Preparation method and application of essential oil composition for repelling water lice

Abstract

The present invention discloses a method for preparing an essential oil composition for repelling water lice. The essential oil composition comprises Acorus calamus essential oil and cinnamon essential oil in a volume ratio of 1:0.01-10. The composition is used by dripping the mixture of Acorus calamus essential oil and cinnamon essential oil onto an easily adsorbed substance. The easily adsorbed substance is packaged in a breathable bag and hung on a mangrove trunk. The essential oil slowly evaporates, releasing an odor that repels water lice, thereby preventing harm to the mangroves. The composition is environmentally friendly, has no toxic side effects, is simple to use, is low-cost, and has a good repellent effect on water lice.

Description

Technical Field

[0001] The present invention relates to the field of pesticides, and in particular to a preparation method and application of an essential oil composition for repelling water lice. Background Art

[0002] Sphaeromatidae are one of the most common groups of crustaceans in the order Isopoda, belonging to the phylum Arthropoda, class Crustatacea, order Isopoda, and family Sphaeromatidae. A total of 93 genera and 634 species of Sphaeromatidae have been reported worldwide, including 12 species in my country belonging to eight genera, five of which are new to China. The main species of Sphaeromatidae that infect Hainan and Guangxi are two species of the genus Sphaeroma: Sphaeroma terebrans and Sphraeroma retrolaevis. Sphaeromatidae can damage a variety of mangroves, boring into the base of their roots and aerial roots. These damaged mangroves are severely damaged, and after external factors such as typhoons, trees fall and die in large areas, forming forest gaps. The water louse not only has a devastating destructive effect on mangroves, but also accelerates coastal erosion. It is an important landmark in marine pollution control.

[0003] Numerous scholars have proposed control measures for mangrove water lice. Lü Xiaobo et al., "A physical control method for mangrove water lice using the isolation principle, CN104067889A, 2014," uses plastic film to physically isolate water lice. Liu Wen'ai et al., "A method for controlling mangrove water lice, CN105379591A, 2016," uses salt for smearing or spraying. Guan Wei et al., "A smoke agent, fumigation device, and method for rapid control of water lice, CN105961432A, 2016," uses fumigation just after low tide for two hours to quickly and effectively kill mangrove water lice. He Xuexiang et al., "Dual agent for controlling water lice and method for controlling water lice using the same, CN106417367A, 2017," prepares a chemical agent for application. Huang Bo et al., "Medications for controlling water lice and shipworms, and methods of their use, CN104397033A, 2018," prepare a drug for application and then apply tung oil for isolation. Liu Wen'ai et al., "A method for controlling water lice in mangroves using chlorine dioxide, CN107996223A, 2018," prepare a high-concentration chlorine dioxide solution for spraying. These control methods primarily focus on killing water lice organisms, requiring individual mangroves to be treated, which is inconvenient and costly. Furthermore, some control agents and auxiliary substances, such as arsenic and formaldehyde, are significantly toxic. Such control techniques cannot fundamentally address the hazards of water lice and also have some adverse effects on the local mangrove ecosystem. Therefore, continued research and exploration of more efficient and environmentally friendly technologies is necessary.

[0004] Based on the above, the present invention will make full use of the repellent properties of essential oils in nature and apply them to areas where mangroves are seriously damaged, reduce the population density of water lice, alleviate the damage caused by water lice, enhance the growth of mangroves, and achieve the purpose of protecting mangroves. Summary of the Invention

[0005] In view of this, the present invention proposes a preparation method and application of essential oil for repelling water lice to solve the above problems.

[0006] The technical solution of the present invention is achieved as follows:

[0007] The invention discloses an essential oil composition for repelling water lice, comprising Acorus calamus essential oil and cinnamon essential oil.

[0008] Furthermore, the volume ratio of the Acorus calamus essential oil to the cinnamon essential oil is 1:0.01-10.

[0009] Furthermore, the preparation method of the Acorus calamus essential oil comprises the following steps:

[0010] (1) drying and crushing the rhizome of Acorus calamus to obtain rhizome powder, and drying and crushing the leaves of Acorus calamus to obtain leaf powder;

[0011] (2) mixing the Acorus calamus rhizome powder and water in a mass ratio of 1:15-20 to obtain a Acorus calamus rhizome powder mixture, soaking for 2-3 hours, steaming at a temperature of 80-90° C., and distilling for 2-3 hours to obtain Acorus calamus rhizome essential oil;

[0012] (3) mixing Acorus calamus leaf powder and water in a mass ratio of 1:5-8 to obtain an Acorus calamus leaf powder mixture, steaming at a temperature of 50-60° C. for 70-90 min to obtain Acorus calamus leaf essential oil;

[0013] (4) Mixing the Acorus calamus leaf essential oil and the Acorus calamus rhizome essential oil to prepare a finished product.

