A method for rapid and stable identification of storage tolerance of batch rice seeds

Abstract

The present invention relates to the field of cultivation technology, and specifically provides a method for rapidly and stably identifying the storage tolerance of batch rice, comprising the following steps: placing multiple rice seeds to be tested in an aging box in order after packaging, placing multiple layers, multiple rows in each layer, multiple positions in each row, and placing a pair of seeds in each position; the day when the seeds are placed in the box is recorded as the 0th day of treatment, and the seeds in the box are moved in a timely and orderly manner on the 1st day and the subsequent double days, and after the aging is completed, the germination effect of each rice seed to be tested is measured, and the storage tolerance of the rice seeds is identified according to the germination effect. The method of the present invention is a key technology for subsequent phenotypic identification of genetic populations for storage-resistant gene positional cloning, or for rapidly screening storage-resistant materials from a large number of resources or breeding materials.

Description

Technical Field

[0001] The invention relates to a method for quickly and stably identifying the storage resistance of batch rice seeds in the technical field of plant breeding. Background Art

[0002] How to reduce storage costs and ensure that rice seeds have a high germination rate after being stored for about two years is an important scientific issue. To this end, it is necessary to screen and create storage-resistant resources and materials, locate and clone storage-resistant related genes, and develop tightly linked markers to breed new storage-resistant varieties.

[0003] In the above screening and breeding process, a large number of rice storage tolerance identifications are required. The rice storage tolerance identification methods are divided into multi-year natural aging identification methods and artificial aging identification methods. Among them, the multi-year natural aging identification method takes a long time. The currently reported artificial aging identification method for a large number of seeds is fast, but has the problem of poor repeatability. Summary of the invention

[0004] In order to replace the time-consuming multi-year natural aging identification method and the existing artificial aging identification method with poor repeatability, the problem to be solved by the present invention is how to quickly and stably perform batch rice storage tolerance identification and screening.

[0005] In order to solve the above problems, the present invention provides a method for rapidly and stably identifying the storage resistance of batches of rice seeds, comprising the following steps: a plurality of rice seeds to be tested are packaged and placed in an aging box in sequence for aging, with N layers, M rows per layer, Y groups per row, X positions per group, and a pair of seeds placed in each position; the day on which the seeds are placed in the box is recorded as the 0th day of treatment, and the seeds in the box are moved in order once on the 1st day and the even-numbered days thereafter, and the manner of each movement is as follows:

[0006] Layer: The bottom layer before each move becomes the top layer after the move, and all other layers move down one layer;

[0007] Rows: Before each move, the rightmost row becomes the leftmost row, and all other rows move one row to the right;

[0008] Position: Move the last pair of seeds in each group to become the first seed in the group, and move the other pairs of seeds back one position;

[0009] N, M, X, and Y are positive integers greater than or equal to 2;

[0010] After aging is completed, the germination effect of each rice seed to be tested is measured, and the storage resistance of the rice seeds is identified based on the germination effect;

[0011] The rice to be tested includes storage-resistant control rice and non-storage-resistant control rice.

[0012] In the above method, it is preferred that the positions of the same pair of seeds are swapped when the positions are moved for the Xth time, and the positions of the same pair of seeds are swapped again when the positions are moved for the 2Xth time.

[0013] In the above method, the germination effect can be determined based on the number of broken seeds on the 4th day after germination and / or the live germination rate on the 7th day after germination, preferably the live germination rate on the 7th day after germination.

[0014] In the above method, the packaging is packaging in nylon seed bags of 6 cm×8 cm, and the placement is hanging.

[0015] In the above method, the aging temperature is 42° C. and the relative humidity is 85%.

[0016] In the above method, the seeds have a moisture content of 12%-13% before packaging. After packaging, the seeds are placed in a forced air drying box for 4 days before entering the aging box. The seeds are dried at 45-50°C during the day (50°C for seeds stored within 2 months, and 45°C for seeds stored after 2 months), with the ventilation holes opened for 10 hours, and the ventilation holes are closed for 14 hours at room temperature at night.

