A high-density array liquid-adding nozzle module

Abstract

A high-density array-type liquid-adding nozzle module belongs to the field of DNA recombination technology, and includes a mounting plate, the upper end face of the mounting plate is bolted to an assembly plate, and a plurality of liquid-adding needles are arranged in an array on the assembly plate, and the lower ends of the plurality of liquid-adding needles extend to the lower end face of the mounting plate; the lower end face of the assembly plate is arrayed with a plurality of empty slots, and the empty slots are equipped with elastic pressure plates for locking the liquid-adding needles, and unlocking holes are provided through the upper end walls of the empty slots; firstly, the purpose of small size is achieved through modularization, and later expansion and maintenance are facilitated. In addition, the plurality of liquid-adding needles are assembled on a nozzle module to realize a multi-channel liquid-adding method and improve the liquid-adding efficiency. At the same time, combined with the locking of the elastic pressure plate, the unlocking and locking of a single liquid-adding needle are realized to facilitate disassembly and replacement.

Description

Technical Field

[0001] The invention belongs to the technical field of DNA recombination, and in particular relates to a high-density array type liquid adding nozzle module. Background Art

[0002] At present, artificially synthesized DNA is the only known way to modify gene sequences in a targeted manner. It is widely used in many fields such as protein modification and life sciences, such as nucleic acid drugs, enzyme engineering, genetic testing, and gene therapy.

[0003] The vast majority of DNA synthesizers on the market currently use the phosphite-amidite chemical synthesis method for DNA synthesis. Generally, this method requires injecting 10 different chemical reagents onto a porous glass synthesis carrier to perform steps such as deprotection, activation, linking, capping, and oxidation. The four monomer reagents, T, G, C, and A, are the primary raw materials for DNA synthesis. The activation reagent is ACT, and the capping reagents are CAPA and CAPB. The cleaning reagent is primarily acetonitrile solution (ACN), the oxidizing reagent is primarily iodine solution (OXI), and the deprotection reagent is primarily trichloroacetic acid solution (TCA). Ten reagents need to be injected into a synthesis plate containing synthesis carriers in a specific order according to specific chemical reaction steps. Usually, multi-well synthesis plates are usually arranged in 8×12 wells or 16×24 wells. During the operation of the DNA synthesizer, the reagents for each step of the reaction need to be injected into every well of the synthesis plate to ensure that all synthesis carriers are fully in contact with the reagents for chemical reaction. However, there are too many wells on the synthesis plate. If a single needle is used to inject the reagent into each well, it will be too time-consuming. Especially for high-throughput synthesizers, high efficiency and short time are particularly important.

[0004] The nozzle modules of synthesis instruments on the market are limited by the size of tubes, joints, and nozzles. The array density is large, >9mmx9mmm / nozzle. In high-throughput synthesis nozzles, the nozzle array size of 1,000 or 10,000 is too large, which limits the development of high-throughput instruments. For this reason, the existing nozzles are basically an integrated structure. In the event of damage, the entire nozzle needs to be replaced separately. Summary of the Invention

[0005] The object of the present invention is to provide a high-density array type liquid adding nozzle module to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A high-density array-type liquid-adding nozzle module includes a mounting plate, the upper end face of which is bolted to an assembly plate, a plurality of liquid-adding needle tubes are arranged in an array on the assembly plate, and the lower ends of the plurality of liquid-adding needle tubes extend to the lower end face of the mounting plate; a plurality of empty slots are arranged in an array on the lower end face of the assembly plate, and elastic pressure plates for locking the liquid-adding needle tubes are built into the empty slots, and an unlocking hole is provided through the upper end wall of the empty slot.

[0008] Compared with the existing technology, this technical solution has the following effects:

[0009] First, modularization is used to achieve the goal of small size and facilitate subsequent expansion and maintenance. In addition, several dosing needles are assembled on a nozzle module to achieve a multi-channel dosing method and improve the dosing efficiency. At the same time, combined with the locking of the elastic pressure plate, the unlocking and locking of a single dosing needle can be achieved for easy disassembly and replacement.

[0010] Preferably, the size of the mounting plate is larger than that of the assembly plate.

[0011] Preferably, the liquid-filling needle tube includes a first tube body, a second tube body is inserted into the lower end of the first tube body, and a clamping member is sleeved on the outer side of the lower end of the first tube body.

[0012] Preferably, the internal array of the empty slots is provided with a plurality of partition plates which divide the empty slots into a plurality of areas, and the plurality of areas are interconnected.

[0013] Preferably, the elastic pressure plate comprises a plate body, a plurality of pressing blocks are arranged in an array on the plate body, the plurality of pressing blocks correspond to the plurality of regions one-to-one, and the plurality of pressing blocks are elastically connected to the plate body.

