Composite material containing ultrafine powder of Dendrobium officinale leaves and its application in skin care products

Abstract

The present invention relates to the application field of Dendrobium officinale leaves, and specifically to a composite containing ultrafine Dendrobium officinale leaf powder and its application in skin care products. The ultrafine Dendrobium officinale leaf powder composite comprises ultrafine Dendrobium officinale leaf powder and ultrafine Dendrobium officinale stem powder. The composite of the present invention is mixed in a specific proportion and has a synergistic and complementary effect. Its in vitro antioxidant property far exceeds that of a single raw material under the same conditions. At the same time, it has excellent moisturizing effect and integrates the "anti-aging and moisturizing" functions. It can achieve a great degree of cost reduction under the premise of the same efficacy, improve the market competitiveness of products, and promote the development of related industries.

Description

Technical Field

[0001] The present invention relates to the application field of Dendrobium officinale leaves, and in particular to a composite containing ultrafine powder of Dendrobium officinale leaves and application of the composite in skin care products. Background Art

[0002] Dendrobium officinale is a rare medicinal plant in the genus Dendrobium of the Orchidaceae family. It nourishes yin and promotes fluid production, making it a top-grade yin-nourishing and beauty-enhancing product. Its cooling and moistening properties, combined with its sweet and cold properties, are effective in nourishing yin and promoting fluid production and moistening dryness. It is effective for treating various skin conditions, such as dullness, dryness, roughness, and acne, caused by yin deficiency, excessive fire, and fluid deficiency. However, the complexity of its extraction, limited efficacy, scarcity, and high price have significantly limited its application in cosmetics.

[0003] Dendrobium officinale leaves are the main by-product of its production and processing, often discarded and not fully utilized. Currently, Dendrobium officinale stems and their extracts have been included in the 2021 edition of the "Catalogue of Used Cosmetic Raw Materials," but Dendrobium officinale leaves are rarely used in cosmetics and still have great potential for development.

[0004] Ultrafine grinding technology for traditional Chinese medicine (TCM) refers to a novel processing technique that pulverizes TCM particles into micron- or even nanometer-sized powders. This technology is crucial for improving the utilization rate of active ingredients in TCM, reducing the waste of TCM resources, and promoting the modernization of TCM. Currently, there are few skincare products related to Dendrobium officinale on the market, and the vast majority of these products contain extracts with inconsistent efficacy. Ultrafine grinding technology for TCM is not only simple to use in cosmetics development, but also offers advantages such as high dissolution of active ingredients, rapid dissolution rates, strong skin adhesion, reduced dosage, and improved skin absorption.

[0005] Dendrobium officinale stem ultrafine powder is expensive and has a single effect, and its antioxidant effect needs to be improved, while Dendrobium officinale leaf ultrafine powder is cheap and has a strong antioxidant effect. At present, there is no preparation and application method of Dendrobium officinale leaf ultrafine powder and Dendrobium officinale stem ultrafine powder composite in the art.

[0006] Dendrobium officinale leaves are rich in various active ingredients, including flavonoids, polysaccharides, amino acids, and alkaloids, and possess strong antioxidant and moisturizing properties. However, their application in cosmetics is currently lacking. The stem, the main medicinal part of the plant, is rich in polysaccharides and has some moisturizing properties, but its application in cosmetics is costly, making it difficult to fully promote. Summary of the Invention

[0007] The purpose of the present invention is to provide a Dendrobium officinale leaf ultrafine powder complex with excellent anti-aging performance and excellent moisturizing effect to address the current problems of low utilization rate of Dendrobium officinale leaf resources and the lack of ultrafine powder-related cosmetic products. Another purpose is to provide an application of the above complex in skin care products.

[0008] To achieve the above object, the present invention adopts the following technical solution: a composite containing ultrafine Dendrobium officinale leaf powder, the composite comprising ultrafine Dendrobium officinale leaf powder and ultrafine Dendrobium officinale stem powder.

[0009] In terms of weight percentage, the ratio of the ultrafine powder of Dendrobium officinale leaves to the ultrafine powder of Dendrobium officinale stems is 0.5 to 3.5:1. Preferably, the ratio of the ultrafine powder of Dendrobium officinale leaves to the ultrafine powder of Dendrobium officinale stems is 1.0 to 2.1:1.

