Composition with natural antiseptic properties and preparation method and application thereof

Abstract

The present invention discloses a composition with natural antiseptic properties, which is composed of the following components by mass percentage: 0.1% to 2% lysozyme, 0.01% to 2% hydrolyzed collagen, 0.01% to 1% sodium chloride, 0.01% to 2% tea extract, 10% to 30% propylene glycol, 0.01% to 1% grapefruit seed extract, 0.01 to 0.02% tridecapeptide-1, the remainder is water, and the sum of the mass percentages of the above components is 100%. The composition solves the problem that the irritation caused by traditional preservatives in cosmetics is large and the antibacterial effect is not thorough. A preparation method of a composition with natural antiseptic properties and the application of the composition with natural antiseptic properties in cosmetics are also disclosed.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and specifically relates to a composition with natural antiseptic properties, a preparation method of the composition with natural antiseptic properties, and application of the composition with natural antiseptic properties in cosmetics. Background Art

[0002] In order to maintain product quality during the production and use of cosmetics, adding appropriate amounts of preservatives will not only not harm human health, but can also inhibit or kill microorganisms to a certain extent, which is an effective means to ensure product quality and safety. Due to safety concerns, the frequency of use of paraben preservatives, which were the most frequently used in the early days, in new products has rapidly decreased in recent years, while the frequency of use of alcohol preservatives with low allergenicity, such as phenoxyethanol preservatives, has increased significantly. Traditional preservatives will accumulate in the stratum corneum of the skin, promote skin aging, accelerate damage to skin cell DNA, lead to imperfect stratum corneum formation, and hinder the effectiveness of beauty ingredients. Therefore, the less preservatives added, the better, while inhibiting the growth of microorganisms.

[0003] Cosmetics are susceptible to microbial contamination during production and use, so different types of preservatives must be added to cosmetics to prevent the proliferation of microorganisms. The main reason for cosmetic deterioration is microbial contamination. There are a large number of various microorganisms around us. The main ways of microbial contamination in cosmetics include primary contamination during the production process and secondary contamination caused by consumers when using them. Among them, we have effectively controlled the number of microorganisms in the product through disinfection and sterilization methods during the production of cosmetics, and better avoided primary contamination caused by equipment, operating processes, packaging and storage. However, when the product meets the anti-corrosion standards and is strictly and correctly packaged, the product can be better preserved, but during use, the product will be contaminated for the second time. Secondary contamination is also called contamination during use, which is often caused by not paying attention to hygiene during use. For example, every time it is used, the microorganisms on the hands or tools are brought into the product during the process of taking it, causing microbial contamination. Furthermore, since the organic components in cosmetics (such as lipids, light and water-soluble polymers, etc.) not only have abundant carbon sources, nitrogen sources and other nutrients required for the growth and reproduction of microorganisms, but also the oxidation of compounds can provide chemical energy for their reproduction, as long as there is a suitable environment, the microorganisms in the product will grow and reproduce rapidly, causing the product to spoil. Common microbial flora in cosmetics include bacteria (such as golden Staphylococcus aureus, Enterobacterium hominis, Pseudomonas, etc.), yeasts (such as Candida albicans, Candida) and molds (such as Aspergillus niger, Aspergillus niger, etc.).

[0004] In recent years, proteins and peptides have attracted great attention as antimicrobial agents as an antibiotic-free approach to treat bacterial infections. Naturally occurring antimicrobial peptides and enzymes have the advantages of high activity, broad antimicrobial spectrum, and low bacterial resistance to these drugs. Summary of the invention

[0005] The first object of the present invention is to provide a composition with natural antiseptic properties, which solves the problems of high irritation and incomplete antibacterial effect caused by traditional preservatives in cosmetics.

[0006] The second object of the present invention is to provide a method for preparing a composition having natural antiseptic properties.

[0007] The third object of the present invention is to provide a composition with natural antiseptic properties for use in cosmetics.

[0008] The first technical solution adopted by the present invention is that the composition with natural preservative performance is composed of the following components in mass percentage: 0.1% to 2% lysozyme, 0.01% to 2% hydrolyzed collagen, 0.01% to 1% sodium chloride, 0.01% to 2% tea extract, 10% to 30% propylene glycol, 0.01% to 1% grapefruit seed extract, 0.01% to 0.02% tridecapeptide-1, and the balance is water, and the sum of the mass percentages of the above components is 100%.

