A dandruff-reducing, antipruritic, and soothing composition, its preparation method, and its application.

Abstract

This invention provides an anti-dandruff, anti-itch, and soothing composition, its preparation method, and its application. The composition includes extracts of Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark. This anti-dandruff, anti-itch, and soothing composition can be added to any shampoo and skincare product. Using the extracts of this invention, combined with a reasonable ratio, and prepared through a special method, it achieves a high content of effective ingredients and excellent anti-dandruff, anti-itch, and soothing effects. This invention maximizes the antibacterial and anti-inflammatory effects of the three plants through extraction and fermentation. The resulting composition has significant antibacterial effects, is easy to promote and apply widely in shampoos, and has excellent anti-dandruff and anti-itch effects. It is gentle and comfortable, and has an excellent soothing effect on the skin.

Description

Technical Field

[0001] This invention belongs to the A61K technical field, specifically relating to a soothing composition; dandruff-reducing, antipruritic, and soothing compositions and their applications. Background Technology

[0002] According to incomplete statistics from the World Health Organization, the incidence of dandruff is over 65%. Surveys in my country show that the incidence among adolescents is over 70%, and almost 100% of men will experience dandruff to varying degrees at some point in their lives, with over 5% experiencing severe dandruff that significantly impacts their work and daily life. Current mainstream shampoos are chemical products, and these chemicals can irritate the scalp, exacerbating scalp itching and dandruff. Topical dandruff medications have seen rapid market growth in recent years. Statistics show that total sales of topical dandruff medications reached approximately 18.5 billion yuan in 2010, and have continued to grow in the past two years, with an annual growth rate exceeding 30%. There is a strong market demand from consumers for dandruff removal products.

[0003] Currently, most anti-dandruff shampoos and conditioners on the domestic market use synthetic chemicals such as ZPT (zinc omeprazole) and glycyrrhizin. While these substances do have some anti-dandruff and anti-itch effects, their effectiveness is not ideal, and they can be irritating. Long-term use may affect the scalp and cause other hair problems. Therefore, there is a need for a shampoo composition that effectively reduces dandruff and relieves itching while being gentle on the scalp and hair. Summary of the Invention

[0004] The purpose of this invention is to provide a composition with plant extracts as the core anti-dandruff ingredient, which has the effects of removing dandruff, relieving itching, and soothing.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] The first aspect of the present invention provides a dandruff-removing, antipruritic, and soothing composition comprising Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract, wherein the mass ratio of Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract is 1:(1-6):(1-20).

[0007] Preferably, the composition comprises Lophatherum gracile leaf extract, Andrographis paniculata leaf extract and Phellodendron chinense bark extract, wherein the mass ratio of Lophatherum gracile leaf extract, Andrographis paniculata leaf extract and Phellodendron chinense bark extract is 1:(1-3):(2-5).

[0008] Preferably, the mass ratio of the Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract is 1:1.5:3.6.

[0009] This invention combines three substances—leaf extract of Lophatherum gracile, leaf extract of Andrographis paniculata, and bark extract of Phellodendron chinense—to obtain a composition that has anti-dandruff and anti-itch effects and is gentle on the scalp and hair. It is suitable for addition to shampoos and has a significant anti-dandruff and anti-itch effect.

[0010] A third aspect of the present invention provides a method for preparing the aforementioned anti-dandruff, anti-itch, and soothing composition, characterized by comprising the following steps:

[0011] (1) Extraction

[0012] Preparation of Lophatherum gracile leaf extract: The dried Lophatherum gracile leaves were ground and extracted by heating with a mixture of methanol and ethanol in a volume ratio of 2 to 5:1. The extract was concentrated under normal pressure and dried to obtain Lophatherum gracile leaf extract.

[0013] Preparation of Andrographis paniculata leaf extract: Grind Andrographis paniculata leaves, soak them in distilled water for 30-60 minutes, preheat at 40-50℃ for 1-2 hours, then heat to boiling and maintain for 2-3 hours to obtain the extract, concentrate and dry to obtain Andrographis paniculata leaf extract.

[0014] Preparation of Phellodendron bark extract: Grind Phellodendron bark, add distilled water A, stir well and soak for 1-2 hours, then heat to boiling and maintain for 60-90 minutes, filter to obtain residue and initial extract; add distilled water B to residue, heat to boiling and maintain for 20-30 minutes, filter to obtain re-extract; combine initial extract and re-extract, concentrate and dry to obtain Phellodendron bark extract;

[0015] (2) Fermentation

[0016] Extracts of Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron chinense bark were mixed to obtain a mixture. A carbon source and the mixture were added to sterile water, and the pH was adjusted to 6.9-7.1 using PBS buffer solution. The mixture was then sterilized with ultraviolet light to obtain a fermentation medium. Bacillus subtilis and Acetobacter pasteurellium were inoculated into Gao's medium and cultured for 12-18 hours to obtain bacterial seed solutions. The bacterial seed solutions were inoculated into the fermentation medium and fermented at 35-38°C for 35-45 hours. The mixture was filtered, and the fermentation broth was freeze-dried to obtain a dandruff-reducing, antipruritic, and soothing composition.

[0017] Preferably, the weight ratio of the light bamboo leaf powder to the mixed alcohol is 1:8-15, and the extraction time is 30-60 minutes.

