formed between the rotifer culture container and the device shell; and an insect-attracting light source is arranged near the upper end of the rotifer culture container, wherein the rotifer culture container has a metabolite stop hole connected to the bait culture cavity, and the metabolite stop hole is used to stop the metabolites of the Brachionus calyciflorus rotifer from passing through.

[0009] Preferably, the present invention further comprises an aeration pipe connected to an external air source, wherein the aeration pipe has an aeration port located at the bottom of a bait culture chamber, and the bait culture chamber is used to accommodate the bait of the Brachionus calyciflorus rotifer, wherein the bait of the Brachionus calyciflorus rotifer is green algae with a margin of notch.

[0010] Furthermore, the upper ends of the device shell and the rotifer culture container are both open, and there are four aeration pipes which are vertically and evenly arranged along the circumference of the device shell, and the aeration port is located below the bottom of the rotifer culture container.

[0011] Preferably, the present invention further comprises a combining frame which is detachably arranged on the upper end of the device housing, and the rotifer culture container is detachably arranged on the combining frame.

[0012] Furthermore, the insect-attracting light source is a light strip, which is arranged on the inner wall of the device shell along the circumference of the device shell, and the insect-attracting light source is located at the lower end of the combined frame and is spaced a predetermined distance from the combined frame.

[0013] Preferably, the bottom of the rotifer culture container isolates the bait culture cavity from the rotifer culture container, and the metabolite stop hole is formed on the peripheral surface of the rotifer culture container.

[0014] Furthermore, the bottom of the rotifer culture container is a circular plate, and the circumference of the rotifer culture container is formed by continuous enclosure of 300-mesh filter screens.

[0015] Preferably, the device housing is made of transparent material.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. The rotifer cultivation device of the Brachionus calyciflorus of the present invention comprises a device shell, a rotifer cultivation container sleeved inside the device shell, and an insect attracting light source, the rotifer cultivation container has a metabolite stop hole connected to the bait cultivation chamber, the metabolite stop hole is used to stop the metabolites of the Brachionus calyciflorus rotifer from passing through, during implementation, water rich in rotifer bait is poured into the bait cultivation chamber, and the water level of the water body is made higher than the bottom of the rotifer cultivation container, so that the rotifer bait enters the rotifer cultivation container with the water flow through the metabolite stop hole, and then the Brachionus calyciflorus rotifer is cultivated in the rotifer cultivation container, so that the rotifer can ingest the rotifer bait in the water, while the metabolites of the rotifer are blocked in the rotifer cultivation container by the metabolite stop hole and will not enter the bait cultivation chamber. Therefore, the present invention can avoid the pollution of the rotifer bait cultivation environment by the rotifer metabolites.

[0018] 2. Because the present invention also includes an aeration pipeline connected to an external air source, the aeration pipeline has an aeration port located at the bottom of the bait culture chamber, and the bait culture chamber is used to accommodate the bait of Brachionus calyciflorus rotifer, and the bait of Brachionus calyciflorus rotifer is notch edge green algae. The present invention uses notch edge green algae as a substitute bait for Chlorella plus additives. The cultivation practice of Brachionus calyciflorus rotifer shows that notch edge green algae can not only completely replace Chlorella plus additives, but also Brachionus calyciflorus rotifer fed with notch edge green algae has a higher population density and egg-carrying rate , and because of its extremely high arachidonic acid content, the rotifers cultured with Brachionus calyciflorus using the notched green algae can significantly promote the growth and development of fish larvae that use it as bait, but the notched green algae has a high sedimentation coefficient, and usually clusters and settle during the rotifer culture process, which is not conducive to the rotifers' planktonic feeding. Therefore, the present invention can further improve the nutritional value and breeding efficiency of the rotifers, and the notched green algae is always floated on the water surface through the aeration port, which is conducive to the rotifers' planktonic feeding.

[0019] 3. Because the present invention also includes a frame body detachably arranged on the upper end of the device shell, and the rotifer culture container is detachably arranged on the frame body, the component arrangement of the present invention is more flexible, which is more conducive to the maintenance, transportation and storage of the device.

