A preparation method of rose polysaccharide and its application in whitening and anti-oxidation

Abstract

The present invention belongs to the technical field of natural product extraction and cosmetic preparation, and specifically relates to a method for preparing rose polysaccharide and its application in whitening and anti-oxidation. The present invention uses rose waste liquid as a raw material, and extracts and prepares rose polysaccharide therefrom by optimizing process conditions. Experimental verification shows that the rose polysaccharide extracted from rose waste liquid has whitening and anti-oxidation effects, and in particular, the rose polysaccharide component 2 of the rose waste liquid obtained by purification has the best whitening and anti-oxidation effects, so it can be used to prepare cosmetics with whitening and anti-oxidation effects. Rose polysaccharide has good practical application prospects, and the preparation of rose polysaccharide effectively improves the comprehensive utilization rate of roses.

Description

Technical Field

[0001] The invention belongs to the technical field of natural product extraction and cosmetic preparation, and particularly relates to a preparation method of rose polysaccharide and application thereof in whitening and anti-oxidation. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Roses are native to China and have a long history of cultivation in my country. They are now grown all over the country. Roses are not only excellent plant materials for landscaping and soil and water conservation, but are also widely recognized by consumers as precious Chinese medicinal materials, food, and spices.

[0004] Roses have been recognized for their beauty and skin care effects since ancient times. In the rose planting industry, applying roses to the cosmetics industry is one of the main ways for roses to generate economic benefits. However, the specific components of roses with beauty effects have not yet been fully elucidated. Rose essential oil is a rose beauty product currently being industrialized, and it is widely used as a beauty product or cosmetic additive. Rose essential oil is obtained by distillation and extraction of roses, and is rich in active chemical components of roses such as terpenes, alcohols, and ethers. However, the yield of rose essential oil is very low, generally only about 0.015-0.04%, and it takes several tons of roses to extract one liter of essential oil. After the essential oil is extracted from roses, a large amount of rose water-soluble components are discarded. Roses are extremely precious natural resources. Do the water-soluble components of roses contain skin care ingredients? Can the water-soluble components of roses with beauty effects be extracted to further improve the utilization rate of roses? At present, there are few studies on the application of water-soluble components of roses in the field of cosmetics.

[0005] Rose polysaccharide is one of the main water-soluble components of roses. With the improvement of people's living standards, more and more attention has been paid to finding safe and effective cosmetic additives from natural ingredients. Polysaccharides have good biocompatibility, biodegradability and are mild and non-irritating. Natural polysaccharides such as hyaluronic acid and chitosan have been widely used in the cosmetics industry as moisturizers, thickeners and stabilizers. Polysaccharides have good application prospects in the cosmetics industry.

[0006] However, the inventor found that there are few reports on the efficacy of rose polysaccharides in the cosmetics industry. The inventor previously applied for and authorized a CN 113717297 B "Rose polysaccharide and its preparation method and application", which disclosed that rose polysaccharides have anti-inflammatory and in vitro antioxidant effects. However, developing more efficacy of rose polysaccharides, especially expanding its application in the cosmetics field, will help further improve the utilization rate of roses, increase the added value of roses, and extend the rose industry chain. Summary of the invention

[0007] In view of the deficiencies of the prior art, the present invention provides a method for preparing rose polysaccharide and its application in whitening and anti-oxidation. The present invention finds through experimental research that rose polysaccharide and its components extracted from rose waste liquid have whitening and anti-oxidation effects, and can be used to prepare cosmetics with whitening and anti-oxidation effects. Based on the above research results, the present invention is completed.

[0008] In order to achieve the above technical objectives, the technical solution adopted by the present invention is as follows:

[0009] The first aspect of the present invention provides the use of rose polysaccharide in preparing cosmetics with whitening and / or anti-oxidation effects.

[0010] The rose polysaccharide is a polysaccharide component extracted from roses, wherein the roses should be understood in a broad sense, i.e., it includes any roses and products thereof that may contain rose polysaccharides (including byproducts and wastes generated in the production process of rose products). Therefore, the roses can be rose waste liquid generated in the industrial production of roses (such as the extraction and production of rose essential oil).