[0017] In the above method, the aging box is set to 42°C / RH85% (temperature is 42°C, relative humidity is 85%) one day before the seeds are put in, a thermometer is placed inside, and the aging box is run empty to confirm that the temperature displayed by the aging box is the same as the temperature data on the thermometer.

[0018] In the above method, the aging box can be selected from the seed aging box LH-150S (150L), LH-250S (250L) and the like produced by Zhejiang Top Yunnong Technology Co., Ltd.

[0019] In the above method, N, M, X, and Y are set according to the capacity specifications of the aging box. For example, when using the seed aging box LH-150S (150L), N=4, M=3, X×Y=8, and the number of seeds is N×M×X×Y×2=192; when using the seed aging box LH-250S (250L), N=4, M=4, X×Y=10, and the number of seeds is N×M×X×Y×2=320.

[0020] When the sample of rice to be tested is less than N×M×X×Y×2 (for example, in one embodiment of the present invention, 4×3×4×2×2=192), it is supplemented with storability control rice and non-storage-resistant control rice.

[0021] In the above method, the storable control rice is a rice variety whose germination effect after aging under the method (temperature of 42°C and relative humidity of 85%) is greater than or equal to 84% of the live germination rate on the 7th day of germination. The specific storable control rice can be selected from LV7436 (Plant New Variety Application No. 20211007264), P122, etc.

[0022] In the above method, the non-storage-resistant control rice is a rice variety whose germination effect after aging under the method (temperature of 42° C., relative humidity of 85%) is less than or equal to 35% of the live bud rate on the 7th day of germination. The specific non-storage-resistant control rice can be selected from Xiangwanxian No. 13 (Xiangpinshen No. 308, hereinafter referred to as XWX13), Yuzhenxiang (Xiangshendao 2009038), and Nongxiang 42 (Xiangshendao 2016019).

[0023] In the above method, the aging and treatment days are determined according to the indica and japonica types of the test seeds, the number of days of natural storage after harvest, the storage-resistant control rice and the non-storage-resistant control rice. The longer the number of days of natural storage after harvesting the seeds, the shorter the number of days of artificial aging treatment. Generally, indica rice seeds that are naturally stored for 60 days (inclusive) need to be treated for 25 days, and japonica rice seeds for 20 days; indica rice seeds that are naturally stored for 60 days (excluding) to 90 days (inclusive) need to be treated for 22 days, and japonica rice seeds for 15 days; indica rice seeds that are naturally stored for 90 days (excluding) to 180 days (inclusive) need to be treated for 15 days, and japonica rice seeds for 10 days.

[0024] The present invention also protects the use of the above method for identifying the storage resistance of batch rice seeds in screening storage-resistant rice; in the application, the rice to be tested whose germination effect is no different from or higher than that of the storage-resistant control rice is the storage-resistant rice.

[0025] The invention discloses a method for rapidly and stably screening storable rice in batches, which is a key technology for subsequent phenotypic identification of genetic groups for positional cloning of storability genes, or for rapidly screening storability materials from a large number of resources or breeding materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a photo of the seed bag in Example 1 of the present invention hanging in the aging box. Figure 1 Figure A is a photo of 4 layers of seeds hanging in the aging box. Figure 1 Figure B is a photograph of each layer of hanging seeds.

[0027] Figure 2 The diagram is a sample movement position diagram when the sample enters the aging box (treatment day 0) and during the treatment period of 25 days in Example 1 of the present invention. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0029] The quantitative tests in the following examples were all repeated three times, and the results were averaged.

[0030] The experimental methods in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.

[0031] The following examples use Microsoft Excel 2003 software to process and plot data, and SPSS10.0 analysis software to test the data for significant differences. The experimental results are expressed as mean ± standard deviation, P < 0.05 (*) indicates a significant difference, and P < 0.01 (**) indicates an extremely significant difference.