[0014] Preferably, an unlocking hole is provided on the unlocking end of the elastic pressure plate extending upward, and the unlocking hole is communicated with the empty slot.

[0015] Preferably, the assembly plate body is provided with an assembly hole for inserting a single liquid-dosing needle tube, and the main body of the mounting plate is provided with an insertion hole for inserting a single liquid-dosing needle tube, and the assembly hole and the insertion hole are coaxially arranged.

[0016] Preferably, the inner diameter of the assembly hole is larger than the inner diameter of the insertion hole.

[0017] Preferably, the clamping member is accommodated in the insertion hole. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0019] Figure 2 This is the first exploded schematic diagram of the overall structure of the present invention;

[0020] Figure 3 This is a second exploded schematic diagram of the overall structure of the present invention;

[0021] Figure 4 yes Figure 2 Schematic diagram of the overall structure in the "A" direction;

[0022] Figure 5 It is a schematic diagram of the overall structure of the elastic member in the present invention.

[0023] In the figure: 1. Mounting plate; 10. First mounting hole; 11. Insertion hole; 12. Second mounting hole; 2. Assembly plate; 20. Third mounting hole; 21. Unlocking hole; 22. Empty slot; 23. Partition plate; 24. Assembly hole; 3. Dosing needle; 30. First tube body; 31. Clamping piece; 32. Second tube body; 4. Elastic pressure plate; 40. Plate body; 41. Connecting piece; 42. Connecting rod; 43. Pressing block; 430. Arc surface. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0025] Example:

[0026] like Figure 1 The high-density array-type liquid-adding nozzle module shown includes a mounting plate 1, the upper end face of the mounting plate 1 is bolted to an assembly plate 2, and liquid-adding needle tubes 3 are arranged in an array on the assembly plate 2, and the lower ends of the liquid-adding needle tubes 3 pass through the lower end face of the mounting plate 1; wherein, the mounting plate 1 is used to be installed on a movable component to realize the adjustable position of the liquid-adding nozzle module.

[0027] It is worth mentioning that the problem of small-sized overall difficulty in processing is solved by changing the liquid-filling needle tube 3 from a traditional integrated structure to the existing segmented structure, as follows: the liquid-filling needle tube 3 includes a first tube body 30, and a second tube body 32 is inserted into the lower end of the first tube body 30, and a clamping member 31 is mounted on the outer side of the lower end of the first tube body 30. In this embodiment, the clamping member 31 is made of metal, and through the clamping member 31, on the one hand, the second tube body 32 is fastened to the lower end of the first tube body 30 to achieve a sealing effect; on the other hand, it is combined with the mounting plate 1 to achieve its limiting purpose.

[0028] Combine Figure 2-4, the specific structures of the mounting plate 1 and the assembly plate 2 are elaborated to explain how to achieve position limiting. The mounting plate 1 includes a mounting plate body, the mounting plate body is provided with a first mounting hole 10 for mounting the mounting plate body on the moving component, and the mounting plate body is provided with two rows of second mounting holes 12 spaced apart from each other in front and back. An array of through holes 11 is provided between the two rows of second mounting holes 12, and the holding member 31 is accommodated in the through holes 11;

[0029] The assembly plate 2 includes an assembly plate body, and two rows of third mounting holes 20 are arranged on the assembly plate body at intervals from front to back. The two rows of third mounting holes 20 correspond to the two rows of second mounting holes 12 located on the assembly plate body. That is, according to the direction definition in this embodiment, the third mounting holes 20 and the second mounting holes 12 are overlapped when projected from top to bottom, and an assembly hole 24 is arranged in an array at the interval between the two rows of third mounting holes 20. The tube body of the liquid-filling needle tube 3 is accommodated in the assembly hole 24. In this embodiment, an unlocking hole 21 is provided on one side of the assembly hole 24 (the so-called right side), and an empty slot 22 is arranged in an array on the lower end surface of the assembly plate body. A partition plate 23 is arranged in an array inside the empty slot 22 to divide the interior of the empty slot 22 into several areas. Each of the several areas corresponds to a lock hole group (the so-called lock hole group is a combination of an assembly hole 24 + an unlocking hole 21). Figure 3 It can be seen that several areas are connected to each other. More specifically, the width of the partition plate 23 is smaller than the width of the empty slot 22. The purposes of adopting this setting are as follows: first, it is relatively easy to process and more economical in manufacturing cost; second, it provides convenience for the subsequent disassembly, assembly and replacement of a single liquid-filling needle 3.