[0020] 4. Because the bottom of the rotifer culture container of the present invention is a circular plate, the rotifer metabolites produced during the rotifer culture process are carried by the bottom of the rotifer culture container of the present invention.

[0021] 5. Because the housing of the device of the present invention is made of transparent material, the present invention can facilitate real-time monitoring of the cultivation process of Brachionus calyciflorus rotifer. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A schematic diagram of a rotifer culture device for Brachionus calyciflorus according to an embodiment of the present invention;

[0023] Figure 2 It is a top view of a Brachionus calyciflorus rotifer cultivation device according to an embodiment of the present invention.

[0024] In the figure: 100, Brachionus calyciflorus rotifer cultivation device, 1, device shell, 2, combined frame, 3, rotifer cultivation container, 31, container bottom, 32, container peripheral surface, 1a, bait cultivation chamber, 4, insect attractant light source, 5, aeration pipe. DETAILED DESCRIPTION

[0025] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the following examples and drawings specifically illustrate the rotifer cultivation device of Brachionus calyciflorus of the present invention. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0026] like Figure 1 and Figure 2 As shown, the Brachionus calyciflorus rotifer cultivation device 100 in this embodiment includes a device shell 1, a combination frame 2, a rotifer cultivation container 3, an insect attracting light source 4 and an aeration pipe 5.

[0027] The device housing 1 is made of a transparent material, is vertically arranged and has an open upper end. In the present embodiment, the device housing 1 is a cylindrical body with an open upper end.

[0028] The combining frame 2 is detachably disposed on the upper end of the device housing 1 .

[0029] The rotifer culture container 3 is sleeved inside the device shell 1, and the rotifer culture container 3 is detachably arranged on the combination frame 2. The upper end of the rotifer culture container 3 is open, and a bait culture chamber 1a is formed between the rotifer culture container 3 and the device shell 1. The bait culture chamber 1a is used to accommodate the bait of the calyx flower Brachionus rotifer, and the bait of the calyx flower Brachionus rotifer is notched-margin green algae. In the present embodiment, the rotifer culture container 3 is a cylindrical body with an open upper end, and the combination frame 2 has two threaded holes, one of which is arranged at the upper end of the device shell 1 through a threaded connection, and the other is arranged at the upper end of the rotifer culture container 3 through a threaded connection. The device shell 1 and the rotifer culture container 3 are coaxially arranged, and a certain distance is maintained between the inner wall of the device shell 1 and the outer surface of the rotifer culture container 3, so that the vertical section of the bait culture chamber 1a is "U"-shaped, that is, the volume space contour of the bait culture chamber 1a is a contour formed by rotating and lofting the "U"-shaped lofting section.

[0030] The rotifer culture container 3 has a metabolite stopping hole (not shown in the drawings) communicating with the bait culture chamber 1a, and the metabolite stopping hole is used to stop the metabolites of the Brachionus calyciflorus rotifer from passing through.

[0031] The bottom of the rotifer culture container 3 is a circular plate, which serves as the container bottom 31. The container bottom 31 isolates the bait culture chamber 1a from the rotifer culture container 3. The circumference of the rotifer culture container 3 serves as the container circumference 32. The metabolite stop hole is formed on the container circumference 32. Specifically, the circumference of the rotifer culture container is formed by continuously enclosing a 300-mesh filter screen, and the container bottom 31 and the container circumference 32 are detachably arranged.

[0032] The insect attracting light source 4 is arranged near the upper end of the rotifer culture container 3. Specifically, the insect attracting light source 4 is a light strip, which is arranged on the inner wall of the device shell 1 along the circumference of the device shell 1, and the insect attracting light source 4 is located at the lower end of the combining frame 2, and is separated from the combining frame 2 by a predetermined distance. In the present embodiment, the insect attracting light source 4 is a circular ring light source.

[0033] The aeration pipe 4 is connected to an external air source (not marked in the drawings), and has an aeration port (not marked in the drawings) located at the bottom of the bait culture chamber 1a. Specifically, there are four aeration pipes 4, which are vertically and evenly arranged along the circumference of the device shell 1, and the aeration port is located below the bottom of the rotifer culture container 3.

