Fresh-keeping liquid for cut roses and its preparation method

Abstract

The present invention provides a cut flower preservative solution and a preparation method thereof, belonging to the technical field of flower preservation. The rose cut flower preservative solution is prepared from the following raw materials: 1-10 μmol ramelteon, 10-30 g glucose, 5-10 g seaweed oligosaccharides, 80-120 mg aluminum sulfate, 40-60 μmol sodium alginate, 20-30 μmol carboxymethyl cellulose, and 1 L of acidic electrolyzed water. The rose cut flower preservative solution proposed in the present invention is mainly used for preserving fresh rose cut flowers. During the vase period, if the preservative solution is not replaced, the cut flowers can be vase-inserted for up to 16 days. The flowering index, flower stem wilting index, and wilting index of the cut flowers are all low, and the vase-insertion effect is far superior to the 7-8 days vase-insertion viewing period of the prior art Keli fresh vase-insertion solution.

Description

Technical Field

[0001] The invention belongs to the technical field of fresh-keeping of fresh flowers, and specifically discloses a fresh-keeping liquid for cut rose flowers and a preparation method thereof. Background Art

[0002] Currently, my country's annual sales of fresh cut flowers reach over 23 billion stems. At the Dounan Flower Market in Kunming, Yunnan alone, 1.2 to 1.5 million stems are traded daily, with a turnover exceeding 1 million yuan. This demonstrates the enormous potential for future growth in the fresh cut flower industry. However, the post-harvest loss rate for fresh cut flowers in my country exceeds 30%, and the loss rate during long-distance transportation is even greater than 40%, compared to only 2% in developed countries. Therefore, improving the quality of cut flowers and extending their viewing period are urgent challenges facing my country's fresh cut flower industry.

[0003] After fresh cut flowers are separated from their mother plants, their respiratory activity accelerates, their water absorption rate decreases, their fresh weight decreases, and they rapidly decline. Water transport and absorption within the flower are key factors influencing water loss and wilting. Factors affecting water absorption include blockage of the ducts at the base of the stem and in the xylem, bacterial growth and metabolic product coverage, blockage caused by latex and resinous substances secreted from the cut surface, and mechanical trauma caused by air entering the ducts during cutting.

[0004] Unlike edible flowers, which can be frozen to significantly extend their shelf life, ornamental cut flowers are primarily preserved with vase solution. This solution's primary purpose is to inhibit microbial growth, replenish nutrients, and suppress ethylene production. It also inhibits the activity of hydrolases and proteases within the cut flower, preventing physiological blockage of the flower stems and reducing transpiration. Currently, the most commonly used vase solution is the Kelixian brand imported from the Netherlands. For example, cut roses, without vase solution, only last for 3-4 days, while with Kelixian, they last for 7-8 days.

[0005] In recent years, researchers have found that melatonin has a certain preservation effect on cut flowers [Luo Tongtong et al. Effect of melatonin on the preservation effect of cut rose 'Carola' [J] Journal of Zhejiang A&F University, 2018, 35(5): 981-986]. However, the use of melatonin for preservation can only extend the preservation time to a maximum of 7 days. Therefore, the development of a new rose cut flower preservation solution that can extend the vase viewing period of cut flowers and improve the quality of cut flowers is of great significance for increasing the commercial value of cut flowers. Summary of the Invention

[0006] The invention provides a rose cut flower preservative liquid and a preparation method thereof, which can effectively solve the problem of a short vase inserting period when the existing vase inserting preservative liquid is applied to rose cut flowers.

[0007] The present invention provides a rose cut flower preservative liquid, which is prepared from the following raw materials:

[0008] 1-10 μmol ramelteon, 10-30 g glucose, 5-10 g seaweed oligosaccharides, 80-120 mg aluminum sulfate, 40-60 μmol sodium alginate, 20-30 μmol carboxymethyl cellulose, 1 L acidic electrolyzed water.

