Betaine Emulsion Feed Additive, Its Preparation Method and Application

Abstract

The present invention provides a betaine emulsion feed additive, a preparation method thereof and an application. The betaine emulsion feed additive comprises: a betaine solution, soybean oil and an emulsifier, wherein the content of betaine in the betaine solution is 30 wt% to 50 wt%. In this application, a betaine solution with a specific concentration is mixed with oil and an emulsifier to obtain a feed additive in the form of an emulsion. This emulsion feed additive can be added to aquatic expanded feeds by spraying during the feed processing, and can replace the process of spraying oil into expanded feeds during the production of aquatic feeds. The betaine emulsion feed additive can be added to feeds in a manner similar to that of oil, is convenient to use, is easily dispersed evenly, is beneficial to the absorption of betaine by the animals being fed, and in particular, can improve the feeding of aquatic animals, reduce the feed coefficient, accelerate fat metabolism and reduce the accumulation of liver fat for aquatic animals.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture or aquatic feed, and in particular, to a betaine emulsion feed additive, a preparation method thereof, and an application thereof. Background Art

[0002] Betaine, namely trimethylglycine, widely exists in animals and plants in nature. It is named because it was initially extracted from beet molasses and has the functions of anti-tumor, blood pressure lowering, anti-peptic ulcer and gastrointestinal dysfunction, and treating liver lesions.

[0003] During the aquaculture process of aquatic animals, adding it to the feed can provide methyl donors, reduce the use of part of methionine, not only can regulate the internal acid-base environment, reduce stress, promote lipid and protein metabolism, improve the lean meat rate, but also has a good feeding effect in aquatic feed.

[0004] Betaine has strong hygroscopic properties and is prone to caking and moisture absorption during use, which to a certain extent limits its use in aquatic feed. Summary of the Invention

[0005] The main object of the present invention is to provide a betaine emulsion feed additive, a preparation method thereof, and an application thereof to solve the problems of easy moisture absorption and caking of betaine during use in the prior art.

[0006] To achieve the above object, according to one aspect of the present invention, there is provided a betaine emulsion feed additive, which includes: a betaine solution, soybean oil and an emulsifier, and the content of betaine in the betaine solution is 30wt% - 50wt%.

[0007] Further, the weight ratio of the betaine solution to the oil is 1:1 - 10, and the emulsifier is 1wt‰ - 10wt‰ of the total amount of the additive.

[0008] Further, the betaine solution is a natural betaine extract, and preferably the content of betaine in the natural betaine extract is 30wt% - 40wt%.

[0009] Further, the HLB value of the emulsifier is 3.5 - 4.0, preferably the emulsifier is a composite emulsifier, and more preferably the composite emulsifier is any two or three of Span85, Span80 and Tween80.

[0010] According to another aspect of the present application, there is provided a preparation method of a betaine emulsion feed additive as any one of the above, the preparation method comprising: step S1, preparing materials; step S2, adding an emulsifier to the oil and stirring to obtain an emulsified oil; step S3, mixing the emulsified oil with a betaine solution and stirring evenly to obtain a betaine emulsion premix, wherein the content of betaine in the betaine solution is 30 wt% - 50 wt%; step S4, performing a homogenization treatment on the betaine emulsion premix to obtain a betaine emulsion feed additive.

[0011] Further, the emulsifier is a composite emulsifier, and the HLB value of the composite emulsifier is 3.5 - 4.0. Preferably, the emulsifier is any two or three of Span85, Span80, and Tween80.

[0012] Preferably, step S2 includes: step S21, mixing the emulsifiers, the emulsifiers are selected from any two or three of Span85, Span80, and Tween80, and stirring to obtain a composite emulsion, preferably at 50 - 80 °C; step S22, slowly and evenly adding the composite emulsion to the oil and stirring to obtain an emulsified oil.

[0013] Further, in step S2 and / or step S3, the stirring speed is 8000 - 15000 r / min, and the stirring time is 5 - 10 min.

[0014] And / or, step S4 is carried out under the conditions of a temperature of 40 - 85 °C and a pressure of 30 - 50 MPa. Preferably, the oil particle diameter of the betaine emulsion feed additive obtained after homogenization treatment is ≤ 1 μm.

[0015] According to yet another aspect of the present application, there is provided an aquaculture feed, which contains a betaine emulsion feed additive as any one of the above.

