An automatic detection system

Abstract

The application provides an automatic detection system, which comprises a material plate, a droplet generation device, an amplification device, a sampling device, an optical detection device, a conveying device and a power supply device, the material plate is loaded with a first sample, the droplet generation device is used for electric treatment on the first sample to obtain a second sample, the amplification device is used for temperature control treatment on the second sample to obtain a third sample, the sampling device is used for obtaining the material plate and conveying the third sample, the optical detection device is used for receiving the third sample and projecting light on the third sample for detection, the conveying device is used for conveying the material plate between the droplet generation device, the amplification device and the sampling device, and the power supply device is used for providing power for the droplet generation device, the amplification device, the sampling device, the optical detection device and the conveying device, so that automatic sampling and detection operation are realized, human intervention is reduced, the detection efficiency is improved, and the risk of pollution caused by misoperation is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medical equipment, in particular to an automatic detection system. BACKGROUND

[0002] Extraction of bioactive substances, such as cells, and protein, nucleic acid and other bioactive substances, has important roles in modern clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, molecular biology research and other fields. Among them, nucleic acid is a bioactive substance compound polymerized by many nucleotides, and is one of the most basic substances of life. Nucleic acid widely exists in all animal, plant cells, microorganisms and organisms. According to the different chemical compositions, nucleic acid can be divided into ribonucleic acid (abbreviated as RNA) and deoxyribonucleic acid (abbreviated as DNA). DNA is the main material basis for storing, copying and transmitting genetic information, and RNA plays an important role in the process of protein synthesis. The development of biotechnology is changing with each passing day, and with the application of PCR technology in various fields, including medical disease detection, agricultural transgenic detection and other applications.

[0003] Therefore, in the existing nucleic acid detection method, the nucleic acid detection is basically carried out in a real-time fluorescence quantitative manner, and the effect of detecting nucleic acid by this manner usually has a large error, and the throughput is usually small, so that it is not possible to detect a large number of samples at the same time, and manual replacement of samples is required, which greatly increases the human workload and increases the risk of pollution. SUMMARY

[0004] The main purpose of the present application is to solve the problems of the prior art, and to provide an automatic detection system.

[0005] The present application provides an automatic detection system in a first aspect, which comprises:

[0006] A material plate, wherein the first sample is loaded in the material plate;

[0007] A droplet generation device for performing electrical treatment on the first sample on the material plate to obtain a second sample;

[0008] An amplification device for performing temperature control treatment on the second sample on the material plate to obtain a third sample;

[0009] A sampling device for receiving and conveying the third sample after temperature control treatment;

[0010] An optical detection device for receiving the third sample conveyed by the sampling device and projecting light on the third sample for detection work;

[0011] a conveying device for conveying the substrate between the droplet generation device, the amplification device, and the sampling device;

[0012] a power supply device for supplying power to the droplet generation device, the amplification device, the sampling device, the optical detection device, and the conveying device.

[0013] Preferably, the droplet generation device comprises a base plate, a support frame mounted on the base plate, a first substrate table for placing the substrate, and a driving member mounted on the support frame, the first substrate table is mounted on the base plate and placed in the support frame, and a processing assembly for electrically processing the first sample is arranged on the output end of the driving member, wherein the processing assembly is driven by the driving member to approach or move away from the substrate.

[0014] Preferably, the processing assembly comprises an electrospray box mounted on the output end of the driving member and a plurality of electrode needles mounted on the side of the electrospray box away from the driving member.

[0015] Preferably, the sampling device comprises a second substrate table for placing the substrate, a first frame body, a first three-axis driver mounted on the first frame body, and a sampling assembly drivingly connected with the first three-axis driver, the sampling assembly is used to obtain the third sample in the substrate from the second substrate table and transmit the third sample to the optical detection device, and the first three-axis driver is used to control the movement of the sampling assembly in a three-axis space.