[0011] The second aspect of the present invention provides a method for preparing rose polysaccharide, which at least comprises separating and obtaining rose polysaccharide from rose waste liquid.

[0012] The third aspect of the present invention provides a cosmetic having whitening and / or anti-oxidation effects, wherein the cosmetic at least comprises the above-mentioned rose polysaccharide.

[0013] In the present invention, the cosmetics should be understood in a broad sense, including but not limited to cleansing cream (cream), facial cleanser, eye cream, shampoo (cream), conditioner, hand soap, hand cream, body lotion, bath gel, facial cream, lotion and facial mask, etc.

[0014] Beneficial technical effects of one or more of the above technical solutions:

[0015] The above technical solution uses rose waste liquid as raw material to extract and prepare rose polysaccharide. Experimental verification shows that the rose polysaccharide extracted from rose waste liquid has whitening and antioxidant effects, and can be used to prepare cosmetics with whitening and antioxidant effects. Therefore, rose waste liquid rose polysaccharide has good practical application prospects and effectively improves the comprehensive rate of roses. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0017] Figure 1 This is the elution curve of the DEAE-52 cellulose anion exchange column in Example 1 of the present invention.

[0018] Figure 2 This is the HPSEC-MALLS chromatogram of rose polysaccharide and its components 1 and 2 in Example 2 of the present invention, wherein a is rose polysaccharide from rose waste liquid; b is rose polysaccharide component 1 from rose waste liquid; and c is rose polysaccharide component 2 from rose waste liquid.

[0019] Figure 3 The hydroxyl radical scavenging ability of rose polysaccharide and its components 1 and 2 in Example 3 of the present invention.

[0020] Figure 4 It is the total antioxidant capacity of rose polysaccharide and its components 1 and 2 in Example 4 of the present invention.

[0021] Figure 5 This is the melanin inhibition ability of rose polysaccharide component 2 from rose waste liquid in Example 5 of the present invention in zebrafish.

[0022] Figure 6 This is the melanin inhibition ability of rose polysaccharide from rose waste liquid in zebrafish in Example 6 of the present invention.

[0023] Figure 7 This is the in vivo antioxidant capacity of rose polysaccharide component 2 from rose waste liquid in Example 7 of the present invention.

[0024] Figure 8 This is the in vivo antioxidant capacity of rose polysaccharides from rose waste liquid in Example 8 of the present invention. DETAILED DESCRIPTION

[0025] It should be noted that the following detailed descriptions are all illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0026] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof. It should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the embodiments of the present invention are intended to describe specific embodiments, rather than to limit the scope of protection of the present invention.

[0027] In a typical embodiment of the present invention, there is provided the use of rose polysaccharide in the preparation of cosmetics with whitening and / or antioxidant effects.

[0028] In another specific embodiment of the present invention, a method for preparing rose polysaccharide is provided, and the preparation method at least comprises separating and obtaining rose polysaccharide from rose waste liquid.

[0029] Specifically, when rose polysaccharides are separated from rose waste liquid, the preparation method specifically includes:

[0030] S1. Add ethanol to the rose waste liquid, mix and let stand, collect the precipitate by centrifugation and dry to obtain a solid;

[0031] S2, after the solid is redissolved, the protein is removed and the solid is dried to obtain the product.

[0032] Wherein, in the step S1,

[0033] The rose waste liquid is specifically the rose waste liquid generated during the extraction of rose essential oil. Further, the rose is placed in a rose essential oil / rose water distillation device, 1-5 times (preferably 2.5 times, mass volume ratio, g / mL) of water is added, and the rose cooking residue is obtained by heating at 90-100° C. (preferably 100° C.) for 1-5 h (preferably 3 h). The rose cooking residue is filtered, and the filtrate is the rose waste liquid.

[0034] The ethanol is a high concentration ethanol. The ethanol can be 70% or above ethanol, such as 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% ethanol. In a specific embodiment of the present invention, the ethanol is 95% ethanol.

