A short-cut nitrification rapid start-up process for high ammonia-nitrogen wastewater

Abstract

The present invention discloses a rapid start-up process for shortcut nitrification of wastewater with high ammonia nitrogen. First, according to the characteristics of shortcut nitrification of wastewater with high ammonia nitrogen, the pH, temperature, ammonia nitrogen concentration and dissolved oxygen concentration are adjusted before water inlet to make the inlet load meet the requirements of microbial degradation. Then, strain NZ-AY1 is added, and the rapid start-up process for shortcut nitrification is carried out under the conditions of high ammonia nitrogen concentration, 30-33 °C, and pH 7.0-8.5. The strain is classified and named as Geobacillus inquilinus ([[]] Parageobacillus toebii ) NZ-AY1, which has been deposited in the China General Microbiological Culture Collection Center. In this process, the ammonia nitrogen removal efficiency can reach over 95%, the ammonia oxidation efficiency can reach 87% to 95%, and the ability to resist various shock loads is strong, making it more stable in the treatment system. At the same time, the rapid expansion culture process of the strain is also disclosed, which has the advantages of simple operation, short production cycle and low production cost, has practical application value, and broad market prospects.

Description

Technical Field

[0001] The present invention belongs to the field of biological wastewater treatment, and particularly relates to a process for rapidly starting short-cut nitrification of wastewater with relatively high ammonia nitrogen. Background Art

[0002] The excessive accumulation of nitrogen in water bodies causes eutrophication of water bodies, seriously endangering the safety of the ecosystem. Generally, biological methods are used for wastewater denitrification. The basic principle of short-cut nitrification is to control the nitrification process in traditional biological denitrification to stay in the nitrite stage, and then nitrite is used as an electron acceptor for the short-cut nitrification reaction. In 1997, Delft University of Technology in the Netherlands successfully developed a new denitrification process on this principle: the SHARON (Single reactor for Highactivity Ammonia Removal Over Nitrite) process. This process mainly utilizes the differences in the physiological characteristics of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria, and controls the nitrification reaction process by inhibiting the growth of nitrite-oxidizing bacteria, that is, controlling the reaction in the stage of large accumulation of nitrite, and then directly carrying out short-cut nitrification. Compared with the traditional nitrification-denitrification process, the short-cut nitrification-denitrification process saves about 25% of the aeration volume, reduces the carbon dioxide emissions by 20%, reduces the sludge production by 30%, and is particularly advantageous in treating wastewater with high ammonia nitrogen or low C / N ratio. Scholars at home and abroad have carried out a large number of studies on this process. A key step is to control the reaction in the stage of stable accumulation of nitrite. Scholars at home and abroad have selectively inhibited nitrite-oxidizing bacteria by studying factors such as different pH, temperature, dissolved oxygen, free ammonia, free hydroxylamine, harmful substances, hydraulic load, and sludge retention time, screened out ammonia-oxidizing bacteria, and further used the short-cut nitrification-denitrification process to treat high ammonia nitrogen wastewater such as aquaculture wastewater and landfill leachate.

[0003] Although there are many factors that can lead to the accumulation of nitrite in the nitrification process, there is currently no sufficient theoretical explanation for this phenomenon, and the test results are also different (such as the inhibition concentration levels of free ammonia and dissolved oxygen), and at the same time, the existing technology has problems such as long culture time of ammonia-oxidizing bacteria and complex starting control conditions for short-cut nitrification. Further exploration is needed to control and maintain the accumulation of nitrite. The best way to solve this problem is to ferment a highly efficient ammonia-oxidizing bacterium and develop a highly efficient and stable short-cut nitrification biological treatment process. Summary of the Invention

[0004] To address the deficiencies of the prior art, the present invention provides a process for the rapid start-up of shortcut nitrification of high-concentration ammonia nitrogen wastewater and successfully ferments an ammonia-oxidizing bacterium. This strain can initiate the shortcut nitrification of high-concentration ammonia nitrogen wastewater and effectively cope with various phenomena of efficiency decline during the shortcut nitrification of high-concentration ammonia nitrogen wastewater. It has the characteristics of rapid reaction, long-term stability, cost savings, and simple operation, and solves the problems in the process of rapid start-up of the shortcut nitrification process for high-ammonia nitrogen wastewater.