[0018] Preferably, the leaves of the light bamboo are ground and then passed through a 300-400 mesh sieve.

[0019] Preferably, the leaf extract of Lophatherum gracile is extracted using a Soxhlet extractor.

[0020] Preferably, the volume ratio of methanol to ethanol is 3:1, the weight ratio of bamboo leaf powder to mixed alcohol is 1:10, and the extraction time is 40 minutes.

[0021] The Lophatherum gracile leaf extract obtained by this invention using a compound alcohol (methanol and ethanol) exhibits excellent effects. The optimal extraction ratio (methanol to ethanol volume ratio of 3:1) yields the highest concentration of effective anti-dandruff and antipruritic components, resulting in the best efficacy. The flavonoids in Lophatherum gracile leaves possess antibacterial properties, and the extraction method of this invention yields the highest concentration of effective antibacterial components. The inventors also discovered that the amount of alcohol added affects the extraction results.

[0022] Andrographis paniculata, also known as Andrographis paniculata, has the effects of clearing heat and detoxifying, reducing swelling and relieving pain. In recent years, Andrographis paniculata has received increasing application and attention due to its abundant resources and definite curative effects. However, its internal quality is not stable enough and the extraction process still has shortcomings, which to some extent affects the development and application of its products. This invention uses Andrographis paniculata leaves for extraction. The main components of Andrographis paniculata leaves are diterpenoid lactones, and the minor components are flavonoids, which have antibacterial effects. At the same time, the extraction method of this invention yields a large number of effective components. The inventors speculate that the antibacterial effect of andrographolide compounds is related to the position of the double bonds in andrographolide compounds. The extract of this invention contains andrographolide compounds with intracyclic double bonds, which have strong antibacterial effects. At the same time, it can synergistically work with the antibacterial components in other extracts to exert antibacterial effects, greatly improving the antibacterial effect.

[0023] Patent CN 115089516 A discloses a compound plant extract with anti-dandruff and antipruritic effects and its preparation method, and also discloses a method for preparing Phellodendron bark extract. The flavonoids and alkaloids in this extract have antibacterial and anti-inflammatory effects. However, the inventors found that this method was not ideal in the composition of this invention. Patent CN 115089516 A's process of re-extracting the initial extract may result in the loss of some effective antibacterial components. This invention obtains more effective components through a mixture of the initial extract and the re-extraction of the residue. Furthermore, the extraction conditions of this invention also affect the extraction effect.

[0024] Preferably, the volume ratio of Andrographis paniculata leaves to distilled water is 1:10-12; the volume ratio of Phellodendron amurense bark to distilled water A is 1:15-20; and the volume ratio of the dregs to distilled water B is 1:10-12.

[0025] To improve the fermentation effect of the extract, the carbon source is selected from one or more of glucose, fructose, mannose, and galactose; the mass ratio of the carbon source, mixture, and sterile water is (10-20):(10-15):75; the *Acetobacter pasteurellium* is cultured aerobically at a temperature of 35-38°C, an oxygen content of 18-22%, and a humidity of 70-75%; the *Bacillus subtilis* is cultured anaerobicly at a temperature of 35-38°C and a humidity of 70-75%; the inoculum size of *Acetobacter pasteurellium* is 2-5%; the inoculum size of *Bacillus subtilis* is 2-5%.

[0026] Preferably, the leaves of *Andrographis paniculata* and the bark of *Phellodendron chinense* are ground and passed through a 300-400 mesh sieve.

[0027] This invention involves fermenting extracts of Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron chinense bark. These extracts contain cellulose, which easily flocculates to form a gel-like substance, resulting in precipitation and affecting the product's appearance and efficacy. Furthermore, the polysaccharides they contain readily combine with flavonoids in the form of glycosides, reducing the antibacterial effect of the flavonoids. Therefore, the inventors fermented these extracts to improve the antibacterial properties of the components.

[0028] The fourth aspect of the present invention provides the application of the aforementioned anti-dandruff, anti-itch, and soothing composition in the preparation of shampoo.

[0029] Preferably, the amount of the anti-dandruff, anti-itch, and soothing composition added is 0.1 wt% to 10 wt%.

[0030] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0031] This invention provides a soothing composition and its applications for dandruff removal, anti-itch, and soothing. The composition comprises extracts of Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark. This invention provides a dandruff removal, anti-itch, and soothing composition that can be added to any basic shampoo or skincare product. Utilizing the extracts of this invention and a reasonable formulation, a special preparation method yields a high content of active ingredients, resulting in excellent dandruff removal, anti-itch, and soothing effects. This invention maximizes the antibacterial and anti-inflammatory effects of the three plants through extraction and fermentation. The resulting composition exhibits significant antibacterial effects, facilitating widespread application in shampoos. It provides excellent dandruff removal and anti-itch effects, with a gentle and comfortable action and excellent soothing effect on the skin. This invention's dandruff removal, anti-itch, and soothing composition can remove dandruff from its root cause, preventing recurrence, thoroughly cleaning the scalp, and maintaining the scalp's microecological balance. The minimalist formula is suitable for sensitive scalps, keeping users away from scalp itching, ensuring a clean, gentle, and soothing scalp, and maintaining smooth and shiny hair. It is of great significance to the development of anti-dandruff shampoos. Attached Figure Description

[0032] Figure 1 The images show the effects of adding the composition of Example 1 to a shampoo, with the front image showing the effect before use and the back image showing the effect after use.