[0010] Preferably, the ultrafine powder of Dendrobium officinale leaves and stems is obtained by cutting fresh stems and leaves of Dendrobium officinale, sterilizing them in a high-pressure steam cooker, then vacuum drying them to a constant weight, crushing them with a high-speed ultrafine grinder and passing them through a 300-mesh sieve.

[0011] Preferably, the particle size of the ultrafine powder of Dendrobium officinale leaves and the ultrafine powder of Dendrobium officinale stems ranges from 0.1 to 45 μm.

[0012] Application of a composite containing ultrafine powder of Dendrobium officinale leaves in skin care products.

[0013] The invention discloses an anti-aging lotion containing a composite of ultrafine Dendrobium officinale leaf powder, comprising the following raw materials in percentage by weight: 1-10 parts of the composite of ultrafine Dendrobium officinale leaf powder, 5-10 parts of squalane, 0.5-1 part of glyceryl monostearate, 2-10 parts of PEG-100 stearate, 2-5 parts of glycerin, 0.2-0.5 part of carbomer, 0.2-0.5 part of phenoxyethanol, and 20-30 parts of deionized water.

[0014] An anti-aging lotion containing a superfine Dendrobium officinale leaf powder complex is prepared by heating an oily matrix such as 5-10 parts squalane, 0.5-1 part glyceryl monostearate, and 2-10 parts PEG-100 stearate at 70°C, melting and mixing them uniformly to obtain an oily matrix mixture; heating a water-soluble matrix such as 2-5 parts glycerin, 0.2-0.5 parts carbomer, and 20-30 parts deionized water at 70°C, melting and mixing them uniformly to obtain an aqueous matrix mixture; and mixing 1-10 parts of a complex containing superfine Dendrobium officinale leaf powder and a water-soluble matrix such as 0.2-0.5 parts phenoxyethanol to obtain an active ingredient mixture. The oily matrix mixture is slowly added to the aqueous matrix mixture and stirred uniformly. When the mixture is cooled to 25°C, the active ingredient mixture is poured into the mixture, and the mixture is emulsified for 20 minutes using a homogenizing emulsifier at level 3.

[0015] The Dendrobium officinale leaf ultrafine powder composite provided by the present invention has the following advantages when used in skin care products:

[0016] (1) Pre-treatment is time-saving and simple. Ultrafine grinding of Chinese medicinal materials does not require extraction and purification of active ingredients. The processing operation is simpler, faster and more practical.

[0017] (2) It complies with the modern people’s “green and safe” skin care concept. Compared with traditional medicinal material extraction methods, ultrafine grinding is a purely physical process that can effectively avoid microbial contamination during the extraction process and the irritation of the extraction solvent residue to the skin, and has a higher safety factor.

[0018] (3) Outstanding efficacy. The present invention combines ultrafine powders of Dendrobium officinale leaves and stems in a certain ratio, which exhibit a synergistically enhanced antioxidant effect and excellent moisturizing effect. Optimal efficacy can be achieved while reducing costs.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] After being processed with ultrafine grinding technology, Dendrobium officinale leaves and stems can effectively avoid microbial contamination during the extraction process and irritation to the skin caused by residual extraction solvents. They also have the advantages of strong adhesion to the skin, small dosage, high utilization rate of active ingredients, and easy absorption by the skin.

[0021] When the two ingredients, ultrafine powder of Dendrobium officinale leaves and ultrafine powder of Dendrobium officinale stems, are mixed in a specific proportion, they have a synergistic and complementary effect. Verified by in vitro experiments and human skin tests, the two ingredients, at a specific proportion, have antioxidant properties far exceeding those of a single raw material under the same conditions, and also have excellent moisturizing effects: they can significantly improve skin roughness, reduce the number of pores, wrinkles, brown spots and UV spots, and at the same time significantly increase skin water content in a short period of time, thereby moisturizing the skin.

[0022] The invention makes full use of the Dendrobium officinale leaf resources, reduces production costs, promotes the application of Dendrobium officinale related resources in cosmetics, and has good market development prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 : Comparison of facial skin pores before and after using the emulsion of the present invention;

[0024] Figure 2 : Comparison of facial skin wrinkles before and after using the emulsion of the present invention;

[0025] Figure 3 : Comparison of brown spots on facial skin before and after using the emulsion of the present invention;

[0026] Figure 4: Comparison of facial skin ultraviolet spots before and after using the emulsion of the present invention;

[0027] Figure 5 : Comparison of facial skin roughness before and after using the emulsion of the present invention. DETAILED DESCRIPTION

[0028] The present invention will be further described below with reference to specific embodiments and the accompanying drawings, but the protection scope of the present invention is not limited thereto.