[0005] The technical solution adopted by the present invention is as follows:

[0006] A process for the rapid start-up of shortcut nitrification of high-concentration ammonia nitrogen wastewater, including: adjusting the pH, temperature, dissolved oxygen, and free ammonia of the wastewater. First, adjust the pH, temperature, ammonia nitrogen concentration, and dissolved oxygen concentration before water inlet according to the characteristics of ammonia-oxidizing bacteria to make the inlet load meet the requirements of microbial degradation. Secondly, use the ammonia-oxidizing bacterium NZ-AY1 screened in the laboratory to carry out the rapid start-up process of shortcut nitrification under the conditions of temperature controlled at 30 - 33 °C, pH controlled at 7.0 - 8.5, and dissolved oxygen controlled at 4 - 6 mg / L.

[0007] The ammonia nitrogen concentration in the high-concentration ammonia nitrogen wastewater is 300 mg / L - 500 mg / L.

[0008] The dosage of ammonia-oxidizing bacterium NZ-AY1 is 2% - 10%.

[0009] The ammonia-oxidizing bacterium is classified and named as Geobacillus subterraneus subsp. in-situ composting ( Parageobacillus toebii ) NZ-AY1, which was deposited at the China General Microbiological Culture Collection Center on December 25, 2022, with the deposit number: CGMCC No. 26235, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0010] The application of the ammonia-oxidizing bacterium in the rapid start-up process of shortcut nitrification of high-concentration ammonia nitrogen wastewater:

[0011] After culturing the strain of Geobacillus subterraneus subsp. in-situ composting ( Parageobacillus toebii ) NZ-AY1, add it to the high-concentration ammonia nitrogen wastewater at 30 - 33 °C for the rapid start-up process of shortcut nitrification.

[0012] The culturing of the strain of Geobacillus subterraneus subsp. in-situ composting ( Parageobacillus toebii ) NZ-AY1 includes the steps of activation, transfer, and amplification culture, which are specifically as follows:

[0013] 1) Activation: Pick a single colony of ammonia-oxidizing bacterium from the solid plate and transfer it to the ammonia-oxidizing liquid medium. The shaking speed of the shaker is 130 - 160 rpm, and culture it in the shaker at 30 - 33 °C until the logarithmic phase;

[0014] 2) Transfer: Transfer the ammonia-oxidizing bacteria solution in the logarithmic phase to a seed tank for cultivation. Control the temperature of the seed tank to be maintained at 30 - 33 °C, the rotation speed to be kept at 220 - 250 rpm, the dissolved oxygen DO to be controlled at 4 - 6 mg / L, and cultivate for 5d - 10d;

[0015] 3) Amplification culture: Transfer the bacterial solution cultured in the seed tank to a fermentation tank for large-scale culture according to an inoculation amount of 2% - 5%. The components of the fermentation tank medium are the same as those of the seed tank, and the physical and chemical parameter indicators are: temperature 30 - 33 °C, rotation speed 230 - 260 rpm, dissolved oxygen 4 - 6 mg / L, and fermentation time 3 - 5d.

[0016] The components of the ammonia-oxidizing liquid medium: (NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 2 O 0.1 g, trace element solution 1 mL / L (zinc chloride 80 mg, anhydrous copper sulfate 20 mg, boric acid 20 mg, ferrous sulfate heptahydrate 100 mg), water 1000 mL, pH 7.8 - 8.5.

[0017] The medium components of the seed tank are: (NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 2 O 0.1 g, FeSO 4 ·7H 2 O 0.001 g, agar 20 g, trace element solution 1 mL / L, water 1000 mL, pH 7.8 - 8.5.

[0018] After cultivation, the effective viable count of the bacterial solution can reach 10 9 CFU / ml or more. After the fermentation culture solution is taken out of the tank and packaged, a high-efficiency ammonia-oxidizing bacterial agent can be obtained.

