A method for promoting carotenoid accumulation in corn sprouts

Abstract

The present invention discloses a method for promoting carotenoid accumulation in corn sprouts. The method comprises the following steps: soaking corn seeds and then sowing them on a seed germination bed; spraying a 10-200 μM tryptamine solution on the corn seeds, and culturing them under light until the embryo stage to obtain corn sprouts. The method of the present invention synergistically treats the corn seeds with tryptamine and monochromatic light during germination. By controlling the concentration of tryptamine and the wavelength and intensity of the monochromatic light, the method effectively promotes carotenoid accumulation in the corn sprouts, thereby cultivating corn sprouts with a high total carotenoid content. The present invention provides theoretical support for the cultivation of high-value-added corn sprouts and has good economic benefits.

Description

Technical Field

[0001] The invention belongs to the technical field of food science, and particularly relates to a method for promoting the accumulation of carotenoids in corn sprouts. Background Art

[0002] Carotenoids are pigments widely distributed in nature, primarily found in the petals, leaves, and stems of plants. Carotenoids can be divided into oxygen-containing carotenoids (luteins) and non-oxygenated carotenoids (carotenes), depending on whether they contain oxygen. Among the xanthophylls, lutein and zeaxanthin are the primary pigments in the macular region of the human retina, playing a positive role in maintaining eye health and protecting vision. Furthermore, lutein's oxygen-containing properties give it antioxidant, free radical scavenging, and anti-cancer and anti-tumor properties. Among the carotenes, β-carotene is particularly important, serving as the primary source of vitamin A in the human body and providing a precursor for its synthesis. Therefore, simple and efficient cultivation methods for obtaining high levels of carotenoids are crucial for fully realizing the application value of corn sprouts.

[0003] Germination can effectively improve the nutritional quality and bioactive compound content of corn, particularly by increasing the carotenoid content in corn sprouts. Existing technologies for increasing the carotenoid content of corn sprouts typically involve NaCl stress, light stress, and UV-B radiation. However, in practice, these measures may not be effective enough to increase carotenoid content, and the dosage and energy consumption are suboptimal, potentially wasting resources and energy. Summary of the Invention

[0004] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides a method for promoting the accumulation of carotenoids in corn sprouts, which can increase the total amount of carotenoids in corn sprouts.

[0005] The invention also provides a corn sprout with high carotenoid accumulation.

[0006] According to one aspect of the present invention, a method for promoting carotenoid accumulation in corn sprouts is proposed. The method comprises the following steps: soaking corn seeds and then sowing them on a seed germination bed; spraying a 10-200 μM tryptamine solution on the corn seeds, and culturing them under light until the embryo stage to obtain corn sprouts.

[0007] In some embodiments of the present invention, the corn seeds are required to be plump, complete, mold-free, insect-free, and fresh.

[0008] In some embodiments of the present invention, the corn seeds further include a soaking and disinfection step before the seed soaking treatment.

[0009] In some embodiments of the present invention, the reagent used for the immersion disinfection includes sodium hypochlorite.

[0010] In some embodiments of the present invention, the volume concentration of the sodium hypochlorite is 5% to 10%.

[0011] In some embodiments of the present invention, the volume concentration of the sodium hypochlorite is 6% to 8%.

[0012] In some embodiments of the present invention, the immersion disinfection time is 5-15 minutes.

[0013] In some embodiments of the present invention, the immersion disinfection time is 8-12 minutes.

[0014] In some embodiments of the present invention, the soaking disinfection further includes a step of rinsing with deionized water.

[0015] In some embodiments of the present invention, the deionized water rinse frequency is 5 to 20 times.

[0016] In some embodiments of the present invention, the seed soaking treatment time is 8-15 hours.

[0017] In some embodiments of the present invention, the seed soaking treatment time is 11-13 hours.

[0018] In some embodiments of the present invention, the filter paper and absorbent cotton are laid on the seed germination bed from top to bottom.

[0019] In some embodiments of the present invention, the seed germination bed is a seed germination bed that has been soaked with a 10-200 μM tryptamine solution.

[0020] In some embodiments of the present invention, the concentration of the tryptamine solution is 10-200 μM.

[0021] In some embodiments of the present invention, the spraying amount of the tryptamine solution is 0.5 to 2 L / m 2 .

