An in situ tissue specimen anaerobic culture bottle and its inoculation method

Abstract

The application discloses an intraoperative tissue specimen anaerobic culture bottle and a seeding method thereof, and aims at solving the problems of complicated early processing steps, time waste and specimen pollution during processing in the prior art, which affects the authenticity of results. The application comprises a bottle body, an accommodation cavity is arranged on the upper part of the bottle body along the height direction, and a culture cavity is arranged on the lower part of the bottle body along the height direction; an inner cover is arranged at the joint of the accommodation cavity and the culture cavity, and a cylindrical outer cover is arranged in the accommodation cavity; during use, the inner cover is closed, the outer cover is used to grind the tissue specimen, the inner cover is opened after grinding, the slurry culture solution falls into the culture cavity, the inner cover is closed, and sealed culture is carried out; the application has the advantages of simple structure and operation steps, convenient use, time saving, direct specimen processing and inoculation after the specimen is taken out during operation, less pollution of the tissue specimen, improved inoculation positive rate of the tissue specimen, and improved authenticity of results.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial culture, in particular to an intraoperative tissue specimen anaerobic bacteria culture bottle and a inoculation method thereof. BACKGROUND

[0002] Anaerobic bacteria, also known as anaerobic bacteria, are bacteria that grow better in anaerobic conditions than in aerobic environments, and cannot grow on the surface of solid culture medium under 18% oxygen and / or 10% carbon dioxide concentration. It can cause infection in different parts of the human body, including appendicitis, cholecystitis, otitis media, oral infection, endocarditis, endometritis, brain abscess, myocardial necrosis, osteomyelitis, peritonitis, empyema, salpingitis, septic arthritis, liver abscess, sinusitis, wound infection after intestinal surgery or trauma, pelvic inflammatory disease, pressure ulcers, diabetic foot, and bacteremia. Anaerobic bacteria identification requires transportation, processing and culture in special environment. Since the environment where anaerobic bacteria survive needs to be strictly controlled, any mistake in any link will bring serious error to the culture result.

[0003] The current clinical operation is to take the tissue specimen in the operation, then put the tissue into a sterile bottle or an anaerobic transport bottle, and then the specimen is transported to the microbiology room by the doctor after the operation; the microbiological examination technician inoculates the tissue specimen transported after the preliminary processing. In this operation process, the tissue specimen needs to be stored in the conventional environment for a long time, and the anaerobic bacteria in the tissue may be inactivated in the oxygen-rich environment. Even if there is an anaerobic special storage device, the staff still needs to further inoculate after the preliminary processing (grinding) of the tissue specimen, which leads to complicated operation steps, time waste, high cost, and the possibility of contaminating the specimen during processing. The anaerobic bacteria in the tissue may be inactivated in the oxygen-rich environment, thereby affecting the authenticity of the result. SUMMARY

[0004] Therefore, the present application provides an intraoperative tissue specimen anaerobic bacteria culture bottle and a inoculation method thereof to solve the problems of complicated preliminary processing steps, time waste, and the possibility of contaminating the specimen during processing, which affects the authenticity of the result.

[0005] In order to achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0006] The first aspect is an anaerobic bacteria culture bottle for intraoperative tissue specimen, comprising: a bottle body, an upper part of the bottle body is provided with a containing cavity in the height direction, and a lower part of the bottle body is provided with a culture cavity in the height direction, the volume of the culture cavity is greater than the volume of the containing cavity, the containing cavity is used for storing and grinding the tissue specimen, and the culture cavity is used for sealing culture of the ground mud-like culture solution; an inner cover is arranged at the joint of the containing cavity and the culture cavity, the containing cavity and the culture cavity are communicated after the inner cover is opened; a cylindrical outer cover is arranged in the containing cavity, and the outer cover corresponds to the upper part of the inner cover.

[0007] Optionally, the bottle body is in a cylindrical structure; and the containing cavity and the culture cavity are circular cavities.

[0008] Optionally, the height of the containing cavity is less than the height of the culture cavity, and the diameter of the containing cavity is less than the diameter of the culture cavity.

[0009] Optionally, the top end of the outer cover is provided with a cross-shaped notch, and an outer force is applied to the outer cover by a wrench matched with the cross-shaped notch, so that the outer cover grinds the tissue specimen in the containing cavity.

[0010] Optionally, the outer wall of the outer cover is connected to the inner wall of the upper part of the bottle body in a threaded manner and corresponds to the containing cavity.

[0011] Optionally, the inner cover is connected to the bottle body in a pulling manner.

[0012] Optionally, the inner cover is connected to the bottle body in a rotating manner.

[0013] Optionally, a sterile vacuum packaging bag is further included, and the culture bottle is placed in the sterile vacuum packaging bag.