[0019] The application of the ammonia-oxidizing bacteria in the treatment of high-ammonia-nitrogen wastewater. First, according to the characteristics of the high-ammonia-nitrogen wastewater, the pH, pollutant concentration, and dissolved oxygen concentration are adjusted before water inlet to make the inlet load meet the requirements of microbial degradation (ammonia-nitrogen concentration is 300 mg / L - 500 mg / L, pH value is 7.0 - 8.5, dissolved oxygen is 4 - 6 mg / L). Secondly, the ammonia-oxidizing bacterial strain NZ-AY1 screened in the laboratory is added, and the shortcut nitrification process is carried out under the condition that the temperature is controlled at 30 - 33 °C.

[0020] The dosage of the ammonia-oxidizing bacteria NZ-AY1 is 1% - 10%.

[0021] The highly efficient ammonia-oxidizing bacterial species used in the microbial denitrification process ( Parageobacillus toebii ) NZ-AY1. In this laboratory, it was screened from the sediment of a certain river in Taizhou under normal temperature conditions using high-ammonia-nitrogen pollutants and preserved in the General Microbiology Center of the China Center for Type Culture Collection. This microorganism is cultured by expanding with a common medium, and the inoculation concentration is 5%. The residence time in the microbial shortcut nitrification process is 48 h - 72 h.

[0022] The strain of Bacillus subtilis ( Parageobacillus toebii ) NZ-AY1 of the present invention for in-situ composting was screened from the sediment of a certain river in Taizhou. The specific screening method is as follows:

[0023] Use: (NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 2 O 0.1 g, FeSO 4 ·7H 2 O 0.001 g, agar 20 g, dissolved in 1000 mL of distilled water. After being sterilized by high-temperature and high-pressure steam (120 °C, 20 min), it is used as an enrichment medium. Add 10 mL of river sediment taken from a certain river in Taizhou. After 7 days, extract 1 mL and put it into the newly prepared enrichment medium. Repeat this for 2 cycles. Inoculate the initially screened colonies into the ammonia-oxidizing medium ((NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 20.2 g of O, CaCl 2 ·2H 2 O, 0.1 g, trace element solution 1 mL / L (80 mg of zinc chloride, 20 mg of anhydrous copper sulfate, 20 mg of boric acid, 100 mg of ferrous sulfate heptahydrate), 1000 mL of water, pH 7.8 - 8.5)), cultured, and the NH 4 + -N, NO 3 - -N, NO 2 - -N content was measured every 24 h. According to the removal effect of the NH 4 + -N, NO 3 - -N, NO 2 - -N index, 1 strain with the performance of enriching higher NO 2 - -N was obtained through rescreening and named NZ-AY1.

[0024] The strain is elliptical, milky yellow, has a capsule, no spore, Gram-positive, heterotrophic, strictly aerobic, and positive for ammonia nitrogen nitrification test.

[0025] The culture conditions of the strain are: Take 1 mL of the bacterial solution and evenly coat it on the aerobic ammonia oxidation medium ((NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O, 0.2 g, CaCl 2 ·2H 2 O, 0.1 g, trace element solution 1 mL / L (80 mg of zinc chloride, 20 mg of anhydrous copper sulfate, 20 mg of boric acid, 100 mg of ferrous sulfate heptahydrate), 1000 mL of water, pH 7.8 - 8.5)), and the medium was placed in a constant temperature incubator for culture. The culture temperature was 30 - 33 °C, and the culture time was 7 d.

[0026] Application of the strain in the short-cut nitrification rapid start-up process of high ammonia nitrogen wastewater.

[0027] The strain can grow in wastewater at a temperature of 30 - 33 °C, and the doubling period is 48 h.

[0028] The ammonia-oxidizing bacterium NZ-AY1 screened in the present invention can grow in high-ammonia-nitrogen wastewater. Under the control conditions of a temperature of 30-33 °C and a pH of 7.0-8.5, it can achieve a rapid start of short-cut nitrification in high-ammonia-nitrogen wastewater. At the same time, the strain does not require an external carbon source and has a high degradation rate. Compared with other ammonia-oxidizing bacteria, it has the advantages of high ammonia oxidation efficiency, long-term stability, low cost, low energy consumption, few bacteria in the effluent, and good water quality. Beneficial effects

[0029] 1. By regulating the influent ammonia-nitrogen concentration under the conditions of controlling pH and temperature, adding the short-cut nitrite-ammonia oxidizing bacterium NZ-AY1 provided by the present invention can achieve a rapid start of the short-cut nitrification process. This process can operate stably for a long time, laying a foundation for the simultaneous nitrification and denitrification or anaerobic ammonia oxidation process of high-ammonia-nitrogen wastewater, thereby achieving complete denitrification and having a wide application prospect in the field of sewage treatment.