[0009] The second technical solution adopted by the present invention is a method for preparing a composition with natural preservative properties. At room temperature, propylene glycol and water are first mixed evenly, and then lysozyme, hydrolyzed collagen, sodium chloride, tea extract, grapefruit seed extract and tridecapeptide-1 are added in sequence, and stirred evenly to obtain the composition.

[0010] The third technical solution adopted by the present invention is the application of the above-mentioned composition with natural antiseptic properties in cosmetics.

[0011] The present invention is also characterized in that

[0012] The cosmetic product is lotion.

[0013] Cosmetics are lotions or creams.

[0014] The beneficial effects of the present invention are:

[0015] The hydrolyzed collagen in the composition of the present invention can improve the antibacterial activity of lysozyme and increase the antibacterial property of lysozyme. Lysozyme can also increase the efficacy of collagen and prolong the usability of collagen. The role of sodium chloride in this compound is to make the whole system more stable. The compounding of lysozyme and tea extract can increase the antibacterial activity, and the tea extract has certain antioxidant properties. Grapefruit seed extract has strong antioxidant properties in addition to antibacterial function, which can enhance the antioxidant capacity of cosmetics and prevent other components from being oxidized; the role of propylene glycol is to dissolve it into liquid, which has a solubilizing effect and also has a certain moisturizing effect. Lysozyme, tridecapeptide-1, and hydrolyzed collagen all have a certain promoting effect on the activity of lysozyme, achieving the effect of 1+1+1>3, and greatly improving the efficacy of a single raw material itself through synergistic effect. Compared with traditional preservatives, it has the characteristics of naturalness and safety, and can be applied to a series of products such as water, lotion, and cream. The composition can be applied to most cosmetics such as lotions, creams and ointments, and can effectively inhibit gram-negative bacteria, gram-positive bacteria, yeast, black aspergillus and the like. DETAILED DESCRIPTION

[0016] The present invention is described in detail below in conjunction with specific implementation modes.

[0017] The invention provides a composition with natural antiseptic performance. The composition consists of the following components by mass percentage: 0.1% to 2% lysozyme, 0.01% to 2% hydrolyzed collagen, 0.01% to 1% sodium chloride, 0.01% to 2% tea extract, 10% to 30% propylene glycol, 0.01% to 1% grapefruit seed extract, 0.01% to 0.02% tridecapeptide-1, and the balance is water, and the sum of the mass percentages of the above components is 100%.

[0018] The effects of each raw material in the composition of the present invention are as follows:

[0019] (i) Lysozyme is a natural antimicrobial agent that is abundant in tears, saliva, breast milk and mucus.

[0020] Lysozyme can inhibit certain Gram-positive bacteria by degrading the 1,4-b bond between N-acetyl-D-glucosamine and N-acetyl-muramic acid in the bacterial cell wall peptidoglycan. It can kill Gram-positive (G+) bacteria. In addition to hydrolyzing peptidoglycan, it can also induce bacteria to produce lysozyme to kill Gram-positive (G+) bacteria. In addition to hydrolyzing peptidoglycan, it can also induce bacteria to produce autolytic enzymes. It can stimulate microorganisms to produce autolytic enzymes through non-enzymatic mechanisms to sterilize. The enzyme can strongly decompose G+ bacteria such as Bacillus subtilis and radioresistant Micrococcus. Some lysozymes also have a certain dissolving effect on G- bacteria such as Escherichia coli, common mutans and Vibrio parahaemolyticus. Egg white lysozyme has antibacterial effects on both Gram-positive and Gram-negative bacteria. Due to its significant properties such as antibacterial, antihistamine, antiviral, antibacterial, and antitumor, lysozyme is of great significance in the fields of pharmacology and biomedicine. In particular, research on the antibacterial effect of lysozyme is becoming more and more popular. Lysozyme is a simple alkaline globulin composed of 18 kinds of 129 amino acids, with a molecular weight of 14.4KDa and very stable chemical properties. When the pH value changes drastically in the range of 1.2 to 11.3, its structure remains almost unchanged. In an acidic environment, lysozyme is very stable to heat and can still maintain the activity of the original enzyme. Its pure product is white or slightly yellow. It is a yellow crystal or amorphous powder, odorless and sweet. It is easily soluble in water, easily destroyed by alkali, and insoluble in acetone and ether. As a medical product, lysozyme has the advantages of good biocompatibility, no irritation to tissues, and no toxicity.