[0033] Figure 2 The images show the effects of adding the composition of Example 1 to a face cream, with the front image showing the effect before use and the back image showing the effect after use. Detailed Implementation

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] Example 1

[0036] This embodiment provides a dandruff-removing, antipruritic, and soothing composition, which includes Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract in a mass ratio of 1:1.5:3.6.

[0037] The method for preparing the dandruff-reducing, antipruritic, and soothing composition includes the following steps:

[0038] (1) Extraction

[0039] Preparation of Lophatherum gracile leaf extract: The dried Lophatherum gracile leaves were ground and extracted by heating with a mixed alcohol consisting of methanol and ethanol at a volume ratio of 3:1. The extract was concentrated under normal pressure and dried to obtain Lophatherum gracile leaf extract. The weight ratio of Lophatherum gracile powder to the mixed alcohol was 1:10, and the extraction time was 40 minutes.

[0040] Preparation of Andrographis paniculata leaf extract: The leaves of Andrographis paniculata were ground and soaked in distilled water for 40 minutes. After preheating at 45°C for 1.5 hours, the mixture was heated to boiling and maintained for 2.5 hours to obtain the extract. The extract was concentrated and dried to obtain Andrographis paniculata leaf extract. The volume ratio of Andrographis paniculata leaf to distilled water was 1:11.

[0041] Preparation of Phellodendron bark extract: Phellodendron bark was ground, mixed with distilled water, and soaked for 1.5 hours. The mixture was then heated to boiling and maintained for 80 minutes. The residue and initial extract were obtained by filtration. Distilled water was added to the residue again, and the mixture was heated to boiling and maintained for 25 minutes. The extract was then filtered to obtain a second extract. The initial and second extracts were combined, concentrated, and dried to obtain the Phellodendron bark extract. The volume ratio of Phellodendron bark to distilled water was 1:17. The volume ratio of residue to distilled water was 1:11.

[0042] (2) Fermentation

[0043] Extracts from Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark were mixed to obtain a mixture. A carbon source, consisting of glucose and fructose in a weight ratio of 8:2, was added to sterile water. The mass ratio of the carbon source, mixture, and sterile water was 15:13:75. The pH was adjusted to 6.9 using PBS buffer, and the mixture was sterilized by ultraviolet light to obtain the fermentation medium. Bacillus subtilis and Acetobacter pasteurellium were inoculated into Gao's medium, respectively. Acetobacter pasteurellium was cultured aerobically at 37°C, 20% oxygen, and 75% humidity, while Bacillus subtilis was cultured anaerobicly at 37°C in an anaerobic environment with 75% humidity for 14 hours. Seed cultures were prepared with a bacterial count of 120 CFU / mL. The bacterial seed liquid was inoculated into the fermentation medium, with 3% of *Acetobacter pastoris* and 2% of *Bacillus subtilis*. The mixture was fermented at 37°C for 40 hours, filtered, and the fermentation broth was freeze-dried to obtain a dandruff-removing, antipruritic, and soothing composition.

[0044] Example 2

[0045] This embodiment provides a dandruff-removing, antipruritic, and soothing composition, which includes Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract in a mass ratio of 1:1.5:3.6.

[0046] The method for preparing the dandruff-reducing, antipruritic, and soothing composition includes the following steps:

[0047] (1) Extraction

[0048] Preparation of Lophatherum gracile leaf extract: Lophatherum gracile leaves were ground and soaked in distilled water for 40 minutes. After preheating at 45°C for 1.5 hours, the mixture was heated to boiling and maintained for 2.5 hours to obtain the extract. The extract was then concentrated and dried to obtain Lophatherum gracile leaf extract. The volume ratio of Lophatherum gracile leaves to distilled water was 1:11.

[0049] Preparation of Andrographis paniculata leaf extract: The leaves of Andrographis paniculata were ground and soaked in distilled water for 40 minutes. After preheating at 45°C for 1.5 hours, the mixture was heated to boiling and maintained for 2.5 hours to obtain the extract. The extract was concentrated and dried to obtain Andrographis paniculata leaf extract. The volume ratio of Andrographis paniculata leaf to distilled water was 1:11.

[0050] Preparation of Phellodendron bark extract: Phellodendron bark was ground, mixed with distilled water, and soaked for 1.5 hours. The mixture was then heated to boiling and maintained for 80 minutes. The residue and initial extract were obtained by filtration. Distilled water was added to the residue again, and the mixture was heated to boiling and maintained for 25 minutes. The extract was then filtered to obtain a second extract. The initial and second extracts were combined, concentrated, and dried to obtain the Phellodendron bark extract. The volume ratio of Phellodendron bark to distilled water was 1:17. The volume ratio of residue to distilled water was 1:11.

[0051] (2) Fermentation

[0052] Extracts from Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark were mixed to obtain a mixture. A carbon source, consisting of glucose and fructose in a weight ratio of 8:2, was added to sterile water. The mass ratio of the carbon source, mixture, and sterile water was 15:13:75. The pH was adjusted to 6.9 using PBS buffer, and the mixture was sterilized by ultraviolet light to obtain the fermentation medium. Bacillus subtilis and Acetobacter pasteurellium were inoculated into Gao's medium, respectively. Acetobacter pasteurellium was cultured aerobically at 37°C, 20% oxygen, and 75% humidity, while Bacillus subtilis was cultured anaerobicly at 37°C in an anaerobic environment with 75% humidity for 14 hours. Seed cultures were prepared with a bacterial count of 120 CFU / mL. The bacterial seed liquid was inoculated into the fermentation medium, with 3% of *Acetobacter pastoris* and 2% of *Bacillus subtilis*. The mixture was fermented at 37°C for 40 hours, filtered, and the fermentation broth was freeze-dried to obtain a dandruff-removing, antipruritic, and soothing composition.