[0030] 2. The present invention provides the short-cut nitrifying bacterium Geobacillus toebii ( Parageobacillus toebii ) NZ-AY1. This Geobacillus toebii ( Parageobacillus toebii ) NZ-AY1 can carry out short-cut nitrification under autotrophic and aerobic conditions, has a fast growth rate, strong metabolic ability, an ammonia-nitrogen removal efficiency of over 95%, an ammonia oxidation efficiency of 87% to 95%, and strong resistance to various shock loads. It is more stable in the treatment system, can operate stably for a long time, and is not easily lost and decayed.

[0031] 3. The present invention provides the short-cut nitrifying bacterium Geobacillus toebii ( Parageobacillus toebii ) NZ-AY1, and its ammonia-nitrogen conversion efficiency of short-cut nitrification can reach over 95%, which is more conducive to achieving a rapid start of short-cut nitrification and providing technical support for simultaneous nitrification and denitrification or anaerobic ammonia oxidation. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 is the process flow block diagram of the process of the present invention;

[0033] Figure 2 is the change curve of ammonia-nitrogen, nitrate-nitrogen, and nitrite-nitrogen concentrations in the experiment of rapid start of short-cut nitrification of ammonia-oxidizing bacterium NZ-AY1 in high-ammonia-nitrogen wastewater;

[0034] Figure 3 is the change curve of the conversion rates of nitrate-nitrogen and nitrite-nitrogen in the experiment of rapid start of short-cut nitrification of ammonia-oxidizing bacterium NZ-AY1 in high-ammonia-nitrogen wastewater;

[0035] Figure 4 is the change curve of the degradation rate of ammonia-nitrogen with the dosage in the treatment of actual ammonia-nitrogen-containing wastewater by ammonia-oxidizing bacterium NZ-AY1

[0036] Figure 5 It is the concentration change curves of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen in the actual ammonia-nitrogen-containing wastewater treated by ammonia-oxidizing bacteria NZ-AY1;

[0037] Figure 6 It is the concentration change curves of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen in the actual ammonia-nitrogen-containing wastewater treated by adding ordinary activated sludge. Embodiment

[0038] The following examples are applicable to the present invention but do not limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.

[0039] In the following examples, the determination method of ammonia nitrogen is: Nessler reagent spectrophotometry; the determination method of nitrite nitrogen: ultraviolet spectrophotometry; the determination method of nitrate nitrogen is: thymol spectrophotometry.

[0040] Example 1 Obtaining of ammonia-oxidizing bacterial strain NZ-AY1

[0041] The strain was screened from the bottom mud of a river in Taizhou. The specific screening method is as follows:

[0042] Using NH 4 Cl 0.4 g, KH 2 PO 4 0.08 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.02 g, CaCl 2 ·2H 2 O 0.01 g, trace element solution 1 mL / L (zinc chloride 80 mg, anhydrous copper sulfate 20 mg, boric acid 20 mg, ferrous sulfate heptahydrate 100 mg), water 1000 mL, pH 7.8 - 8.5) as the screening medium, and adding 50 mL of river mud taken from a river in Taizhou. After 20 days, the ammonia nitrogen was degraded to 0.1 g / L, and the nitrite nitrogen accumulation reached 0.25 g / L. Take the supernatant and inoculate it into the aerobic ammonia-oxidizing medium ((NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 20.1 g, trace element solution 1 mL / L (zinc chloride 80 mg, anhydrous copper sulfate 20 mg, boric acid 20 mg, ferrous sulfate heptahydrate 100 mg), water 1000 mL, pH 7.8 - 8.5), and cultured in the medium until the bacterial concentration reaches 1*10 8 CFU / mL.

[0043] The strain is elliptical, milky yellow, with a capsule, no spores, Gram-positive, heterotrophic, strictly aerobic, and positive for ammonia nitrogen nitrification test.