[0016] Preferably, the sampling assembly comprises a mounting block drivingly connected with the first three-axis driver and a sampling needle for obtaining the third sample, a cleaning cavity and a mounting hole communicating with the cleaning cavity are arranged in the mounting block, first and second holes are respectively arranged on the two sides of the cleaning cavity adjacent to the mounting hole, a first end of the sampling needle sequentially passes through the first hole, the cleaning cavity, and the second hole, and a second end of the sampling needle is connected with the optical detection device.

[0017] Preferably, the second hole has a larger hole diameter than the first end of the sampling needle.

[0018] Preferably, the optical detection device comprises:

[0019] an emitter for emitting a plurality of light beams of different wavelengths;

[0020] an optical path adjuster for receiving the light beams of the emitter, the optical path adjuster is provided with a capillary tube connected with the second end of the sampling needle, and the optical path adjuster converges the received light beams in the capillary tube to form a light spot;

[0021] a receiver for receiving the light spot.

[0022] Preferably, the light path adjuster comprises:

[0023] A second three-axis driver;

[0024] A first light path opened on the second three-axis driver;

[0025] A second frame body, the second frame body being provided with an adjusting rail and a first fixing block;

[0026] A second fixing block slidably mounted on the adjusting rail;

[0027] The second three-axis driver is used to control the irradiation position of the light beam guided by the first light path, and the first fixing block and the second fixing block fix the capillary at the light outlet of the first light path.

[0028] Preferably, the optical detection device further comprises a light intensity detector for detecting the intensity of the light beam emitted by the emitter, and the light path adjuster further comprises a second light path mounted on the second three-axis driver, the second light path being used to receive the light beam emitted by the emitter and guide the light beam to irradiate on the light intensity detector.

[0029] Preferably, the optical detection device further comprises an adjusting block movably mounted on the second frame body and a condenser lens mounted on the adjusting block.

[0030] The present application has the beneficial effect of providing an automatic detection system, which comprises a material plate, a droplet generation device, an amplification device, a sampling device, an optical detection device, a conveying device and a power supply device, the material plate being loaded with a first sample, the droplet generation device being used to perform electrical treatment on the first sample on the material plate to obtain a second sample, the amplification device being used to perform temperature control treatment on the second sample on the material plate to obtain a third sample, the sampling device being used to obtain and convey the third sample on the material plate, the optical detection device being used to receive the third sample and project light on the third sample for detection work, the conveying device being used to convey the material plate between the droplet generation device, the amplification device and the sampling device, and the power supply device being used to provide power for the droplet generation device, the amplification device, the sampling device, the optical detection device and the conveying device, so as to realize automatic sampling operation and detection operation, reduce human intervention, improve detection efficiency, and reduce the risk of pollution caused by misoperation. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 The structure schematic diagram of the embodiment in the present application;

[0032] Figure 2 The structure schematic diagram of the droplet generation device in the present application;

[0033] Figure 3 This is a schematic diagram of the sampling device in this invention;

[0034] Figure 4 In this invention Figure 3 A schematic diagram of the sampling needle and mounting block;

[0035] Figure 5 for Figure 4 Sectional view of section AA;

[0036] Figure 6 for Figure 5 Enlarged view of part B in the middle;

[0037] Figure 7 This is a schematic diagram of the optical detection device in this invention;

[0038] Figure 8 This is a schematic diagram of the optical path adjuster in this invention. Figure 1 ;

[0039] Figure 9 This is a schematic diagram of the optical path adjuster in this invention. Figure 2 ;

[0040] Figure 10 for Figure 9 A cross-sectional view of the central CC section.

[0041] Table of labels in the diagram:

[0042]

[0043]

[0044] Detailed Implementation

[0045] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions of the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this disclosure. All other embodiments obtained by those skilled in the art based on the described embodiments of this disclosure without creative effort are within the scope of protection of this disclosure.

[0046] Unless otherwise defined, the technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this disclosure pertains.