[0032] The plant variety application number of the storage-resistant control rice variety LV7436 in the following examples is 20211007264, and P122 is a variety from the Hunan Crop Germplasm Resource Bank, which the public can obtain from the applicant Hunan Rice Research Institute to repeat the experiment of this application.

[0033] The non-storage-tolerant control rice variety Xiangwanxian 13 (hereinafter referred to as XWX13) in the following examples was approved by Hunan in 2001, with the number: Xiangpinshen No. 308; the approval number of Yuzhenxiang is Xiangshendao 2009038; the approval number of Nongxiang 42 is Xiangshendao 2016019. The above materials can be obtained by the public from the applicant Hunan Rice Research Institute to repeat the experiments of this application.

[0034] Example 1

[0035] 1. A method for rapid and stable detection of storage tolerance of batch rice

[0036] The specific method is as follows:

[0037] 1. Harvest seeds. Harvest seeds 30-35 days after the first heading and pack them in nylon seed bags.

[0038] 2. Dry the seeds. After harvesting, dry them until the moisture content is 12%-13% (dry the seeds in the sun for several consecutive days, or dry them in a 45℃ forced air drying oven). The dried seeds are placed in yellow paper bags for use.

[0039] 3. Select seeds that are full, not cracked, without disease spots, and yellow and ripe.

[0040] 4. Package the seeds. Use 6 cm x 8 cm nylon seed bags (small mesh bags) to pack the seeds, with 50 seeds in each bag; clamp every 2 bags together with a dovetail clip. Use varieties with a live bud rate greater than or equal to 84% after aging under the method and conditions of the invention as storage-resistant control rice (LV7436, P122, etc. can be selected, and P122 is selected in this embodiment) as storage-resistant control rice, and use varieties with a live bud rate less than or equal to 35% after aging under the method and conditions of the invention as non-storage-resistant control rice (XWX13, Yuzhenxiang, or Nongxiang 42, etc. can be selected, and XWX13 is selected in this embodiment). Clamp 1 bag of storage-resistant control rice and 1 bag of non-storage-resistant control rice together with a dovetail clip.

[0041] 5. Balanced drying of seeds. Four days before entering the aging box, the bagged seeds are placed in a forced air drying box for four days, with the temperature at 45-50°C (50°C for seeds stored in the seed collection room within three months, and 45°C for seeds stored indoors after three months), with the ventilation holes opened for 10 hours during the day, and the ventilation holes closed for 14 hours at room temperature at night.

[0042] 6. Test run of aging box. Use a seed aging box purchased from Zhejiang Top Yunnong Technology Co., Ltd., with optional models such as LH-150S (150L), LH-250S (250L), etc. This embodiment uses LH-150S (150L), which has 4 shelves. One day before the aging box is injected, set it to 42°C / RH85%, place a thermometer inside, run it empty, and confirm that the temperature displayed by the aging box is the same as the temperature data on the thermometer.

[0043] 7. Seeds are placed in an aging box. After the seeds have been balanced and dried for 4 days (40 hours in total), they are placed in an aging box that has been tested and operated normally. Every 2 bags are clamped together with a dovetail clip and placed in a hanging manner. One box and one batch contain 192 samples (96 pairs) (see Figure 1 ), the aging box has 4 layers (marked as A layer, B layer, C layer, and D layer from top to bottom), each layer has 3 rows (marked as a row, b row, and c row from left to right), each row has 8 pairs of 16 samples in total (8 positions, the one near the aging box door is the 8th position, and 1 pair of samples occupies 1 position), each row of row a, b row, and c row of layer A has 1 storability control rice and 1 non-storage-resistant control rice, the storability control rice and non-storage-resistant control rice in row a of layer A are placed in the 1st position of the row (near the inner wall of the aging box), the storability control rice and non-storage-resistant control rice in row b of layer A are placed in the 5th position of the row, and the storability control rice and non-storage-resistant control rice in row c of layer A are placed in the 8th position of the row (near the door of the aging box). There are 8 pairs of 16 samples (including controls) in each row, and they are marked as 1, 2, 3, 4, ..., 16 in the order of the day they were placed in the box (recorded as treatment day 0). See the diagram for the placement of each sample. Figure 2 The position of day 0 in .