[0044] The culture conditions of the strain are as follows: Take 0.1 mL of the bacterial solution and evenly spread it on the medium ((NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 2 O 0.1 g, FeSO 4 ·7H 2 O 0.001 g, agar 20 g), and place the medium in a constant temperature incubator for culturing at a temperature of 33 °C for 5 days.

[0045] This strain can grow in wastewater with an ammonia nitrogen concentration of 300 - 500 mg / L, with a doubling period of 5 days, and the effective viable count can reach 10 9 CFU / ml or more.

[0046] Example 2 Cultivation of ammonia-oxidizing bacterium NZ-AY1

[0047] Bacillus paralicheniformis ( Parageobacillus toebii ) NZ-AY1 was used for strain cultivation, including activation, transfer, and expansion steps, as follows:

[0048] 1) Activation: Pick a single colony of ammonia-oxidizing bacteria from the solid plate and transfer it to the ammonia-oxidizing liquid medium. The shaker speed is 130 rpm, and culture in a shaker at 33 °C until the logarithmic phase;

[0049] 2) Transfer: Transfer the ammonia-oxidizing bacterial solution in the logarithmic phase to a seed tank for cultivation. Control the temperature of the seed tank to be maintained at 33 °C, the rotation speed to be kept at 230 rpm, and the dissolved oxygen DO to be controlled at 5.5 mg / L, and culture for 5 days;

[0050] 3) Amplification culture: The bacterial liquid cultured in the seed tank was transferred to the fermentation tank for amplification culture at an inoculation amount of 5%. The components of the fermentation tank medium were the same as those of the seed tank. The physical and chemical parameters were as follows: temperature 33 °C, rotation speed 230 rpm, dissolved oxygen 5.5 mg / L, and fermentation time 3 d.

[0051] The components of the ammonia-oxidizing liquid medium are: (NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 2 O 0.1 g, trace element solution 1 mL / L (zinc chloride 80 mg, anhydrous copper sulfate 20 mg, boric acid 20 mg, ferrous sulfate heptahydrate 100 mg), water 1000 mL, pH 7.8 - 8.5.

[0052] The medium components of the seed tank are: (NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 2 O 0.1 g, FeSO 4 ·7H 2 O 0.001 g, agar 20 g, trace element solution 1 mL / L, water 1000 mL, pH 7.8 - 8.5.

[0053] After the strain is cultured, the effective viable count of the bacterial liquid can reach more than 10 9 CFU / mL. After the fermentation culture liquid is taken out of the tank and packaged, a high-efficiency ammonia-oxidizing bacterial agent can be obtained.

[0054] Example 3: Short-cut nitrification rapid start experiment of ammonia-oxidizing bacteria NZ-AY1 in high ammonia-nitrogen wastewater

[0055] In this example, self-prepared ammonia-nitrogen wastewater was used. In 30 L of tap water, 45.84 g of NH 4 Cl was added to make the initial ammonia-nitrogen concentration 400 mg / L. In addition, other nutrient elements required for ammonia-oxidizing bacteria need to be added: 0.55 g of KH 2 PO 4 and 0.6 g of MgSO4 ·7H 2 O, 0.3 g CaCl 2 , 15 mL of trace elements, using 10% NaHCO 3 solution to adjust the pH of the wastewater to 7.8, adjust the influent temperature to 33 °C, and the ammonia nitrogen concentration to 400 mg / L. Add the adjusted wastewater to a biological aeration reactor, keep the dissolved oxygen below 6 mg / L, add 5% of the highly efficient degrading bacteria screened by the present invention, and measure the concentrations of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen in the effluent at microbial residence times of 6 h, 12 h, 24 h, 48 h, and 72 h respectively. The data is as shown in the appendix Figure 2 shown. After 24 h of operation, the rapid start-up of shortcut nitrification is completed. After 72 h of operation, the ammonia nitrogen degradation rate reaches 99.9%, and the nitrite nitrogen conversion rate reaches 95%. To further confirm the feasibility of the rapid start-up process of shortcut nitrification, adjust the influent ammonia nitrogen concentration, and measure the concentrations of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen in the effluent every 1 d, and calculate the conversion rates of nitrate nitrogen and nitrite nitrogen. The data is as shown in the appendix Figure 3 shown. The results show that the rapid start-up process of shortcut nitrification for high ammonia nitrogen wastewater operates stably for one month, and its nitrite nitrogen conversion rate is 87 - 95%, with good results.