[0014] The second aspect is a method for inoculating intraoperative tissue specimen, using the above-mentioned anaerobic bacteria culture bottle for intraoperative tissue specimen, comprising the following steps:

[0015] Step one, first open the sterile vacuum packaging bag, place the culture bottle on the operating table, and take the tissue specimen suspected of being infected during the operation;

[0016] Step two, cut the tissue specimen into tissue fragments using tissue scissors;

[0017] Step three, open the outer cover, put the tissue fragments into the containing cavity, add physiological saline into the containing cavity, and cover the outer cover;

[0018] Step four, tighten the outer cover in the containing cavity, so that the bottom of the outer cover grinds the tissue fragments in the containing cavity;

[0019] Step five, after the tissue pieces are ground into mud slurry culture solution, the inner cover is opened, so that the culture solution falls into the culture cavity, and then the inner cover is closed;

[0020] Step six, after the operation, the culture bottle containing the culture solution is sent to the microbial incubator for microbial culture.

[0021] Compared with the prior art, the present application has at least the following beneficial effects:

[0022] 1. The present application provides an in-situ tissue specimen anaerobic bacteria culture bottle and its inoculation method. The culture bottle comprises a bottle body, a containing cavity is arranged on the upper part of the bottle body along the height direction, a culture cavity is arranged on the lower part of the bottle body along the height direction, the volume of the culture cavity is larger than that of the containing cavity, an inner cover is arranged at the joint of the containing cavity and the culture cavity, the containing cavity and the culture cavity are communicated after the inner cover is opened, a cylindrical outer cover is arranged in the containing cavity, and the outer cover corresponds to the upper part of the inner cover. In use, the inner cover is closed, the outer cover is rotated in the containing cavity to grind the tissue specimen, the inner cover is opened after the grinding is completed, so that the ground mud slurry culture solution falls into the culture cavity, and then the inner cover is closed. The slurry culture solution is cultured in the culture cavity in a sealed manner. The structure and operation steps of the present application are simple, convenient to use, time-saving, and reduce the transportation of the tissue and the pre-treatment of the specimen. After the tissue specimen is taken out in-situ, the specimen is directly treated and inoculated, the tissue specimen is not easily contaminated, the positive rate of bacterial culture of the tissue specimen is improved, and the true results are provided for guiding the clinical treatment, thereby benefiting the patients.

[0023] 2. The present application is also provided with a wrench and a sterile vacuum packaging bag. The wrench is matched with the outer cover, the outer cover is pressed, the outer cover is quickly rotated into the containing cavity, and the tissue pieces in the containing cavity are ground. The sterile vacuum packaging bag is used for packaging the culture bottle, and the culture bottle can be directly placed on the operating table after the packaging is opened.

[0024] 3. The inner cover and the bottle body of the present application are connected in a pull-out manner, so that the mud slurry culture solution can be quickly and conveniently dropped. DETAILED DESCRIPTION

[0025] In order to more directly illustrate the prior art and the present application, several exemplary drawings are given below. It should be understood that the specific shapes, structures shown in the drawings should not be regarded as the limiting conditions for realizing the present application; for example, based on the technical concept disclosed in the present application and the exemplary drawings, those skilled in the art can easily make routine adjustments or further optimization on the increase / decrease / attribute division of certain units (components), specific shapes, positional relationship, connection mode, size ratio relationship, etc.

[0026] Figure 1 The structure diagram provided by the first embodiment of the present application Figure 1 ;

[0027] Figure 2 As shown in the exploded schematic view; Figure 1

[0028] Figure 3 As shown in the exploded schematic view; Figure 2 As shown in the schematic view of the structure of the inner cover in the closed state;

[0029] Figure 4 As shown in the schematic view of the structure of the inner cover in the closed state; Figure 2 As shown in the schematic view of the structure of the inner cover in the closed state;

[0030] Figure 5 As shown in the schematic view of the structure of the inner cover in the closed state; Figure 2 As shown in the schematic view of the structure of the inner cover in the closed state;

[0031] Figure 6 As shown in the schematic view of the structure of the inner cover in the closed state;

[0032] Figure 7 As shown in the schematic view of the structure of the inner cover in the closed state;

[0033] Figure 8 As shown in the schematic view of the structure of the inner cover in the closed state;

[0034] Explanation of reference signs:

[0035] 1, bottle body; 11, accommodating cavity; 12, culture cavity; 13, insertion slot; 131, first insertion slot; 132, second insertion slot; 2, outer cover; 21, cross-shaped notch; 3, inner cover; 31, convex strip; 4, wrench; 41, cross-shaped protrusion. DETAILED DESCRIPTION

[0036] The application will be further described in detail below with reference to the accompanying drawings.