[0044] 8. Determination of the number of days of aging. The day the seeds are put into the box is recorded as the 0th day of treatment, the day after the aging treatment is completed for 24 hours is recorded as the 1st day, and the day after the treatment is completed for 48 hours is recorded as the 2nd day. A total of 10 days, 15 days, 22 days, or 25 days of treatment are carried out. The number of days of treatment is determined according to the indica and japonica types of the test seeds, the number of days of natural storage after harvest, the storage-resistant control rice, and the storage-intolerant control rice. The longer the number of days of natural storage after harvesting the seeds, the shorter the number of days of artificial aging treatment. Generally, indica rice seeds that are naturally stored for 60 days (inclusive) need to be treated for 25 days, and japonica rice seeds for 20 days; indica rice seeds that are naturally stored for 60 days (not included) to 90 days (inclusive) are treated for 22 days, and japonica rice seeds for 15 days; indica rice seeds that are naturally stored for 90 days (not included) to 180 days (inclusive) are treated for 15 days, and japonica rice seeds for 10 days. This embodiment is treated for 25 days.

[0045] 9. Cyclic movement. On the first day (after 24 hours of processing), the seeds in the box should be moved in a timely and orderly manner. On the next two days (the second day, the fourth day, and the sixth day), the seeds in the box should be moved in a timely and orderly manner. See the position change diagram for each move. Figure 2 , specifically:

[0046] Layer: The bottom layer before each move becomes the top layer after the move, and the other layers move down one layer in sequence;

[0047] Rows: Before each move, the rightmost row becomes the leftmost row, and the other rows move one row to the right in sequence;

[0048] Position: Each row is divided into 2 groups, positions 1-4 in one group and positions 5-8 in another group. Before moving, the last pair of samples in each group becomes the first pair of samples in the group, and the other pairs are moved back one position in sequence.

[0049] The 4th time (the Xth time, Figure 2 Day 6) Move the positions of the same pair of seeds and swap their positions (see Figure 2 Bold part in the middle font), until the 8th time (2Xth time, Figure 2 On the 14th day in the experiment, the positions of the same pair of seeds are swapped again (i.e., changed back) while the positions are moved.

[0050] When moving seeds, first move all the racks and seeds out of the box and move them quickly to prevent large fluctuations in temperature and humidity inside the box.

[0051] 10. Seed germination. On the day of treatment, close the aging box, pour the aged seeds into a germination box lined with filter paper, add water to half the thickness of the seeds; place in a light incubator at 30°C, 12 hours of light / 12 hours of dark conditions for 7 days.

[0052] 11. Data determination.

[0053] The day when the seeds were placed in the light incubator was day 0. The number of seeds with broken cores was recorded on day 4. The number of live buds, dead buds or deformed buds, and unbroken seeds was recorded on day 7. The live bud rate and broken core rate were calculated.

[0054] Broken heart rate = 100% × number of broken heart seeds on the 4th day of germination / total number of treated seeds

[0055] Survival bud rate = 100% × number of surviving buds on the 7th day of germination / total number of treated seeds

[0056] The total number of treated seeds = number of living buds + number of dead or deformed buds + number of unbroken seeds.

[0057] 2. Verification of the results of the same model aging box

[0058] 3 aging boxes of the same model LH-150S (produced in Hangzhou Top).

[0059] From the offspring of P122 and XWX13, 166 strains with good agronomic traits or storage resistance were selected, each with 3 bags of 50 grains. There were 39 bags of 50 grains each for the storage-resistant control rice P122 and the non-storage-resistant control rice XWX13.