[0009] Further, it is prepared from the following raw materials,

[0010] 5-10 μmol ramelteon, 10-30 g glucose, 5-10 g seaweed oligosaccharides, 80-120 mg aluminum sulfate, 40-60 μmol sodium alginate, 20-30 μmol carboxymethyl cellulose, 1 L acidic electrolyzed water.

[0011] The present invention also provides a method for preparing any of the above-mentioned rose cut flower preservative liquids, comprising the following steps:

[0012] 1) Add glucose, seaweed oligosaccharide and carboxymethyl cellulose to acidic electrolyzed water and mix well;

[0013] 2) Add sodium alginate, mix well, and adjust the pH of the solution to 4.0-4.5;

[0014] 3) Add ramelteon and aluminum sulfate, mix well, and adjust the pH of the solution to 4.0-4.5 to obtain a rose cut flower preservative solution.

[0015] Furthermore, in step 2), neutral electrolyzed water is added to adjust the pH of the solution to 4.0-4.5.

[0016] Furthermore, in step 3), acidic electrolyzed water is added to adjust the pH of the solution to 4.0-4.5.

[0017] Furthermore, in step 3), ultrasound is used for mixing.

[0018] Furthermore, in step 3), the frequency of ultrasound is 40-50 kHz, and the power of ultrasound is 240-260 W.

[0019] Furthermore, the pH of the acidic electrolyzed water is 2.0-2.5.

[0020] Furthermore, the pH of the neutral electrolyzed water is 6.0-7.5.

[0021] The invention also provides the rose cut flower preservative liquid, and the rose cut flower preservative liquid prepared by the preparation method is used in rose cut flower preservation, wherein the mass ratio of the rose cut flower preservative liquid to water is 1:50-200.

[0022] The present invention has the following advantages:

[0023] The rose cut flower preservative liquid proposed by the present invention is composed of ramelteon, glucose, seaweed oligosaccharides, aluminum sulfate, sodium alginate, carboxymethyl cellulose, and acidic electrolyzed water in appropriate proportions. Ramelteon's high lipophilicity makes it easily absorbed by plant tissues, and it has efficient antioxidant properties, which can scavenge reactive oxygen free radicals, induce protein oxidative damage repair enzymes, regulate energy metabolism, and inhibit ethylene synthesis. The synergistic effect of carboxymethyl cellulose and sodium alginate can effectively remove latex and resin secreted from the cut surface of fresh flowers, improving adsorption efficiency. Aluminum sulfate can also be used for sterilization to prevent blockage of cut flower ducts. Ultimately, water-soluble components such as glucose, seaweed oligosaccharides, and water molecules are more easily absorbed into plant cells, significantly improving the absorption of nutrients and water. Experimental results show that the rose cut flower preservative liquid proposed in the present invention can maintain a vase-insertion period of up to 16 days if the preservative liquid is not replaced. In terms of sensory photos, flowering index, flower stem wilting index, wilting index, weight loss rate, rot rate, and maximum flower diameter of the cut flowers, the preservative effect is superior to the prior art Kelixian vase-insertion liquid, whose vase-insertion viewing period is only 7-8 days. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The accompanying drawings, which constitute part of the present invention, are provided to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are provided to explain the present invention and do not constitute an undue limitation of the present invention. In the accompanying drawings:

[0025] Figure 1 Schematic diagram of sensory photos of fresh cut flowers treated differently in the embodiment of the present invention and the comparative example;

[0026] Figure 2 This is a graph showing weight loss rates of fresh cut flowers treated differently in the embodiments of the present invention and the comparative example;

[0027] Figure 3 This is a diagram showing the decay rate of fresh cut flowers treated differently in the embodiments of the present invention and the comparative example;

[0028] Figure 4 The figures are effect diagrams of the maximum flower diameters of fresh cut flowers treated differently in the embodiments of the present invention and the comparative examples. DETAILED DESCRIPTION

[0029] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0030] The inventor has found through research that melatonin, ramelteon and agomelatine are all melatonin receptor agonists, which are mainly used in the preparation of medicines for human consumption, and play a role by exciting melatonin receptors (MT1 and MT2 receptors). However, the half-life of these three receptor agonists in vivo is different, and the half-life of melatonin is 40 minutes, ramelteon is 1-2.6 hours, and agomelatine is 1-2 hours. The affinity of ramelteon and agomelatine to melatonin receptors is greater than melatonin. It can be seen from this that for the medicine of the same dose, the duration of ramelteon is the longest, and the drug effect is played for the longest time. And agomelatine can cause the increase of organism transaminase, easily causes liver failure, and also has an impact on fresh-keeping of fresh flowers. Therefore, the inventor first proposes that ramelteon be used to prepare Chinese rose cut flower fresh-keeping liquid.