[0034] The following is a description of the use of the Brachionus calyciflorus rotifer cultivation device 100 in conjunction with an embodiment:

[0035] First, an algae liquid rich in the notched-margin green algae species is poured into the rotifer culture container 3, and the liquid level of the algae liquid is higher than the bottom of the container, that is, the algae liquid enters the rotifer culture container 3 through the metabolite stop hole, and then, the rotifer species of Brachionus calyciflorus is poured into the rotifer culture container 3, and the liquid level of the algae liquid after pouring is below the lower edge of the insect attracting light source 4, and then, the external air source is started, and the bottom of the algae liquid is aerated through the aeration pipe 5, so that the notched-margin green algae can be kept in a suspended state, so as to facilitate the supply of The rotifer Brachionus calyciflorus is planktonic and then, after a period of rotifer cultivation, the external air source is turned off, and the high sedimentation coefficient of the notched green algae is utilized to allow the metabolites produced by the notched green algae and the rotifer Brachionus calyciflorus during the cultivation process to settle on the bottom 31 of the container. Finally, the insect attracting light source 4 is turned on in a dark environment, and the phototaxis of the rotifer Brachionus calyciflorus is utilized to gather the living rotifer Brachionus calyciflorus on the surface of the algae liquid, and the gathered Brachionus calyciflorus rotifers are collected in a centralized manner.

[0036] In addition, after the use of the Brachionus calyciflorus rotifer culture device 100 is completed, the combined frame 2 is removed, the container bottom 31 and the container peripheral surface 32 are disassembled, and they are stored for future use after cleaning and disinfection, while the device shell 1 can be used to continue to culture the notched green algae.

[0037] The above-mentioned implementation modes are preferred cases of the present invention and are not used to limit the protection scope of the present invention. Various deformations or modifications that can be made by ordinary technicians in this field without creative work within the scope of the attached claims are still within the protection scope of this patent.

Claims

1. A rotifer culture device for Brachionus calyciflorus, characterized in that: include: The device housing is arranged vertically; A rotifer culture container is sleeved inside the device housing, and a bait culture chamber is formed between the rotifer culture container and the device housing; and The insect attracting light source is arranged near the upper end of the rotifer culture container. Wherein, the rotifer culture container has a metabolite stop hole connected to the bait culture chamber, and the metabolite stop hole is used to stop the metabolites of Brachionus calyciflorus from passing through. The Brachionus calyciflorus rotifer culture device also includes a combination frame, which is detachably arranged on the upper end of the device shell, and the rotifer culture container is detachably arranged on the combination frame.

2. The Brachionus calyciflorus rotifer culture device according to claim 1, characterized in that: Also includes: an aeration pipe connected to an external air source, wherein the aeration pipe has an aeration port located at the bottom of the bait culture chamber, The bait culture chamber is used to contain the bait of Brachionus calyciflorus rotifer, and the bait of Brachionus calyciflorus rotifer is green algae with notched margin.

3. The Brachionus calyciflorus rotifer culture device according to claim 2, characterized in that: in, The upper ends of the device housing and the rotifer culture container are both open. The number of the aeration pipes is four, which are vertically and evenly arranged along the circumference of the device shell, and the aeration port is located below the bottom of the rotifer culture container.

4. The Brachionus calyciflorus rotifer culture device according to claim 1, characterized in that: in, The insect attracting light source is a light strip, which is arranged on the inner wall of the device shell along the circumference of the device shell, and the insect attracting light source is located at the lower end of the combination frame and is spaced a predetermined distance from the combination frame.

5. The Brachionus calyciflorus rotifer culture device according to claim 1, characterized in that: in, The bottom of the rotifer culture container isolates the bait culture cavity from the rotifer culture container, and the metabolite stop hole is formed on the peripheral surface of the rotifer culture container.

6. The Brachionus calyciflorus rotifer culture device according to claim 5, characterized in that: in, The bottom of the rotifer culture container is a circular plate, and the peripheral surface of the rotifer culture container is formed by continuous enclosure of 300-mesh filter screens.

7. The Brachionus calyciflorus rotifer culture device according to claim 1, characterized in that: in, The device shell is made of transparent material.

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