[0029] Example 1-7: Preparation of Dendrobium officinale leaf ultrafine powder, Dendrobium officinale leaf fine powder, Dendrobium officinale stem ultrafine powder, Dendrobium officinale stem fine powder, and a composite of Dendrobium officinale leaf ultrafine powder and the three.

[0030] Example 1 Preparation of Dendrobium officinale leaf ultrafine powder test sample

[0031] Cut the cleaned Dendrobium officinale leaves and sterilize them in a high-pressure steam cooker. Then, vacuum dry them to a constant weight, crush them with a high-speed ultrafine grinder, and pass them through a 300-mesh sieve. Accurately weigh 1g of the powder into a beaker and add distilled water at a solid-liquid ratio of 1:10. After vortexing and mixing, perform ultrasonic extraction for 10 minutes and filter.

[0032] Example 2 Preparation of Dendrobium officinale leaf powder test sample

[0033] Cut the cleaned Dendrobium officinale leaves, sterilize them in a high-pressure steam cooker, and then vacuum-dry them to constant weight. Pulverize them with a standard grinder and pass them through a 100-mesh sieve. Accurately weigh 1g of the powder into a beaker and add distilled water at a solid-liquid ratio of 1:10. After shaking and mixing, ultrasonically extract them for 10 minutes and filter.

[0034] Example 3 Preparation of Dendrobium officinale stem ultrafine powder test sample

[0035] Cut the cleaned stems of Dendrobium officinale and sterilize them in a high-pressure steam cooker. Then, vacuum dry them to a constant weight, crush them with a high-speed ultrafine grinder, and pass them through a 300-mesh sieve. Accurately weigh 1g of powder into a beaker and add distilled water at a solid-liquid ratio of 1:10. After vortexing and mixing, perform ultrasonic extraction for 10 minutes and filter.

[0036] Example 4 Preparation of Dendrobium officinale stem powder test sample

[0037] Cut the cleaned stems of Dendrobium officinale and sterilize them in a high-pressure steam cooker. Then, vacuum dry them to a constant weight, crush them with a standard grinder, and pass them through a 100-mesh sieve. Accurately weigh 1g of the powder into a beaker and add distilled water at a solid-liquid ratio of 1:10. After vortexing and mixing, perform ultrasonic extraction for 10 minutes and filter.

[0038] Example 5 Preparation of Dendrobium officinale Leaf Ultrafine Powder and Dendrobium officinale Leaf Fine Powder Composite

[0039] The ultrafine powder of Dendrobium officinale leaf was prepared in the same manner as in Preparation Example 1, and an equal amount of fine powder of Dendrobium officinale leaf was added (prepared in the same manner as in Preparation Example 2). The two were stirred evenly to obtain a composite sample of ultrafine powder and fine powder of Dendrobium officinale leaf.

[0040] Example 6 Preparation of Dendrobium officinale Leaf Ultrafine Powder and Dendrobium officinale Stem Fine Powder Composite

[0041] The ultrafine powder of Dendrobium officinale leaf was prepared in the same manner as in Preparation Example 1, and an equal amount of the fine powder of Dendrobium officinale stem was added (prepared in the same manner as in Preparation Example 3). The two were stirred evenly to obtain a composite sample of ultrafine powder of Dendrobium officinale leaf and fine powder of Dendrobium officinale stem.

[0042] Example 7 Preparation of Dendrobium officinale Leaf Ultrafine Powder and Dendrobium officinale Stem Ultrafine Powder Composite

[0043] The Dendrobium officinale leaf ultrafine powder test sample was prepared in the same manner as in Preparation Example 1, and an equal amount of the Dendrobium officinale stem ultrafine powder test sample (prepared in the same manner as in Preparation Example 4) was added, and the two were stirred evenly to obtain a Dendrobium officinale leaf ultrafine powder and Dendrobium officinale stem ultrafine powder composite test sample.