[0037] In the description of the application: unless otherwise specified, the meaning of "multiple" is two or more. The terms "first", "second", "third" and the like in the application are intended to distinguish the objects referred to, and do not have a special meaning in the technical connotation aspect (for example, it should not be understood as emphasizing importance or order, etc.). The expressions such as "include", "contain", "have" and the like also mean "not limited to" (some units, components, materials, steps, etc.).

[0038] The terms such as "upper", "lower", "left", "right", "intermediate" and the like in the application are generally used for intuitive understanding with reference to the drawings, and are not an absolute limitation on the positional relationship in the actual product. Without departing from the technical concept disclosed in the application, the change of these relative positional relationships is also considered as the scope of the description of the application.

[0039] Example One​

[0040] An in-situ tissue specimen anaerobic culture bottle, as shown in the drawings, comprises a bottle body 1 in a cylindrical structure, a receiving cavity 11 is arranged at the center of the upper part of the bottle body 1 in the height direction, a culture cavity 12 is arranged at the lower part in the height direction, the volume of the culture cavity 12 is greater than that of the receiving cavity 11, the receiving cavity 11 is used for storing and grinding the tissue specimen, and the culture cavity 12 is used for sealed culture of the ground mud-like culture solution; Figures 1-5

[0041] The culture cavity 12 stores the culture solution, the receiving cavity 11 is communicated with the culture cavity 12, and an inner cover 3 is arranged at the joint of the receiving cavity 11 and the culture cavity 12; a columnar outer cover 2 is arranged in the receiving cavity 11, and the outer cover 2 corresponds to the upper part of the inner cover 3;

[0042] In the embodiment, a wrench 4 and a sterile vacuum packaging bag are also arranged, the wrench 4 is matched with the outer cover 2, can press the outer cover 2, and can quickly rotate the outer cover 2 into the receiving cavity 11 to grind the tissue specimen (tissue fragments) in the receiving cavity 11; the sterile vacuum packaging bag is used for packaging the culture bottle, and when the mud-like culture solution falls into the culture bottle for culture, the culture bottle is placed in the sterile vacuum packaging bag and then is transported to a microbial culture box;

[0043] As shown in the drawings, the inner cover 3 is connected with the bottle body 1 in a pulling manner; as shown in the drawings, a slot 13 is arranged in the bottle body 1 in the transverse direction, the slot 13 is located at the bottom position of the receiving cavity 11, the slot 13 comprises a first slot 131 and a second slot 132 communicated with the first slot 131, the first slot 131 is recessed into the bottle body 1 at the position close to the bottom end of the lower part of the receiving cavity 11, the second slot 132 is a strip, one end of the second slot 132 is communicated with the first slot 131, and the other end of the second slot 132 extends to the outer wall of the bottle body 1 and is communicated with the outside; the height of the second slot 132 is consistent with that of the first slot 131; the inner cover 3 is a sheet-shaped body matched and inserted with the above-mentioned slot 13, the outer side of the sheet-shaped body is provided with an arc-shaped protruding strip 31 abutting with the outer wall of the bottle body 1, the transverse width of the protruding strip 31 is greater than that of the slot 13, and the pulling of the inner cover 3 is facilitated; Figure 2 Figure 4 When the tissue specimen in the receiving cavity 11 needs to be ground, the inner cover 3 is inserted into the slot 13 to seal the bottom of the receiving cavity 11, so that the upper end surface of the inner side of the inner cover 3 and the bottom end surface of the outer cover 2 form a certain cavity capable of accommodating the tissue specimen; when the mud-like culture solution in the receiving cavity 11 needs to fall into the culture cavity 12, an operator holds the protruding strip 31 of the inner cover 3 and pulls it outwards, so that the receiving cavity 11 is communicated with the culture cavity 12 at the lower part.

[0044]

[0045] ​​​Preferably, the accommodating cavity 11 and the culture cavity 12 are circular cavities; the height of the accommodating cavity 11 is less than the height of the culture cavity 12, and the diameter of the accommodating cavity 11 is less than the diameter of the culture cavity 12.

[0046] Preferably, the total height of the bottle body 1 is 8-9 cm; the height of the accommodating cavity 11 is 2-3 cm, and the diameter is 3 cm; the diameter of the culture cavity 12 is 6 cm, and the above-mentioned size values can be adjusted according to actual conditions.

[0047] Preferably, as shown in Figure 2 , Figure 3 , the wrench 4 is an L-shaped wrench, one end of the wrench 4 is provided with a cross-shaped protrusion 41, and the other end serves as a hand holding part; the top end of the outer cover 2 is provided with a cross-shaped notch 21, which is matched with the cross-shaped protrusion 41 on the wrench 4, and the outer cover 2 is ground against the tissue specimen in the accommodating cavity 11 by applying an external force to the outer cover 2 through the hand holding part.