[0053] Example 3

[0054] This embodiment provides a dandruff-removing, antipruritic, and soothing composition, which includes Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract in a mass ratio of 1:1.5:3.6.

[0055] The method for preparing the dandruff-reducing, antipruritic, and soothing composition includes the following steps:

[0056] (1) Extraction

[0057] Preparation of Lophatherum gracile leaf extract: The dried Lophatherum gracile leaves were ground and extracted with ethanol by heating to obtain an extract. The extract was concentrated under normal pressure and dried to obtain Lophatherum gracile leaf extract. The weight ratio of Lophatherum gracile leaf powder to ethanol was 1:10, and the extraction time was 40 minutes.

[0058] Preparation of Andrographis paniculata leaf extract: The leaves of Andrographis paniculata were ground and soaked in distilled water for 40 minutes. After preheating at 45°C for 1.5 hours, the mixture was heated to boiling and maintained for 2.5 hours to obtain the extract. The extract was concentrated and dried to obtain Andrographis paniculata leaf extract. The volume ratio of Andrographis paniculata leaf to distilled water was 1:11.

[0059] Preparation of Phellodendron bark extract: Phellodendron bark was ground, mixed with distilled water, and soaked for 1.5 hours. The mixture was then heated to boiling and maintained for 80 minutes. The residue and initial extract were obtained by filtration. Distilled water was added to the residue again, and the mixture was heated to boiling and maintained for 25 minutes. The extract was then filtered to obtain a second extract. The initial and second extracts were combined, concentrated, and dried to obtain the Phellodendron bark extract. The volume ratio of Phellodendron bark to distilled water was 1:17. The volume ratio of residue to distilled water was 1:11.

[0060] (2) Fermentation

[0061] Extracts from Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark were mixed to obtain a mixture. A carbon source, consisting of glucose and fructose in a weight ratio of 8:2, was added to sterile water. The mass ratio of the carbon source, mixture, and sterile water was 15:13:75. The pH was adjusted to 6.9 using PBS buffer, and the mixture was sterilized by ultraviolet light to obtain the fermentation medium. Bacillus subtilis and Acetobacter pasteurellium were inoculated into Gao's medium, respectively. Acetobacter pasteurellium was cultured aerobically at 37°C, 20% oxygen, and 75% humidity, while Bacillus subtilis was cultured anaerobicly at 37°C in an anaerobic environment with 75% humidity for 14 hours. Seed cultures were prepared with a bacterial count of 120 CFU / mL. The bacterial seed liquid was inoculated into the fermentation medium, with 3% of *Acetobacter pastoris* and 2% of *Bacillus subtilis*. The mixture was fermented at 37°C for 40 hours, filtered, and the fermentation broth was freeze-dried to obtain a dandruff-removing, antipruritic, and soothing composition.

[0062] Example 4

[0063] This embodiment provides a dandruff-removing, antipruritic, and soothing composition, which includes Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract in a mass ratio of 3:10:1.

[0064] The method for preparing the dandruff-reducing, antipruritic, and soothing composition includes the following steps:

[0065] (1) Extraction

[0066] Preparation of Lophatherum gracile leaf extract: The dried Lophatherum gracile leaves were ground and extracted by heating with a mixed alcohol consisting of methanol and ethanol at a volume ratio of 3:1. The extract was concentrated under normal pressure and dried to obtain Lophatherum gracile leaf extract. The weight ratio of Lophatherum gracile powder to the mixed alcohol was 1:10, and the extraction time was 40 minutes.

[0067] Preparation of Andrographis paniculata leaf extract: The leaves of Andrographis paniculata were ground and soaked in distilled water for 40 minutes. After preheating at 45°C for 1.5 hours, the mixture was heated to boiling and maintained for 2.5 hours to obtain the extract. The extract was concentrated and dried to obtain Andrographis paniculata leaf extract. The volume ratio of Andrographis paniculata leaf to distilled water was 1:11.

[0068] Preparation of Phellodendron bark extract: Phellodendron bark was ground, mixed with distilled water, and soaked for 1.5 hours. The mixture was then heated to boiling and maintained for 80 minutes. The residue and initial extract were obtained by filtration. Distilled water was added to the residue again, and the mixture was heated to boiling and maintained for 25 minutes. The extract was then filtered to obtain a second extract. The initial and second extracts were combined, concentrated, and dried to obtain the Phellodendron bark extract. The volume ratio of Phellodendron bark to distilled water was 1:17. The volume ratio of residue to distilled water was 1:11.