[0016] Further, the content of the additive in the feed is 0.1 - 10 wt%, preferably 3 - 10 wt%.

[0017] According to still another aspect of the present application, there is provided a preparation method of the above aquaculture feed, the preparation method comprising spraying a betaine emulsion feed additive as any one of the above on the puffed feed.

[0018] Applying the technical solution of the present invention, a betaine solution with a specific concentration is mixed with oil and an emulsifier to obtain a feed additive in the form of an emulsion. This emulsified feed additive can be added to aquatic extruded feed by spraying during the feed processing, which can replace the process of spraying oil into the extruded feed during the production of aquatic feed. This betaine emulsified feed additive can be added to the feed in a manner similar to that of oil, which is convenient to use, easy to disperse evenly, and is beneficial to the absorption of betaine by the animals being fed. Especially for aquatic animals, it can improve the feeding of aquatic animals, reduce the feed coefficient, accelerate fat metabolism, and reduce liver fat accumulation. Detailed implementation mode

[0019] It should be noted that, without conflict, the embodiments in this application and the features in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the embodiments.

[0020] As analyzed in the background art of this application, in the prior art, betaine is prone to moisture absorption and caking during use, which increases the difficulty in the production process of feed, is difficult to mix evenly with other components, and affects the exertion of its effect. To solve this problem, this application provides a betaine emulsified feed additive, its preparation method and application.

[0021] In a typical implementation mode of this application, a betaine emulsified feed additive is provided, and this additive includes: a betaine solution, soybean oil and an emulsifier, wherein the content of betaine in the betaine solution is 30wt% - 50wt%.

[0022] In this application, a betaine solution with a specific concentration is mixed with oil and an emulsifier to obtain a feed additive in the form of an emulsion. This emulsified feed additive can be added to aquatic extruded feed by spraying during the feed processing, which can replace the process of spraying oil into the extruded feed during the production of aquatic feed. This betaine emulsified feed additive can be added to the feed in a manner similar to that of oil, which is convenient to use, easy to disperse evenly, and is beneficial to the absorption of betaine by the animals being fed. Especially for aquatic animals, it can improve the feeding of aquatic animals, reduce the feed coefficient, accelerate fat metabolism, and reduce liver fat accumulation.

[0023] The ratio of the above-mentioned betaine solution, oil and emulsifier can be determined according to the demand of oil and betaine in the feed in the prior art, so as to determine the dosage of the emulsifier to emulsify the two. In some embodiments of the present application, the weight ratio of the betaine solution to the oil is 1:1 to 10, such as 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9. Within this weight ratio range, the emulsion formed by the betaine solution, oil and emulsifier is more uniform and has a more appropriate concentration, which is convenient for the betaine to play its role, and makes the feed more palatable to the farmed animals, especially aquatic animals; the emulsifier accounts for 1 wt‰ to 10 wt‰ of the total amount of additives, such as 2 wt‰, 3 wt‰, 4 wt‰, 5 wt‰, 6 wt‰, 7 wt‰, 8 wt‰, 9 wt‰, and the emulsification effect is better.

[0024] The above-mentioned betaine solution can be prepared by dissolving solid betaine in water, or directly from the extract of betaine or the solution obtained by separation and purification. Since impurities are easily contained in synthetic betaine, there are potential safety risks, and the cost of separating and purifying high-purity betaine is relatively high. In some preferred embodiments of the present application, the betaine solution is a natural betaine extract. Using a natural betaine extract, the components are derived from plants, and it has a higher safety for use as a feed additive. Moreover, using the extract for the preparation of feed additives eliminates the subsequent purification operation, which is beneficial to reducing costs. Preferably, the content of betaine in the natural betaine extract is 30 wt% to 40 wt%, which is easy to prepare, has a wide source, and is convenient for dispersing with oil to form an emulsion.

[0025] The above-mentioned emulsifier can be selected from the prior art, and can be one kind or a combination of multiple kinds. In some embodiments of the present application, the HLB value of the above-mentioned emulsifier is 3.5 to 4.0. An emulsifier within this HLB value range can easily form a water-in-oil (W / O) emulsion of soybean oil and betaine aqueous solution. Based on the large demand for oil in aquaculture feed and the situation that the water content in the feed is low and difficult to mix with water, making it difficult to meet the water demand of aquatic animals for the feed, when the water-in-oil emulsion of the above-mentioned betaine emulsion feed additive is used in aquaculture feed, it is convenient to mix with other oils required by fish, or evenly disperse in aquaculture feed with a high oil content. That is, the application of the betaine emulsion feed additive emulsified by the above-mentioned emulsifier in aquaculture feed is more convenient. At the same time, it increases the water content in the feed, has a good feeding attraction effect, and is beneficial to the healthy growth of aquatic animals.

