Microbial compound inoculants for rapid degradation of kitchen waste and their application methods

Abstract

This invention discloses a microbial compound agent for the rapid degradation of kitchen waste and its application method, comprising a compound microbial body, additives, and a carrier. The compound microbial body includes photosynthetic bacteria, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus amyloliquefaciens, cellulose-degrading bacteria, and lactic acid bacteria, with a colony-forming unit of 1×10⁻⁶. 9 The compound microbial agent contains 8-15% CFU / g of cfu / g and consists of rice straw powder, which accounts for 75-79% of the total compound microbial agent. Additives include peptone, beef extract, yeast powder, and buffers, which account for 6-17% of the total compound microbial agent. *Pseudomonas aeruginosa* can effectively degrade proteins and hydrocarbons in kitchen waste. *Bacillus amyloliquefaciens* can reduce nitrite accumulation, and the two bacteria mutually promote each other's growth, thus reducing odor while degrading kitchen waste.

Description

Technical Field

[0001] This invention relates to the field of kitchen waste degradation technology, and in particular to a microbial compound agent for the rapid degradation of kitchen waste and its application method. Background Technology

[0002] With the continuous growth of society, economy and world population, the amount of food waste is increasing year by year. Currently, more than 1 billion tons of food waste are generated globally each year. The main sources of food waste are daily household life and various catering industries. It is rich in organic matter such as starch, oil and protein, and has high recycling value.

[0003] A search revealed Chinese patent CN114921356A, which discloses a highly efficient composite microbial agent for degrading kitchen waste, its preparation method, and its application. This composite microbial agent includes a degrading agent and a deodorizing agent. The degrading agent includes Bacillus belye spores, Bacillus glaber spores, and Bacillus cereus spores; the deodorizing agent includes Bacillus subtilis spores. All of the above-mentioned strains were independently isolated and preserved by this invention.

[0004] Compared with existing technologies, the invention patent with Chinese patent number CN114921356A has excellent degradation capabilities for proteins, fats, starches, and cellulose, and Bacillus subtilis also has the ability to efficiently remove NH3 and H2S. The composite microbial agent provided by this invention can rapidly degrade kitchen waste without producing odor, achieving the harmless and reduced-volume treatment of kitchen waste.

[0005] However, in actual use, the above-mentioned device can decompose and deodorize kitchen waste by using degradation agents and deodorizing agents in combination, but it lacks the ability to treat kitchen waste. As a result, the actual degradation efficiency of the agents is low. At the same time, the selected bacteria are difficult to achieve the maximum degradation performance in actual use, and the degradation effect on kitchen waste is lower than the theoretical value. Therefore, a microbial compound agent for rapid degradation of kitchen waste and its application method are proposed. Summary of the Invention

[0006] The purpose of this invention is to address the shortcomings of existing technologies by proposing a microbial compound agent for the rapid degradation of kitchen waste and its application method.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] The microbial compound agent for rapid degradation of kitchen waste includes compound microbial cells, additives and carriers. The compound microbial cells include photosynthetic bacteria, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus amyloliquefaciens, cellulose-degrading bacteria and lactic acid bacteria, with a colony forming unit of 1×109 cfu / g. The compound microbial cells account for 8-15% of the total compound microbial agent.

[0009] The carrier is rice straw powder, which accounts for 75-79% of the total amount of compound microbial agent;

[0010] The additives include peptone, beef extract, yeast powder, and buffers, which account for 6-17% of the total compound microbial agent. The use of additives is to enhance the overall activity and nutrients of the compound microbial agent.

[0011] The photosynthetic bacteria are deposited at the China Center for Type Culture Collection, with accession number CCTCC No: M2018604;

[0012] The *Pseudomonas aeruginosa* strain is deposited at the Shanghai Biotechnology Center, strain number: SHBCC D15767;

[0013] The Bacillus amyloliquefaciens described is deposited at the China Center for Type Culture Collection (CCTCC), located in Wuhan, China, on May 28, 2020, with accession number CCTCCNO:M2020153.

[0014] The preservation number of the cellulose-degrading bacteria is CCTCC NO:M 20211094;

[0015] The lactic acid bacteria are deposited at the China General Microbiological Culture Collection Center, with accession number CGMCC No. 4430;

[0016] The Bacillus subtilis strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 25364 and deposit date of July 20, 2022.

[0017] The above technical solution further includes:

[0018] The ratio of photosynthetic bacteria, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus amyloliquefaciens, cellulose-degrading bacteria and lactic acid bacteria was 1:1:(1.2-1.4):2:2:(1.7-1.9):2.3.

[0019] The method for using microbial compound inoculants for the rapid degradation of kitchen waste includes the following steps:

[0020] Step 1: Alkaline treatment of kitchen waste: Pre-treat the waste by placing it in an alkaline environment;

[0021] Step 2: Add bentonite and biochar to the alkaline-treated kitchen waste and mix for 4-6 hours.