[0021] Lysozyme also has the following advantages: (1) It has good antimicrobial efficacy, including antibacterial and antifungal. (2) It can achieve good antimicrobial efficacy with a small amount. (3) It has a wide pH range of use. (4) It has good safety, is non-toxic and non-irritating. (5) It is chemically inert and will not react with ingredients or packaging materials other than those in the formula. (6) It has a more suitable distribution coefficient between the oil phase and the water phase, so that the preservative can have an effective preservative effect in the oil phase and the water phase of the product. (7) It has good compatibility and can be compatible with most raw materials without changing the color and smell of the final product. (8) It has a low cost of use and is easy to obtain. (9) It is a raw material that is allowed to be added by national laws and regulations and has no regulatory risks. Therefore, it is very necessary to provide a natural compound preservative with high safety that can meet consumers' pursuit of green, environmentally friendly and safe cosmetics. This compound also has a certain moisturizing effect.

[0022] (2) Hydrolyzed collagen contains hydrophilic natural moisturizing factors and triple helix structure. It can strongly lock in moisture and protect the skin to maintain a moist and supple state. The moisturizing property of hydrolyzed collagen is quite good, so using it can maintain the moisture content of the skin, thereby maintaining the skin's luster. The molecules of hydrolyzed collagen are small and are very easily absorbed by the skin. After absorption, the hydrolyzed collagen is filled into the dermis of the skin, helping to increase the firmness of the skin, generate skin tension, shrink the pores of the skin, and make the skin tight and elastic. The dermis containing a plump layer of hydrolyzed collagen can support the skin cells, and at the same time, combined with the moisturizing property of hydrolyzed collagen itself, it can achieve the effect of fading fine lines and stretching coarse lines. Active hydrolyzed collagen can penetrate into the bottom layer of the skin very well and has good affinity with the surrounding tissues, so it can help cells to produce hydrolyzed collagen and promote the normal growth of cells in the skin.

[0023] (III) Tea extract can penetrate deep into the skin and maintain high activity, effectively inhibiting the production of melanin, and is an efficient, safe, and green whitening and anti-freckle cosmetic ingredient. Tea extract has the effects of preventing skin roughness and anti-inflammatory and antibacterial, and has antibacterial and anti-inflammatory, UV protection, skin whitening, and freckle removal.

[0024] (IV) Sodium chloride is usually dissolved in water and glycerin, but not easily dissolved in ethanol. Its chemical properties are relatively stable. It mainly serves the purpose of viscosity control in products. Its main function in cosmetics and daily chemical products is antibacterial effect.

[0025] (V) Propylene glycol is a widely used polyol that can help dissolve dirt and prevent the skin from drying out too quickly during makeup removal. It can also help active ingredients penetrate and retain moisture in the stratum corneum, thus having a moisturizing effect.

[0026] (VI) Grapefruit seed extract is a product obtained through extraction and processing using grapefruit seeds as raw materials. It is a colorless to light yellow liquid with a bitter taste. The main components are sugars, fatty acids and flavonoid compounds. It is natural, safe and non-toxic, has no irritation or allergic reaction, has a broad spectrum of antibacterial properties, has a good inhibitory effect on a variety of bacteria, fungi and viruses, and is conducive to the growth of the body's own beneficial bacteria. Grapefruit seed extract also has strong antioxidant properties, which can enhance the antioxidant capacity of cosmetics and prevent other components from being oxidized.

[0027] (VII) Tridecapeptide-1 has a broad-spectrum antibacterial activity and has a certain inhibitory effect on Staphylococcus aureus, Pseudomonas aeruginosa, yeast, Escherichia coli, and Candida albicans.

[0028] The present invention also provides a method for preparing the above-mentioned composition with natural antiseptic properties: at room temperature, propylene glycol and water are first mixed evenly, and then lysozyme, hydrolyzed collagen, sodium chloride, tea extract, grapefruit seed extract and tridecapeptide-1 are added in sequence, and stirred evenly to obtain the composition.

[0029] The present invention also provides the use of the composition with natural antiseptic properties in cosmetics, wherein the cosmetics are lotions, emulsions or creams.

[0030] The components involved in the composition in the embodiments of the present invention were all purchased through commercial channels: lysozyme was purchased from Meimu (Beijing) Technology Co., Ltd., tea extract was purchased from Ganzhou Huahan Biotechnology Co., Ltd., hydrolyzed collagen was purchased from Tianjin Kezhiqi Technology Co., Ltd., sodium chloride was purchased from Sinopharm Chemical Reagent Co., Ltd., propylene glycol and grapefruit seed extract were purchased from Dow Chemical (Shanghai) Co., Ltd.