[0035] The volume ratio of the rose waste liquid to ethanol is 1:1-5, such as 1:1, 1:2, 1:3, 1:4, 1:5. For example, in a specific embodiment of the present invention, the volume ratio of the rose waste liquid to ethanol is 1:3.

[0036] The standing time is 1-36h, such as 3h, 8h, 10h, 12h, 16h, 18h, 24h, 36h. In a specific embodiment of the present invention, the standing time is 12h.

[0037] The specific centrifugal conditions are 8000-12000 r / min for 5-20 min, preferably 10000 r / min for 10 min.

[0038] The drying can be carried out by drying or freeze-drying. In a specific embodiment of the present invention, the drying process is selected.

[0039] In step S2, the specific method for re-dissolving the solid includes adding the solid into deionized water, and the mass volume ratio of the solid to deionized water is 1:10-100 (preferably 1:50, g / mL).

[0040] The deproteinization method may be any method known in the art, such as the Sevag method. The drying may be performed by drying or freeze drying. In a specific embodiment of the present invention, freeze drying is used.

[0041] In order to further purify the obtained rose polysaccharide, column chromatography was used for separation and purification. Specifically, the rose polysaccharide was separated and purified by DEAE-52 cellulose ion exchange column chromatography, dialyzed and freeze-dried. At this time, the rose polysaccharide was purified into two main components, which were named rose waste liquid rose polysaccharide component 1 and rose waste liquid rose polysaccharide component 2.

[0042] Specifically, the specific purification process of the above two components is as follows:

[0043] Water and 0.1, 0.3, and 0.5 mol / L NaCl solutions are used in sequence for elution, the flow rate of the eluent is 0.5-2 mL / min (preferably 1 mL / min), 25-35 tubes (preferably 30 tubes) of eluent are collected for each elution concentration, the volume of each tube is 0.5-4 mL (preferably 2 mL), 5-25 tubes are collected, dialyzed and freeze-dried, which is the rose waste liquid rose polysaccharide component 1; 35-55 tubes are collected, dialyzed and freeze-dried, which is the rose waste liquid rose polysaccharide component 2.

[0044] The rose polysaccharide from the rose waste liquid prepared by the above method showed good ability to inhibit melanin formation in the zebrafish melanin formation inhibition test, and thus can be used as a raw material for whitening and lightening cosmetics; at the same time, in the zebrafish skin oxidative damage test mediated by metronidazole, it was also proved that the rose polysaccharide prepared by the above method had good in vivo antioxidant effect, especially the whitening and antioxidant effects of rose polysaccharide component 2 from the rose waste liquid were the most prominent. In short, the above experiments fully prove that the rose polysaccharide from the rose waste liquid of the present invention has broad market value in the development of whitening and lightening and / or antioxidant cosmetics, and therefore has good practical application value.

[0045] In another specific embodiment of the present invention, a cosmetic having whitening and / or anti-oxidation effects is provided, wherein the cosmetic at least comprises the above-mentioned rose polysaccharide.

[0046] In the present invention, the cosmetics should be understood in a broad sense, including but not limited to cleansing cream (cream), facial cleanser, eye cream, shampoo (cream), conditioner, hand soap, hand cream, body lotion, bath gel, facial cream, lotion and facial mask, etc.

[0047] In addition to the rose polysaccharide component, the present invention may optionally contain common ingredients of cosmetics, including any ingredients known in the art such as vehicles, surfactants and cosmetic excipients, and their types and amounts can be selected according to specific needs. Generally, the content of the common ingredients is 1-95% by weight based on the total weight of the composition.

[0048] For example, the vehicle includes but is not limited to a diluent, a dispersant or a carrier, and the like, including but not limited to ethanol, butanediol, dipropylene glycol, pentanediol, and the like.

[0049] The cosmetic auxiliary materials include but are not limited to emulsifiers, thickeners and the like.