[0022] In some embodiments of the present invention, the amount of the tryptamine solution sprayed is 1 L / m 2 .

[0023] In some embodiments of the present invention, the illumination is performed using monochromatic light.

[0024] In some embodiments of the present invention, the wavelength of the monochromatic light is 300-600 nm.

[0025] In some embodiments of the present invention, the wavelength of the monochromatic light is 400-550 nm.

[0026] In some embodiments of the present invention, the intensity of the monochromatic light is 10-20 μmol·m -2 ·s -1 .

[0027] In some embodiments of the present invention, the culture temperature is 20-30°C.

[0028] In some embodiments of the present invention, the culture temperature is 25-30°C.

[0029] In some embodiments of the present invention, the culture time is 2-4 days.

[0030] According to a second aspect of the present invention, a corn sprout with high carotenoid accumulation is provided. The corn sprout is prepared by the above method.

[0031] According to some embodiments of the present invention, at least the following beneficial effects are achieved: the present method synergistically treats corn seeds with tryptamine and monochromatic light during germination. By controlling the tryptamine concentration and the wavelength and intensity of the monochromatic light, it effectively promotes carotenoid accumulation in corn sprouts, thereby cultivating corn sprouts with a high total carotenoid content. This invention provides theoretical support for the cultivation of high-value-added corn sprouts and has good economic benefits. DETAILED DESCRIPTION

[0032] The following will clearly and completely describe the concept and technical effects of the present invention in conjunction with the embodiments to fully understand the purpose, features and effects of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0033] If the specific conditions are not specified in the examples, the experiments were carried out under conventional conditions or those recommended by the manufacturer. All reagents or instruments used, if the manufacturer is not specified, are commercially available conventional products.

[0034] Example 1

[0035] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0036] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0037] (2) Sow corn seeds in a soil soaked with 10 μmol·L-1 The cells were cultured on cotton wool and filter paper containing tryptamine solution in the dark at a temperature of 28°C.

[0038] (3) Apply 1 L of 10 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were harvested after incubation with a tryptamine solution for 72 hours (i.e., the embryo stage). The total amount of carotenoids in the corn sprouts was determined.

[0039] Example 2

[0040] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0041] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0042] (2) Sow corn seeds in a soil soaked with 20 μmol·L -1 The cells were cultured on cotton wool and filter paper containing tryptamine solution in the dark at a temperature of 28°C.

[0043] (3) Apply 1 L of 20 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0044] Example 3

[0045] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0046] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0047] (2) Sow corn seeds on a soil soaked with 50 μmol·L -1 The cells were cultured on cotton wool and filter paper containing tryptamine solution in the dark at a temperature of 28°C.

[0048] (3) Apply 1 L of 50 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0049] Example 4

[0050] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0051] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0052] (2) Sow corn seeds on a soil soaked with 100 μmol·L -1 The cells were cultured on cotton wool and filter paper containing tryptamine solution in the dark at a temperature of 28°C.

[0053] (3) Apply 1 L of 100 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0054] Example 5

[0055] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0056] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0057] (2) Sow corn seeds in a soil soaked with 150 μmol·L -1 The cells were cultured on cotton wool and filter paper containing tryptamine solution in the dark at a temperature of 28°C.

[0058] (3) Apply 1L of 150μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0059] Example 6

[0060] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0061] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0062] (2) Sow corn seeds on a soil soaked with 200 μmol·L -1 The cells were cultured on cotton wool and filter paper containing tryptamine solution in the dark at a temperature of 28°C.

[0063] (3) Apply 1 L of 200 μmol·L per square meter of cultivation area every day -1The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0064] Example 7

[0065] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0066] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0067] (2) Corn seeds were sown on absorbent cotton and filter paper soaked in deionized water, and the monochromatic light wavelength was 550 nm and the light intensity was 10 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0068] (3) 1 L of deionized water was applied per square meter of cultivated area daily for 72 hours, and the corn sprouts were harvested. The total amount of carotenoids in the corn sprouts was measured.