[0022] Step 3: Add the prepared compound microbial agent to the kitchen waste and stir to make the kitchen waste and compound microbial agent evenly mixed;

[0023] Step 4: Transport the mixed materials to the fermentation area for stockpiling.

[0024] The alkaline environment treatment time is 25℃ for 24 hours, accompanied by stirring.

[0025] Meanwhile, the alkaline environment is provided by CaO, with the CaO content being 2.8-3.1g per 100g of kitchen waste.

[0026] The mixing time between kitchen waste and compound microbial agent is 3-5 hours, and the ratio between compound microbial agent and kitchen waste is (1.0-1.1):200. Due to the alkali treatment and bentonite, the amount of compound microbial agent used is reduced.

[0027] The stacked material should be kept at a temperature of 40-60℃ and turned over once every 24 hours, for a total of 3 times.

[0028] The purpose of repeatedly turning and mixing the material is to reduce the excessively high internal temperature of the material after prolonged accumulation.

[0029] The present invention has the following beneficial effects:

[0030] 1. In this invention, alkaline treatment of kitchen waste promotes high-solids anaerobic digestion, thereby destroying substrate cells, accelerating the hydrolysis of complex macromolecular organic matter, and promoting the production of volatile fatty acids. Furthermore, the bentonite and biochar added afterward, due to their large surface area and porosity, provide ample habitat for microbial growth, increasing the reaction efficiency between the subsequent microbial compound agent and kitchen waste.

[0031] 2. In this invention, when used, the photosynthetic bacteria rich in the microbial compound can convert nitrosamines into non-toxic compounds. Among them, Pseudomonas aeruginosa can effectively degrade proteins and hydrocarbons in kitchen waste, and Bacillus amyloliquefaciens can reduce the accumulation of nitrites. Furthermore, Bacillus amyloliquefaciens and Pseudomonas aeruginosa have a mutually promoting effect on growth, thereby reducing the generation of odors while degrading kitchen waste. Detailed Implementation

[0032] The present invention will now be clearly and completely described in conjunction with embodiments thereof. Obviously, the described embodiments are merely some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0033] Example 1

[0034] The first step is to place the waste in an alkaline environment for pretreatment. The alkaline environment treatment time is 25℃ for 24 hours, accompanied by stirring.

[0035] Meanwhile, the alkaline environment is provided by CaO, with the CaO content being 2.8-3.1g per 100g of kitchen waste.

[0036] The second step involves adding bentonite and biochar to the alkaline-treated kitchen waste and mixing it for 4-6 hours. The ratio of bentonite to biochar is 3:1, and the bentonite and biochar constitute 3.3-4.7% of the total amount of kitchen waste.

[0037] The SCOD content of the kitchen waste after the above treatment was 735678.1 mg / L, and the N content was 873.2 mg / L.

[0038] Alkali pretreatment promotes the conversion of recalcitrant proteins into soluble proteins, thereby releasing ammonium particles during hydrolysis.

[0039] Example 2

[0040] The waste is placed in an alkaline environment for pretreatment. The alkaline environment treatment time is 25℃ for 24 hours, accompanied by stirring.

[0041] Meanwhile, the alkaline environment is provided by CaO, with the CaO content being 2.8-3.1g per 100g of kitchen waste.

[0042] The SCOD content of the kitchen waste after the above treatment was 61337.7 mg / L, and the N content was 617.4 mg / L.

[0043] Comparative Example 1

[0044] Pile the kitchen waste together and mix it initially for 5-6 hours.

[0045] Example 3

[0046] The prepared compound microbial agent was added to the kitchen waste treated in Example 1 and stirred to make the kitchen waste and compound microbial agent evenly mixed.

[0047] The mixing time between kitchen waste and compound microbial agent is 3-5 hours, and the ratio between compound microbial agent and kitchen waste is (1.0-1.1):200.

[0048] The mixed materials are transported to the fermentation area for stacking. The temperature of the stacked materials is maintained at 40-60℃, and the mixture is turned over once every 24 hours, for a total of 3 times.

[0049] The mixture comprises a compound bacterial culture, additives, and a carrier. The compound bacterial culture includes photosynthetic bacteria, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus amyloliquefaciens, cellulose-degrading bacteria, and lactic acid bacteria, with a colony-forming unit of 1 × 10⁻⁶. 9 CFU / g, with the compound microbial cells accounting for 8-15% of the total compound microbial agent;

[0050] The carrier is rice straw powder, which accounts for 75-79% of the total amount of compound microbial agent;

[0051] The additives include peptone, beef extract, yeast powder, and buffers, which account for 6-17% of the total compound microbial agent.

[0052] The ratio of photosynthetic bacteria, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus amyloliquefaciens, cellulose-degrading bacteria and lactic acid bacteria was 1:1:(1.2-1.4):2:2:(1.7-1.9):2.3.