[0014] Furthermore, the preparation method of the cinnamon essential oil comprises the following steps:

[0015] (1) crushing cinnamon leaves to obtain cinnamon leaf powder; crushing cinnamon bark to obtain cinnamon bark powder;

[0016] (2) mixing cinnamon leaf powder and 40-50 wt% ethanol solution, heating to 45-55° C. at 2-4° C. / min, maintaining for 30-40 min, and further heating to 80-85° C. at 0.8-1.2° C. / min, maintaining for 90-120 min, to obtain cinnamon leaf essential oil;

[0017] (3) extracting the cinnamon bark powder by supercritical extraction at an extraction temperature of 45-50° C., an extraction pressure of 22-25 MPa, a carbon dioxide flow rate of 8-10 L / h, and an extraction time of 2-3 h, and separating to obtain cinnamon bark essential oil;

[0018] (4) Mixing cinnamon leaf essential oil and cinnamon bark essential oil to obtain a finished product.

[0019] Furthermore, in step (2), the mass ratio of the cinnamon leaf powder to the 40-50 wt% ethanol solution is 1:7-9.

[0020] Furthermore, in step (3), the entrainer is a 4-5 wt% ethanol solution, and the mass ratio of the entrainer to the cinnamon bark powder is 0.008-0.012:1.

[0021] Furthermore, in step (3), the separation pressure is 8-12 MPa, and the separation temperature is 40-50°C.

[0022] Furthermore, calamus essential oil and cinnamon essential oil are dripped onto easily adsorbable substances, which are then packaged in breathable bags and hung on the trunks of mangrove plants. The essential oils evaporate slowly, releasing odors to repel water lice.

[0023] Furthermore, the easily adsorbable substances include absorbent cotton, qualitative filter paper, activated carbon, hydrophobic fumed silica powder, diatomaceous earth, and cellulose.

[0024] Beneficial effects of the present invention:

[0025] (1) The present invention combines Acorus calamus essential oil and cinnamon essential oil, which have repellent activity against water lice, to repel water lice that harm mangrove plants and avoid the use of chemical pesticides and other toxic substances.

[0026] (2) The materials used in the present invention are all derived from plant materials, are environmentally friendly, belong to the category of green pesticides, and have abundant material sources.

[0027] (3) The method of using the present invention is simple and easy. By hanging the bag, the essential oil adsorbed on materials such as cotton wool is slowly released, thereby achieving the purpose of long-term control of the water lice population.

[0028] (4) In the present invention, the Acorus calamus essential oil and the cinnamon essential oil are extracted by different methods according to the characteristics of different parts of the raw materials, thereby improving the oil yield and effective ingredient content of the finished product, and further improving the repellent effect of the Acorus calamus essential oil and the cinnamon essential oil on water lice. DETAILED DESCRIPTION

[0029] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0030] Example 1 Preparation method of Acorus calamus essential oil

[0031] Experimental Group 1:

[0032] (1) drying and crushing the rhizome of Acorus calamus to obtain rhizome powder, and drying and crushing the leaves of Acorus calamus to obtain leaf powder;

[0033] (2) mixing the Acorus calamus rhizome powder and water in a mass ratio of 1:15 to obtain a Acorus calamus rhizome powder mixture, soaking for 2 h, steaming at 80° C., and distilling for 2 h to obtain Acorus calamus rhizome essential oil;

[0034] (3) Acorus calamus leaf powder and water were mixed in a mass ratio of 1:5 to obtain a Acorus calamus leaf powder mixture, the steam temperature was 50°C, and the distillation time was 70 min to obtain Acorus calamus leaf essential oil;

[0035] (4) The Acorus calamus leaf essential oil and the Acorus calamus rhizome essential oil are mixed in a mass ratio of 1:1 to obtain a finished product.

[0036] Experimental Group 2

[0037] (1) drying and crushing the rhizome of Acorus calamus to obtain rhizome powder, and drying and crushing the leaves of Acorus calamus to obtain leaf powder;

[0038] (2) mixing the Acorus calamus rhizome powder and water in a mass ratio of 1:20 to obtain a Acorus calamus rhizome powder mixture, soaking for 3 h, steaming at 90° C., and distilling for 3 h to obtain Acorus calamus rhizome essential oil;

[0039] (3) Acorus calamus leaf powder and water were mixed in a mass ratio of 1:8 to obtain a Acorus calamus leaf powder mixture, the steam temperature was 60°C, and the distillation time was 90 min to obtain Acorus calamus leaf essential oil;

[0040] (4) The Acorus calamus leaf essential oil and the Acorus calamus rhizome essential oil are mixed in a mass ratio of 1:1 to obtain a finished product.