[0069] Example 8

[0070] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0071] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0072] (2) Sow corn seeds in a soil soaked with 10 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 550 nm and a light intensity of 10 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0073] (3) Apply 1 L of 10 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0074] Example 9

[0075] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0076] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0077] (2) Sow corn seeds in a soil soaked with 20 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 550 nm and a light intensity of 10 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0078] (3) Apply 1 L of 20 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0079] Example 10

[0080] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0081] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0082] (2) Sow corn seeds on a soil soaked with 50 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 550 nm and a light intensity of 10 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0083] (3) Apply 1 L of 50 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0084] Example 11

[0085] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0086] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0087] (2) Sow corn seeds on a soil soaked with 100 μmol·L -1The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 550 nm and a light intensity of 10 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0088] (3) Apply 1 L of 100 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0089] Example 12

[0090] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0091] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0092] (2) Sow corn seeds in a soil soaked with 150 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 550 nm and a light intensity of 10 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0093] (3) Apply 1L of 150μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0094] Example 13

[0095] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0096] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0097] (2) Sow corn seeds on a soil soaked with 200 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 550 nm and a light intensity of 10 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0098] (3) Apply 1 L of 200 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0099] Example 14

[0100] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0101] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0102] (2) Corn seeds were sown on cotton wool and filter paper soaked in deionized water, and the light intensity was 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0103] (3) 1 L of deionized water was applied per square meter of cultivated area daily for 72 hours, and the corn sprouts were harvested. The total amount of carotenoids in the corn sprouts was measured.

[0104] Example 15

[0105] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0106] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0107] (2) Sow corn seeds in a soil soaked with 10 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 500 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0108] (3) Apply 1 L of 10 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0109] Example 16

[0110] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0111] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0112] (2) Sow corn seeds in a soil soaked with 20 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 500 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0113] (3) Apply 1 L of 20 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0114] Example 17

[0115] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0116] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0117] (2) Sow corn seeds on a soil soaked with 50 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 500 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0118] (3) Apply 1 L of 50 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0119] Example 18

[0120] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0121] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0122] (2) Sow corn seeds on a soil soaked with 100 μmol·L-1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 500 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0123] (3) Apply 1 L of 100 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0124] Example 19

[0125] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0126] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0127] (2) Sow corn seeds in a soil soaked with 150 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 500 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0128] (3) Apply 1L of 150μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0129] Example 20

[0130] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0131] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0132] (2) Sow corn seeds on a soil soaked with 200 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 500 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0133] (3) Apply 1 L of 200 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0134] Example 21

[0135] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0136] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0137] (2) Corn seeds were sown on cotton wool and filter paper soaked in deionized water, and the monochromatic light wavelength was 450 nm and the light intensity was 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0138] (3) 1 L of deionized water was applied per square meter of cultivated area daily for 72 hours, and the corn sprouts were harvested. The total amount of carotenoids in the corn sprouts was measured.

[0139] Example 22

[0140] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0141] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0142] (2) Sow corn seeds in a soil soaked with 10 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 450 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0143] (3) Apply 1 L of 10 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0144] Example 23

[0145] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0146] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0147] (2) Sow corn seeds in a soil soaked with 20 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 450 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0148] (3) Apply 1 L of 20 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0149] Example 24

[0150] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0151] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0152] (2) Sow corn seeds on a soil soaked with 50 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 450 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0153] (3) Apply 1 L of 50 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0154] Example 25

[0155] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0156] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0157] (2) Sow corn seeds on a soil soaked with 100 μmol·L-1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 450 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0158] (3) Apply 1 L of 100 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0159] Example 26

[0160] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0161] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0162] (2) Sow corn seeds in a soil soaked with 150 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 450 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0163] (3) Apply 1L of 150μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0164] Example 27

[0165] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0166] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0167] (2) Sow corn seeds on a soil soaked with 200 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 450 nm and a light intensity of 15 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0168] (3) Apply 1 L of 200 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0169] Example 28

[0170] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0171] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0172] (2) Corn seeds were sown on absorbent cotton and filter paper soaked in deionized water, and the light intensity was 20 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0173] (3) 1 L of deionized water was applied per square meter of cultivated area daily for 72 hours, and the corn sprouts were harvested. The total amount of carotenoids in the corn sprouts was measured.