[0050] The emulsifier includes, but is not limited to, one or more combinations of glyceryl stearate / PEG-100 stearate, PPG-13-decyltetradecyl alcohol polyether-24, cetearyl glucoside, polyglyceryl-10 myristate, cetearyl glucoside, polyglyceryl-10 stearate, polyglyceryl-10 dioleate, etc. Those skilled in the art can specifically select the type and amount thereof as needed.

[0051] The thickener includes, but is not limited to, one or more combinations of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, hydroxyethyl cellulose, hydroxypropyl cellulose, carbomer, gum arabic, polyethylene glycol-14M, polyethylene glycol-90M, succinoglycan, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, etc. Those skilled in the art can specifically select the type and amount thereof according to the needs.

[0052] The present invention is further explained by the following examples, but they are not intended to limit the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0053] Embodiment 1:

[0054] Rose waste liquid (rose waste liquid is the rose waste liquid generated during the extraction of rose essential oil - place roses in a rose essential oil / rose water distillation device, add 2.5 times (M / V, g / mL) deionized water, heat at 100°C for 3h to obtain rose cooking residue, filter the rose cooking residue, and the filtrate is rose cooking water, i.e., rose waste liquid), add 95% ethanol in proportion (rose waste liquid: ethanol is 1:3, V / V). Stand overnight, centrifuge (10000r / min, 10min), dry the precipitate (55°C), redissolve in deionized water (the mass volume ratio of the dried precipitate to the deionized water is 1:50, g / mL), remove protein by Sevage method, and freeze-dry to obtain rose waste liquid rose polysaccharide.

[0055] The rose waste liquid rose polysaccharide solution was filtered (0.45 μm), and the filtrate was added to a DEAE-52 cellulose anion exchange column (1.6 cm×30 cm), and eluted with deionized water and different concentrations of NaCl solution (0.1, 0.3 and 0.5 mol / L), the elution rate was 1 mL / min, 2 mL was collected in each tube, and 30 tubes were collected in one concentration gradient. The absorbance was determined by the phenol-sulfuric acid method, and the elution curve of the DEAE-52 cellulose anion exchange column was drawn with the number of test tubes as the horizontal coordinate and the concentration and absorbance of the eluent as the vertical coordinate. Component 1 (tube 5-tube 25) and component 2 (tube 35-tube 55) were collected, and the rose waste liquid rose polysaccharide component 1 and component 2 were obtained by lyophilization after dialysis.

[0056] Embodiment 2:

[0057] The molecular weight of rose waste liquid rose polysaccharide and rose waste liquid rose polysaccharide component 1 and component 2 were determined by high performance liquid chromatography coupled with multi-angle laser light scattering (HPSEC-MALLS), using an 18-angle laser light scattering high gel permeation chromatography system equipped with a GPC gel permeation chromatograph unit infusion pump (Waters515), an 18-angle laser light scattering instrument (WyattDawn Heleos11), a differential refractometer (Optilab T-rex) and a Shodex SBOHPAK-806-803 column. Mobile phase: ultrapure water (0.02% sodium azide), pH=6; column temperature: 40°C; flow rate: 1.0 mL / min; injection volume: 500 μL.

[0058] The molecular weights of rose polysaccharide from rose waste liquid, rose polysaccharide fraction 1 from rose waste liquid, and rose polysaccharide fraction 2 from rose waste liquid were 1.25×10 5 ,7.15×10 3 and 3.82×10 4 g / mol.

[0059] Example 3: Hydroxyl radical scavenging experiment

[0060] The rose polysaccharide and its components (1 and 2) in Example 1 were prepared into a mother solution, and then a series of polysaccharide sample solutions of different concentrations (200, 400, 600, 800, 1000, 1500, 2000 mg / L) were prepared, and Vc solutions of equal concentration were prepared at the same time. 1 mL of ferrous sulfate solution (9 mmol / L), 1 mL of salicylic acid ethanol solution (9 mmol / L), 1 mL of polysaccharide sample solution and 1 mL of hydrogen peroxide solution (8.8 mmol / L) were added in sequence and mixed evenly. After 30 min in a 37°C water bath, the mixture was centrifuged at 6000 r / min for 10 min. The absorbance of the supernatant was measured at 510 nm and zeroed with deionized water. The hydroxyl radical scavenging rate (SR) was calculated by the formula. SR (%) = (A0-A) / A0×100. Wherein: A0 is the absorbance of the blank control, and A is the absorbance of the sample.