[0047] The terms "first," "second," and similar words used in this patent application specification and claims do not indicate any order, quantity, or importance, but are merely used to distinguish different components. The terms "comprising" or "including," and similar words, mean that the elements or objects preceding "comprising" or "including" encompass the elements or objects listed following "comprising" or "including," and do not exclude other elements or objects. The terms "connected" or "linked," and similar words, are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. "Above," "below," "left," "right," etc., are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0048] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0049] See appendix Figures 1-10 The present invention provides an automatic detection system in a first aspect, comprising: a material plate 100, wherein a first sample is loaded in the material plate 100;

[0050] Droplet generating apparatus 200 for electrically processing a first sample on a material plate 100 to obtain a second sample;

[0051] Amplification device 300 for temperature-controlled processing of the second sample on the material plate 100 to obtain the third sample;

[0052] Sampling device 400 for receiving and transporting a third sample after temperature control treatment;

[0053] An optical detection device 500 is used to receive the third sample delivered by the sampling device 400 and project light onto the third sample for detection.

[0054] A conveying device 600 for transporting the material plate 100 between the droplet generating device 200, the amplification device 300, and the sampling device 400;

[0055] A power supply device 700 for providing power to the droplet generation device 200, the amplification device 300, the sampling device 400, the optical detection device 500, and the conveying device 600.

[0056] Specifically, the automatic detection system adopts automation to realize the transfer and detection of the sample. The conveying device 600 is a three-axis grabbing device. As can be seen from the drawings, the conveying device 600 includes a transverse track across the droplet generation device 200, the amplification device 300 and the sampling device 400, so that the grabbing structure for grabbing the substrate 100 on the conveying device 600 can convey the substrate 100 between the droplet generation device 200, the amplification device 300 and the sampling device 400. Since the structures of the three devices are different, the positions for placing the substrate 100 are also different. Therefore, the conveying device 600 is further provided with a longitudinal track and a vertical track structure to facilitate grabbing or placing the substrate 100.

[0057] Further, the droplet generation device 200 includes a bottom plate 201, a support frame 202 mounted on the bottom plate 201, a first substrate placing table 203 for placing the substrate 100, and a driving member 204 mounted on the support frame 202. The first substrate placing table 203 is mounted on the bottom plate 201 and placed in the support frame 202. The output end of the driving member 204 is provided with a processing assembly 205 for electrically processing the first sample. Under the drive of the driving member 204, the processing assembly 205 approaches the substrate 100 and electrically processes the first sample in the substrate 100 or moves away from the substrate 100. The processing assembly 205 includes an electrospray box 2051 mounted on the output end of the driving member 204 and a plurality of electrode needles 2052 mounted on the side of the electrospray box 2051 away from the driving member 204.

[0058] Specifically, in the present embodiment, the material plate 100 is a 96-well plate, and the number of electrode needles 2052 is also 96. When the conveying device 600 places the material plate 100 loaded with the first sample on the first material placing table 203 of the droplet generating device 200, the driving member 204 is activated to drive the electro-spray box 2051 and the electrode needles 2052 to approach the material plate 100. The driving member 204 is a combination of a driving motor and a plurality of transmission structures, which can drive the electro-spray box 2051 and the electrode needles 2052 to approach the material plate 100. The 96 electrode needles 2052 are electrically connected to the electro-spray box 2051. It should be noted that the height of the first material placing table 203 is fixed, and the material plate 100 is also of a uniform specification. After the material plate 100 is placed on the first material placing table 203, the total height position can be calculated, so the stroke of the driving member 204 driving the electro-spray box 2051 and the electrode needles 2052 to approach the material plate 100 is fixed, and the electrode needles 2052 can be driven to enter the holes of the material plate 100 to contact the first sample. At this time, the electrode needles 2052 discharge to generate electro-spray, and after a period of time, the sample droplet is generated to obtain the second sample. The driving member 204 drives the electro-spray box 2051 and the electrode needles 2052 to move away from the material plate 100, so that the electrode needles 2052 are separated from the holes of the material plate 100. It should be noted that the time is determined according to the actual production requirement or the sample droplet production time. At this time, the conveying device 600 transfers the material plate 100 loaded with the second sample to the amplification device 300.