[0174] Example 29

[0175] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0176] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0177] (2) Sow corn seeds in a soil soaked with 10 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 400 nm and a light intensity of 20 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0178] (3) Apply 1 L of 10 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0179] Example 30

[0180] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0181] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0182] (2) Sow corn seeds in a soil soaked with 20 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 400 nm and a light intensity of 20 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0183] (3) Apply 1 L of 20 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0184] Example 31

[0185] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0186] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0187] (2) Sow corn seeds on a soil soaked with 50 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 400 nm and a light intensity of 20 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0188] (3) Apply 1 L of 50 μmol·L per square meter of cultivation area every day. -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0189] Example 32

[0190] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0191] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0192] (2) Sow corn seeds on a soil soaked with 100 μmol·L-1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 400 nm and a light intensity of 20 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0193] (3) Apply 1 L of 100 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0194] Example 33

[0195] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0196] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0197] (2) Sow corn seeds in a soil soaked with 150 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 400 nm and a light intensity of 20 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0198] (3) Apply 1L of 150μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0199] Example 34

[0200] This embodiment prepares a corn sprout with a high carotenoid content. The specific preparation process is as follows:

[0201] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0202] (2) Sow corn seeds on a soil soaked with 200 μmol·L -1 The tryptamine solution was placed on the absorbent cotton and filter paper under the condition of monochromatic light with a wavelength of 400 nm and a light intensity of 20 μmol·m -2 ·s -1 The culture temperature was 28℃;

[0203] (3) Apply 1 L of 200 μmol·L per square meter of cultivation area every day -1 The corn sprouts were incubated with a tryptamine solution for 72 hours and then harvested. The total amount of carotenoids in the corn sprouts was determined.

[0204] Comparative Example 1

[0205] This comparative example prepared a corn sprout with a high carotenoid content, and the specific preparation process was as follows:

[0206] (1) Soak corn seeds in a 7% by volume sodium hypochlorite solution for 10 minutes, then rinse with deionized water 13 times, and soak the corn seeds in deionized water for 12 hours to allow the seeds to swell;

[0207] (2) Sow corn seeds on cotton wool soaked in deionized water and filter paper and culture in the dark at a temperature of 28°C;

[0208] (3) 1 L of deionized water was applied per square meter of cultivated area daily for 72 hours, and the corn sprouts were harvested. The total amount of carotenoids in the corn sprouts was measured.

[0209] Test example

[0210] This test example tested the carotenoid content in the corn sprout samples prepared in Examples 1-34 and Comparative Example 1.

[0211] Carotenoid Extraction: Corn sprouts (prepared in Examples 1-34 and Comparative Example 1, respectively) were ground with liquid nitrogen until powdered. 1 g of corn sprout powder was weighed and mixed with 2 mL of 95% (v / v) ethanol solution, 1 mL of sodium chloride solution (17.52 g / L), 4 mL of ethanol-dissolved pyrogallic acid (63.055 g / L), and 1 mL of ascorbic acid solution (176 mg / mL). 2 mL of potassium hydroxide solution (600 g / L) was then added. The mixture was saponified in a 75°C water bath for 45 min, with multiple shakes during the saponification period. The mixture was then cooled to room temperature in an ice-water bath. 0.75 mL of sodium chloride solution (175.2 g / L) was then added, and the mixture was extracted three times with 10 mL of a 1:9, v / v mixture of ethyl acetate and n-hexane. The upper organic phases were collected and dried using a nitrogen blowdown apparatus. The solution was reconstituted with 1 mL of methyl tert-butyl ether solution containing 0.1% 2,6-di-tert-butyl-p-cresol, filtered with a 0.22 μL organic phase filter membrane, and stored in the dark at -20°C until testing.

[0212] Carotenoid Assay: The extracted samples were assayed using high-performance liquid chromatography (HPLC). The detection wavelength for carotenoids was 450 nm. Common carotenoid components found in corn, such as violaxanthin, neoxanthin, antheraxanthin, lutein, zeaxanthin, α-cryptoxanthin, ε-carotene, α-carotene, and β-carotene, were used as standards to determine the content of the corresponding compounds in the samples. The results are shown in Table 1.

[0213] Table 1

[0214]

[0215]

[0216] From the experimental results in Table 1, the following conclusions can be drawn:

[0217] The FW in the table is fresh weight. The synergistic treatment of hormones and monochromatic light helps corn sprouts accumulate carotenoids, thereby significantly increasing the total amount of carotenoids in corn sprouts and enhancing the nutritional quality of corn sprouts.