[0069] (2) Fermentation

[0070] Extracts from Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark were mixed to obtain a mixture. A carbon source, consisting of glucose and fructose in a weight ratio of 8:2, was added to sterile water. The mass ratio of the carbon source, mixture, and sterile water was 15:13:75. The pH was adjusted to 6.9 using PBS buffer, and the mixture was sterilized by ultraviolet light to obtain the fermentation medium. Bacillus subtilis and Acetobacter pasteurellium were inoculated into Gao's medium, respectively. Acetobacter pasteurellium was cultured aerobically at 37°C, 20% oxygen, and 75% humidity, while Bacillus subtilis was cultured anaerobicly at 37°C in an anaerobic environment with 75% humidity for 14 hours. Seed cultures were prepared with a bacterial count of 120 CFU / mL. The bacterial seed liquid was inoculated into the fermentation medium, with 3% of *Acetobacter pastoris* and 2% of *Bacillus subtilis*. The mixture was fermented at 37°C for 40 hours, filtered, and the fermentation broth was freeze-dried to obtain a dandruff-removing, antipruritic, and soothing composition.

[0071] Example 5

[0072] This embodiment provides a dandruff-removing, antipruritic, and soothing composition, which includes Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract in a mass ratio of 1:1.5:3.6.

[0073] The method for preparing the dandruff-reducing, antipruritic, and soothing composition includes the following steps:

[0074] (1) Extraction

[0075] Preparation of Lophatherum gracile leaf extract: The dried Lophatherum gracile leaves were ground and extracted by heating with a mixed alcohol consisting of methanol and ethanol at a volume ratio of 3:1. The extract was concentrated under normal pressure and dried to obtain Lophatherum gracile leaf extract. The weight ratio of Lophatherum gracile powder to the mixed alcohol was 1:10, and the extraction time was 40 minutes.

[0076] Preparation of Andrographis paniculata leaf extract: The leaves of Andrographis paniculata were ground and soaked in distilled water for 40 minutes. After preheating at 45°C for 1.5 hours, the mixture was heated to boiling and maintained for 2.5 hours to obtain the extract. The extract was concentrated and dried to obtain Andrographis paniculata leaf extract. The volume ratio of Andrographis paniculata leaf to distilled water was 1:11.

[0077] Preparation of Phellodendron bark extract: Phellodendron bark was ground, mixed with distilled water, and soaked for 1.5 hours. The mixture was then heated to boiling and maintained for 80 minutes. The residue and initial extract were obtained by filtration. Distilled water was added to the residue again, and the mixture was heated to boiling and maintained for 25 minutes. The extract was then filtered to obtain a second extract. The initial and second extracts were combined, concentrated, and dried to obtain the Phellodendron bark extract. The volume ratio of Phellodendron bark to distilled water was 1:17. The volume ratio of residue to distilled water was 1:11.

[0078] (2) A dandruff-reducing, antipruritic, and soothing composition was obtained by mixing Lophatherum gracile leaf extract, Andrographis paniculata leaf extract and Phellodendron chinense bark extract.

[0079] Example 6

[0080] This embodiment provides a dandruff-removing, antipruritic, and soothing composition, which includes Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract in a mass ratio of 1:1.5:3.6.

[0081] The method for preparing the dandruff-reducing, antipruritic, and soothing composition includes the following steps:

[0082] (1) Extraction

[0083] Preparation of Lophatherum gracile leaf extract: The dried Lophatherum gracile leaves were ground and extracted by heating with a mixed alcohol consisting of methanol and ethanol at a volume ratio of 3:1. The extract was concentrated under normal pressure and dried to obtain Lophatherum gracile leaf extract. The weight ratio of Lophatherum gracile powder to the mixed alcohol was 1:10, and the extraction time was 40 minutes.

[0084] Preparation of Andrographis paniculata leaf extract: The leaves of Andrographis paniculata were ground and soaked in distilled water for 40 minutes. After preheating at 45°C for 1.5 hours, the mixture was heated to boiling and maintained for 2.5 hours to obtain the extract. The extract was concentrated and dried to obtain Andrographis paniculata leaf extract. The volume ratio of Andrographis paniculata leaf to distilled water was 1:11.

[0085] Preparation of Phellodendron bark extract: After cutting and washing the Phellodendron bark, add water for the first decoction (the specific operation is: add 10 times the amount of water of the raw material, soak for 1 hour, heat to boiling and decoct for 3 hours, and filter the Phellodendron bark primary extract with a 100-mesh sieve). (2) Second extraction: add water to the primary extract for the second decoction (the specific operation is: add 8 times the amount of water of the primary extract, heat to boiling and decoct for 2 hours, and filter the Phellodendron bark secondary extract with a 100-mesh sieve respectively; let the secondary extract stand for 2 hours, concentrate and dry to obtain Phellodendron bark extract).

[0086] (2) Fermentation

[0087] Extracts from Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark were mixed to obtain a mixture. A carbon source, consisting of glucose and fructose in a weight ratio of 8:2, was added to sterile water. The mass ratio of the carbon source, mixture, and sterile water was 15:13:75. The pH was adjusted to 6.9 using PBS buffer, and the mixture was sterilized by ultraviolet light to obtain the fermentation medium. Bacillus subtilis and Acetobacter pasteurellium were inoculated into Gao's medium, respectively. Acetobacter pasteurellium was cultured aerobically at 37°C, 20% oxygen, and 75% humidity, while Bacillus subtilis was cultured anaerobicly at 37°C in an anaerobic environment with 75% humidity for 14 hours. Seed cultures were prepared with a bacterial count of 120 CFU / mL. The bacterial seed liquid was inoculated into the fermentation medium, with 3% of *Acetobacter pastoris* and 2% of *Bacillus subtilis*. The mixture was fermented at 37°C for 40 hours, filtered, and the fermentation broth was freeze-dried to obtain a dandruff-removing, antipruritic, and soothing composition.