[0061] Within the experimental concentration range, the hydroxyl radical scavenging rate of rose polysaccharide and its components 1 and 2 showed a significant dose-effect relationship with the polysaccharide concentration. Rose polysaccharide and its components 1 and 2 all have good hydroxyl radical scavenging ability. According to EC 50 The scavenging rate of hydroxyl radicals at 2000 mg / L and 2000 mg / L showed that the hydroxyl radical scavenging capacity of rose polysaccharide and its components 1 and 2 was: rose polysaccharide component 2> rose polysaccharide component 1> rose polysaccharide.

[0062] Embodiment 4:

[0063] Rose polysaccharide and its components (1 and 2) were prepared into a mother solution, and then a series of polysaccharide sample solutions with different concentrations (200, 400, 600, 800, 1000, 1500, 2000 mg / L) were prepared, and Vc solutions of equal concentration were prepared at the same time. 0.2 mL of polysaccharide sample solution or Vc was taken into a test tube, and 2 mL of P solution (containing 0.6 mol / L sulfuric acid, 28 mmol / L trisodium phosphate, 4 mmol / L ammonium molybdate) was added, and the mixture was placed in a 95°C water bath for 90 min. After cooling, the absorbance was measured at 695 nm.

[0064] The principle of total antioxidant capacity determination is that antioxidants can reduce Fe 3+ Produces blue Fe 2 +Then, the total antioxidant capacity of the sample can be obtained by measuring the absorbance of the solution at 695nm. Figure 4 It can be seen that within the experimental concentration range, the total antioxidant capacity of rose polysaccharides and their components (1 and 2) increases with the increase of concentration. The order of total antioxidant capacity is: rose polysaccharide component 2> rose polysaccharide> rose polysaccharide component 1.

[0065] Embodiment 5:

[0066] Healthy AB wild-type zebrafish at 21 hpf (hours post fertilization) were used as experimental animal models to study the effects of different concentrations of sample solutions on melanin production. (NC) blank control group: fish water; (PC) positive control group: fish water containing 20 mg / mL arbutin; (I, II, III) rose waste liquid rose polysaccharide component 2 intervention groups: fish water containing 1, 10, 20 μg / mL rose waste liquid rose polysaccharide components. Wrap with tin foil and place in a light incubator (28°C) to allow the embryos to continue to develop for 24 hours. After 24 hours, observe the melanin production under a microscope and take pictures, and calculate the melanin area with Image-Pro Plus software.

[0067] Depend on Figure 5 It can be seen that within the experimental concentration range, as the sample concentration increases, the melanin in the zebrafish gradually decreases. When the sample concentration is 10 and 20 μg / mL, there is a very significant difference compared with the blank group (p<0.01), and the positive control group has a very significant difference compared with the blank group (p<0.01). When the concentration of the rose polysaccharide component is 20 μg / mL, the melanin area is reduced by 51.26% compared with the blank group. Therefore, a certain concentration of rose polysaccharide components can inhibit the production of melanin and play a role in whitening and lightening spots in the body.

[0068] Embodiment 6:

[0069] Healthy AB wild-type zebrafish at 21 hpf (hours post fertilization) were used as experimental animal models to study the effects of different concentrations of sample solutions on melanin production. (NC) blank control group: fish water; (PC) positive control group: fish water containing 20 mg / mL arbutin; (I, II, III) rose waste liquid rose polysaccharide intervention groups: fish water containing 1, 10, 20 μg / mL rose polysaccharides, respectively. Wrap with tin foil and place in a light incubator (28°C) to allow the embryos to continue to develop for 24 hours. After 24 hours, observe the melanin production under a microscope and take pictures, and calculate the melanin area with Image-Pro Plus software.