[0059] The amplification device 300 provides temperature rising and falling cycles for the second sample. Through the change of temperature, the second sample is cracked to obtain the third sample to meet the final detection requirement. In order to prevent the second sample from being affected by external factors during amplification and the third sample from being cross-contaminated or infected by the environment during the transfer process, the material plate 100 loaded with the second sample is sealed before the amplification device 300 controls the temperature and amplifies the second sample. After the sealing, temperature control and amplification, the conveying device 600 transfers the material plate 100 loaded with the third sample to the sampling device 400.

[0060] The specific structure of the amplification device 300 is not shown in the drawings, but the structure of the amplification device 300 is briefly described as follows: a temperature-variable seat for placing the substrate 100, a heat-insulating cover that can be lifted and covers the substrate 100, and an aluminum film. The temperature-variable seat is provided with a plurality of heating and cooling elements (such as Peltier elements) that can be heated or cooled by changing the state of the current. After the substrate 100 is placed on the temperature-variable seat, the heat-insulating cover covers the substrate 100, so that the substrate 100 can be heated uniformly. The aluminum film also covers the plurality of holes of the substrate 100 and is heated to seal the substrate 100. At this time, the substrate 100 is subjected to cyclic changes of heating and cooling, and the amplification work is completed when the second sample in the substrate 100 is amplified to the standard requirement of the third sample.

[0061] Further, the sampling device 400 includes a second substrate placing table 404 for placing the substrate 100, a first frame body 401, a first three-axis driver 402 mounted on the first frame body 401, and a sampling assembly 403 drivenly connected with the first three-axis driver 402. The sampling assembly 403 is used to obtain the third sample in the substrate 100 from the second substrate placing table 404 and transmit the third sample to the optical detection device 500. The first three-axis driver 402 is used to control the movement of the sampling assembly 403 in a three-axis space.

[0062] Referring to the drawings Figure 8 - the drawings Figure 10 The first three-axis driver 402 is essentially a three-axis space driving device formed by stacking three driving shafts. The sampling assembly 403 is mounted on the driving shaft of the first three-axis driver 402 in the vertical direction.

[0063] Further, the sampling assembly 403 further includes a mounting block 4031 drivenly connected with the first three-axis driver 402 and a sampling needle 4036 used to obtain the third sample. The mounting block 4031 is provided with a cleaning cavity 4032 and a mounting hole 4033 connected with the cleaning cavity 4032. The mounting hole 4033 is open to the outside of the mounting block 4031 away from the cleaning cavity 4032. The two side surfaces adjacent to the cleaning cavity 4032 and the mounting hole 4033 are respectively provided with a first hole 4034 and a second hole 4035. The first end of the sampling needle 4036 passes through the first hole 4034, the cleaning cavity 4032, and the second hole 4035 in sequence. The second end of the sampling needle 4036 is connected with the optical detection device 500. The aperture of the second hole 4035 is larger than the pipe diameter of the first end of the sampling needle 4036.

[0064] Specifically, it is worth noting that the second end of the sampling needle 4036 is connected with a medical water pump, and the third sample sucked by the first end of the sampling needle 4036 will enter the optical detection device 500 from the second end of the sampling needle 4036 along the pipeline of the sampling needle 4036 through the extraction of the medical water pump. Since the material plate 100 placed on the second material table 404 is in a sealed state, the first end of the sampling needle 4036 will pierce the aluminum film and extend into the corresponding hole of the material plate 100 to suck the third sample under the drive of the first three-axis driver 402. After completing the sampling work, the first end of the sampling needle 4036 needs to be cleaned to prevent the sampling needle 4036 from being contaminated by the sample remaining on the sampling needle 4036 for the next sampling work. The mounting hole 4033 is used to install an external device containing cleaning liquid, and the mounting hole 4033 is connected in a threaded manner to facilitate disassembly and replacement. The device containing cleaning liquid introduces cleaning liquid into the cleaning cavity 4032. Since the aperture of the second hole 4035 is larger than the pipe diameter of the first end of the sampling needle 4036, the cleaning liquid flows out along the outer wall of the needle tube of the sampling needle 4036 to clean the outer wall of the first end of the sampling needle 4036.