[0031] One embodiment of the present invention provides a rose cut flower preservative liquid, which is prepared from the following raw materials:

[0032] 1-10 μmol ramelteon, 10-30 g glucose, 5-10 g seaweed oligosaccharides, 80-120 mg aluminum sulfate, 40-60 μmol sodium alginate, 20-30 μmol carboxymethyl cellulose, 1 L acidic electrolyzed water.

[0033] In a preferred embodiment of the present invention, the rose cut flower preservative liquid is prepared from the following raw materials:

[0034] 5-10 μmol ramelteon, 10-30 g glucose, 5-10 g seaweed oligosaccharides, 80-120 mg aluminum sulfate, 40-60 μmol sodium alginate, 20-30 μmol carboxymethyl cellulose, 1 L acidic electrolyzed water.

[0035] In the rose cut flower preservative solution proposed in the embodiment of the present invention, the high lipophilicity of ramelteon makes it easily absorbed by plant tissues, and it has efficient antioxidant properties, which can remove reactive oxygen free radicals, induce protein oxidative damage repair enzymes, regulate energy metabolism and inhibit ethylene synthesis. The synergistic effect of carboxymethyl cellulose and sodium alginate can effectively remove the emulsion and resin secreted by the cut surface of the fresh flower, improve adsorption efficiency, and aluminum sulfate can also be used for sterilization to avoid blockage of the cut flower conduit, ultimately making the water-soluble components glucose, seaweed oligosaccharides and water molecules more easily enter plant cells, significantly improving the absorption of nutrients and water. It can be seen that the comprehensive effect between the various components enables the cut flowers to obtain enough water and nutrients from the preservative solution, thereby effectively extending the shelf life. Experimental results show that the rose cut flower preservative liquid proposed in the present invention can maintain a vase-insertion period of up to 16 days if the preservative liquid is not replaced. In terms of sensory photos, flowering index, flower stem wilting index, wilting index, weight loss rate, rot rate, and maximum flower diameter of the cut flowers, the preservative effect is superior to the prior art Kelixian vase-insertion liquid, whose vase-insertion viewing period is only 7-8 days.

[0036] Specifically, in the embodiment of the present invention,

[0037] Ramelteon, a melatonin receptor agonist, is used in fresh rose cut flower preservatives to demonstrate multiple benefits. First, ramelteon is highly lipophilic and easily absorbed and stored by plant tissues. It can also scavenge free radicals, maintain cell membrane integrity, regulate fruit maturation and aging, improve stress resistance and disease resistance, and slow the post-harvest aging process of cut flowers. Furthermore, ramelteon participates in post-harvest polyphenol metabolism, carbon metabolism, ethylene synthesis, and its signal transduction pathways, promoting the expression of cell wall-modifying proteins polygalacturonase, pectinesterase, and expansin, maintaining the firmness of the flower stems. Furthermore, ramelteon can inhibit ethylene synthesis, induce protein oxidative damage repair enzymes, and improve energy metabolism, thereby slowing the aging of cut flowers. Finally, ramelteon can enhance antioxidant capacity, promote the synthesis of proline and polyamines, and improve the cold resistance of cut flowers. It can also enhance the disease resistance of fresh flowers by promoting the accumulation of salicylic acid and jasmonic acid, activating the activity of defense enzymes.