[0030] How to facilitate the disassembly, assembly, and replacement of a single dosing needle 3 is described in detail with reference to the relevant structures in a specific embodiment. In this embodiment, an elastic pressure plate 4 is built into the empty groove 22 to secure the portion of the dosing needle 3 accommodated in the empty groove 22.

[0031] In this embodiment, combined with Figure 5It can be seen that the elastic pressure plate 4 includes a plate body 40, and the upper end fold of the plate body 40 is provided with a mounting end, and the mounting end array is provided with a plurality of connecting members 41, and the plurality of connecting members 41 are fixedly connected to a connecting rod 42, and the lower end of the connecting rod 42 is laterally provided with a pressing block 43, that is, each area separated by a plurality of partition plates 23 is provided with a pressing block 43, so that the elastic pressure plate 4 is more stably installed in the empty groove 22. At the same time, the abutting end surface of the pressing block 43 is provided with an arc surface 430, and the arc surface 430 is provided to better contact the outer wall of the tube body of the liquid-filling needle tube 3, so that the installation of the liquid-filling needle tube 3 is convenient. More stable. Of course, in order to further enhance the practicality of the device, a flexible layer may be provided on the curved surface 430 to prevent the pressure generated by the abutment from damaging the tube body of the dosing needle tube 3. It should be noted that the plate body 40, the connector 41, the connecting rod 42 and the pressing block 43 are all made of metal, and the four are an integrated structure. In a specific application scenario, when disassembling and assembling a single dosing needle tube 3, a tool is used to press the connecting rod 42 through the unlocking hole 21 to separate the curved surface 430 on the pressing block 43 from the outer wall of the dosing needle tube 3. At this time, the user pulls up the single dosing needle tube 3 to replace the single one.

[0032] In the description of the present invention, it should be understood that the terms "center", "lateral", "up", "down", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "several" means two or more. In addition, the term "including" and any variations thereof are intended to cover non-exclusive inclusions.

[0033] The present invention has been described in accordance with the embodiments. Without departing from the principles herein, the present invention may be modified and improved in several ways. It should be noted that any technical solution obtained by equivalent replacement or equivalent transformation falls within the scope of protection of the present invention.

Claims

1. A high-density array type liquid-adding nozzle module, comprising a mounting plate (1), the mounting plate being used to be mounted on a moving assembly to achieve adjustable position of the liquid-adding nozzle module, the upper end surface of the mounting plate (1) being bolted to an assembly plate (2), a plurality of liquid-adding needle tubes (3) being arranged in an array on the assembly plate (2), the lower ends of the plurality of liquid-adding needle tubes (3) extending to the lower end surface of the mounting plate (1); characterized in that: The lower end surface of the assembly plate (2) is provided with a plurality of empty slots (22) in an array, wherein the empty slots (22) are provided with elastic pressing plates (4) for locking the liquid-adding needle tubes (3), and the upper end walls of the empty slots (22) are provided with unlocking holes (21); The internal array of the empty slot (22) is provided with a plurality of partition plates (23) for dividing the empty slot (22) into a plurality of areas, and the plurality of areas are interconnected; The elastic pressure plate (4) includes a plate body (40), and a plurality of pressing blocks (43) are arranged in an array on the plate body (40), the plurality of pressing blocks (43) correspond to the plurality of areas one by one, and the plurality of pressing blocks (43) are elastically connected to the plate body (40); An unlocking hole (21) is provided on the unlocking end of the elastic pressure plate (4) extending upward, and the unlocking hole (21) is communicated with the empty slot (22).

2. A high-density array liquid-adding nozzle module according to claim 1, characterized in that: The size of the mounting plate (1) is larger than that of the assembly plate (2).

3. The high-density array liquid-adding nozzle module according to claim 1, characterized in that: The liquid-adding needle tube (3) comprises a first tube body (30), a second tube body (32) is inserted into the lower end of the first tube body (30), and a clamping member (31) is sleeved on the outer side of the lower end of the first tube body (30).

4. A high-density array liquid-adding nozzle module according to claim 3, characterized in that: The assembly plate (2) is provided with an assembly hole (24) for inserting a single liquid-adding needle tube (3), and the main body of the installation plate (1) is provided with an insertion hole (11) for inserting a single liquid-adding needle tube (3), and the assembly hole (24) and the insertion hole (11) are coaxially arranged.

5. The high-density array liquid-adding nozzle module according to claim 4, characterized in that: The inner diameter of the assembly hole (24) is larger than the inner diameter of the insertion hole (11).

6. A high-density array liquid-adding nozzle module according to claim 4 or 5, characterized in that: The holding member (31) is accommodated in the insertion hole (11).

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