[0060] Each box was filled with 192 samples (including 13 samples each of the storable control rice P122 and the non-storable control rice XWX13, and 166 samples of the F7 lineage seeds of the hybrid combination of P122 and XWX13). When the seeds were put into the boxes, the storable and non-storable control rice were placed in the first position (first row) of row a of layer A, the fifth position (middle row) of row b of layer A, and the eighth position (last row) of row c of layer A; because there were only 166 samples of the F7 lineage seeds of the hybrid combination of P122 and XWX13, and the box could hold 192 samples, i.e., 8 pairs in 1 row (N=4, M=3, X=4, Y=2), 10 pairs of controls were added, and the storable control rice and the non-storable control rice were placed in the eighth position (last row) of row a of layer A, rows a, b, c of layer B, rows a, b, c of layer C, and rows a, b, c of layer D.

[0061] The results are shown in Table 1:

[0062] Table 1 Correlation analysis results of heart rate on day 4

[0063]

[0064] Table 2 Correlation analysis results of live bud rate on the 7th day

[0065]

[0066] Table 3 Correlation analysis results between the heart-breaking rate on the 4th day and the live bud rate on the 7th day

[0067]

[0068]

[0069] Table 4 Calculation results of the difference in heart rate between the two control varieties on the 4th day

[0070]

[0071] Table 5 Calculation results of the difference in live bud rate between two control varieties on the 7th day

[0072]

[0073]

[0074] The results of correlation analysis showed that after 25 days of aging treatment, the heart-breaking rate of seeds on the 4th day of germination was significantly positively correlated with that of seeds on the 7th day of germination (Table 1), and the live bud rate of seeds on the 7th day of germination was significantly positively correlated with that of seeds on the 7th day (Table 2). The heart-breaking rate on the 4th day was significantly positively correlated with the live bud rate on the 7th day (Table 3). There were 13 samples of storage-resistant control rice P122 and non-storage-resistant control rice XWX13 in the same box. The results of variance analysis showed that the heart-breaking rate of P122 on the 4th day was significantly higher than that of XWX13 (Table 4), and the live bud rate of P122 on the 7th day was significantly higher than that of XWX13 (Table 5). The P value between the repeated measurements of the heart-breaking rate on the 4th day of seeds was 0.005 (Table 4), while the P value between the repeated measurements of the live bud rate on the 7th day was 0.0003 (Table 5), indicating that the results on the 7th day can better reflect individual differences and are relatively reliable. Based on the results of correlation analysis and variance analysis, the live bud rate on the 7th day after aging treatment was determined as the criterion for judging storage resistance.

[0075] III. Comparison of the consistency between the results of 16 indica rice varieties treated in the same aging chamber and the natural aging or storage-resistant gene marker detection

[0076] To verify the effect of the above method, 16 materials with known storage resistance results were used for verification. The 16 research materials used were selected based on the results of natural aging over many years by this research group and the results of the hybridization of P122 and XWX13 with molecular markers of SAT1 storage resistance gene developed by this research group in cooperation with the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. A total of 16 varieties (lines) were selected. The names and sources and other related information are shown in Table 6:

[0077] Table 6 Relevant information of the 16 varieties used

[0078]

[0079]

[0080] LV7436 was used as the storage-tolerant control rice, and XWX13 was used as the storage-intolerant control rice.

[0081] In 2022, we will breed seeds at the Sanya base. From mid-April to the end of April, we will harvest mature seeds according to the initial heading period and dry them in time. Changsha Selected Packaging, each material package contains 12 packets of seeds, 12 replicates per variety, and 50 seeds per package.