[0026] In some embodiments of the present application, the above-mentioned emulsifier is a composite emulsifier. An emulsion prepared by using two or more emulsifiers is more stable, and the proportion of each component in the composite emulsifier is calculated according to the HLB value.

[0027] In some preferred embodiments of the present application, the compound emulsifier is any two or three of Span85, Span80, and Tween80, and the emulsification effect is better. When the compound emulsifier is a combination of Span85 and Tween80, or a combination of Span85 and Span80, the emulsification effect is better, and the emulsified liquid feed additive is more uniform and stable.

[0028] According to a typical implementation manner of the present application, a preparation method of the above-mentioned betaine emulsified liquid feed additive is provided. The preparation method includes: Step S1, preparing materials; Step S2, adding an emulsifier to the oil and stirring to obtain emulsified oil; Step S3, mixing the emulsified oil with a betaine solution and stirring evenly to obtain a betaine emulsified liquid premix, where the content of betaine in the betaine solution is 30 wt% to 50 wt%; Step S4, performing a homogenization treatment on the betaine emulsified liquid premix to obtain the betaine emulsified liquid feed additive.

[0029] By mixing a betaine solution with a specific concentration with oil and an emulsifier through the above preparation method, the layering of the betaine solution and the oil is avoided, and a feed additive in the form of an emulsified liquid is obtained. This emulsified liquid feed additive can be added to aquatic extruded feed by spraying during the feed processing process, and can replace the process of spraying oil into the extruded feed during the production of aquatic feed. The betaine emulsified liquid feed additive can be added to the feed in a manner similar to that of oil, is convenient to use, is easily dispersed evenly, is beneficial for the animals being fed, especially aquatic animals, to absorb, and has a lower cost.

[0030] In some embodiments of the present application, the emulsifier in the above preparation method is a compound emulsifier, and the HLB value of the compound emulsifier is 3.5 to 4.0. By using this emulsifier through the above preparation method, a water-in-oil structure can be formed, and the dispersion degree is better. Preferably, the compound emulsifier is any two or three of Span85, Span80, and Tween80.

[0031] In some preferred embodiments of the present application, Step S2 of the above preparation method includes: Step S21, mixing the emulsifiers, where the emulsifiers are selected from any two or three of Span85, Span80, and Tween80, and stirring to obtain a compound emulsion; Step S22, slowly and evenly adding the compound emulsion to the oil and stirring to obtain emulsified oil. Through the above steps, the compound emulsion can be mixed evenly before being added to the oil, and the emulsification effect is better. In some embodiments of the present application, in order to facilitate the uniform mixing of the compound emulsion, Step S21 is carried out at 50 to 80 °C.

[0032] The above-mentioned step S2 and / or step S3 can be stirred by a stirring device of the prior art, and the specific parameter settings of the stirring can be determined according to the prior art. In some embodiments of the present application, in the above-mentioned step S2 and / or step S3, the stirring speed is 8000-15000 r / min, and the stirring time is 5-10 min. This stirring time is the time for continuous stirring after the materials are mixed.

[0033] The conditions for implementing the above-mentioned step S4 can be determined according to the prior art. In some preferred embodiments of the present application, step S4 is carried out under the conditions of a temperature of 40-85 °C and a pressure (gauge pressure unless otherwise specified) of 30-50 MPa. These temperature and pressure conditions help the oil, emulsifier, and aqueous solution to be evenly dispersed, which is very helpful for the subsequent stability of the product.

[0034] In some embodiments of the present application, in order to make the betaine emulsion feed additive more stable and more convenient for uniform spraying, the oil particle diameter of the betaine emulsion feed additive obtained after homogenization treatment is ≤1 μm.

[0035] According to another typical embodiment of the present application, an aquaculture feed is provided, and this aquaculture feed contains any one of the above-mentioned betaine emulsion feed additives. Applying the above-mentioned betaine emulsion feed additive to the feed for aquaculture can not only play a good role in disease prevention and control, but also improve the palatability of the feed, promote the feeding of aquaculture animals, and improve the feed conversion rate.