[0218] Compared with the condition without hormone and monochromatic light treatment in comparative example 1, the appropriate increase of tryptamine concentration in examples 1 to 6 can effectively increase the total amount of carotenoids in corn sprouts. -1 The total amount of carotenoids in the corn sprouts treated with tryptamine (Example 2) was increased by 52.16% compared with the corn sprouts not treated with tryptamine (Comparative Example 1). -1 The total carotenoid content of corn sprouts obtained by treatment with tryptamine concentration was the highest. It can be seen that treatment with appropriate concentration of tryptamine is helpful to accumulate the total carotenoid content of corn sprouts.

[0219] Compared with the condition without hormone and monochromatic light treatment in Comparative Example 1, the application of monochromatic light with different wavelengths and light intensities in Examples 7, 14, 21, and 28 can effectively increase the total amount of carotenoids in corn sprouts. -2 ·s -1 The total amount of carotenoids in the corn sprouts treated with monochromatic light (Example 21) was increased by 1251.5% compared with the corn sprouts not treated with monochromatic light (Comparative Example 1). -2 ·s -1 The total amount of carotenoids in corn sprouts treated with monochromatic light was the highest, which showed that monochromatic light treatment was helpful in accumulating the carotenoid content in corn sprouts.

[0220] Compared with the condition without hormone and monochromatic light treatment in Comparative Example 1, the synergistic treatment of tryptamine and monochromatic light in Examples 8-13, 15-20, 22-27, 29-34 can effectively increase the total amount of carotenoids in corn sprouts. -1 Tryptamine concentration and wavelength 450nm, light intensity 15μmol·m -2 ·s -1 The total amount of carotenoids in the corn sprouts (Example 25) treated with monochromatic light was increased by 1462.4% compared with the corn sprouts (Comparative Example 1) that were not treated with hormones and monochromatic light. -1 Tryptamine concentration and wavelength 450nm, light intensity 15μmol·m -2 ·s -1 The total amount of carotenoids in corn sprouts obtained by the synergistic treatment of tryptamine and monochromatic light was the highest. It can be seen that the synergistic treatment of tryptamine and monochromatic light is conducive to further accumulation of the total amount of carotenoids in corn sprouts.

[0221] In summary, the cultivation method of corn sprouts subjected to synergistic treatment of hormones and monochromatic light provided by the present invention helps corn sprouts accumulate carotenoids. Both hormone treatment and monochromatic light treatment are beneficial to the increase of the total amount of carotenoids in corn sprouts, but the synergistic treatment of tryptamine and monochromatic light has a significant gain effect on increasing the total amount of carotenoids in corn sprouts, which can effectively improve the nutritional quality and economic value of corn sprouts.

[0222] While the embodiments of the present invention have been described in detail above, the present invention is not limited to the embodiments described above. Various modifications may be made within the scope of knowledge possessed by a person skilled in the art without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof may be combined with one another unless there is a conflict.

Claims

1. A method for promoting the accumulation of carotenoids in corn sprouts, characterized in that: The method comprises the following steps: soaking corn seeds and then sowing them on a seed germination bed; spraying a 10-200 μM tryptamine solution on the corn seeds, and culturing them under light until the embryo stage to obtain corn sprouts; Wherein, the spraying amount of the tryptamine solution is 0.5~2L / m 2 ; The illumination is performed using monochromatic light, the wavelength of the monochromatic light is 450-550 nm, and the illumination intensity of the monochromatic light is 10-20 μmol·m -2 ·s -1 .

2. The method according to claim 1, characterized in that The corn seeds also include a soaking and disinfection step before the seed soaking treatment.

3. The method according to claim 2, characterized in that The reagent used for the immersion disinfection includes sodium hypochlorite.

4. The method according to claim 3, characterized in that The volume concentration of the sodium hypochlorite is 5% to 10%.

5. The method according to claim 2, characterized in that The soaking and disinfection time is 5-15 minutes.

6. The method according to claim 1, wherein The seed soaking treatment lasts for 8-15 hours.

7. The method according to claim 1, characterized in that The filter paper and absorbent cotton are laid on the seed germination bed from top to bottom.

8. The method according to claim 1, characterized in that The seed germination bed is a seed germination bed that has been soaked with a 10-200 μM tryptamine solution.

9. The method according to claim 1, characterized in that The culture temperature is 20-30°C.

10. The method according to claim 1, characterized in that The culture time is 2-4 days.

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