[0044] Examples 8-13: Preparation of 6 composites of ultrafine Dendrobium officinale leaf powder and ultrafine Dendrobium officinale stem powder in different proportions.

[0045] Example 8 Preparation of Dendrobium officinale Leaf Ultrafine Powder and Dendrobium officinale Stem Ultrafine Powder Composite

[0046] Prepared in the same manner as in Example 7, the ultrafine powder of Dendrobium officinale leaves and the ultrafine powder of Dendrobium officinale stems were mixed in a ratio of 6:10.

[0047] Example 9 Preparation of Dendrobium officinale Leaf Ultrafine Powder and Dendrobium officinale Stem Ultrafine Powder Composite

[0048] Prepared in the same manner as in Example 7, the ultrafine powder of Dendrobium officinale leaves and the ultrafine powder of Dendrobium officinale stems were mixed in a ratio of 16:12.

[0049] Example 10 Preparation of Dendrobium officinale leaf ultrafine powder and Dendrobium officinale stem ultrafine powder composite

[0050] Prepared in the same manner as Example 7, the ultrafine powder of Dendrobium officinale leaves and the ultrafine powder of Dendrobium officinale stems were mixed in a ratio of 26:18.

[0051] Example 11 Preparation of Dendrobium officinale leaf ultrafine powder and Dendrobium officinale stem ultrafine powder composite

[0052] Prepared in the same manner as in Example 7, the ultrafine powder of Dendrobium officinale leaves and the ultrafine powder of Dendrobium officinale stems were mixed in a ratio of 36:20.

[0053] Example 12 Preparation of Dendrobium officinale Leaf Ultrafine Powder and Dendrobium officinale Stem Ultrafine Powder Composite

[0054] Prepared in the same manner as Example 7, the ultrafine powder of Dendrobium officinale leaves and the ultrafine powder of Dendrobium officinale stems were mixed in a ratio of 46:18.

[0055] Example 13 Preparation of Dendrobium officinale leaf ultrafine powder and Dendrobium officinale stem ultrafine powder composite

[0056] Prepared in the same manner as in Example 7, the ultrafine powder of Dendrobium officinale leaves and the ultrafine powder of Dendrobium officinale stems were mixed in a ratio of 56:16.

[0057] Example 1-13 In vitro antioxidant evaluation experiment

[0058] The DPPH free radical scavenging test, superoxide free radical scavenging test and hydroxyl free radical scavenging test were used to compare the in vitro antioxidant activities of Dendrobium officinale leaf ultrafine powder, Dendrobium officinale leaf fine powder, Dendrobium officinale stem ultrafine powder, Dendrobium officinale stem fine powder and the complex of Dendrobium officinale leaf ultrafine powder and the three.

[0059] Test method:

[0060] DPPH radical scavenging rate: Take the test sample solution from each example and add an equal amount of 0.1 mmol / L DPPH in anhydrous ethanol. Incubate in the dark at room temperature for 30 minutes. Measure the absorbance at 517 nm, which is recorded as A2. A control, using anhydrous ethanol instead of the DPPH-anhydrous ethanol solution, measures the absorbance under the same conditions and procedures, which is recorded as A1. A blank control, using anhydrous ethanol instead of the sample, measures the absorbance and records it as A0. Repeat the experiment three times. Calculate the DPPH radical scavenging rate of the sample: DPPH radical scavenging rate (%) = [1-(A2-A1) / A0] × 100%.

[0061] Superoxide radical scavenging test: Place the prepared Tris-HCl (pH = 8.2) solution in a 25°C water bath for 20 minutes, mix the Tris-HCl solution, the sample solution to be tested, and 25mmol / L pyrogallol solution, and react in a 25°C water bath for 4 minutes. Add 8mmol / L hydrochloric acid solution to terminate the reaction, and measure the absorbance at 310nm, which is recorded as A2; use purified water instead of pyrogallol solution as a control, and measure the absorbance of each sample under the same operation and conditions, which is recorded as A1; the blank control group uses purified water instead of the sample solution, and the absorbance is measured and recorded as A0. Repeat the experiment three times in parallel, and calculate the superoxide radical scavenging rate of the sample: Superoxide radical scavenging rate (%) = [1-(A2-A1) / A0] × 100%

[0062] Hydroxyl radical scavenging rate: Mix 6 mmol / L FeSO₄ solution, the sample to be tested, 0.3% H₂O₂ solution, and 6 mmol / L salicylic acid-ethanol solution and incubate in a 37°C waterbath for 30 min. Measure the absorbance at 510 nm, designated A₂. A control, using purified water instead of the 0.3% H₂O₂ solution, was measured under the same conditions and designated A₁. A blank control, using purified water instead of the sample solution, was measured and designated A₀. Repeat the experiment three times and calculate the hydroxyl radical scavenging rate of the sample: Hydroxyl radical scavenging rate (%) = [1-(A₂-A₁) / A₀] × 100%.