[0036] The addition amount of the above-mentioned betaine emulsion feed additive in the feed can be determined according to the prior art. In some embodiments of the present application, the additive is 0.1-10 wt% of the feed, such as 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%. It can achieve a better disease resistance effect and has a better palatability. Preferably, it is 3-10 wt%, and the palatability and disease resistance effects are better.

[0037] According to still another typical embodiment of the present application, a preparation method of the above-mentioned aquaculture feed is provided, and this preparation method includes: spraying any one of the above-mentioned betaine emulsion feed additives on the puffed feed.

[0038] In the processing of aquaculture feed, it is often necessary to spray oil on the puffed feed to improve the nutrition and palatability of the feed. The above-mentioned betaine emulsion feed additive provided by the present application can replace the oil added in the oil spraying process and be added to the feed. While adding the corresponding type of oil to the feed, betaine is evenly added, which can improve the nutritional value of the feed without changing the feed processing process, give full play to the disease resistance effect of betaine, and is especially suitable for aquaculture feed, with a better palatability effect.

[0039] The beneficial effects that can be achieved by the present application will be further described below in combination with examples and comparative examples.

[0040] Example 1

[0041] Prepare the betaine emulsion feed additive according to the following steps:

[0042] (1) Prepare materials: Prepare a betaine solution and soybean oil (low-temperature refined first-grade soybean oil from Shandong Wande Fu Biotechnology Co., Ltd.) in a ratio of 1:1. The betaine solution is a natural betaine extract (from Angel Yeast Co., Ltd.), and the content of betaine in the extract is 30 wt%.

[0043] (2) Preparation of compound emulsifier: Select two emulsifiers, Span85 and Span80 (both purchased from Nantong Huzhuo Chemical Co., Ltd.), and mix them according to the ratio (8:17) calculated based on the HLB value of the compound emulsifier being 3.5, and prepare by uniformly mixing at 50 °C.

[0044] (3) Emulsification: Under the condition that the stirring speed is 8000 r / min, slowly and uniformly add 0.5 part by weight of the compound emulsifier to 50 parts by weight of soybean oil, stir until the emulsifier is fully mixed, obtain emulsified oil, then add the emulsified oil to 50 parts by weight of the betaine solution, and continue to stir for 10 min to mix the soybean oil, emulsifier and betaine solution evenly.

[0045] (4) Homogenization: Perform homogenization treatment at a temperature of 65 °C and a pressure of 50 MPa to obtain the finished product. After homogenization treatment, the oil particle diameter ≤ 1 μm.

[0046] Example 2

[0047] Prepare the betaine emulsion feed additive according to the following steps:

[0048] (1) Prepare materials: Prepare a betaine solution and soybean oil (low-temperature refined first-grade soybean oil from Shandong Wande Fu Biotechnology Co., Ltd.) in a ratio of 1:10. The betaine solution is a natural betaine extract (from Angel Yeast Co., Ltd.), and the content of betaine in the extract is 50 wt%.

[0049] (2) Preparation of compound emulsifier: Select two emulsifiers, Span85 and Tween80 (both purchased from Guangdong Runhua Chemical Co., Ltd.), and mix them according to the ratio (5:1) calculated based on the HLB value of the compound emulsifier being 4.0, and prepare by uniformly mixing at 80 °C.

[0050] (3) Emulsification: Under the condition that the stirring speed is 15,000 r / min, 1.1 parts by weight of the composite emulsifier was slowly and evenly added to 100 parts by weight of soybean oil. After stirring until the emulsifier was fully mixed, emulsified oil was obtained. Then the emulsified oil was added to 10 parts by weight of the betaine solution and stirred continuously for 7 min, and the soybean oil, emulsifier and betaine solution were evenly mixed.

[0051] (4) Homogenization: The finished product was obtained after homogenization treatment at a temperature of 75 °C and a pressure of 40 MPa. After homogenization treatment, the oil particle diameter ≤ 1 μm.

[0052] Example 3

[0053] The difference from Example 1 is that the HLB value of the composite emulsifier is 4, and the ratio of the two emulsifiers Span85 and Tween80 is 5:1.

[0054] Example 4

[0055] The difference from Example 1 is that the HLB value of the composite emulsifier is 3, and the ratio of the two emulsifiers Span85 and Tween80 is 10:1.