[0070] Depend on Figure 6It can be seen that within the experimental concentration range, as the sample concentration increases, the melanin in the zebrafish gradually decreases. When the sample concentration is 10 and 20 μg / mL, there is a very significant difference compared with the blank group (p<0.01), and the positive control group is very significantly different from the blank group (p<0.01). When the concentration of rose polysaccharide in rose waste liquid is 20 μg / mL, the melanin area is reduced by 39.81% compared with the blank group. Therefore, a certain concentration of rose polysaccharide can inhibit the production of melanin and play a role in whitening and lightening spots in the body.

[0071] Embodiment 7:

[0072] Healthy Tg(krt4:NTR-hKitGR)cy17 zebrafish embryos with green fluorescence 24 hours after fertilization were selected, and 1 mg / mL protease was added to remove the membrane. The zebrafish after removal of the membrane were transferred to a 24-well plate and randomly divided into 5 groups, with 10 in each well. (VC) Normal control group: culture water. (MC) Oxidative stress model group: culture water containing metronidazole (10mmol / L). (PC) Positive control group: culture water containing metronidazole (10mmol / L) and vitamin C (25μg / mL). (I, II) Low and high concentration intervention groups of rose waste liquid rose polysaccharide fraction 2: culture water containing metronidazole (10mmol / L) and rose polysaccharide fraction 2 (100 or 200μg / mL). The 24-well plate containing zebrafish was placed in a constant temperature incubator at 28℃ and cultured for 24 hours. After the zebrafish were anesthetized with tricaine solution, they were photographed under a fluorescence microscope, and the number of fluorescent spots in the trunk was calculated using Image pro-plus software.

[0073] The skin cells of the zebrafish Tg(krt4:NTR-hKitGR)cy17 strain were marked with green fluorescence. Metronidazole caused oxidative damage to the zebrafish skin cells, cell death, and disappearance of the fluorescent spots. Figure 7 As shown, the number of skin fluorescent cells in the metronidazole group (MC) was significantly lower than that in the blank control group (VC) (P<0.01), and the metronidazole-induced zebrafish oxidative stress model was successfully established. The number of skin fluorescent points in group II treated with rose waste liquid rose polysaccharide fraction 2 increased by 252.78% (P<0.05) compared with the metronidazole group (MC). Therefore, rose waste liquid rose polysaccharide fraction 2 has an antioxidant effect in vivo.

[0074] Embodiment 8:

[0075] Healthy Tg(krt4:NTR-hKitGR)cy17 zebrafish embryos with green fluorescence 24 hours after fertilization were selected, and 1 mg / mL protease was added to remove the membrane. The zebrafish after removal of the membrane were transferred to a 24-well plate and randomly divided into 5 groups, with 10 in each well. (VC) Normal control group: culture water. (MC) Oxidative stress model group: culture water containing metronidazole (10mmol / L). (PC) Positive control group: culture water containing metronidazole (10mmol / L) and vitamin C (25μg / mL). (I) Rose waste liquid rose polysaccharide intervention group: culture water containing metronidazole (10mmol / L) and rose polysaccharide (150μg / mL). The 24-well plate containing zebrafish was placed in a constant temperature incubator at 28℃ for 24 hours. After the zebrafish were anesthetized with tricaine solution, they were photographed under a fluorescence microscope, and the number of fluorescent spots in the trunk was calculated using Image pro-plus software.

[0076] The skin cells of the zebrafish Tg(krt4:NTR-hKitGR)cy17 strain were marked with green fluorescence. Metronidazole caused oxidative damage to the zebrafish skin cells, cell death, and disappearance of the fluorescent spots. Figure 8 As shown, the number of skin fluorescent cells in the metronidazole group (MC) was significantly lower than that in the blank control group (VC), and the metronidazole-induced zebrafish oxidative stress model was successfully established. The number of skin fluorescent points in the rose waste liquid rose polysaccharide treatment group (I) increased by 127.52% compared with the metronidazole group (MC). Therefore, rose waste liquid rose polysaccharide has an antioxidant effect in vivo.