[0041] Experimental Group 3

[0042] (1) drying and crushing the rhizome of Acorus calamus to obtain rhizome powder, and drying and crushing the leaves of Acorus calamus to obtain leaf powder;

[0043] (2) mixing Acorus calamus rhizome powder and water in a mass ratio of 1:18 to obtain a mixed solution of Acorus calamus rhizome powder, soaking for 2.5 h, steaming at 85 ° C, and distilling for 2.5 h to obtain Acorus calamus rhizome essential oil;

[0044] (3) Acorus calamus leaf powder and water were mixed in a mass ratio of 1:6 to obtain a Acorus calamus leaf powder mixture, and the steam temperature was 55°C and the distillation time was 80 min to obtain Acorus calamus leaf essential oil;

[0045] (4) Mixing the Acorus calamus leaf essential oil and the Acorus calamus rhizome essential oil in a mass ratio of 1:1 to obtain a finished product.

[0046] Experimental Group 4

[0047] (1) drying and crushing the rhizomes and leaves of Acorus calamus to obtain Acorus calamus powder;

[0048] (2) The rhizomes, stems and leaves of Acorus calamus and water were mixed in a mass ratio of 1:18 to prepare a mixture of Acorus calamus powder, which was soaked for 2.5 h, steamed at 85 °C, and distilled for 2.5 h to obtain Acorus calamus essential oil.

[0049] Essential oil extraction rate (%) = finished product weight / raw material weight × 100

[0050] name Experimental Group 1 Experimental Group 2 Experimental Group 3 Experimental Group 4 Essential oil extraction rate (%) 3.21 3.14 3.31 2.89

[0051] The experimental results show that the present invention can improve the extraction rate of Acorus calamus essential oil by adopting different methods to extract Acorus calamus essential oil according to the different properties of Acorus calamus rhizomes and leaves. After experimental analysis, the Acorus calamus essential oil prepared by the method of the present invention has a good repellent effect on water lice.

[0052] Example 2 Preparation method of cinnamon essential oil

[0053] Experimental Group 1

[0054] (1) crushing cinnamon leaves to obtain cinnamon leaf powder; crushing cinnamon bark to obtain cinnamon bark powder;

[0055] (2) Cinnamon leaf powder and 40 wt% ethanol solution were mixed in a mass ratio of 1:7, heated to 45°C at 2°C / min, maintained for 30 min, and then heated to 80°C at 0.8°C / min, maintained for 90 min, to obtain cinnamon leaf essential oil;

[0056] (3) The cinnamon bark powder was extracted by supercritical extraction, 4 wt% ethanol solution was added to the reactor, the mass ratio of 4 wt% ethanol solution to cinnamon bark powder was 0.008:1, the extraction temperature was 45 ° C, the extraction pressure was 22 MPa, the carbon dioxide flow rate was 8 L / h, the extraction time was 2 h, and the separation pressure was 8 MPa and the separation temperature was 40 ° C to obtain cinnamon bark essential oil;

[0057] (4) Cinnamon leaf essential oil and cinnamon bark essential oil are mixed in a mass ratio of 1:1 to obtain a finished product.

[0058] Experimental Group 2

[0059] (1) crushing cinnamon leaves to obtain cinnamon leaf powder; crushing cinnamon bark to obtain cinnamon bark powder;

[0060] (2) Cinnamon leaf powder and 50 wt% ethanol solution were mixed in a mass ratio of 1:9, heated to 55°C at a heating rate of 4°C / min, maintained for 40 min, and then heated to 85°C at a heating rate of 1.2°C / min, maintained for 120 min, to obtain cinnamon leaf essential oil;

[0061] (3) The cinnamon bark powder was extracted by supercritical extraction, 5 wt% ethanol solution was added to the reactor, the mass ratio of 5 wt% ethanol solution to cinnamon bark powder was 0.012:1, the extraction temperature was 50 ° C, the extraction pressure was 25 MPa, the carbon dioxide flow rate was 10 L / h, the extraction time was 3 h, and the separation pressure was 12 MPa and the separation temperature was 50 ° C to obtain cinnamon bark essential oil;

[0062] (4) Cinnamon leaf essential oil and cinnamon bark essential oil are mixed in a mass ratio of 1:1 to obtain a finished product.