[0082] 30 days after harvest (May 20), drying and dormancy removal treatment (50°C for 40 hours) before storage began. When storage, 16 varieties of each repetition were hung in pairs in the same row, 8 pairs in a row, 3 rows in a layer, 4 layers in total (N=4, M=3, X=4, Y=2), that is, 1 variety had 12 repetitions. At 42°C and 85% RH, the positions were moved according to the above rules, and the moving method was exactly the same as "I. Method for rapid and stable detection of storage resistance of batch rice".

[0083] Treat for 25 days; then place on paper bed for germination, at 30℃, 8000LUX light for 12 hours, no light for 12 hours for germination for 7 days, and test the germination rate on the 7th day. The results are shown in Table 7 and Table 8:

[0084] Table 7 Results of live bud rate on the 7th day

[0085]

[0086]

[0087] Table 8 Differences among treatment varieties

[0088] Sample No. Variety name deal with Mean 5% significant level 1% extremely significant level 1 LV7436 Process 1 88.33％ b B 2 8MAGIC276 Process 2 69.00％ e D 3 P105 Process 3 95.50％ a A 4 P122 Process 4 96.67％ a A 5 GUI630 Process 5 94.50％ a A 6 KATY Process 6 77.33％ c C 7 NX39 Process 7 35.50％ h G 8 NX42 Process 8 26.33％ i H 9 XWX13 Process 9 25.50％ i H 10 YZX Processing 10 20.50％ j I 11 AS30 Process 11 17.83％ j I 12 22BJ11 Processing 12 51.00％ f E 13 22BJ12 Process 13 73.50％ d CD 14 8MAGIC55 Processing 14 89.50％ b B 15 22BJ5-2 Processing 15 40.33％ g F 16 22BJ15 Processing 16 96.00％ a A

[0089] The results showed that the difference in live bud rate between 12 replicates of the same variety was within the maximum allowable difference between four replicates of the same test specified in the National Crop Seed Inspection Procedure (GB / T3543.4-1995) (Table 7); according to the average live bud rate of artificial aging, ≥84% was storable, and ≤35% was not storable. The artificial aging storability results of 16 varieties were consistent with the natural aging results or storability marker detection results (Table 6, Table 7); variance analysis showed that the difference in live bud rate after artificial aging among varieties reached a significant or extremely significant level (Table 8). The above shows that this method can identify the difference in storability among varieties.

[0090] 4. Comparison between the paper bag flat in the static box and the small mesh bag hanging and moving regularly

[0091] The names, sources, and planting and harvesting periods of the 16 research material seeds are the same as those in the above “III. Comparison of the consistency of the treatment results of the 16 varieties in the same aging box with the natural aging or storage-resistant gene marker detection”. Selected packages are divided into 12 packages of seeds per material package, 12 replicates per variety, and 50 seeds per package.

[0092] The samples were processed in two aging boxes, with the samples in one box hanging and the samples in the other box lying flat:

[0093] The method of hanging treatment is exactly the same as “3. Comparison of the consistency of treatment results of 16 varieties in the same aging box with natural aging or storage-resistant gene marker detection”.

[0094] The flat-laying treatment is to replace the 6 cm high × 8 cm long nylon seed bag with a 6 cm wide × 8 cm long parchment seed bag, and change the hanging placement method to laying it flat in sequence at the corresponding position of each layer and each row. The position does not move during the treatment process. Other treatment methods are the same as "3. Comparison of the consistency of treatment results of 16 varieties in the same aging box with natural aging or storage-resistant gene marker detection."

[0095] Before the seeds are put into the box, the temperature, humidity and accuracy of the two aging boxes are calibrated together to confirm that the conditions in the two boxes are consistent when the setting is 42°C and 85%. The results are shown in Table 9:

[0096] Table 9: The survival rate of seeds aged in paper bags and spread out in a static box after aging on the 7th day

[0097]

[0098] The results showed that the germination rate of the seeds aged in paper bags and laid flat in the aging box on the 7th day had a large range among 12 repetitions, which was greater than the maximum allowable difference between four repetitions of the same germination test (National Crop Seed Inspection Procedure GB / T3543.4-1995) (Table 9), indicating that the aging results of the same variety of materials in different positions in the box exceeded the allowable difference, and the experimental results were unreliable. The main reason is that when there are many seeds in the box, when they are laid flat for many days, if there is water mist and water droplets, they will drop directly on the paper seed bag in the same position many times. The humidity treatment of this seed is different from that of other seeds, resulting in a low germination rate on the 7th day.