[0065] Further, the optical detection device 500 comprises:

[0066] an emitter 501 for emitting a plurality of light beams of different wavelengths;

[0067] an optical path adjuster 502 for receiving the light beams of the emitter 501, the optical path adjuster 502 being provided with a capillary tube 503 connected with the second end of the sampling needle 4036, wherein the optical path adjuster 502 collects the received light beams in the capillary tube 503 to form a light spot;

[0068] a receiver 504 for receiving the light spot.

[0069] In the embodiment, the emitter 501 is composed of four light emitting elements respectively emitting light beams of different wavelengths, and the light beams emitted by the four light emitting elements are collected by a filter to form integrated light, and the integrated light is irradiated into the optical path adjuster 502. The optical path adjuster 502 guides and irradiates the integrated light to the capillary tube 503 to form a light spot, and the receiver 504 is provided with a PMT corresponding to receiving the four different wavelengths of light beams and corresponding to outputting a detection signal.

[0070] Further, the optical path adjuster 502 comprises:

[0071] a second three-axis driver 5021;

[0072] The first light path 5022 is installed on the second three-axis driver 5021, and is used to receive the light beam emitted by the emitter 501 and guide the light beam to irradiate on the capillary tube 503 to form a light spot. The second three-axis driver 5021 is used to control the irradiation position of the light beam guided by the first light path 5022.

[0073] The second frame body 5023 is provided with an adjusting rail 5024 and a first fixing block 5025.

[0074] The second fixing block 5026 is slidably installed on the adjusting rail 5024. The first fixing block 5025 and the second fixing block 5026 fix the capillary tube 503 at the light outlet of the first light path 5022.

[0075] Specifically, the second three-axis driver 5021 has the same implementation effect as the first three-axis driver. However, in the embodiment, the second three-axis driver 5021 is composed of three block bodies fixedly connected through screws and adjusting grooves. When the corresponding adjustment position needs to be adjusted, the screws are loosened, the corresponding block body position is finely adjusted, and the screws are locked again to realize the driving control in the three-axis space. The fine adjustment distance is limited to the length of the adjusting groove.

[0076] The first light path 5022 receives the light beam of the emitter 501 and guides it to the capillary tube 503 in the embodiment. The structure is composed of multiple convex lenses, optical filters and optical mirrors. By finely adjusting the three block bodies, the focal length, the light refraction angle and the position of the final light spot falling on the capillary tube 503 can be controlled.

[0077] Further, the optical detection device 500 further comprises a light intensity detector 505 for detecting the light beam intensity emitted by the emitter 501. The light path adjuster 502 further comprises a second light path 5027 installed on the second three-axis driver 5021. The second light path 5027 is used to receive the light beam emitted by the emitter 501 and guide the light beam to irradiate on the light intensity detector 505.

[0078] Specifically, the second light path 5027 directly transmits the integrated light to the light intensity detector 505. The light intensity detector 505 is also provided with four windows for observing the light intensity. The four windows are provided with adjusting plates for adjusting the light intensity. The four windows are respectively provided with optical filters, so that the four windows correspondingly display four different wavelength light beams emitted by the emitter 501. In this way, it can be quickly determined which light body has too large or too small light intensity, and corresponding adjustment can be made.

[0079] Further, the optical detection device 500 further comprises an adjusting block 506 movably mounted on the second frame body 5023 and a condenser 507 mounted on the adjusting block 506, the condenser 507 is used to map the light spot in the receiver 504.

[0080] Specifically, since the integrated light falls on the capillary tube 503, the accurate position of the light spot cannot be determined, only the corresponding position can be roughly known, therefore, by adjusting the position of the condenser 507, the condenser 507 can be controlled to be aligned with the light spot, so that the receiver 504 can obtain clearer signals, wherein the adjusting block 506 can be a block body fixed on the second frame body 5023 by a screw, and the block body has a slot fixed with the screw, the specific structure is the same as the block body in the second three-axis driver 5021, however, this is only for controlling a single direction, similarly, the adjusting block 506 can also have the same structure as the second three-axis driver 5021, to realize the fine adjustment of the condenser 507 in the three-axis space, so that the position of the light spot is more accurate.