[0031] Embodiment 1

[0032] Add 2g of lysozyme, 30g of propylene glycol, 2g of hydrolyzed collagen, 1g of sodium chloride, 2g of tea extract, 1g of grapefruit seed extract, and 0.02g of tridecapeptide-1, add water to 100g, mix well, and obtain a transparent composition with natural preservative properties.

[0033] Embodiment 2

[0034] 0.8 g of lysozyme, 18 g of propylene glycol, 0.18 g of hydrolyzed collagen, 0.15 g of sodium chloride, 0.13 g of tea extract, 0.15 g of grapefruit seed extract, and 0.01 g of tridecapeptide-1 were added, water was added to 100 g, and the mixture was thoroughly mixed to obtain a transparent composition with natural preservative properties.

[0035] Embodiment 3

[0036] 0.6 g of lysozyme, 16 g of propylene glycol, 0.13 g of hydrolyzed collagen, 0.13 g of sodium chloride, 0.12 g of tea extract, 0.1 g of grapefruit seed extract, and 0.01 g of tridecapeptide-1 were added, water was added to 100 g, and the mixture was thoroughly mixed to obtain a transparent composition with natural preservative properties.

[0037] Embodiment 4

[0038] 0.5 g of lysozyme, 12 g of propylene glycol, 0.1 g of hydrolyzed collagen, 0.1 g of sodium chloride, 0.08 g of tea extract, 0.05 g of grapefruit seed extract, and 0.01 g of tridecapeptide-1 were added, water was added to 100 g, and the mixture was thoroughly mixed to obtain a transparent composition with natural preservative properties.

[0039] Embodiment 5

[0040] 0.1 g of lysozyme, 10 g of propylene glycol, 0.01 g of hydrolyzed collagen, 0.01 g of sodium chloride, 0.01 g of tea extract, 0.01 g of grapefruit seed extract, and 0.01 g of tridecapeptide-1 were added, water was added to 100 g, and the mixture was thoroughly mixed to obtain a transparent composition with natural preservative properties.

[0041] The preparation method of the composition with natural antiseptic properties in Examples 1 to 5 is as follows: at room temperature, propylene glycol and water are first mixed evenly, and then lysozyme, hydrolyzed collagen, sodium chloride, tea extract, grapefruit seed extract and tridecapeptide-1 are added in sequence, and stirred evenly to obtain the composition.

[0042] Corrosion test

[0043] In order to prove the effectiveness of this composition, the composition with natural antiseptic properties in the example was used in the following formula of an essence water.

[0044] 1. Example 1 Test

[0045] Table 1.1 Application of the composition in Example 1

[0046]

[0047]

[0048] The above essence water was subjected to an antiseptic challenge test.

[0049] Anticorrosion test method: Inoculate suitable bacteria (bacteria-Aureus stippling, yeast-Candida albicans, mold-Aspergillus niger) into the culture medium and culture them at 37°C / 28°C / 28°C for 48 hours. Select certain fungi to make a fungus suspension, weigh a sample of the same mass as the composition in Example 1 and add it to the fungus suspension. The number of fungi is required to be roughly the same. After fully mixing, take samples and analyze them at 0d, 1d, 7d, 14d, 28d, and 60d under 28°C.

[0050]

[0051] Table 1.2 Experimental results of a certain essence water

[0052]

[0053]

[0054] Conclusion: From the test results in Table 1.2, it can be seen that the essence water was tested for fungal growth on the 7th, 14th, 28th and 60th days after inoculation. There was no growth of fungi on the 7th day and no growth within 60 days. All passed the antiseptic challenge test, indicating that the compound with natural antiseptic properties of the present invention has strong antibacterial properties. The prepared essence water is not susceptible to secondary contamination during storage and use, and the physical and chemical performance indicators meet the requirements of relevant quality standards.

[0055] 2. Test of Example 2

[0056] Table 2.1 Application of the composition in Example 2

[0057]

[0058]

[0059] See 1. Example 1 Test for anti-corrosion test methods and evaluation criteria

[0060] Table 2.2 Experimental results of a certain essence water

[0061]

[0062] Conclusion: From the test results in Table 2.2, it can be seen that the essence water was tested for fungal growth on the 7th, 14th, 28th and 60th days after inoculation. There was no growth of fungi on the 7th day and no growth within 60 days. All passed the antiseptic challenge test, indicating that the compound with natural antiseptic properties of the present invention has strong antibacterial properties. The prepared essence water is not susceptible to secondary contamination during storage and use, and the physical and chemical performance indicators meet the requirements of relevant quality standards.