[0063] Table 1 Comparison of in vitro antioxidant properties of Examples 1-7

[0064]

[0065] As shown in Table 1, at the same concentration, Example 7 has the highest DPPH free radical scavenging rate, superoxide free radical scavenging rate, and hydroxyl free radical scavenging rate. This suggests that the combination of ultrafine Dendrobium officinale leaf powder and ultrafine Dendrobium officinale stem powder has a synergistic enhancement effect on in vitro antioxidant activity, and the in vitro antioxidant activity of the combination is stronger than that of using Dendrobium officinale leaf fine powder, Dendrobium officinale stem fine powder, Dendrobium officinale stem ultrafine powder alone, or combining them with the former two separately.

[0066] Table 2 Comparison of in vitro antioxidant properties of Examples 8-13

[0067]

[0068]

[0069] As shown in Table 2, at the same concentration, Examples 8 to 13 all have certain scavenging effects on the DPPH free radical scavenging rate, the superoxide free radical scavenging rate, and the hydroxyl free radical scavenging rate. Among them, Example 9 has the strongest scavenging rate for the DPPH free radical scavenging rate and the hydroxyl free radical scavenging rate. Although the scavenging effect on the superoxide free radical is not as good as that of Example 10, at the same concentration, the scavenging rate of the superoxide free radical still reaches 53.87%, which is higher than that of Examples 1 to 7. This suggests that at this ratio, the composite of the ultrafine powder of Dendrobium officinale leaves and the ultrafine powder of Dendrobium officinale stems can exert a very strong antioxidant effect, and its scavenging rates for DPPH free radicals, superoxide free radicals, and hydroxyl free radicals are 76.34%, 53.87%, and 71.07%, respectively, while the comprehensive antioxidant properties of the other examples are far inferior to that of Example 9.

[0070] In summary, the combination of ultrafine powder of Dendrobium officinale leaves and ultrafine powder of Dendrobium officinale stems in a certain proportion has extremely strong in vitro antioxidant activity, and the complex has the potential to be added to cosmetics as an antioxidant.

[0071] Example 14: An anti-aging lotion containing a Dendrobium officinale leaf ultrafine powder complex

[0072] The preparation method is as follows: according to the amount of material added, 8 parts of squalane, 0.6 parts of glyceryl monostearate, 4 parts of PEG-100 stearate, and other oil phase matrices are heated and melted at 70°C and mixed evenly to obtain an oil phase matrix mixture; 3 parts of glycerin, 0.5 parts of carbomer, and 30 parts of deionized water are heated and melted at 70°C and mixed evenly to obtain an aqueous phase matrix mixture; 8 parts of the composite of ultrafine powder of Dendrobium officinale leaves prepared in Example 9 and 0.3 parts of phenoxyethanol and other water-soluble matrices are mixed evenly to obtain an active ingredient mixture. The oil phase matrix mixture is slowly added to the aqueous phase matrix mixture and stirred evenly; when the mixture is cooled to 25°C, the active ingredient mixture is poured into it and emulsified for 20 minutes using a homogenizing emulsifier at an emulsification speed of level 3.

[0073] Example 15 An anti-aging lotion containing a Dendrobium officinale leaf ultrafine powder complex

[0074] The preparation method is as follows: according to the amount of material added, 6 parts of squalane, 0.5 parts of glyceryl monostearate, 6 parts of PEG-100 stearate and other oil phase matrices are heated to 70°C and melted and mixed evenly to obtain an oil phase matrix mixture; 4 parts of glycerin, 0.4 parts of carbomer, 30 parts of deionized water and other water-soluble matrices are heated to 70°C and melted and mixed evenly to obtain an aqueous phase matrix mixture; 6 parts of the composite of ultrafine powder of Dendrobium officinale leaves prepared in Example 9 and 0.3 parts of phenoxyethanol and other water-soluble matrices are mixed evenly to obtain an active ingredient mixture. The oil phase matrix mixture is slowly added to the aqueous phase matrix mixture and stirred evenly; when the mixture is cooled to 25°C, the active ingredient mixture is poured into it and emulsified for 20 minutes using a homogenizing emulsifier at an emulsification speed of level 3.