[0088] Example 7

[0089] This embodiment provides a dandruff-removing, antipruritic, and soothing composition, which includes extracts of Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark in a mass ratio of 1:1.5:3.6.

[0090] The method for preparing the dandruff-reducing, antipruritic, and soothing composition includes the following steps:

[0091] (1) Extraction

[0092] Preparation of Lophatherum gracile leaf extract: The dried Lophatherum gracile leaves were ground and extracted by heating with a mixed alcohol consisting of methanol and ethanol at a volume ratio of 3:1. The extract was concentrated under normal pressure and dried to obtain Lophatherum gracile leaf extract. The weight ratio of Lophatherum gracile powder to the mixed alcohol was 1:10, and the extraction time was 40 minutes.

[0093] Preparation of Andrographis paniculata root extract: The root of Andrographis paniculata was ground, soaked in distilled water for 40 minutes, preheated at 45°C for 1.5 hours, then heated to boiling and maintained for 2.5 hours to obtain the extract, which was concentrated and dried to obtain Andrographis paniculata root extract; the volume ratio of Andrographis paniculata leaf to distilled water was 1:11.

[0094] Preparation of Phellodendron bark extract: Phellodendron bark was ground, mixed with distilled water, and soaked for 1.5 hours. The mixture was then heated to boiling and maintained for 80 minutes. The residue and initial extract were obtained by filtration. Distilled water was added to the residue again, and the mixture was heated to boiling and maintained for 25 minutes. The extract was then filtered to obtain a second extract. The initial and second extracts were combined, concentrated, and dried to obtain the Phellodendron bark extract. The volume ratio of Phellodendron bark to distilled water was 1:17. The volume ratio of residue to distilled water was 1:11.

[0095] (2) Fermentation

[0096] Extracts from Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark were mixed to obtain a mixture. A carbon source, consisting of glucose and fructose in a weight ratio of 8:2, was added to sterile water. The mass ratio of the carbon source, mixture, and sterile water was 15:13:75. The pH was adjusted to 6.9 using PBS buffer, and the mixture was sterilized by ultraviolet light to obtain the fermentation medium. Bacillus subtilis and Acetobacter pasteurellium were inoculated into Gao's medium, respectively. Acetobacter pasteurellium was cultured aerobically at 37°C, 20% oxygen, and 75% humidity, while Bacillus subtilis was cultured anaerobicly at 37°C in an anaerobic environment with 75% humidity for 14 hours. Seed cultures were prepared with a bacterial count of 120 CFU / mL. The bacterial seed liquid was inoculated into the fermentation medium, with 3% of *Acetobacter pastoris* and 2% of *Bacillus subtilis*. The mixture was fermented at 37°C for 40 hours, filtered, and the fermentation broth was freeze-dried to obtain a dandruff-removing, antipruritic, and soothing composition.

[0097] Example 8

[0098] This embodiment provides a dandruff-removing, antipruritic, and soothing composition, which includes Lophatherum gracile leaf extract, Andrographis paniculata leaf extract, and Phellodendron chinense bark extract in a mass ratio of 1:1.5:3.6.

[0099] The method for preparing the dandruff-reducing, antipruritic, and soothing composition includes the following steps:

[0100] (1) Extraction

[0101] Preparation of Lophatherum gracile leaf extract: The dried Lophatherum gracile leaves were ground and extracted by heating with a mixed alcohol consisting of methanol and ethanol at a volume ratio of 3:1. The extract was concentrated under normal pressure and dried to obtain Lophatherum gracile leaf extract. The weight ratio of Lophatherum gracile powder to the mixed alcohol was 1:10, and the extraction time was 40 minutes.

[0102] Preparation of Andrographis paniculata leaf extract: The leaves of Andrographis paniculata were ground and soaked in distilled water for 40 minutes. After preheating at 45°C for 1.5 hours, the mixture was heated to boiling and maintained for 2.5 hours to obtain the extract. The extract was concentrated and dried to obtain Andrographis paniculata leaf extract. The volume ratio of Andrographis paniculata leaf to distilled water was 1:15.

[0103] Preparation of Phellodendron bark extract: Phellodendron bark was ground, mixed with distilled water, and soaked for 1.5 hours. The mixture was then heated to boiling and maintained for 80 minutes. The residue and initial extract were obtained by filtration. Distilled water was added to the residue again, and the mixture was heated to boiling and maintained for 25 minutes. The secondary extract was obtained by filtration. The primary and secondary extracts were combined, concentrated, and dried to obtain the Phellodendron bark extract. The volume ratio of Phellodendron bark to distilled water was 1:11; the volume ratio of residue to distilled water was 1:15.