[0056] Example 5

[0057] The difference from Example 1 is that the HLB value of the composite emulsifier is 5, and the ratio of the two emulsifiers Span85 and Tween80 is 25:8.

[0058] Example 6

[0059] The difference from Example 1 is that the addition amount of the composite emulsifier is 5 parts by weight.

[0060] Example 7

[0061] The difference from Example 1 is that the betaine solution and soybean oil are equipped in a ratio of 1.5:1.

[0062] Example 8

[0063] The difference from Example 1 is that the temperature during homogenization is 40 °C.

[0064] Example 9

[0065] The difference from Example 1 is that the temperature during homogenization is 30 °C.

[0066] Example 10

[0067] The difference from Example 1 is that the temperature during homogenization is 85 °C.

[0068] The difference between Example 11 and Example 1 is that the stirring speed during emulsification is 6,000 r / min.

[0069] Example 12

[0070] It is different from Example 1 in that the betaine solution is prepared by dissolving synthetic betaine crystals (Henan Haierxi Biotechnology Co., Ltd., 98% betaine) in water.

[0071] It is different from Example 1 in that the emulsifier is Span60 (Guangdong Runhua Chemical Co., Ltd.).

[0072] Comparative Example 1

[0073] It is different from Example 1 in that the content of betaine in the betaine solution is 25 wt%, and the betaine solution is prepared by dissolving naturally purified betaine crystals (Henan Haierxi Biotechnology Co., Ltd., 98% betaine) in water.

[0074] Comparative Example 2

[0075] (1) Preparation of materials: Prepare betaine solution and soybean oil (Shandong Wande Fu Biotechnology Co., Ltd., low-temperature refined first-grade soybean oil) in a ratio of 1:1. The betaine solution is a natural betaine extract (Angel Yeast Co., Ltd.), and the content of betaine in the extract is 40%.

[0076] (2) Emulsification: Under the condition that the stirring speed is 8000 r / min, add 50 parts by weight of soybean oil to 50 parts by weight of betaine solution, and continue to stir for 10 min until the soybean oil and betaine solution are mixed evenly.

[0077] (3) Homogenization: At a temperature of 65 °C and a pressure of 50 MPa, perform homogenization treatment to obtain the finished product. The finished product quickly separates into oil and water layers in a short time after homogenization treatment.

[0078] Comparative Example 3

[0079] Directly use the betaine solution of Example 1 as a feed additive.

[0080] Comparative Example 4

[0081] Directly use the soybean oil of Example 1 as a feed additive.

[0082] Application Example 1

[0083] Add the feed additives prepared in the above examples and comparative examples to the tilapia feed (Jiangmen Xinjian Feed Co., Ltd., tilapia extruded feed No. 0) by post-spraying method, and the addition amount is 3 wt%, and conduct tests.

[0084] The specific experiments are as follows: The experimental fish used were Nile tilapia. Healthy tilapia weighing about 0.3 g were grouped, with 110 - 120 fish in each group. The control group was fed a basal diet (No. 0 extruded tilapia feed from Jiangmen Xinjian Feed Co., Ltd.), and the other example groups or comparative groups were fed the above feed containing additives. Each experimental group was set up with three parallel groups, and each parallel group was placed in a culture tank. At the beginning of the feeding experiment, the fish were fed 3 - 4 times a day, and the feeding amount was 1.5% - 1.8% of the body weight of the fish in each experimental group. The feeding amount was adjusted according to the feeding situation of the fish. The experimental period was 65 days. After the experiment was completed, the weight gain rate, feed conversion ratio, hepatosomatic index, and liver fat content of the experimental fish were measured and calculated. The calculation or measurement methods for the relevant indicators are as follows:

[0085] Weight gain rate (WGR, %) = 100×(Wt - W0) / W0

[0086] Feed conversion ratio (FCR) = F / (Wt + Wd - W0)

[0087] Hepatosomatic index (HSI, %) = (Wz / Wt)×100%

[0088] Where, Wt is the average body weight at the end of the experiment (g); Wd is the average body weight of the dead fish during the experiment (g); W0 is the average initial body weight of the experiment (g); t is the number of experimental days (d); F is the total feed intake (g); Wz is the average weight of the hepatopancreas at the end of the experiment (g).