[0048] Preferably, the outer wall of the outer cover 2 is connected to the inner wall of the upper part of the bottle body 1 in a threaded manner and corresponds to the accommodating cavity 11.

[0049] Example Two

[0050] As shown in Figures 6-8 , example two is basically the same as example one, except that the connection mode of the inner cover 3 and the bottle body 1 is different; in example two, the inner cover 3 and the bottle body 1 are connected in a rotating manner, the bottle body 1 is provided with an accommodating cavity 11 along the height direction, and the accommodating cavity 11 is not arranged at the center position of the bottle body 1, but is arranged on the outer peripheral surface, which is eccentrically arranged, as shown in Figure 8 ; the center position of the bottle body 1 is provided with a rotating shaft (not shown in the figure), the inner side of the inner cover 3 is rotationally connected with the rotating shaft, and the outer end of the inner cover 3 is provided with an arc-shaped protruding strip 31 which is attached to the outer wall of the bottle body 1; the bottle body 1 is provided with a slot 13 which is matched with the inner cover 3.

[0051] A method for inoculating an intraoperative tissue specimen, which adopts the above-mentioned anaerobic bacteria culture bottle for intraoperative tissue specimen, comprising the following steps:

[0052] Step one, first open the sterile vacuum packaging bag, place the culture bottle on the operating table, and take the suspected infected tissue specimen (soft tissue) during the operation; for example, a soft tissue with a diameter of 2 cm can be taken;

[0053] Step two, cut the tissue specimen into tissue fragments using tissue scissors; the tissue fragments can be cut into 0.5 cm fragments;

[0054] Step three, close the inner cover 3, open the outer cover 2, put the cut tissue pieces into the containing cavity 11, then add two drops of normal saline into the containing cavity 11, and cover the outer cover 2; the adding amount of normal saline is adjusted according to the amount of the tissue pieces put into the containing cavity 11;

[0055] Step four, tighten the outer cover 2 into the containing cavity 11 by the L-shaped wrench 4, so that the bottom of the outer cover 2 grinds the tissue pieces in the containing cavity 11;

[0056] Step five, after the tissue pieces are ground into mud slurry culture solution, open the inner cover 3, so that the mud slurry culture solution in the containing cavity 11 falls into the culture cavity 12, then close the inner cover 3, and take away the wrench 4;

[0057] Step six, after the operation, send the culture bottle containing the mud slurry culture solution to the microbial incubator for microbial culture.

[0058] The technical features of the above embodiments can be combined in any manner (as long as the combination of the technical features does not contradict), in order to make the description simple, not all possible combinations of the technical features in the above embodiments are described; the embodiments not explicitly written should also be considered as the scope of the present application.

[0059] The application is described in detail above by general description and specific embodiments. It should be understood that, based on the technical concept of the present application, some conventional adjustments or further innovations can be made to the specific embodiments; however, as long as the technical concept of the present application is not deviated, the technical solutions obtained by these conventional adjustments or further innovations also fall within the protection scope of the claims of the present application.

Claims

1. A culture flask for anaerobic bacteria in intraoperative tissue specimens, characterized in that, include: The bottle body has a receiving cavity at the top along the height direction and a culture cavity at the bottom along the height direction. The volume of the culture cavity is larger than that of the receiving cavity. The receiving cavity is used to store and grind tissue specimens, and the culture cavity is used to seal and culture the ground mud-like culture medium. An inner cover is provided at the junction of the receiving cavity and the culture cavity. The inner cover is connected to the bottle body by a pull-out or rotation type. After opening the inner cover, the receiving cavity and the culture cavity are connected. A columnar outer cover is provided inside the receiving cavity, and the outer cover is located above the inner cover.

2. The intraoperative tissue specimen anaerobic bacteria culture bottle according to claim 1, characterized in that, The bottle body has a cylindrical structure; the accommodating cavity and the culture cavity are circular cavities.

3. The intraoperative tissue specimen anaerobic bacteria culture bottle according to claim 2, characterized in that, The height of the accommodating cavity is less than the height of the culture cavity, and the diameter of the accommodating cavity is less than the diameter of the culture cavity.

4. The intraoperative tissue specimen anaerobic culture bottle according to claim 1 or 3, characterized in that, The top of the outer cover is provided with a cross-shaped notch, and an external force is applied to the outer cover by a wrench that is adapted to the cross-shaped notch, so that the outer cover grinds the tissue specimen in the accommodating cavity.

5. The intraoperative tissue specimen anaerobic bacteria culture bottle according to claim 4, characterized in that, The outer wall of the outer cap is connected to the inner wall of the upper part of the bottle body by a thread and corresponds to the receiving cavity.

6. The intraoperative tissue specimen anaerobic bacteria culture bottle according to claim 1, characterized in that, It also includes a sterile vacuum packaging bag, into which the culture flask is placed.

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