[0075] Example 16 An anti-aging lotion containing a Dendrobium officinale leaf ultrafine powder complex

[0076] The preparation method is as follows: according to the amount of material added, 5 parts of squalane, 1 part of glyceryl monostearate, 6 parts of PEG-100 stearate, and other oil phase matrices are heated and melted at 70°C and mixed evenly to obtain an oil phase matrix mixture; 5 parts of glycerin, 0.2 parts of carbomer, and 30 parts of deionized water are heated and melted at 70°C and mixed evenly to obtain an aqueous phase matrix mixture; 10 parts of the composite of ultrafine powder of Dendrobium officinale leaves prepared in Example 9 and 0.5 parts of phenoxyethanol and other water-soluble matrices are mixed evenly to obtain an active ingredient mixture. The oil phase matrix mixture is slowly added to the aqueous phase matrix mixture and stirred evenly; when the mixture is cooled to 25°C, the active ingredient mixture is poured into it and emulsified for 20 minutes using a homogenizing emulsifier at an emulsification speed of level 3.

[0077] Quality evaluation of emulsion containing ultrafine powder of Dendrobium officinale leaves

[0078] Evaluation indicators include: appearance, pH value, cold resistance test, and high temperature resistance test.

[0079] Table 3 Comparison of in vitro antioxidant properties of Examples 14-15

[0080]

[0081] As can be seen from the table, the appearance, pH value, heat resistance, and cold resistance of Examples 14-16 all meet the requirements. This shows that the moisturizing and anti-aging lotion containing the ultrafine Dendrobium officinale leaf powder provided by the present invention can maintain a certain degree of stability at both high and low temperatures, meeting national standards.

[0082] Evaluation of the anti-aging efficacy of a complex containing ultrafine powder of Dendrobium officinale leaves

[0083] Experimental Methods: Fifteen healthy volunteers aged 18 to 60 years with no history of inflammatory skin diseases or allergies were selected for the anti-aging efficacy test. The Dendrobium Officinale Leaf Ultrafine Emulsion was applied daily to the cheeks and forehead for four weeks. Before and after application, the subjects' skin was photographed using a five-light skin chromatograph. A comprehensive statistical analysis was conducted to measure skin roughness, pores, wrinkles, brown spots, and UV spots. The changes in these skin indicators before and after application provided a comprehensive and objective evaluation of the anti-aging efficacy of the Dendrobium Officinale Leaf Ultrafine Powder Complex Emulsion.

[0084] Table 4 Long-term human skin anti-aging efficacy evaluation

[0085]

[0086] Note: Compared with before use * P<0.05, ** P < 0.01;

[0087] As shown in Table 4, after using the emulsion containing ultrafine powder of Dendrobium officinale leaves for 4 weeks, the number of skin pores, wrinkles, UV spots and brown spots of the subjects were significantly reduced. Figure 1-Figure 5 As shown in the figure, the reduction rates were 28.79%, 20.34%, 21.13%, and 7.92%, respectively. The skin roughness was significantly improved, and the overall score was significantly improved, indicating that the lotion can delay and resist skin aging.

[0088] Evaluation of the moisturizing efficacy of a complex containing ultrafine powder of Dendrobium officinale leaves on human body

[0089] Test Methods: Fifteen healthy volunteers aged 18 to 60 years with no history of inflammatory skin diseases or allergies were selected for a short-term skin moisturizing test. A 3 cm x 3 cm test area was marked on the inner forearm of the subject using a water-based marker, with at least 1 cm between each test area. The test product was applied to each test area at a dose of (2.0 ± 0.1) mg / cm² and evenly applied using latex gloves until absorbed. Skin moisture content was measured using a skin tester before application and 1, 2, 3, and 4 hours after application.