[0038] Carboxymethyl cellulose has good mechanical properties and biodegradability. When dispersed in a solvent, it can form an entangled three-dimensional network structure and produce physical and chemical effects (van der Waals force, hydrogen bond, electrostatic force, hydrophobic force, etc.). It is mainly used to adsorb the latex and resin secreted by the cut surface of fresh flowers.

[0039] Sodium alginate can reduce the surface tension of water, increase the contact area between the solution and the cross section of cut flowers, and make it easier for water-soluble nutrients to enter plant cells through the cross section.

[0040] When carboxymethyl cellulose and sodium alginate are added at the same time, due to their compatibility at low concentrations, sodium alginate-carboxymethyl cellulose gel micropolymers can be formed, which improves the adsorption efficiency of the emulsion and resin in the bottle insertion liquid and prevents clogging.

[0041] Glucose mainly provides nutrition, and its solubility and absorption properties by plants are much higher than sucrose.

[0042] Seaweed oligosaccharides are small molecular fragments formed by enzymatic degradation of seaweed polysaccharides (alginic acid). Seaweed oligosaccharides have good water solubility, can promote the thickening of vascular bundles, and help increase the absorption rate of nutrient solution. Seaweed oligosaccharides can also induce resistance to stress and disease.

[0043] Aluminum sulfate is mainly used for sterilization to prevent microorganisms and metabolic products from clogging the catheter.

[0044] One embodiment of the present invention further provides a method for preparing a rose cut flower preservative liquid, comprising the following steps:

[0045] 1) Add glucose, seaweed oligosaccharide and carboxymethyl cellulose to acidic electrolyzed water and mix well;

[0046] 2) Add sodium alginate, mix well, and adjust the pH of the solution to 4.0-4.5;

[0047] 3) Add ramelteon and aluminum sulfate, mix well, and adjust the pH of the solution to 4.0-4.5 to obtain a rose cut flower preservative solution.

[0048] Furthermore, in step 2), neutral electrolyzed water is added to adjust the pH of the solution to 4.0-4.5.

[0049] Furthermore, in step 3), acidic electrolyzed water is added to adjust the pH of the solution to 4.0-4.5. It should be noted that the amount of acidic electrolyzed water added here is not large and its contribution to the concentration can be ignored.

[0050] Furthermore, in step 3), ultrasound is used for mixing. Specifically, the frequency of ultrasound is 40-50 kHz, and the power of ultrasound is 240-260 W.

[0051] Furthermore, the pH of the acidic electrolyzed water is 2.0-2.5. Preferably, the acidic electrolyzed water is produced by electrolysis in an electrolytic cell with a diaphragm for 1-1.5 hours.

[0052] Furthermore, the pH of the neutral electrolyzed water is 6.0-7.5. Preferably, the neutral electrolyzed water is produced by electrolysis in an electrolytic cell without a diaphragm for 30 min-40 min. Specifically, the pH of the neutral electrolyzed water can be 6.0, 6.5, 7, 7.5, etc.

[0053] One embodiment of the present invention further provides the above-mentioned rose cut flower preservative liquid, and the use of the rose cut flower preservative liquid prepared by the above-mentioned preparation method in the preservation of rose cut flowers, wherein the rose cut flower preservative liquid is mixed with water and then used, wherein the mass ratio of the rose cut flower preservative liquid to water is 1:50~200.

[0054] Specifically, the method for using the rose cut flower preservative liquid comprises the following steps:

[0055] After the cut flowers are harvested from the production area, they are immediately placed in a mixed solution of the rose cut flower preservative solution and water, and the flowers are grown at the production area for 1-2 hours before being transported to a packaging and subpackaging processing center, where the cut flowers are pruned, graded, and packaged before being marketed. The mass ratio of the rose cut flower preservative solution to water is 1:50 to 200. Preferably, the mass ratio of the rose cut flower preservative solution to water is 1:100.

[0056] The rose cut flower preservative liquid obtained in the embodiment of the present invention can be stored at room temperature for 24 months and has a long shelf life.

