A molecular marker related to chicken feed utilization and its application

By developing molecular markers based on the PIK3R1 gene and PCR-SSCP technology, the problem of slow progress in chicken feed utilization in traditional genetic selection methods has been solved, enabling rapid and low-cost identification and breeding guidance of chicken feed utilization traits.

CN116356037BActive Publication Date: 2026-05-26ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI AGRICULTURAL UNIVERSITY
Filing Date
2022-09-30
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Traditional genetic breeding methods have made slow progress in improving chicken feed utilization, and molecular marker technology is not widely used in poultry breeding, making it difficult to quickly and effectively detect single nucleotide polymorphisms in the PIK3R1 gene.

Method used

Molecular markers based on the PIK3R1 gene were developed. The nucleotide sequence of the chicken PIK3R1 gene at position 1008 and its upstream and downstream bases was detected using PCR-SSCP technology. Genotyping was performed by non-denaturing polyacrylamide gel electrophoresis and silver staining to determine the feed utilization trait of chickens.

Benefits of technology

This provides a simple, rapid, and low-cost method for early selection of feed utilization traits in chickens, suitable for laboratory applications, and enables efficient identification and breeding guidance of feed utilization traits in chickens.

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Abstract

This invention discloses a molecular marker related to chicken feed utilization and its application, belonging to the technical field of chicken feed utilization identification. The molecular marker is the nucleotide sequence composed of the 1008th base and its upstream and downstream bases in the chicken PIK3R1 gene, where the 1008th base of the PIK3R1 gene is T or G. This invention utilizes the PCR-SSCP method to detect mutations in the PIK3R1 gene and selects chickens based on genotype for feed utilization traits, establishing a breeding method for early selection of poultry feed utilization. This method is simple, rapid, low-cost, and does not require special instruments, making it suitable for the needs of molecular marker-assisted breeding experiments.
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