[0063] Experimental Group 3

[0064] (1) crushing cinnamon leaves to obtain cinnamon leaf powder; crushing cinnamon bark to obtain cinnamon bark powder;

[0065] (2) Cinnamon leaf powder and 45 wt% ethanol solution were mixed in a mass ratio of 1:8, heated to 50°C at 3°C / min, maintained for 35 min, and then heated to 85°C at 1°C / min, maintained for 110 min, to obtain cinnamon leaf essential oil;

[0066] (3) The cinnamon bark powder was extracted by supercritical extraction, 4.5 wt% ethanol solution was added to the reactor, the mass ratio of 4.5 wt% ethanol solution to cinnamon bark powder was 0.01:1, the extraction temperature was 48 ° C, the extraction pressure was 24 MPa, the carbon dioxide flow rate was 9 L / h, the extraction time was 2.5 h, and the separation pressure was 10 MPa and the separation temperature was 45 ° C to obtain cinnamon bark essential oil;

[0067] (4) Cinnamon leaf essential oil and cinnamon bark essential oil are mixed in a mass ratio of 1:1 to obtain a finished product.

[0068] Experimental Group 4

[0069] (1) crushing cinnamon leaves and cinnamon bark to prepare cinnamon powder;

[0070] (2) Cinnamon powder is extracted by supercritical extraction, 4.5wt% ethanol solution is added to the reactor, the mass ratio of 4.5wt% ethanol solution to cinnamon bark powder is 0.01:1, the extraction temperature is 48°C, the extraction pressure is 24MPa, the carbon dioxide flow rate is 9L / h, the extraction time is 2.5h, and the separation pressure is 10MPa and the separation temperature is 45°C to obtain cinnamon essential oil.

[0071] Essential oil extraction rate (%) = finished product weight / raw material weight × 100

[0072] name Experimental Group 1 Experimental Group 2 Experimental Group 3 Experimental Group 4 Essential oil extraction rate (%) 7.95 8.17 8.33 7.31

[0073] Experimental results show that the present invention can improve the extraction rate of cinnamon oil by extracting cinnamon essential oil using different methods according to the different properties of cinnamon bark and leaves. After experimental analysis, the cinnamon essential oil prepared by the method of the present invention has a good repellent effect on water lice.

[0074] Example 3

[0075] Add some Acorus calamus essential oil to some absorbent cotton, place it in a waterproof and breathable bag, and hang it on the trunk of the mangrove for use.

[0076] Example 4

[0077] Acorus calamus essential oil and cinnamon essential oil are mixed in a volume ratio of 1:1 to prepare an essential oil composition, which is then dripped onto absorbent cotton, placed in a waterproof and breathable bag, and hung on a mangrove trunk for use.

[0078] Example 5

[0079] Acorus calamus essential oil and cinnamon essential oil are mixed in a volume ratio of 1:5 to prepare an essential oil composition, which is then dripped onto absorbent cotton, placed in a waterproof and breathable bag, and hung on the trunk of a mangrove for use.

[0080] Example 6

[0081] The essential oil composition is prepared by mixing Acorus calamus essential oil and cinnamon essential oil in a volume ratio of 1:10, then dripping the mixture onto absorbent cotton, placing the mixture in a waterproof breathable bag, and hanging the mixture on the trunk of a mangrove for use.

[0082] The Acorus calamus essential oil used in Examples 4-7 was the Acorus calamus essential oil prepared in Experimental Group 3 in Example 1, and the cinnamon essential oil used in Examples 5-7 was the cinnamon essential oil prepared in Experimental Group 3 in Example 2.

[0083] Test Example 1: Behavioral response of indoor water lice to essential oil compositions

[0084] A Y-shaped olfactometer was used to test the behavioral responses of water lice to the essential oil composition. The arms of the tubes consisted of silicone tubing with an inner diameter of 2 cm. One arm served as the treatment group, and the other served as the control group. A single water lice of uniform size was selected and placed in the straight arm. 10 μL of the essential oil composition was dripped onto a cotton ball as the treatment group, while 10 μL of distilled water was used as the control group. The air flow rate was maintained at 0.1 L / min. Recordings were made every 10 minutes, with each treatment repeated 10 times. The behavioral responses of the water lice were observed and the response rate was calculated, with the selected response rate and avoidance response rate being used.