[0057] Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

[0058] Example 1 Preparation method of rose cut flower preservative liquid

[0059] Rose cut flower preservation solution was prepared from the following raw materials: 30 g glucose, 6 g seaweed oligosaccharide, 100 mg aluminum sulfate, 40 μmol sodium alginate, 5 μmol ramelteon, 20 μmol carboxymethyl cellulose, and 1 L acidic electrolyzed water;

[0060] The preparation method of rose cut flower preservative liquid comprises the following steps:

[0061] 1) preparing acidic electrolyzed water;

[0062] 2) Adding glucose, seaweed oligosaccharide and carboxymethyl cellulose to the acidic electrolyzed water and stirring;

[0063] 3) Continue adding sodium alginate, stirring, and adding neutral electrolyzed water to adjust the pH of the solution to 4.2;

[0064] 4) adding ramelteon and aluminum sulfate, and mixing the solution uniformly by ultrasonic vibration (ultrasonic frequency of 45 kHz, ultrasonic power of 250 W), and adding acidic electrolyzed water to adjust the pH of the solution to 4.2;

[0065] Acidic electrolyzed water and neutral electrolyzed water are used for regulation;

[0066] Acidic electrolyzed water has a pH of 2.0 and is produced by electrolysis in a diaphragm electrolytic cell for 1-1.5 hours;

[0067] The pH of neutral electrolyzed water is 6.0, and it is produced by electrolysis in an electrolytic cell without a diaphragm for 30 min-40 min.

[0068] Example 2

[0069] Same as Example 1, except that the amount of ramelteon is 1 μmol.

[0070] Example 3

[0071] Same as Example 1, except that the amount of ramelteon is 10 μmol.

[0072] Comparative Example 1

[0073] Same as Example 1, except that ramelteon is not added.

[0074] Comparative Example 2

[0075] Same as Example 1, except that 5 μmol melatonin is used instead of 5 μmol ramelteon.

[0076] Comparative Example 3

[0077] Same as Example 1, except that 20 μmol melatonin is used instead of 5 μmol ramelteon.

[0078] Comparative Example 4

[0079] Same as Example 1, except that 5 μmol agomelatine was used instead of 5 μmol ramelteon.

[0080] Comparative Example 5

[0081] Same as Example 1, except that no carboxymethyl cellulose was added.

[0082] Comparative Example 6

[0083] Same as Example 1, except that sodium alginate is not added.

[0084] Comparative Example 7

[0085] Same as Example 1, except that 30 g of sucrose is used instead of 30 g of glucose.

[0086] Comparative Example 8

[0087] Same as Example 1, except that 1 L of distilled water is used instead of 1 L of acidic electrolyzed water.

[0088] Comparative Example 9

[0089] Commercially available Coleslaw was used as a preservative.

[0090] Test Example 1 Determination of the preservation effect of preservative solution

[0091] 1. Measurement indicators and methods

[0092] (1) Average flower diameter

[0093] Starting from the day of vase insertion, the diameter of the flowers of each treatment was measured with a vernier caliper every 4 days, and the flower diameter was expressed as the maximum flower diameter.

[0094] (2) Change rate of fresh weight of cut flowers

[0095] From the time the cut flowers are placed in the vase, the fresh weight of the cut flowers is measured every 4 days using an analytical balance. The calculation formula is:

[0096] Fresh weight change rate = (fresh weight on day n - initial fresh weight) / initial fresh weight × 100%.

[0097] Using a weighing method, the weight of rose branches was measured using an electronic balance at a fixed time every two days from the start of vase placement until the end of the vase life. The fresh weight at the start of the treatment was set as 100%, and the rate of change in fresh weight during the vase period was calculated.

[0098] (3) Water balance value

[0099] Starting from the day the flower branch is placed in the vase, at a certain time every 4 days, use the weighing method and use a tray balance to weigh the flower branch + solution + bottle. The difference between two consecutive weighing values is the water loss. Then weigh the solution + bottle. The difference between two consecutive weighing values of the solution + bottle is the water absorption. The water balance value = water absorption - water loss.

[0100] (4) Sensory photos

[0101] Photographs were taken with a SLR camera.