[0081] In the embodiment, the optical path adjuster 502 further comprises a slit mounting block, and a slot hole for mounting the slit mounting block is arranged on the second three-axis driver 5021, wherein the slot hole is located on the path of the first optical path 5022, when the slit mounting block is mounted in the slot hole, the size of the light spot of the integrated light finally presented on the capillary tube 503 can be controlled.

[0082] The above is only the embodiment of the present application, it should be pointed out that for those skilled in the art, without departing from the inventive concept, improvements can be made, but these all belong to the protection scope of the present application.

Claims

1. An automatic detection system, characterized in that, include: A material plate containing a first sample; A droplet generation device for electrically processing a first sample on the material plate to obtain a second sample; An amplification device for temperature-controlled processing of the second sample on the material plate to obtain the third sample; A sampling device for receiving and transporting a third sample after temperature control treatment; An optical detection device for receiving the third sample delivered by the sampling device and projecting light onto the third sample for detection. A conveying device for transporting the material plate between the droplet generating device, the amplification device, and the sampling device; A power supply device for providing power to droplet generation devices, amplification devices, sampling devices, optical detection devices, and conveying devices; The sampling device includes a sampling needle; The optical detection device includes: A transmitter used to emit beams of multiple different wavelengths; An optical path adjuster for receiving the beam of light from the transmitter is provided on the optical path adjuster and a capillary tube connected to the second end of the sampling needle is provided on the optical path adjuster, wherein the optical path adjuster focuses the received beam of light on the capillary tube to form a light spot; A receiver for receiving the light spot; The optical path modulator includes: Second and third axis drivers; The first optical path is established on the second and third axis drivers; The second frame is equipped with an adjustment rail and a first fixing block; A second fixing block that can be slidably mounted on the adjusting rail; The second three-axis driver is used to control the irradiation position of the beam guided by the first optical path, and the first fixing block and the second fixing block fix the capillary at the light outlet of the first optical path. The optical detection device further includes a light intensity detector for detecting the intensity of the light beam emitted by the transmitter, and the optical path adjuster further includes a second optical path mounted on the second three-axis driver. The second optical path is used to receive the light beam emitted by the transmitter and guide the light beam to illuminate the light intensity detector.

2. The automatic detection system according to claim 1, characterized in that, The droplet generating device includes: Base plate; Support frame mounted on the base plate; A first material placement platform for placing the material plate is mounted on a base plate and placed inside a support frame; The drive unit is mounted on the support frame, and the output end of the drive unit is provided with a processing component for electrically processing the first sample. Under the drive of the driving component, the processing component moves closer to or further away from the material plate.

3. The automatic detection system according to claim 2, characterized in that, The processing component includes: An electro-spray box installed on the output end of the drive unit; Multiple electrode needles are installed on the side of the electrospray box away from the drive unit.

4. The automatic detection system according to claim 1, characterized in that, The sampling device includes: A second material placement platform for placing the material plate; First frame; The first three-axis drive is mounted on the first frame; A sampling component is connected to the first three-axis driver and is used to acquire a third sample from the material plate on the second feeding stage and to transmit the third sample to the optical detection device. The first three-axis driver is used to control the movement of the sampling component in the three-axis space.

5. The automatic detection system according to claim 4, characterized in that, The sampling component includes: A mounting block is connected to the first three-axis driver. The mounting block has a cleaning chamber and a mounting hole that connects to the cleaning chamber. The cleaning chamber and the mounting hole are respectively provided with a first hole and a second hole on their two sides. A sampling needle for obtaining a third sample, the first end of which passes through the first hole, the cleaning chamber and the second hole in sequence, and the second end of which is connected to an optical detection device.

6. The automatic detection system according to claim 5, characterized in that, The diameter of the second hole is larger than the diameter of the first end of the sampling needle.

7. The automatic detection system according to claim 1, characterized in that, The optical detection device further includes an adjustment block movably mounted on the second frame and a condenser lens mounted on the adjustment block.

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