[0099] Sixteen varieties were treated by a method of laying paper bags flat and an experimental method of placing seeds in small mesh bags, hanging them in pairs, and moving the seeds regularly during the aging period. Comparative analysis of the experimental results (Table 7 and Table 9) showed that the average values ​​of the live bud rates of the 16 varieties on the 7th day after aging had the same trend between the two methods, but the range (variability) of the same variety between 12 repetitions in the method of laying paper bags flat was greater than that in the method of hanging them with mesh belts of the present invention, indicating that the results of the aging treatment according to the present invention are more scientific and effective.

[0100] The present invention has been described in detail above. It will be apparent to those skilled in the art that the present invention may be implemented in a wide range under equivalent parameters, concentrations and conditions without departing from the spirit and scope of the present invention and without the need for unnecessary experimentation. Although the present invention provides specific embodiments, it should be understood that further improvements may be made to the present invention. In short, according to the principles of the present invention, this application intends to include any changes, uses or improvements to the present invention, including changes made by conventional techniques known in the art that depart from the scope disclosed in this application. Applications of some of the basic features may be made within the scope of the following appended claims.

Claims

1. A method for identifying the storage tolerance of batch rice seeds, characterized in that: The steps include: After multiple rice seeds to be tested are packaged, they are placed in an aging box in order for aging. They are placed in N layers, M rows in each layer, Y groups in each row, X positions in each group, and a pair of seeds in each position. The day when the seeds are placed in the box is recorded as the 0th day of treatment. On the 1st day and the even-numbered days thereafter, the seeds in the box are moved in order once. The method of each movement is as follows: Layer: The bottom layer before each move becomes the top layer after the move, and all other layers move down one layer; Rows: Before each move, the rightmost row becomes the leftmost row, and all other rows move one row to the right; Position: Move the last pair of seeds in each group to become the first seed in the group, and move the other pairs of seeds back one position; N, M, X, and Y are positive integers greater than or equal to 2; After aging is completed, the germination effect of each rice seed to be tested is measured, and the storage resistance of the rice seeds is identified based on the germination effect; The rice to be tested includes a storage-resistant control rice and a non-storage-resistant control rice; When the position is moved for the Xth time, the positions of the same pair of seeds are swapped, and when the position is moved for the 2Xth time, the positions of the same pair of seeds are swapped again; The germination effect is determined based on the live bud rate on the 7th day of germination; The packaging is to pack the seeds in nylon seed bags of 6 cm×8 cm, and the placement is to hang the seeds.

2. The method according to claim 1, characterized in that: The aging is carried out in an aging box at a temperature of 42° C. and a relative humidity of 85%.

3. The method according to claim 1 or 2, characterized in that: The seeds have a moisture content of 12%-13% before packaging. After packaging, the seeds are placed in a forced air drying box for 4 days before entering the aging box. The seeds are dried with the ventilation holes opened at 45-50° C. for 10 hours during the day and with the ventilation holes closed for 14 hours at room temperature at night.

4. The method according to claim 3, characterized in that: The storage-resistant control rice is a rice whose germination effect after aging is that the live germination rate on the 7th day after germination is greater than or equal to 84%.

5. The method according to claim 3, characterized in that: The storage-intolerant control rice is a rice whose germination effect after aging is that the live germination rate on the 7th day after germination is less than or equal to 35%.

6. Use of the method according to any one of claims 1 to 5 in screening storability rice; in said use, the rice to be tested whose germination effect is no different from or higher than that of the storability control rice is the storability rice.

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