[0104] (2) Fermentation

[0105] Extracts from Lophatherum gracile leaves, Andrographis paniculata leaves, and Phellodendron amurense bark were mixed to obtain a mixture. A carbon source, consisting of glucose and fructose in a weight ratio of 8:2, was added to sterile water. The mass ratio of the carbon source, mixture, and sterile water was 15:13:75. The pH was adjusted to 6.9 using PBS buffer, and the mixture was sterilized by ultraviolet light to obtain the fermentation medium. Bacillus subtilis and Acetobacter pasteurellium were inoculated into Gao's medium, respectively. Acetobacter pasteurellium was cultured aerobically at 37°C, 20% oxygen, and 75% humidity, while Bacillus subtilis was cultured anaerobicly at 37°C in an anaerobic environment with 75% humidity for 14 hours. Seed cultures were prepared with a bacterial count of 120 CFU / mL. The bacterial seed liquid was inoculated into the fermentation medium, with 3% of *Acetobacter pastoris* and 2% of *Bacillus subtilis*. The mixture was fermented at 37°C for 40 hours, filtered, and the fermentation broth was freeze-dried to obtain a dandruff-removing, antipruritic, and soothing composition.

[0106] Performance testing:

[0107] 1. Antibacterial rate

[0108] Most studies have shown that microbiological factors and local immune responses play a major role in the pathogenesis of dandruff. Currently, the overgrowth of microorganisms, primarily Malassezia furfur, is widely considered to be a significant contributing factor.

[0109] The antibacterial test was conducted according to Section 7.3 of the People's Republic of China Light Industry Standard QB / T 2738-2012 "Evaluation Method for Antibacterial and Antimicrobial Effects of Daily Chemical Products"—specifically, the test method for the antibacterial effect of antibacterial daily chemical products (suspension quantitative method). The antibacterial test was used to determine the effectiveness of the tested sample against Malassezia furfur. An antibacterial rate greater than 50% indicates that the product has an antibacterial effect, i.e., an anti-dandruff effect; the higher the antibacterial rate, the better the effect.

[0110] (1) Materials and Methods

[0111] Instruments and equipment

[0112] Autoclave: Shanghai Lichen Bangxi Instrument Technology Co., Ltd., DGL-100B

[0113] Biosafety cabinet: Zhejiang Fuxia Medical Technology Co., Ltd., BSC-1000ⅡA2

[0114] Incubator: Shanghai Jinghong Experimental Equipment Co., Ltd., MJPS-250

[0115] Reagents and materials

[0116] Strains: Malassezia furfur NBCC337308

[0117] Culture medium: Malassezia furfur culture medium base, Shandong Top Biotechnology Co., Ltd.; Tween 40: Shandong Top Biotechnology Co., Ltd.

[0118] Monooleic glycerides: Shandong Top Biotechnology Co., Ltd.

[0119] Laboratory water: Meets the quality requirements for testing culture media and reagents in GB 4789.28-2013.

[0120] (2) Experimental steps

[0121] 1) Sample concentration: 0.1%;

[0122] 2) Add 0.1 mL of Malassezia furfur suspension and mix thoroughly. After 20 min of incubation, dilute appropriately and take 1 mL of each of the 2-3 dilutions into two parallel petri dishes. Add the corresponding culture medium, mix well, and incubate at 30°C for 4 days. Repeat the above experiment three times and take the average. A blank control should also be included.

[0123] 3) After incubation at 30℃ for 4 days, colony counts were performed and the inhibition rate was calculated. The results are shown in Table 1.

[0124]

[0125] In the formula: Ⅰ - average colony count of the control group; Ⅱ - average colony count of the test group; the result is rounded to two decimal places.

[0126] Table 1 Antibacterial rate

[0127]

[0128]

[0129] 2. Use testing

[0130] The products of Examples 1-8 were added to a mixture of water and surfactant at a mass of 6.1%, wherein the water content was 40% and the surfactant was sodium dodecyl sulfate at a content of 59%. The mixture was stirred evenly to obtain the shampoo.

[0131] 160 healthy individuals aged 30-35 were randomly selected and divided into 8 groups. Each group was tested with one example product. The product was used once every two nights for 3 minutes at a water temperature of 42°C. After three consecutive uses, the user's experience was statistically analyzed. The results are shown in Table 2.

[0132] Questionnaire:

[0133] 1. Before using the product, I felt it had an anti-itch effect.

[0134] ○5 points indicates complete agreement.

[0135] ○4 points indicates agreement

[0136] ○3 points indicates both agreement and disagreement.

[0137] ○2 points indicates a degree of disagreement

[0138] ○1 point indicates a completely different opinion

[0139] 2. Before using the product, I felt it had a soothing effect on my scalp.

[0140] ○5 points indicates complete agreement

[0141] ○4 points indicates agreement

[0142] A score of 3 indicates both agreement and disagreement (i.e., neutral).

[0143] ○2 points indicates a degree of disagreement

[0144] ○1 point indicates a completely different opinion

[0145] Table 2 shows the statistical results of the test.

[0146]

[0147] 3. The product from Example 1 was added to a mixture of water and surfactant at a mass fraction of 6.1%, wherein the water content was 40% and the surfactant was sodium lauryl sulfate at a content of 53.9%. The mixture was stirred thoroughly to obtain the shampoo. Individuals with persistent dandruff problems were selected to use the product for two weeks; results were observed. Figure 1 After two weeks of use, no dandruff was found on the scalp, indicating that the dandruff-removing composition of the present invention has a very good dandruff-removing effect.