[0085]

[0086]

[0087]

[0088] During the experiment, water lice that remained in the straight arm were considered non-reactive, those that entered the treatment group were considered selectively reactive, and those that entered the control group were considered avoidant. After each test insect was cleaned with alcohol, the two arms were swapped, alternating use to minimize external influences.

[0089] As shown in Table 1, there are significant differences in the responses of different examples to water lice. 2The test and P value showed that there were extremely significant differences in the selection response and repellent response of the essential oil compositions of Examples 3-6 to Daphnia gracilis, and both showed significant repellent effects.

[0090] Table 1: Behavioral responses of the essential oil compositions of Examples 3-6 to water lice in the room

[0091]

[0092] Test Example 2: Behavioral responses of field water lice to essential oils

[0093] An easily absorbable material containing the water lice repellent essential oil from Examples 3-6 was placed in a waterproof, breathable bag. Each bag contained 10 mL of water lice repellent essential oil and was hung on the trunk of a dead mangrove tree infested with water lice. The number of water lice in the holes was checked every seven days, and the water lice reduction rate per square decimeter was calculated. Reduction rate (%) = (number of water lice in the control group - number of water lice in the treated group) / number of water lice in the control group × 100%.

[0094] As shown in Table 2, the essential oil composition has a significant repellent effect on water lice. After 7 days of treatment, the water lice reduction rate reached 24.07% (Example 6), with Example 4 achieving the highest reduction rate of 42.59%. After 14 days, there was no significant difference between Examples 4 and 5, with a water lice reduction rate of approximately 55%. After 21 days, Example 4 showed the highest effect, with a water lice reduction rate of 56.86%. Over time, the water lice reduction rate increased, indicating a stronger repellent effect of the essential oil composition. Comparison of the treatments after 14 and 21 days revealed a reduced range of change in the reduction rate, indicating that the essential oil composition gradually stabilized and was slowly released within the breathable bag.

[0095] Table 2: Repellent effect of the essential oil compositions of Examples 3-6 on water lice in the field

[0096]

[0097] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An application of an essential oil composition in repelling water lice, characterized in that: The essential oil composition comprises Acorus calamus essential oil and cinnamon essential oil in a volume ratio of 1:0.01-10; The preparation method of the Acorus calamus essential oil comprises the following steps: (1) drying and crushing the rhizome of Acorus calamus to obtain rhizome powder, and drying and crushing the leaves of Acorus calamus to obtain leaf powder; (2) mixing Acorus calamus rhizome powder and water in a mass ratio of 1:15-20 to obtain Acorus calamus rhizome powder mixture, soaking for 2-3 hours, steaming at a temperature of 80-90°C, and distilling for 2-3 hours to obtain Acorus calamus rhizome essential oil; (3) Mixing Acorus calamus leaf powder and water in a mass ratio of 1:5-8 to obtain Acorus calamus leaf powder mixture, steaming at a temperature of 50-60°C and distilling for 70-90 min to obtain Acorus calamus leaf essential oil; (4) mixing the Acorus calamus leaf essential oil and the Acorus calamus rhizome essential oil to prepare a finished product; The preparation method of the cinnamon essential oil comprises the following steps: (1) Crush cinnamon leaves to obtain cinnamon leaf powder; crush cinnamon bark to obtain cinnamon bark powder; (2) Mixing cinnamon leaf powder and 40-50 wt% ethanol solution, heating to 45-55°C at 2-4°C / min, maintaining for 30-40 min, and further heating to 80-85°C at 0.8-1.2°C / min, maintaining for 90-120 min, to obtain cinnamon leaf essential oil; (3) extracting the cinnamon bark powder by supercritical extraction at an extraction temperature of 45-50°C, an extraction pressure of 22-25 MPa, a carbon dioxide flow rate of 8-10 L / h, and an extraction time of 2-3 h, and separating to obtain cinnamon bark essential oil; (4) Mixing cinnamon leaf essential oil and cinnamon bark essential oil to obtain a finished product.

2. The use according to claim 1, characterized in that In step (2) of the preparation method of cinnamon essential oil, the mass ratio of the cinnamon leaf powder to the 40-50wt% ethanol solution is 1:7-9.

3. The use according to claim 1, characterized in that In step (3) of the preparation method of cinnamon essential oil, the entrainer is a 4-5wt% ethanol solution, and the mass ratio of the entrainer to the cinnamon bark powder is 0.008-0.012:

1.

4. The use according to claim 1, wherein In step (3) of the method for preparing cinnamon essential oil, the separation pressure is 8-12 MPa and the separation temperature is 40-50°C.

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