[0090] Table 5 Evaluation of short-term moisturizing efficacy of human skin

[0091]

[0092] Note: Compared with before use * P<0.05, ** P<0.01; compared with the moisturizing effect of water at the same time point # P<0.05, ## P<0.01

[0093] Table 5 shows that after application of water, the moisturizing effect was extremely low, and it lost its moisturizing properties after the first hour. However, within 4 hours after application of the emulsion containing the ultrafine powder of Dendrobium officinale leaves, the skin moisture content of the subjects increased significantly, and at the fourth hour, the skin moisture content was still 11.29% higher than before application. This shows that the emulsion has a strong moisturizing effect.

[0094] In summary, the Dendrobium officinale leaf complex of the present invention has extremely strong anti-aging and moisturizing effects, can effectively improve skin roughness, reduce the number of skin pores, wrinkles, UV spots and brown spots, with a reduction rate of up to 30%, and also has strong moisturizing effects.

[0095] The main features and advantages of the present invention are described above. The above embodiments are some embodiments of the present invention, rather than all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

Claims

1. A composite containing ultrafine powder of Dendrobium officinale leaves, characterized in that: The composite comprises ultrafine powder of Dendrobium officinale leaves and ultrafine powder of Dendrobium officinale stems, wherein the weight ratio of the ultrafine powder of Dendrobium officinale leaves to the ultrafine powder of Dendrobium officinale stems is 1:1 to 26:18; The preparation method of the ultrafine powder of Dendrobium officinale leaves and stems is as follows: the cleaned Dendrobium officinale leaves and stems are cut, placed in a high-pressure steam cooker for sterilization, then vacuum-dried to constant weight, crushed with a high-speed ultrafine grinder and passed through a 300-mesh sieve to obtain the powder; The particle size of the contained Dendrobium officinale leaf ultrafine powder and Dendrobium officinale stem ultrafine powder ranges from 0.1 to 45 μm.

2. The composite containing ultrafine powder of Dendrobium officinale leaves according to claim 1, characterized in that The weight ratio of the Dendrobium officinale leaf ultrafine powder to the Dendrobium officinale stem ultrafine powder is 16:

12.

3. Use of the composite containing ultrafine powder of Dendrobium officinale leaves according to any one of claims 1 to 2 in skin care products.

4. An emulsion containing a Dendrobium officinale leaf ultrafine powder complex, characterized in that: By weight, it contains the following components: 1-10 parts of the complex containing ultrafine powder of Dendrobium officinale leaves according to claim 1 or 2, 5-10 parts of squalane, 0.5-1 part of glyceryl monostearate, 2-10 parts of PEG-100 stearate, 2-5 parts of glycerol, 0.2-0.5 parts of carbomer, 0.2-0.5 parts of phenoxyethanol, and 20-30 parts of deionized water.

5. A method for preparing an emulsion containing a Dendrobium officinale leaf ultrafine powder composite as claimed in claim 4, characterized in that: The preparation steps are: (1) Prepare 5-10 parts of squalane, 0.5-1 part of glyceryl monostearate, and 2-10 parts of PEG-100 stearate according to the amount of materials, heat and melt the above oil phase matrix and mix them evenly to obtain an oil phase matrix mixture; (2) Prepare 2-5 parts of glycerol, 0.2-0.5 parts of carbomer, and 20-30 parts of deionized water according to the amount of materials added, heat and melt the above water-soluble matrix, and mix them evenly to obtain an aqueous phase matrix mixture; (3) According to the amount of material added, 1-10 parts of the composite containing ultrafine powder of Dendrobium officinale leaves and 0.2-0.5 parts of phenoxyethanol were mixed evenly to obtain an active ingredient mixture; (4) Slowly add the oil phase matrix mixture to the water phase matrix mixture and stir evenly; then, pour in the active ingredient mixture and homogenize and emulsify to obtain the product.

6. The method for preparing an emulsion containing a superfine powder composite of Dendrobium officinale leaves according to claim 5, characterized in that: The temperature for heating and melting the oil phase matrix and the water phase matrix is ​​70°C.

7. The method for preparing an emulsion containing a superfine powder composite of Dendrobium officinale leaves according to claim 5, characterized in that: After the aqueous phase matrix mixture and the oil phase matrix mixture are mixed and stirred evenly and then cooled to 25°C, the active ingredient phase is added.

8. The method for preparing an emulsion containing a superfine powder composite of Dendrobium officinale leaves according to claim 5, characterized in that: The homogenizing emulsification condition is 20 min.

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