[0148] 4. Relief Test

[0149] Hyaluronidase Activity Assay: Relevant literature shows that hyaluronic acid is widely present in the skin and other tissues, and its relationship with the skin is very close. High-molecular-weight hyaluronic acid can inhibit inflammatory responses, thus alleviating discomfort caused by skin inflammation. High-molecular-weight hyaluronic acid is degraded by hyaluronidase, resulting in a decrease in its content. This decomposition produces the degradation product N-acetylglucosamine. Measuring the N-acetylglucosamine in the reaction system can indirectly reflect the activity of hyaluronidase. An in vitro hyaluronidase activity inhibition assay was used to evaluate the anti-allergic activity of the samples. Inhibiting hyaluronidase activity can reduce the degradation of hyaluronic acid and alleviate skin sensitivity.

[0150] Detection method: The compositions of Examples 1-8 were mixed with hyaluronidase solution at a concentration of 6.1 wt%. After shaking and mixing, the mixture was incubated at 37°C for 10 min. Hyaluronic acid salt solution was added and incubated at 37°C for 45 min. BSA solution was added and incubation continued for 10 min. OD was then measured. 600nm The hyaluronidase inhibition rate was calculated using the values. See Table 1.

[0151] Table 3 Hyaluronidase activity inhibition rate

[0152] Group Inhibition rate % Example 1 100 Example 2 86 Example 3 82 Example 4 79 Example 5 73 Example 6 81 Example 7 77 Example 8 83

[0153] As can be seen from the examples, the composition of the present invention has a better soothing effect.

[0154] 5. Applications in the skincare industry.

[0155] The compositions obtained in Examples 1-8 were added to the face cream at an addition rate of 5 wt%, and efficacy experiments were conducted.

[0156] The final face cream formula is as follows:

[0157] Water 65.15%, compositions of Examples 1-8 5%, octyl polymethylsiloxane 3%, isononyl isononanoate 3%, glycerol 2%, 1,2-hexanediol 2%, betaine 0.8%, proline 0.1%, glyceryl stearate 1.05%, polydimethylsiloxane 1.35%, mannose 0.019%, cetearyl alcohol 1%, water balance.

[0158] Among them, Example 1 showed the best soothing effect, see details below. Figure 2 .

[0159] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An anti-dandruff, anti-itch, soothing composition characterized in that, The composition comprises Ginkgo biloba leaf extract, Andrographis paniculata leaf extract and Phellodendri Cortex extract in a mass ratio of 1:1.5:3.6; The preparation method of the dandruff-removing, itching-stopping and soothing composition comprises the following steps: (1) Extraction Preparation of Ginkgo biloba leaf extract: dry Ginkgo biloba leaves are ground to obtain Ginkgo biloba powder, which is extracted by heating with a mixed alcohol of methanol and ethanol in a volume ratio of 3:1 to obtain an extract, which is concentrated under normal pressure and dried to obtain the Ginkgo biloba leaf extract; the weight ratio of the Ginkgo biloba powder to the mixed alcohol is 1:10, and the extraction time is 40 minutes; Preparation of Andrographis paniculata leaf extract: Andrographis paniculata leaves are ground and soaked in distilled water for 40 minutes, preheated at 45 DEG C for 1.5 hours, then heated to boiling and kept for 2.5 hours to obtain an extract, which is concentrated and dried to obtain the Andrographis paniculata leaf extract; the volume ratio of the Andrographis paniculata leaves to the distilled water is 1:11; Preparation of Phellodendri Cortex extract: Phellodendri Cortex is ground, stirred with distilled water, soaked for 1.5 hours, then heated to boiling and kept for 80 minutes, and filtered to obtain dregs and a primary extract; the dregs are added with distilled water, heated to boiling and kept for 25 minutes, and filtered to obtain a secondary extract; the primary extract and the secondary extract are combined, concentrated and dried to obtain the Phellodendri Cortex extract; the volume ratio of the Phellodendri Cortex to the distilled water is 1:17; and the volume ratio of the dregs to the distilled water is 1:11; (2) Fermentation The Ginkgo biloba leaf extract, the Andrographis paniculata leaf extract and the Phellodendri Cortex extract are mixed to obtain a mixture, a carbon source and the mixture are added into sterile water, the carbon source is glucose and fructose in a weight ratio of 8:2, the mass ratio of the carbon source, the mixture and the sterile water is 15:13:75, the pH is adjusted to 6.9 by using a PBS buffer solution, and ultraviolet sterilization is performed to obtain a fermentation medium; Bacillus subtilis and Acetobacter pasteurianus are inoculated into a Gause culture medium respectively, the Acetobacter pasteurianus is cultured in an aerobic environment, the temperature is 37 DEG C, the oxygen content is 20%, and the humidity is 75%; the Bacillus subtilis is cultured in an anaerobic environment, the temperature is 37 DEG C, the oxygen content is 0%, and the humidity is 75%; the culture time is 14 hours, and the bacterial seed liquids are obtained; the bacterial seed liquids are inoculated into the fermentation medium, the inoculation amount of the Acetobacter pasteurianus is 3%, and the inoculation amount of the Bacillus subtilis is 2%; the fermentation is performed at 37 DEG C for 40 hours, and the fermentation liquid is filtered and freeze-dried to obtain the dandruff-removing, itching-stopping and soothing composition.

2. The use of the dandruff-removing, itching-stopping and soothing composition in claim 1 in the preparation of shampoo or skin care products.

3. Use according to claim 2, characterized in that, The addition amount of the dandruff-removing, itching-stopping and soothing composition is 0.1wt%-10wt%.

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