[0053] Example 4

[0054] The prepared compound microbial agent was added to the kitchen waste treated in Example 2 and stirred to make the kitchen waste and compound microbial agent evenly mixed.

[0055] The mixing time between kitchen waste and compound microbial agent is 3-5 hours, and the ratio between compound microbial agent and kitchen waste is (1.0-1.1):200.

[0056] The mixed materials are then transported to the fermentation area for stockpiling. The stockpiled materials are maintained at a temperature of 40-60℃ and are turned over every 24 hours, for a total of 3 times.

[0057] Comparative Example 2

[0058] The prepared compound microbial agent was added to the kitchen waste treated in Comparative Example 1 and stirred to ensure that the kitchen waste and compound microbial agent were mixed evenly.

[0059] The mixing time between kitchen waste and compound microbial agent is 3-5 hours, and the ratio between compound microbial agent and kitchen waste is (1.0-1.1):200.

[0060] The mixed materials are transported to the fermentation area for stacking. The temperature of the stacked materials is maintained at 40-60℃, and the mixture is turned over once every 24 hours, for a total of 3 times.

[0061] Starch weight loss rate % 80.49 78.42 74.31 Weight loss rate of leafy green vegetables (%) 80.31 76.23 74.11 Overall weight loss rate 81.36 76.29 69.31 Odor Level slight slight slight

[0062] The alkaline treatment of kitchen waste promotes high-solids anaerobic digestion, thereby destroying substrate cells, accelerating the hydrolysis of complex macromolecular organic matter, and promoting the production of volatile fatty acids. Furthermore, the added bentonite and biochar, due to their large surface area and porosity, provide ample habitat for microbial growth, increasing the reaction efficiency between the subsequent microbial compound agent and kitchen waste.

[0063] The photosynthetic bacteria in the microbial compound can convert nitrosamines into non-toxic compounds. Among them, Pseudomonas aeruginosa can effectively degrade proteins and hydrocarbons in kitchen waste, while Bacillus amyloliquefaciens can reduce the accumulation of nitrites. Furthermore, Bacillus amyloliquefaciens and Pseudomonas aeruginosa promote each other's growth, thereby reducing odors while degrading kitchen waste.

[0064] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for using a microbial compound inoculant for the rapid degradation of kitchen waste, characterized in that, Includes the following steps: Step 1: Alkaline treatment of kitchen waste: Place the kitchen waste in an alkaline environment for pretreatment. The alkaline environment treatment time is 25℃ for 24 hours, with stirring. The alkaline environment is provided by CaO, and the CaO content is 2.8-3.1g CaO per 100g of kitchen waste. Step 2: Add bentonite and biochar to the alkali-treated kitchen waste and mix for 4-6 hours. The ratio of bentonite to biochar should be 3:1, and the bentonite and biochar should account for 3.3-4.7% of the total amount of kitchen waste. Step 3: Add the prepared compound microbial agent to the kitchen waste and stir. The stirring time between the kitchen waste and the compound microbial agent is 3-5 hours to ensure that the kitchen waste and the compound microbial agent are mixed evenly. The ratio between the compound microbial agent and the kitchen waste is (1.0-1.1):

200. The compound microbial agent comprises compound microbial cells, additives, and a carrier. The compound microbial cells include photosynthetic bacteria, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus amyloliquefaciens, cellulose-degrading bacteria, and lactic acid bacteria, with a colony-forming unit of 1 × 10⁻⁶. 9 CFU / g, with the compound microbial cells accounting for 8-15% of the total compound microbial agent; The carrier is rice straw powder, which accounts for 75-79% of the total amount of compound microbial agent; The additives include peptone, beef extract, yeast powder, and buffers, which account for 6-17% of the total compound microbial agent. The ratio of photosynthetic bacteria, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus amyloliquefaciens, cellulose-degrading bacteria, and lactic acid bacteria in the complex bacterial culture is 1:1:(1.2-1.4):2:2:(1.7-1.9):2.

3. Step 4: Transport the mixed materials to the fermentation area for stacking. The temperature of the stacked materials should be maintained at 40-60℃, and the mixture should be turned over once every 24 hours, for a total of 3 times.

2. The method of using the microbial compound agent for rapid degradation of kitchen waste according to claim 1, characterized in that, The photosynthetic bacteria are deposited at the China Center for Type Culture Collection, with accession number CCTCC No: M 2018604; The Bacillus subtilis strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 25364 and deposit date of July 20, 2022. The *Pseudomonas aeruginosa* strain is deposited at the Shanghai Biotechnology Center, strain number: SHBCC D15767; The Bacillus amyloliquefaciens described is deposited at the China Center for Type Culture Collection (CCTCC), located in Wuhan, China, on May 28, 2020, with accession number CCTCCNO:M2020153. The preservation number of the cellulose-degrading bacteria is CCTCC NO:M20211094; The lactic acid bacteria are deposited at the China General Microbiological Culture Collection Center, with accession number CGMCC No. 4430.