[0056] Example 4: Application of the inoculation amount of ammonia-oxidizing bacteria NZ-AY1 in the rapid start-up of shortcut nitrification in actual high ammonia nitrogen wastewater

[0057] To further verify the application effect of the ammonia-oxidizing bacteria NZ-AY1 used in the present invention in the start-up of shortcut nitrification in actual ammonia nitrogen-containing wastewater, a shortcut nitrification rapid start-up experiment was carried out on the breeding wastewater of a certain farm in Yancheng, Jiangsu. Its main pollutant is ammonia nitrogen. After measurement, the ammonia nitrogen concentration in the wastewater is 500 mg / L. Add the fermentation broth of ammonia-oxidizing bacteria NZ-AY1 directly to the ammonia nitrogen-containing breeding wastewater at inoculation amounts of 2.5%, 5%, 10%, 15%, and 20%, appropriately supplement the phosphorus source KH 2 PO 4 and trace elements and adjust the system pH to be controlled between 7.0 - 7.8. Under the aeration condition of 33 degrees, sample and monitor the changes of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen in the system after 24 h. The data is as shown in the appendix Figure 4 shown. The monitoring results show that: under different dosages of ammonia-oxidizing bacteria NZ-AY1, shortcut nitrification can be achieved. However, when the dosage is 2.5%, the ammonia nitrogen degradation efficiency is low, only decreasing from 500 mg / L to 450 mg / L, and the degradation effect is 10%. When the dosage of ammonia-oxidizing bacteria NZ-AY1 is 10%, the degradation rate can reach 65%. Continuing to increase the dosage does not significantly increase the degradation efficiency. The above experimental data shows that ammonia-oxidizing bacteria NZ-AY1 can achieve rapid shortcut nitrification and reach the best ammonia nitrogen degradation efficiency when the dosage is 10%.

[0058] Example 5: Application of ammonia-oxidizing bacterium NZ-AY1 in treating aquaculture wastewater

[0059] To further verify the application effect of the ammonia-oxidizing bacterium NZ-AY1 used in the present invention in the short-cut nitrification startup of actual ammonia-nitrogen-containing wastewater, the aquaculture wastewater from a farm in Yancheng, Jiangsu was used for a short-cut nitrification rapid startup experiment. Its main pollutant is ammonia-nitrogen. After measurement, the ammonia-nitrogen concentration in the wastewater is 500 mg / L. The fermentation broth of the ammonia-oxidizing bacterium NZ-AY1 was directly added to the ammonia-nitrogen-containing aquaculture wastewater at an inoculation amount of 10%. An appropriate amount of phosphorus source KH 2 PO 4 and trace elements were supplemented, and the pH of the system was adjusted to be between 7.0 and 7.8. Under the aeration condition at 33 degrees, samples were taken regularly at 0 h, 6 h, 12 h, 24 h, and 48 h to monitor the changes of ammonia-nitrogen, nitrate-nitrogen, and nitrite-nitrogen in the system. The data are as shown in Appendix Figure 5 . The results show that the ammonia-nitrogen in the wastewater began to degrade within 6 h. After 12 h, the ammonia-nitrogen concentration had dropped to 150 mg / L, and the degradation rate reached 70%. After 24 h, the ammonia-nitrogen concentration had dropped to 34 mg / L, and the degradation rate was as high as 93%. The degradation of the above ammonia-nitrogen basically occurred in the form of conversion to nitrite-nitrogen. During the whole monitoring process, the concentration of nitrate-nitrogen was lower than 10 mg / L.