[0089] Liver fat content: The liver of the fish was taken and determined by referring to the method of GB 5009.6 - 2016 National Food Safety Standard Determination of Fat in Foods.

[0090] The statistical results of the various indicators of each experimental group are shown in Table 1 below.

[0091]

[0092] Application Example 1

[0093] The difference from Application Example 1 is the addition amount of the feed additive. The addition amount of the feed additive in each application group and the test results of the relevant indicators are shown in Table 2. The addition amount of the feed additive in the following table is the addition amount relative to the basal diet (No. 0 extruded tilapia feed from Jiangmen Xinjian Feed Co., Ltd.).

[0094] Table 2

[0095]

[0096]

[0097] From the above description, it can be seen that the above embodiments of the present invention achieve the following technical effects: Mixing a betaine solution with a specific concentration with oil and an emulsifier to obtain a feed additive in the form of an emulsion. This emulsified feed additive can be added to aquatic extruded feed by spraying during the feed processing, which can replace the process of spraying oil into the extruded feed during the production of aquatic feed. The betaine emulsified feed additive can be added to the feed in a manner similar to that of oil, which is convenient to use, easy to disperse evenly, and is beneficial to the absorption of betaine by the animals being fed. Especially for aquatic animals, it can improve the feeding of aquatic animals, reduce the feed coefficient, accelerate fat metabolism, and reduce liver fat accumulation.

[0098] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A betaine emulsion feed additive, characterized in that, The raw materials include: betaine solution, soybean oil and an emulsifier. The content of betaine in the betaine solution is 30wt% - 50wt%; the weight ratio of the betaine solution to the soybean oil is 1:1 - 10, and the emulsifier accounts for 1wt‰ - 10wt‰ of the total amount of the additives; the HLB value of the emulsifier is 3.5 - 4.0, and the emulsifier is a composite emulsifier.

2. The betaine emulsion feed additive according to claim 1, characterized in that, The betaine solution is a natural betaine extract.

3. The betaine emulsion feed additive according to claim 2, characterized in that, The content of betaine in the natural betaine extract is 30wt% - 40wt%.

4. The betaine emulsion feed additive according to claim 1, characterized in that, The composite emulsifier is any two or three of Span85, Span80 and Tween80.

5. A preparation method of the betaine emulsion feed additive according to any one of claims 1 to 4, characterized in that, It includes: Step S1, preparing materials. Step S2, adding the emulsifier to the soybean oil and stirring to obtain emulsified oil, where the emulsifier is a composite emulsifier with an HLB value of 3.5 - 4.

0. Step S3, mixing the emulsified oil with the betaine solution and stirring evenly to obtain a premixed betaine emulsion, where the content of betaine in the betaine solution is 30wt% - 50wt%. Step S4, performing homogenization treatment on the premixed betaine emulsion to obtain the betaine emulsion feed additive.

6. The preparation method according to claim 5, characterized in that, The emulsifier is any two or three of Span85, Span80 and Tween80.

7. The preparation method according to claim 6, characterized in that, The said Step S2 includes: Step S21, mixing the emulsifiers, where the emulsifiers are selected from any two or three of Span85, Span80 and Tween80, and stirring to obtain a composite emulsion. Step S22, slowly and evenly adding the composite emulsion to the soybean oil and stirring to obtain emulsified oil.

8. The preparation method according to claim 7, characterized in that, In the said Step S21, the mixing is carried out at 50 - 80°C.

9. The preparation method according to claim 5, characterized in that, In the said Step S2 and / or Step S3, the stirring speed is 8000 - 15000r / min, and the stirring time is 5 - 10min. And / or, the said Step S4 is carried out under the conditions of a temperature of 40 - 85°C and a pressure of 30 - 50MPa.

10. The preparation method according to claim 9, characterized in that, The oil particle diameter of the betaine emulsion feed additive obtained after the homogenization treatment is ≤1um.

11. An aquaculture feed, characterized in that, It contains the betaine emulsion feed additive as described in any one of claims 1 - 4.

12. The aquaculture feed according to claim 11, wherein The content of the additive in the feed is 0.1 - 10wt%.

13. The aquaculture feed according to claim 12, characterized in that, The content of the additive in the feed is 3 - 10wt%.

14. A method for preparing an aquaculture feed according to any one of claims 11 to 13, characterized in that, Spraying the betaine emulsion feed additive as described in any one of claims 1 - 4 on the puffed feed.

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