[0077] It should be noted that the above examples are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention is described in detail with reference to the given examples, those skilled in the art may modify or replace the technical solution of the present invention as needed without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. Application of rose polysaccharide in the preparation of cosmetics with whitening and antioxidant effects; The preparation method of rose polysaccharide at least comprises separating and obtaining rose polysaccharide from rose waste liquid; When rose polysaccharide is separated from rose waste liquid, the preparation method specifically comprises: S1. Add ethanol to the rose waste liquid, mix and let stand, collect the precipitate by centrifugation and dry to obtain a solid; S2, the solid is re-dissolved, protein is removed, and dried to obtain; In the step S1, The rose waste liquid is specifically rose boiling water produced during the extraction of rose essential oil; The ethanol is high-concentration ethanol, and the ethanol is ethanol with a concentration of 70% or more; The volume ratio of the rose waste liquid to ethanol is 1:1-5; The standing time is 1-36 h; The specific centrifugal conditions are 8000-12000 r / min for 5-20 min; The drying is carried out by drying or freeze-drying; Put the rose in a rose essential oil / rose water distillation device, add 1-5 times the mass volume ratio of water, the unit of mass volume ratio is g / mL, heat at 90-100 ° C for 1-5 h to obtain rose cooking residue, filter the rose cooking residue, and the filtrate is the rose waste liquid; In the step S2, The specific method for re-dissolving the solid comprises adding the solid to deionized water, wherein the mass volume ratio of the solid to the deionized water is 1:10-100, and the unit of the mass volume ratio is g / mL; The deproteinization method adopts the Sevag method; the drying is carried out by drying or freeze-drying; In order to further purify the obtained rose polysaccharide, column chromatography was used to separate and purify the rose polysaccharide; The rose polysaccharide is separated and purified by DEAE-52 cellulose ion exchange column chromatography, dialyzed and freeze-dried to obtain; at this time, the rose polysaccharide is purified into two main components, which are named rose waste liquid rose polysaccharide component 1 and rose waste liquid rose polysaccharide component 2 respectively; the rose waste liquid rose polysaccharide component 2 is used to prepare whitening and antioxidant cosmetics; The specific purification process of the above components is as follows: Water and 0.1, 0.3, and 0.5 mol / L NaCl solutions were used for elution in sequence, the flow rate of the eluent was 0.5-2 mL / min, 25-35 tubes of eluent were collected for each elution concentration, the volume of each tube was 0.5-4 mL, tubes 5-25 were collected, dialyzed and freeze-dried, which was the rose waste liquid rose polysaccharide component 1; tubes 35-55 were collected, dialyzed and freeze-dried, which was the rose waste liquid rose polysaccharide component 2.

2. The use according to claim 1, characterized in that Place rose flowers in a rose essential oil / rose water distillation device, add 2.5 times the mass volume ratio of water, the unit of mass volume ratio is g / mL, heat at 100℃ for 3 h to obtain rose cooking residue, filter the rose cooking residue, and the filtrate is the rose waste liquid.

3. The use according to claim 1, characterized in that The ethanol is 95% ethanol.

4. The use according to claim 1, characterized in that The volume ratio of the rose waste liquid to ethanol is 1:

3.

5. The use according to claim 1, characterized in that Water and 0.1, 0.3, and 0.5 mol / L NaCl solutions were used for elution in sequence, the flow rate of the eluent was 1 mL / min, 30 tubes of eluent were collected for each elution concentration, the volume of each tube was 2 mL, tubes 5-25 were collected, dialyzed and freeze-dried, which was the rose waste liquid rose polysaccharide component 1; tubes 35-55 were collected, dialyzed and freeze-dried, which was the rose waste liquid rose polysaccharide component 2.

6. A cosmetic with whitening and anti-oxidation effects, characterized in that: The cosmetic comprises at least the rose polysaccharide used according to any one of claims 1 to 5.

7. The cosmetic according to claim 6, characterized in that The cosmetics include cleansing cream, facial cleanser, eye cream, shampoo, conditioner, hand soap, hand cream, body lotion, bath gel, facial cream, lotion and facial mask.

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