[0060] Comparative Example 1:

[0061] To further verify the application effect of the ammonia-oxidizing bacterium NZ-AY1 used in the present invention in the short-cut nitrification startup of actual ammonia-nitrogen-containing wastewater, the aquaculture wastewater from a farm in Yancheng, Jiangsu was used for a short-cut nitrification rapid startup experiment. Its main pollutant is ammonia-nitrogen. After measurement, the ammonia-nitrogen concentration in the wastewater is 500 mg / L. Instead of using the highly efficient degrading bacteria screened in the present invention, ordinary activated sludge from the farm was added for microbial short-cut nitrification reaction. An appropriate amount of phosphorus source KH 2 PO 4 and trace elements were supplemented, and the pH of the system was adjusted to be between 7.0 and 7.8. Under the aeration condition at 33 degrees, samples were taken regularly at 0 h, 6 h, 12 h, 24 h, and 48 h to monitor the changes of ammonia-nitrogen, nitrate-nitrogen, and nitrite-nitrogen in the system. The data are as shown in Appendix Figure 6 . The results show that the degradation of ammonia-nitrogen in the wastewater occurred in the form of conversion to nitrate-nitrogen, and the short-cut nitrification reaction could not occur.

Claims

1. An ammonia-oxidizing bacterium, classified and named as Geobacillus subterraneus subsp. in-situ composting strain ( Parageobacillus toebii ) NZ-AY1, has been deposited in the China General Microbiological Culture Collection Center with the deposit number: CGMCC No. 26235.

2. Use of the ammonia-oxidizing bacterium according to claim 1 in a rapid start-up process for shortcut nitrification of wastewater with a high ammonia-nitrogen concentration, wherein the ammonia-nitrogen concentration in the wastewater with a high ammonia-nitrogen concentration is 300 mg / L - 500 mg / L.

3. According to the use described in claim 2, characterized in that: After culturing the strain of Geobacillus toebii ( Parageobacillus toebii ), NZ-AY1, it is added to the wastewater with a relatively high concentration of ammonia nitrogen, and the temperature is controlled at 30-33 °C and the pH at 7.0-8.5 to achieve a rapid start-up process of shortcut nitrification.

4. According to the use described in claim 3, characterized in that: Subterraneibacillus in-situ composting strain ( Parageobacillus toebii ) NZ-AY1 was cultured, including activation, transfer, and amplification steps: Activation: Pick a single colony of ammonia-oxidizing bacteria from a solid plate, transfer it to an ammonia-oxidizing medium, and culture it on a shaker at a rotational speed of 130 - 160 rpm and a temperature of 30 - 33 °C until the logarithmic phase; Transfer: Transfer the ammonia-oxidizing bacteria solution in the logarithmic phase to a seed tank for culture, control the temperature of the seed tank to be maintained at 30 - 33 °C, the rotational speed to be maintained at 220 - 250 rpm, and the dissolved oxygen DO to be controlled at 4 - 6 mg / L, and culture for 5 d - 10 d; Expansion culture: Transfer the bacterial solution cultured in the seed tank to a fermentation tank for expansion culture according to an inoculation amount of 2% - 5%. The composition of the fermentation tank medium is the same as that of the seed tank, and the physical and chemical parameter indexes are: temperature 30 - 33 °C, rotational speed 230 - 260 rpm, dissolved oxygen 4 - 6 mg / L, and fermentation time 3 - 5 d.

5. According to the use described in claim 4, characterized in that: The components of the ammonia oxidation medium: (NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 2 O 0.1 g, 1 mL of trace element solution, 1000 mL of water, pH 7.8 - 8.

5.

6. According to the use described in claim 4, characterized in that: The culture medium components of the seed tank are: (NH 4 ) 2 SO 4 2.0 g, KH 2 PO 4 1.0 g, NaHCO 3 1 g, MgSO 4 ·7H 2 O 0.2 g, CaCl 2 ·2H 2 O 0.1 g, FeSO 4 ·7H 2 O 0.001 g, agar 20 g, 1 mL of trace element solution, water 1000 mL, pH 7.8 - 8.

5.

7. According to the use described in claim 4, characterized in that: After culturing, the effective viable count of the bacterial liquid of the strain can reach 10 9 CFU / ml or more. After the fermentation culture broth is discharged from the tank and packaged, a highly efficient ammonia-oxidizing bacteria agent can be obtained.

8. According to the use described in claim 5 or 6, characterized in that: The 1 mL trace element solution contains 80 mg of zinc chloride, 20 mg of anhydrous copper sulfate, 20 mg of boric acid, and 100 mg of ferrous sulfate heptahydrate.

9. According to the use described in claim 3, characterized in that: The dosage of the ammonia-oxidizing bacterium NZ-AY1 is 2% - 10%.

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