[0102] (5) Cut flower vase life

[0103] After the cut roses are harvested and placed in a vase, their ornamental quality is observed and recorded every 4 days. The classification of ornamental quality refers to GBT18247.1-2000 Major Flower Product Grades Part 1: Cut Rose Standards for Fresh Cut Flowers. Severe wilting of flowers, dry and wrinkled petals, darkening of flower color, dry and bent stems, etc. are used as the criteria for judging whether the cut flowers have lost their ornamental value. When the cut flowers lose their ornamental value, their vase life ends.

[0104] (6) Flowering index

[0105] Flowering index 1: The calyx is slightly loose, suitable for long-distance transportation;

[0106] Flowering index 2: The calyx extends out of the sepals and can be used for both long-distance and short-distance transportation;

[0107] Flowering index 3: The outer petals begin to loosen, suitable for short-distance transportation or nearby sales;

[0108] Flowering index 4: The inner petals begin to loosen and must be sold nearby.

[0109] The calculation formula for flowering index is:

[0110] Flowering index = ∑(flowering level × flowers of that level) / (highest flowering level × total flowers) × 100%

[0111] (7) Decay rate

[0112] According to the size of the flower rot area, it is divided into 4 levels:

[0113] Level 0, no decay;

[0114] Level 1, the rotten area is less than 10% of the flower area;

[0115] Level 2: The rotten area accounts for 10% to 30% of the flower area;

[0116] Level 3, the rotten area is greater than 30% of the flower area;

[0117] The formula for calculating the decay rate is:

[0118] Decay rate = ∑(decay level × flowers of that level) / (highest decay level × total flowers) × 100%

[0119] (8) Flower stem wilting index

[0120] Observe the shape of flower stems and flower heads:

[0121] Level 1: No bending, in an upright state;

[0122] Level 2: Some buds are bent, which is easy to bend;

[0123] Level 3: The flower buds are bent and some of the open flowers are bent, which is severe bending.

[0124] The calculation formula of flower stem wilting index is:

[0125] Wilting index = ∑(withering level × flowers of that level) / (highest withering level × total flowers) × 100%

[0126] 2. Preservation effect and analysis

[0127] Choose the cut rose variety Corolla, and select strong branches with basically uniform thickness and no diseases or insect pests. Keep only two compound leaves before vase placement, and trim the flower stems at a 45° angle underwater to a total length of 40 cm.

[0128] 150 mL of distilled water was placed in glass bottles, and the preservative solutions prepared in Examples 1-3 and Comparative Examples 1-9 were added, with a mass ratio of preservative solution to distilled water of 1:100. Pruned flower branches were placed in the glass bottles, which were stored at 24°C in the dark. Measurements were taken every four days for 16 days. The fresh-keeping effects of fresh flowers were measured using the above-mentioned indicators and methods. The specific results are shown below.

[0129] (1) Sensory photos of flowers with different treatments

[0130] Depend on Figure 1As shown, the roses in the comparative example 1 group had already wilted after 16 days of vase placement; at 12 days, most of the flowers in the groups failed to bloom normally, while the flowers in the treatments of Examples 1-3 could maintain their vase placement period for up to 16 days, indicating that the treatments in Examples 1-3 extended the vase placement period of the flowers by at least 4-8 days. Furthermore, a relatively low concentration of ramelteon can achieve a good preservation effect, saving costs for practical applications. Furthermore, in the later stages of vase placement, the preservation effect of cut flowers treated with ramelteon was significantly better than that of agomelatine.

[0131] (2) Effects of different treatments on the weight loss rate of fresh flowers

[0132] Depend on Figure 2 It can be seen that the weight loss rate of rose cut flowers increases with the increase of vase insertion time. The weight loss rate of the groups treated with Examples 1 to 3 is lower than that of the control group, indicating that the treatments with Examples 1 to 3 can delay the aging of fresh flowers.

[0133] (3) Effects of different treatments on flower decay rates

[0134] The rot rate of rose cut flowers increases with the increase of vase time. Figure 3 It can be seen that the decay rates of the groups in Examples 1 to 3 are lower than those of the other treatment groups, indicating that the treatment with the preservative solution proposed in the embodiments of the present invention can slow down the growth rate of the decay rate of roses and extend the vase life.