[0063] 3. Test of Example 3

[0064] Table 3.1 Application of the composition in Example 3

[0065]

[0066]

[0067] See 1. Example 1 Test for anti-corrosion test methods and evaluation criteria

[0068] Table 3.2 Experimental results of a certain essence water

[0069]

[0070]

[0071] Conclusion: From the test results in Table 3.2, it can be seen that the essence water was tested for fungal growth on the 7th, 14th, 28th and 60th days after inoculation. There was no growth of fungi on the 7th day and no growth within 60 days. All passed the antiseptic challenge test, indicating that the compound with natural antiseptic properties of the present invention has strong antibacterial properties. The prepared essence water is not susceptible to secondary contamination during storage and use, and the physical and chemical performance indicators meet the requirements of relevant quality standards.

[0072] 4. Test of Example 4

[0073] Table 4.1 Application of the composition in Example 4

[0074]

[0075]

[0076] See 1. Example 1 Test for anti-corrosion test methods and evaluation criteria

[0077] Table 4.2 Experimental results of a certain essence water

[0078]

[0079] Conclusion: From the test results in Table 4.2, it can be seen that the essence water was tested for fungal growth on the 7th, 14th, 28th and 60th days after inoculation. There was no growth of fungi on the 7th day and no growth within 60 days. All passed the antiseptic challenge test, indicating that the compound with natural antiseptic properties of the present invention has strong antibacterial properties. The prepared essence water is not susceptible to secondary contamination during storage and use, and the physical and chemical performance indicators meet the requirements of relevant quality standards.

[0080] 5. Example 5 Test

[0081] Table 5.1 Application of the composition in Example 5

[0082]

[0083]

[0084] See 1. Example 1 Test for anti-corrosion test methods and evaluation criteria

[0085] Table 5.2 Experimental results of a certain essence water

[0086]

[0087]

[0088] Conclusion: From the test results in Table 5.2, it can be seen that the essence water was tested for fungal growth on the 7th, 14th, 28th and 60th days after inoculation. There was no growth of fungi on the 7th day and no growth within 60 days. All passed the antiseptic challenge test, indicating that the compound with natural antiseptic properties of the present invention has strong antibacterial properties. The prepared essence water is not susceptible to secondary contamination during storage and use, and the physical and chemical performance indicators meet the requirements of relevant quality standards.

[0089] In summary:

[0090] The composition of the present invention contains propylene glycol, lysozyme, hydrolyzed collagen, sodium chloride, tea extract, grapefruit seed extract, and tridecapeptide-1. The hydrolyzed collagen, tridecapeptide-1, and hydrolyzed collagen all have a certain promoting effect on the activity of lysozyme. The structure of hydrolyzed collagen contains α-peptide chains that can form triple helical components, which can provide good support for N-acetyl murein polysaccharide hydrolase, and can also penetrate into the bottom layer of the skin, and has good affinity with surrounding tissues. Tridecapeptide-1 is a polypeptide composed of thirteen amino acids, which has a certain antibacterial effect by itself, and can be synergistically enhanced with lysozyme. Lysozyme, hydrolyzed collagen, and tridecapeptide-1 interact to achieve the effect of 1+1+1>3, and the effect of the single raw material itself is greatly improved through the synergistic effect, and it has the characteristics of naturalness and safety relative to traditional preservatives. Therefore, the composition with natural antiseptic properties of the present invention has a certain antiseptic and antibacterial effect.

Claims

1. A composition having natural antiseptic properties, characterized in that: The composition is composed of the following components by mass percentage: 0.1% to 2% lysozyme, 0.01% to 2% hydrolyzed collagen, 0.01% to 1% sodium chloride, 0.01% to 2% tea extract, 10% to 30% propylene glycol, 0.01% to 1% grapefruit seed extract, 0.01% to 0.02% tridecapeptide-1, and the balance is water. The sum of the mass percentages of the above components is 100%; The preparation method of the composition is as follows: at room temperature, propylene glycol and water are first mixed evenly, and then lysozyme, hydrolyzed collagen, sodium chloride, tea extract, grapefruit seed extract and tridecapeptide-1 are added in sequence, and stirred evenly to obtain the composition.

2. Use of the composition with natural antiseptic properties according to claim 1 in cosmetics, characterized in that: The cosmetics are lotions, emulsions or creams.

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