[0135] (4) Effects of different treatments on maximum flower diameter

[0136] Depend on Figure 4 As can be seen, the maximum flower diameter shows a trend of first increasing and then decreasing during the vase period. This phenomenon occurs because the flowers open first during the vase period, increasing in diameter; later in the vase period, the flowers begin to wilt and drop petals, decreasing in diameter. The treatment groups of Examples 1-3 were higher than the other treatment groups and maintained a relatively high diameter even in the later stages of vase period, indicating that the treatment groups of Examples 1-3 were able to maintain a larger maximum flower diameter, significantly outperforming the control group.

[0137] The experimental results show that the cut roses in the treatment groups of Examples 1 to 3 were well preserved and maintained their high ornamental value after 16 days in vase. However, in the control group 1, which was not treated with ramelteon, the petals had become loose and bent after 8 days in vase.

[0138] (5) Effects of different treatments on flower flowering index, flower stem wilting index, and wilting index

[0139] As shown in Table 1, the flowering index, stem wilting index, and wilting index of each treatment group showed an upward trend. These three indicators reflect the state of flower opening, the state of the stem, and the overall appearance of the flower, respectively.

[0140] The flowering index of Examples 1 to 3 during the vase period was lower than that of the treatment group, indicating that the treatment of the treatment group can slow down the physiological activities of the flowers, reduce the speed of flower blooming and aging, and achieve the effect of extending the vase period of the flowers.

[0141] Table 1 Effects of different treatments on flower flowering index, flower stem wilting index and wilting index

[0142]

[0143] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A rose cut flower preservative liquid, characterized in that: Prepared from the following raw materials, 1-10 μmol ramelteon, 10-30 g glucose, 5-10 g seaweed oligosaccharides, 80-120 mg aluminum sulfate, 40-60 μmol sodium alginate, 20-30 μmol carboxymethyl cellulose, 1 L acidic electrolyzed water.

2. The Chinese rose cut flower preservative according to claim 1, wherein Prepared from the following raw materials, 5-10 μmol ramelteon, 10-30 g glucose, 5-10 g seaweed oligosaccharides, 80-120 mg aluminum sulfate, 40-60 μmol sodium alginate, 20-30 μmol carboxymethyl cellulose, 1 L acidic electrolyzed water.

3. The method for preparing the rose cut flower preservative solution according to claim 1 or 2, wherein: The steps include: 1) Add glucose, seaweed oligosaccharide and carboxymethyl cellulose to acidic electrolyzed water and mix well; 2) Add sodium alginate, mix well, and adjust the pH of the solution to 4.0-4.5; 3) Add ramelteon and aluminum sulfate, mix well, and adjust the pH of the solution to 4.0-4.5 to obtain a rose cut flower preservative solution.

4. The preparation method according to claim 3, characterized in that In step 2), neutral electrolyzed water is added to adjust the pH of the solution to 4.0-4.

5.

5. The preparation method according to claim 3, characterized in that In step 3), acidic electrolyzed water is added to adjust the pH of the solution to 4.0-4.

5.

6. The preparation method according to claim 3, characterized in that In step 3), ultrasound is used for mixing.

7. The preparation method according to claim 6, characterized in that In step 3), the frequency of the ultrasound is 40-50 kHz, and the power of the ultrasound is 240-260 W.

8. The preparation method according to claim 3, characterized in that The pH of acidic electrolyzed water is 2.0-2.

5.

9. The preparation method according to claim 4, characterized in that The pH of neutral electrolyzed water is 6.0-6.

5.

10. Use of the rose cut flower preservative liquid according to claim 1 or 2 or the rose cut flower preservative liquid prepared by the preparation method according to any one of claims 3 to 9 in preserving rose cut flowers, characterized in that: The rose cut flower preservative liquid is mixed with water and then used, wherein the mass ratio of the rose cut flower preservative liquid to water is 1:50-200.

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